CA2537326A1 - C1q related protein - Google Patents

Info

Publication number

CA2537326A1

CA2537326A1 CA002537326A CA2537326A CA2537326A1 CA 2537326 A1 CA2537326 A1 CA 2537326A1 CA 002537326 A CA002537326 A CA 002537326A CA 2537326 A CA2537326 A CA 2537326A CA 2537326 A1 CA2537326 A1 CA 2537326A1

Authority

CA

Canada

Prior art keywords

polypeptide

seq

nucleic acid

disease

acid molecule

Prior art date

2003-10-01

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Abandoned

Links

• Espacenet
• Global Dossier
• CIPO
• Discuss

• 108090000623 proteins and genes Proteins 0.000 title claims abstract description 166
• 102000004169 proteins and genes Human genes 0.000 title claims abstract description 100
• 150000007523 nucleic acids Chemical group 0.000 claims abstract description 136
• 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 102
• 201000010099 disease Diseases 0.000 claims abstract description 93
• 238000011282 treatment Methods 0.000 claims abstract description 16
• 238000003745 diagnosis Methods 0.000 claims abstract description 13
• 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 12
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 414
• 229920001184 polypeptide Polymers 0.000 claims description 406
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 406
• 238000000034 method Methods 0.000 claims description 146
• 102000039446 nucleic acids Human genes 0.000 claims description 127
• 108020004707 nucleic acids Proteins 0.000 claims description 127
• 230000014509 gene expression Effects 0.000 claims description 76
• 150000001875 compounds Chemical class 0.000 claims description 71
• 239000003446 ligand Substances 0.000 claims description 50
• 239000013598 vector Substances 0.000 claims description 49
• 239000000203 mixture Substances 0.000 claims description 40
• 230000000694 effects Effects 0.000 claims description 39
• HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims description 39
• 239000000523 sample Substances 0.000 claims description 39
• 241000282414 Homo sapiens Species 0.000 claims description 35
• 239000012634 fragment Substances 0.000 claims description 35
• 238000009739 binding Methods 0.000 claims description 34
• 230000027455 binding Effects 0.000 claims description 33
• 108010035532 Collagen Proteins 0.000 claims description 30
• 102000008186 Collagen Human genes 0.000 claims description 30
• 229920001436 collagen Polymers 0.000 claims description 30
• 230000035772 mutation Effects 0.000 claims description 22
• 230000006870 function Effects 0.000 claims description 21
• 210000001519 tissue Anatomy 0.000 claims description 21
• 108020001507 fusion proteins Proteins 0.000 claims description 20
• 102000037865 fusion proteins Human genes 0.000 claims description 20
• 230000001225 therapeutic effect Effects 0.000 claims description 19
• 239000005557 antagonist Substances 0.000 claims description 18
• 238000004519 manufacturing process Methods 0.000 claims description 17
• 239000000758 substrate Substances 0.000 claims description 17
• 102000004190 Enzymes Human genes 0.000 claims description 16
• 108090000790 Enzymes Proteins 0.000 claims description 16
• 239000000556 agonist Substances 0.000 claims description 16
• 230000015572 biosynthetic process Effects 0.000 claims description 16
• 238000001514 detection method Methods 0.000 claims description 16
• 208000015181 infectious disease Diseases 0.000 claims description 16
• 108010076504 Protein Sorting Signals Proteins 0.000 claims description 14
• 125000003275 alpha amino acid group Chemical group 0.000 claims description 14
• 108020003175 receptors Proteins 0.000 claims description 14
• 102000005962 receptors Human genes 0.000 claims description 14
• 208000033051 Fuchs endothelial corneal dystrophy Diseases 0.000 claims description 12
• 241001465754 Metazoa Species 0.000 claims description 12
• 125000000539 amino acid group Chemical group 0.000 claims description 12
• 239000008194 pharmaceutical composition Substances 0.000 claims description 12
• 208000001517 late-onset retinal degeneration Diseases 0.000 claims description 11
• 238000012216 screening Methods 0.000 claims description 11
• 208000008589 Obesity Diseases 0.000 claims description 10
• 206010064930 age-related macular degeneration Diseases 0.000 claims description 10
• 239000003153 chemical reaction reagent Substances 0.000 claims description 10
• 239000003795 chemical substances by application Substances 0.000 claims description 10
• 239000003814 drug Substances 0.000 claims description 10
• 208000002780 macular degeneration Diseases 0.000 claims description 10
• 235000020824 obesity Nutrition 0.000 claims description 10
• 229960005486 vaccine Drugs 0.000 claims description 10
• 206010011878 Deafness Diseases 0.000 claims description 9
• 206010061218 Inflammation Diseases 0.000 claims description 9
• 210000000988 bone and bone Anatomy 0.000 claims description 9
• 230000004054 inflammatory process Effects 0.000 claims description 9
• 208000002197 Ehlers-Danlos syndrome Diseases 0.000 claims description 8
• 206010028980 Neoplasm Diseases 0.000 claims description 8
• 108020004711 Nucleic Acid Probes Proteins 0.000 claims description 8
• 208000016354 hearing loss disease Diseases 0.000 claims description 8
• 239000002853 nucleic acid probe Substances 0.000 claims description 8
• 201000001320 Atherosclerosis Diseases 0.000 claims description 7
• 208000023275 Autoimmune disease Diseases 0.000 claims description 7
• 206010018364 Glomerulonephritis Diseases 0.000 claims description 7
• 230000000890 antigenic effect Effects 0.000 claims description 7
• 208000035143 Bacterial infection Diseases 0.000 claims description 6
• 102100036217 Collagen alpha-1(X) chain Human genes 0.000 claims description 6
• 201000010806 Schmid metaphyseal chondrodysplasia Diseases 0.000 claims description 6
• 239000013068 control sample Substances 0.000 claims description 6
• 206010012601 diabetes mellitus Diseases 0.000 claims description 6
• 230000009261 transgenic effect Effects 0.000 claims description 6
• 201000004569 Blindness Diseases 0.000 claims description 5
• 206010012289 Dementia Diseases 0.000 claims description 5
• 206010051066 Gastrointestinal stromal tumour Diseases 0.000 claims description 5
• 208000007569 Giant Cell Tumors Diseases 0.000 claims description 5
• 101000854936 Homo sapiens Visual system homeobox 1 Proteins 0.000 claims description 5
• 208000014042 Spondylometaphyseal dysplasia Diseases 0.000 claims description 5
• 102100020673 Visual system homeobox 1 Human genes 0.000 claims description 5
• 206010047623 Vitamin C deficiency Diseases 0.000 claims description 5
• 206010003246 arthritis Diseases 0.000 claims description 5
• 230000004071 biological effect Effects 0.000 claims description 5
• 201000005217 chondroblastoma Diseases 0.000 claims description 5
• 201000004180 corneal endothelial dystrophy Diseases 0.000 claims description 5
• 201000011243 gastrointestinal stromal tumor Diseases 0.000 claims description 5
• 230000001939 inductive effect Effects 0.000 claims description 5
• 201000008968 osteosarcoma Diseases 0.000 claims description 5
• 201000004207 posterior polymorphous corneal dystrophy Diseases 0.000 claims description 5
• 208000010233 scurvy Diseases 0.000 claims description 5
• 208000024827 Alzheimer disease Diseases 0.000 claims description 4
• 241000598436 Human T-cell lymphotropic virus Species 0.000 claims description 4
• 206010023563 Labyrinthine fistula Diseases 0.000 claims description 4
• 206010023567 Labyrinthitis Diseases 0.000 claims description 4
• 206010031243 Osteogenesis imperfecta Diseases 0.000 claims description 4
• 201000010810 Otospondylomegaepiphyseal dysplasia Diseases 0.000 claims description 4
• 208000018737 Parkinson disease Diseases 0.000 claims description 4
• 208000009205 Tinnitus Diseases 0.000 claims description 4
• 208000036142 Viral infection Diseases 0.000 claims description 4
• 201000000021 Weissenbacher-Zweymuller syndrome Diseases 0.000 claims description 4
• 206010002022 amyloidosis Diseases 0.000 claims description 4
• 208000007474 aortic aneurysm Diseases 0.000 claims description 4
• 210000001367 artery Anatomy 0.000 claims description 4
• 208000027625 autoimmune inner ear disease Diseases 0.000 claims description 4
• 208000036351 autosomal dominant otospondylomegaepiphyseal dysplasia Diseases 0.000 claims description 4
• 208000022362 bacterial infectious disease Diseases 0.000 claims description 4
• 239000012472 biological sample Substances 0.000 claims description 4
• 201000011510 cancer Diseases 0.000 claims description 4
• 231100000895 deafness Toxicity 0.000 claims description 4
• 238000002224 dissection Methods 0.000 claims description 4
• 230000010370 hearing loss Effects 0.000 claims description 4
• 231100000888 hearing loss Toxicity 0.000 claims description 4
• 230000000813 microbial effect Effects 0.000 claims description 4
• 238000012544 monitoring process Methods 0.000 claims description 4
• 230000004770 neurodegeneration Effects 0.000 claims description 4
• 208000015122 neurodegenerative disease Diseases 0.000 claims description 4
• 238000002560 therapeutic procedure Methods 0.000 claims description 4
• 231100000886 tinnitus Toxicity 0.000 claims description 4
• 230000009385 viral infection Effects 0.000 claims description 4
• 239000013610 patient sample Substances 0.000 claims description 3
• 208000011580 syndromic disease Diseases 0.000 claims description 3
• 230000007423 decrease Effects 0.000 claims description 2
• 238000000338 in vitro Methods 0.000 claims description 2
• 208000030852 Parasitic disease Diseases 0.000 claims 2
• 230000002265 prevention Effects 0.000 abstract description 2
• 210000004027 cell Anatomy 0.000 description 105
• 235000018102 proteins Nutrition 0.000 description 90
• 108020004414 DNA Proteins 0.000 description 60
• 238000003752 polymerase chain reaction Methods 0.000 description 53
• 235000014304 histidine Nutrition 0.000 description 33
• 239000000047 product Substances 0.000 description 26
• 238000000746 purification Methods 0.000 description 25
• 239000000872 buffer Substances 0.000 description 24
• 239000013612 plasmid Substances 0.000 description 23
• 235000001014 amino acid Nutrition 0.000 description 21
• 238000003556 assay Methods 0.000 description 21
• 238000006243 chemical reaction Methods 0.000 description 21
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 20
