CA2419975A1 - Enzymes having high temperature polymerase activity and methods of use thereof - Google Patents

Enzymes having high temperature polymerase activity and methods of use thereof Download PDF

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Publication number
CA2419975A1
CA2419975A1 CA002419975A CA2419975A CA2419975A1 CA 2419975 A1 CA2419975 A1 CA 2419975A1 CA 002419975 A CA002419975 A CA 002419975A CA 2419975 A CA2419975 A CA 2419975A CA 2419975 A1 CA2419975 A1 CA 2419975A1
Authority
CA
Canada
Prior art keywords
nucleic acid
sequence
polypeptide
leu
glu
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002419975A
Other languages
English (en)
French (fr)
Inventor
Walter Callen
Eric J. Mathur
Jay M. Short
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2419975A1 publication Critical patent/CA2419975A1/en
Abandoned legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/05Animals comprising random inserted nucleic acids (transgenic)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA002419975A 2000-09-06 2001-09-06 Enzymes having high temperature polymerase activity and methods of use thereof Abandoned CA2419975A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/656,309 US7049101B1 (en) 1997-08-06 2000-09-06 Enzymes having high temperature polymerase activity and methods of use thereof
US09/656,309 2000-09-06
PCT/US2001/028007 WO2002020735A2 (en) 2000-09-06 2001-09-06 Enzymes having high temperature polymerase activity and methods of use thereof

Publications (1)

Publication Number Publication Date
CA2419975A1 true CA2419975A1 (en) 2002-03-14

Family

ID=24632506

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002419975A Abandoned CA2419975A1 (en) 2000-09-06 2001-09-06 Enzymes having high temperature polymerase activity and methods of use thereof

Country Status (6)

Country Link
US (5) US7049101B1 (enExample)
EP (1) EP1315803A2 (enExample)
JP (1) JP2004523211A (enExample)
AU (1) AU2001288859A1 (enExample)
CA (1) CA2419975A1 (enExample)
WO (1) WO2002020735A2 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPS115502A0 (en) * 2002-03-18 2002-04-18 Diatech Pty Ltd Assessing data sets
BR0314558A (pt) * 2002-10-10 2005-08-09 Diversa Corp Proteases, ácidos nucléicos codificando as mesmas e processos para preparar e usar as mesmas
US7960157B2 (en) * 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
DK2853593T3 (en) 2003-03-07 2018-01-08 Dsm Ip Assets Bv Hydrolases, nucleic acids encoding them, and processes for their preparation and use
US20080070291A1 (en) 2004-06-16 2008-03-20 David Lam Compositions and Methods for Enzymatic Decolorization of Chlorophyll
WO2006031699A2 (en) 2004-09-10 2006-03-23 Diversa Corporation Compositions and methods for making and modifying oils
WO2006030455A1 (en) * 2004-09-17 2006-03-23 Prokaria Ehf. Dna polymerases having strand displacement activity
WO2009135158A2 (en) * 2008-05-02 2009-11-05 Immunetics, Inc. Detection of microbial nucleic acids
GB201007384D0 (en) 2010-04-30 2010-06-16 Medical Res Council Enzymes
CN103898077B (zh) 2012-12-24 2017-01-11 财团法人工业技术研究院 单离的脱氧核糖核酸聚合酶、套组及其应用
US10494665B2 (en) * 2014-08-20 2019-12-03 Huawei Yang Test kit and method for testing target nucleic acid in sample
CN111684064A (zh) 2018-01-19 2020-09-18 生物辐射实验室股份有限公司 突变dna聚合酶

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1531A (en) * 1840-03-31 Kockiltor-chaib
US4165188A (en) 1977-02-17 1979-08-21 Sycor, Inc. Ribbon mask and guide for dot matrix impact printers
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
IL95738A (en) * 1989-09-23 1996-11-14 Hoechst Ag Antibodies against the well-preserved amino acid sequences of insulin, a process for their preparation and use in immunoassay
US5322785A (en) 1990-04-26 1994-06-21 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
US5770429A (en) * 1990-08-29 1998-06-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5541311A (en) 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5491086A (en) * 1993-05-14 1996-02-13 Hoffmann-La Roche Inc. Purified thermostable nucleic acid polymerase and DNA coding sequences from pyrodictium species
USH1531H (en) 1994-04-18 1996-05-07 Blumentals; Ilse I. Thermophilic DNA polymerase
JP3132624B2 (ja) 1994-05-09 2001-02-05 東洋紡績株式会社 超好熱始原菌由来のdnaポリメラーゼ遺伝子およびその用途
JP3498808B2 (ja) 1994-06-09 2004-02-23 タカラバイオ株式会社 Dnaポリメラーゼ遺伝子
US6077664A (en) 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US5939250A (en) * 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
DE69725076T2 (de) 1996-07-29 2004-04-15 Toyo Boseki K.K. Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
US6057098A (en) * 1997-04-04 2000-05-02 Biosite Diagnostics, Inc. Polyvalent display libraries
US5948666A (en) 1997-08-06 1999-09-07 Diversa Corporation Isolation and identification of polymerases

Also Published As

Publication number Publication date
US8148126B2 (en) 2012-04-03
US20020115108A1 (en) 2002-08-22
US7049101B1 (en) 2006-05-23
JP2004523211A (ja) 2004-08-05
EP1315803A2 (en) 2003-06-04
US20020132997A1 (en) 2002-09-19
US20020164618A1 (en) 2002-11-07
US20110195479A1 (en) 2011-08-11
AU2001288859A1 (en) 2002-03-22
US7781198B2 (en) 2010-08-24
WO2002020735A3 (en) 2003-03-06
WO2002020735A2 (en) 2002-03-14

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued