CA2589651A1 - Multi-wavelength fluorometric system and probe for monitoring of bioprocesses - Google Patents
Multi-wavelength fluorometric system and probe for monitoring of bioprocesses Download PDFInfo
- Publication number
- CA2589651A1 CA2589651A1 CA 2589651 CA2589651A CA2589651A1 CA 2589651 A1 CA2589651 A1 CA 2589651A1 CA 2589651 CA2589651 CA 2589651 CA 2589651 A CA2589651 A CA 2589651A CA 2589651 A1 CA2589651 A1 CA 2589651A1
- Authority
- CA
- Canada
- Prior art keywords
- light source
- light
- sample
- probe
- sources
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/85—Investigating moving fluids or granular solids
- G01N21/8507—Probe photometers, i.e. with optical measuring part dipped into fluid sample
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
A fluorometric system for on-line monitoring of biological processes uses a plurality of light sources, each with a different spectral width, to illuminate a sample, the light sources selected to produce emission of fluorescent light, and usually also dispersed light, from the illuminated sample. One of the light sources has a wide spectral range. The light sources are operated sequentially or in combination. Spectra acquired from the emitted light are combined and processed to evaluate properties of the sample, such as concentration of the fluorescing components or particle concentration. The system preferably uses an electro-optical probe in which excitation optical energy is transmitted to the sample directly from the light sources, typically LEDs, without an optical waveguide.
Claims (13)
1. A fluorometric system suitable for monitoring biological processes, the system comprising:
an excitation light source disposed to illuminate a sample to generate emission of light from the sample, the emitted light including fluorescent light, wherein the excitation light source comprises a filter-free broadband light source and at least one monochromatic light source, a light detector for detecting the light emitted from the sample, a processor coupled to the detector for evaluating the sample based on spectral analysis of the emitted light, control means connected operatively with the processor and the excitation light source for activating sequentially or simultaneously the broadband light source and the monochromatic light source or one of the monochromatic light sources, and a probe having one end disposed adjacent to or proximate to the sample, the probe comprising the excitation light source disposed at the one end of the probe, wherein the processor comprises means for evaluating properties of the sample based on combined spectral input produced sequentially or simultaneously by the broadband light source, the monochromatic light source or sources and a combination of the monochromatic light sources.
an excitation light source disposed to illuminate a sample to generate emission of light from the sample, the emitted light including fluorescent light, wherein the excitation light source comprises a filter-free broadband light source and at least one monochromatic light source, a light detector for detecting the light emitted from the sample, a processor coupled to the detector for evaluating the sample based on spectral analysis of the emitted light, control means connected operatively with the processor and the excitation light source for activating sequentially or simultaneously the broadband light source and the monochromatic light source or one of the monochromatic light sources, and a probe having one end disposed adjacent to or proximate to the sample, the probe comprising the excitation light source disposed at the one end of the probe, wherein the processor comprises means for evaluating properties of the sample based on combined spectral input produced sequentially or simultaneously by the broadband light source, the monochromatic light source or sources and a combination of the monochromatic light sources.
2. The system according to Claim 1 wherein the probe comprises a broadband light source and a monochromatic light source, both sources disposed proximate to the sample for transmitting unguided light energy to the sample.
3. The system according to Claim 1 wherein the excitation light source is a set of light emitting diodes.
4. The system according to Claim 1 wherein the probe further comprises an optical fiber disposed to transmit light emitted from the sample to the light detector.
5. The system according to Claim 1, wherein the broadband light source is a UV/VIS light source.
6. The system according to Claim 1, wherein the broadband light source is a plurality of monochromatic light sources.
7. The system according to Claim 1, wherein the at least one monochromatic light source is a light emitting diode or diodes.
8. The system according to claim 7, wherein at least one of the light emitting diodes emits light in an ultraviolet range.
9. The system according to Claim 1, wherein the broadband light source emits light of spectral width from about 200 to about 800 nm.
10. The system according to Claim 1, wherein the excitation light source is in optical communication with the sample through an optical waveguide, each component of the light source being coupled to the waveguide via an optical coupler.
11. A probe for fluorescence measurements of biological samples, the probe comprising an enclosure having an end to be disposed proximate to a sample, a plurality of light sources disposed at the end of the enclosure for unguided transmission of light to the sample, and an optical waveguide having a first end disposed at the end of the enclosure and a second end for transmitting optical signals from the sample to an optical detector.
12. The probe according to Claim 11, wherein the plurality of light sources is arranged substantially concentrically relative to the first end of the optical waveguide.
13. The probe according to Claim 11, wherein the plurality of light sources are light emitting diodes.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11/602,309 US7474400B2 (en) | 2004-07-27 | 2006-11-21 | Multi-wavelength fluorometric system and probe for monitoring of bioprocesses |
| US11/602,309 | 2006-11-21 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2589651A1 true CA2589651A1 (en) | 2008-05-21 |
Family
ID=39420433
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2589651 Abandoned CA2589651A1 (en) | 2006-11-21 | 2007-04-20 | Multi-wavelength fluorometric system and probe for monitoring of bioprocesses |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2589651A1 (en) |
-
2007
- 2007-04-20 CA CA 2589651 patent/CA2589651A1/en not_active Abandoned
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Dead |
Effective date: 20150212 |