• 150000001413 amino acids Chemical class 0.000 description 19
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 18
• 238000003776 cleavage reaction Methods 0.000 description 17
• 230000007017 scission Effects 0.000 description 17
• 230000004048 modification Effects 0.000 description 16
• 238000012986 modification Methods 0.000 description 16
• 230000001105 regulatory effect Effects 0.000 description 16
• 238000010367 cloning Methods 0.000 description 15
• 230000000295 complement effect Effects 0.000 description 15
• 125000003729 nucleotide group Chemical group 0.000 description 15
• 108091026890 Coding region Proteins 0.000 description 14
• 238000009396 hybridization Methods 0.000 description 14
• 239000012528 membrane Substances 0.000 description 14
• 239000002773 nucleotide Substances 0.000 description 14
• 102100031786 Adiponectin Human genes 0.000 description 13
• 210000003050 axon Anatomy 0.000 description 13
• 239000002299 complementary DNA Substances 0.000 description 13
• 230000002068 genetic effect Effects 0.000 description 13
• 210000004379 membrane Anatomy 0.000 description 13
• 108020004999 messenger RNA Proteins 0.000 description 13
• 241000196324 Embryophyta Species 0.000 description 12
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
• 241000700605 Viruses Species 0.000 description 12
• 238000013459 approach Methods 0.000 description 12
• 239000013604 expression vector Substances 0.000 description 12
• 238000005755 formation reaction Methods 0.000 description 12
• RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 12
• 239000007787 solid Substances 0.000 description 12
• 238000012360 testing method Methods 0.000 description 12
• 108010076365 Adiponectin Proteins 0.000 description 11
• 108091034117 Oligonucleotide Proteins 0.000 description 11
• 238000005516 engineering process Methods 0.000 description 11
• 210000000170 cell membrane Anatomy 0.000 description 10
• 238000012545 processing Methods 0.000 description 10
• 238000012163 sequencing technique Methods 0.000 description 10
• 239000000243 solution Substances 0.000 description 10
• 230000003321 amplification Effects 0.000 description 9
• 230000000692 anti-sense effect Effects 0.000 description 9
• 230000001640 apoptogenic effect Effects 0.000 description 9
• 210000004899 c-terminal region Anatomy 0.000 description 9
• 208000035475 disorder Diseases 0.000 description 9
• 239000003550 marker Substances 0.000 description 9
• 238000003199 nucleic acid amplification method Methods 0.000 description 9
• 230000008569 process Effects 0.000 description 9
• 238000001712 DNA sequencing Methods 0.000 description 8
• 102100040247 Tumor necrosis factor Human genes 0.000 description 8
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 8
• 230000004913 activation Effects 0.000 description 8
• 239000003937 drug carrier Substances 0.000 description 8
• BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 8
• 239000002243 precursor Substances 0.000 description 8
• 238000013519 translation Methods 0.000 description 8
• 102100034028 Membrane-bound transcription factor site-1 protease Human genes 0.000 description 7
• 238000007792 addition Methods 0.000 description 7
• 238000004458 analytical method Methods 0.000 description 7
• 230000001580 bacterial effect Effects 0.000 description 7
• 230000002759 chromosomal effect Effects 0.000 description 7
• 239000000499 gel Substances 0.000 description 7
• 238000003780 insertion Methods 0.000 description 7
• 230000037431 insertion Effects 0.000 description 7
• -1 polyethylene Polymers 0.000 description 7
• 230000004044 response Effects 0.000 description 7
• 239000000126 substance Substances 0.000 description 7
• 238000006467 substitution reaction Methods 0.000 description 7
• 238000013518 transcription Methods 0.000 description 7
• 230000035897 transcription Effects 0.000 description 7
• 230000003612 virological effect Effects 0.000 description 7
• QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
• 108090000994 Catalytic RNA Proteins 0.000 description 6
• 102000053642 Catalytic RNA Human genes 0.000 description 6
• 102100040496 Collagen alpha-2(VIII) chain Human genes 0.000 description 6
• 108020004635 Complementary DNA Proteins 0.000 description 6
• 108700024394 Exon Proteins 0.000 description 6
• 101000749886 Homo sapiens Collagen alpha-2(VIII) chain Proteins 0.000 description 6
• OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
• 238000004113 cell culture Methods 0.000 description 6
• 230000003247 decreasing effect Effects 0.000 description 6
• 238000012217 deletion Methods 0.000 description 6
• 230000037430 deletion Effects 0.000 description 6
• 238000001415 gene therapy Methods 0.000 description 6
• NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 6
• 210000001265 otolithic membrane Anatomy 0.000 description 6
• 238000003127 radioimmunoassay Methods 0.000 description 6
• 238000011160 research Methods 0.000 description 6
• 108091092562 ribozyme Proteins 0.000 description 6
• 238000001890 transfection Methods 0.000 description 6
• 108010022510 Collagen Type X Proteins 0.000 description 5
• 102000030746 Collagen Type X Human genes 0.000 description 5
• 238000002965 ELISA Methods 0.000 description 5
• 102100038132 Endogenous retrovirus group K member 6 Pro protein Human genes 0.000 description 5
• 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 5
• 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 5
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
• 101710172711 Structural protein Proteins 0.000 description 5
• 108060008682 Tumor Necrosis Factor Proteins 0.000 description 5
• 230000002159 abnormal effect Effects 0.000 description 5
• 238000001042 affinity chromatography Methods 0.000 description 5
• 210000001124 body fluid Anatomy 0.000 description 5
• 239000000969 carrier Substances 0.000 description 5
• 230000007547 defect Effects 0.000 description 5
• 210000003027 ear inner Anatomy 0.000 description 5
• 239000002158 endotoxin Substances 0.000 description 5
• 238000011534 incubation Methods 0.000 description 5
• 238000002347 injection Methods 0.000 description 5
• 239000007924 injection Substances 0.000 description 5
• 230000003993 interaction Effects 0.000 description 5
• 238000012423 maintenance Methods 0.000 description 5
• 210000004962 mammalian cell Anatomy 0.000 description 5
• 230000001404 mediated effect Effects 0.000 description 5
• 239000002609 medium Substances 0.000 description 5
• 229910052751 metal Inorganic materials 0.000 description 5
• 239000002184 metal Substances 0.000 description 5
• 230000001575 pathological effect Effects 0.000 description 5
• 239000008223 sterile water Substances 0.000 description 5
• 208000001072 type 2 diabetes mellitus Diseases 0.000 description 5
• 102100024337 Collagen alpha-1(VIII) chain Human genes 0.000 description 4
• 102000053602 DNA Human genes 0.000 description 4
• 108090001126 Furin Proteins 0.000 description 4
• 241000238631 Hexapoda Species 0.000 description 4
• 241000282412 Homo Species 0.000 description 4
• 101000909492 Homo sapiens Collagen alpha-1(VIII) chain Proteins 0.000 description 4
• 208000026350 Inborn Genetic disease Diseases 0.000 description 4
• 206010022489 Insulin Resistance Diseases 0.000 description 4
• 101710193467 Membrane-bound transcription factor site-1 protease Proteins 0.000 description 4
• 208000027530 Meniere disease Diseases 0.000 description 4
• 208000012902 Nervous system disease Diseases 0.000 description 4
• 241000356847 Otolithes Species 0.000 description 4
• 238000012408 PCR amplification Methods 0.000 description 4
• 108091005804 Peptidases Proteins 0.000 description 4
• 108091093037 Peptide nucleic acid Proteins 0.000 description 4
• 102000006437 Proprotein Convertases Human genes 0.000 description 4
• 108010044159 Proprotein Convertases Proteins 0.000 description 4
• 102100038946 Proprotein convertase subtilisin/kexin type 6 Human genes 0.000 description 4
• 239000004365 Protease Substances 0.000 description 4
• 102000006382 Ribonucleases Human genes 0.000 description 4
• 108010083644 Ribonucleases Proteins 0.000 description 4
• 206010040070 Septic Shock Diseases 0.000 description 4
• 241000282485 Vulpes vulpes Species 0.000 description 4
• 239000011543 agarose gel Substances 0.000 description 4
• 239000010839 body fluid Substances 0.000 description 4
• 230000001413 cellular effect Effects 0.000 description 4
• 238000004587 chromatography analysis Methods 0.000 description 4
• 230000002950 deficient Effects 0.000 description 4
• 238000009472 formulation Methods 0.000 description 4
• 230000004927 fusion Effects 0.000 description 4
• 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 4
• 230000002163 immunogen Effects 0.000 description 4
• 239000003112 inhibitor Substances 0.000 description 4
• 230000005764 inhibitory process Effects 0.000 description 4
• 238000002955 isolation Methods 0.000 description 4
• 229920006008 lipopolysaccharide Polymers 0.000 description 4
• 239000007788 liquid Substances 0.000 description 4
• 239000000463 material Substances 0.000 description 4
• 239000011159 matrix material Substances 0.000 description 4
• 210000003470 mitochondria Anatomy 0.000 description 4
• 230000037361 pathway Effects 0.000 description 4
• 229910052697 platinum Inorganic materials 0.000 description 4
• 230000002441 reversible effect Effects 0.000 description 4
• 238000012552 review Methods 0.000 description 4
• 230000003248 secreting effect Effects 0.000 description 4
• 210000004739 secretory vesicle Anatomy 0.000 description 4
• 239000000725 suspension Substances 0.000 description 4
• 241000701447 unidentified baculovirus Species 0.000 description 4
• 238000001262 western blot Methods 0.000 description 4
• 102000040650 (ribonucleotides)n+m Human genes 0.000 description 3
• 241000894006 Bacteria Species 0.000 description 3
• 108010074051 C-Reactive Protein Proteins 0.000 description 3
• 102100032752 C-reactive protein Human genes 0.000 description 3
• 108010069526 Collagen Type VIII Proteins 0.000 description 3
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
• 229920002307 Dextran Polymers 0.000 description 3
• 238000009007 Diagnostic Kit Methods 0.000 description 3
• 108010013369 Enteropeptidase Proteins 0.000 description 3
• 102100029727 Enteropeptidase Human genes 0.000 description 3
• 241000588724 Escherichia coli Species 0.000 description 3
