CA2584748C - Systeme et procede d'imagerie microscopique multiphotonique fibre d'un echantillon - Google Patents

Systeme et procede d'imagerie microscopique multiphotonique fibre d'un echantillon Download PDF

Info

Publication number
CA2584748C
CA2584748C CA2584748A CA2584748A CA2584748C CA 2584748 C CA2584748 C CA 2584748C CA 2584748 A CA2584748 A CA 2584748A CA 2584748 A CA2584748 A CA 2584748A CA 2584748 C CA2584748 C CA 2584748C
Authority
CA
Canada
Prior art keywords
image guide
laser
sample
excitation
fiber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2584748A
Other languages
English (en)
French (fr)
Other versions
CA2584748A1 (fr
Inventor
Bertrand Viellerobe
Francois Lacombe
Alexandre Loiseau
Frederic Louradour
Mickael Lelek
Alain Barthelemy
Dominique Pagnoux
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Universite de Limoges
Mauna Kea Technologies SA
Original Assignee
Centre National de la Recherche Scientifique CNRS
Universite de Limoges
Mauna Kea Technologies SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Universite de Limoges, Mauna Kea Technologies SA filed Critical Centre National de la Recherche Scientifique CNRS
Publication of CA2584748A1 publication Critical patent/CA2584748A1/fr
Application granted granted Critical
Publication of CA2584748C publication Critical patent/CA2584748C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B23/00—Telescopes, e.g. binoculars; Periscopes; Instruments for viewing the inside of hollow bodies; Viewfinders; Optical aiming or sighting devices
    • G02B23/24—Instruments or systems for viewing the inside of hollow bodies, e.g. fibrescopes
    • G02B23/2476—Non-optical details, e.g. housings, mountings, supports
    • G02B23/2484—Arrangements in relation to a camera or imaging device
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0062—Arrangements for scanning
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
    • A61B5/0084—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters
    • A61B5/0086—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters using infrared radiation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B23/00—Telescopes, e.g. binoculars; Periscopes; Instruments for viewing the inside of hollow bodies; Viewfinders; Optical aiming or sighting devices
    • G02B23/24—Instruments or systems for viewing the inside of hollow bodies, e.g. fibrescopes
    • G02B23/2407—Optical details
    • G02B23/2461—Illumination
    • G02B23/2469—Illumination using optical fibres
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0062—Arrangements for scanning
    • A61B5/0068—Confocal scanning
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0075—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/08—Optical fibres; light guides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/10—Scanning
    • G01N2201/105—Purely optical scan
    • G01N2201/1053—System of scan mirrors for composite motion of beam
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/04—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings formed by bundles of fibres

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Surgery (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Astronomy & Astrophysics (AREA)
  • Radiology & Medical Imaging (AREA)
  • Multimedia (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
  • Endoscopes (AREA)
CA2584748A 2004-10-22 2005-10-21 Systeme et procede d'imagerie microscopique multiphotonique fibre d'un echantillon Expired - Lifetime CA2584748C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0411313 2004-10-22
FR0411313A FR2877103B1 (fr) 2004-10-22 2004-10-22 Systeme et procede d'imagerie microscopique multiphotonique fibre d'un echantillon
PCT/FR2005/002630 WO2006045936A2 (fr) 2004-10-22 2005-10-21 Systeme et procede d’imagerie microscopique multiphotonique fibre d’un echantillon

Publications (2)

Publication Number Publication Date
CA2584748A1 CA2584748A1 (fr) 2006-05-04
CA2584748C true CA2584748C (fr) 2015-10-13

Family

ID=34951919

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2584748A Expired - Lifetime CA2584748C (fr) 2004-10-22 2005-10-21 Systeme et procede d'imagerie microscopique multiphotonique fibre d'un echantillon

Country Status (8)

