CA2538085A1 - Synthetic antimicrobial polypeptides - Google Patents

Synthetic antimicrobial polypeptides Download PDF

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Publication number
CA2538085A1
CA2538085A1 CA002538085A CA2538085A CA2538085A1 CA 2538085 A1 CA2538085 A1 CA 2538085A1 CA 002538085 A CA002538085 A CA 002538085A CA 2538085 A CA2538085 A CA 2538085A CA 2538085 A1 CA2538085 A1 CA 2538085A1
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Canada
Prior art keywords
polypeptide
antimicrobial
amino acid
group
amino acids
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Granted
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CA002538085A
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French (fr)
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CA2538085C (en
Inventor
Hans-Henrik Kristensen Hoegenhaug
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Novozymes Biopharma DK AS
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Individual
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4723—Cationic antimicrobial peptides, e.g. defensins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/02—Local antiseptics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A61P31/06—Antibacterial agents for tuberculosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
    • A61P33/06—Antimalarials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Pulmonology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cosmetics (AREA)
  • Detergent Compositions (AREA)

Abstract

The present invention relates to polypeptides having antimicrobial activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.

Claims (27)

1. A polypeptide having antimicrobial activity, comprising the amino acid sequence:
X1-X2-X3-X4-X5;
wherein each of X1, X2, X3, X4 and X5 is selected from the group of amino acid sequences:
P-P-R-F, P-R-F-P, R-F-P-P, F-P-P-R, P-R-P-F, R-P-F-P, P-F-P-R, F-P-R-P, P-P-F-R, P-F-R-P, F-R-P-P, R-P-P-F, P-P-R-L, P-R-L-P, R-L-P-P, L-P-P-R, P-R-P-L, R-P-L-P, P-L-P-R, L-P-R-P, P-P-L-R, P-L-R-P, L-R-P-P, R-P-P-L, P-P-R-V, P-R-V-P, R-V-P-P, V-P-P-R, P-R-P-V, R-P-V-P, P-V-P-R, V-P-R-P, P-P-V-R, P-V-R-P, V-R-P-P, R-P-P-V, P-P-R-I, P-R-I-P, R-I-P-P, I-P-P-R, P-R-P-I, R-P-I-P, P-I-P-R, I-P-R-P, P-P-I-R, P-I-R-P, I-R-P-P and R-P-P-I;
and wherein at the most four of X1 - X5 are identical.
2. The polypeptide of claim 1, wherein X1, X2, X3, X4 and X5 are selected from the group of amino acid sequences:
P-P-R-Z1, P-R-Z2-P, R-Z3-P-P, Z4-P-P-R, P-R-P-Z5, R-P-Z6-P, P-Z7-P-R, Z8-P-R-P, P-P-Z9-R, P-Z10-R-P, Z11-R-P-P and R-P-P-Z12;
wherein X1 - X5 are identical, except for the Z1 - Z12 amino acids; and where in Z1 - Z12 are selected from the group of amino acids consisting of F, L, V and I.
3. The polypeptide of claim 1, comprising the amino acid sequence:
X1-X2-X3-X4-X5-X6, wherein each of X1, X2, X3, X4, X5 and X6 is selected from the group of amino acid sequences:
P-P-R-F, P-R-F-P, R-F-P-P, F-P-P-R, P-R-P-F, R-P-F-P, P-F-P-R, F-P-R-P, P-P-F-R, P-F-R-P, F-R-P-P, R-P-P-F, P-P-R-L, P-R-L-P, R-L-P-P, L-P-P-R, P-R-P-L, R-P-L-P, P-L-P-R, L-P-R-P, P-P-L-R, P-L-R-P, L-R-P-P, R-P-P-L, P-P-R-V, P-R-V-P, R-V-P-P, V-P-P-R, P-R-P-V, R-P-V-P, P-V-P-R, V-P-R-P, P-P-V-R, P-V-R-P, V-R-P-P, R-P-P-V, P-P-R-I, P-R-I-P, R-I-P-P, I-P-P-R, P-R-P-I, R-P-I-P, P-I-P-R, I-P-R-P, P-P-I-R, P-I-R-P, I-R-P-P and R-P-P-I;
and wherein at the most four of X1 - X6 are identical.
4. The polypeptide of claim 3, wherein X1, X2, X3, X4, X5 and X6 are selected from the group of amino acid sequences:
P-P-R-Z1, P-R-Z2-P, R-Z3-P-P, Z4-P-P-R, P-R-P-Z5, R-P-Z6-P, P-Z7-P-R, Z8-P-R-P, P-P-Z9-R, P-Z10-R-P, Z11-R-P-P and R-P-P-Z12;
wherein X1 - X6 are identical, except for the Z1 - Z12 amino acids; and where in Z1 - Z12 are selected from the group of amino acids consisting of F, L, V and I.
5. The polypeptide of anyone of claims 1-4, wherein at the most three of X1 -X5 are identical.
6. The polypeptide of anyone of claims 1-5, wherein at the most two of X1 - X5 are identical.
7. The polypeptide of anyone of claims 1-6, wherein X1-X2-X3-X4-X5 or X1-X2-X3-X4-X5-X6 is directly preceded by an amino acid sequence of from 3 to 10 amino acids of which at least two amino acids are positively charged at neutral pH.
8. The polypeptide of anyone of claims 1-6, which consists of the amino acid sequence:
X1-X2-X3-X4-X5 or X1-X2-X3-X4-X5-X6.
9. A polynucleotide having a nucleotide sequence which encodes for the polypeptide defined in anyone of claims 1-8.
10. A nucleic acid construct comprising the nucleotide sequence defined in claim 9 operably linked to one or more control sequences that direct the production of the polypeptide in a suitable host.
11. A recombinant expression vector comprising the nucleic acid construct defined in claim 10.
12. A recombinant host cell comprising the nucleic acid construct defined in claim 10.
13. A method for producing a polypeptide as defined in any of claims 1-8, the method comprising:
(a) cultivating a recombinant host cell as defined in claim 12 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
14. A composition comprising an antimicrobial polypeptide as defined in any of claims 1-8.
15. The composition of claim 14, which further comprises an additional biocidal agent.
16. A method for killing or inhibiting growth of microbial cells comprising contacting the microbial cells with an antimicrobial polypeptide as defined in any of claims 1-8.
17. A detergent composition comprising a surfactant and an antimicrobial polypeptide as defined in any of claims 1-8.
18. An antimicrobial polypeptide as defined in any of claims 1-8 for use as a medicament.
19. An antimicrobial polypeptide as defined in any of claims 1-8 for use as an antimicrobial veterinarian or human therapeutic or prophylactic agent.
20. Use of an antimicrobial polypeptide as defined in any of claims 1-8 for use in the preparation of a veterinarian or human therapeutic agent for the treatment of a microbial infection or for prophylactic use.
21. Use of an antimicrobial polypeptide as defined in any of claims 1-8 for killing or inhibiting growth of microbial cells.
22. A transgenic plant, plant part or plant cell, which has been transformed with a nucleotide sequence encoding a polypeptide having antimicrobial activity as defined in any of claims 1-8.
23. Use of at least one antimicrobial polypeptide as defined in any of claims 1-8 in animal feed.
24. Use of at least one antimicrobial polypeptide as defined in any of claims 1-8 in the preparation of a composition for use in animal feed.
25. An animal feed additive comprising (a) at least one antimicrobial polypeptide as defined in any of claims 1-8;
and (b) at least one fat soluble vitamin, and/or (c) at least one water soluble vitamin, and/or (d) at least one trace mineral, and/or (e) at least one macro mineral.
26. The animal feed additive of claim 25, which further comprises phytase, xylanase, galactanase, and/or beta-glucanase.
27. An animal feed composition having a crude protein content of 50 to 800 g/kg and comprising at least one antimicrobial polypeptide as defined in any of claims 1-8.
CA2538085A 2003-09-12 2004-09-13 Synthetic antimicrobial polypeptides Expired - Fee Related CA2538085C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200301324 2003-09-12
DKPA200301324 2003-09-12
PCT/DK2004/000606 WO2005026204A2 (en) 2003-09-12 2004-09-13 Synthetic antimicrobial polypeptides

