CA2468142C - Method of mass spectrometry - Google Patents
Method of mass spectrometry Download PDFInfo
- Publication number
- CA2468142C CA2468142C CA2468142A CA2468142A CA2468142C CA 2468142 C CA2468142 C CA 2468142C CA 2468142 A CA2468142 A CA 2468142A CA 2468142 A CA2468142 A CA 2468142A CA 2468142 C CA2468142 C CA 2468142C
- Authority
- CA
- Canada
- Prior art keywords
- mbar
- ions
- charges
- mass
- ion guide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 44
- 238000004949 mass spectrometry Methods 0.000 title claims abstract description 12
- 150000002500 ions Chemical class 0.000 claims abstract description 570
- 238000005040 ion trap Methods 0.000 claims abstract description 25
- 239000012491 analyte Substances 0.000 claims abstract description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 17
- 239000012634 fragment Substances 0.000 claims description 15
- 102000004169 proteins and genes Human genes 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 13
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 13
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 claims description 10
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 10
- 230000005405 multipole Effects 0.000 claims description 7
- 238000000132 electrospray ionisation Methods 0.000 claims description 5
- 230000002708 enhancing effect Effects 0.000 claims description 4
- 238000010265 fast atom bombardment Methods 0.000 claims description 4
- 238000009616 inductively coupled plasma Methods 0.000 claims description 4
- 238000001698 laser desorption ionisation Methods 0.000 claims description 4
- 230000000717 retained effect Effects 0.000 claims description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108010015899 Glycopeptides Proteins 0.000 claims description 3
- 102000002068 Glycopeptides Human genes 0.000 claims description 3
- 102000003886 Glycoproteins Human genes 0.000 claims description 3
- 108090000288 Glycoproteins Proteins 0.000 claims description 3
- 102000006382 Ribonucleases Human genes 0.000 claims description 3
- 108010083644 Ribonucleases Proteins 0.000 claims description 3
- 235000001014 amino acid Nutrition 0.000 claims description 3
- 150000001413 amino acids Chemical class 0.000 claims description 3
- 229920001222 biopolymer Polymers 0.000 claims description 3
- 150000001720 carbohydrates Chemical class 0.000 claims description 3
- 235000014633 carbohydrates Nutrition 0.000 claims description 3
- 239000002299 complementary DNA Substances 0.000 claims description 3
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 3
- 229930195729 fatty acid Natural products 0.000 claims description 3
- 239000000194 fatty acid Substances 0.000 claims description 3
- 150000004665 fatty acids Chemical class 0.000 claims description 3
- 150000004676 glycans Chemical class 0.000 claims description 3
- 239000005556 hormone Substances 0.000 claims description 3
- 229940088597 hormone Drugs 0.000 claims description 3
- 150000002632 lipids Chemical class 0.000 claims description 3
- 108020004999 messenger RNA Proteins 0.000 claims description 3
- 239000002207 metabolite Substances 0.000 claims description 3
- 229920001184 polypeptide Polymers 0.000 claims description 3
- 229920001282 polysaccharide Polymers 0.000 claims description 3
- 239000005017 polysaccharide Substances 0.000 claims description 3
- 150000003431 steroids Chemical class 0.000 claims description 3
- 235000000346 sugar Nutrition 0.000 claims description 3
- 150000008163 sugars Chemical class 0.000 claims description 3
- 239000011782 vitamin Substances 0.000 claims description 3
- 229940088594 vitamin Drugs 0.000 claims description 3
- 229930003231 vitamin Natural products 0.000 claims description 3
- 235000013343 vitamin Nutrition 0.000 claims description 3
- 102100022704 Amyloid-beta precursor protein Human genes 0.000 claims description 2
- 238000004252 FT/ICR mass spectrometry Methods 0.000 claims description 2
- 101000823051 Homo sapiens Amyloid-beta precursor protein Proteins 0.000 claims description 2
- DZHSAHHDTRWUTF-SIQRNXPUSA-N amyloid-beta polypeptide 42 Chemical compound C([C@@H](C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)NCC(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(O)=O)[C@@H](C)CC)C(C)C)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC(O)=O)C(C)C)C(C)C)C1=CC=CC=C1 DZHSAHHDTRWUTF-SIQRNXPUSA-N 0.000 claims description 2
- 238000000065 atmospheric pressure chemical ionisation Methods 0.000 claims description 2