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
• 101100017008 Homo sapiens HHAT gene Proteins 0.000 description 3
• 101001098833 Homo sapiens Proprotein convertase subtilisin/kexin type 6 Proteins 0.000 description 3
• 108060003951 Immunoglobulin Proteins 0.000 description 3
• 102000004877 Insulin Human genes 0.000 description 3
• 108090001061 Insulin Proteins 0.000 description 3
• 108060001084 Luciferase Proteins 0.000 description 3
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 3
• 101150084626 Mbtps1 gene Proteins 0.000 description 3
• 102100023354 Multimerin-1 Human genes 0.000 description 3
• 241000699666 Mus <mouse, genus> Species 0.000 description 3
• 102100037732 Neuroendocrine convertase 2 Human genes 0.000 description 3
• 241000283973 Oryctolagus cuniculus Species 0.000 description 3
• 102100034208 Otolin-1 Human genes 0.000 description 3
• 108010071690 Prealbumin Proteins 0.000 description 3
• 102000007584 Prealbumin Human genes 0.000 description 3
• 102100038950 Proprotein convertase subtilisin/kexin type 7 Human genes 0.000 description 3
• 101710180647 Proprotein convertase subtilisin/kexin type 7 Proteins 0.000 description 3
• 101710118538 Protease Proteins 0.000 description 3
• 108010029485 Protein Isoforms Proteins 0.000 description 3
• 102000001708 Protein Isoforms Human genes 0.000 description 3
• 101100264226 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) XRN1 gene Proteins 0.000 description 3
• 229920005654 Sephadex Polymers 0.000 description 3
• 239000012507 Sephadex™ Substances 0.000 description 3
• 238000012300 Sequence Analysis Methods 0.000 description 3
• MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 3
• 241000251539 Vertebrata <Metazoa> Species 0.000 description 3
• 230000004075 alteration Effects 0.000 description 3
• 229960000723 ampicillin Drugs 0.000 description 3
• AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
• 238000010171 animal model Methods 0.000 description 3
• 239000000427 antigen Substances 0.000 description 3
• 108091007433 antigens Proteins 0.000 description 3
• 102000036639 antigens Human genes 0.000 description 3
• 210000004369 blood Anatomy 0.000 description 3
• 239000008280 blood Substances 0.000 description 3
• 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
• 230000022159 cartilage development Effects 0.000 description 3
• 230000008859 change Effects 0.000 description 3
• 230000024203 complement activation Effects 0.000 description 3
• 230000000875 corresponding effect Effects 0.000 description 3
• 230000001351 cycling effect Effects 0.000 description 3
• 230000001419 dependent effect Effects 0.000 description 3
• 210000002555 descemet membrane Anatomy 0.000 description 3
• 229910000397 disodium phosphate Inorganic materials 0.000 description 3
• 231100000673 dose–response relationship Toxicity 0.000 description 3
• 238000007877 drug screening Methods 0.000 description 3
• 239000003623 enhancer Substances 0.000 description 3
• 210000002744 extracellular matrix Anatomy 0.000 description 3
• 239000000284 extract Substances 0.000 description 3
• 238000001943 fluorescence-activated cell sorting Methods 0.000 description 3
• 230000013595 glycosylation Effects 0.000 description 3
• 238000006206 glycosylation reaction Methods 0.000 description 3
• 230000012010 growth Effects 0.000 description 3
• 230000006266 hibernation Effects 0.000 description 3
• 210000000688 human artificial chromosome Anatomy 0.000 description 3
• 229910052739 hydrogen Inorganic materials 0.000 description 3
• 102000018358 immunoglobulin Human genes 0.000 description 3
• 238000001727 in vivo Methods 0.000 description 3
• 208000000509 infertility Diseases 0.000 description 3
• 230000036512 infertility Effects 0.000 description 3
• 208000027866 inflammatory disease Diseases 0.000 description 3
• 230000002401 inhibitory effect Effects 0.000 description 3
• 229940125396 insulin Drugs 0.000 description 3
• 210000003734 kidney Anatomy 0.000 description 3
• 229910052943 magnesium sulfate Inorganic materials 0.000 description 3
• 201000001441 melanoma Diseases 0.000 description 3
• 235000013336 milk Nutrition 0.000 description 3
• 239000008267 milk Substances 0.000 description 3
• 210000004080 milk Anatomy 0.000 description 3
• 238000010369 molecular cloning Methods 0.000 description 3
• 230000011164 ossification Effects 0.000 description 3
• 108010040377 otolin-1 Proteins 0.000 description 3
• 230000002842 otolith Effects 0.000 description 3
• 230000002018 overexpression Effects 0.000 description 3
• 229920001223 polyethylene glycol Polymers 0.000 description 3
• 102000040430 polynucleotide Human genes 0.000 description 3
• 108091033319 polynucleotide Proteins 0.000 description 3
• 239000002157 polynucleotide Substances 0.000 description 3
• 239000000843 powder Substances 0.000 description 3
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 3
• 239000011541 reaction mixture Substances 0.000 description 3
• 230000006798 recombination Effects 0.000 description 3
• 238000005215 recombination Methods 0.000 description 3
• 150000003839 salts Chemical class 0.000 description 3
• 238000007423 screening assay Methods 0.000 description 3
• 239000011780 sodium chloride Substances 0.000 description 3
• 229910000162 sodium phosphate Inorganic materials 0.000 description 3
• 238000010561 standard procedure Methods 0.000 description 3
• 229940124597 therapeutic agent Drugs 0.000 description 3
• 230000009466 transformation Effects 0.000 description 3
• 241001515965 unidentified phage Species 0.000 description 3
• 241001430294 unidentified retrovirus Species 0.000 description 3
• 238000005406 washing Methods 0.000 description 3
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
• 208000030507 AIDS Diseases 0.000 description 2
• 206010001513 AIDS related complex Diseases 0.000 description 2
• 206010001052 Acute respiratory distress syndrome Diseases 0.000 description 2
• 108010062271 Acute-Phase Proteins Proteins 0.000 description 2
• 102000011767 Acute-Phase Proteins Human genes 0.000 description 2
• 206010002556 Ankylosing Spondylitis Diseases 0.000 description 2
• 101100325793 Arabidopsis thaliana BCA2 gene Proteins 0.000 description 2
• 208000009137 Behcet syndrome Diseases 0.000 description 2
• 102000004506 Blood Proteins Human genes 0.000 description 2
• 108010017384 Blood Proteins Proteins 0.000 description 2
• 206010006895 Cachexia Diseases 0.000 description 2
• 208000024172 Cardiovascular disease Diseases 0.000 description 2
• 102000014914 Carrier Proteins Human genes 0.000 description 2
• 108010078791 Carrier Proteins Proteins 0.000 description 2
• 206010008874 Chronic Fatigue Syndrome Diseases 0.000 description 2
• 208000006545 Chronic Obstructive Pulmonary Disease Diseases 0.000 description 2
• 108091033380 Coding strand Proteins 0.000 description 2
• 108020004705 Codon Proteins 0.000 description 2
• 206010009900 Colitis ulcerative Diseases 0.000 description 2
• 102000001191 Collagen Type VIII Human genes 0.000 description 2
• 102100025890 Complement C1q tumor necrosis factor-related protein 3 Human genes 0.000 description 2
• 206010010741 Conjunctivitis Diseases 0.000 description 2
• 208000011231 Crohn disease Diseases 0.000 description 2
• 102000004127 Cytokines Human genes 0.000 description 2
• 108090000695 Cytokines Proteins 0.000 description 2
• 108010017826 DNA Polymerase I Proteins 0.000 description 2
• 102000004594 DNA Polymerase I Human genes 0.000 description 2
• 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
• 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 2
• 208000012239 Developmental disease Diseases 0.000 description 2
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
• 201000009273 Endometriosis Diseases 0.000 description 2
• 108010067770 Endopeptidase K Proteins 0.000 description 2
• 206010014824 Endotoxic shock Diseases 0.000 description 2
• 101710091045 Envelope protein Proteins 0.000 description 2
• 108050001049 Extracellular proteins Proteins 0.000 description 2
• ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
• 102000004961 Furin Human genes 0.000 description 2
• 102100035233 Furin Human genes 0.000 description 2
• 102100039556 Galectin-4 Human genes 0.000 description 2
• 201000005569 Gout Diseases 0.000 description 2
• 208000023661 Haematological disease Diseases 0.000 description 2
• 206010019280 Heart failures Diseases 0.000 description 2
• 208000017604 Hodgkin disease Diseases 0.000 description 2
• 208000010747 Hodgkins lymphoma Diseases 0.000 description 2
• 101000775469 Homo sapiens Adiponectin Proteins 0.000 description 2
• 101000608765 Homo sapiens Galectin-4 Proteins 0.000 description 2
• 101001114673 Homo sapiens Multimerin-1 Proteins 0.000 description 2
• 206010020460 Human T-cell lymphotropic virus type I infection Diseases 0.000 description 2
• 241000714260 Human T-lymphotropic virus 1 Species 0.000 description 2
• 206010020751 Hypersensitivity Diseases 0.000 description 2
• 208000022559 Inflammatory bowel disease Diseases 0.000 description 2
• 108091092195 Intron Proteins 0.000 description 2
• ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 2
• 208000019693 Lung disease Diseases 0.000 description 2
• 239000004472 Lysine Substances 0.000 description 2
• 241000829100 Macaca mulatta polyomavirus 1 Species 0.000 description 2
• CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
• 208000007466 Male Infertility Diseases 0.000 description 2
• 108010052285 Membrane Proteins Proteins 0.000 description 2
• 102000018697 Membrane Proteins Human genes 0.000 description 2
• 208000024556 Mendelian disease Diseases 0.000 description 2
• 101100095974 Mus musculus Smc3 gene Proteins 0.000 description 2
• 208000000112 Myalgia Diseases 0.000 description 2
• 208000014767 Myeloproliferative disease Diseases 0.000 description 2
• 201000007224 Myeloproliferative neoplasm Diseases 0.000 description 2
• 208000025966 Neurological disease Diseases 0.000 description 2
• 208000001132 Osteoporosis Diseases 0.000 description 2
• 206010033645 Pancreatitis Diseases 0.000 description 2
• 102000035195 Peptidases Human genes 0.000 description 2
• 239000002202 Polyethylene glycol Substances 0.000 description 2
• 102000017033 Porins Human genes 0.000 description 2
• 108010013381 Porins Proteins 0.000 description 2
• 101710188315 Protein X Proteins 0.000 description 2
• 201000004681 Psoriasis Diseases 0.000 description 2
