CA2436778A1 - Floral development genes - Google Patents

Floral development genes Download PDF

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CA2436778A1
CA2436778A1 CA002436778A CA2436778A CA2436778A1 CA 2436778 A1 CA2436778 A1 CA 2436778A1 CA 002436778 A CA002436778 A CA 002436778A CA 2436778 A CA2436778 A CA 2436778A CA 2436778 A1 CA2436778 A1 CA 2436778A1
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val
arg
leu
pro
gly
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Edgar B. Cahoon
Rebecca E. Cahoon
Theodore M. Klein
J. Antoni Rafalski
Hajime Sakai
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EIDP Inc
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

This invention relates to an isolated nucleic acid fragment encoding a flora l development proteins, more specifically a FT or Ap3 homolog. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the floral development proteins, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the FT or Ap3 homolog in a transformed host cell.

Description

TITLE
FLORAL DEVELOPMENT GENES
This application claims the benefit of U.S. Provisional Application No. 60/253,415, filed November 28, 2000, the entire contents of which are hereby incorporated by reference.
FIELD OF THE INVENTION
This invention is in the field of plant molecular biology. More specifically, this invention pertains to nucleic acid fragments encoding floral development proteins in plants and seeds.
BACKGROUND OF THE INVENTION
Flowering in plants is a consequence of the transition of the shoot apex from vegetative to reproductive growth in response to environmental and internal signals.
Currently, there is little information about how plants coordinate the activities of the cells that give rise to reproductive plant tissues, however, research has focused on identifying the genes that control this developmental process. Floral homeotic genes that control the specification of meristem and organ identity in developing flowers have been identified in Arabidopsis thaliana and Antirrhinum majus.
Most of these genes belong to a large family of regulatory genes that possess a characteristic DNA binding domain known as the MADS-box. Members of this gene family display primarily floral-specific expression and are homologous to transcription factors found in several animal and fungal species. Molecular evolutionary analysis reveal that there are appreciable differences in the substitution rates between different domains of these plant MADS-box genes. Phylogenetic analysis also demonstrate that members of the plant MADS-box gene family are organized into several distinct gene groups: the AGAMOUS, APETALA3 (Ap3) /PISTILLATA and APETALA1/AGL9 groups. Several genes that belong to the APETALA3 (Ap3) group have been identified in Arabidopsis thaliana (Jack et al., (1992) Cell. 68:683-697). Genes of this group have been shown to play a role in the control of organ identity of petals and stamens during floral development (Bowman et al., (1989) Plant Cell 7:37-52 and Bowman et al., (1991) Development 772:1-20;
Weigel and Meyerowitz (1994) Cell 78:203-209; Coen and Meyerowitz (1991) Nature 353:31-37; WO 93/21322). Thus, the shared evolutionary history of members of a gene group appear to reflect the distinct functional roles these MAD-box genes play in flower development.
The flowering locus T gene (FT) encodes a protein that appears to be involved in the regulating plant growth by controling the rate at which maturation occurs. For example, an increase in FT function has been shown to produce early flowering (Kardailsky et al., (1999) Science 286:1962-1965). Thus the FT gene may be useful to accelerate flowering in various crops.
The deduced sequence of the FT protein is similar to the sequence of TERMINAL FLOWER 1 (TFL1) and shares sequence similarity with membrane-s associated mammalian proteins (Kardailsky et al., (1999) Science 286:1962-1965).
TFL1 in Arabidopsis, and the homologous Antirrhinum gene CENTRORADIALIS
(CEN) play a key role in determining inflorescence architecture (Bradley et al.
(1997) Science 275:80-83; WO 97/10339; WO 99/53070).
There is a great deal of interest in identifying the genes that encode proteins involved in cellular differentiation in plants. These genes may be used in plant cells to control development. Accordingly, the availability of nucleic acid sequences encoding all or a portion of an Ap3 or FT or TFL1 gene homolog would facilitate studies to better understanding development in plants and provide genetic tools to enhance or otherwise alter plant developmental processes. Nucleic acid fragments encoding Ap3 homologs may be useful for engineering plant sterility/fertility, and flower development and morphology. Nucleic acid fragments encoding FT or TFL1 homologs may be useful for engineering flowering time, plant growth rate, inflorescence architecture, and tissue culture morphology and rate of cell division to enhance transformation.
SUMMARY OF THE INVENTION
The present invention concerns isolated polynucleotides comprising a nucleotide sequence encoding a polypeptide having FT or Ap3 homolog activity wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID N0:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 54, 56, 58, or 60 have at least 80% sequence identity.
It is preferred that the identity be at least 85%, it is preferable if the identity is at least 90%, it is more preferred that the identity be at least 95%. The present invention also relates to isolated polynucleotides comprising the complement of the nucleotide sequence, wherein the complement and the nucleotide sequence contain the same number of nucleotides and are 100% complementary. More specifically, the present invention concerns isolated polynucleotides encoding the polypeptide sequence of SEQ ID N0:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 54, 56, 58, or 60 or nucleotide sequences comprising the nucleotide sequence of SEQ ID N0:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 37, 39, 41, 43, 45, 47, 49, 53, 55, 57, or 59.
In a first embodiment, the present invention concerns an isolated polynucleotide comprising: (a) a nucleotide sequence encoding a polypeptide comprising at least 50, 100, 150, 160, 170, 175, or 200, amino acids, wherein the amino acid sequence of the polypeptide and the amino acid sequence of Of SEQ
ID
N0:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 54, 56, 58, or 60 have at least 80%, 85%, 90%, or 95% identity based on the Clustal alignment method, or (b) the complement of the nucleotide sequence, wherein the complement and the nucleotide sequence contain the same number of nucleotides and are 100% complementary. The polypeptide preferably comprises the amino acid sequence of Of SEQ ID N0:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 54, 56, 58, or 60. The nucleotide sequence preferably comprises the nucleotide sequence of SEQ ID N0:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 37, 39, 41, 43, 45, 47, 49, 53, 55, 57, or 59. The polypeptide preferably is a FT or Ap3 homolog.
In a second embodiment, the present invention relates to a recombinant DNA
construct comprising any of the isolated polynucleotides of the present invention operably linked to a regulatory sequence, and a cell, a plant, and a seed comprising the recombinant DNA construct.
In a third embodiment, the present invention relates to a vector comprising any of the isolated polynucleotides of the present invention.
In a fourth embodiment, the present invention relates to an isolated polynucleotide comprising a nucleotide sequence comprised by any of the polynucleotides of the first embodiment, wherein the nucleotide sequence contains at least 30, 40, 50, 60, 100, 150, 160, 170, 175, or 200 nucleotides.
In a fifth embodiment, the present invention relates to a method for transforming a cell comprising transforming a cell with any of the isolated polynucleotides of the present invention, and the cell transformed by this method.
Advantageously, the cell is eukaryotic, e.g., a yeast or plant cell, or prokaryotic, e.g., a bacterium.
In a sixth embodiment, the present invention relates to a method for producing a transgenic plant comprising transforming a plant cell with any of the isolated polynucleotides of the present invention and regenerating a plant from the transformed plant cell. The invention also concerns the transgenic plant produced by this method, and the seed obtained from this transgenic plant.
In a seventh embodiment, the present invention concerns an isolated polypeptide comprising an amino acid sequence comprising at least 50, 100, 150, 160, 170, 175, or 200, amino acids, wherein the amino acid sequence and the amino acid sequence of SEQ ID N0:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 54, 56, 58, 'or 60 have at least 80%, 85%, 90%, or 95% identity based on the Clustal alignment method. The amino acid sequence preferably comprises the amino acid sequence of SEQ ID N0:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 54, 56, 58, or 60. The polypeptide preferably is a FT or Ap3 homolog.
In an eight embodiment, the invention concerns a method for isolating a polypeptide encoded by the polynucleotide of the present invention comprising isolating the polypeptide from a cell containing a recombinant DNA construct comprising the polynucleotide operably linked to a regulatory sequence.
In a ninth embodiment, the present invention relates to a virus, preferably a baculovirus, comprising any of the isolated polynucleotides of the present invention or any of the recombinant DNA constructs of the present invention.
In a tenth embodiment, the invention relates to a method of selecting an isolated polynucleotide that affects the level of expression of a gene encoding a FT
or Ap3 homolog protein or activity in a host cell, preferably a plant cell, the method comprising the steps of: (a) constructing an isolated polynucleotide of the present invention or an isolated recombinant DNA construct of the present invention;
(b) introducing the isolated polynucleotide or the isolated recombinant DNA
construct into a host cell; (c) measuring the level of the FT or Ap3 homolog protein or activity in the host cell containing the isolated polynucleotide; and (d) comparing the level of the FT or Ap3 homolog protein or activity in the host cell containing the isolated polynucleotide with the level of the FT or Ap3 homolog protein or activity in the host cell that does not contain the isolated polynucleotide.
In an eleventh embodiment, the invention concerns a method of obtaining a nucleic acid fragment encoding a substantial portion of a FT or Ap3 homolog protein, preferably a plant FT or Ap3 homolog protein comprising the steps of:
synthesizing an oligonucleotide primer comprising a nucleotide sequence of at least (preferably at least 40, most preferably at least 60) contiguous nucleotides derived from a nucleotide sequence selected from the group consisting of SEQ
ID
N0:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 37, 39, 41, 43, 45, 47, 30 49, 53, 55, 57, or 59, and the complement of such nucleotide sequences; and amplifying a nucleic acid fragment (preferably a cDNA inserted in a cloning vector) using the oligonucleotide primer. The amplified nucleic acid fragment preferably will encode a substantial portion of a FT or Ap3 homolog amino acid sequence.
In a twelfth embodiment, this invention relates to a method of obtaining a nucleic acid fragment encoding all or a substantial portion of the amino acid sequence encoding a FT or Ap3 homolog protein comprising the steps of: probing a cDNA or genomic library with an isolated polynucleotide of the present invention;
identifying a DNA clone that hybridizes with an isolated polynucleotide of the present invention; isolating the identified DNA clone; and sequencing the cDNA
or genomic fragment that comprises the isolated DNA clone.
In a thirteenth embodiment, this invention concerns a method for positive selection of a transformed cell comprising: (a) transforming a host cell with the recombinant DNA construct of the present invention or an expression cassette of the present invention; and (b) growing the transformed host cell, preferably a plant cell, such as a monocot or a dicot, under conditions which allow expression of the FT or Ap3 homolog polynucleotide in an amount sufficient to complement a null mutant, or a conditional null mutant, to provide a positive selection means.
In a fourteenth embodiment, this invention relates to a method of altering the level of expression of a FT or Ap3 homolog protein in a host cell comprising:
(a) transforming a host cell with a recombinant DNA construct of the present invention; and (b) growing the transformed host cell under conditions that are suitable for expression of the recombinant DNA construct wherein expression of the recombinant DNA construct results in production of altered levels of the FT or Ap3 homolog protein in the transformed host cell.
BRIEF DESCRIPTION OF THE
DRAWINGS AND SEQUENCE LISTINGS
The invention can be more fully understood from the following detailed description and the accompanying drawings and Sequence Listing which form a part of this application.
Figure 1 depicts an alignment of amino acid sequences of FT homologs encoded by nucleotide sequences derived from a contig assembled from balsam pear clones fds.pk0003.h2, fds.pk0026.d10, and fds1n.pk001.p18 (SEQ ID N0:4), garden balsam clone ids.pk0031.a5 (SEQ ID N0:6), contig assembled from corn clones cbn10.pk0052.f5, cbn2.pk0035.f12, cco1 n.pk0010.h3, p0095.cwsas14f, p0119.cmtmg45rb, and p0128.cpicl42r (SEQ ID N0:8), corn clone cc71se-b.pk0003.h10 (SEQ ID N0:10), corn clone cco1 n.pk0037.d10 (SEQ ID N0:12), contig assembled from corn clones cen3n.pk0004.e9, cen3n.pk0047.h7, cen3n.pk0093.f1, cen3n.pk0165.f1, and p0120.cdeae63r (SEQ ID N0:14), corn clone p0014.ctush42r (SEQ ID N0:16), corn clone p0081.chcad07r (SEQ ID
NO:18), corn clone p0104.cabak14rb (SEQ ID N0:20), corn clone p0118.chsaq04rb (SEQ ID N0:22), rice clone rIs24.pk0017.c7 (SEQ ID N0:30), rice clone rr1.pk0043.f9 (SEQ ID N0:32), contig assembled from soybean clones se3.pk0036.g4 and se6.pk0039.h6 (SEQ ID N0:36), soybean clone srr2c.pk002.o7 (SEQ ID N0:38), contig assembled from soybean clone ssLpk0007.a9 and a PCR
fragment sequence ( SEQ ID N0:40), wheat clone wdk2c.pk012.o17 (SEQ ID
N0:42), and wheat clone wdk9n1.pk001.020 (SEQ ID N0:44) and Oryza sativa (NCBI GI No. 5360178; SEQ ID N0:51). Amino acids which are conserved among all and at least two sequences with an amino acid at that position are indicated with an asterisk (*). Dashes are used by the program to maximize alignment of the sequences.
Figure 2 depicts an alignment of amino acid sequences of Ap3 homologs encoded by nucleotide sequences derived from corn clone cta1 n.pk0050.f8 (SEQ
ID N0:46), corn clone ctn1c.pk002.j23 (SEQ ID N0:48), soybean clone IO sfi1n.pk001.116 (SEQ ID N0:50), and Oryza sativa (NCBI GI No. 5295980; SEQ
ID
N0:52). Amino acids which are conserved among all and at least two sequences with an amino acid at that position are indicated with an asterisk (*). Dashes are used by the program to maximize alignment of the sequences.
Table 1 lists the polypeptides that are described herein, the designation of the cDNA clones that comprise the nucleic acid fragments encoding polypeptides representing all or a substantial portion of these polypeptides, and the corresponding identifier (SEQ ID NO:) as used in the attached Sequence Listing.
The sequence descriptions and Sequence Listing attached hereto comply with the rules governing nucleotide and/or amino acid sequence disclosures in patent applications as set forth in 37 C.F.R. ~1.821-1.825.

Floral Development Proteins SEQ ID NO:
Protein Plant Source Clone Desi nation Nucleotide Amino Acid ........_.............._................................._... ~... ) . ..
.._........_ ..._....._........___........._........................_ .._........
.....__........_..._....._........._.__..._............_g......................
........_............_. .._ . .. . .. .. _....( .....~
.................................?......_........_.~..............._._....._...
.................._...?...........
FT Homolog (Peruvian Lily) eal1 c.pk006.e6 1 2 FT Homolog (Balsam Pear) Contig of 3 4 fds.pk0003.h2 fds.pk0026.d 10 fds1 n.pk001.p18 FT Homolog (Garden ids.pk0031.a5 5 6 Balsam) FT Homolog (Corn) Contig of 7 8 cbn10.pk0052.f5 cbn2.pk0035.f12 cco1 n.pk001 O.h3 p0095.cwsas14f p0119.cmtmg45rb p0128.cpicl42r SEQ ID NO:

__.T. Protein (Plant.Sou.rce)"-~".Clone..-Desig,nation.....~,T, (Nucleotide)"
.-Amino ""- _ Acid ..._.__..........._... _...._...._....._.._.___._.__ ~.__.______...__._...._.~..........

FT Homolog (Corn) cc71se-b.pk0003.h109 10 FT Homolog (Corn) cco1n.pk0037.d10 11 12 FT Homolog (Corn) Contig of 13 14 cen3n.pk0004.e9 cen3n.pk0047.h7 cen3n.pk0093.f1 cen3n.pk0165.f1 p0120.cdeae63r FT Homolog (Corn) p0014.ctush42r 15 16 FT Homolog (Corn) p0081.chcad07r 17 18 FT Homolog (Corn) p0104.cabak14rb 19 20 FT Homolog (Corn) p0118.chsaq04rb 21 22 FT Homolog (Rice) rbm1c.pk001.a6 23 24 FT Homolog (Rice) Contig of 25 26 rl0n.pk0022.h10 rl0n.pk0022.h11 FT Homolog (Rice) rIr48.pk0001.b1 27 28 FT Homolog (Rice) rIs24.pk0017.c7 29 30 FT Homolog (Rice) rr1.pk0043.f9 31 32 FT Homolog (Rice) rsr9n.pk001.d1 33 34 FT Homolog (Soybean) Contig of 35 36 ~ .

se3.pk0036.g4 se6.pk0039.h6 FT Homolog (Soybean) srr2c.pk002.o7 37 38 FT Homolog (Soybean) Contig of 39 40 .

