CA2428248A1 - Constructions de genes rapporteurs de solubilite - Google Patents

Constructions de genes rapporteurs de solubilite Download PDF

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Publication number
CA2428248A1
CA2428248A1 CA002428248A CA2428248A CA2428248A1 CA 2428248 A1 CA2428248 A1 CA 2428248A1 CA 002428248 A CA002428248 A CA 002428248A CA 2428248 A CA2428248 A CA 2428248A CA 2428248 A1 CA2428248 A1 CA 2428248A1
Authority
CA
Canada
Prior art keywords
cell
solubility
protein
host cell
target polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002428248A
Other languages
English (en)
Inventor
Scott Lesley
Mark Knuth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IRM LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2428248A1 publication Critical patent/CA2428248A1/fr
Abandoned legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245Escherichia (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA002428248A 2000-11-21 2001-11-21 Constructions de genes rapporteurs de solubilite Abandoned CA2428248A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US72134000A 2000-11-21 2000-11-21
US09/721,340 2000-11-21
US32483301P 2001-09-24 2001-09-24
US60/324,833 2001-09-24
PCT/US2001/051426 WO2002061041A2 (fr) 2000-11-21 2001-11-21 Constructions de genes rapporteurs de solubilite

Publications (1)

Publication Number Publication Date
CA2428248A1 true CA2428248A1 (fr) 2002-08-08

Family

ID=26984650

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002428248A Abandoned CA2428248A1 (fr) 2000-11-21 2001-11-21 Constructions de genes rapporteurs de solubilite

Country Status (4)

Country Link
EP (1) EP1364055A4 (fr)
JP (1) JP2004535772A (fr)
CA (1) CA2428248A1 (fr)
WO (1) WO2002061041A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016062819A1 (fr) * 2014-10-22 2016-04-28 Danmarks Tekniske Universitet Système rapporteur à deux cassette destiné à évaluer la traduction de gène cible et la formation de corps d'inclusion de produit de gène cible
CN109055417B (zh) * 2018-08-28 2020-07-07 浙江新和成股份有限公司 一种重组微生物、其制备方法及其在生产辅酶q10中的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5589337A (en) * 1992-07-06 1996-12-31 The President And Fellows Of Harvard College Methods and diagnostic kits for determining toxicity utilizing bacterial stress promoters fused to reporter genes
CA2200702C (fr) * 1994-11-23 2000-12-05 E.I. Du Pont De Nemours And Company Reactif bacterien bioluminescent lyophilise servant a la detection d'agents toxiques

Also Published As

Publication number Publication date
WO2002061041A2 (fr) 2002-08-08
EP1364055A2 (fr) 2003-11-26
WO2002061041A9 (fr) 2004-05-13
JP2004535772A (ja) 2004-12-02
WO2002061041A3 (fr) 2003-09-04
EP1364055A4 (fr) 2004-08-25

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Legal Events

Date Code Title Description
FZDE Discontinued