• 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 2
• 108020004511 Recombinant DNA Proteins 0.000 description 2
• 206010063837 Reperfusion injury Diseases 0.000 description 2
• 208000013616 Respiratory Distress Syndrome Diseases 0.000 description 2
• 206010039491 Sarcoma Diseases 0.000 description 2
• 206010040047 Sepsis Diseases 0.000 description 2
• 108020004682 Single-Stranded DNA Proteins 0.000 description 2
• DMWVGXGXHPOEPT-UHFFFAOYSA-N Src Inhibitor-1 Chemical compound C=12C=C(OC)C(OC)=CC2=NC=NC=1NC(C=C1)=CC=C1OC1=CC=CC=C1 DMWVGXGXHPOEPT-UHFFFAOYSA-N 0.000 description 2
• 208000006011 Stroke Diseases 0.000 description 2
• QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 2
• 239000008049 TAE buffer Substances 0.000 description 2
• 108010006785 Taq Polymerase Proteins 0.000 description 2
• 102000002933 Thioredoxin Human genes 0.000 description 2
• 108010022394 Threonine synthase Proteins 0.000 description 2
• 241000723873 Tobacco mosaic virus Species 0.000 description 2
• 201000006704 Ulcerative Colitis Diseases 0.000 description 2
• DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 2
• 206010046851 Uveitis Diseases 0.000 description 2
• HGEVZDLYZYVYHD-UHFFFAOYSA-N acetic acid;2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid Chemical compound CC(O)=O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O HGEVZDLYZYVYHD-UHFFFAOYSA-N 0.000 description 2
• 239000002253 acid Substances 0.000 description 2
• 230000002730 additional effect Effects 0.000 description 2
• 210000001789 adipocyte Anatomy 0.000 description 2
• 230000032683 aging Effects 0.000 description 2
• 208000037883 airway inflammation Diseases 0.000 description 2
• 208000026935 allergic disease Diseases 0.000 description 2
• 230000007815 allergy Effects 0.000 description 2
• 210000004102 animal cell Anatomy 0.000 description 2
• 230000001028 anti-proliverative effect Effects 0.000 description 2
• 125000003118 aryl group Chemical group 0.000 description 2
• 208000006673 asthma Diseases 0.000 description 2
• 230000001363 autoimmune Effects 0.000 description 2
• 230000005784 autoimmunity Effects 0.000 description 2
• 210000002469 basement membrane Anatomy 0.000 description 2
• 230000033228 biological regulation Effects 0.000 description 2
• 230000000903 blocking effect Effects 0.000 description 2
• 239000011575 calcium Substances 0.000 description 2
• 229910052791 calcium Inorganic materials 0.000 description 2
• 230000015556 catabolic process Effects 0.000 description 2
• 239000007795 chemical reaction product Substances 0.000 description 2
• HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
• 238000011210 chromatographic step Methods 0.000 description 2
• 210000000349 chromosome Anatomy 0.000 description 2
• 239000013599 cloning vector Substances 0.000 description 2
• 210000001072 colon Anatomy 0.000 description 2
• 230000009918 complex formation Effects 0.000 description 2
• 210000002808 connective tissue Anatomy 0.000 description 2
• 238000010276 construction Methods 0.000 description 2
• 238000007796 conventional method Methods 0.000 description 2
• 210000000399 corneal endothelial cell Anatomy 0.000 description 2
• 238000004132 cross linking Methods 0.000 description 2
• 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 2
• 230000007123 defense Effects 0.000 description 2
• 238000006731 degradation reaction Methods 0.000 description 2
• 238000011161 development Methods 0.000 description 2
• 230000018109 developmental process Effects 0.000 description 2
• 238000002405 diagnostic procedure Methods 0.000 description 2
• 210000002249 digestive system Anatomy 0.000 description 2
• 102000004419 dihydrofolate reductase Human genes 0.000 description 2
• 229940079593 drug Drugs 0.000 description 2
• 238000007876 drug discovery Methods 0.000 description 2
• 210000002472 endoplasmic reticulum Anatomy 0.000 description 2
• 230000003511 endothelial effect Effects 0.000 description 2
• 210000000871 endothelium corneal Anatomy 0.000 description 2
• 230000037149 energy metabolism Effects 0.000 description 2
• 238000002474 experimental method Methods 0.000 description 2
• 210000003414 extremity Anatomy 0.000 description 2
• 210000002950 fibroblast Anatomy 0.000 description 2
• 238000002523 gelfiltration Methods 0.000 description 2
• 230000030279 gene silencing Effects 0.000 description 2
• 230000009368 gene silencing by RNA Effects 0.000 description 2
• 238000012226 gene silencing method Methods 0.000 description 2
• 208000016361 genetic disease Diseases 0.000 description 2
• 210000004349 growth plate Anatomy 0.000 description 2
• UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
• 210000003128 head Anatomy 0.000 description 2
• 239000005556 hormone Substances 0.000 description 2
• 229940088597 hormone Drugs 0.000 description 2
• 210000004408 hybridoma Anatomy 0.000 description 2
• 239000001257 hydrogen Substances 0.000 description 2
• 230000002209 hydrophobic effect Effects 0.000 description 2
• 230000033444 hydroxylation Effects 0.000 description 2
• 238000005805 hydroxylation reaction Methods 0.000 description 2
• 208000026278 immune system disease Diseases 0.000 description 2
• 230000006058 immune tolerance Effects 0.000 description 2
• 238000003018 immunoassay Methods 0.000 description 2
• 230000001976 improved effect Effects 0.000 description 2
• 238000007901 in situ hybridization Methods 0.000 description 2
• 230000002458 infectious effect Effects 0.000 description 2
• 231100000535 infertility Toxicity 0.000 description 2
• 230000000977 initiatory effect Effects 0.000 description 2
• 238000007918 intramuscular administration Methods 0.000 description 2
• 238000001990 intravenous administration Methods 0.000 description 2
• 150000002500 ions Chemical class 0.000 description 2
• 238000005304 joining Methods 0.000 description 2
• 238000007834 ligase chain reaction Methods 0.000 description 2
• 150000002632 lipids Chemical class 0.000 description 2
• 239000002502 liposome Substances 0.000 description 2
• 208000019423 liver disease Diseases 0.000 description 2
• 230000004807 localization Effects 0.000 description 2
• 230000007774 longterm Effects 0.000 description 2
• 210000002540 macrophage Anatomy 0.000 description 2
• 201000004792 malaria Diseases 0.000 description 2
• 238000013507 mapping Methods 0.000 description 2
• 208000030159 metabolic disease Diseases 0.000 description 2
• 201000006417 multiple sclerosis Diseases 0.000 description 2
• 210000003205 muscle Anatomy 0.000 description 2
• 208000029766 myalgic encephalomeyelitis/chronic fatigue syndrome Diseases 0.000 description 2
• 206010028417 myasthenia gravis Diseases 0.000 description 2
• 208000031225 myocardial ischemia Diseases 0.000 description 2
• 230000001338 necrotic effect Effects 0.000 description 2
• 230000001613 neoplastic effect Effects 0.000 description 2
• 230000003472 neutralizing effect Effects 0.000 description 2
• 238000007899 nucleic acid hybridization Methods 0.000 description 2
• 238000013116 obese mouse model Methods 0.000 description 2
• 201000008482 osteoarthritis Diseases 0.000 description 2
• 238000004806 packaging method and process Methods 0.000 description 2
• 230000036961 partial effect Effects 0.000 description 2
• 239000002245 particle Substances 0.000 description 2
• 150000003904 phospholipids Chemical class 0.000 description 2
• 102000054765 polymorphisms of proteins Human genes 0.000 description 2
• 230000001323 posttranslational effect Effects 0.000 description 2
• 238000002360 preparation method Methods 0.000 description 2
• 230000000861 pro-apoptotic effect Effects 0.000 description 2
• 230000002062 proliferating effect Effects 0.000 description 2
• 235000019419 proteases Nutrition 0.000 description 2
• 238000000159 protein binding assay Methods 0.000 description 2
• 230000002797 proteolythic effect Effects 0.000 description 2
• 230000006337 proteolytic cleavage Effects 0.000 description 2
• 230000009467 reduction Effects 0.000 description 2
• 230000010076 replication Effects 0.000 description 2
• 230000001850 reproductive effect Effects 0.000 description 2
• 230000000717 retained effect Effects 0.000 description 2
• 230000001177 retroviral effect Effects 0.000 description 2
• 206010039073 rheumatoid arthritis Diseases 0.000 description 2
• 206010039083 rhinitis Diseases 0.000 description 2
• 230000035945 sensitivity Effects 0.000 description 2
• 230000036303 septic shock Effects 0.000 description 2
• 210000002966 serum Anatomy 0.000 description 2
• 230000011664 signaling Effects 0.000 description 2
• 241000894007 species Species 0.000 description 2
• 230000009870 specific binding Effects 0.000 description 2
• 238000007920 subcutaneous administration Methods 0.000 description 2
• 208000009996 subepithelial mucinous corneal dystrophy Diseases 0.000 description 2
• 229910021653 sulphate ion Inorganic materials 0.000 description 2
• 239000006228 supernatant Substances 0.000 description 2
• 238000003786 synthesis reaction Methods 0.000 description 2
• 201000000596 systemic lupus erythematosus Diseases 0.000 description 2
• 230000002123 temporal effect Effects 0.000 description 2
• 108060008226 thioredoxin Proteins 0.000 description 2
• 229940094937 thioredoxin Drugs 0.000 description 2
• RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
• 238000012546 transfer Methods 0.000 description 2
• 230000005945 translocation Effects 0.000 description 2
• 230000009452 underexpressoin Effects 0.000 description 2
• 241000701161 unidentified adenovirus Species 0.000 description 2
• 239000003981 vehicle Substances 0.000 description 2
• 230000004393 visual impairment Effects 0.000 description 2
• 108010047303 von Willebrand Factor Proteins 0.000 description 2
• 102100036537 von Willebrand factor Human genes 0.000 description 2
• 229960001134 von willebrand factor Drugs 0.000 description 2
• 230000029663 wound healing Effects 0.000 description 2
• ADHFFUOAOLWHGU-JPDUFPOXSA-N (2s)-2-[[(2s)-4-amino-2-[[(2s,3r)-2-[[(2s)-2-[[(2s)-2-[[(2s,3s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-6-amino-2-[[(2s)-2-[[(2s)-2-[[2-[[(2s)-2-amino-3-hydroxypropanoyl]amino]acetyl]amino]-3-hydroxypropanoyl]amino]propanoyl]amino]hexanoyl]a Chemical compound C([C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=1NC=NC=1)C(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@@H](N)CO)C(C)C)C1=CC=CC=C1 ADHFFUOAOLWHGU-JPDUFPOXSA-N 0.000 description 1
• MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 1
• UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 1
• JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
• QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
• QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 1
• TXUWMXQFNYDOEZ-UHFFFAOYSA-N 5-(1H-indol-3-ylmethyl)-3-methyl-2-sulfanylidene-4-imidazolidinone Chemical compound O=C1N(C)C(=S)NC1CC1=CNC2=CC=CC=C12 TXUWMXQFNYDOEZ-UHFFFAOYSA-N 0.000 description 1
• ODHCTXKNWHHXJC-VKHMYHEASA-N 5-oxo-L-proline Chemical compound OC(=O)[C@@H]1CCC(=O)N1 ODHCTXKNWHHXJC-VKHMYHEASA-N 0.000 description 1
• 101150112998 ADIPOQ gene Proteins 0.000 description 1
• 230000005730 ADP ribosylation Effects 0.000 description 1
• 101150096655 APM1 gene Proteins 0.000 description 1
• 101150094949 APRT gene Proteins 0.000 description 1
• 102000007481 Activating Transcription Factor 6 Human genes 0.000 description 1
• 108010085405 Activating Transcription Factor 6 Proteins 0.000 description 1
• 229930024421 Adenine Natural products 0.000 description 1
• GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
• 102100029457 Adenine phosphoribosyltransferase Human genes 0.000 description 1
• 108010024223 Adenine phosphoribosyltransferase Proteins 0.000 description 1
• 108700028369 Alleles Proteins 0.000 description 1
• 244000153158 Ammi visnaga Species 0.000 description 1
• 235000010585 Ammi visnaga Nutrition 0.000 description 1
• 208000037259 Amyloid Plaque Diseases 0.000 description 1
• 102000002659 Amyloid Precursor Protein Secretases Human genes 0.000 description 1
• 108010043324 Amyloid Precursor Protein Secretases Proteins 0.000 description 1
• 108020004491 Antisense DNA Proteins 0.000 description 1
• 102000013918 Apolipoproteins E Human genes 0.000 description 1
• 108010025628 Apolipoproteins E Proteins 0.000 description 1
• 101100519158 Arabidopsis thaliana PCR2 gene Proteins 0.000 description 1
• 241000712892 Arenaviridae Species 0.000 description 1
• 208000037260 Atherosclerotic Plaque Diseases 0.000 description 1
• 241000972773 Aulopiformes Species 0.000 description 1
• 244000063299 Bacillus subtilis Species 0.000 description 1
• 235000014469 Bacillus subtilis Nutrition 0.000 description 1
• 108010077805 Bacterial Proteins Proteins 0.000 description 1
• 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
• DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
• 101800000285 Big gastrin Proteins 0.000 description 1
• 101000800130 Bos taurus Thyroglobulin Proteins 0.000 description 1
• 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
• 102000004219 Brain-derived neurotrophic factor Human genes 0.000 description 1
• 108090000715 Brain-derived neurotrophic factor Proteins 0.000 description 1
• 206010006187 Breast cancer Diseases 0.000 description 1
• 102100021935 C-C motif chemokine 26 Human genes 0.000 description 1
• OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
• 101001007681 Candida albicans (strain WO-1) Kexin Proteins 0.000 description 1
• 241000282472 Canis lupus familiaris Species 0.000 description 1
• 241000283707 Capra Species 0.000 description 1
• 108090000565 Capsid Proteins Proteins 0.000 description 1
• 241000701489 Cauliflower mosaic virus Species 0.000 description 1
• 241000282693 Cercopithecidae Species 0.000 description 1
• 101800001299 Cerebellin Proteins 0.000 description 1
• 102400001244 Cerebellin Human genes 0.000 description 1
• 102100035244 Cerebellin-1 Human genes 0.000 description 1
• 101710135505 Cerebellin-1 Proteins 0.000 description 1
• 102100023321 Ceruloplasmin Human genes 0.000 description 1
• 108010012236 Chemokines Proteins 0.000 description 1
• 102000019034 Chemokines Human genes 0.000 description 1
• 239000005496 Chlorsulfuron Substances 0.000 description 1
• 206010008631 Cholera Diseases 0.000 description 1
• 108010077544 Chromatin Proteins 0.000 description 1
• 108700010070 Codon Usage Proteins 0.000 description 1
• 101710204075 Complement C1q tumor necrosis factor-related protein 3 Proteins 0.000 description 1
• 238000011537 Coomassie blue staining Methods 0.000 description 1
• 206010052117 Corneal decompensation Diseases 0.000 description 1
• 229920000742 Cotton Polymers 0.000 description 1
• 241000699800 Cricetinae Species 0.000 description 1
• 241000699802 Cricetulus griseus Species 0.000 description 1
• UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 1
• 102000003915 DNA Topoisomerases Human genes 0.000 description 1
• 108090000323 DNA Topoisomerases Proteins 0.000 description 1
• 239000003298 DNA probe Substances 0.000 description 1
• 230000004568 DNA-binding Effects 0.000 description 1
• 102100035784 Decorin Human genes 0.000 description 1
• 108090000738 Decorin Proteins 0.000 description 1
• 241000702421 Dependoparvovirus Species 0.000 description 1
• 101100162826 Dictyostelium discoideum apm2 gene Proteins 0.000 description 1
• BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 1
• 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
• 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
• 238000012286 ELISA Assay Methods 0.000 description 1
• 102000004533 Endonucleases Human genes 0.000 description 1
• 108010042407 Endonucleases Proteins 0.000 description 1
• 102000005593 Endopeptidases Human genes 0.000 description 1
• 108010059378 Endopeptidases Proteins 0.000 description 1
• 241000991587 Enterovirus C Species 0.000 description 1
• 101710121417 Envelope glycoprotein Proteins 0.000 description 1
• 108010008655 Epstein-Barr Virus Nuclear Antigens Proteins 0.000 description 1
• 241000283073 Equus caballus Species 0.000 description 1
• 108060002716 Exonuclease Proteins 0.000 description 1
• 108010074105 Factor Va Proteins 0.000 description 1
• 108010074860 Factor Xa Proteins 0.000 description 1
• 241000700662 Fowlpox virus Species 0.000 description 1
• 206010017577 Gait disturbance Diseases 0.000 description 1
• CHDWDBPJOZVZSE-KKUMJFAQSA-N Glu-Phe-Met Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCSC)C(O)=O CHDWDBPJOZVZSE-KKUMJFAQSA-N 0.000 description 1
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Natural products NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 1
• 239000004471 Glycine Substances 0.000 description 1
• 102000003886 Glycoproteins Human genes 0.000 description 1
• 108090000288 Glycoproteins Proteins 0.000 description 1
• 241000219146 Gossypium Species 0.000 description 1
• 239000007995 HEPES buffer Substances 0.000 description 1
• 101710154606 Hemagglutinin Proteins 0.000 description 1
• 208000028782 Hereditary disease Diseases 0.000 description 1
• 108010093488 His-His-His-His-His-His Proteins 0.000 description 1
• 108091006054 His-tagged proteins Proteins 0.000 description 1
• 101000897493 Homo sapiens C-C motif chemokine 26 Proteins 0.000 description 1
• 101000737277 Homo sapiens Cerebellin-1 Proteins 0.000 description 1
• 101000875027 Homo sapiens Collagen alpha-1(X) chain Proteins 0.000 description 1
• 101000933673 Homo sapiens Complement C1q tumor necrosis factor-related protein 3 Proteins 0.000 description 1
• 101001128694 Homo sapiens Neuroendocrine convertase 1 Proteins 0.000 description 1
• 101000601394 Homo sapiens Neuroendocrine convertase 2 Proteins 0.000 description 1
• 101001082142 Homo sapiens Pentraxin-related protein PTX3 Proteins 0.000 description 1
• 101100053794 Homo sapiens ZBTB7C gene Proteins 0.000 description 1
• CPELXLSAUQHCOX-UHFFFAOYSA-N Hydrogen bromide Chemical class Br CPELXLSAUQHCOX-UHFFFAOYSA-N 0.000 description 1
• PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 1
• 206010060378 Hyperinsulinaemia Diseases 0.000 description 1
• 108700009884 Hypoadiponectinemia Proteins 0.000 description 1
• 102000009786 Immunoglobulin Constant Regions Human genes 0.000 description 1
• 108010009817 Immunoglobulin Constant Regions Proteins 0.000 description 1
• 101710096444 Killer toxin Proteins 0.000 description 1
• 241000588748 Klebsiella Species 0.000 description 1
• ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
• 229930182816 L-glutamine Natural products 0.000 description 1
• FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
• FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
• 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
• 108090001090 Lectins Proteins 0.000 description 1
• 102000004856 Lectins Human genes 0.000 description 1
• 241000721654 Lepomis macrochirus Species 0.000 description 1
• 239000005089 Luciferase Substances 0.000 description 1
• 241000124008 Mammalia Species 0.000 description 1
• 102000011716 Matrix Metalloproteinase 14 Human genes 0.000 description 1
• 108010076557 Matrix Metalloproteinase 14 Proteins 0.000 description 1
• 108010090054 Membrane Glycoproteins Proteins 0.000 description 1
• 102000012750 Membrane Glycoproteins Human genes 0.000 description 1
• 101000775476 Mus musculus Adiponectin Proteins 0.000 description 1
• 241000699670 Mus sp. Species 0.000 description 1
• 108010057466 NF-kappa B Proteins 0.000 description 1
• 102000003945 NF-kappa B Human genes 0.000 description 1
• 108091061960 Naked DNA Proteins 0.000 description 1
• 229920002274 Nalgene Polymers 0.000 description 1
• 206010028851 Necrosis Diseases 0.000 description 1
• 229930193140 Neomycin Natural products 0.000 description 1
• 108010025020 Nerve Growth Factor Proteins 0.000 description 1
• 102000015336 Nerve Growth Factor Human genes 0.000 description 1
• 102100032132 Neuroendocrine convertase 1 Human genes 0.000 description 1
• 108090000189 Neuropeptides Proteins 0.000 description 1
• 102000003797 Neuropeptides Human genes 0.000 description 1
• 239000000020 Nitrocellulose Substances 0.000 description 1
• 238000000636 Northern blotting Methods 0.000 description 1
• 101710163270 Nuclease Proteins 0.000 description 1
• 108091005461 Nucleic proteins Proteins 0.000 description 1
• 108700020497 Nucleopolyhedrovirus polyhedrin Proteins 0.000 description 1
• 239000004677 Nylon Substances 0.000 description 1
• 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
• 102000015636 Oligopeptides Human genes 0.000 description 1
• 108010038807 Oligopeptides Proteins 0.000 description 1
• 101100242355 Oncorhynchus keta otol1 gene Proteins 0.000 description 1
• 108700022034 Opsonin Proteins Proteins 0.000 description 1
• 101710093908 Outer capsid protein VP4 Proteins 0.000 description 1
• 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 1
• 229910019142 PO4 Inorganic materials 0.000 description 1