ssLpk0007.a9 PCR fragment sequence FT Homolog (Wheat) wdk2c.pk012.o17 41 42 FT Homolog (Wheat) wdk9n1.pk001.o20 43 44 Ap3 Homolog (Corn) cta1 n.pk0050.f8 45 46 Ap3 Homolog (Corn) ctn1c.pk002.j23 47 48 Ap3 Homolog (Soybean) sfl1 n.pk001.116 49 50 FT Homolog (Corn) cta1 n.pk0058.d11 53 54 b FT Homolog (Rice) rbm1c.pk001.a6:fis55 56 FT Homolog (Rice) rl0n.pk0022.h10:fis57 58 FT Homolog (Rice) rsr9n.pk001.d1:fis59 60 The Sequence Listing contains the one letter code for nucleotide sequence characters and the three letter codes for amino acids as defined in conformity with the IUPAC-IUBMB standards described in Nucleic Acids Res. 13:3021=3030 (1985) and in the Biochemical J. 219 (No. 2):345-373 (1984) which are herein incorporated by reference. The symbols and format used for nucleotide and amino acid sequence data comply with the rules set forth in 37 C.F.R. ~1.822.
DETAILED DESCRIPTION OF THE INVENTION
In the context of this disclosure, a number of terms shall be utilized. The terms "polynucleotide", "polynucleotide sequence", "nucleic acid sequence", and "nucleic acid fragment"/"isolated nucleic acid fragment" are used interchangeably herein. These terms encompass nucleotide sequences and the like. A
polynucleotide may be a polymer of RNA or DNA that is single- or double-stranded, that optionally contains synthetic, non-natural or altered nucleotide bases. A
polynucleotide in the form of a polymer of DNA may be comprised of one or more segments of cDNA, genomic DNA, synthetic DNA, or mixtures thereof. An isolated polynucleotide of the present invention may include at least 30 contiguous nucleotides, preferably at least 40 contiguous nucleotides, most preferably at least 60 contiguous nucleotides derived from SEQ ID N0:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 37, 39, 41, 43, 45, 47, 49, 53, 55, 57, or 59, or the complement of such sequences.
The term "isolated" refers to materials, such as nucleic acid molecules and/or proteins, which are substantially free or otherwise removed from components that normally accompany or interact with the materials in a naturally occurring environment. Isolated polynucleotides may be purified from a host cell in which they naturally occur. Conventional nucleic acid purification methods known to skilled artisans may be used to obtain isolated polynucleotides. The term also embraces recombinant polynucleotides and chemically synthesized polynucleotides.
The term "recombinant" means, for example, that a nucleic acid sequence is made by an artificial combination of two otherwise separated segments of sequence, e.g., by chemical synthesis or by the manipulation of isolated nucleic acids by genetic engineering techniques.
As used herein, "contig" refers to a nucleotide sequence that is assembled from two or more constituent nucleotide sequences that share common or overlapping regions of sequence homology. For example, the nucleotide sequences of two or more nucleic acid fragments can be compared and aligned in order to identify common or overlapping sequences. Where common or overlapping sequences exist between two or more nucleic acid fragments, the sequences (and thus their corresponding nucleic acid fragments) can be assembled into a single contiguous nucleotide sequence.
As used herein, "substantially similar" refers to nucleic acid fragments wherein changes in one or more nucleotide bases results in substitution of one or more amino acids, but do not affect the functional properties of the polypeptide encoded by the nucleotide sequence. "Substantially similar" also refers to nucleic acid fragments wherein changes in one or more nucleotide bases does not affect the ability of the nucleic acid fragment to mediate alteration of gene expression by gene silencing through for example antisense or co-suppression technology.
"Substantially similar" also refers to modifications of the nucleic acid fragments of the instant invention such as deletion or insertion of one or more nucleotides that do not substantially affect the functional properties of the resulting transcript vis-a-vis the ability to mediate gene silencing or alteration of the functional properties of the resulting protein molecule. It is therefore understood that the invention encompasses more than the specific exemplary nucleotide or amino acid sequences and includes functional equivalents thereof. The terms "substantially similar" and "corresponding substantially" are used interchangeably herein.
Substantially similar nucleic acid fragments may be selected by screening nucleic acid fragments representing subfragments or modifications of the nucleic acid fragments of the instant invention, wherein one or more nucleotides are substituted, deleted and/or inserted, for their ability to affect the level of the polypeptide encoded by the unmodified nucleic acid fragment in a plant or plant cell.
For example, a substantially similar nucleic acid fragment representing at least 30 contiguous nucleotides, preferably at least 40 contiguous nucleotides, most preferably at least 60 contiguous nucleotides derived from the instant nucleic acid fragment can be constructed and introduced into a plant or plant cell. The level of the polypeptide encoded by the unmodified nucleic acid fragment present in a plant or plant cell exposed to the substantially similar nucleic fragment can then be compared to the level of the polypeptide in a plant or plant cell that is not exposed to the substantially similar nucleic acid fragment.
For example, it is well known in the art that antisense suppression and co-suppression of gene expression may be accomplished using nucleic acid fragments representing less than the entire coding region of a gene, and by using nucleic acid fragments that do not share 100% sequence identity with the gene to be suppressed. Moreover, alterations in a nucleic acid fragment which result in the production of a chemically equivalent amino acid at a given site, but do not effect the functional properties of the encoded polypeptide, are well known in the art.
Thus, a codon for the amino acid alanine, a hydrophobic amino acid, may be substituted by a codon encoding another less hydrophobic residue, such as glycine, or a more hydrophobic residue, such as valine, leucine, or isoleucine.
Similarly, changes which result in substitution of one negatively charged residue for another, such as aspartic acid for glutamic acid, or one positively charged residue for another, such as lysine for arginine, can also be expected to produce a functionally equivalent product. Nucleotide changes which result in alteration of the N-terminal and C-terminal portions of the polypeptide molecule would also not be expected to alter the activity of the polypeptide. Each of the proposed modifications is well within the routine skill in the art, as is determination of retention of biological activity of the encoded products. Consequently, an isolated polynucleotide comprising a nucleotide sequence of at least 30 (preferably at least 40, most preferably at least 60) contiguous nucleotides derived from a nucleotide sequence selected from the group consisting of SEQ ID N0:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 37, 39, 41, 43, 45, 47, 49, 53, 55, 57, or 59, and the complement of such nucleotide sequences may be used to affect the expression and/or function of a FT
or Ap3 homolog in a host cell. A method of using an isolated polynucleotide to affect the level of expression of a polypeptide in a host cell (eukaryotic, such as plant or yeast, prokaryotic such as bacterial) may comprise the steps of:
constructing an isolated polynucleotide of the present invention or an isolated chimeric gene of the present invention; introducing the isolated polynucleotide or the isolated chimeric gene into a host~cell; measuring the level of a polypeptide or enzyme activity in the host cell containing the isolated polynucleotide; and comparing the level of a polypeptide or enzyme activity in the host cell containing the isolated polynucleotide with the level of a polypeptide or enzyme activity in a host cell that does not contain the isolated polynucleotide.
Moreover, substantially similar nucleic acid fragments may also be characterized by their ability to hybridize. Estimates of such homology are provided by either DNA-DNA or DNA-RNA hybridization under conditions of stringency as is well understood by those skilled in the art (Names and Higgins, Eds. (1985) Nucleic Acid Hybridisation, IRL Press, Oxford, U.K.). Stringency conditions can be adjusted to screen for moderately similar fragments, such as homologous sequences from distantly related organisms, to highly similar fragments, such as genes that duplicate functional enzymes from closely related organisms. Post-hybridization washes determine stringency conditions. One set of preferred conditions uses a series of washes starting with 6X SSC, 0.5% SDS at room temperature for 15 min, then repeated with 2X SSC, 0.5% SDS at 45°C for 30 min, and then repeated twice with 0.2X SSC, 0.5% SDS at 50°C for 30 min. A more preferred set of stringent conditions uses higher temperatures in which the washes are identical to those above except for the temperature of the final two 30 min washes in 0.2X SSC, 0.5%
SDS was increased to 60°C. Another preferred set of highly stringent conditions uses two final washes in 0.1X SSC, 0.1% SDS at 65°C.
Substantially similar nucleic acid fragments of the instant invention may also be characterized by the percent identity of the amino acid sequences that they encode to the amino acid sequences disclosed herein, as determined by algorithms commonly employed by those skilled in this art. Suitable nucleic acid fragments (isolated polynucleotides of the present invention) encode polypeptides that are at least about 70% identical, preferably at least about 80% identical to the amino acid sequences reported herein. Preferred nucleic acid fragments encode amino acid sequences that are at least about 85% identical to the amino acid sequences reported herein. More preferred nucleic acid fragments encode amino acid sequences that are at least about 90% identical to the amino acid sequences reported herein. Most preferred are nucleic acid fragments that encode amino acid sequences that are at least about 95% identical to the amino acid sequences reported herein. Suitable nucleic acid fragments not only have the above identities but typically encode a polypeptide having at least 50 amino acids, preferably at least 100 amino acids, more preferably at least 150 amino acids, still more preferably at least 200 amino acids, and most preferably at least 250 amino acids. Sequence alignments and percent identity calculations were performed using the Megalign program of the LASERGENE bioinformatics computing suite (DNASTAR Inc., Madison, WI). Multiple alignment of the sequences was performed using the Clustal method of alignment (Higgins and Sharp (1989) CABIOS. 5:151-153) with the default parameters (GAP PENALTY=10, GAP LENGTH PENALTY=10). Default parameters for pairwise alignments using the Clustal method were KTUPLE 1, GAP
PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5.
A "substantial portion" of an amino acid or nucleotide sequence comprises an amino acid or a nucleotide sequence that is sufficient to afford putative identification of the protein or gene that the amino acid or nucleotide sequence comprises.
Amino acid and nucleotide sequences can be evaluated either manually by one skilled in the art, or by using computer-based sequence comparison and identification tools that employ algorithms such as BLAST (Basic Local Alignment Search Tool; Altschul et al. (1993) J. Mol. Biol. 215:403-410; see also the explanation of the BLAST alogarithm on the world wide web site for the National Center for Biotechnology Information at the National Library of Medicine of the National Institutes of Health). In general, a sequence of ten or more contiguous amino acids or thirty or more contiguous nucleotides is necessary in order to putatively identify a polypeptide or nucleic acid sequence as homologous to a known protein or gene. Moreover, with respect to nucleotide sequences, gene-specific oligonucleotide probes comprising 30 or more contiguous nucleotides may be used in sequence-dependent methods of gene identification (e.g., Southern hybridization) and isolation (e.g., in situ hybridization of bacterial colonies or bacteriophage plaques). In addition, short oligonucleotides of 12 or more nucleotides may be used as amplification primers in PCR in order to obtain a particular nucleic acid fragment comprising the primers. Accordingly, a "substantial portion" of a nucleotide sequence comprises a nucleotide sequence that will afford specific identification andlor isolation of a nucleic acid fragment comprising the sequence. The instant specification teaches amino acid and nucleotide sequences encoding polypeptides that comprise one or more particular plant proteins. The skilled artisan, having the benefit of the sequences as reported herein, may now use all or a substantial portion of the disclosed sequences for purposes known to those skilled in this art. Accordingly, the instant invention comprises the complete sequences as reported in the accompanying Sequence Listing, as well as substantial portions of those sequences as defined above.
"Codon degeneracy" refers to divergence in the genetic code permitting variation of the nucleotide sequence without effecting the amino acid sequence of an encoded polypeptide. Accordingly, the instant invention relates to any nucleic acid fragment comprising a nucleotide sequence that encodes all or a substantial portion of the amino acid sequences set forth herein. The skilled artisan is well aware of the "codon-bias" exhibited by a specific host cell in usage of nucleotide codons to specify a given amino acid. Therefore, when synthesizing a nucleic acid fragment for improved expression in a host cell, it is desirable to design the nucleic acid fragment such that its frequency of codon usage approaches the frequency of preferred codon usage of the host cell.
"Synthetic nucleic acid fragments" can be assembled from oligonucleotide building blocks that are chemically synthesized using procedures known to those skilled in the art. These building blocks are ligated and annealed to form larger nucleic acid fragments which may then be enzymatically assembled to construct the entire desired nucleic acid fragment. "Chemically synthesized", as related to a nucleic acid fragment, means that the component nucleotides were assembled in vitro. Manual chemical synthesis of nucleic acid fragments may be accomplished using well established procedures, or automated chemical synthesis can be performed using one of a number of commercially available machines.
Accordingly, the nucleic acid fragments can be tailored for optimal gene expression based on optimization of the nucleotide sequence to reflect the codon bias of the host cell.
The skilled artisan appreciates the likelihood of successful gene expression if codon usage is biased towards those codons favored by the host. Determination of preferred codons can be based on a survey of genes derived from the host cell where sequence information is available.
"Gene" refers to a nucleic acid fragment that expresses a specific protein, including regulatory sequences preceding (5' non-coding sequences) and following (3' non-coding sequences) the coding sequence. "Native gene" refers to a gene as found in nature with its own regulatory sequences. "Chimeric gene" refers any gene that is not a native gene, comprising regulatory and coding sequences that are not found together in nature. Accordingly, a chimeric gene may comprise regulatory sequences and coding sequences that are derived from different sources, or regulatory sequences and coding sequences derived from the same source, but arranged in a manner different than that found in nature. "Endogenous gene"
refers to a native gene in its natural location in the genome of an organism. A
"foreign-gene" refers to a gene not normally found in the host organism, but that is introduced into the host organism by gene transfer. Foreign genes can comprise native genes inserted into a non-native organism, or chimeric genes. A
"transgene"
is a gene that has been introduced into the genome by a transformation procedure.
"Coding sequence" refers to a nucleotide sequence that codes for a specific amino acid sequence. "Regulatory sequences" refer to nucleotide sequences located upstream (5' non-coding sequences), within, or downstream (3' non-coding sequences) of a coding sequence, and which influence the transcription, RNA
processing or stability, or translation of the associated coding sequence.
Regulatory sequences may include promoters, translation leader sequences, introns, and polyadenylation recognition sequences.
"Promoter" refers to a nucleotide sequence capable of controlling the expression of a coding sequence or functional RNA. In general, a coding sequence is located 3' to a promoter sequence. The promoter sequence consists of proximal and more distal upstream elements, the latter elements often referred to as enhancers. Accordingly, an "enhancer" is a nucleotide sequence which can stimulate promoter activity and may be an innate element of the promoter or a heterologous element inserted to enhance the level or tissue-specificity of a promoter. Promoters may be derived in their entirety from a native gene, or may be composed of different elements derived from different promoters found in nature, or may even comprise synthetic nucleotide segments. It is understood by those skilled in the art that different promoters may direct the expression of a gene in different tissues or cell types, or at different stages of development, or in response to different environmental conditions. Promoters which cause a nucleic acid fragment to be expressed in most cell types at most times are commonly referred to as "constitutive promoters". New promoters of various types useful in plant cells are constantly being discovered; numerous examples may be found in the compilation by Okamuro and Goldberg (1989) Biochemistry of Plants 75:1-82. It is further recognized that since in most cases the exact boundaries of regulatory sequences have not been completely defined, nucleic acid fragments of different lengths may have identical promoter activity.
"Translation leader sequence" refers to a nucleotide sequence located between the promoter sequence of a gene and the coding sequence. The translation leader sequence is present in the fully processed mRNA upstream of the translation start sequence. The translation leader sequence may affect processing of the primary transcript to mRNA, mRNA stability or translation efficiency.
Examples of translation leader sequences have been described (Turner and Foster (1995) Mol. Biotechnol. 3:225-236).
"3' non-coding sequences" refer to nucleotide sequences located downstream of a coding sequence and include polyadenylation recognition sequences and other sequences encoding regulatory signals capable of affecting mRNA processing or gene expression. The polyadenylation signal is usually characterized by affecting the addition of polyadenylic acid tracts to the 3' end of the mRNA precursor. The use of different 3' non-coding sequences is exemplified by Ingelbrecht et al. (1989) Plant Cell 7:671-680.
"RNA transcript" refers to the product resulting from RNA polymerase-catalyzed transcription of a DNA sequence. When the RNA transcript is a perfect complementary copy of the DNA sequence, it is referred to as the primary transcript or it may be a RNA sequence derived from post-transcriptional processing of the primary transcript and is referred to as the mature RNA. "Messenger RNA
(mRNA)"
refers to the RNA that is without introns and that can be translated into polypeptides by the cell. "cDNA" refers to DNA that is complementary to and derived from an mRNA template. The cDNA can be single-stranded or converted to double stranded form using, for example, the Klenow fragment of DNA polymerase I.
"Sense-RNA" refers to an RNA transcript that includes the mRNA and so can be translated into a polypeptide by the cell. "Antisense RNA" refers to an RNA

transcript that is complementary to all or part of a target primary transcript or mRNA
and that blocks the expression of a target gene (see U.S. Patent No.
5,107,065, incorporated herein by reference). The complementarity of an antisense RNA may be with any part of the specific nucleotide sequence, i.e., at the 5' non-coding sequence, 3' non-coding sequence, introns, or the coding sequence. "Functional RNA" refers to sense RNA, antisense RNA, ribozyme RNA, or other RNA that may not be translated but yet has an effect on cellular processes.
The term "operably linked" refers to the association of two or more nucleic acid fragments on a single polynucleotide so that the function of one is affected by the other. For example, a promoter is operably linked with a coding sequence when it is capable of affecting the expression of that coding sequence (i.e., that the coding sequence is under the transcriptional control of the promoter). Coding sequences can be operably linked to regulatory sequences in sense or antisense orientation, The term "expression", as used herein, refers to the transcription and stable accumulation of sense (mRNA) or antisense RNA derived from the nucleic acid fragment of the invention. Expression may also refer to translation of mRNA
into a polypeptide. "Antisense inhibition" refers to the production of antisense RNA
transcripts capable of suppressing the expression of the target protein.
"Overexpression" refers to the production of a gene product in transgenic organisms that exceeds levels of production in normal or non-transformed organisms.
"Co-suppression" refers to the production of sense RNA transcripts capable of suppressing the expression of identical or substantially similar foreign or endogenous genes (U.S. Patent No. 5,231,020, incorporated herein by reference).
A "protein" or "polypeptide" is a chain of amino acids arranged in a specific order determined by the coding sequence in a polynucleotide encoding the polypeptide. Each protein or polypeptide has a unique function.
"Altered levels" or "altered expression" refers to the production of gene products) in transgenic organisms in amounts or proportions that differ from that of normal or non-transformed organisms.
"Mature protein" or the term "mature" when used in describing a protein refers to a post-translationally processed polypeptide; i.e., one from which any pre-or propeptides present in the primary translation product have been removed.
"Precursor protein" or the term "precursor" when used in describing a protein refers to the primary product of translation of mRNA; i.e., with pre- and propeptides still present. Pre- and propeptides may be but are not limited to intracellular localization signals.

A "chloropiast transit peptide" is an amino acid sequence which is translated in conjunction with a protein and directs the protein to the chloroplast or other plastid types present in the cell in which the protein is made. "Chloroplast transit sequence" refers to a nucleotide sequence that encodes a chloroplast transit peptide. A "signal peptide" is an amino acid sequence which is translated in conjunction with a protein and directs the protein to the secretory system (Chrispeels (1991 ) Ann. Rev. Plant Phys. Plant Mol. Biol. 42:21-53). If the protein is to be directed to a vacuole, a vacuolar targeting signal (supra) can further be added, or if to the endoplasmic reticulum, an endoplasmic reticulum retention signal (supra) may be added. If the protein is to be directed to the nucleus, any signal peptide present should be removed and instead a nuclear localization signal included (Raikhel (1992) Plant Phys. 100:1627-1632).
"Transformation" refers to the transfer of a nucleic acid fragment into the genome of a host organism, resulting in genetically stable inheritance. Host organisms containing the transformed nucleic acid fragments are referred to as "transgenic" organisms. Examples of methods of plant transformation include Agro,bacterium-mediated transformation (De Blaere et al. (1987) Meth. Enzymol.
143:277) and particle-accelerated or "gene gun" transformation technology (Klein et al. (1987) Nature (London) 327:70-73; U.S. Patent No. 4,945,050, incorporated herein by reference). Thus, isolated polynucleotides of the present invention can be incorporated into recombinant constructs, typically DNA constructs, capable of introduction into and replication in a host cell. Such a construct can be a vector that includes a replication system and sequences that are capable of transcription and translation of a polypeptide-encoding sequence in a given host cell. A number of vectors suitable for stable transfection of plant cells or for the establishment of transgenic plants have been described in, e.g., Pouwels et al., Cloning Vectors: A
Laboratory Manual, 1985, supp. 1987; Weissbach and Weissbach, Methods for Plant Molecular Biology, Academic Press, 1989; and Flevin et al., Plant Molecular Biology Manual, Kluwer Academic Publishers, 1990. Typically, planfi expression vectors include, for example, one or more cloned plant genes under the transcriptional control of 5' and 3' regulatory sequences and a dominant selectable marker. Such plant expression vectors also can contain a promoter regulatory region (e.g., a regulatory region controlling inducible or constitutive, environmentally- or developmentally-regulated, or cell- or tissue-specific expression), a transcription initiation start site, a ribosome binding site, an RNA
processing signal, a transcription termination site, and/or a polyadenylation signal.

Standard recombinant DNA and molecular cloning techniques used herein are well known in the art and are described more fully in Sambrook et al.
Molecular Cloning: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, 1989 (hereinafter "Maniatis"). ' "PCR" or "polymerase chain reaction" is well known by those skilled in the art as a technique used for the amplification of specific DNA segments (U.S.
Patent Nos. 4,683,195 and 4,800,159).
The present invention concerns an isolated polynucleotide comprising a nucleotide sequence encoding a FT or Ap3 homolog polypeptide having at least 80%, 85%, 90%, 95%, or 100% identity, based on the Clustal method of alignment, when compared to a polypeptide selected from the group consisting of SEQ ID
N0:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 54, 56, 58, or 60.
This invention also relates to the isolated complement of such polynucleotides, wherein the complement and the polynucleotide consist of the same number of nucleotides, and the nucleotide sequences of the complement and the polynucleotide have 100% complementarity.
Nucleic acid fragments encoding at least a portion of several floral development proteins have been isolated and identified by comparison of random plant cDNA sequences to public databases containing nucleotide and protein sequences using the BLAST algorithms well known to those skilled in the art.
The nucleic acid fragments of the instant invention may be used to isolate cDNAs and genes encoding homologous proteins from. the same or other plant species.
Isolation of homologous genes using sequence-dependent protocols is well known in the art. Examples of sequence-dependent protocols include, but are not limited to, methods of nucleic acid hybridization, and methods of DNA and RNA
amplification as exemplified by various uses of nucleic acid amplification technologies (e.g., polymerase chain reaction, ligase chain reaction).
For example, genes encoding other FT or Ap3 homolog, either as cDNAs or genomic DNAs, could be isolated directly by using all or a portion of the instant nucleic acid fragments as DNA hybridization probes to screen libraries from any desired plant employing methodology well known to those skilled in the art.
Specific oligonucleotide probes based upon the instant nucleic acid sequences can be designed and synthesized by methods known in the art (Maniatis). Moreover, an entire sequence can be used directly to synthesize DNA probes by methods known to the skilled artisan such as random primer DNA labeling, nick translation, end-labeling techniques, or RNA probes using available in vitro transcription systems. In addition, specific primers can be designed and used to amplify a part or all of the instant sequences. The resulting amplification products can be labeled directly during amplification reactions or labeled after amplification reactions, and used as probes to isolate full length cDNA or genomic fragments under conditions of appropriate stringency.
In addition, two short segments of the instant nucleic acid fragments may be used in polymerase chain reaction protocols to amplify longer nucleic acid fragments encoding homologous genes from DNA or RNA. The polymerase chain reaction may also be performed on a library of cloned nucleic acid fragments wherein the sequence of one primer is derived from the instant nucleic acid fragments, and the sequence of the other primer takes advantage of the presence of the polyadenylic acid tracts to the 3' end of the mRNA precursor encoding plant genes. Alternatively, the second primer sequence may be based upon sequences derived from the cloning vector. For example, the skilled artisan can follow the RACE protocol (Frohman et al. (1988) Proc. Natl. Acad. Sci. USA 85:8998-9002) to generate cDNAs by using PCR to amplify copies of the region between a single point in the transcript and the 3' or 5' end. Primers oriented in the 3' and 5' directions can be designed from the instant sequences. Using commercially available 3' RACE or 5' RACE systems (BRL), specific 3' or 5' cDNA fragments can be isolated (Ohara et al. (1989) Proc. NatL Acad. Sci. USA 86:5673-5677; Loh et al.
(1989) Science 243:217-220). Products generated by the 3' and 5' RACE
procedures can be combined to generate full-length cDNAs (Frohman and Martin (1989) Techniques 1:165). Consequently, a polynucleotide comprising a nucleotide sequence of at least 30 (preferably at least 40, most preferably at least 60) contiguous nucleotides derived from a nucleotide sequence selected from the group consisting of SEQ I D N0:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 37, 39, 41, 43, 45, 47, 49, 53, 55, 57, or 59 and the complement of such nucleotide sequences may be used in such methods to obtain a nucleic acid fragment encoding a substantial portion of an amino acid sequence of a polypeptide.
Availability of the instant nucleotide and deduced amino acid sequences facilitates immunological screening of cDNA expression libraries. Synthetic peptides representing portions of the instant amino acid sequences may be synthesized. These peptides can be used to immunize animals to produce polyclonal or monoclonal antibodies with specificity for peptides or proteins comprising the amino acid sequences. These antibodies can be then be used to screen cDNA expression libraries to isolate full-length cDNA clones of interest (Lerner (1984) Adv. Immunol. 36:1-34; Maniatis).

In another embodiment, this invention concerns viruses and host cells comprising either the chimeric genes of the invention as described herein or an isolated polynucleotide of the invention as described herein. Examples of host cells which can be used to practice the invention include, but are not limited to, yeast, bacteria, and plants.
As was noted above, the nucleic acid fragments of the instant invention may be used to create transgenic plants in which the disclosed polypeptides are present at higher or lower levels than normal or in cell types or developmental stages in which they are not normally found. This would have the effect of altering floral development and/or axillary meristems in transgenic plants. For instance, FT
is an activator of flowering, while FT-like proteins (TFL) are repressor of flowering.
Inhibition of TFL, or over-expression of FT, by chemical treatment, co-suppression, or mutation leads to a proliferation of flower formation which is useful for seed yield in crops such as corn, soybean, rice, and wheat. Over-expression of TFL, or inhibition of FT, suppresses flower formation which is useful for crops such as spinach or lettuce where leaves are desired and seed formation is not. The use of conditional promoters to control FT or TFL expression allows one to control the timing of flower formation, to delay flowering when vegetative growth is advantageous, or accelerate flowering in breeding where reduced generation time is desired. AP3 is required for the determination of the second and third whorls of the floral meristem which give rise to the petals and stamen. Suppression of AP3 has the effect of creating male-sterile flowers, which is advantageous in crops such as corn where outcrossing can lead to hybrid vigor. Induction of male-sterility in self-pollinating plants such as tomato has great commercial value in terms of breeding.
Overexpression of the proteins of the instant invention may be accomplished by first constructing a chimeric gene in which the coding region is operably linked to a promoter capable of directing expression of a gene in the desired tissues at the desired stage of development. The chimeric gene may comprise promoter sequences and translation leader sequences derived from the same genes. 3' Non-coding sequences encoding transcription termination signals may also be provided.
The instant chimeric gene may also comprise one or more introns in order to facilitate gene expression.
Plasmid vectors comprising the instant isolated polynucleotide (or chimeric gene) may be constructed. The choice of plasmid vector is dependent upon the method that will be used to transform host plants. The skilled artisan is well aware of the genetic elements that must be present on the plasmid vector in order to successfully transform, select and propagate host cells containing the chimeric gene. The skilled artisan will also recognize that different independent transformation events will result in different levels and patterns of expression (Jones et al. (1985) EM80 J. 4:2411-2418; De Almeida et al. (1989) Mol. Gen. Genetics 218:78-86), and thus that multiple events must be screened in order to obtain lines displaying the desired expression level and pattern. Such screening may be accomplished by Southern analysis of DNA, Northern analysis of mRNA
expression, Western analysis of protein expression, or phenotypic analysis.
For some applications it may be useful to direct the instant polypeptides to different cellular compartments, or to facilitate its secretion from the cell.
It is thus envisioned that the chimeric gene described above may be further supplemented by directing the coding sequence to encode the instant polypeptides with appropriate intracellular targeting sequences such as transit sequences (Keegstra (1989) Cell 56:247-253), signal sequences or sequences encoding endoplasmic reticulum localization (Chrispeels (1991 ) Ann. Rev. Plant Phys. Plant Mol. Biol. 42:21-53), or nuclear localization signals (Raikhel (1992) Plant Phys.100:1627-1632) with or without removing targeting sequences that are already present. While the references cited give examples of each of these, the list is not exhaustive and more targeting signals of use may be discovered in the future.
It may also be desirable to reduce or eliminate expression of genes encoding the instant polypeptides in plants for some applications. In order to accomplish this, a chimeric gene designed for co-suppression of the instant polypeptide can be constructed by linking a gene or gene fragment encoding that polypeptide to plant promoter sequences. Alternatively, a chimeric gene designed to express antisense RNA for all or part of the instant nucleic acid fragment can be constructed by linking the gene or gene fragment in reverse orientation to plant promoter sequences.
Either the co-suppression or antisense chimeric genes could be introduced into plants via transformation wherein expression of the corresponding endogenous genes are reduced or eliminated.
Molecular genetic solutions to the generation of plants with altered gene expression have a decided advantage over more traditional plant breeding approaches. Changes in plant phenotypes can be produced by specifically inhibiting expression of one or more genes by antisense inhibition or cosuppression (U.S. Patent Nos. 5,190,931, 5,107,065 and 5,283,323). An antisense or cosuppression construct would act as a dominant negative regulator of gene activity. While conventional mutations can yield negative regulation of gene activity these effects are most likely recessive. The dominant negative regulation available with a transgenic approach may be advantageous from a breeding perspective. In addition, the ability to restrict the expression of a specific phenotype to the reproductive tissues of the plant by the use of tissue specific promoters may confer agronomic advantages relative to conventional mutations which may have an effect in all tissues in which a mutant gene is ordinarily expressed.
The person skilled in the art will know that special considerations are associated with the use of antisense or cosuppression technologies in order to reduce expression of particular genes. For example, the proper level of expression of sense or antisense genes may require the use of different chimeric genes utilizing different regulatory elements known to the skilled artisan. Once transgenic plants are obtained by one of the methods described above, it will be necessary to screen individual transgenics for those that most effectively display the desired phenotype. Accordingly, the skilled artisan will develop methods for screening large numbers of transformants. The nature of these screens will generally be chosen on practical grounds. For example, one can screen by looking for changes in gene expression by using antibodies specific for the protein encoded by the gene being suppressed, or one could establish assays that specifically measure enzyme activity. A preferred method will be one which allows large numbers of samples to be processed rapidly, since it will be expected that a large number of transformants will be negative for the desired phenotype.
In another embodiment, the present invention relates to an isolated polypeptide comprising: (a) a first amino acid sequence comprising at least 50 or 100 amino acids, wherein the first amino acid sequence and the amino acid sequence of SEQ ID N0:6, SEQ ID NO:10, SEQ ID N0:12, SEQ ID N0:14, SEQ ID
N0:26, SEQ ID N0:28, SEQ ID N0:30, SEQ ID N0:34, or SEQ ID NO:42 have at least 80%, 85%, 90%, or 95% identity based on the Clustal alignment method, (b) a second amino acid sequence comprising at least 50 or 100 amino acids, wherein the second amino acid sequence and the amino acid sequence of SEQ ID N0:2 have at least 85%, 90%, or 95% identity based on the Clustal alignment method, (c) a third amino acid sequence comprising at least 100 amino acids, wherein the third amino acid sequence and the amino acid sequence of SEQ ID N0:4, SEQ ID
N0:16, or SEQ ID N0:40 have at least 80%, 85%, 90%, or 95% identity based on the Clustal alignment method, (d) a fourth amino acid sequence comprising at least 100 amino acids, wherein the fourth amino acid sequence and the amino acid sequence of SEQ ID N0:24 have at least 85%, 90%, or 95% identity based on the Clustal alignment method, (e) a fifth amino acid sequence comprising at least 150 amino acids, wherein the fifth amino acid sequence and the amino acid sequence of SEQ ID NO:8 or SEQ ID N0:44 have at least 80%, 85%, 90%, or 95%

