CA2612052A1 - Method and devices for forming a plurality of wells on a gel - Google Patents
Method and devices for forming a plurality of wells on a gel Download PDFInfo
- Publication number
- CA2612052A1 CA2612052A1 CA002612052A CA2612052A CA2612052A1 CA 2612052 A1 CA2612052 A1 CA 2612052A1 CA 002612052 A CA002612052 A CA 002612052A CA 2612052 A CA2612052 A CA 2612052A CA 2612052 A1 CA2612052 A1 CA 2612052A1
- Authority
- CA
- Canada
- Prior art keywords
- gel
- top plate
- sheet
- analyte
- template
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 64
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 51
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 43
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 43
- 239000012491 analyte Substances 0.000 claims abstract description 42
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 238000000926 separation method Methods 0.000 claims abstract description 21
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 19
- 238000001155 isoelectric focusing Methods 0.000 claims abstract description 17
- 239000000203 mixture Substances 0.000 claims abstract description 10
- 150000001720 carbohydrates Chemical class 0.000 claims abstract description 7
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 7
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 7
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 7
- 238000001962 electrophoresis Methods 0.000 claims description 14
- 239000004033 plastic Substances 0.000 claims description 11
- 229920003023 plastic Polymers 0.000 claims description 11
- 238000003780 insertion Methods 0.000 claims description 6
- 230000037431 insertion Effects 0.000 claims description 6
- 229920002401 polyacrylamide Polymers 0.000 claims description 6
- 108091005804 Peptidases Proteins 0.000 claims description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 239000000499 gel Substances 0.000 abstract description 117
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 30
- 235000014633 carbohydrates Nutrition 0.000 abstract description 5
- 239000002585 base Substances 0.000 description 43
- 239000000523 sample Substances 0.000 description 13
- 238000013459 approach Methods 0.000 description 12
- 230000029087 digestion Effects 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 8
- 238000000605 extraction Methods 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 239000012528 membrane Substances 0.000 description 7
- 238000012545 processing Methods 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 6
- 238000012546 transfer Methods 0.000 description 6
- 239000002985 plastic film Substances 0.000 description 5
- 238000011084 recovery Methods 0.000 description 4
- 239000000872 buffer Substances 0.000 description 3
- 238000012864 cross contamination Methods 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 238000004949 mass spectrometry Methods 0.000 description 3
- 238000001819 mass spectrum Methods 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 108010029485 Protein Isoforms Proteins 0.000 description 2
- 102000001708 Protein Isoforms Human genes 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 239000007769 metal material Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 238000004885 tandem mass spectrometry Methods 0.000 description 2
- 238000000539 two dimensional gel electrophoresis Methods 0.000 description 2
- 101100136092 Drosophila melanogaster peng gene Proteins 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 101100384355 Mus musculus Ctnnbip1 gene Proteins 0.000 description 1
- 102000007079 Peptide Fragments Human genes 0.000 description 1
- 108010033276 Peptide Fragments Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 108010026552 Proteome Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000002349 difference gel electrophoresis Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002330 electrospray ionisation mass spectrometry Methods 0.000 description 1
- 238000002101 electrospray ionisation tandem mass spectrometry Methods 0.000 description 1
- 238000000295 emission spectrum Methods 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000009144 enzymatic modification Effects 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000001906 matrix-assisted laser desorption--ionisation mass spectrometry Methods 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44795—Isoelectric focusing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0512511.7 | 2005-06-18 | ||
| GB0512511A GB0512511D0 (en) | 2005-06-18 | 2005-06-18 | Method and devices for forming a plurity of wells on a gell |
| GB0608141A GB0608141D0 (en) | 2006-04-25 | 2006-04-25 | Method and devices for forming a plurality of wells on a gel |
| GB0608141.8 | 2006-04-25 | ||
| PCT/EP2006/005530 WO2006136296A1 (en) | 2005-06-18 | 2006-06-09 | Method and devices for forming a plurality of wells on a gel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2612052A1 true CA2612052A1 (en) | 2006-12-28 |
Family
ID=36954367
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002612052A Abandoned CA2612052A1 (en) | 2005-06-18 | 2006-06-09 | Method and devices for forming a plurality of wells on a gel |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20100213065A1 (https=) |
| EP (1) | EP1917522A1 (https=) |
| JP (1) | JP2008544232A (https=) |
| AU (1) | AU2006261308A1 (https=) |
| CA (1) | CA2612052A1 (https=) |
| WO (1) | WO2006136296A1 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9341595B2 (en) * | 2010-01-11 | 2016-05-17 | Ge Healthcare Bio-Sciences Ab | Electrophoresis gel assembly |
| US9766207B2 (en) * | 2011-11-04 | 2017-09-19 | Bio-Rad Laboratories, Inc. | Affinity methods and compositions employing electronic control of pH |