Country Link
US (1) US8237131B2 (cg-RX-API-DMAC7.html)
EP (1) EP1817620B8 (cg-RX-API-DMAC7.html)
JP (1) JP5102622B2 (cg-RX-API-DMAC7.html)
AU (1) AU2005298494B2 (cg-RX-API-DMAC7.html)
CA (1) CA2584748C (cg-RX-API-DMAC7.html)
ES (1) ES2396426T3 (cg-RX-API-DMAC7.html)
FR (1) FR2877103B1 (cg-RX-API-DMAC7.html)
WO (1) WO2006045936A2 (cg-RX-API-DMAC7.html)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006133509A1 (en) * 2005-06-16 2006-12-21 Swinburne University Of Technology Imaging system
US8554087B2 (en) 2006-09-18 2013-10-08 The Trustees Of Dartmouth College System and method for imaging objects through turbid media
DE102006046925A1 (de) * 2006-09-28 2008-04-03 Jenlab Gmbh Verfahren und Anordnung zur Laser-Endoskopie für die Mikrobearbeitung
JP5307439B2 (ja) * 2007-04-23 2013-10-02 オリンパス株式会社 レーザ顕微鏡
US8059274B2 (en) * 2007-12-07 2011-11-15 The Spectranetics Corporation Low-loss polarized light diversion
DE102009029831A1 (de) 2009-06-17 2011-01-13 W.O.M. World Of Medicine Ag Vorrichtung und Verfahren für die Mehr-Photonen-Fluoreszenzmikroskopie zur Gewinnung von Informationen aus biologischem Gewebe
US8507877B2 (en) * 2009-11-25 2013-08-13 Ofs Fitel, Llc Non-linear optical system and techniques
US9304308B2 (en) * 2009-12-09 2016-04-05 Advanced Micro Devices, Inc. Laser scanning module including an optical isolator
WO2011079126A2 (en) * 2009-12-23 2011-06-30 Laser Biopsy, Inc. Method and apparatus for microscopic imaging system with wide field of view and high collection efficiency
RS61068B1 (sr) * 2010-03-05 2020-12-31 Massachusetts Gen Hospital Uređaj za obezbeđivanje elektro-magnetnog zračenja uzorku
US8792098B2 (en) 2011-06-01 2014-07-29 Digital Light Innovations System and method for hyperspectral illumination
US8891087B2 (en) 2011-06-01 2014-11-18 Digital Light Innovations System and method for hyperspectral imaging
US9356413B2 (en) * 2011-09-30 2016-05-31 Olympus Corporation Laser source apparatus and laser microscope
EP3847968A1 (en) * 2011-11-28 2021-07-14 The Board of Trustees of the Leland Stanford Junior University System and method useful for sarcomere imaging via objective-based microscopy
WO2015108991A2 (en) 2014-01-17 2015-07-23 Imra America, Inc. Laser-based modification of transparent materials
WO2015130651A1 (en) * 2014-02-28 2015-09-03 Imra America, Inc. Multi-wavelength, ultrashort pulse generation and delivery, with applications in microscopy
WO2015163261A1 (ja) * 2014-04-24 2015-10-29 オリンパス株式会社 顕微鏡および顕微鏡観察方法
JP2015227778A (ja) * 2014-05-30 2015-12-17 ソニー株式会社 画像取得装置及び画像取得方法
FR3030956B1 (fr) * 2014-12-18 2018-03-23 Centre National De La Recherche Scientifique - Cnrs Dispositif de transport et de controle d'impulsions lumineuses pour l'imagerie endo-microscopique sans lentille
WO2017046863A1 (ja) 2015-09-15 2017-03-23 オリンパス株式会社 顕微鏡および顕微鏡観察方法
CN109069007A (zh) 2016-03-08 2018-12-21 泽博拉医疗科技公司 皮肤疾病的非侵入式检测
CN109310296A (zh) 2016-04-06 2019-02-05 爱丁堡大学董事会 内窥镜成像装置及方法
US9645291B1 (en) * 2016-04-18 2017-05-09 Ii-Vi Incorporated Voltage-tunable optical filters for instrumentation applications
EP3614915A4 (en) 2017-04-28 2021-01-20 Enspectra Health, Inc. SARCOMAS IMAGING AND MEASUREMENT SYSTEMS AND METHODS
GB201707239D0 (en) 2017-05-05 2017-06-21 Univ Edinburgh Optical system and method
WO2020010330A1 (en) * 2018-07-06 2020-01-09 The Trustees Of Columbia University In The City Of New York Collagen tissue modification
AU2019381715B2 (en) 2018-11-13 2025-06-26 Enspectra Health, Inc. Methods and systems for generating depth profiles
CN109656014B (zh) * 2019-01-31 2024-03-19 北京超维景生物科技有限公司 多路荧光收集装置及三维非线性激光扫描腔体内窥镜
CN113349708B (zh) * 2021-06-08 2023-05-26 之江实验室 一种基于大色散镜头的双模态高分辨大深度显微内窥成像系统
KR102567260B1 (ko) * 2021-09-01 2023-08-16 연세대학교 산학협력단 망막 진단 시스템 및 이를 이용한 망막 진단방법
CN114755210A (zh) * 2022-04-22 2022-07-15 波粒光子(苏州)精密光电科技有限公司 高性能光纤荧光探测器及其生产方法
CN119523389B (zh) * 2024-11-20 2025-09-23 卓外（上海）医疗电子科技有限公司 一种基于谐波成像和多光子荧光成像的内窥镜系统