identity based on the Clustal alignment method, (f) a sixth amino acid sequence comprising at least 150 amino acids, wherein the sixth amino acid sequence and the amino acid sequence of SEQ ID N0:38 have at least 85%, 90%, or 95% identity based on the Clustal alignment method, (g) a seventh amino acid sequence comprising at least 150 amino acids, wherein the seventh amino acid sequence and the amino acid sequence of SEQ ID N0:50 have at least 90% or 95% identity based on the Clustal alignment method, (h) an eighth amino acid sequence comprising at least 160 amino acids, wherein the eighth amino acid sequence and the amino acid sequence of SEQ ID N0:22 or SEQ ID N0:32 have at least 85%, 90%, or 95%
identity based on the Clustal alignment method, (i) a ninth amino acid sequence comprising at least 170 amino acids, wherein the ninth amino acid sequence and the amino acid sequence of SEQ ID N0:20 have at least 95% identity based on the Clustal alignment method, (j) a tenth amino acid sequence comprising at least 175 amino acids, wherein the tenth amino acid sequence and the amino acid sequence of SEQ ID N0:18 have at least 80%, 85%, 90%, or 95% identity based on the Clustal alignment method, or (k) an eleventh amino acid sequence comprising at least 200 amino acids, wherein the eleventh amino acid sequence and the amino acid sequence of SEQ ID N0:46 or SEQ ID N0:48 have at least 95% identity based on the Clustal alignment method. The first amino acid sequence preferably comprises the amino acid sequence of SEQ ID N0:6, SEQ ID N0:10, SEQ ID
N0:12, SEQ ID N0:14, SEQ ID N0:26, SEQ ID N0:28, SEQ ID N0:30, SEQ ID
N0:34, or SEQ ID NO:42, the second amino acid sequence preferably comprises the amino acid sequence of SEQ ID NO:2, the third amino acid sequence preferably comprises the amino acid sequence of SEQ ID N0:4, SEQ ID N0:16, or SEQ ID
N0:40, the fourth amino acid sequence preferably comprises the amino acid sequence of SEQ ID N0:24, the fifth amino acid sequence preferably comprises the amino acid sequence of SEQ ID NO:8 or SEQ ID N0:44, the sixth amino acid sequence preferably comprises the amino acid sequence of SEQ ID N0:38, the seventh amino acid sequence preferably comprises the amino acid sequence of SEQ ID N0:50, the eighth amino acid sequence preferably comprises the amino acid sequence of SEQ ID N0:22 or SEQ ID N0:32, the ninth amino acid sequence preferably comprises the amino acid sequence of SEQ ID N0:20, the tenth amino acid sequence preferably comprises the amino acid sequence of SEQ ID N0:18, and the eleventh amino acid sequence preferably comprises the amino acid sequence of SEQ ID N0:46 or SEQ ID N0:48. The polypeptide preferably is a FT
or Ap3 homolog.

The instant polypeptides (or portions thereof) may be produced in heterologous host cells, particularly in the cells of microbial hosts, and can be used to prepare antibodies to these proteins by methods well known to those skilled in the art. The antibodies are useful for detecting the polypeptides of the instant invention in situ in cells or in vitro in cell extracts. Preferred heterologous host cells for production of the instant polypeptides are microbial hosts. Microbial expression systems and expression vectors containing regulatory sequences that direct high level expression of foreign proteins are well known to those skilled in the art. Any of these could be used to construct a chimeric gene for production of the instant polypeptides. This chimeric gene could then be introduced into appropriate microorganisms via transformation to provide high level expression of the encoded floral development protein. An example of a vector for high level expression of the instant polypeptides in a bacterial host is provided (Example 7).
All or a substantial portion of the polynucleotides of the instant invention may also be used as probes for genetically and physically mapping the genes that they are a part of, and used as markers for traits linked to those genes. Such information may be useful in plant breeding in order to develop lines with desired phenotypes. For example, the instant nucleic acid fragments may be used as restriction fragment length polymorphism (RFLP) markers. Southern blots (Maniatis) of restriction-digested plant genomic DNA may be probed with the nucleic acid fragments of the instant invention. The resulting banding patterns may then be subjected to genetic analyses using computer programs such as MapMaker (Lander et al. (1987) Genomics 7:174-181) in order to construct a genetic map. In addition, the nucleic acid fragments of the instant invention may be used to probe Southern blots containing restriction endonuclease-treated genomic DNAs of a set of individuals representing parent and progeny of a defined genetic cross.
Segregation of the DNA polymorphisms is noted and used to calculate the position of the instant nucleic acid sequence in the genetic map previously obtained using this population (Botstein et al. (1980) Am. J. Hum. Genet. 32:314-331).
The production and use of plant gene-derived probes for use in genetic mapping is described in Bernatzky and Tanksley (1986) Plant Mol. BioL Reporter 4:37-41. Numerous publications describe genetic mapping of specific cDNA
clones using the methodology outlined above or variations thereof. For example, F2 intercross populations, backcross populations, randomly mated populations, near isogenic lines, and other sets of individuals may be used for mapping. Such methodologies are well known to those skilled in the art.

Nucleic acid probes derived from the instant nucleic acid sequences may also be used for physical mapping (i.e., placement of sequences on physical maps;
see Hoheisel et al. In: Nonmammalian Genomic Analysis: A Practical Guide, Academic press 1996, pp. 319-346, and references cited therein).
Nucleic acid probes derived from the instant nucleic acid sequences may be used in direct fluorescence in situ hybridization (FISH) mapping (Trask (1991) Trends Genet. 7:149-154). Although current methods of FISH mapping favor use of large clones (several to several hundred KB; see Laan et al. (1995) Genome Res.
5:13-20), improvements in sensitivity may allow performance of FISH mapping using shorter probes.
A variety of nucleic acid amplification-based methods of genetic and physical mapping may be carried out using the instant nucleic acid sequences. Examples include allele-specific amplification (Kazazian (1989) J. Lab. Clin. Med.
11:95-96), polymorphism of PCR-amplified fragments (CAPS; Sheffield et al. (1993) Genomics 16:325-332), allele-specific ligation (Landegren et al. (1988) Science 241:1077-1080), nucleotide extension reactions (Sokolov (1990) Nucleic Acid Res.
18:3671), Radiation Hybrid Mapping (Walter et al. (1997) Nat. Genet. 7:22-28) and Happy Mapping (Dear and Cook (1989) Nucleic Acid Res. 17:6795-6807). For these methods, the sequence of a nucleic acid fragment is used to design and produce primer pairs for use in the amplification reaction or in primer extension reactions. The design of such primers is well known to those skilled in the art. In methods employing PCR-based genetic mapping, it may be necessary to identify DNA sequence differences between the parents of the mapping cross in the region corresponding to the instant nucleic acid sequence. This, however, is generally not necessary for mapping methods.
Loss of function mutant phenotypes may be identified for the instant cDNA
clones either by targeted gene disruption protocols or by identifying specific mutants for these genes contained in a maize population carrying mutations in all possible genes (Ballinger and Benzer (1989) Proc. Natl. Acad. Sci USA 86:9402-9406;
Koes et al. (1995) Proc. Natl. Acad. Sci USA 92:8149-8153; Bensen et al. (1995) Plant Cell 7:75-84). The latter approach may be accomplished in two ways. First, short segments of the instant nucleic acid fragments may be used in polymerase chain reaction protocols in conjunction with a mutation tag sequence primer on DNAs prepared from a population of plants in which Mutator transposons or some other mutation-causing DNA element has been introduced (see Bensen, supra). The amplification of a specific DNA fragment with these primers indicates the insertion of the mutation tag element in or near the plant gene encoding the instant polypeptides. Alternatively, the instant nucleic acid fragment may be used as a hybridization probe against PCR amplification products generated from the mutation population using the mutation tag sequence primer in conjunction with an arbitrary genomic site primer, such as that for a restriction enzyme site-anchored synthetic adaptor. With either method, a plant containing a mutation in the endogenous gene encoding the instant polypeptides can be identified and obtained. This mutant plant can then be used to determine or confirm the natural function of the instant polypeptides disclosed herein.
EXAMPLES
The present invention is further defined in the following Examples, in which parts and percentages are by weight and degrees are Celsius, unless otherwise stated. It should be understood that these Examples, while indicating preferred embodiments of the invention, are given by way of illustration only. From the above discussion and these Examples, one skilled in the art can ascertain the essential characteristics of this invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions. Thus, various modifications of the invention in addition to those shown and described herein will be apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims.
The disclosure of each reference set forth herein is incorporated herein by reference in its entirety.

Composition of cDNA Libraries; Isolation and Sequencing of cDNA Clones cDNA libraries representing mRNAs from various Peruvian lily (Alstroemeria caryophylla), balsam pear (Momordica charantia), garden balsam (Impatiens balsamic), corn (Zea mays), rice (Oryza sativa), soybean (Glycine max), and wheat (Triticum aestivum) tissues were prepared. The characteristics of the libraries are described below. Corn developmental stages are explained in the publication "How a corn plant develops" from the Iowa State University Coop. Ext. Service Special Report No. 48 reprinted June 1993.

r.

cDNA Libraries from Peruvian Lily, Balsam Pear, Garden Balsam, Corn, Rice, Soybean, and Wheat Library Tissue Clone cbn10 Corn Developing Kernel (Embryo and Endosperm); cbn10.pk0052.f5 10 Days After Pollination cbn2 Corn Developing Kernel Two Days After cbn2.pk0035.f12 Pollination cc71 Corn Callus Type I I Tissue, Somatic cc71 se-se-b Embryo Formed b.pk0003.h10 cco1n Corn Cob of 67 Day Old Plants Grown in cco1n.pk0010.h3 Green House* cco1 n.pk0037.d cen3n Corn Endosperm 20 Days After Pollination*cen3n.pk0004.e9 cen3n.pk0047.h7 cen3n.pk0093.f1 cen3n.pk0165.f1 cta1 Corn Tassel* cta1 n.pk0050.f8 n cta1 n.pk0058.d11 b ctn1c Corn Tassel, Night Harvested ctnlc.pk002.j23 eall Peruvian Lily Mature Leaf from Mature eall c.pk006.e6 c Stem fds Balsam Pear Developing Seed fds.pk0003.h2 fds.pk0026.d10 fds1 Balsam Pear Developing Seed fds1 n.pk001.p18 n ids Garden Balsam Developing Seed ids.pk0031.a5 p0014 Corn Leaf p0014.ctush42r p0081 Corn Pedicel 10 Days After Pollination p0081.chcad07r p0095 Ear Leaf Sheath*; Growth Conditions: Field; Control p0095.cwsas14f or Untreated Tissues; Growth Stage: 2-3 weeks After Pollen Shed p0104 Corn V5-Stage Root Infested With Corn Root Worm* p0104.cabak14rb p0118 Corn Stem Tissue Pooled From the 4-5 Internodes p0118.chsaq04rb Subtending The Tassel At Stages V8-V12, Night Harvested*
p0119 Corn V12-Stage Ear Shoot With Husk, Night p0119.cmtmg45rb Harvested*
p0120 Pooled Endosperm: 18, 21, 24, 27 and 29 Days After p0120.cdeae63r Pollination*
p0128 Corn Primary and Secondary Immature Ear p0128.cpicl42r Library Tissue Clone rbm1c Rice Bran 0 Hrs After Milling rbm1c:pk001.a6 rl0n Rice 15 Day Old Leaf* rl0n.pk0022.h10 rl0n.pk0022.h11 r1r48 Resistant Rice Leaf 15 Days After Germination, 48 rIr48.pk0001.b1 Hours After Infection of Strain Magnaporthe grisea 4360-R-62 (AVR2-YAMO) r1s24 Susceptible Rice Leaf 15 Days After Germination, 24 rIs24.pk0017.c7 Hours After Infection of Strain Magnaporthe grisea 4360-R-67 (AVR2-YAMO) rr1 Rice Root of Two Week Old Developing Seedling rr1.pk0043.f9 rsr9n Rice Leaf 15 Days After Germination, Harvested 2-72 rsr9n.pk001.d1 Hours Following Infection With Magnaporthe grisea (4360-R-62 and 4360-R-67)*

sea Soybean Embryo, 17 Days After Flowering se3.pk0036.g4 se6 Soybean Embryo, 26 Days After Flowering se6.pk0039.h6 sfl1 Soybean Immature Flower* sfl1 n.pk001.l16 n srr2c Soybean 8-Day-Old Root srr2c.pk002.o7 ssl Soybean Seedling 5-10 Days After GerminationssLpk0007.a9 wdk2c Wheat Developing Kernel, 7 Days After wdk2c.pk012.o17 Anthesis wdk2c.pk017.p21 wdk2c.pk008.n3 wdk9n1 Wheat Kernels 3, 7, 14 and 21 Days After Anthesis* wdk9n1.pk001.020 *These libraries were normalized essentially as described in U.S. Patent No. 5,482,845, incorporated herein by reference.
cDNA libraries may be prepared by any one of many methods available. For example, the cDNAs may be introduced into plasmid vectors by first preparing the cDNA libraries in Uni-ZAPTM XR vectors according to the manufacturer's protocol (Stratagene Cloning Systems, La Jolla, CA). The Uni-ZAPTM XR libraries are converted into plasmid libraries according to the protocol provided by Stratagene.
Upon conversion, cDNA inserts will be contained in the plasmid vector pBluescript.
In addition, the cDNAs may be introduced directly into precut Bluescript II
SK(+) vectors (Stratagene) using T4 DNA ligase (New England Biolabs), followed by transfection into DH10B cells according to the manufacturer's protocol (GIBCO
BRL
Products). Once the cDNA inserts are in plasmid vectors, plasmid DNAs are prepared from randomly picked bacterial colonies containing recombinant pBluescript plasmids, or the insert cDNA sequences are amplified via polymerase chain reaction using primers specific for vector sequences flanking the inserted cDNA sequences. Amplified insert DNAs or plasmid DNAs are sequenced in dye-primer sequencing reactions to generate partial cDNA sequences (expressed sequence tags or "ESTs"; see Adams et al., (1991) Science 252:1651-1656). The resulting ESTs are analyzed using a Perkin Elmer Model 377 fluorescent sequencer.
Full-insert sequence (FIS) data is generated utilizing a modified transposition protocol. Clones identified for FIS are recovered from archived glycerol stocks as single colonies, and plasmid DNAs are isolated via alkaline lysis. Isolated DNA
templates are reacted with vector primed M13 forward and reverse oligonucleotides in a PCR-based sequencing reaction and loaded onto automated sequencers.
Confirmation of clone identification is performed by sequence alignment to the original EST sequence from which the FIS request is made.
Confirmed templates are transposed via the Primer Island transposition kit (PE Applied Biosystems, Foster City, CA) which is based upon the Saccharomyces cerevisiae Ty1 transposable element (Devine and Boeke (1994) Nucleic Acids Res.
22:3765-3772). The in vitro transposition system places unique binding sites randomly throughout a population of large DNA molecules. The transposed DNA is then used to transform DH10B electro-competent cells (Gibco BRL/Life Technologies, Rockville, MD) via electroporation. The transposable element contains an additional selectable marker (named DHFR; Fling and Richards (1983) Nucleic Acids Res. 77:5147-5158), allowing for dual selection on agar plates of only those subclones containing the integrated transposon. Multiple subclones are randomly selected from each transposition reaction, plasmid DNAs are prepared via alkaline lysis, and templates are sequenced (ABI Prism dye-terminator ReadyReaction mix) outward from the transposition event site, utilizing unique primers specific to the binding sites within the transposon.
Sequence data is collected (ABI Prism Collections) and assembled using Phred/Phrap (P. Green, University of Washington, Seattle). Phred/Phrap is a public domain software program which re-reads the ABI sequence data, re-calls the bases, assigns quality values, and writes the base calls and quality values into editable output files. The Phrap sequence assembly program uses these quality values to increase the accuracy of the assembled sequence contigs. Assemblies are viewed by the Consed sequence editor (D. Gordon, University of Washington, Seattle).
In some of the clones the cDNA fragment corresponds to a portion of the 3'-terminus of the gene and does not cover the entire open reading frame. In order to obtain the upstream information one of two different protocols are used.
The first of these methods results in the production of a fragment of DNA containing a portion of the desired gene sequence while the second method results in the production of a fragment containing the entire open reading frame. Both of these methods use two rounds of PCR amplification to obtain fragments from one or more libraries.
The libraries some times are chosen based on previous knowledge that the specific gene should be found in a certain tissue and some times are randomly-chosen.
Reactions to obtain the same gene may be performed on several libraries in parallel or on a pool of libraries. Library pools are normally prepared using from 3 to different libraries and normalized to a uniform dilution. In the first round of amplification both methods use a vector-specific (forward) primer corresponding to a portion of the vector located at the 5'-terminus of the clone coupled with a gene-specific (reverse) primer. The first method uses a sequence that is complementary to a portion of the already known gene sequence while the second method uses a gene-specific primer complementary to a portion of the 3'-untranslated region (also referred to as UTR). In the second round of amplification a nested set of primers is used for both methods. The resulting DNA
fragment is ligated into a pBluescript vector using a commercial kit and following the manufacturer's protocol. This kit is selected from many available from several vendors including Invitrogen (Carlsbad, CA), Promega Biotech (Madison, WI), and Gibco-BRL (Gaithersburg, MD). The plasmid DNA is isolated by alkaline lysis method and submitted for sequencing and assembly using Phred/Phrap, as above.

Identification of cDNA Clones cDNA clones encoding floral development proteins were identified by conducting BLAST (Basic Local Alignment Search Tool; Altschul et al. (1993) J. Mol. Biol. 275:403-410; see also the explanation of the BLAST alogarithm on the world wide web site for the National Center for Biotechnology Information at the National Library of Medicine of the National Institutes of Health) searches for similarity to sequences contained in the BLAST "nr" database (comprising all non-redundant GenBank CDS translations, sequences derived from the 3-dimensional structure Brookhaven Protein Data Bank, the last major release of the SWISS-PROT protein sequence database, EMBL, and DDBJ databases). The cDNA sequences obtained in Example 1 were analyzed for similarity to all publicly available DNA sequences contained in the.,"nr" database using the BLASTN
algorithm provided by the National Center for Biotechnology Information (NCBI).
The DNA sequences were translated in all reading frames and compared for similarity to all publicly available protein sequences contained in the "nr"
database using the BLASTX algorithm (Gish and States (1993) Nat. Genet. 3:266-272) provided by the NCBI. For convenience, the P-value (probability) of observing a match of a cDNA sequence to a sequence contained in the searched databases merely by chance as calculated by BLAST are reported herein as "pLog" values, which represent the negative of the logarithm of the reported P-value.
Accordingly, the greater the pLog value, the greater the likelihood that the cDNA sequence and the BLAST "hit" represent homologous proteins.
ESTs submitted for analysis are compared to the genbank database as described above. ESTs that contain sequences more 5- or 3-prime can be found by using the BLASTn algorithm (Altschul et al (1997) Nucleic Acids Res.
25:3389-3402.) against the DuPont proprietary database comparing nucleotide sequences that share common or overlapping regions of sequence homology.
Where common or overlapping sequences exist between two or more nucleic acid fragments, the sequences can be assembled into a single contiguous nucleotide sequence, thus extending the original fragment in either the 5 or 3 prime direction.
Once the most 5-prime EST is identified, its complete sequence can be determined by Full Insert Sequencing as described in Example 1. Homologous genes belonging to different species can be found by comparing the amino acid sequence of a known gene (from either a proprietary source or a public database) against an EST database using the tBLASTn algorithm. The tBLASTn algorithm searches an amino acid query against a nucleotide database that is translated in all 6 reading frames. This search allows for differences in nucleotide codon usage between different species, and for codon degeneracy.

Characterization of cDNA Clones Encoding Flowering Locus T (FT Homologs The BLASTX search using the EST sequences from clones listed in Table 3 revealed similarity of the polypeptides encoded by the cDNAs to FT and its homologs from Citrus unshiu (NCBI GenBank Identifier (GI) No. 4903139), Arabidopsis thaliana (NCBI GI Nos. 5002246 and 2190540), Oryza sativa (NCBI GI
Nos. 5360178 and 5360180) and Nicotiana tabacum (NCBI GI No. 5453314).
Shown in Table 3 are the BLAST results for individual ESTs ("EST"), the sequences of the entire cDNA inserts comprising the indicated cDNA clones ("FIS"), the sequences of contigs assembled from two or more ESTs ("Contig"), sequences of contigs assembled from an FIS and one or more ESTs ("Contig*"), or sequences encoding an entire protein derived from an FIS, a contig, or an FIS and PCR
fragment sequence ("CGS"):

BLAST Results for Sequences Encoding Polypeptides Homologous to Flowering Locus T (FT) Protein BLAST Results Clone Status NCBI Gl No. Lo Score eal1 c.pk006.e6 EST 4903139 65.70 Contig of CGS 5002246 75.00 fds.pk0003.h2 fds.pk0026.d10 fdsl n.pk001.p18 ids.pk0031.a5 (FIS) CGS 5002246 61.70 Contig of CGS 4903139 77.00 cbn10.pk0052.f5 cbn2.pk0035.f12 ccol n.pk0010.h3 p0095.cwsas14f p0119.cmtmg45rb p0128.cpicl42r cc71se-b.pk0003.h10 (FIS)CGS 5002246 59.15 cco1n.pk0037.d10 (FIS) CGS 2190540 68.52 Contig of CGS 5002246 57.30 cen3n.pk0004.e9 cen3n.pk0047.h7 cen3n.pk0093.f1 cen3n.pk0165.f1 p0120.cdeae63r p0014.ctush42r (FIS) CGS 4903139 59.00 p0081.chcad07r (FIS) CGS 4903139 81.00 p0104.cabak14rb (FIS) CGS 5360178 93.70 p0118.chsaq04rb (FIS) CGS 5360180 82.10 rbm1 c.pk001.a6 EST 5002246 46.04 Contig of Contig 4903139 43.30 rl0n. pk0022. h 10 rl0n.pk0022.h11 rIr48.pk0001.b1 EST 5002246 14.52 rIs24.pk0017.c7 (FIS) CGS 5002246 64.70 rr1.pk0043.f9 (FIS) CGS 5360178 82.10 rsr9n.pk001.d1 EST 2190540 35.70 BLAST Results Clone Status NCBI GI No. Lo Score Contig of CGS 5002246 76.00 se3.pk0036.g4 se6.pk0039.h6 (FIS) srr2c.pk002.o7 (FIS) CGS 5360180 77.52 Contig of CGS 5453314 73.70 ssl.pk0007.a9 PCR fragment sequence wdk2c.pk012.o17 (FIS) CGS 5002246 62.30 wdk9n1.pk001.o20 (FIS) CGS 4903139 75.70 cta1 n.pk0058.d 11 b FIS 15218709 53.40 rbm1 c.pk001.a6:fis CGS 5002246 56.10 rl0n.pk0022.h 1 O:fis FIS 14517620 36.70 rsr9n.pk001.d1:fis CGS 15218709 70.39 The PCR fragment that was used to extend the nucleotide sequence obtained from clone ssLpk0007.a9 was obtained via methods (e.g., RACE
techniques) well-known to those skilled in the art.
The amino acid sequence of the polypeptide encoded by the insert in clone wdk2c.pk012.o17 is identical to the amino acid sequence of the polypeptide encoded by the nucleotide sequence of a contig assembled from ESTs derived from clones wdk2c.pk008.n3, wdk2c.pk012.o17, and wdk2c.pk017.p21.
Figure 1 presents an alignment of the amino acid sequences set forth in SEQ
ID NOs:4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 30, 32, 36, 38, 40, 42, and 44 and the Oryza sativa sequence (NCBI GI No. 5360178; SEQ ID N0:51).
The data in Table 4 represents a calculation of the percent identity of the amino acid sequences set forth in SEQ ID NOs:4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 30, 32, 36, 38, 40, 42, and 44 and the Oryza sativa sequence (NCBI GI
No. 5360178; SEQ ID N0:51).

Percent Identity of Amino Acid Sequences Deduced From the Nucleotide Sequences of cDNA Clones Encoding Polypeptides Homologous to FT Protein Percent Identity to SEQ ID NO. NCBI GI No.

2 69.3 [gi 4903139]

4 72.3 [gi 5002246]

6 61.3 [gi 5002246]

8 74.6 [gi 4903139]

60.5 [gi 5002246]

12 67.2 [g i 2190540]

14 60.1 [gi 5002246]

16 59.9 [gi 4903139]

18 78.0 [gi 4903139]

94.8 [gi 5360178]

22 83.8 [gi 5360180]

24 56.2 [gi 5002246]

26 64.8 [gi 4903139]

28 52.9 [gi 5002246]

65.9 [gi 5002246]

32 83.2 [gi 5360178]

36 74.4 [gi 5002246]

38 76.3 [gi 5360180]

74.6 [gi 5453314]

42 64.2 [gi 5002246]

44 72.9 [gi 4903139]

54 60.8 [gi 15218709]

56 57.2 [gi 5002246]

58 66.3 [gi14517620]

60 69.0 [gi 15218709]

5 Sequence alignments and percent identity calculations were performed using the Megalign program of the LASERGENE bioinformatics computing suite (DNASTAR Inc., Madison, WI). Multiple alignment of the sequences was performed using the Clustal method of alignment (Higgins and Sharp (1989) CABIOS.
5:151-153) with the default parameters (GAP PENALTY=10, GAP LENGTH
10 PENALTY=10). Default parameters for pairwise alignments using the Clustal method were KTUPLE 1, GAP PENALTY=3, WINDOW=5 and DIAGONALS

SAVED=5. It will be recognized by one skilled in the art that conserved sequence elements within the encoded polypeptide are useful in identifying homologous enzymes. Two such elements, although not necessarily the only elements, are the sequences Asp-Pro-Asp-Xaa-Pro-Xaa-Pro-Ser-Xaa-Pro found, for example, at positions 70-79 of SEQ ID N0:4, and Gly-Ile-His-Arg found, for example at positions 115-118 of SEQ ID N0:4. Sequence alignments and BLAST scores and probabilities indicate that the nucleic acid fragments comprising the instant cDNA
clones encode a substantial portion of a polypeptide encoded by a member of TFL1/FT gene family. These sequences represent the first Peruvian lily, balsam pear, garden balsam, corn, soybean, wheat sequences and new rice sequences encoding flowering locus T (FT or TFL) homologs known to~Applicant.

Characterization of cDNA Clones Encoding Ap3 Homologs The BLASTX search using the EST sequences from clones listed in Table 5 revealed similarity of the polypeptides encoded by the cDNAs to MADS box proteins (Ap3 homologs) from Oryza sativa (NCBI GI Nos. 5295980 and 7446534) and Medicago sativa (NCBI GI No. 2827300). Shown in Table 5 are the BLAST results for individual ESTs ("EST"), the sequences of the entire cDNA inserts comprising the indicated cDNA clones ("FIS"), the sequences of contigs assembled from two or more ESTs ("Contig"), sequences of contigs assembled from an FIS and one or more ESTs ("Contig'~"), or sequences encoding an entire protein derived from an FIS, a contig, or an FIS and PCR ("CGS"):

BLAST Results for Sequences Encoding Polypeptides Homologous to Ap3 Protein BLAST Results Clone Status NCBI GI No. Lo Score ctal n.pk0050.f8 (FIS) CGS 5295980 113.00 ctn1c.pk002.j23 (FIS) CGS 7446534 109.00 sfl1 n.pk001.l16 (FIS) CGS 2827300 114.00 Figure 2 presents an alignment of the amino acid sequences set forth in SEQ
ID NOs:46, 48, and 50 and the Oryza sativa sequence (NCBI GI No. 5295980; SEQ
ID N0:52). The data in Table 6 represents a calculation of the percent identity of the amino acid sequences set forth in SEQ ID NOs:46, 48, and 50 and the Oryza sativa sequence (NCBI GI No. 5295980; SEQ ID N0:52).

Percent Identity of Amino Acid Sequences Deduced From the Nucleotide Sequences of cDNA Clones Encoding Polypeptides Homologous to Ap3 Protein Percent Identity to SEQ ID NO. NCBI GI No.
46 86.6 [gi 5295980]
48 91.4 [gi 7446534]
50 85.9 [gi 2827300]
Sequence alignments and percent identity calculations were performed using the Megalign program of the LASERGENE bioinformatics computing suite (DNASTAR Inc., Madison, WI). Multiple alignment of the sequences was performed using the Clustal method of alignment (Higgins and Sharp (1989) CABIOS.
5:151-153) with the default parameters (GAP PENALTY=10, GAP LENGTH
PENALTY=10). Default parameters for pairwise alignments using the Clustal method were KTUPLE 1, GAP PENALTY=3, WINDOW=5 and DIAGONALS
SAVED=5. It will be recognized by one skilled in the art that conserved sequence elements within the encoded polypeptide are useful in identifying homologous enzymes. One such element, although not necessarily the only element, is the sequence Arg-Gly-Lys-Ile-Xaa-Ile-Lys-Arg-Ile-Glu-Asn-Xaa-Thr-Asn-Arg-Gln-Val-Thr-Xaa-Ser-Lys-Arg-Arg-Xaa-Gly-Xaa-Xaa-Lys-Lys-Ala found, for example, at positions 3-32 of SEQ ID N0:46. Sequence alignments and BLAST scores and probabilities indicate that the nucleic acid fragments comprising the instant cDNA
clones encode a substantial portion of an Ap3 homolog. These sequences represent the first soybean sequence and new corn sequences encoding Ap3 homologs known to Applicant.

Expression of Chimeric Genes in Monocot Cells A chimeric gene comprising a cDNA encoding the instant polypeptides in sense orientation with respect to the maize 27 kD zein promoter that is located 5' to the cDNA fragment, and the 10 kD zein 3' end that is located 3' to the cDNA
fragment, can be constructed. The cDNA fragment of this gene may be generated by polymerase chain reaction (PCR) of the cDNA clone using appropriate oligonucleotide primers. Cloning sites (Ncol or Smal) can be incorporated into the oligonucleotides to provide proper orientation of the DNA fragment when inserted into the digested vector pML103 as described below. Amplification is then performed in a standard PCR. The amplified DNA is then digested with restriction enzymes Ncol and Smal and fractionated on an agarose gel. The appropriate band can be isolated from the gel and combined with a 4.9 kb Ncol-Smal fragment of the plasmid pML103. Plasmid pML103 has been deposited under the terms of the Budapest Treaty at ATCC (American Type Culture Collection, 10801 University Blvd., Manassas, VA 20110-2209), and bears accession number ATCC 97366. The DNA segment from pML103 contains a 1.05 kb Sall-Ncol promoter fragment of the maize 27 kD zein gene and a 0.96 kb Smal-Sall fragment from the 3' end of the maize 10 kD zein gene in the vector pGem9Zf(+) (Promega). Vector and insert DNA can be legated at 15°C overnight, essentially as described (Maniatis). The legated DNA may then be used to transform E. coli XL1-Blue (Epicurean Coli XL-BIueT""; Stratagene). Bacterial transformants can be screened by restriction enzyme digestion of plasmid DNA and limited nucleotide sequence analysis using the dideoxy chain termination method (SequenaseT"~ DNA Sequencing Kit; U.S.
Biochemical). The resulting plasmid construct would comprise a chimeric gene encoding, in the 5' to 3' direction, the maize 27 kD zein promoter, a cDNA
fragment IS encoding the instant polypeptides, and the 10 kD zein 3' region.
The chimeric gene described above can then be introduced into corn cells by the following procedure. Immature corn embryos can be dissected from developing caryopses derived from crosses of the inbred corn lines H99 and LH132. The embryos are isolated 10 to 11 days after pollination when they are 1.0 to 1.5 mm long. The embryos are then placed with the axis-side facing down and in contact with agarose-solidified N6 medium (Chu et al. (1975) Sci. Sin. Peking 18:659-668).
The embryos are kept in the dark at 27°C. Friable embryogenic callus consisting of undifferentiated masses of cells with somatic proembryoids and embryoids borne on suspensor structures proliferates from the scutellum of these immature embryos.
The embryogenic callus isolated from the primary explant can be cultured on N6 medium and sub-cultured on this medium every 2 to 3 weeks.
The plasmid, p35S/Ac (obtained from Dr. Peter Eckes, Hoechst Ag, Frankfurt, Germany) may be used in transformation experiments in order to provide for a selectable marker. This plasmid contains the Pat gene (see European Patent Publication 0 242 236) which encodes phosphinothricin acetyl transferase (PAT).
The enzyme PAT confers resistance to herbicidal glutamine synthetase inhibitors such as phosphinothricin. The pat gene in p35S/Ac is under the control of the promoter from Cauliflower Mosaic Virus (Odell et al. (1985) Nature 313:810-812) and the 3' region of the nopaline synthase gene from the T-DNA of the Ti plasmid of Agrobacterium tumefaciens.
The particle bombardment method (Klein et al. (1987) Nature 327:70-73) may be used to transfer genes to the callus culture cells. According to this method, gold particles (1 p.m in diameter) are coated with DNA using the following technique.
Ten p.g of.plasmid DNAs are added to 50 wL of a suspension of gold particles (60 mg per mL). Calcium chloride (50 ~,L of a 2.5 M solution) and spermidine free base (20 ~,L of a 1.0 M solution) are added to the particles. The suspension is vortexed during the addition of these solutions. After 10 minutes, the tubes are briefly centrifuged (5 sec at 15,000 rpm) and the supernatant removed. The particles are resuspended in 200 ~.L of absolute ethanol, centrifuged again and the supernatant removed. The ethanol rinse is performed again and the particles resuspended in a final volume of 30 p,L of ethanol. An aliquot (5 p,L) of the DNA-coated gold particles can be placed in the center of a KaptonT"" flying disc (Bio-Rad Labs). The particles are then accelerated into the corn tissue with a BiolisticT""
PDS-1000/He (Bio-Rad Instruments, Hercules CA), using a helium pressure of 1000 psi, a gap distance of 0.5 cm and a flying distance of 1.0 cm.
For bombardment, the embryogenic tissue is placed on filter paper over agarose-solidified N6 medium. The tissue is arranged as a thin lawn and covered a circular area of about 5 cm in diameter. The petri dish containing the tissue can be placed in the chamber of the PDS-1000/He approximately 8 cm from the stopping screen. The air in the chamber is then evacuated to a vacuum of 28 inches of Hg.
The macrocarrier is accelerated with a helium shock wave using a rupture membrane that bursts when the He pressure in the shock tube reaches 1000 psi.
Seven days after bombardment the tissue can be transferred to N6 medium that contains bialophos (5 mg per liter) and lacks casein or proline. The tissue continues to grow slowly on this medium. After an additional 2 weeks the tissue can be transferred to fresh N6 medium containing bialophos. After 6 weeks, areas of about 1 cm in diameter of actively growing callus can be identified on some of the plates containing the bialophos-supplemented medium. These calli may continue to grow when sub-cultured on the selective medium.
Plants can be regenerated from the transgenic callus by first transferring clusters of tissue to N6 medium supplemented with 0.2 mg per liter of 2,4-D.
After two weeks the tissue can be transferred to regeneration medium (Fromm et al.
(1990) BiolTechnology 8:833-839).

Expression of Chimeric Genes in Dicot Cells A seed-specific expression cassette composed of the promoter and transcription terminator from the gene encoding the ~i subunit of the seed storage protein phaseolin from the bean Phaseolus vulgaris (Doyle et al. (1986) J.
Biol.
Chem. 261:9228-9238) can be used for expression of the instant polypeptides in transformed soybean. The phaseolin cassette includes about 500 nucleotides upstream (5') from the translation initiation codon and about 1650 nucleotides downstream (3') from the translation stop codon of phaseolin. Between the 5' and 3' regions are the unique restriction endonuclease sites Ncol (which includes the ATG
translation initiation codon), Smal, Kpnl and Xbal. The entire cassette is flanked by Hindlll sites.
The cDNA fragment of this gene may be generated by polymerase chain reaction (PCR) of the cDNA clone using appropriate oligonucleotide primers.
Cloning sites can be incorporated into the oligonucleotides to provide proper orientation of the DNA fragment when inserted into the expression vector.
Amplification is then performed as described above, and the isolated fragment is inserted into a pUC18 vector carrying the seed expression cassette.
Soybean embryos may then be transformed with the expression vector comprising sequences encoding the instant polypeptides. To induce somatic embryos, cotyledons, 3-5 mm in length dissected from surface sterilized, immature seeds of the soybean cultivar A2872, can be cultured in the light or dark at 26°C on an appropriate agar medium for 6-10 weeks. Somatic embryos which produce secondary embryos are then excised and placed into a suitable liquid medium.
After repeated selection for clusters of somatic embryos which multiplied as early, globular staged embryos, the suspensions are maintained as described below.
Soybean embryogenic suspension cultures can be maintained in 35 mL liquid media on a rotary shaker, 150 rpm, at 26°C with florescent lights on a 16:8 hour day/night schedule. Cultures are subcultured every two weeks by inoculating approximately 35 mg of tissue into 35 mL of liquid medium.
Soybean embryogenic suspension cultures may then be transformed by the method of particle gun bombardment (Klein et al. (1987) Nature (London) 327:70-73, U.S. Patent No. 4,945,050). A DuPont BiolisticT"" PDS1000/HE
instrument (helium retrofit) can be used for these transformations.
A selectable marker gene which can be used to facilitate soybean transformation is a chimeric gene composed of the 35S promoter from Cauliflower Mosaic Virus (Odell et al. (1985) Nature 313:810-812), the hygromycin phosphotransferase gene from plasmid pJR225 (from E, coli; Gritz et al. (1983) Gene 25:179-188) and the 3' region of the nopaline synthase gene from the T-DNA
of the Ti plasmid of Agrobacterium tumefaciens. The seed expression cassette comprising the phaseolin 5' region, the fragment encoding the instant polypeptides and the phaseolin 3' region can be isolated as a restriction fragment. This fragment can then be inserted into a unique restriction site of the vector carrying the marker gene.
To 50 ~,L of a 60 mg/mL 1 ~,m gold particle suspension is added (in order):
~,L DNA (1 ~g/~,L), 20 ~,L spermidine (0.1 M), and 50 g,L CaCl2 (2.5 M). The 5 particle preparation is then agitated for three minutes, spun in a microfuge for seconds and the supernatant removed. The DNA-coated particles are then washed once in 400 ~,L 70% ethanol and resuspended in 40 ~,L of anhydrous ethanol. The DNA/particle suspension can be sonicated three times for one second each. Five pL of the DNA-coated gold particles are then loaded on each macro 10 carrier disk.
Approximately 300-400 mg of a two-week-old suspension culture is placed in an empty 60x15 mm petri dish and the residual liquid removed from the tissue with a pipette. For each transformation experiment, approximately 5-10 plates of tissue are normally bombarded. Membrane rupture pressure is set at 1100 psi and the chamber is evacuated to a vacuum of 28 inches mercury. The tissue is placed approximately 3.5 inches away from the retaining screen and bombarded three times. Following bombardment, the tissue can be divided in half and placed back into liquid and cultured as described above.
Five to seven days post bombardment, the liquid media may be exchanged with fresh media, and eleven to twelve days post bombardment with fresh media containing 50 mg/mL hygromycin. This selective media can be refreshed weekly.
Seven to eight weeks post bombardment, green, transformed tissue may be observed growing from untransformed, necrotic embryogenic clusters. Isolated green tissue is removed and inoculated into individual flasks to generate new, clonally propagated, transformed embryogenic suspension cultures. Each new line may be treated as an independent transformation event. These suspensions can then be subcultured and maintained as clusters of immature embryos or regenerated into whole plants by maturation and germination of individual somatic embryos.

Expression of Chimeric Genes in Microbial Cells The cDNAs encoding the instant polypeptides can be inserted into the T7 E, coli expression vector pBT430. This vector is a derivative of pET-3a (Rosenberg et al. (1987) Gene 56:125-135) which employs the bacteriophage T7 RNA
polymerase/T7 promoter system. Plasmid pBT430 was constructed by first destroying the EcoRl and Hindlll sites in pET-3a at their original positions.
An oligonucleotide adaptor containing EcoRl and Hind III sites was inserted at the BamHl site of pET-3a. This created pET-3aM with additional unique cloning sites for insertion of genes into the expression vector. Then, the Ndel site at the position of translation initiation was converted to an Ncol site using oligonucleotide-directed mutagenesis. The DNA sequence of pET-3aM in this region, 5'-CATATGG, was converted to 5'-CCCATGG in pBT430.
Plasmid DNA containing a cDNA may be appropriately digested to release a nucleic acid fragment encoding the protein. This fragment may then be purified on a 1 % low melting agarose gel. Buffer and agarose contain 10 ~,g/ml ethidium bromide for visualization of the DNA fragment. The fragment can then be purified from the agarose gel by digestion with GELaseT"" (Epicentre Technologies, Madison, WI) according to the manufacturer's instructions, ethanol precipitated, dried and resuspended in 20 ~,L of water. Appropriate oligonucleotide adapters may be ligated to the fragment using T4 DNA ligase (New England Biolabs (NEB), Beverly, MA). The fragment containing the ligated adapters can be purified from the excess adapters using low melting agarose as described above. The vector pBT430 is digested, dephosphorylated with alkaline phosphatase (NEB) and deproteinized with phenol/chloroform as described above. The prepared vector pBT430 and fragment can then be ligated at 16°C for 15 hours followed by transformation into DH5 electrocompetent cells (GIBCO BRL). Transformants can be selected on agar plates containing LB media and 100 pg/mL ampicillin. Transformants containing the gene encoding the instant polypeptides are then screened for the correct orientation with respect to the T7 promoter by restriction enzyme analysis.
For high level expression, a plasmid clone with the cDNA insert in the correct orientation relative to the T7 promoter can be transformed into E. coli strain BL21 (DE3) (Studier et al. (1986) J. Mol. Biol. 189:113-130). Cultures are grown in LB medium containing ampicillin (100 mg/L) at 25°C. At an optical density at 600 nm of approximately 1, IPTG (isopropylthio-~i-galactoside, the inducer) can be added to a final concentration of 0.4 mM and incubation can be continued for 3 h at 25°. Cells are then harvested by centrifugation and re-suspended in 50 p.L of 50 mM Tris-HCI at pH 8.0 containing 0.1 mM DTT and 0.2 mM phenyl methylsulfonyl fluoride. A small amount of 1 mm glass beads can be added and the mixture sonicated 3 times for about 5 seconds each time with a microprobe sonicator. The mixture is centrifuged and the protein concentration of the supernatant determined. One p,g of protein from the soluble fraction of the culture can be separated by SDS-polyacrylamide gel electrophoresis. Gels can be observed for protein bands migrating at the expected molecular weight.

