CA2500757A1 - Methode de criblage pour un polypeptide antimicrobien - Google Patents

Methode de criblage pour un polypeptide antimicrobien Download PDF

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Publication number
CA2500757A1
CA2500757A1 CA002500757A CA2500757A CA2500757A1 CA 2500757 A1 CA2500757 A1 CA 2500757A1 CA 002500757 A CA002500757 A CA 002500757A CA 2500757 A CA2500757 A CA 2500757A CA 2500757 A1 CA2500757 A1 CA 2500757A1
Authority
CA
Canada
Prior art keywords
cells
cell
antimicrobial
indicator
host
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002500757A
Other languages
English (en)
Inventor
Dorotea Raventos Segura
Anja Lykke Hansen
Per Holse Mygind
Hans-Henrik Kristensen Hogenhaug
Ida Ellingsgaard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2500757A1 publication Critical patent/CA2500757A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne une méthode de criblage d'un polynucléotide codant un peptide antimicrobien (AMP) pour son activité antimicrobienne. Le polynucléotique est introduit dans une cellule hôte sous la commande d'un promoteur inductible. Le fait de permettre à des cellules hôtes, sensibles à l'AMP, de croître en l'absence d'AMP avant l'ajout de l'inducteur fait que suffisamment de cellules hôtes sont présentes pour produire suffisamment d'AMP, au lieu d'éliminer ou d'inhiber précocement des cellules hôtes en les cultivant dès le départ en présence d'AMP. Après l'ajout de l'inducteur et l'expression de l'AMP dans la cellule hôte, la cellule hôte est cultivée en présence de cellules indicatrices. Les cellules hôtes pouvant réduire la prolifération de cellules indicatrices sont sélectionnées. Le polynucléotide codant un AMP est introduit seulement dans un seul type de cellules hôte, mais l'activité de l'AMP vers différentes cellules indicatrices peut être testée sans qu'il soit nécessaire d'avoir recours à l'introduction du polynucléotide dans plusieurs cellules hôtes.
CA002500757A 2002-10-09 2003-10-08 Methode de criblage pour un polypeptide antimicrobien Abandoned CA2500757A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200201518 2002-10-09
DKPA200201518 2002-10-09
DKPA200201854 2002-12-02
DKPA200201854 2002-12-02
PCT/DK2003/000669 WO2004033715A1 (fr) 2002-10-09 2003-10-08 Methode de criblage pour un polypeptide antimicrobien

Publications (1)

Publication Number Publication Date
CA2500757A1 true CA2500757A1 (fr) 2004-04-22

Family

ID=32094935

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002500757A Abandoned CA2500757A1 (fr) 2002-10-09 2003-10-08 Methode de criblage pour un polypeptide antimicrobien

Country Status (4)

Country Link
EP (1) EP1551993A1 (fr)
AU (1) AU2003269851A1 (fr)
CA (1) CA2500757A1 (fr)
WO (1) WO2004033715A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7148404B2 (en) * 2004-05-04 2006-12-12 Novozymes A/S Antimicrobial polypeptides
WO2006050611A1 (fr) * 2004-11-12 2006-05-18 The University Of British Columbia Peptides antimicrobiens
CA2593872A1 (fr) 2004-12-23 2006-06-29 Novozymes A/S Sequences de commande reagissant a des amp et leurs utilisations
CA2981705A1 (fr) * 2015-03-23 2016-09-29 Riptide Bioscience, Inc. Peptides antimicrobiens et leurs methodes d'utilisations
US10413584B1 (en) 2018-08-29 2019-09-17 Riptide Bioscience, Inc. Peptides having immunomodulatory properties
US10548944B1 (en) 2018-10-19 2020-02-04 Riptide Bioscience, Inc. Antimicrobial peptides and methods of using the same

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1707497A (en) * 1996-01-23 1997-08-20 Rigel Pharmaceuticals, Inc. Methods for screening for transdominant intracellular effector peptides and rna molecules
EP1187913A1 (fr) * 1999-05-31 2002-03-20 Novozymes A/S Procede de criblage peptidique
WO2002006517A2 (fr) * 2000-07-19 2002-01-24 Kairos Scientific, Inc. Methodes a haut rendement permettant de preparer et de cribler des composes qui affectent la viabilite cellulaire
DK1448595T3 (da) * 2001-11-20 2007-02-19 Novozymes As Antimikrobielle polypeptider

Also Published As

Publication number Publication date
WO2004033715A1 (fr) 2004-04-22
EP1551993A1 (fr) 2005-07-13
AU2003269851A1 (en) 2004-05-04

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