• 208000037273 Pathologic Processes Diseases 0.000 description 1
• BFHAYPLBUQVNNJ-UHFFFAOYSA-N Pectenotoxin 3 Natural products OC1C(C)CCOC1(O)C1OC2C=CC(C)=CC(C)CC(C)(O3)CCC3C(O3)(O4)CCC3(C=O)CC4C(O3)C(=O)CC3(C)C(O)C(O3)CCC3(O3)CCCC3C(C)C(=O)OC2C1 BFHAYPLBUQVNNJ-UHFFFAOYSA-N 0.000 description 1
• 102100027351 Pentraxin-related protein PTX3 Human genes 0.000 description 1
• 108010067902 Peptide Library Proteins 0.000 description 1
• 101000973669 Petunia hybrida Bidirectional sugar transporter NEC1 Proteins 0.000 description 1
• 206010057249 Phagocytosis Diseases 0.000 description 1
• 102000004861 Phosphoric Diester Hydrolases Human genes 0.000 description 1
• 108090001050 Phosphoric Diester Hydrolases Proteins 0.000 description 1
• 241000709664 Picornaviridae Species 0.000 description 1
• 239000004698 Polyethylene Substances 0.000 description 1
• 229920000954 Polyglycolide Polymers 0.000 description 1
• 229920001213 Polysorbate 20 Polymers 0.000 description 1
• 108010069820 Pro-Opiomelanocortin Proteins 0.000 description 1
• 239000000683 Pro-Opiomelanocortin Substances 0.000 description 1
• 102100027467 Pro-opiomelanocortin Human genes 0.000 description 1
• 102100038931 Proenkephalin-A Human genes 0.000 description 1
• 102100024622 Proenkephalin-B Human genes 0.000 description 1
• ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
• 102000004079 Prolyl Hydroxylases Human genes 0.000 description 1
• 108010043005 Prolyl Hydroxylases Proteins 0.000 description 1
• XBDQKXXYIPTUBI-UHFFFAOYSA-N Propionic acid Chemical class CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 1
• 108090000545 Proprotein Convertase 2 Proteins 0.000 description 1
• 101710180552 Proprotein convertase subtilisin/kexin type 6 Proteins 0.000 description 1
• 101710176177 Protein A56 Proteins 0.000 description 1
• 108020004518 RNA Probes Proteins 0.000 description 1
• 239000003391 RNA probe Substances 0.000 description 1
• 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
• 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
• 108700005075 Regulator Genes Proteins 0.000 description 1
• 108090000783 Renin Proteins 0.000 description 1
• 108700008625 Reporter Genes Proteins 0.000 description 1
• AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
• 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 1
• 241000283984 Rodentia Species 0.000 description 1
• 240000000111 Saccharum officinarum Species 0.000 description 1
• 235000007201 Saccharum officinarum Nutrition 0.000 description 1
• 241000555745 Sciuridae Species 0.000 description 1
• 208000034189 Sclerosis Diseases 0.000 description 1
• 108091081021 Sense strand Proteins 0.000 description 1
• 208000020221 Short stature Diseases 0.000 description 1
• 241000700584 Simplexvirus Species 0.000 description 1
• 206010072610 Skeletal dysplasia Diseases 0.000 description 1
• 241000256248 Spodoptera Species 0.000 description 1
• 241000295644 Staphylococcaceae Species 0.000 description 1
• 102000009822 Sterol Regulatory Element Binding Proteins Human genes 0.000 description 1
• 108010020396 Sterol Regulatory Element Binding Proteins Proteins 0.000 description 1
• 101710135785 Subtilisin-like protease Proteins 0.000 description 1
• 235000021536 Sugar beet Nutrition 0.000 description 1
• 241000701093 Suid alphaherpesvirus 1 Species 0.000 description 1
• 241000282887 Suidae Species 0.000 description 1
• 206010043376 Tetanus Diseases 0.000 description 1
• 108091036066 Three prime untranslated region Proteins 0.000 description 1
• 102000006601 Thymidine Kinase Human genes 0.000 description 1
• 108020004440 Thymidine kinase Proteins 0.000 description 1
• 102000009843 Thyroglobulin Human genes 0.000 description 1
• 108091023040 Transcription factor Proteins 0.000 description 1
• 102000040945 Transcription factor Human genes 0.000 description 1
• 108020004566 Transfer RNA Proteins 0.000 description 1
• 102000046299 Transforming Growth Factor beta1 Human genes 0.000 description 1
• 101800002279 Transforming growth factor beta-1 Proteins 0.000 description 1
• 102000019250 Transforming growth factor-beta-related Human genes 0.000 description 1
• 108050006581 Transforming growth factor-beta-related Proteins 0.000 description 1
• 101800000385 Transmembrane protein Proteins 0.000 description 1
• 101800001690 Transmembrane protein gp41 Proteins 0.000 description 1
• 239000007984 Tris EDTA buffer Substances 0.000 description 1
• 241000700618 Vaccinia virus Species 0.000 description 1
• 108010067390 Viral Proteins Proteins 0.000 description 1
• 230000010530 Virus Neutralization Effects 0.000 description 1
• 206010047571 Visual impairment Diseases 0.000 description 1
• IXKSXJFAGXLQOQ-XISFHERQSA-N WHWLQLKPGQPMY Chemical compound C([C@@H](C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)NC(=O)[C@@H](N)CC=1C2=CC=CC=C2NC=1)C1=CNC=N1 IXKSXJFAGXLQOQ-XISFHERQSA-N 0.000 description 1
• 208000000260 Warts Diseases 0.000 description 1
• 230000001594 aberrant effect Effects 0.000 description 1
• 238000002679 ablation Methods 0.000 description 1
• 230000005856 abnormality Effects 0.000 description 1
• 150000001242 acetic acid derivatives Chemical class 0.000 description 1
• 230000021736 acetylation Effects 0.000 description 1
• 238000006640 acetylation reaction Methods 0.000 description 1
• 102000005421 acetyltransferase Human genes 0.000 description 1
• 108020002494 acetyltransferase Proteins 0.000 description 1
• 230000002378 acidificating effect Effects 0.000 description 1
• 230000009471 action Effects 0.000 description 1
• 239000012190 activator Substances 0.000 description 1
• 230000010933 acylation Effects 0.000 description 1
• 238000005917 acylation reaction Methods 0.000 description 1
• 229960000643 adenine Drugs 0.000 description 1
• 210000000577 adipose tissue Anatomy 0.000 description 1
• 239000002671 adjuvant Substances 0.000 description 1
• 238000001261 affinity purification Methods 0.000 description 1
• 238000013019 agitation Methods 0.000 description 1
• PPQRONHOSHZGFQ-LMVFSUKVSA-N aldehydo-D-ribose 5-phosphate Chemical group OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PPQRONHOSHZGFQ-LMVFSUKVSA-N 0.000 description 1
• 230000009435 amidation Effects 0.000 description 1
• 238000007112 amidation reaction Methods 0.000 description 1
• 229940126575 aminoglycoside Drugs 0.000 description 1
• BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
• 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
• 239000001166 ammonium sulphate Substances 0.000 description 1
• 235000011130 ammonium sulphate Nutrition 0.000 description 1
• 238000004873 anchoring Methods 0.000 description 1
• 208000036878 aneuploidy Diseases 0.000 description 1
• 231100001075 aneuploidy Toxicity 0.000 description 1
• 125000000129 anionic group Chemical group 0.000 description 1
• 150000001450 anions Chemical class 0.000 description 1
• 230000000340 anti-metabolite Effects 0.000 description 1
• 229940100197 antimetabolite Drugs 0.000 description 1
• 239000002256 antimetabolite Substances 0.000 description 1
• 239000002246 antineoplastic agent Substances 0.000 description 1
• 229940041181 antineoplastic drug Drugs 0.000 description 1
• 239000003963 antioxidant agent Substances 0.000 description 1
• 235000006708 antioxidants Nutrition 0.000 description 1
• 239000003816 antisense DNA Substances 0.000 description 1
• 230000010516 arginylation Effects 0.000 description 1
• 210000001106 artificial yeast chromosome Anatomy 0.000 description 1
• 238000011888 autopsy Methods 0.000 description 1
• 208000021018 autosomal dominant inheritance Diseases 0.000 description 1
• 210000003719 b-lymphocyte Anatomy 0.000 description 1
• 108010058966 bacteriophage T7 induced DNA polymerase Proteins 0.000 description 1
• 239000011324 bead Substances 0.000 description 1
• 150000001558 benzoic acid derivatives Chemical class 0.000 description 1
• DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 1
• 238000004166 bioassay Methods 0.000 description 1
• 230000003115 biocidal effect Effects 0.000 description 1
• 230000008827 biological function Effects 0.000 description 1
• 230000031018 biological processes and functions Effects 0.000 description 1
• 230000033558 biomineral tissue development Effects 0.000 description 1
• 238000001574 biopsy Methods 0.000 description 1
• 208000002352 blister Diseases 0.000 description 1
• 229940098773 bovine serum albumin Drugs 0.000 description 1
• 229940077737 brain-derived neurotrophic factor Drugs 0.000 description 1
• 230000002308 calcification Effects 0.000 description 1
• 150000001669 calcium Chemical class 0.000 description 1
• 239000001506 calcium phosphate Substances 0.000 description 1
• 229910000389 calcium phosphate Inorganic materials 0.000 description 1
• 235000011010 calcium phosphates Nutrition 0.000 description 1
• 239000002775 capsule Substances 0.000 description 1
• 239000003054 catalyst Substances 0.000 description 1
• 230000021164 cell adhesion Effects 0.000 description 1
• 230000024245 cell differentiation Effects 0.000 description 1
• 238000005119 centrifugation Methods 0.000 description 1
• 238000012512 characterization method Methods 0.000 description 1
• 238000012412 chemical coupling Methods 0.000 description 1
• 238000001311 chemical methods and process Methods 0.000 description 1
• 238000007385 chemical modification Methods 0.000 description 1
• 108700010039 chimeric receptor Proteins 0.000 description 1
• VJYIFXVZLXQVHO-UHFFFAOYSA-N chlorsulfuron Chemical compound COC1=NC(C)=NC(NC(=O)NS(=O)(=O)C=2C(=CC=CC=2)Cl)=N1 VJYIFXVZLXQVHO-UHFFFAOYSA-N 0.000 description 1
• 235000012000 cholesterol Nutrition 0.000 description 1
• 230000031154 cholesterol homeostasis Effects 0.000 description 1
• 210000003483 chromatin Anatomy 0.000 description 1
• 230000014107 chromosome localization Effects 0.000 description 1
• 230000000052 comparative effect Effects 0.000 description 1
• 238000012875 competitive assay Methods 0.000 description 1
• 230000002860 competitive effect Effects 0.000 description 1
• 102000006834 complement receptors Human genes 0.000 description 1
• 108010047295 complement receptors Proteins 0.000 description 1
• 230000004154 complement system Effects 0.000 description 1
• 238000012790 confirmation Methods 0.000 description 1
• 230000021615 conjugation Effects 0.000 description 1
• 238000011109 contamination Methods 0.000 description 1
• 229920001577 copolymer Polymers 0.000 description 1
• 210000004087 cornea Anatomy 0.000 description 1
• 230000002596 correlated effect Effects 0.000 description 1
• 238000012866 crystallographic experiment Methods 0.000 description 1
• 210000004748 cultured cell Anatomy 0.000 description 1
• 235000018417 cysteine Nutrition 0.000 description 1
• XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
• UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 1
• 230000009089 cytolysis Effects 0.000 description 1
• 210000000805 cytoplasm Anatomy 0.000 description 1
• 230000007812 deficiency Effects 0.000 description 1
• 230000017858 demethylation Effects 0.000 description 1
• 238000010520 demethylation reaction Methods 0.000 description 1
• 230000008021 deposition Effects 0.000 description 1
• 238000013461 design Methods 0.000 description 1
• 235000005911 diet Nutrition 0.000 description 1
• 230000037213 diet Effects 0.000 description 1
• 230000004069 differentiation Effects 0.000 description 1
• 230000029087 digestion Effects 0.000 description 1
• 239000000539 dimer Substances 0.000 description 1
• 206010013023 diphtheria Diseases 0.000 description 1
• BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
• 235000019800 disodium phosphate Nutrition 0.000 description 1
• 238000006073 displacement reaction Methods 0.000 description 1
• PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
• 239000008298 dragée Substances 0.000 description 1
• 239000000890 drug combination Substances 0.000 description 1
• 238000007878 drug screening assay Methods 0.000 description 1
• 230000004064 dysfunction Effects 0.000 description 1
• 235000013399 edible fruits Nutrition 0.000 description 1
• 238000001962 electrophoresis Methods 0.000 description 1
• 238000004520 electroporation Methods 0.000 description 1
• 238000010828 elution Methods 0.000 description 1
• 239000003995 emulsifying agent Substances 0.000 description 1
• 210000003890 endocrine cell Anatomy 0.000 description 1
• 210000000981 epithelium Anatomy 0.000 description 1
• 238000012869 ethanol precipitation Methods 0.000 description 1
• 102000013165 exonuclease Human genes 0.000 description 1
• 239000013613 expression plasmid Substances 0.000 description 1
• 238000000605 extraction Methods 0.000 description 1
• 230000004319 fatty acid homeostasis Effects 0.000 description 1
• 230000035558 fertility Effects 0.000 description 1
• 238000002866 fluorescence resonance energy transfer Methods 0.000 description 1
• 239000007850 fluorescent dye Substances 0.000 description 1
• 230000008217 follicular development Effects 0.000 description 1
• 230000037406 food intake Effects 0.000 description 1
• 230000022244 formylation Effects 0.000 description 1
• 238000006170 formylation reaction Methods 0.000 description 1
• 238000006062 fragmentation reaction Methods 0.000 description 1
• 125000000524 functional group Chemical group 0.000 description 1
• 230000002538 fungal effect Effects 0.000 description 1
• 238000007499 fusion processing Methods 0.000 description 1
• 230000006251 gamma-carboxylation Effects 0.000 description 1
• 238000001502 gel electrophoresis Methods 0.000 description 1
• 238000001476 gene delivery Methods 0.000 description 1
• 238000007429 general method Methods 0.000 description 1
• 210000004602 germ cell Anatomy 0.000 description 1
• 239000011521 glass Substances 0.000 description 1
• 239000008103 glucose Substances 0.000 description 1
• 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 1
• 150000004676 glycans Chemical class 0.000 description 1
• 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 1
• 239000003102 growth factor Substances 0.000 description 1
• 239000001963 growth medium Substances 0.000 description 1
• 238000003306 harvesting Methods 0.000 description 1
• 230000036541 health Effects 0.000 description 1
• 230000011132 hemopoiesis Effects 0.000 description 1
• 206010073071 hepatocellular carcinoma Diseases 0.000 description 1
• 231100000844 hepatocellular carcinoma Toxicity 0.000 description 1
• 230000002363 herbicidal effect Effects 0.000 description 1
• 239000004009 herbicide Substances 0.000 description 1
• 238000004128 high performance liquid chromatography Methods 0.000 description 1
• 238000013537 high throughput screening Methods 0.000 description 1
• 150000002411 histidines Chemical class 0.000 description 1
• 210000003917 human chromosome Anatomy 0.000 description 1
• 210000003035 hyaline cartilage Anatomy 0.000 description 1
• 150000003840 hydrochlorides Chemical class 0.000 description 1
• 238000004191 hydrophobic interaction chromatography Methods 0.000 description 1
• 238000012872 hydroxylapatite chromatography Methods 0.000 description 1
• 229960002591 hydroxyproline Drugs 0.000 description 1
• 201000001421 hyperglycemia Diseases 0.000 description 1
• 230000003451 hyperinsulinaemic effect Effects 0.000 description 1
• 201000008980 hyperinsulinism Diseases 0.000 description 1
• 230000036737 immune function Effects 0.000 description 1
• 210000000987 immune system Anatomy 0.000 description 1
• 230000036039 immunity Effects 0.000 description 1
• 230000000984 immunochemical effect Effects 0.000 description 1
• 230000005847 immunogenicity Effects 0.000 description 1
• 239000003022 immunostimulating agent Substances 0.000 description 1
• 230000001771 impaired effect Effects 0.000 description 1
• 239000012535 impurity Substances 0.000 description 1
• 230000006698 induction Effects 0.000 description 1
• 208000021267 infertility disease Diseases 0.000 description 1
• 230000028709 inflammatory response Effects 0.000 description 1
• 206010022000 influenza Diseases 0.000 description 1
• 230000015788 innate immune response Effects 0.000 description 1
• 239000002054 inoculum Substances 0.000 description 1
• 229910052500 inorganic mineral Inorganic materials 0.000 description 1
• 230000010354 integration Effects 0.000 description 1
• 238000001361 intraarterial administration Methods 0.000 description 1
• 230000003834 intracellular effect Effects 0.000 description 1
• 210000004020 intracellular membrane Anatomy 0.000 description 1
• 238000007912 intraperitoneal administration Methods 0.000 description 1
• 238000007913 intrathecal administration Methods 0.000 description 1
• 238000007914 intraventricular administration Methods 0.000 description 1
• 238000007852 inverse PCR Methods 0.000 description 1
• 238000011835 investigation Methods 0.000 description 1
• 230000026045 iodination Effects 0.000 description 1
• 238000006192 iodination reaction Methods 0.000 description 1
• 230000001788 irregular Effects 0.000 description 1
• 229960000318 kanamycin Drugs 0.000 description 1
• 229930027917 kanamycin Natural products 0.000 description 1
• SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
• 229930182823 kanamycin A Natural products 0.000 description 1
• 108010045069 keyhole-limpet hemocyanin Proteins 0.000 description 1
• 210000003127 knee Anatomy 0.000 description 1
• 238000011813 knockout mouse model Methods 0.000 description 1
• 238000002372 labelling Methods 0.000 description 1
• 101150066555 lacZ gene Proteins 0.000 description 1
• 230000002045 lasting effect Effects 0.000 description 1
• 239000002523 lectin Substances 0.000 description 1
• 235000021374 legumes Nutrition 0.000 description 1
• 230000003902 lesion Effects 0.000 description 1
• GZQKNULLWNGMCW-PWQABINMSA-N lipid A (E. coli) Chemical compound O1[C@H](CO)[C@@H](OP(O)(O)=O)[C@H](OC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCCCC)[C@@H](NC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCC)[C@@H]1OC[C@@H]1[C@@H](O)[C@H](OC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](NC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](OP(O)(O)=O)O1 GZQKNULLWNGMCW-PWQABINMSA-N 0.000 description 1
• 239000007791 liquid phase Substances 0.000 description 1
• 239000006193 liquid solution Substances 0.000 description 1
• 239000006194 liquid suspension Substances 0.000 description 1
• 210000004185 liver Anatomy 0.000 description 1
• 238000011068 loading method Methods 0.000 description 1
• 238000004020 luminiscence type Methods 0.000 description 1
• 210000004072 lung Anatomy 0.000 description 1
• 208000020816 lung neoplasm Diseases 0.000 description 1
• 208000037841 lung tumor Diseases 0.000 description 1
• 210000004698 lymphocyte Anatomy 0.000 description 1
• 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
• 229920002521 macromolecule Polymers 0.000 description 1
• 235000019341 magnesium sulphate Nutrition 0.000 description 1
• 239000006249 magnetic particle Substances 0.000 description 1
• 150000002690 malonic acid derivatives Chemical class 0.000 description 1
• 238000005259 measurement Methods 0.000 description 1
• 230000008018 melting Effects 0.000 description 1
• 238000002844 melting Methods 0.000 description 1
• 230000002503 metabolic effect Effects 0.000 description 1
• 230000037353 metabolic pathway Effects 0.000 description 1
• 230000004060 metabolic process Effects 0.000 description 1
• 229910021645 metal ion Inorganic materials 0.000 description 1
• 150000002739 metals Chemical class 0.000 description 1
• MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
• 229930182817 methionine Natural products 0.000 description 1
• 229960000485 methotrexate Drugs 0.000 description 1
• 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
• 230000011987 methylation Effects 0.000 description 1
• 238000007069 methylation reaction Methods 0.000 description 1
• 244000005700 microbiome Species 0.000 description 1
• 238000000520 microinjection Methods 0.000 description 1
• 239000011707 mineral Substances 0.000 description 1
• 235000010755 mineral Nutrition 0.000 description 1
• 230000036456 mitotic arrest Effects 0.000 description 1
• ZAHQPTJLOCWVPG-UHFFFAOYSA-N mitoxantrone dihydrochloride Chemical compound Cl.Cl.O=C1C2=C(O)C=CC(O)=C2C(=O)C2=C1C(NCCNCCO)=CC=C2NCCNCCO ZAHQPTJLOCWVPG-UHFFFAOYSA-N 0.000 description 1
• 230000037230 mobility Effects 0.000 description 1
• 238000010172 mouse model Methods 0.000 description 1
• 108010012891 multimerin Proteins 0.000 description 1
• 238000002703 mutagenesis Methods 0.000 description 1
• 231100000350 mutagenesis Toxicity 0.000 description 1
• 230000007498 myristoylation Effects 0.000 description 1
• 210000004897 n-terminal region Anatomy 0.000 description 1
• 229930014626 natural product Natural products 0.000 description 1
• 230000017074 necrotic cell death Effects 0.000 description 1