SEQUENCE LISTING
<110> E.I. du Pont de Nemours and Company <l20> FLORAL DEVELOPME1QT GENES
<130> BB1461 PCT
<140>
<141>
<150> 60/253,415 <151> 2000-11-28 <160> 62 <170> Microsoft Office 97 <210> 1 <211> 492 <212> DNA
<213> Alstroemeria caryophylla <400> 1 attgatttct agctagctcc tttgcttgca atagatatat aatgagcgga gaaagcgaaa 60 ccctggtgat tggtagggtg gtgggggacg tgttggaccc ctacactaaa accacggcgc 120 tcaggatcag gtatggatcg aaagaggtga cgtgcgggca cgagctaaag ccatcgcagg 180 tcgtcataca gccaagggtg gaggttggag ggaaggatct caggaccttt tacacacttg 240 tgatggtaga ccctgatgct ccgagcccaa gcaacccaca ccttagggcg tatctacatt 300 ggctggtgac tgacctcccg ggaactactg gagctagctt cgggcaagag gtgatgaggt 360 acgagagccc aaggccaaca ttagggattc accgcttcgt cttcgtgctg ttccggcagc 420 tcgggcggca gacggtgcag gtgcccaccc ccgggaggcg ccagaacttc aacacaaggg 480 gctttgcaag ag 492 <210> 2 <211> 150 <212> PRT
<213> Alstroemeria caryophylla <400> 2 Met Ser Gly Glu Ser Glu Thr Leu Val Ile G1y Arg Val Val Gly Asp Val Leu Asp Pro Tyr Thr Lys Thr Thr Ala Leu Arg Ile Arg Tyr Gly Ser Lys Glu Val Thr Cys Gly His Glu Leu Lys Pro Ser Gln Val Val Tle Gln Pro Arg Val Glu Val Gly Gly Lys Asp Leu Arg Thr Phe Tyr Thr Leu Val Met Val Asp Pro Asp Ala Pro Ser Pro Ser Asn Pro His ~5 70 75 80 Leu Arg Ala Tyr Leu His Trp Leu Val Thr Asp Leu Pro Gly Thr Thr Gly Ala Ser Phe Gly Gln Glu Val Met Arg Tyr Glu Ser Pro Arg Pro Thr Leu Gly I1e His Arg Phe Val Phe Val Leu Phe Arg Gln Leu Gly Arg Gln Thr Val Gln Val Pro Thr Pro Gly Arg Arg Gln Asn Phe Asn Thr Arg Gly Phe Ala Arg <210> 3 <211> 615 <212> DNA
<213> Momordica charantia <220>
<221> unsure <222> (567) <223> n = A, C, G, or T
<220>
<221> unsure <222> (591) <223> n = A, C, G, or T
<220>
<221> unsure <222> (593) <223> n = A, C, G, or T
<400> 3 gtttgtggcg ctagcctttg tgatctctca tggctatgtc cgtggaccct ctggtggtcg 60 gccgagtgat cggagacgtg gtcgacatgt ttgtgccaac tgctaacctg gcagtctact 120 tcaactccaa acatgttact aatggttgcg acattaagcc ttctcttgcg gttaacccac 180 caaggctcgt cattccgggc catcctcgcg acctttacac tttggtgatg acagatccag 240 atgctccgag tcctagcgaa cctcatatga gagaatgggt ccattggata attgtagaca 300 ttcccggagg ctcaacaatg acccaaggga aggagattct gccgtacacc ggcccacgtc 360 cacccatcgg aatccaccgc tacatccttt tactgttcaa gcaaaagggt cctgtggggt 420 tgatcgagca accaccgagc cgcgcaaact tcagcactcg cctgtttgct aagcacctcg 480 acctggacct gccggtggcg gccacctact tcaactctca gaaggaacca gccaccaaaa 540 agttcgcaat gtaatctgaa ccaagtngtc aacccaaacc aaaaaaaaat ngnagtcatc 600 cacgggcaaa atttc ' 615 <210> 4 <211> 174 <212> PRT
<213> Momordica charantia <400> 4 Met Ala Met Ser Val Asp Pro Leu Val Val Gly Arg Val Ile Gly Asp l 5 10 l5 Val Val Asp Met Phe Val Pro Thr Ala Asn Leu Ala Va1 Tyr Phe Asn Ser Lys His Val Thr Asn Gly Cys Asp Ile Lys Pro Ser Leu Ala Val Asn Pro Pro Arg Leu Val Ile Pro Gly His Pro Arg Asp Leu Tyr Thr Leu Val Met Thr Asp Pro Asp Ala Pro Ser Pro Ser Glu Pro His Met Arg Glu Trp Val His Trp Ile Ile Val Asp Ile Pro Gly Gly Ser Thr Met Thr Gln Gly Lys Glu Ile Leu Pro Tyr Thr Gly Pro Arg Pro Pro Ile Gly Ile His Arg Tyr Ile Leu Leu Leu Phe Lys Gln Lys Gly Pro Val Gly Leu Ile Glu Gln Pro Pro Ser Arg Ala Asn Phe Ser Thr Arg Leu Phe Ala Lys His Leu Asp Leu Asp Leu Pro Val Ala A1a Thr Tyr Phe Asn Ser Gln Lys Glu Pro Ala Thr Lys Lys Phe Ala Met <210> 5 <211> 859 <212> DNA
<213> Impatiens balsamia <400> 5 gcacgagagc tcatctttcc cagttttgct cccccttttg gctaaaatgt ctcagatctc 60 tgcctccatt gaccctctca ttatgtgcag aatcatagga gatgtggttg atgtgtttgt 120 tcccaccacg gctatgaatg tctactttgg gaacaagcat gttaccaatg gctgtaacat 180 caagccttcc atggcttatg atgccccaaa tgtcactatt tctgggatgc ctcatgagct 240 ttacactctt gtgatgacag atccagatgc tccaagtcca agtgagccct ccatgaggga 300 atgggtccac tgggttgtga ccaacattcc cgggggcagc agtgcggctc aagggaaaga 360 gctggtgtcc tacatgggtc catgcccagc tattgggatt catcgctaca ttttgatcct 420 gtaccgtcag tccatatatg tggaccagaa cattgagaag cctaacatca taaccagggc 480 caacttcagc accagggctt tctctcatca cctttgcctg ggagttcctg tggccactgt 540 ttacttcaat gctcagaagg agcccctgaa ccagcgcaag aatgtgtgaa ggaacggccc 600 tggagcggcg agagaacgtg gagcaagcta cttcgtttgt cttttccttt tagtataagt 660 aatatcatgc attagcatga ccctaagaat aattgatgtt gtgggatatg tgtgttttac 720 catctctttg tttggttatg ttatgcattt ccctttaggc tttaatgttt gtatgcattt 780 ccctttggct taatatttca atgcatttcc ctcaaaaaaa aaaaaaaaaa aaaaaaaaaa 840 aaaaaaaaaa aaaaaaaaa 859 <210> 6 <211> 180 <212> PRT
<213> Tmpatiens balsamia <400> 6 Met Ser Gln Ile Ser Ala Ser Ile Asp Pro Leu Ile Met Cys Arg Ile Ile Gly Asp Val Val Asp Val Phe Val Pro Thr Thr Ala Met Asn Val Tyr Phe Gly Asn Lys His Val Thr Asn Gly Cys Asn Ile Lys Pro Ser Met Ala Tyr Asp A1a Pro Asn Val Thr Ile Sex Gly Met Pro His Glu Leu Tyr Thr Leu Val Met Thr Asp Pro Asp Ala Pro Ser Pro Ser Glu Pro Ser Met Arg Glu Trp Val His Trp Val Val Thr Asn Ile Pro Gly Gly Ser Ser Ala A1a Gln Gly Lys G1u Leu Val Ser Tyr Met Gly Pro Cys Pro Ala Ile Gly Ile His Arg Tyr Ile Leu Ile Leu Tyr Arg Gln 1l5 120 125 Ser Ile Tyr Val Asp Gln Asn Ile Glu Lys Pro Asn Ile Ile Thr Arg Ala Asn Phe Ser Thr Arg Ala Phe Ser His His Leu Cys Leu Gly Val Pro Val Ala Thr Val Tyr Phe Asn Ala Gln Lys Glu Pro Leu Asn Gln Arg Lys Asn Val l80 <210> 7 <211> 1078 <212> DNA
<213> Zea mays <400> 7 ggcgcgccgg ccggccggtc gattccccca ctccactcgc cgcccgcggc tgggctgcgc 60 tgcgcatcga cgacggacga cgacacaatc acccccaccc cccgtccaat cagcagcgga 120 cgagggacga ccacggcccc ccgtctgccg cacgcgcgcc cgctctgcca gctgctgcta 180 ctactgctaa acctcgccca ccagtcgcgt gaggaaatag caacctgctg agctcgctcg 240 ttcgctcgct cgcctgcctt cttccctggg caagctagct agctaggatc gaggaggagc 300 tctgcccggc catgcagcgt ggggatccgc tggtggtggg ccgcatcatc ggcgacgtgg 360 tggacccctt cgtgcgccgg gtgccgctcc gcgtcgccta cgccgcgcgc gaggtctcca 420 acggctgcga gctcaggccc tccgccatcg ccgaccagcc gcgcgtcgag gtcggcggac 480 ccgacatgcg caccttctac accctcgtga tggtagatcc tgatgcgccg agccccagcg 540 atcccaacct cagggagtac ctgcactggc tggtcactga tattccggcg acgactggag 600 tatcttttgg gaccgaggtc gtgtgctacg agagcccacg gccggtgctg gggatccacc 660 gggtcgtgtt tctgctcttc cagcagctcg gccggcagac ggtgtacgcc ccggggtggc 720 ggcagaactt cagcacccgc gacttcgccg agctctacaa cctcggcttg ccggtcgccg 780 ccgtctactt caactgccag agggagtccg gaaccggtgg gagaagaatg tgatctcgac 840 ccggccgggt ggaaattaat aagatgacgg gtaatcgggt atatgtatat atttatatat 900 atatgtatat gtacgtgtat ttgatctggt ggcctttggt tatattgggt ggggtgtatt 960 tgatatatta tctgtggcag attggcgcat tctctggcgc atatttgata gctacatgta 1020 tctatttata cagatataaa gcgagcaata atatgcatat gagagggttc agccaaaa 1078 <210> 8 <211> 173 <212> PRT
<213> Zea mays <400> 8 Met Gln Arg Gly Asp Pro Leu Val Val Gly Arg Ile Ile Gly Asp Val Val Asp Pro Phe Val Arg Arg Val Pro Leu Arg Val Ala Tyr A1a Ala Arg Glu Val Ser Asn Gly Cys Glu Leu Arg Pro Ser Ala Ile Ala Asp Gln Pro Arg Val Glu Val Gly Gly Pro Asp Met Arg Thr Phe Tyr Thr Leu Val Met Val Asp Pro Asp Ala Pro Ser Pro Ser Asp Pro Asn Leu Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Ala Thr Thr Gly Val Ser Phe Gly Thr Glu Val Val Cys Tyr Glu Ser Pro Arg Pro Val Leu Gly I1e His Arg Val Val Phe Leu Leu Phe Gln Gln Leu Gly Arg Gln Thr Va1 Tyr Ala Pro Gly Trp Arg Gln Asn Phe Sex Thr Arg Asp Phe Ala Glu Leu Tyr Asn Leu Gly Leu Pro Val Ala Ala Val Tyr Phe 145 l50 155 160 Asn Cys Gln Arg Glu Ser Gly Thr Gly Gly Arg Arg Met <210> 9 <211> 929 <212> DNA
<213> Zea mays <400> 9 gcacgagaag aaaccgaacg agggtttagc tagcaaaata aacagaagca agcaagctag 60 ctagagctaa ggatcgagat cgagatcgac cgaccgacga cgatcagcat ggcgcgcttc 120 gtggatccgc tggtggtggg gcgggtgatc ggcgaggtgg tggacctgtt cgtgccttcc 180 atctccatga ccgtcgccta tgatggctcc aaggacatca gcaacggctg cctcctcaag 240 ccgtccgcca ccgccgcgcc gccgctcgtc cgcatctccg gccgccgcaa cgacctctac 300 acgctgatca tgacggaccc cgatgcgcct agccccagca acccgaccat gagagagtac 360 ctccactgga tagtgattaa cataccagga ggaacagatg ctactaaagg tgaggaggtg 420 gtggagtaca tgggcccgcg gccgccggtg ggcatccacc gctacgtgct ggtgctgttc 480 gagcagaaga cgcgcgtgca cgcggaggcc cccggcgacc gcgccaactt caagacgcgc 540 gcgttcgcgg cggcgcacga gctcggcctc cccactgccg tcgtctactt caacgcgcag 600 aaggagcccg ccagccgccg ccgctagcta gcagctcctc tctgaggcat gccagatgca 660 tgcgtgtgcg tgcaggtgca accaccgcac tgccggcggc tacgtatgac cggtgaataa 720 aaagttttac tgcaccgtaa gcatgctcgc cctgttgcta ttggtatatg ttagcagtgt 780 ggcagtctgt atgtagtagc tattcgcttg catctatgca ctctatgtta gtatgcgtac 840 gtgtggttcc ggaacttttg gagtcttatc taaatactat tgagtaaaac tccagtagtt 900 cactcttaaa caaaaaaaaa aaaaaaaaa 929 <210> 10 <211> 172 <212> PRT
<2l3> Zea mays <400> 10 Met A1a Arg Phe Val Asp Pro Leu Val Val Gly Arg Val Ile Gly Glu Val Val Asp Leu Phe Val Pro Ser Ile Ser Met Thr Val Ala Tyr Asp Gly Ser Lys Asp Ile Ser Asn Gly Cys Leu Leu Lys Pro Ser Ala Thr Ala Ala Pro Pro Leu Val Arg Ile Ser Gly Arg Arg Asn Asp Leu Tyr Thr Leu Ile Met Thr Asp Pro Asp Ala Pro Ser Pro Ser Asn Pro Thr Met Arg Glu Tyr Leu His Trp Ile Val Ile Asn Ile Pro Gly Gly Thr Asp Ala Thr Lys Gly Glu Glu Val Val Glu Tyr Met Gly Pro Arg Pro Pro Val Gly Ile His Arg Tyr Val Leu Val Leu Phe Glu Gln Lys Thr Arg Val His Ala Glu A1a Pro Gly Asp Arg Ala Asn Phe Lys Thr Arg Ala Phe Ala Ala Ala His Glu Leu Gly Leu Pro Thr Ala Val Val Tyr Phe Asn Ala Gln Lys Glu Pro Ala Ser Arg Arg Arg <210> 11 <211> 899 <212> DNA
<213> Zea mays <400> 11 ttcaagccaa gttagcttgc ctcgaagatt gccaatcata gctagccatg tcaagggacc 60 cacttgttgt aggcaacgta gttggagata tcttggaccc atttatcaaa tcagcatcac 120 tcagagtcct atacaacaat agagaactga ctaatggatc tgagttcagg ccatcgcaag 180 tagcttatga accaaggatt gagattgctg gatatgacat gaggaccctt tacactttgg 240 taatggtgga tcctgactca ccaagtccaa gcaatccaac aaaaagagag taccttcact 300 ggttggtgac agatattcca gaatcaacag atgtgagctt tggaaatgag gtagtaagct 360 atgaaagccc aaagccaagt gctggaatac atcgcttcgt ctttgttctg gtccgccaat 420 ctgtcaggca aactatttat gcgccaggat ggagacaaaa tttcaacaca agagacttct 480 cagcactcta taatctagga ccacctgtgg cctcagtgtt cttcaactgc caaagggaga 540 atgggtgcgg tggcagacga tatattagat gatactcact ccgttctttt ttatttgtcg 600 cgttttagtt taaaaataaa ctagcggacg acaaatattc gagaacggag gtagtattag 660 aataacctcc tctacatgag gactgacgga attctgtatg aggccaagca caccgaatgg 720 gtagtaaacg ctggacctta atttctagac tactttccca cctctacaag atttgactat 780 gctagaaacg aatttcactt accatgtgaa atgtgataaa tatattccaa ctatatgttc 840 ctgcctcctt gataatgaat actactcagc attggttttg taaaaaaaaa aaaaaaaaa 899 <210> 12 <211> 174 <2l2> PRT
<213> Zea mays <400> 12 Met Ser Arg Asp Pro Leu Val Val Gly Asn Val Val Gly Asp Ile Leu Asp Pro Phe Ile Lys Ser Ala Ser Leu Arg Val Leu Tyr Asn Asn Arg Glu Leu Thr Asn Gly Ser Glu Phe Arg Pro Ser Gln Val Ala Tyr Glu Pro Arg Ile Glu Ile Ala Gly Tyr Asp Met Arg Thr Leu Tyr Thr Leu Val Met Val Asp Pro Asp Ser Pro Ser Pro Ser Asn Pro Thr Lys Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Glu Ser Thr Asp Val Ser Phe Gly Asn Glu Val Val Ser Tyr Glu Ser Pro Lys Pro Ser Ala Gly Ile His Arg Phe Val Phe Val Leu Val Arg Gln Ser Val Arg Gln l15 120 125 Thr Ile Tyr Ala Pro Gly Trp Arg Gln Asn Phe Asn Thr Arg Asp Phe Ser Ala Leu Tyr Asn Leu Gly Pro Pro Val Ala Ser Val Phe,Phe Asn Cys Gln Arg Glu Asn Gly Cys Gly Gly Arg Arg Tyr Ile Arg <210> 13 <211> 893 <212> DNA
<213> Zea mays <400> 13 ggccgtagat agtaagtaga tcacgcagcg cagtagctct ggattaatta ataataattg 60 ctcgtgcgtg tgtccagagc cgccatggct gcccatgtgg acccgctggt tgtggggagg 120 gtgatcggcg acgtggtgga cttgttcgtg ccgacggtgg ccgtgtcggc gcgcttcggc 180 gccaaggacc tcaccaacgg ctgcgagatc aagccatccg tcgccgcggc cgctcccgcc 240 gtcctcatcg ccggcagggc caacgacctc ttcaccctgg ttatgactga cccagatgct 300 ccgagcccta gcgagccaac gatgagggag ttgctccact ggctggtggt taacatacca 360 ggtggagcag atgcttctca aggcggtgag acggtggtgc cgtacgtggg cccgcgcccg 420 ccggtgggta tccaccgcta cgtgctggtg gtgtaccagc agaaggcccg cgtcacggct 480 ccgccgtcgc tggcgccggc gacggaggcg acgcgcgcac ggttcagcaa ccgcgccttc 540 gccgaccgcc atgacctagg cctccctgtc gccgccatgt tcttcaacgc gcagaaggag 600 acagctagtc gccgccgcca ctactgagac aggctgatcg tcgtccaacg gcaattacgt 660 acccagcaaa gcttaagcca gccgctgcag tcactcatct catcgagaag aagacaatct 720 tcctagtcgc tgttcttgcc aagtactagt accttgttaa ttattatgta agctaaaccc 780 gtgtgcctgt gattatattg ggacgtgtct cgctttaata caaccgctca acttgtggcg 840 tttaattatt ttatttatta gatataccaa ggtgtcatca agtcacttgc ctt 893 <210> 14 <211> 180 <212> PRT
<213> Zea mays <400> 14 Met Ala Ala His Val Asp Pro Leu Val Val Gly Arg Val Ile Gly Asp Val Val Asp Leu Phe Val Pro Thr Val Ala Val Ser Ala Arg Phe Gly Ala Lys Asp Leu Thr Asn Gly Cys Glu Ile Lys Pro Ser Val Ala Ala Ala Ala Pro Ala Val Leu Ile Ala Gly Arg Ala Asn Asp Leu Phe Thr Leu Val Met Thr Asp Pro Asp Ala Pro Ser Pro Ser G1u Pro Thr Met Arg Glu Leu Leu His Trp Leu Val Val Asn Ile Pro Gly Gly Ala Asp Ala Ser Gln Gly Gly Glu Thr Val Va1 Pro Tyr Val Gly Pro Arg Pro Pro Val Gly Ile His Arg Tyr Val Leu Val Va1 Tyr Gln G1n Lys Ala Arg Val Thr Ala Pro Pro Ser Leu Ala Pro Ala Thr Glu Ala Thr Arg 130 135 l40 Ala Arg Phe Ser Asn Arg Ala Phe Ala Asp Arg His Asp Leu Gly Leu l45 150 155 160 Pro Val Ala Ala Met Phe Phe Asn Ala Gln Lys Glu Thr Ala Ser Arg Arg Arg His Tyr <210> 15 <211> 837 <212> DNA
<213> Zea mays <400> 15 ccacgcgtcc ggtactgtga gagtaaggct aaagtcgccg gataatataa gaccagcaat 60 aacaagctag tttgccctcg ttctccaaca aaatgtctga tgtggagccg ctggttctgg 120 ctcatgtcat acgagatgtg ttggattcat ttgcaccaag tatcgggctc agaataacct 180 acaacagcag gttacttcta tcaggtgttg agctgaaacc atccgcggtt gtgaataagc 240 caagagttga tgttgggggc accgacctca gggtgttcta cacattggta ttagtggatc 300 cagatgcccc aagcccaagc aatccatcac tgagggagta tctgcactgg atggtgatag 360 acattcctgg aacaactgga gccagctttg gtcaggagct catgttttac gagaggccag 420 agccgaggtc cggcatacac cgcatggtgt tcgtgctgtt ccggcagctc ggcaggggga 480 cggtgtttgc accagacatg cggcacaact tcaactgcaa gagcttcgcc cgtcagtacc 540 acctggacgt cgtggctgcc acgtatttca actgccaaag ggaggcagga tccgggggca 600 gaaggttcag gccggagagc tcgtaaggaa tgaagcatgc acagaagaag actgcagcgc 660 tttcgcatgc atatgatcta tcgtcgtcct gcggaatata tatatagtaa ccgttgttat 720 atggaataat gtgcatgaaa ttggtatcag atgcaccgac ccgtacgtac gtaattaatg 780 tttgttatta cacgcagaca tataatatac atactcattc acaaaaaaaa aaaaaaa 837 <210> 16 <211> 177 <212> PRT
<213> Zea mays <400> 16 Met Ser Asp Val Glu Pro Leu Val Leu Ala His Val Ile Arg Asp Val Leu Asp Ser Phe Ala Pro Ser I1e Gly Leu Arg Ile Thr Tyr Asn Ser Arg Leu Leu Leu Ser Gly Val Glu Leu Lys Pro Ser Ala Va1 Val Asn Lys Pro Arg Val Asp Val Gly Gly Thr Asp Leu Arg Val Phe Tyr Thr Leu Val Leu Val Asp Pro Asp Ala Pro Ser Pro Ser Asn Pro Ser Leu Arg Glu Tyr Leu His Trp Met Val Ile Asp Tle Pro Gly Thr Thr Gly Ala Ser Phe Gly Gln Glu Leu Met Phe Tyr Glu Arg Pro Glu Pro Arg Ser Gly Ile His Arg Met Val Phe Val Leu Phe Arg Gln Leu Gly Arg Gly Thr Val Phe Ala Pro Asp Met Arg His Asn Phe Asn Cys Lys Ser Phe Ala Arg Gln Tyr His Leu Asp Val Val Ala Ala Thr Tyr Phe Asn Cys Gln Arg Glu Ala Gly Ser Gly Gly Arg Arg Phe Arg Pro Glu Ser Ser <210> 17 <211> 1191 <212> DNA
<213> Zea mays <400> 17 ccacgcgtcc ggtagtacct tggccaaacg acttagctat caagctcgac cgaagctaag 60 ctaccaagct agtagccttc ttggtcacgt accggccgtt gttgattgca gcggtcaagc 120 acacacaagc taggcagcta gctagctaga gctagggtcg tcggatagat cgacatggcc 180 ggcagggaca gggagccgct ggtggttggt agggtggtcg gcgacgtgct ggaccccttc 240 gtccggacca ccaacctcag ggtcagctac ggggccagga ccgtgtccaa cggctgcgag 300 ctcaagccgt ccatggtggt gcaccagccc agggtcgagg tcgggggacc tgacatgagg 360 accttctaca ccctcgtgat ggtggacccg gatgctccga gcccaagcga cccgaacctt 420 agggagtacc tacactggct ggtgacggat attccgggaa ctactggggc agcatttggg 480 caagaggtga tctgctacga gagccctcgg ccgaccatgg ggatccaccg cttcgtgctg 540 gtgctgttcc agcagctggg gcggcagacg gtgtacgccc cgggctggcg ccagaacttc 600 aacaccaggg acttcgccga gctctacaac ctgggcccgc ccgtcgccgc cgtctacttc 660 aactgccagc gtgaggccgg ctctgggggc aggaggatgt actcgtgatc ggatgcatgg 720 ttacatacca tgcacactac tcactccatc gtctccatac atgtagacgg acgatggtgc 780 atgcatcgat cgtcaactac tcaacaatta cgaactagaa atacacgcgt atatatacat 840 atataaatat gcatatatac cggtactgta catgtcgccg tacacgcgca ggtggctgct 900 gctagcttgc tataccggcc ggtggtactg agcaggcagc atgcgctata tacttgcttg 960 gcgacgacgt gcagtgtgtg tatacaataa tgagcggccg gccggctagc agggcgacga 1020 gccgtggctt tagcaataca tataccatgc aggcatgtgt gtgtgcagtg cgtgccaagg 1080 tacggtaacg tattaattat tgtgcacata cacatatgta tacgtacata tgcgtaaata 1140 tgaatgtgta cgtatacata tgcatgctgg ttaattaaaa aaaaaaaaaa a 1191 <210> 18 <211> 177 <212> PRT
<213> Zea mays <400> 18 Met Ala Gly Arg Asp Arg Glu Pro Leu Val Val Gly Arg Va1 Va1 Gly 1 5 l0 15 Asp Val Leu Asp Pro Phe Val Arg Thr Thr Asn Leu Arg Val Ser Tyr Gly Ala Arg Thr Val Ser Asn Gly Cys Glu Leu Lys Pro Ser Met Val Val His Gln Pro Arg Val Glu Val Gly Gly Pro Asp Met Arg Thr Phe Tyr Thr Leu Val Met Val Asp Pro Asp Ala Pro Ser Pro Ser Asp Pro Asn Leu Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Gly Thr Thr Gly Ala Ala Phe Gly Gln Glu Val Ile Cys Tyr Glu Ser Pro Arg Pro Thr Met Gly Ile His Arg Phe Val Leu Val Leu Phe Gln Gln Leu Gly Arg Gln Thr Val Tyr Ala Pro Gly Trp Arg Gln Asn Phe Asn Thr Arg Asp Phe Ala Glu Leu Tyr Asn Leu Gly Pro Pro Val A1a Ala Val Tyr Phe Asn Cys Gln Arg Glu Ala Gly Ser Gly Gly Arg Arg Met Tyr Ser <210> 19 <211> 902 <212> DNA
<213> Zea mays <400> 19 ccacgcgtcc ggtattcttg agtgcattcg cttgctccat tcagtcagag cattccttgt 60 gcaaaattca aatacctgtc acaccaacca tgtctaggtc tgtggagcct ctcatagtcg 120 ggcgggtgat tggagaagtt ctcgactcct ttaacccatg tgtcaagatg atagtaacct 180 acaactcaaa caaacttgta ttcaatggcc atgagatcta cccatcagca attgtatcta 240 aacctagggt agaggttcaa gggggtgatt tgcggtcttt cttcacattg gttatgacag 300 acccagatgt tccaggacca agtgatccat atctaaggga gcaccttcat tggatCgtga 360 ctgatatacc tgggacaaca gatgcctcct ttgggcgaga ggtcataagc tatgagagcc 420 caagticctaa catcggtatc cacaggttca tttttgtgct cttcaagcag aagggtaggc 480 aaactgtaac cgtgccatcc ttcagagatc atttcaacac ccggcagttt gctgaggaaa 540 atgaccttgg cctcccagta gctgctgtct acttcaatgc acagagagaa actgcagcta 600 ggagacgttg aaaattccag ctcttattgt ccacctgatg ataataaagg ccttctgatc 660 ttctttctag gaagccaatg aacttattct acattaaatt ctcctgagcc ctaccgtata 720 aataaaccag atgcgttttg ctgattgtat tagtattaga atgctttgta cgtggcaaga 780 atgagaatta caaatggtca atgcttgtgg taaaatttga tgtgtaaaaa aaaaaaaaaa 840 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 900 gg 902 <210> 20 <211> 173 <212> PRT
<213> Zea mays <400> 20 Met Ser Arg Ser Val Glu Pro Leu Ile Val Gly Arg Val Ile Gly Glu Val Leu Asp Ser Phe Asn Pro Cys Val Lys Met Ile Val Thr Tyr Asn Ser Asn Lys Leu Val Phe Asn Gly His Glu Ile Tyr Pro Ser Ala 21e Val Ser Lys Pro Arg Val Glu Val Gln Gly Gly Asp Leu Arg Ser Phe Phe Thr Leu Val Met Thr Asp Pro Asp Val Pro Gly Pro Ser Asp Pro Tyr Leu Arg Glu His Leu His Trp Ile Val Thr Asp Ile Pro Gly Thr Thr Asp Ala Ser Phe Gly Arg Glu Val Ile Ser Tyr Glu Ser Pro Arg Pro Asn Ile Gly Ile His Arg Phe Ile Phe Val Leu Phe Lys Gln Lys 1l5 120 125 Gly Arg Gln Thr Val Thr Val Pro Ser Phe Arg Asp His Phe Asn Thr Arg Gln Phe A1a Glu Glu Asn Asp Leu Gly Leu Pro Val Ala Ala Val Tyr Phe Asn Ala Gln Arg Glu Thr Ala A1a Arg Arg Arg <2l0> 21 <211> 980 <212> DNA
<213> Zea mays <400> 21 ccacgcgtcc gcgcacatag ggaacagaag ctactagctc cagcacaaaa cacctactgc 60 ttcaactgta ccgttagaca tgtcaagggt gttggagcct ctcattgtgg ggaaagtgat 120 tggtgaggtc ctggaccatt tcaaccccac ggtgaagatg gtggtcacct acaactccaa 180 caagcaggtg ttcaacgggc acgagttctt cccttcggca gtggccgcca agccgcgtgt 240 tgaggtccaa gggggcgacc tcaggtcctt cttcacgttg gtgatgaccg accccgatgt 300 tcctggacct agtgatccat acttgaggga gcaccttcac tggattgtca ctgatattcc 360 tgggactacc gatgcttctt ttgggaaaga ggtggtgagc tacgagatcc caaagccaaa 420 cattggcatc cacaggttca tctttgtgct gttccggcag aagagccggc aagcggtgaa 480 cccgccgtcg tcgaaggacc gcttcagcac ccgccagttc gctgaggaga acgacctcgg 540 cctccccgtc gccgccgtct acttcaacgc gcagcgcgag accgccgccc gccgacgcta 600 accgtacggc tcaacgtacg aaagaagacc atcctacgac gcttgcaatt agctgggcaa 660 gcaaagcttt ttttttcatc ctgagtcgat ctttacgtat gtatgtttgt ttaaataaaa 720 aggtagctaa tcagctgctt ggctgtgacc ccacgagcta gcagctacaa cctactggta 780 catgctgcac attttagctg atttatgaag gtgacaatat gattggtagg gttgcaatgt 840 tgactgggca tagtgtaaca acttaagcaa tggccatggg cgagtacgtg tcgagtggtg 900 aagttgaagg gaagtttata ttaaaagcaa ggccatgtct tgtattacct tgcctaaaaa 960 aaaaaaaaaa aaaaaaaaag 98p <210> 22 <21l> 173 <212> PRT
<213> Zea mays <400> 22 Met Ser Arg Val Leu Glu Pro Leu I1e Val Gly Lys Val Ile Gly Glu Val Leu Asp Asn Phe Asn Pro Thr Val Lys Met Thr A1a Thr Tyr Gly Ala Asn Lys Gln Val Phe Asn Gly His Glu Phe Phe Pro Ser Ala Val Ala Gly Lys Pro Arg Val Glu Val Gln G1y Gly Asp Leu Arg Ser Phe Phe Thr Leu Val Met Thr Asp Pro Asp Val Pro Gly Pro Ser Asp Pro Tyr Leu Arg Glu His Leu His Trp Ile Val Thr Asp Ile Pro Gly Thr Thr Asp Ala Ser Phe Gly Arg Glu Val Val Ser Tyr Glu Ser Pro Arg Pro Asn Ile Gly Ile His Arg Phe Ile Leu Va1 Leu Phe Arg Gln Lys Arg Arg Gln Ala Val Ser Pro Pro Pro Ser Arg Asp Arg Phe Ser Thr Arg Gln Phe Ala Glu Asp Asn Asp Leu Gly Leu Pro Val Ala Ala Val Tyr Phe Asn A1a Gln Arg Glu Thr Ala Ala Arg Arg Arg <210> 23 <211> 405 <212> DNA
<213> Oryza sativa <220>
<221> unsure <222> (346) <223> n = A, C, G, or T
<220>
<221> unsure <222> (365) <223> n = A, C, G, or T
<400> 23 ggagagatcg atggcccgtt tcgtggatcc gctggtggtg ggacgggtga tcggggaggt 60 ggtggatttg ttcgttccat ccatctccat gaccgccgcc tacggcgaca gggacatcag 120 CaaCggCtgC CtCgtCCCJCC CatCCgCCgC cgactaccct CCCCtCgtCC gCatCtCCgg 180 ccgccgcaac gacctctaca ccctgatcat gacggacccg gacgcaccta gccctagcga 240 cccatccatg agggagtttc tccactggat cgtggttaac ataccggggg gaacagatgc 300 atctaaaggt gaggagatgg tggagtacat ggggccacgg gcgacngtgg ggataaacaa 360 gtacnttgct ggtgctgtac aacaaaaagc gcgctttctg ggacg 405 <210> 24 <21l> 128 <212> PRT
<213> Oryza sativa <220>
<221> UNSURE
<222> (119) <223> Xaa = any amino acid <400> 24 Met Ala Arg Phe Val Asp Pro Leu Val Val Gly Arg Val Ile Gly Glu 1 5 10 l5 Val Va1 Asp Leu Phe Val Pro Ser Ile Ser Met Thr Ala Ala Tyr Gly Asp Arg Asp Ile Ser Asn Gly Cys Leu Val Arg Pro Ser Ala Ala Asp Tyr Pro Pro Leu Val Arg Ile Ser G1y Arg Arg Asn Asp Leu Tyr Thr Leu Ile Met Thr Asp Pro Asp Ala Pro Ser Pro Ser Asp Pro Ser Met Arg G1u Phe Leu His Trp Ile Val Val Asn Ile Pro Gly Gly Thr Asp Ala Ser Lys Gly Glu Glu Met Val Glu Tyr Met Gly Pro Arg Ala Thr Val Gly Ile Asn Lys Tyr Xaa Ala Gly Ala Val Gln Gln Lys Ala Arg <210> 25 <211> 419 <212> DNA
<213> Oryza sativa <220>
<221> unsure <222> (221) <223> n = A, C, G, or T
<220>
<221> unsure <222> (277) <223> n = A, C, G, or T
<220>
<221> unsure <222> (368) <223> n = A, C, G, or T
<400> 25 cttacaccta atcccagcaa cccaaccttg agggaatacc tgcactggat ggtgactgat 60 atcccatcat cgacggacga tagctttggg cgggagatcg taacatacga aagcccaagc 120 cccaccatgg gcatccaccg catcgtgatg gtgttgtatc agcagcttgg gcgcggcacg 180 gtgttcgcgc cgcagtgggt ccagaacttc aacctgcgca ntttcgcgcg ccgtttcaac 240 ctcggcaagc cggtggccgc catgtacttc aactgcnagc gcccgacagg cacaggtggg 300 aggaggccaa ctgattgatc aatatcgtcg atttcgtctt ctagctcttg tacatgttga 360 gtgttganca atataatggc cactcatgca tatatatata tatatatata tatatatat 419 <210> 26 <211> 105 <212> PRT
<213> Oryza sativa <220>
<221> UNSURE
<222> (74) <223> Xaa = any amino acid <220>
<221> UNSURE
<222> (93) <223> Xaa = any amino acid <400> 26 Leu Thr Pro Asn Pro Ser Asn Pro Thr Leu Arg Glu Tyr Leu His Trp Met Val Thr Asp Ile Pro Ser Ser Thr Asp Asp Ser Phe Gly Arg Glu ~20 25 30 Ile Val Thr Tyr Glu Ser Pro Ser Pro Thr Met Gly Ile His Arg Tle Val Met Va1 Leu Tyr Gln Gln Leu Gly Arg Gly Thr Val Phe Ala Pro Gln Trp Val Gln Asn Phe Asn Leu Arg Xaa Phe Ala Arg Arg Phe Asn 65 70 ' 75 80 Leu Gly Lys Pro Val Ala Ala Met Tyr Phe Asn Cys Xaa Arg Pro Thr Gly Thr Gly Gly Arg Arg Pro Thr Asp <210>27 <211>400 <212>DNA

