CA2614062A1 - Novel genes involved in petroselinic acid biosynthesis and method for producing petroselinic acid - Google Patents

Novel genes involved in petroselinic acid biosynthesis and method for producing petroselinic acid Download PDF

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Publication number
CA2614062A1
CA2614062A1 CA002614062A CA2614062A CA2614062A1 CA 2614062 A1 CA2614062 A1 CA 2614062A1 CA 002614062 A CA002614062 A CA 002614062A CA 2614062 A CA2614062 A CA 2614062A CA 2614062 A1 CA2614062 A1 CA 2614062A1
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Prior art keywords
gene
protein
acid
gene according
producing
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Granted
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CA002614062A
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French (fr)
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CA2614062C (en
Inventor
Yukio Okamura
Yoko Yamanaka
Nobuhiko Muramoto
Ikuo Nishida
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Toyota Motor Corp
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Individual
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Priority to CA2700545A priority Critical patent/CA2700545C/en
Publication of CA2614062A1 publication Critical patent/CA2614062A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids

Abstract

Disclosed are a novel gene capable of promoting the accumulation of petroselinic acid and a process for producing petroselinic acid using the gene. A gene encoding any one of the following proteins (a), (b) and (c): (a) a protein comprising the amino acid sequence depicted in SEQ ID NO:4 or 6; (b) a protein which comprises an amino acid sequence having the deletion, substitution or addition of one or more amino acid residues in the amino acid sequence depicted in SEQ ID NO:4 or 6 and which has a petroselinoyl-ACP
thioesterase activity; and (c) a protein which is encoded by DNA capable of hybridizing with DNA comprising a nucleotide sequence complementary to the nucleotide sequence depicted in SEQ ID NO:3 or 5 under stringent conditions and which has a petroselinoyl-ACP thioesterase activity.

Claims (13)

1. A gene encoding the following protein (a), (b), or (c):

(a) a protein that comprises the amino acid sequence shown in SEQ ID NO: 2;

(b) a protein that comprises an amino acid sequence shown in SEQ ID NO: 2 including deletion, substitution, or addition of one or a plurality of amino acids and has .DELTA.4-palmitoyl-ACP desaturase activity; or (c) a protein that is encoded by a DNA hybridizing under stringent conditions to a DNA
comprising a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 1 and has .DELTA.4-palmitoyl-ACP desaturase activity.
2. A gene encoding the following protein (a), (b), or (c):

(a) a protein that comprises the amino acid sequence shown in SEQ ID NO: 4 or 6;

(b) a protein that comprises an amino acid sequence shown in SEQ ID NO: 4 or 6 including deletion, substitution, or addition of one or a plurality of amino acids and has petroselinoyl-ACP thioesterase activity; or (c) a protein that is encoded by a DNA hybridizing under stringent conditions to a DNA
comprising a nucteotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 3 or 5 and has petroselinoyl-ACP thioesterase activity.
3. An expression vector having the gene according to claim 1 and/or the gene according to claim 2.
4. A transformant, into which the gene according to claim 1 and/or the gene according to claim 2 is introduced.
5. A method for producing petroselinic acid, comprising the steps of:

introducing the gene according to claim 1 and/or the gene according to claim 2 into a plant cell, so as to produce a transformed plant; and extracting petroselinic acid from tissue that is collected from the transformed plant.
6. A transformed plant cell or transformed plant capable of producing petroselinic acid, into which the gene according to claim 1 and the gene according to 2 are introduced.
7. A transformed plant cell or transformed plant capable of producing cis-4-hexadecenoic acid, into which the gene according to claim 1 and the gene according to claim 2 are introduced.
8. A transformed plant cell or transformed plant capable of producing cis-8-icosenoic acid, into which the gene according to claim 1 and the gene according to claim 2 are introduced.
9. A transformed plant cell or an individual transformed plant capable of increasing the production of saturated fatty acid, into which the gene according to claim 2 is introduced.
10. The method for producing petroselinic acid according to claim 5, comprising transforming the plant cell with the use of an expression vector having a seed-specific promoter and the gene located downstream of the promoter and then extracting the petroselinic acid from seeds.
11. The method for producing cis-4-hexadecenoic acid according to claim 5, comprising transforming the plant cell with the use of an expression vector having a seed-specific promoter and the gene located downstream of the promoter and then extracting the cis-4-hexadecenoic acid from seeds.
12. The method for producing cis-8-icosenoic acid according to claim 5, comprising transforming the plant cell with the use of an expression vector having a seed-specific promoter and the gene located downstream of the promoter and then extracting the cis-8-icosenoic acid from seeds.
13. The method for producing saturated fatty acid according to claim 5, comprising transforming the plant cell with the use of an expression vector having a seed-specific promoter and the gene located downstream of the promoter and then extracting the saturated fatty acid from seeds.
CA2614062A 2005-06-30 2006-06-29 Novel genes involved in petroselinic acid biosynthesis and method for producing petroselinic acid Active CA2614062C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2700545A CA2700545C (en) 2005-06-30 2006-06-29 Novel genes involved in petroselinic acid biosynthesis and method for producing petroselinic acid

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2005-191775 2005-06-30
JP2005191775 2005-06-30
PCT/JP2006/313449 WO2007004694A1 (en) 2005-06-30 2006-06-29 Novel gene involved in biosynthesis of petroselinic acid and process for production of petroselinic acid

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2700545A Division CA2700545C (en) 2005-06-30 2006-06-29 Novel genes involved in petroselinic acid biosynthesis and method for producing petroselinic acid

Publications (2)

Publication Number Publication Date
CA2614062A1 true CA2614062A1 (en) 2007-01-11
CA2614062C CA2614062C (en) 2011-11-15

Family

ID=37604552

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2614062A Active CA2614062C (en) 2005-06-30 2006-06-29 Novel genes involved in petroselinic acid biosynthesis and method for producing petroselinic acid
CA2700545A Active CA2700545C (en) 2005-06-30 2006-06-29 Novel genes involved in petroselinic acid biosynthesis and method for producing petroselinic acid

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2700545A Active CA2700545C (en) 2005-06-30 2006-06-29 Novel genes involved in petroselinic acid biosynthesis and method for producing petroselinic acid

Country Status (5)

Country Link
JP (1) JP4840360B2 (en)
CN (1) CN101213298B (en)
AU (1) AU2006266760B2 (en)
CA (2) CA2614062C (en)
WO (1) WO2007004694A1 (en)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5430134A (en) * 1992-08-07 1995-07-04 Michigan State University Method for production of petroselinic acid and OMEGA12 hexadecanoic acid in transgenic plants
DK0778896T3 (en) * 1994-08-31 2015-04-07 Du Pont Nucleotide OF RAPE AND SOYA BEAN palmitoyl-ACP THIOESTERASEGENER AND USE THEREOF IN REGULATION OF FATTY ACID CONTENT oils FROM soybean and RAPS PLANTS
US6150512A (en) * 1995-05-15 2000-11-21 Yuan; Ling Engineering plant thioesterases and disclosure of plant thioesterases having novel substrate specificity
WO1997012047A1 (en) * 1995-09-29 1997-04-03 Calgene, Inc. Plant stearoyl-acp thioesterase sequences and methods to increase stearate content in plant seed oils
US6281375B1 (en) * 1998-08-03 2001-08-28 Cargill, Incorporated Biodegradable high oxidative stability oils
GB9918022D0 (en) * 1999-07-30 1999-09-29 Unilever Plc Skin care composition

Also Published As

Publication number Publication date
JPWO2007004694A1 (en) 2009-01-29
JP4840360B2 (en) 2011-12-21
CN101213298B (en) 2012-04-18
CA2614062C (en) 2011-11-15
AU2006266760B2 (en) 2011-12-15
WO2007004694A1 (en) 2007-01-11
CA2700545C (en) 2012-08-28
AU2006266760A1 (en) 2007-01-11
CN101213298A (en) 2008-07-02
CA2700545A1 (en) 2007-01-11

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