CA2411515A1 - Generation et criblage a haut rendement d'un repertoire d'anticorps entierement humains dans la levure - Google Patents
Generation et criblage a haut rendement d'un repertoire d'anticorps entierement humains dans la levure Download PDFInfo
- Publication number
- CA2411515A1 CA2411515A1 CA002411515A CA2411515A CA2411515A1 CA 2411515 A1 CA2411515 A1 CA 2411515A1 CA 002411515 A CA002411515 A CA 002411515A CA 2411515 A CA2411515 A CA 2411515A CA 2411515 A1 CA2411515 A1 CA 2411515A1
- Authority
- CA
- Canada
- Prior art keywords
- library
- tester
- target
- yeast
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Compositions, kits et procédés pour générer des bibliothèques hautement diversifiées de protéines telles que les anticorps par le biais de la recombinaison homologue in vivo ainsi que criblage de ces bibliothèques à la recherche de cibles de protéines, peptides et acides nucléiques, et ce au moyen d'un procédé à deux hybrides dans la levure. Le procédé destiné au criblage d'une bibliothèque des protéines de fusion de test à la recherche d'une protéine ou peptide cible comprend ce qui suit: exprimer une bibliothèque de protéines de test dans les cellules de levure, la protéine de fusion de test comprenant une première sous-unité polypeptidique dont la séquence varie à l'intérieur de la bibliothèque indépendamment du premier polypeptide ainsi qu'un peptide de liaison qui relie la première et la deuxième sous-unités de polypeptides; exprimer une ou plusieurs protéines de fusion cibles dans les cellules de la levure exprimant les protéines de test, chacune des protéines de fusion cibles comprenant une protéine ou un peptide cible; et sélectionner celles des cellules de levure dans lesquelles un gène rapporteur est exprimé, l'expression du gène rapporteur étant activée par la liaison de la protéine de fusion de test à la protéine de fusion cible.
Applications Claiming Priority (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US60297200A | 2000-06-23 | 2000-06-23 | |
US09/603,663 | 2000-06-23 | ||
US09/602,972 | 2000-06-23 | ||
US09/603,658 | 2000-06-23 | ||
US09/603,663 US6406863B1 (en) | 2000-06-23 | 2000-06-23 | High throughput generation and screening of fully human antibody repertoire in yeast |
US09/602,373 US6410271B1 (en) | 2000-06-23 | 2000-06-23 | Generation of highly diverse library of expression vectors via homologous recombination in yeast |
US09/603,658 US6410246B1 (en) | 2000-06-23 | 2000-06-23 | Highly diverse library of yeast expression vectors |
US09/602,373 | 2000-06-23 | ||
PCT/US2001/020542 WO2002000729A2 (fr) | 2000-06-23 | 2001-06-25 | Generation et criblage a haut rendement d'un repertoire d'anticorps entierement humains dans la levure |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2411515A1 true CA2411515A1 (fr) | 2002-01-03 |
Family
ID=27505059
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002411515A Abandoned CA2411515A1 (fr) | 2000-06-23 | 2001-06-25 | Generation et criblage a haut rendement d'un repertoire d'anticorps entierement humains dans la levure |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1297124A2 (fr) |
CN (1) | CN1444651A (fr) |
AU (1) | AU2001271553A1 (fr) |
CA (1) | CA2411515A1 (fr) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101348526B (zh) * | 2008-08-29 | 2011-08-17 | 浙江大学 | 一种抗白细胞介素-8抗体 |
CN104342449A (zh) * | 2013-08-07 | 2015-02-11 | 太仓美诺恒康生物技术有限公司 | 抗体表达载体的构建方法 |
CN104725501A (zh) * | 2013-12-20 | 2015-06-24 | 深圳先进技术研究院 | 构建hiv病毒抗体酵母展示库的方法和筛选病毒广谱中和性抗体的方法及其应用 |
CN103910799B (zh) * | 2014-03-06 | 2016-03-23 | 中国人民解放军第四军医大学 | 一种基于抗egfr单链抗体及精氨酸九聚体的融合蛋白与应用 |
CN108728476A (zh) * | 2017-04-14 | 2018-11-02 | 复旦大学 | 一种利用crispr系统产生多样性抗体文库的方法 |
AU2019308317A1 (en) * | 2018-07-19 | 2021-01-28 | University Of Washington | De novo design of protein switches |
SG11202102700TA (en) * | 2018-11-30 | 2021-04-29 | Illumina Inc | Analysis of multiple analytes using a single assay |
KR102683424B1 (ko) * | 2019-03-18 | 2024-07-10 | 리제너론 파마슈티칼스 인코포레이티드 | 타우 응집과 관련된 유전적 취약성을 식별하기 위한 CRISPR/Cas 드랍아웃 스크리닝 플랫폼 |
CN111724858B (zh) * | 2020-05-14 | 2024-06-07 | 东北林业大学 | 利用软件运行基因组序列比对修补gap的方法 |
CN112175984A (zh) * | 2020-09-18 | 2021-01-05 | 中国科学院深圳先进技术研究院 | 基于合成基因和酿酒酵母同源重组机制的分子克隆方法 |
-
2001
- 2001-06-25 CA CA002411515A patent/CA2411515A1/fr not_active Abandoned
- 2001-06-25 CN CN 01813639 patent/CN1444651A/zh active Pending
- 2001-06-25 AU AU2001271553A patent/AU2001271553A1/en not_active Abandoned
- 2001-06-25 EP EP01950579A patent/EP1297124A2/fr not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
AU2001271553A1 (en) | 2002-01-08 |
CN1444651A (zh) | 2003-09-24 |
EP1297124A2 (fr) | 2003-04-02 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Discontinued |