<213>Oryzasativa <220>

<221>unsure <222>(3) <223>n C, G, or = T
A, <220>

<221>unsure <222>(17l)..(172) <223>n C, G, or = T
A, <220>

<221>unsure <222>(200) <223>n C, G, or = T
A, <220>

<221>unsure <222>(230) <223>n C, G, or = T
A, <220>

<22l>unsure <222>(233) <223>n C, G, or = T
A, <220>
<221> unsure <222> (249) <223> n = A, C, G, or T
<220>
<221> unsure <222> (270) <223> n = A, C, G, or T
<220>
<221> unsure <222> (300) <223> n = A, C, G, or T
<220>
<221> unsure <222> (317) <223> n = A, C, G, or T
<220>
<221> unsure <222> (331) <223> n = A, C, G, or T
<220>
<221> unsure <222> (351) <223> n = A, C, G, or T
<220>
<221> unsure <222> (354)..(355) <223> n = A, C, G, or T
<220>
<221> unsure <222> (36l) <223> n = A, C, G, or T
<220>
<221> unsure <222> (386) <223> n = A, C, G, or T
<220>
<22l> unsure <222> (391)..(392) <223> n = A, C, G, or T
<400> 27 aancacagtc acacacacac agcagaagaa gaagaaaccg aacgagggtt tagctagcaa 60 aataaacaga agcaagcaag ctagctagag ctaaggatcg agatcgagat cgaccgaccg 120 acgacgatca actagcatgg cgcgcttcgt ggatccgctg gtggtggggc nngtgatcgg 180 cgaggtggtg gacctgttcn tgccttccat ctccatgacc gtcgcctatn atngccccaa 240 ggacatcanc aacggctgcc tcctcaagcn gtccgccacc gccgcgccgc cggtcgtccn 300 catctccggc cgccgcnacg acctctacac nctgatgcat gacggacccc natnngccta 360 nccccagcaa cccgaccatg agggantacc nncactggat 400 <210> 28 <211> 87 <212> PRT
<2l3> Oryza sativa <220>
<221> UNSURE
<222> (12) <223> Xaa = any amino acid <220>
<221> UNSURE
<222> (22) <223> Xaa = any amino acid <220>
<221> UNSURE
<222> (32)..(33) <223> Xaa = any amino acid <220>
<221> UNSURE
<222> (38) <223> Xaa = any amino acid <220>
<221> UNSURE
<222> (45) <223> Xaa = any amino acid <220>
<221> UNSURE
<222> (55) <223> Xaa = any amino acid <220>
<221> UNSURE
<222> (61) <223> Xaa = any amino acid <220>
<221> UNSURE
<222> (72)..(73) <223> Xaa = any amino acid <220>
<221> UNSURE
<222> (75) <223> Xaa = any amino acid <220>
<221> UNSURE
<222> (83) <223> Xaa = any amino acid <220>
<221> UNSURE
<222> (85) <223> Xaa = any amino acid <400> 28 Met Ala Arg Phe Val Asp Pro Leu Val Val Gly Xaa Val Ile Gly Glu Val Val Asp Leu Phe Xaa Pro Ser Ile Ser Met Thr Va1 Ala Tyr Xaa Xaa Pro Lys Asp Ile Xaa Asn Gly Cys Leu Leu Lys Xaa Ser Ala Thr Ala Ala Pro Pro Val Val Xaa I1e Ser Gly Arg Arg Xaa Asp Leu Tyr Thr Leu Met Met Thr Asp Pro Xaa Xaa Pro Xaa Pro Ser Asn Pro Thr Met Arg Xaa Tyr Xaa His Trp <210> 29 <211> 1226 <212> DNA
<213> Oryza sativa <400> 29 ggcataagta tatatctgac aaattcagag aaattcagag agtcaccgcg agagcttaag 60 ctagctagct agccggccat ggcatcgcat gtggacccgc tggtggtggg gagggtgatc 120 ggcgacgtgg tggacctgtt cgtgccgacg acggccatgt cggtgcggtt cgggaccaag 180 gacctcacca acggctgcga gatcaagccg tccgtcgccg ccgcgccgcc cgccgtgcag 240 atcgccggca gggtcaacga gctcttcgct ctggtcatga ctgatccaga tgctcctagc 300 cccagcgagc cgactatgag agagtggctt cactggctgg tggttaacat accaggtgga 360 acagatcctt ctcaagggga tgtggtggtg ccgtacatgg ggccacggcc gccggtgggg 420 atccaccgct acgtgatggt gctgttccag cagaaggcgc gcgtggcggc gccgccgccc 480 gacgaggacg ccgcgcgcgc caggttcagc acgcgcgcct tcgccgaccg ccacgacctc 540 ggcctccccg tcgccgccct ctacttcaac gcccagaagg agcccgccaa ccgccgccgc 600 CgCtaCtagC CtCCCtCCCC tCgCt CggCg tcgcccatcc atCCatCCat ggacggcgac 660 ggcgacctag ctagctaata agccatcggt cggccatgct cgccgtccaa actatcatgc 720 accatatcat gtcgtcgttt atgtggttaa ttaattattt ccggcgtttt attactgtgt 780 ggtgccgtac atggggccac ggccgccggt ggggatccac cgctacgtga tggtgctgtt 840 ccagcagaag gcgcgcgtgg cggcgccgcc gcccgacgag gacgccgcgc gcgccaggtt 900 cagcacgcgc gccttcgccg accgccacga cctcggcctc cccgtcgccg ccctctactt 960 caacgcccag aaggagcccg ccaaccgccg ccgccgctac tagcctccct cccctcgctc 1020 ggcgtcgccc atccatccat ccatggacgg cgacggcgac ctagctagct aataagccat 1080 cggtcggcca tgctcgccgt ccaaactatc atgcaccata tcatgtcgtc gtttatgtgg 1140 ttaattaatt atttccggcg ttttattact gtgtggtgat taaaaaaaaa aaaaaaaaaa 1200 aaaaaaaaaa aaaaaaaaaa aaaaaa 1226 <210> 30 <211> 176 <212> PRT
<213> Oryza sativa <400> 30 Met Ala Ser His Val Asp Pro Leu Val Va1 Gly Arg Val Ile Gly Asp Val Val Asp Leu Phe Va1 Pro Thr Thr Ala Met Ser Val Arg Phe Gly Thr Lys Asp Leu Thr Asn Gly Cys Glu Ile Lys Pro Ser Val Ala Ala Ala Pro Pro Ala Val Gln Ile Ala Gly Arg Val Asn Glu Leu Phe Ala Leu Val Met Thr Asp Pro Asp Ala Pro Ser Pro Ser Glu Pro Thr Met Arg Glu Trp Leu His Trp Leu Val Val Asn Ile Pro Gly Gly Thr Asp Pro Ser Gln Gly Asp Val Val Val Pro Tyr Met Gly Pro Arg Pro Pro Val Gly Ile His Arg Tyr Val Met Val Leu Phe Gln Gln Lys Ala Arg Val Ala Ala Pro Pro Pro Asp Glu Asp Ala Ala Arg Ala Arg Phe Ser Thr Arg Ala Phe Ala Asp Arg His Asp Leu Gly Leu Pro Val Ala Ala Leu Tyr Phe Asn Ala Gln Lys Glu Pro Ala Asn Arg Arg Arg Arg Tyr <210> 31 <211> 1295 <212> DNA
<213> Oryza sativa <400> 31 gcacgagatt gcctgcacct agccacatca tatattcaga gagagagctg agagagcagt 60 acaagagtgt atactacact tagcagctca tcagttatta gttcactagt tcagccactg 120 accatcgaat caattcaggt gagataatct tgagatagat atacggccat gtcgagggtg 180 ctggagcctc tcattgtggg gaaggtgatc ggcgaggtgc tggacaactt caaccccacg 240 gtgaagatga cggccaccta cggcgccaac aagcaggtgt tcaacggcca cgagttcttc 300 ccctccgccg tcgccggcaa gccgcgcgtc gaggtccagg gcggcgacct caggtccttc 360 ttcacattgg tgatgactga ccctgatgtg ccagggccta gtgatccata cctgagggag 420 catcttcact ggattgttac tgatattcct gggactactg atgcctcttt tgggagggag 480 gtggtgagct acgagagccc gcggccaaac atcggcatcc acaggttcat cctggtgctg 540 ttccggcaga agcgccggca ggcggtgagc ccgccgccgt cgagggaccg cttcagcacc 600 cgccagttcg ccgaggacaa cgacctcggc ctccccgtcg ccgccgtcta cttcaacgcg 660 cagcgcgaga ccgccgctcg ccgccgctaa tggctaccga cgacggcgac gacgacgacg 720 accctgacac cgcgacgacc gatcttgcat ggacaaaaca atataatcga gcttaattaa 780 ttactactac ttctactggc attttctatt agttttccta tttcccctac attaatttca 840 ctgtcaaata aggcacactg tgattagctg cagctagcta gctttgctcg tgtgtgtgag 900 ctagctagct cgtagctaca gggcaggcct acagactacc agttcgtgct tgtttgcatg 960 cacattatca gattatccta gttgatttgt gaattaatca aggtgatcat aggattgtga 1020 gtgagcaatc gcaatcgcaa tgtgcaagct atggttgact agcagcagtg agagatctct 1080 agctagctac actagttgaa gcaatggcca tccatggcag gagagtccta gcatcccccc 1140 tgcatatatg cctacctact attcaactgc tgttcttcga ttcaattcgc tggtgcttgc 1200 agtgtacttt gtttgatcct gtgatcacta cttcttgcca cttgtttttg taatccgatc 1260 ggtgtcactt tttctgtaaa aaaaaaaaaa aaaaa 1295 <210> 32 <211> 173 <212> PRT
<213> Oryza sativa <400> 32 Met Ser Arg Val Leu Glu Pro Leu Ile Val Gly Lys Val Ile Gly Glu Val Leu Asp Asn Phe Asn Pro Thr Val Lys Met Thr A1a Thr Tyr Gly Ala Asn Lys Gln Val Phe Asn Gly His Glu Phe Phe Pro Ser Ala Val Ala Gly Lys Pro Arg Val Glu Val Gln Gly Gly Asp Leu Arg Ser Phe Phe Thr Leu Val Met Thr Asp Pro Asp Val Pro Gly Pro Ser Asp Pro Tyr Leu Arg Glu His Leu His Trp Ile Val Thr Asp Ile Pro Gly Thr Thr Asp Ala Ser Phe Gly Arg Glu Val Val Ser Tyr Glu Ser Pro Arg Pro Asn Ile Gly Ile His Arg Phe Ile Leu Val Leu Phe Arg Gln Lys Arg Arg Gln Ala Val Ser Pro Pro Pro Ser Arg Asp Arg Phe Ser Thr Arg Gln Phe Ala Glu Asp Asn Asp Leu Gly Leu Pro Val Ala A1a Val Tyr Phe Asn Ala Gln Arg Glu Thr Ala A1a Arg Arg Arg <210> 33 <21l> 567 <212> DNA
<213> Oryza sativa <220>
<221> unsure <222> (401) <223> n = A, C, G, or T
<220>
<221> unsure <222> (411) <223> n = A, C, G, or T
<220>
<221> unsure <222> (428) <223> n = A, C, G, or T

<220>
<221> unsure <222> (435) <223> n = A, C, G, or T
<220>
<221> unsure <222> (437) <223> n = A, C, G, or T
<220>
<221> unsure <222> (455) <223> n = A, C, G, or T
<220>
<221> unsure <222> (475) <223> n = A, C, G, or T
<220>
<221> unsure <222> (524) <223> n = A, C, G, or T
<220>
<221> unsure <222> (536) <223> n = A, C, G, or T
<220>
<221> unsure <222> (543) <223> n = A, C, G, or T
<400> 33 ctcaagttag cttcttagca cagcctcttc ttgctcaact cctgaagatc atcaatcttc 60 actagccatg tcaagggacc cacttgtcgt aggacatgtt gttggggata tcttagaccc 120 attcaacaaa tcagcatcac tcaaggtcct atacaacaac aaggaattaa caaatgggtc 180 tgagctcaaa ccgtcacagg tagcaaatga accaaggatt gaaattgctg gccgcgacat 240 aaggaacctt tacactctgg tgatggtgga tcctgactcg ccaagtccaa gcaacccaac 300 aaaaagagaa taccttcatt gggttgggtg acaagacatt ccaagaatcg gcaaatgcta 360 attatggaaa tgaagtttgt cagttatgaa aagcccaaaa ncaaactgca nggatacatc 420 cgttttgncc ttaanantaa ttccgccaat atgtncaaca agactaatta tgcancaaga 480 tgggggaaca aaatttcaat acaaagagaa tttttccgca acgntaaaac cttggncctc 540 ccngtgggaa caatggttct caaattg 567 <210> 34 <211> 87 <212> PRT
<213> Oryza sativa <400> 34 Met Ser Arg Asp Pro Leu Val Val Gly His Val Val Gly Asp Ile Leu Asp Pro Phe Asn Lys Ser A1a Ser Leu Lys Val Leu Tyr Asn Asn Lys Glu Leu Thr Asn G1y Ser Glu Leu Lys Pro Ser Gln Val Ala Asn Glu Pro Arg Ile Glu Ile Ala Gly Arg Asp Ile Arg Asn Leu Tyr Thr Leu Val Met Val Asp Pro Asp Ser Pro Ser Pro Ser Asn Pro Thr Lys Arg Glu Tyr Leu His Trp Val Gly <210> 35 <211> 850 <212> DNA
<213> Glycine max <400> 35 atggcagcct ccgtggatcc cctagtggtt ggtcgcgtga tcggcgatgt ggtagacatg 60 ttcattcctt cagtcaacat gtccgtttac tttgggtcga agcacgtcac aaatggctgt 120 gacatcaagc catccattgc catcagccct cctaagctca ccctcaccgg caacatggat 180 aacctctaca cactggttat gactgatcct gacgcaccta gccccagtga accaagcatg 240 cgcgagtgga tacattggat cttagttgac atacctggag gaacaaaccc atttcgcgga 300 aaagagattg tttcatatgt gggaccaaga ccacctattg gaatacatcg ctatatcttt 360 gtgttgtttc aacagaaagg acctttaggt cttgtggagc aaccaccaac tcgagcaagc 420 ttcaacactc gttattttgc caggcaattg gacttgggac ttccagtggc cactgtctac 480 ttcaactctc aaaaagaacc tgctgttaag aggcgctgaa tctagctata ttgtaaccat 540 cagtgtctct cttgagatat gcatggttgg aatatacttt taagatatca gaactatata 600 aaatctatga ctagtgcgta aataatgcag ggagagggtg tgtgtaaagt aatatagttg 660 tccaagacat gagtggtgcc gaccatttcc ccggctttga taactttttt tctctatata 720 tatacctctc tttcactcta tcaaatatat aaagttaatc tttattaaaa aaaaaaaaaa 780 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 840 aaaaaaaaaa 850 <210> 36 <211> 172 <212> PRT
<213> Glycine max <400> 36 Met Ala Ala Ser Val Asp Pro Leu Val Val G1y Arg Val Ile Gly Asp Val Val Asp Met Phe Ile Pro Ser Val Asn Met Ser Val Tyr Phe Gly Ser Lys His Val Thr Asn Gly Gys Asp Ile Lys Pro Ser Ile Ala Ile Ser Pro Pro Lys Leu Thr Leu Thr Gly Asn Met Asp Asn Leu Tyr Thr Leu Val Met Thr Asp Pro Asp Ala Pro Ser Pro Ser Glu Pro Ser Met Arg Glu Trp Ile His Trp Ile Leu Val Asp Tle Pro Gly Gly Thr Asn Pro Phe Arg G1y Lys Glu Ile Val Ser Tyr Va1 Gly Pro Arg Pro Pro Ile Gly Ile His Arg Tyr Ile Phe Val Leu Phe Gln Gln Lys Gly Pro Leu Gly Leu Va1 Glu Gln Pro Pro Thr Arg Ala Ser Phe Asn Thr Arg Tyr Phe Ala Arg Gln Leu Asp Leu Gly Leu, Pro Val Ala Thr Val Tyr 145 150 155 l60 Phe Asn Ser Gln Lys Glu Pro Ala Val Lys Arg Arg <210> 37 <211> 969 <212> DNA
<213> Glycine max <400> 37 gcacgagcat aacaattgta ttcctccctt ccttagctcc actacctctt ctctcttcct 60 ccttgttcct tcctcttaca atggcaagaa tgcctttaga gcctctaata gtggggagag 120 tcataggaga agttcttgat tcttttacca caagcacaaa aatgattgtg agttacaaca 180 agaatcaagt ctacaatggc catgaactct tcccttccac tgtcaacacc aagcccaagg 240 ttgagattga gggtggtgat atgaggtcct tctttacact gatcatgact gaccctgatg 300 ttcctggccc tagtgaccct tatctgagag agcacttgca ctggatagtg acagatattc 360 caggcacaac agatgccaca tttgggaaag agttggtgag ctatgagatc ccaaagccta 420 atattgggat ccataggttt gtgtttgtcc tgttcaagca aaagcgtaga cagtgtgtta 480 ctccacccac ttcaagggac cacttcaaca cacgcaaatt cgcagcagag aacgaccttg 540 ccctccctgt ggctgctgtc tacttcaatg cacagaggga aacggctgca agaagacgct 600 agctatagct gctgattttg ccactgcttc aaccaaacta gtattgtatt gtattgaata 660 aagcgataaa aaaaggtaca agtacaagga gtttcagtag tggaattaag ttgatcctca 720 catgtggctt caaataactt gcaggaaggg aagataatta atcattttct agtttgaccc 780 gtgtgtatgc tacgttttat tttactttcc atctgttgtg taaacattat actactacgt 840 gtattattat tacctcgtgg gactactatg aggtgtgatc ttatatatag aaataagagg 900 tttggtacgc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 960 aaaaaaaaa 969 <210> 38 <211> 173 <212> PRT
<213> Glycine max <400> 38 Met Ala Arg Met Pro Leu Glu Pro Leu Ile Val Gly Arg Val Ile Gly Glu Val Leu Asp Ser Phe Thr Thr Ser Thr Lys Met T1e Val Ser Tyr Asn Lys Asn Gln Val Tyr Asn Gly His G1u Leu Phe Pro Ser Thr Val Asn Thr Lys Pro Lys Val Glu Ile Glu Gly Gly Asp Met Arg Ser Phe Phe Thr Leu Ile Met Thr Asp Pro Asp Val Pro Gly Pro Ser Asp Pro Tyr Leu Arg Glu His Leu His Trp Ile Val Thr Asp Ile Pro Gly Thr Thr Asp Ala Thr Phe Gly Lys G1u Leu Val Ser Tyr Glu Ile Pro Lys Pro Asn Ile Gly Ile His Arg Phe Val Phe Val Leu Phe Lys Gln Lys Arg Arg Gln Cys Val Thr Pro Pro Thr Ser Arg Asp His Phe Asn Thr Arg Lys Phe Ala Ala Glu Asn Asp Leu Ala Leu Pro Val Ala Ala Val Tyr Phe Asn Ala Gln Arg Glu Thr A1a Ala Arg Arg Arg <210> 39 <211> 836 <212> DNA
<213> Glycine max <220>
<221> unsure <222> (622) <223> n = A, C, G, or T
<400> 39 gttttagagc atattttctt catttttctt gcattccttc tctttgcaat tgatgtctag 60 gctaatggaa caaccacttg ttgtgggaag agtgatagga gaagtggttg acattttcag 120 cccaagtgta agaatgaatg ttacatattc cactaagcaa gttgctaatg gtcatgagtt 180 aatgccttct actattatgg ccaagccacg cgttgagatt ggtggtgatg acatgaggac 240 tgcttatacc ttgatcatga cagacccaga tgctccaagt cctagtgatc cacatctgag 300 ggaacatctc cactggacgg ttacagatat ccctggcacc acagatgtct cttttggtaa 360 agagatagtg ggctatgaga gtccaaaacc agtaatagga atccacaggt atgtgttcat 420 tttgttcaag cagagaggaa gacagacagt caggcctcct tcttcaagag accatttcaa 480 cacaaggagg ttctcagaag agaatggcct tggcctacca gttgctgtag tttacttcaa 540 tgctcaaaga gagactgccg caagaaggag gtgattcctg aagaagaaga agaagaagaa 600 gaaaggttgc agcagtaaat anaattaatt ttgtttcaac cttaatcatc tcataatgag 660 atttgtttcc tttggttttc ttaggggttg gcatggttga gtaaggaaga taggtgtgtt 720 gatgaatctc tcacacatca atgtttcttg tccatttctt tgggtcacaa cgaggaactg 780 taggtagtgt gtcaacagag tgtatctgat gacttaacgt cactggaaag gtgagg 836 <210> 40 <211> 173 <212> PRT
<213> Glycine max <400> 40 Met Ser Arg Leu Met Glu Gln Pro Leu Val Val Gly Arg Val Ile Gly Glu Val Val Asp Ile Phe Ser Pro Ser Val Arg Met Asn Val Thr Tyr Ser Thr Lys Gln Val Ala Asn Gly His Glu Leu Met Pro Ser Thr Ile Met Ala Lys Pro Arg Val Glu Ile Gly Gly Asp Asp Met Arg Thr Ala Tyr Thr Leu Ile Met Thr Asp Pro Asp Ala Pro Ser Pro Ser Asp Pro His Leu Arg Glu His Leu His Trp Thr Val Thr Asp Ile Pro Gly Thr Thr Asp Val Ser Phe Gly Lys Glu Tle Val Gly Tyr Glu Ser Pro Lys l00 105 110 Pro Val Ile Gly Ile His Arg Tyr Val Phe Ile Leu Phe Lys Gln Arg 115 120 l25 Gly Arg Gln Thr Val Arg Pro Pro Ser Ser Arg Asp His Phe Asn Thr Arg Arg Phe Ser G1u Glu Asn Gly Leu Gly Leu Pro Val Ala Val Val Tyr Phe Asn Ala Gln Arg Glu Thr Ala Ala Arg Arg Arg l65 170 <210> 41 <2l1> 893 <212> DNA
<2l3> Triticum aestivum <400> 41 ttcggcacga ggggagatcc agctagctag ctggctagtt ttgctgttgc tgctcgacct 60 catcgccatc ctccggctat ggcagcccat gtggatcccc ttgtggttgg gagggtgatc 120 ggtgacgtgg tggacatgtt cgtgcccacc atgccggtga ccgtgcgctt cgggacgaag 180 gacctgacga acggctgcga gatcaagccg tccatcgccg acgcggcgcc ctcgatccag 240 atagccggcc gggccggcga tctcttcacc ctggttatga ctgatccgga cgcaccgagc 300 cccagcgagc caaccatgaa ggagtggctt cactggctgg tggttaacat acctggtgga 360 tcagatcctt ctcaagggga ggaggtggtg ccctacatgg gtccgaagcc gccgttgggc 420 atccaccgct acgtgctggt gctgttccag cagaaggcgc gtgtgctggc gccggctccc 480 ggcggcgaca cagcagcgtc ggccatgcgc gcgcggttca gcacccgtgc cttcgcagag 540 cgccatgacc tggggctccc cgtcgccgcc atgtacttca acgcgcagaa ggagccggcc 600 aaccgccgcc gccgctacta gctcgtcgcc gccggccgat caaacccgct gctgcctgct 660 ggtgctgcct gctggtccgt ctgtgtgtgc gtgcatgcgc gcgggcccaa taaattaacc 720 atatcgatct tgtcgttctc atgaacaatc tgggcttgta ttgtgtggta ctctttgttt 780 gttttttctt gcgggtgcgt gtggtactct ttggaccata tacatattta ccgctttctc 840 tcttcgttgt attcattgat tatgtgtgag atccaaaaaa aaaaaaaaaa aac 893 <210> 42 <2l1> 180 <212> PRT
<2l3> Triticum aestivum <400> 42 Met Ala Ala His Val Asp Pro Leu Val Val Gly Arg Val Ile Gly Asp Val Val Asp Met Phe Val Pro Thr Met Pro Val Thr Val Arg Phe Gly Thr Lys Asp Leu Thr Asn Gly Cys Glu Ile Lys Pro Ser Ile Ala Asp Ala Ala Pro Ser Ile Gln Ile Ala Gly Arg Ala Gly Asp Leu Phe Thr Leu Val Met Thr Asp Pro Asp Ala Pro Ser Pro Ser Glu Pro Thr Met Lys Glu Trp Leu His Trp Leu Val Va1 Asn Ile Pro Gly Gly Ser Asp Pro Ser Gln Gly Glu Glu Val Val Pro Tyr Met Gly Pro Lys Pro Pro 100 105 1l0 Leu Gly Ile His Arg Tyr Val Leu Val Leu Phe Gln Gln Lys Ala Arg Val Leu Ala Pro Ala Pro Gly Gly Asp Thr Ala Ala Ser Ala Met Arg Ala Arg Phe Ser Thr Arg A1a Phe Ala Glu Arg His Asp Leu Gly Leu Pro Val Ala Ala Met Tyr Phe Asn Ala Gln Lys Glu Pro Ala Asn Arg Arg Arg Arg Tyr <210> 43 <211> 886 <212> DNA
<213> Triticum aestivum <400> 43 gcacgaggcc gccttcatta acatatcgcc acttgcgccg gcggccggcg gagaagggcg 60 ccagtggtga caggaggaag aagatggtgg ggagcggcat gcatgcccag cgcggggacc 120 cgctggtggt ggggcgcgtg atcggcgacg tggtggaccc gttcgtgcgg cgggtggcgc 180 tgcgggtcgg ctacgcgtcc agggacgtgg ccaacggctg cgagctgagg ccgtccgcca 240 tcgccgaccc gccgcgcgtc gaggtcggcg gcccggacat gcgcaccttc tacacgctgg 300 tgatggtgga tccggatgct ccaagtccca gcgatcccag ccttagggag tacttgcact 360 ggctggtcac cgacatcccg gcgacgacag gagtgtcttt tgggaccgag gtggtgtgct 420 acgagggccc gcggccggtg ctcgggatcc accggctggt gttcctgctc ttccagcagc 480 tgggccgcca gacggtgtac gccccggggt ggcggcagaa cttcagcacc cgcgacttcg 540 ccgagctcta caacctcggc ctgcccgtcg ccgccgtcta cttcaactgc cagagggaga 600 ccggaaccgg cgggagaagg atgtgatgat caactccttg tataatacca gtacttaagt 660 agtataagtg acgacacaag atgatgatga tgatgaggtc gtatgggtgg tggtttatac 720 agggcgaaat ggagaaagaa ttgtaatgtt gaagaaataa taactatgcg tgcgactttt 780 ttgatccgat gccggtgcga tactacaaag attaaaaaga tgttaggatc caaaaaaaaa 840 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa 886 <210> 44 <211> 180 <2l2> PRT
<213> Triticum aestivum <400> 44 Met Val Gly Ser Gly Met His Ala Gln Arg Gly Asp Pro Leu Val Val 1 5 10 l5 Gly Arg Val Ile Gly Asp Val Val Asp Pro Phe Val Arg Arg Val A1a Leu Arg Val Gly Tyr Ala Ser Arg Asp Val Ala Asn Gly Cys Glu Leu Arg Pro Ser Ala Ile Ala Asp Pro Pro Arg Val Glu Val Gly Gly Pro Asp Met Arg Thr Phe Tyr Thr Leu Val Met Val Asp Pro Asp Ala Pro Ser Pro Ser Asp Pro Ser Leu Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Ala Thr Thr Gly Val Ser Phe Gly Thr Glu Val Val Cys Tyr Glu Gly Pro Arg Pro Val Leu Gly Ile His Arg Leu Val Phe Leu 115 l20 125 Leu Phe Gln Gln Leu Gly Arg Gln Thr Val Tyr Ala Pro Gly Trp Arg Gln Asn Phe Ser Thr Arg Asp Phe Ala Glu Leu Tyr Asn Leu Gly Leu Pro Val Ala Ala Val Tyr Phe Asn Cys G1n Arg Glu Thr Gly Thr Gly Gly Arg Arg Met <210> 45 <211> 1257 <212> DNA
<213> Zea mays <400> 45 ctcgctcaga cagctctgct agctgcatcc tcctaactct ccaggtctct ctctcctctc 60 ccaactccca agtcccatcc ggatcgagac gctggaggcg gagcgccccc ccgggacggc 120 ggcggcgacg atggggcgcg gcaagatcga gatcaagcgg atcgagaacg ccaccaaccg 180 ccaggtgacc tactccaagc gccggacggg gatcatgaag aaggcgcgcg agctcaccgt 240 gctctgcgac gcccaggtcg ccatcatcat gttctcctcc accggcaagt accacgagtt 300 ctgcagcccc ggaaccgaca tcaagaccat ctttgaccgg taccagcagg ccatcgggac 360 cagcctatgg atcgagcagt atgagaatat gcagcgcacg ctgagccatc tcaaggacat 420 caatcgtggt ctgcgcacag agattaggca aaggatgggc gaggatctgg acagtctgga 480 cttcgacgag ctgcgcggcc tcgagcaaaa cgtcgacgcg gctctcaagg aggttcgcca 540 taggaagtac catgtgatca gcacgcagac tgatacctac aagaaaaagg tgaagcactc 600 gcacgaggcg tacaagaacc tgcagcagga gctaggcatg cgggaggacc cggcgttcgg 660 gtacgtggac aacacgggcg ccggcgtcgc ctgggacggc gcggcggcgg cgctgggcgg 720 cgccccgccg gacatgtacg ccttccgcgt ggtgcccagc cagcccaacc tgcacggcat 780 ggcctacggc ttccacgacc tccgcctggg ctagcgcatc catcaccatg ctgggtggtg 840 ctgctcgatc ctactgcatg gcaatgcaag ctggttggtt agttcgctca tgcatcgtcc 900 gtcaacaaag caagtaagca atgcaatgca accgaggtac tgtaatagcc aataaaatct 960 actgcatact gcaaacccaa ttactggtag cttagctacc gcgtgtgtac gaatcaaccg 1020 attaattacc gcgcccttag cttgcatgtc gtcgtcgtct gtgcttttgg cgttcgtaga 1080 catgtgtgta ttgtatgcat gggtcctgtt catctgcatc catgcatgtt gtttatgatt 1140 gtaattgttg tgtgaaatgg ctgtactttg ttatgatcac gtgaaattat atctacattc 1200 gtgggaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa 1257 <210> 46 <211> 227 <212> PRT
<213> Zea mays <400> 46 Met Gly Arg Gly Lys Ile Glu Ile Lys Arg Ile Glu Asn Ala Thr Asn Arg Gln Val Thr Tyr Ser Lys Arg Arg Thr Gly Ile Met Lys Lys Ala Arg Glu Leu Thr Val Leu Cys Asp Ala G1n Val Ala Ile Ile Met Phe Ser Ser Thr Gly Lys Tyr His Glu Phe Cys Ser Pro Gly Thr Asp Ile Lys Thr Ile Phe Asp Arg Tyr Gln Gln Ala Ile Gly Thr Ser Leu Trp Ile Glu Gln Tyr Glu Asn Met G1n Arg Thr Leu Ser His Leu Lys Asp Ile Asn Arg Gly Leu Arg Thr Glu Ile Arg Gln Arg Met Gly Glu Asp Leu Asp Ser Leu Asp Phe Asp Glu Leu Arg Gly Leu Glu Gln Asn Val 1l5 120 125 Asp Ala Ala Leu Lys Glu Val Arg His Arg Lys Tyr His Val I1e Ser Thr G1n Thr Asp Thr Tyr Lys Lys Lys Val Lys His Ser His Glu Ala Tyr Lys Asn Leu Gln Gln Glu Leu Gly Met Arg Glu Asp Pro Ala Phe Gly Tyr Val Asp Asn Thr Gly Ala Gly Val Ala Trp Asp Gly Ala Ala Ala Ala Leu Gly Gly Ala Pro Pro Asp Met Tyr Ala Phe Arg Val Val Pro Ser Gln Pro Asn Leu His Gly Met Ala Tyr Gly Phe His Asp Leu Arg Leu Gly <210> 47 <211> 1089 <212> DNA
<213> Zea mays <400> 47 ccacgcgtcc gaccgcaccg gcaccaccac caaccgagcg gctccaggct cctgctcagg 60 aaggggagaa gaggcgagcc ttccttggga agtcgcagga ggagagaagg ggaacaaaga 120 tggggcgcgg caagatcgag atcaagcgga tcgagaactc caccaaccgc caggtgacct 180 tctccaagcg ccgcaacggg atcctcaaga aggcgcggga gatcagcgtg ctctgcgacg 240 ccgaggtcgg cgtcgtcgtc ttctccagcg ccggcaagct ttacgactac tgctccccga 300 agacatcgct atcaaaaatc ctggagaagt accagaccaa ctctggaaag atactgtggg 360 gtgagaagca caagagcctt agtgcagaga ttgaccgtat aaagaaagag aacgacacca 420 tgcagatcga gctcaggcac ctgaaaggtg aagatctaaa ctcgctgcaa cccaaagacc 480 tgatcatgat cgaggaggca cttgataatg gactgacgaa cctgaatgag aaactgatgg 540 agcactggga aaggcgtgtg acaaacacta agatgatgga agacgagaac aaattgctgg 600 ccttcaaact ccaccagcaa gatatcgcgc tgagcggcag catgagagag cttgagctgg 660 gttaccatcc tgaccgggac ctggcggccc agatgccaat caccttccgc gtgcagccca 720 gccatcccaa cttgcaggag aacaattaga ctgctggatg ccctcgttcc actcgccgag 780 gatttcaccc agccaccacc gctggcttgt atgccctcgt gcgctggcaa ctgtatcttt 840 atctttccgg tatgtttgga tgaacgtata atgtgtgtca gtgtcggtcg catgacgtgc 900 cgatgtcgtg catctctctc tctctctgcc agagcagcgg aactctgcac cgtgagtaac 960 ttaattggta ccgtatgatc tgccggacag tgaatagttt atgtgagtgg gtcaaaccat 1020 aatgtgtagt atttgtgtcg aactgtcaat ggcacgtatt tggattttca ctaaaaaaaa 1080 aaaaaaaag 1089 <210> 48 <211> 209 <212> PRT
<213> Zea mays <400> 48 Met Gly Arg Gly Lys Ile Glu Ile Lys Arg Ile G1u Asn Ser Thr Asn Arg Gln Val Thr Phe Ser Lys Arg Arg Asn Gly Ile Leu Lys Lys Ala Arg Glu Ile Ser Val Leu Cys Asp Ala Glu Val Gly Val Val Val Phe Ser Ser Ala Gly Lys Leu Tyr Asp Tyr Cys Ser Pro Lys Thr Ser Leu Ser Lys Ile Leu Glu Lys Tyr Gln Thr Asn Ser Gly Lys Ile Leu Trp Gly Glu Lys His Lys Ser Leu Ser Ala Glu Ile Asp Arg Ile Lys Lys Glu Asn Asp Thr Met Gln Ile Glu Leu Arg His Leu Lys Gly Glu Asp Leu Asn Ser Leu Gln Pro Lys Asp Leu Ile Met Ile Glu Glu Ala Leu Asp Asn Gly Leu Thr Asn Leu Asn Glu Lys Leu Met Glu His Trp Glu Arg Arg Val Thr Asn Thr Lys Met Met Glu Asp Glu Asn Lys Leu Leu Ala Phe Lys Leu His Gln Gln Asp Ile Ala Leu Ser G1y Ser Met Arg Glu Leu G1u Leu Gly Tyr His Pro Asp Arg Asp Leu Ala Ala Gln Met Pro Ile Thr Phe Arg Val Gln Pro Ser His Pro Asn Leu G1n Glu Asn Asn <210> 49 <211> 926 <212> DNA
<213> Glycine max <400> 49 gcacgaggct atggctagag gaaagatcca gatcaagagg atagagaaca acaccaaccg 60 ccaggtcact tactctaaac gacggaatgg ccttttcaag aaggccaacg agcttaccgt 120 tctctgcgat gccaaggttt ctattattat gttctccagc actggaaaac tccaccagta 180 catcagcccc tccacctcaa caaagcagtt cttcgatcaa taccagatga ctctgggagt 240 tgatctctgg aactctcatt acgagaatat gcaagagaac ttgaagaaac tgaaagaggt 300 gaataggaat cttcgtaagg agattaggca gagaatggga gattgtctga acgagctggg 360 catggaagat ctcaagctcc ttgaggaaga aatggacaag gccgccaagg ttgttcgtga 420 gcgtaagtat aaggtgataa caaatcagat tgacacccag aggaaaaagt ttaataacga 480 gaaagaagtg cacaacaggc tcctgcatga cttggatgca aaagcagaag atccacgttt 540 tgcattgata gataatggag gggagtatga gtctgtgatc ggattctcaa atttaggtcc 600 acgcatgttc gcattgagca tacaaccaag ccatcctagt gcccatagcg gaggagcagg 660 ctctgatctt accacttacc ctttactttt ctagtacgca attgcttaag ctctctccat 720 cagaaatacc atattcactc aaatttcaat aagaatgact tgttgcagtt tgtacttaac 780 cacaaaacaa tctcacgaat cttctccgtg gaacgcatgt gtgaattatt caattgcaac 840 tactgttatc tgtattttct ttttgcctaa tcatatacca taaacatgaa gttgtgcttc 900 cttttaaaaa aaaaaaaaaa aaaaaa 926 <210> 50 <211> 227 <2l2> PRT
<213> Glycine max <400> 50 Met Ala Arg Gly Lys Ile Gln Ile Lys Arg Ile Glu Asn Asn Thr Asn l 5 10 15 Arg Gln Val Thr Tyr Ser Lys Arg Arg Asn Gly Leu Phe Lys Lys Ala Asn Glu Leu Thr Va1 Leu Cys Asp Ala Lys Val Ser Ile Ile Met Phe Ser Ser Thr Gly Lys Leu His Gln Tyr Ile Ser Pro Ser Thr Ser Thr Lys Gln Phe Phe Asp Gln Tyr Gln Met Thr Leu Gly Val Asp Leu Trp Asn Ser His Tyr Glu Asn Met Gln Glu Asn Leu Lys Lys Leu Lys Glu Val Asn Arg Asn Leu Arg Lys Glu Ile Arg Gln Arg Met Gly Asp Cys Leu Asn Glu Leu Gly Met Glu Asp Leu Lys Leu Leu Glu Glu Glu Met Asp Lys Ala Ala Lys Val Val Arg Glu Arg Lys Tyr Lys Val Ile Thr Asn Gln Ile Asp Thr Gln Arg Lys Lys Phe Asn Asn Glu Lys Glu Val His Asn Arg Leu Leu His Asp Leu Asp Ala Lys A1a Glu Asp Pro Arg Phe Ala Leu Tle Asp Asn Gly Gly Glu Tyr Glu Ser Val Ile Gly Phe Ser Asn Leu Gly Pro Arg Met Phe Ala Leu Ser Ile Gln Pro Ser His Pro Ser Ala His Ser Gly Gly Ala Gly Ser Asp Leu Thr Thr Tyr Pro Leu Leu Phe <210> 51 <211> 173 <212> PRT
<213> Oryza sativa <400> 51 Met Ser Arg Ser Val Glu Pro Leu Val Val Gly Arg Val Ile Gly Glu 1 5 10 l5 Val Leu Asp Thr Phe Asn Pro Cys Met Lys Met Tle Val Thr Tyr Asn Ser Asn Lys Leu Va1 Phe Asn Gly His Glu Leu Tyr Pro Ser Ala Val Val Ser Lys Pro Arg Val Glu Val Gln Gly Gly Asp Leu Arg Ser Phe Phe Thr Leu Val Met Thr Asp Pro Asp Val Pro Gly Pro Ser Asp Pro Tyr Leu Arg Glu His Leu His Trp Ile Val Thr Asp Ile Pro Gly Thr Thr Asp Ala Ser Phe Gly Arg Glu Val Tle Ser Tyr Glu Ser Pro Lys Pro Asn Ile Gly Ile His Arg Phe Ile Phe Val Leu Phe Lys Gln Lys Arg Arg Gln Thr Val Ile Val Pro Ser Phe Arg Asp His Phe Asn Thr Arg Arg Phe Ala Glu Glu Asn Asp Leu Gly Leu Pro Val Ala Ala Va1 Tyr Phe Asn Ala Gln Arg Glu Thr Ala Ala Arg Arg Arg <210> 52 <211> 224 <212> PRT
<213> Oryza sativa <400> 52 Met Gly Arg Gly Lys Ile Glu Ile Lys Arg Ile Glu Asn Ala Thr Asn Arg Gln Val Thr Tyr Ser Lys Arg Arg Thr G1y Ile Met Lys Lys Ala Arg Glu Leu Thr Val Leu Cys Asp Ala Gln Val Ala Ile Ile Met Phe Ser Ser Thr Gly Lys Tyr His Glu Phe Cys Ser Pro Ser Thr Asp Ile Lys Gly Ile Phe Asp Arg Tyr Gln Gln A1a Ile Gly Thr Ser Leu Trp Ile Glu Gln Tyr Glu Asn Met Gln Arg Thr Leu Ser His Leu Lys Asp Ile Asn Arg Asn Leu Arg Thr Glu Ile Arg Gln Arg Met Gly Glu Asp Leu Asp Gly Leu Glu Phe Asp Glu Leu Arg Gly Leu G1u Gln Asn Val Asp Ala Ala Leu Lys Glu Val Arg His Arg Lys Tyr His Val Ile Thr Thr G1n Thr Glu Thr Tyr Lys Lys Lys Val Lys His Ser Tyr G1u Ala Tyr G1u Thr Leu Gln Gln Glu Leu Gly Leu Arg Glu Glu Pro Ala Phe Gly Phe Val Asp Asn Thr Gly Gly Gly Trp Asp Gly Gly Ala Gly Ala Gly Ala Ala A1a Asp Met Phe Ala Phe Arg Val Val Pro Ser Gln Pro l95 200 205 Asn Leu His Gly Met Ala Tyr Gly Gly Asn His Asp Leu Arg Leu Gly <210> 53 <211> 613 <212> DNA
<213> Zea mays <220>
<221> unsure <222> (613) <223> n = A, C, G or T
<400> 53 tacttctcgg cgtcggcgct gctccgagtg atgtacggcg ggcgcgagat gacctgcggg 60 tcggagctca ggccgtcgca ggtggcgagc gagccgacgg tgcacatcac ggggggccgc 120 gacgggaggc cggtgctcta cacactggtg atgctggacc ccgatgcgcc cagcccaagc 180 aacccctcca agcgggagta tctccattgg ttggtgactg acataccaga aggagctggt 240 gccaatcatg ggaacgaggt ggtggcgtac gagagccccc ggccatcggc ggggatccac 300 cgattcgtgt tcatcgtgtt ccggcaggcg gtccggcagg Cgatctacgc gcctgggtgg 360 cgcgccaact tcaacaccag ggacttcgcc gcctgctaca gcctcggacc gcCtgtcgcc 420 gccacctact tcaactgcca gagggagggc ggctgcggtg gtcggaggta caggtgatga 480 atcgagagag agcatgcatc ccaacaaggc ggtgatgaca cgtgacccat cctatgacaa 540 gttatatata ttagcacata ccacaaaaat aaacaataca tatatatatg tctccatctc 600 tatctgcaat atn 6l3 <210> 54 <211> 158 <212> PRT
<213> Zea mays <400> 54 Tyr Phe Ser Ala Ser Ala Leu Leu Arg Val Met Tyr Gly Gly Arg Glu Met Thr Cys Gly Ser G1u Leu Arg Pro Ser Gln Val Ala Ser Glu Pro Thr Val His Ile Thr G1y Gly Arg Asp Gly Arg Pro Val Leu Tyr Thr Leu Val Met Leu Asp Pro Asp Ala Pro Ser Pro Ser Asn Pro Ser Lys Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Glu Gly Ala Gly A1a Asn His Gly Asn Glu Val Val Ala Tyr Glu Ser Pro Arg Pro Ser Ala Gly Ile His Arg Phe Val Phe Ile Val Phe Arg Gln Ala Val Arg Gln Ala Ile Tyr Ala Pro Gly Trp Arg Ala Asn Phe Asn Thr Arg Asp Phe Ala Ala Cys Tyr Ser Leu Gly Pro Pro Val Ala Ala Thr Tyr Phe l30 135 140 Asn Cys Gln Arg Glu Gly Gly Cys Gly Gly Arg Arg Tyr Arg <210> 55 <211> 945 <212> DNA
<213> Oryza sativa <400> 55 gcacgaggga gagatcgatg gcccgtttcg tggatccgct ggtggtggga cgggtgatcg 60 gggaggtggt ggatttgttc gttccatcca tctccatgac cgccgcctac ggcgacaggg 120 acatcagcaa cggctgcctc gtccgcccat ccgccgccga ctaccctccc ctcgtccgca 180 tctccggccg ccgcaacgac ctctacaccc tgatcatgac ggacccggac gcacctagcc 240 ctagcgaccc atccatgagg gagtttctcc actggatcgt ggttaacata ccggggggaa 300 cagatgcatc taaaggtgag gagatggtgg agtacatggg gccacggccg acggtgggga 360 tacacaggta cgtgctggtg ctgtacgagc agaaggcgcg cttcgtggac ggcgcgctga 420 tgccgccggc ggacaggccc aacttcaaca caagagcatt cgcggcgtac catcagctcg 480 gcctccccac cgccgtcgtc cacttcaact cccagaggga gcccgccaac cgccgccgct 540 aatagtaata gcctactatc tctatctatc tatccataat gaagaaagca agcacgcctg 600 cggatgcggc cggccggccc tactatatta ttacaataat atagtttttg aataattaag 660 ctagctagct ctcaactcaa gtatacttac tggaactcga ctgcgttgcg tacgcatgtc 720 ctcatcatac gtacgaacgt gcgtgtccac gtactgtgta ctagctagcg agtactctct 780 ccatatatat cttcctccac cgtcgtgtgg tacgttttaa caacgtacat gcatgcatgg 840 ataatgcagg ctctatatat atatatataa tactactgta ctgtactgta tgctttaatt 900 aattttgtgg tttgctctca aaaaaaaaaa aaaaaaaaaa aaaaa 945 <210> 56 <211> 174 <212> PRT
<213> Oryza sativa <400> 56 Met Ala Arg Phe Val Asp Pro Leu Val Val Gly Arg Va1 Ile Gly Glu Val Val Asp Leu Phe Val Pro Ser Ile Ser Met Thr Ala Ala Tyr Gly Asp Arg Asp I1e Ser Asn Gly Cys Leu Val Arg Pro Ser Ala Ala Asp Tyr Pro Pro Leu Val Arg Ile Ser Gly Arg Arg Asn Asp Leu Tyr Thr Leu Ile Met Thr Asp Pro Asp Ala Pro Ser Pro Ser Asp Pro Ser Met Arg Glu Phe Leu His Trp Ile Val Val Asn Ile Pro Gly Gly Thr Asp Ala Ser Lys Gly Glu Glu Met Val Glu Tyr Met Gly Pro Arg Pro Thr Val Gly Ile His Arg Tyr Val Leu Val Leu Tyr Glu Gln Lys Ala Arg Phe Val Asp Gly Ala Leu Met Pro Pro Ala Asp Arg Pro Asn Phe Asn Thr Arg Ala Phe Ala Ala Tyr His Gln Leu Gly Leu Pro Thr Ala Val Val His Phe Asn Ser Gln Arg Glu Pro Ala Asn Arg Arg Arg <210> 57 <211> 639 <212> DNA
<213> Oryza sativa <400> 57 gcacgagctt acacctaatc ccagcaaccc aaccttgagg gaatacctgc actggatggt 60 gactgatatc ccatcatcga cggacgatag ctttgggcgg gagatcgtaa catacgaaag 120 cccaagcccc accatgggca tccaccgcat cgtgatggtg ttgtatcagc agcttgggcg 180 cggcacggtg ttcgcgccgc aggtgcgtca gaacttcaac ctgcgcagct tcgcgcgccg 240 tttcaacctc ggcaagccgg tggccgccat gtacttcaac tgccagcgcc cgacaggcac 300 aggtgggagg aggccaacct gatctgatca atatcgatcg atcttcgatc ttctagctct 360 tgtacatgtt gagtgttgac caatataatg gccactcatg catatatata tatatgcagt 420 gtgtctagcc agctgcatgc aactttgtct acgtgcttat ataattaaac aaatgcatat 480 atagccggcc gtatcataaa gttcctagct ataaaagcta tagaataaat gtcgccccac 540 ttggtcagtt ggtgtacatg acggctccta agtgtgctat catgaatatg ctaataatag 600 cagtttagta tatcatcccc gcaaaaaaaa aaaaaaaaa 639 <210> 58 <211> 104 <212> PRT
<213> Oryza sativa <400> 58 Leu Thr Pro Asn Pro Ser Asn Pro Thr Leu Arg Glu Tyr Leu His Trp Met Val Thr Asp Ile Pro Ser Ser Thr Asp Asp Ser Phe Gly Arg Glu Ile Val Thr Tyr Glu Ser Pro Ser Pro Thr Met Gly Ile His Arg Ile Val Met Val Leu Tyr Gln Gln Leu G1y Arg Gly Thr Val Phe Ala Pro Gln Val Arg Gln Asn Phe Asn Leu Arg Ser Phe Ala Arg Arg Phe Asn Leu Gly Lys Pro Val Ala Ala Met Tyr Phe Asn Cys G1n Arg Pro Thr Gly Thr Gly Gly Arg Arg Pro Thr <210> 59 <211> 1004 <212> DNA
<213> Oryza sativa <400> 59 gcacgagctc aagttagctt cttagcacag cctcttcttg ctcaactcct gaagatcatc 60 aatcttcact agccatgtca agggacccac ttgtcgtagg acatgttgtt ggggatatct 120 tagacccatt caacaaatca gcatcactca aggtcctata caacaacaag gaattaacaa 180 atgggtctga gctcaaaccg tcacaggtag caaatgaacc aaggattgaa attgctggcc 240 gcgacataag gaacctttac actctggtga tggtggatcc tgactcgcca agtccaagca 300 acccaacaaa aagagaatac cttcattggt tggtgacaga cattccagaa tcggcaaatg 360 ctagttatgg aaatgaagtt gtcagttatg aaagcccaaa accaactgca gggatacatc 420 gttttgtctt tatattattt cgccaatatg tacaacagac tatttatgca ccaggatgga 480 gaccaaattt caatacaaga gatttttccg cactgtataa tcttggacct cctgtggcag 540 cagtgttctt caattgccag agggagaacg gatgtggagg cagacggtac attagataaa 600 agtcaggatc attcatagcc ctctacaaga agaggtgata ttcatgtgag aagatgaatg 660 gggtcaggca catcgcaacg tgctggtcaa tggtggacct tttaatgtat cttcatttaa 720 gaactactac ctttgatacg tatccaggca ctaaacaagg tgctttacga atgaatttag 780 cttcagatct catcttggag aacactttat ctggttcttc aggaacgaaa tcctactgat 840 tctgcaccca acaactgttg tccatgtcat gttcaaaagc gactatcaaa gcaacaaatt 900 gagtgcatca ttgaagaatg caactgataa cacgtcatgt tctttaaaaa agaaagcatc 960 ctaggcttac tgagaacttt gcataaaaaa aaaaaaaaaa aaaa 1004 <210> 60 <211> 174 <212> PRT
<213> Oryza sativa <400> 60 Met Ser Arg Asp Pro Leu Val Val Gly His Val Va1 Gly Asp Ile Leu Asp Pro Phe Asn Lys Ser Ala Ser Leu Lys Val Leu Tyr Asn Asn Lys Glu Leu Thr Asn G1y Ser Glu Leu Lys Pro Ser Gln Val Ala Asn Glu Pro Arg Ile Glu Ile Ala Gly Arg Asp Ile Arg Asn Leu Tyr Thr Leu Val Met Val Asp Pro Asp Ser Pro Ser Pro Ser Asn Pro Thr Lys Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Glu Ser Ala Asn Ala Ser Tyr Gly Asn Glu Val Val Ser Tyr Glu Ser Pro Lys Pro Thr Ala Gly Ile His Arg Phe Val Phe Ile Leu Phe Arg Gln Tyr Val Gln Gln Thr Ile Tyr Ala Pro Gly Trp Arg Pro Asn Phe Asn Thr Arg Asp Phe Ser Ala Leu Tyr Asn Leu Gly Pro Pro Val Ala Ala Val Phe Phe Asn Cys Gln Arg Glu Asn Gly Cys Gly Gly Arg Arg Tyr Ile Arg <210> 61 <2l1> 175 <212> PRT
<213> Arabidopsis thaliana <400> 61 Met Ser Ile Asn Ile Arg Asp Pro Leu I1e Val Ser Arg Val Val Gly Asp Val Leu Asp Pro Phe Asn Arg Ser Ile Thr Leu Lys Val Thr Tyr Gly Gln Arg Glu Va1 Thr Asn Gly Leu Asp Leu Arg Pro Ser Gln Val Gln Asn Lys Pro Arg Val Glu Ile Gly Gly Glu Asp Leu Arg Asn Phe Tyr Thr Leu Val Met Val Asp Pro Asp Val Pro Ser Pro Ser Asn Pro His Leu Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Ala Thr 85 . 90 95 Thr Gly Thr Thr Phe G1y Asn Glu Ile Val Cys Tyr Glu Asn Pro Ser Pro Thr Ala Gly Ile His Arg Val Val Phe Ile Leu Phe Arg Gln Leu Gly Arg Gln Thr Val Tyr Ala Pro Gly Trp Arg G1n Asn Phe Asn Thr Arg Glu Phe Ala Glu Ile Tyr Asn Leu Gly Leu Pro Val Ala Ala Val Phe Tyr Asn Cys Gln Arg Glu Ser Gly Cys Gly Gly Arg Arg Leu <210> 62 <211> 179 <212> PRT
<213> Oryza sativa <400> 62 Met Ala Gly Ser Gly Arg Asp Arg Asp Pro Leu Val Val Gly Arg Val l 5 10 15 Val Gly Asp Val Leu Asp Ala Phe Val Arg Ser Thr Asn Leu Lys Val Thr Tyr Gly Ser Lys Thr Val Ser Asn Gly Cys Glu Leu Lys Pro Ser Met Val Thr His Gln Pro Arg Val Glu Val Gly Gly Asn Asp Met Arg Thr Phe Tyr Thr Leu Val Met Val Asp Pro Asp Ala Pro Ser Pro Ser Asp Pro Asn Leu Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Gly Thr Thr Ala Ala Ser Phe Gly Gln Glu Val Met Cys Tyr Glu Ser Pro Arg Pro Thr Met Gly Ile His Arg Leu Val Phe Val Leu Phe Gln Gln Leu G1y Arg Gln Thr Val Tyr Ala Pro Gly Trp Arg Gln Asn Phe Asn Thr Lys Asp Phe Ala Glu Leu Tyr Asn Leu Gly Ser Pro Val Ala Ala Val Tyr Phe Asn Cys G1n Arg Glu Ala Gly Ser Gly Gly Arg Arg Val Tyr Pro

Claims (20)

What is claimed is:
1. An isolated polynucleotide comprising:
(a) a first nucleotide sequence encoding a polypeptide having FT
homolog activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:4, 6, 8, 10, 12, 14, 16, 18, 26, 28, 30, 34, 36, 40, 42, 44, 54, 56, 58, or 60, have at least 80% sequence identity based on the Clustal alignment method, or (b) a second nucleotide sequence encoding a polypeptide having FT
homolog activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 22, 24, 32, or 38, have at least 85% sequence identity based on the Clustal alignment method, or (c) a third nucleotide sequence encoding a polypeptide having Ap3 homolog activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:50,has at least 90% sequence identity based on the Clustal alignment method, or (d) a fourth nucleotide sequence encoding a polypeptide having FT
homolog activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:20, has at least 95% sequence identity based on the Clustal alignment method, or (e) a fifth nucleotide sequence encoding a polypeptide having Ap3 homolog activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:46, or 48, have at least 95% sequence identity based on the Clustal alignment method, or (f) the complement of the first, second, third, fourth, or fifth, nucleotide sequence, wherein the complement and the nucleotide sequence contain the same number of nucleotides and are 100%
complementary.
2. The polynucleotide of Claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:4, 6, 8, 10, 12, 14, 16, 18, 26, 28, 30, 34, 36, 40, 42, 44, 54, 56, 58, or 60, have at least 85% identity based on the Clustal alignment method.
3. The polynucleotide of Claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 54, 56, 58, or 60, have at least 90% identity based on the Clustal alignment method.
4. The polynucleotide of Claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 50, 54, 56, 58, or 60 have at least 95% identity based on the Clustal alignment method.
5. The polynucleotide of Claim 1, wherein the amino acid sequence of the polypeptide comprises the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 54, 56, 58, or 60.
6. The polynucleotide of Claim 1 wherein the nucleotide sequence comprises the nucleotide sequence of SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 37, 39, 41, 43, 45, 47, 49, 53, 55, 57, or 59.
7. A vector comprising the polynucleotide of Claim 1.
8. A recombinant DNA construct comprising the polynucleotide of Claim 1 operably linked to a regulatory sequence.
9. A method for transforming a cell, comprising transforming a cell with the polynucleotide of Claim 1.
10. A cell comprising the recombinant DNA construct of Claim 8.
11. A method for producing a plant comprising transforming a plant cell with the polynucleotide of Claim 1 and regenerating a plant from the transformed plant cell.
12. A plant comprising the recombinant DNA construct of Claim 8.
13. A seed comprising the recombinant DNA construct of Claim 8.
14. An isolated polynucleotide comprising a first nucleotide sequence, wherein the first nucleotide sequence contains at least 30 nucleotides, and wherein the first nucleotide sequence is comprised by another polynucleotide, wherein the other polynucleotide includes:
(a) a second nucleotide sequence, wherein the second nucleotide sequence encodes a polypeptide having FT homolog activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:4, 6, 8, 10, 12, 14, 16, 18, 26, 28, 30, 34, 36, 40, 42, 44, 54, 56, 58, or 60, having at least 80% sequence identity based on the Clustal alignment method, or (b) a third nucleotide sequence, wherein the third nucleotide sequence encodes a polypeptide having FT homolog activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 22, 24, 32, or 38, having at least 85%
sequence identity based on the Clustal alignment method, or (c) a fourth nucleotide sequence, wherein the fourth nucleotide sequence encodes a polypeptide having Ap3 homolog activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:50 has at least 90% sequence identity based on the Clustal alignment method, or (d) a fifth nucleotide sequence, wherein the fifth nucleotide sequence encodes a polypeptide having FT homolog activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:20 has at least 95% sequence identity based on the Clustal alignment method, or (e) a sixth nucleotide sequence, wherein the sixth nucleotide sequence encodes a polypeptide having Ap3 homolog activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:46, or 48, has at least 95% sequence identity based on the Clustal alignment method, or (f) the complement of the second, third, fourth, fifth, or sixth nucleotide sequence, wherein the complement and the second, third, fourth, fifth, or sixth nucleotide sequence contain the came number of nucleotides and are 100% complementary.
15. An isolated polypeptide having FT or Ap3 homolog activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID
NO:4, 6, 8, 10, 12, 14, 16, 18, 26, 28, 30, 34, 36, 40, 42, 44, 54, 56, 58, or 60, have at least 80% identity based on the Clustal alignment method.
16. The polypeptide of Claim 15, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 54, 56, 58, or 60, have at least 85% identity based on the Clustal alignment method.
17. The polypeptide of Claim 15, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 50, 54, 56, 58, or 60 have at least 90% identity based on the Clustal alignment method.
18. The polypeptide of Claim 15, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 54, 56, 58, or 60 have at least 95% identity based on the Clustal alignment method.
19. The polypeptide of Claim 15, wherein the amino acid sequence of the polypeptide comprises the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 54, 56, 58, or 60.
20. A method for isolating a polypeptide encoded by the polynucleotide of Claim 1 comprising isolating the polypeptide from a cell containing a recombinant DNA construct comprising the polynucleotide operably linked to a regulatory sequence.
CA002436778A 2000-11-28 2001-11-21 Floral development genes Abandoned CA2436778A1 (en)

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US20050066394A1 (en) * 2000-11-28 2005-03-24 Olga Danilevskaya Floral development genes
JP2004016201A (en) 2002-06-20 2004-01-22 National Agriculture & Bio-Oriented Research Organization Flower bud formation suppressor gene and plant provided with early flowering property
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