Publications (2)

Publication Number Publication Date
CA2538085A1 true CA2538085A1 (en) 2005-03-24
CA2538085C CA2538085C (en) 2011-03-15

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CA2538085A Expired - Fee Related CA2538085C (en) 2003-09-12 2004-09-13 Synthetic antimicrobial polypeptides

Country Status (10)

Country Link
EP (1) EP1664107A2 (en)
JP (1) JP2007527228A (en)
CN (2) CN101113173A (en)
AU (1) AU2004272170B2 (en)
BR (1) BRPI0414253A (en)
CA (1) CA2538085C (en)
IL (1) IL174092A (en)
NZ (1) NZ545815A (en)
WO (1) WO2005026204A2 (en)
ZA (1) ZA200601999B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO20041892D0 (en) * 2004-05-07 2004-05-07 Uni I Tromso Novel crustacean proline-rich peptides
US8124721B2 (en) 2005-10-04 2012-02-28 Inimex Pharmaceuticals Inc. Peptides for modulating innate immunity
JP2012100639A (en) * 2010-11-09 2012-05-31 Kai:Kk Method of washing, application and antibacterial treatment using enzyme treatment solution

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE468516B (en) * 1991-06-14 1993-02-01 Kabigen Ab NEW POLYPEPTIDES WHICH CAN BE OBTAINED FROM THE TIN GAS AND THEIR USE
EP0665239A1 (en) * 1994-01-26 1995-08-02 Consiglio Nazionale Delle Ricerche Peptide derivatives of bactenecin endowed with antibacterial activity
US5830993A (en) * 1995-04-10 1998-11-03 Kansas State University Research Foundation Synthetic antimicrobial peptide

Also Published As

Publication number Publication date
CN1878791A (en) 2006-12-13
WO2005026204A3 (en) 2005-04-28
JP2007527228A (en) 2007-09-27
CA2538085C (en) 2011-03-15
NZ545815A (en) 2009-11-27
AU2004272170A1 (en) 2005-03-24
WO2005026204A2 (en) 2005-03-24
CN101113173A (en) 2008-01-30
EP1664107A2 (en) 2006-06-07
AU2004272170B2 (en) 2011-04-07
BRPI0414253A (en) 2006-11-21
IL174092A (en) 2010-11-30
ZA200601999B (en) 2007-02-28
IL174092A0 (en) 2006-08-01

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