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4655547A (en) * 1985-04-09 1987-04-07 Bell Communications Research, Inc. Shaping optical pulses by amplitude and phase masking
JP3269713B2 (ja) * 1993-09-03 2002-04-02 株式会社日立製作所 光伝送システム
DE19622359B4 (de) * 1996-06-04 2007-11-22 Carl Zeiss Jena Gmbh Vorrichtung zur Einkopplung der Strahlung von Kurzpulslasern in einem mikroskopischen Strahlengang
US6249630B1 (en) * 1996-12-13 2001-06-19 Imra America, Inc. Apparatus and method for delivery of dispersion-compensated ultrashort optical pulses with high peak power
US5862287A (en) * 1996-12-13 1999-01-19 Imra America, Inc. Apparatus and method for delivery of dispersion compensated ultrashort optical pulses with high peak power
US5995281A (en) * 1997-04-09 1999-11-30 Carl Zeiss Jena Gmbh Device for coupling the radiation of short-pulse lasers in an optical beam path of a microscope
DE19927724A1 (de) 1998-06-19 2000-01-20 Optiscan Pty Ltd Verfahren und Vorrichtung mit Zweiphotonen-Endoskop oder Mikroskop
US6449039B1 (en) * 1999-07-28 2002-09-10 Thermo Noran Inc. Laser scanning fluorescence microscopy with compensation for spatial dispersion of fast laser pulses
US6369928B1 (en) * 2000-11-01 2002-04-09 Optical Biopsy Technologies, Inc. Fiber-coupled, angled-dual-illumination-axis confocal scanning microscopes for performing reflective and two-photon fluorescence imaging
JP2003344777A (ja) * 2002-05-24 2003-12-03 Japan Science & Technology Corp 光ファイバ顕微鏡および内視鏡
FR2842407B1 (fr) * 2002-07-18 2005-05-06 Mauna Kea Technologies "procede et appareillage d'imagerie de fluorescence confocale fibree"
FR2842628B1 (fr) 2002-07-18 2004-09-24 Mauna Kea Technologies "procede de traitement d'une image acquise au moyen d'un guide compose d'une pluralite de fibres optiques"

Also Published As

Publication number Publication date
US8237131B2 (en) 2012-08-07
AU2005298494A1 (en) 2006-05-04
ES2396426T3 (es) 2013-02-21
AU2005298494B2 (en) 2011-08-11
WO2006045936A3 (fr) 2006-06-22
JP2008517291A (ja) 2008-05-22
EP1817620B1 (fr) 2012-09-26
EP1817620A2 (fr) 2007-08-15
FR2877103B1 (fr) 2012-02-10
WO2006045936A2 (fr) 2006-05-04
JP5102622B2 (ja) 2012-12-19
CA2584748A1 (fr) 2006-05-04
EP1817620B8 (fr) 2012-12-12
FR2877103A1 (fr) 2006-04-28
US20070290145A1 (en) 2007-12-20

Similar Documents

Publication Publication Date Title
CA2584748C (fr) Systeme et procede d'imagerie microscopique multiphotonique fibre d'un echantillon
CA2491748C (fr) Procede et appareillage d'imagerie de fluorescence haute resolution par fibre optique et notamment d'imagerie confocale
CA2344165C (fr) Dispositif d'observation de l'interieur d'un corps produisant une qualite d'observation perfectionnee
EP1766373B1 (fr) Procede et systeme d'imagerie microscopique de fluorescence fibree multimarquage
EP1468322B1 (fr) Appareillage d imagerie confocale notamment pour endoscope
EP1461601B1 (fr) Appareillage de spectroscopie d'autofluorescence subsurfacique
EP2005143B1 (fr) Microscopie de fluorescence fibree a base de bleu de methylene
EP2024774B1 (fr) Dispositif et procede d'endoscopie pour une observation simultanee de plusieurs zones d'interet

Legal Events

Date Code Title Description
EEER Examination request