• 230000010807 negative regulation of binding Effects 0.000 description 1
• 229960004927 neomycin Drugs 0.000 description 1
• 210000005170 neoplastic cell Anatomy 0.000 description 1
• 229940053128 nerve growth factor Drugs 0.000 description 1
• 210000003061 neural cell Anatomy 0.000 description 1
• 230000001537 neural effect Effects 0.000 description 1
• 238000006386 neutralization reaction Methods 0.000 description 1
• 229910000363 nickel(II) sulfate Inorganic materials 0.000 description 1
• 229920001220 nitrocellulos Polymers 0.000 description 1
• 230000006911 nucleation Effects 0.000 description 1
• 238000010899 nucleation Methods 0.000 description 1
• 230000031787 nutrient reservoir activity Effects 0.000 description 1
• 229920001778 nylon Polymers 0.000 description 1
• 239000002751 oligonucleotide probe Substances 0.000 description 1
• 238000002515 oligonucleotide synthesis Methods 0.000 description 1
• 238000011275 oncology therapy Methods 0.000 description 1
• 230000005305 organ development Effects 0.000 description 1
• 150000007524 organic acids Chemical class 0.000 description 1
• 235000005985 organic acids Nutrition 0.000 description 1
• 230000008520 organization Effects 0.000 description 1
• 210000001672 ovary Anatomy 0.000 description 1
• 230000003647 oxidation Effects 0.000 description 1
• 238000007254 oxidation reaction Methods 0.000 description 1
• 102000002574 p38 Mitogen-Activated Protein Kinases Human genes 0.000 description 1
• 108010068338 p38 Mitogen-Activated Protein Kinases Proteins 0.000 description 1
• 239000006179 pH buffering agent Substances 0.000 description 1
• 230000000242 pagocytic effect Effects 0.000 description 1
• 238000007911 parenteral administration Methods 0.000 description 1
• 244000052769 pathogen Species 0.000 description 1
• 230000009054 pathological process Effects 0.000 description 1
• 230000006320 pegylation Effects 0.000 description 1
• 239000000813 peptide hormone Substances 0.000 description 1
• 230000007030 peptide scission Effects 0.000 description 1
• 239000000816 peptidomimetic Substances 0.000 description 1
• 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 1
• 210000001322 periplasm Anatomy 0.000 description 1
• 238000002823 phage display Methods 0.000 description 1
• 230000008782 phagocytosis Effects 0.000 description 1
• 235000021317 phosphate Nutrition 0.000 description 1
• 150000003905 phosphatidylinositols Chemical class 0.000 description 1
• 229940080469 phosphocellulose Drugs 0.000 description 1
• 150000008300 phosphoramidites Chemical class 0.000 description 1
• 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
• 230000026731 phosphorylation Effects 0.000 description 1
• 238000006366 phosphorylation reaction Methods 0.000 description 1
• 230000035479 physiological effects, processes and functions Effects 0.000 description 1
• 230000035790 physiological processes and functions Effects 0.000 description 1
• 239000006187 pill Substances 0.000 description 1
• 230000036470 plasma concentration Effects 0.000 description 1
• 239000013600 plasmid vector Substances 0.000 description 1
• 108010065506 platelet factor V Proteins 0.000 description 1
• 229920000747 poly(lactic acid) Polymers 0.000 description 1
• 229920000573 polyethylene Polymers 0.000 description 1
• 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
• 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
• 229920001282 polysaccharide Polymers 0.000 description 1
• 239000005017 polysaccharide Substances 0.000 description 1
• 230000004481 post-translational protein modification Effects 0.000 description 1
• 230000001242 postsynaptic effect Effects 0.000 description 1
• 230000032361 posttranscriptional gene silencing Effects 0.000 description 1
• 230000013823 prenylation Effects 0.000 description 1
• 108010074732 preproenkephalin Proteins 0.000 description 1
• 208000037920 primary disease Diseases 0.000 description 1
• RZIMNEGTIDYAGZ-HNSJZBNRSA-N pro-gastrin Chemical compound N([C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N1CCC[C@H]1C(=O)NCC(=O)NCC(=O)NCC(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC(O)=O)C(=O)NCC(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N1CCC[C@H]1C(=O)NCC(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](C)C(=O)NCC(=O)NCC(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(N)=O)C(=O)[C@@H]1CCC(=O)N1 RZIMNEGTIDYAGZ-HNSJZBNRSA-N 0.000 description 1
• 108010041071 proenkephalin Proteins 0.000 description 1
• 230000000750 progressive effect Effects 0.000 description 1
• 230000000770 proinflammatory effect Effects 0.000 description 1
• 230000001915 proofreading effect Effects 0.000 description 1
• 108010022328 proparathormone Proteins 0.000 description 1
• 230000000069 prophylactic effect Effects 0.000 description 1
• 108010001670 prosomatostatin Proteins 0.000 description 1
• GGYTXJNZMFRSLX-UHFFFAOYSA-N prosomatostatin Chemical compound C1CCC(C(=O)NC(CCCNC(N)=N)C(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NC(CCCCN)C(=O)NC(C)C(=O)NCC(=O)NC2C(NC(CCCCN)C(=O)NC(CC(N)=O)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C4=CC=CC=C4NC=3)C(=O)NC(CCCCN)C(=O)NC(C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(C(=O)NC(CO)C(=O)NC(CSSC2)C(O)=O)C(C)O)C(C)O)=O)N1C(=O)C(C)NC(=O)C(CCSC)NC(=O)C(C)NC(=O)C1CCCN1C(=O)C(CC(N)=O)NC(=O)C(CO)NC(=O)C(CC(N)=O)NC(=O)C(C)NC(=O)C(N)CO GGYTXJNZMFRSLX-UHFFFAOYSA-N 0.000 description 1
• 238000001742 protein purification Methods 0.000 description 1
• 210000001938 protoplast Anatomy 0.000 description 1
• 210000000449 purkinje cell Anatomy 0.000 description 1
• 229940043131 pyroglutamate Drugs 0.000 description 1
• 238000011002 quantification Methods 0.000 description 1
• 230000006340 racemization Effects 0.000 description 1
• 230000002285 radioactive effect Effects 0.000 description 1
• 238000002601 radiography Methods 0.000 description 1
• 238000011084 recovery Methods 0.000 description 1
• 230000008439 repair process Effects 0.000 description 1
• 230000003938 response to stress Effects 0.000 description 1
• 238000003757 reverse transcription PCR Methods 0.000 description 1
• 210000003705 ribosome Anatomy 0.000 description 1
• 238000007363 ring formation reaction Methods 0.000 description 1
• 210000003296 saliva Anatomy 0.000 description 1
• 235000019515 salmon Nutrition 0.000 description 1
• 239000012266 salt solution Substances 0.000 description 1
• 230000028327 secretion Effects 0.000 description 1
• 239000006152 selective media Substances 0.000 description 1
• 238000000926 separation method Methods 0.000 description 1
• 230000000405 serological effect Effects 0.000 description 1
• 239000012679 serum free medium Substances 0.000 description 1
• 230000035939 shock Effects 0.000 description 1
• 230000019491 signal transduction Effects 0.000 description 1
• 238000002741 site-directed mutagenesis Methods 0.000 description 1
• 201000010153 skin papilloma Diseases 0.000 description 1
• 239000002002 slurry Substances 0.000 description 1
• 239000001509 sodium citrate Substances 0.000 description 1
• 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
• 239000001488 sodium phosphate Substances 0.000 description 1
• 235000011008 sodium phosphates Nutrition 0.000 description 1
• 239000007790 solid phase Substances 0.000 description 1
• 239000002904 solvent Substances 0.000 description 1
• 210000001082 somatic cell Anatomy 0.000 description 1
• 238000004611 spectroscopical analysis Methods 0.000 description 1
• 230000010473 stable expression Effects 0.000 description 1
• 239000012192 staining solution Substances 0.000 description 1
• 230000000638 stimulation Effects 0.000 description 1
• 210000002784 stomach Anatomy 0.000 description 1
• 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
• 230000008093 supporting effect Effects 0.000 description 1
• 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 1
• 239000000375 suspending agent Substances 0.000 description 1
• 208000024891 symptom Diseases 0.000 description 1
• 230000000946 synaptic effect Effects 0.000 description 1
• 230000017960 syncytium formation Effects 0.000 description 1
• 239000006188 syrup Substances 0.000 description 1
• 235000020357 syrup Nutrition 0.000 description 1
• 239000003826 tablet Substances 0.000 description 1
• 239000002562 thickening agent Substances 0.000 description 1
• 230000034005 thiol-disulfide exchange Effects 0.000 description 1
• RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
• 229940113082 thymine Drugs 0.000 description 1
• 229960002175 thyroglobulin Drugs 0.000 description 1
• 238000012090 tissue culture technique Methods 0.000 description 1
• 230000000699 topical effect Effects 0.000 description 1
• 231100000419 toxicity Toxicity 0.000 description 1
• 230000001988 toxicity Effects 0.000 description 1
• 125000003508 trans-4-hydroxy-L-proline group Chemical group 0.000 description 1
• FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
• 210000003412 trans-golgi network Anatomy 0.000 description 1
• 238000010361 transduction Methods 0.000 description 1
• 230000026683 transduction Effects 0.000 description 1
• 229940099456 transforming growth factor beta 1 Drugs 0.000 description 1
• 230000001052 transient effect Effects 0.000 description 1
• 230000032258 transport Effects 0.000 description 1
• 238000011269 treatment regimen Methods 0.000 description 1
• QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
• UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
• 239000013638 trimer Substances 0.000 description 1
• HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
• 229940038773 trisodium citrate Drugs 0.000 description 1
• RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
• 238000010798 ubiquitination Methods 0.000 description 1
• 230000034512 ubiquitination Effects 0.000 description 1
• 238000011144 upstream manufacturing Methods 0.000 description 1
• DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
• 229940045145 uridine Drugs 0.000 description 1
• 210000002700 urine Anatomy 0.000 description 1
• 210000003741 urothelium Anatomy 0.000 description 1
• 238000002255 vaccination Methods 0.000 description 1
• 235000013311 vegetables Nutrition 0.000 description 1
• 208000029257 vision disease Diseases 0.000 description 1
• 238000009736 wetting Methods 0.000 description 1
• 239000000080 wetting agent Substances 0.000 description 1
• 210000005253 yeast cell Anatomy 0.000 description 1