| JP5975636B2 (ja) * | 2011-12-20 | 2016-08-23 | シャープ株式会社 | 電気泳動用カセット、電気泳動用カセットの製造方法、および電気泳動方法 |
| GB201322081D0 (en) * | 2013-12-13 | 2014-01-29 | Ge Healthcare Bio Sciences Ab | Circular extractor |
| WO2016062231A1 (zh) * | 2014-10-21 | 2016-04-28 | 鲍坚斌 | 凝胶电泳芯片 |
| KR20240157217A (ko) | 2023-04-25 | 2024-11-01 | 정인호 | 모기퇴치와 수면기능을 가진 로션 |
| WO2025230863A1 (en) * | 2024-04-29 | 2025-11-06 | ProteinSimple | Systems and methods for improved tracking, fractionation, collection, and identification of analytes in a sample |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3631575A (en) * | 1970-06-01 | 1972-01-04 | Walter Farris | Multiwell agar gel punch |
| US4181594A (en) * | 1979-03-27 | 1980-01-01 | University Of Pittsburgh | Matrix recovery electrophoresis apparatus |
| US4834946A (en) * | 1987-02-05 | 1989-05-30 | Levin Andrew E | Apparatus for blot screening numerous, small volume, antibody solutions |
| US5217591A (en) * | 1990-05-14 | 1993-06-08 | Labintelligence, Inc. | Gel electrophoresis sample applicator/retriever |
| US5340449A (en) * | 1990-12-07 | 1994-08-23 | Shukla Ashok K | Apparatus for electroelution |
| AU1324192A (en) * | 1992-02-25 | 1993-09-13 | Peter Andersen | Process for electroelution of a gel containing separated charged macromolecules, such as proteins or DNA/RNA, and an apparatus and means for use in the process |
| DE19815400C2 (de) * | 1998-04-06 | 2002-02-07 | Max Planck Gesellschaft | Vorrichtung zur Probenaufnahme aus polymeren Trägermaterialien |
| DE19926985B4 (de) * | 1999-06-14 | 2004-12-02 | Richard Dr. Reinhardt | Verfahren und Vorrichtung zur Gelelektrophorese |
| US6342143B1 (en) * | 2000-01-06 | 2002-01-29 | Carnegie Mellon University | Cutting tool for multiple sample retrieval from gelatinous material |
| US6652724B2 (en) * | 2001-04-04 | 2003-11-25 | Large Scale Proteomics Corporation | Automated apparatus for separating a biological sample from a two dimensional electrophoresis gel |
| JP4201596B2 (ja) * | 2000-12-18 | 2008-12-24 | プロテダイン・コーポレーション | 自動化ラボラトリシステム及び方法 |
| AU2002353107A1 (en) * | 2001-12-11 | 2003-07-09 | Sau Lan Tang Staats | Microfluidic devices and methods for two-dimensional separations |
| DE10252177A1 (de) * | 2002-11-09 | 2004-06-09 | Roche Diagnostics Gmbh | Trennvorrichtung und Trennverfahren für biomolekulares Probenmaterial |
| US7025864B2 (en) * | 2003-03-10 | 2006-04-11 | Elchrom Scientific A.G. | Method and apparatus for recovering target molecules from a gel containing said target molecules |
| EP1668351A1 (en) * | 2003-09-24 | 2006-06-14 | Agilent Technologies Inc | Extraction of molecules using frame |
-
2006
- 2006-06-09 JP JP2008516190A patent/JP2008544232A/ja active Pending
- 2006-06-09 EP EP06754250A patent/EP1917522A1/en not_active Withdrawn
- 2006-06-09 WO PCT/EP2006/005530 patent/WO2006136296A1/en not_active Ceased
- 2006-06-09 AU AU2006261308A patent/AU2006261308A1/en not_active Abandoned
- 2006-06-09 CA CA002612052A patent/CA2612052A1/en not_active Abandoned
- 2006-06-09 US US11/917,909 patent/US20100213065A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2006136296A1 (en) | 2006-12-28 |
| AU2006261308A1 (en) | 2006-12-28 |
| JP2008544232A (ja) | 2008-12-04 |
| US20100213065A1 (en) | 2010-08-26 |
| EP1917522A1 (en) | 2008-05-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kilár | Recent applications of capillary isoelectric focusing | |
| Rabilloud | Two‐dimensional gel electrophoresis in proteomics: old, old fashioned, but it still climbs up the mountains | |
| Zuo et al. | Towards global analysis of mammalian proteomes using sample prefractionation prior to narrow pH range two‐dimensional gels and using one‐dimensional gels for insoluble and large proteins | |
| Zuo et al. | Comprehensive analysis of complex proteomes using microscale solution isoelectrofocusing prior to narrow pH range two‐dimensional electrophoresis | |
| Manabe | Capillary electrophoresis of proteins for proteomic studies | |
| US20070199821A1 (en) | Automated two-dimensional gel electrophoresis | |
| JP2004500567A (ja) | タンパク質分離および提示 | |
| US6676819B1 (en) | Methods and apparatus for automatic on-line multi-dimensional electrophoresis | |
| US20040033591A1 (en) | Automated protein analysis system | |
| US20100213065A1 (en) | Method and devices for forming a plurality of wells on a gel | |
| Walsh et al. | The Australian Proteome Analysis Facility (APAF): assembling large scale proteomics through integration and automation | |
| EP1587840B1 (en) | Method to form a protein microarray system | |
| US7989215B2 (en) | Methods and systems for adding a reagent to an analyte in a gel | |
| US20050230315A1 (en) | Protein microarray system | |
| US20080096284A1 (en) | Protein separation and analysis | |
| EP1668350B1 (en) | Electrophoretic separation of amphoteric molecules | |
| EP3079821B1 (en) | Circular extractor | |
| EP1393061A4 (en) | METHODS OF ANALYZING MULTIPLE PHASE PROTEINS | |
| US20080153711A1 (en) | Protein microarray system | |
| Wong et al. | Peptide mapping of 2-D gel proteins by capillary HPLC | |
| Simon | Isoelectric focusing of iTRAQ-labeled yeast | |
| Liebler | Analytical Protein and Peptide Separations | |
| EP1835288A1 (en) | Method for the analysis of naturally occuring peptides and small proteins in cells and solid biological materials using mass spectrometry | |
| Gaertner et al. | Increasing Throughput and Data Quality for Proteomics | |
| Wittmann-Liebold et al. | Two-Dimensional Electrophoresis and Automated Microsequencing: Introduction and Overview |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |