EP1364055A4 - Constructions de genes rapporteurs de solubilite - Google Patents

Constructions de genes rapporteurs de solubilite

Info

Publication number
EP1364055A4
EP1364055A4 EP01998134A EP01998134A EP1364055A4 EP 1364055 A4 EP1364055 A4 EP 1364055A4 EP 01998134 A EP01998134 A EP 01998134A EP 01998134 A EP01998134 A EP 01998134A EP 1364055 A4 EP1364055 A4 EP 1364055A4
Authority
EP
European Patent Office
Prior art keywords
reporter gene
gene constructs
solubility reporter
solubility
constructs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP01998134A
Other languages
German (de)
English (en)
Other versions
EP1364055A2 (fr
Inventor
Scott Lesley
Mark Knuth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IRM LLC
Original Assignee
IRM LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IRM LLC filed Critical IRM LLC
Publication of EP1364055A2 publication Critical patent/EP1364055A2/fr
Publication of EP1364055A4 publication Critical patent/EP1364055A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP01998134A 2000-11-21 2001-11-21 Constructions de genes rapporteurs de solubilite Withdrawn EP1364055A4 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US72134000A 2000-11-21 2000-11-21
US721340 2000-11-21
US32483301P 2001-09-24 2001-09-24
US324833P 2001-09-24
PCT/US2001/051426 WO2002061041A2 (fr) 2000-11-21 2001-11-21 Constructions de genes rapporteurs de solubilite

Publications (2)

Publication Number Publication Date
EP1364055A2 EP1364055A2 (fr) 2003-11-26
EP1364055A4 true EP1364055A4 (fr) 2004-08-25

Family

ID=26984650

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01998134A Withdrawn EP1364055A4 (fr) 2000-11-21 2001-11-21 Constructions de genes rapporteurs de solubilite

Country Status (4)

Country Link
EP (1) EP1364055A4 (fr)
JP (1) JP2004535772A (fr)
CA (1) CA2428248A1 (fr)
WO (1) WO2002061041A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3209795B1 (fr) * 2014-10-22 2019-10-16 Danmarks Tekniske Universitet Système rapporteur deux-cassettes pour évaluer la translation du gène cible, produit du gène cible et la formation de corps d'inclusion
CN109055417B (zh) * 2018-08-28 2020-07-07 浙江新和成股份有限公司 一种重组微生物、其制备方法及其在生产辅酶q10中的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0651825B1 (fr) * 1992-07-06 1998-01-14 President And Fellows Of Harvard College Procedes et necessaires de diagnostic pour determiner la toxicite d'une composition utilisant des promoteurs de stress bacteriens fusionnes a des genes rapporteurs
CA2200702C (fr) * 1994-11-23 2000-12-05 E.I. Du Pont De Nemours And Company Reactif bacterien bioluminescent lyophilise servant a la detection d'agents toxiques

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HOFFMANN FRANK ET AL: "Kinetics of heat-shock response and inclusion body formation during temperature-induced production of basic fibroblast growth factor in high-cell-density cultures of recombinant Escherichia coli", BIOTECHNOLOGY PROGRESS, vol. 16, no. 6, November 2000 (2000-11-01), pages 1000 - 1007, XP002282860, ISSN: 8756-7938 *
KANEMORI MASAAKI ET AL: "Induction of heat shock proteins by abnormal proteins results from stabilization and not increased synthesis of sigma-32 in Escherichia coli", JOURNAL OF BACTERIOLOGY, vol. 176, no. 18, 1994, pages 5648 - 5653, XP002282859, ISSN: 0021-9193 *
PARSELL D A ET AL: "PROTEIN DISAGGREGATION MEDIATED BY HEAT-SHOCK PROTEIN HSP104", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 372, 1 December 1994 (1994-12-01), pages 475 - 478, XP008023071, ISSN: 0028-0836 *
See also references of WO02061041A3 *
WEINER LORIN ET AL: "Analysis of the proteins and cis-acting elements regulating the stress-induced phage shock protein operon", NUCLEIC ACIDS RESEARCH, vol. 23, no. 11, 1995, pages 2030 - 2036, XP002282861, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
EP1364055A2 (fr) 2003-11-26
CA2428248A1 (fr) 2002-08-08
WO2002061041A9 (fr) 2004-05-13
JP2004535772A (ja) 2004-12-02
WO2002061041A3 (fr) 2003-09-04
WO2002061041A2 (fr) 2002-08-08

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Legal Events

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Effective date: 20040708

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Effective date: 20060607