- 238000000451 chemical ionisation Methods 0.000 claims description 2
- 238000001816 cooling Methods 0.000 claims description 2
- 238000003795 desorption Methods 0.000 claims description 2
- 238000004992 fast atom bombardment mass spectroscopy Methods 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 239000011159 matrix material Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 25
- 238000004458 analytical method Methods 0.000 abstract description 4
- 108010022337 Leucine Enkephalin Proteins 0.000 description 45
- URLZCHNOLZSCCA-UHFFFAOYSA-N leu-enkephalin Chemical compound C=1C=C(O)C=CC=1CC(N)C(=O)NCC(=O)NCC(=O)NC(C(=O)NC(CC(C)C)C(O)=O)CC1=CC=CC=C1 URLZCHNOLZSCCA-UHFFFAOYSA-N 0.000 description 45
- 102000004881 Angiotensinogen Human genes 0.000 description 22
- 108090001067 Angiotensinogen Proteins 0.000 description 22
- 238000001819 mass spectrum Methods 0.000 description 22
- 238000005086 pumping Methods 0.000 description 12
- 230000001965 increasing effect Effects 0.000 description 7
- 238000011144 upstream manufacturing Methods 0.000 description 7
- 230000035945 sensitivity Effects 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 238000005381 potential energy Methods 0.000 description 4
- 230000001133 acceleration Effects 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000004885 tandem mass spectrometry Methods 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 2
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 101100204059 Caenorhabditis elegans trap-2 gene Proteins 0.000 description 1
- 102000036675 Myoglobin Human genes 0.000 description 1
- 108010062374 Myoglobin Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 101800001442 Peptide pr Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 108010026552 Proteome Proteins 0.000 description 1
- 241000149788 Pseudophryne major Species 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000002330 electrospray ionisation mass spectrometry Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/06—Electron- or ion-optical arrangements
- H01J49/062—Ion guides
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Electron Tubes For Measurement (AREA)
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0129693A GB0129693D0 (en) | 2001-12-12 | 2001-12-12 | Mass spectrometer |
| GB0129693.8 | 2001-12-21 | ||
| GB0215626.3 | 2002-07-05 | ||
| GB0215626A GB0215626D0 (en) | 2001-12-12 | 2002-07-05 | Mass spectrometer |
| GB0217217.9 | 2002-07-25 | ||
| GB0217217A GB0217217D0 (en) | 2001-12-12 | 2002-07-25 | Mass spectrometer |
| US40151702P | 2002-08-07 | 2002-08-07 | |
| US60/401,517 | 2002-08-07 | ||
| PCT/GB2002/005628 WO2003050843A2 (en) | 2001-12-12 | 2002-12-12 | Method of mass spectrometry |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2468142A1 CA2468142A1 (en) | 2003-06-19 |
| CA2468142C true CA2468142C (en) | 2011-05-17 |
Family
ID=27448008
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2468142A Expired - Fee Related CA2468142C (en) | 2001-12-12 | 2002-12-12 | Method of mass spectrometry |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP1454337B1 (de) |
| CA (1) | CA2468142C (de) |
| WO (1) | WO2003050843A2 (de) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US12089932B2 (en) | 2018-06-05 | 2024-09-17 | Trace Matters Scientific Llc | Apparatus, system, and method for transferring ions |
| US11114290B1 (en) | 2020-05-07 | 2021-09-07 | Thermo Finnigan Llc | Ion funnels and systems incorporating ion funnels |
| US11581179B2 (en) | 2020-05-07 | 2023-02-14 | Thermo Finnigan Llc | Ion funnels and systems incorporating ion funnels |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19511333C1 (de) * | 1995-03-28 | 1996-08-08 | Bruker Franzen Analytik Gmbh | Verfahren und Vorrichtung für orthogonalen Einschuß von Ionen in ein Flugzeit-Massenspektrometer |
| US7041967B2 (en) * | 2001-05-25 | 2006-05-09 | Mds Inc. | Method of mass spectrometry, to enhance separation of ions with different charges |
-
2002
- 2002-12-12 CA CA2468142A patent/CA2468142C/en not_active Expired - Fee Related
- 2002-12-12 WO PCT/GB2002/005628 patent/WO2003050843A2/en not_active Ceased
- 2002-12-12 EP EP02785654.1A patent/EP1454337B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CA2468142A1 (en) | 2003-06-19 |
| EP1454337A2 (de) | 2004-09-08 |
| WO2003050843A2 (en) | 2003-06-19 |
| EP1454337B1 (de) | 2015-12-02 |
| WO2003050843A3 (en) | 2003-10-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20210831 |
|
| MKLA | Lapsed |
Effective date: 20191212 |