CA2407933A1 - A method of separating viable cells from cell suspensions - Google Patents

A method of separating viable cells from cell suspensions Download PDF

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Publication number
CA2407933A1
CA2407933A1 CA002407933A CA2407933A CA2407933A1 CA 2407933 A1 CA2407933 A1 CA 2407933A1 CA 002407933 A CA002407933 A CA 002407933A CA 2407933 A CA2407933 A CA 2407933A CA 2407933 A1 CA2407933 A1 CA 2407933A1
Authority
CA
Canada
Prior art keywords
diameter
outlet device
hydrocyclone
run
top run
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002407933A
Other languages
French (fr)
Other versions
CA2407933C (en
Inventor
Wolf-Dieter Deckwer
Birger Anspach
Ricardo De Andrade Medronho
Marc Luebberstedt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2407933A1 publication Critical patent/CA2407933A1/en
Application granted granted Critical
Publication of CA2407933C publication Critical patent/CA2407933C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a method for separating viable cells from cell suspensions using hydrocyclones. In particular, said method can be carried out continuously.

Claims (5)

1. Method of separating viable mammalian or insect cells from cell suspensions, wherein the cell suspension is introduced, by way of an inlet device, tangentially into the cylinder space of at least one hydrocyclone and is allowed to flow in a downwardly directed, spirally circling course through the cone of the hydrocyclone, a suspension of increased cell concentration being collected at the bottom run-off outlet device of the hydrocyclone, located at the bottom end of the cone, and a suspension of decreased cell concentration being collected at the top run-off outlet device, which is arranged centrally in, and extends into, the cylinder space, and the pressure drop between the inlet device and the top run-off outlet device being at most 4 bar, the hydrocyclone having the following features of construction:

cylindrical portion:
length 2 to 12 mm diameter 5 to 15 mm conical portion:
angle of taper 5 to 20°
inlet device:
diameter 0.5 to 4 mm top run-off outlet device:
diameter 1 to 5 mm depth of introduction 1 to 6 mm bottom run-off outlet device:
diameter 0.5 to 7 mm.
2. Method according to claim 1, wherein the pressure drop between the inlet device and the top run-off outlet device is 2 bar.
3. Method according to claim 2, wherein the hydrocyclone has the following features of construction:

cylindrical portion:
length 2 to 12 mm diameter 10 mm conical portion:

angle of taper 5 to 20°
inlet device:
diameter 2 mm top run-off outlet device:
diameter 1 to 5 mm depth of introduction 1 to 6 mm bottom run-off outlet device:
diameter 0.5 to 7 mm.
4. Method according to one of the preceding claims, wherein a plurality of identical or different hydrocyclones arranged in parallel or in series are operated discontinuously or continuously.
5. Method according to one of the preceding claims, wherein the mammalian cells are blood cells or hybridoma cells.
CA2407933A 2000-05-11 2001-03-30 A method of separating viable cells from cell suspensions Expired - Fee Related CA2407933C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10022635.3 2000-05-11
DE10022635A DE10022635A1 (en) 2000-05-11 2000-05-11 Separation of viable biological cells from their suspensions, comprises a process takes place in robust hydrocyclone with controlled maximum pressure
PCT/EP2001/003665 WO2001085902A1 (en) 2000-05-11 2001-03-30 Method for separating viable cells from cell suspensions

Publications (2)

Publication Number Publication Date
CA2407933A1 true CA2407933A1 (en) 2001-11-15
CA2407933C CA2407933C (en) 2010-07-20

Family

ID=7641365

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2407933A Expired - Fee Related CA2407933C (en) 2000-05-11 2001-03-30 A method of separating viable cells from cell suspensions

Country Status (8)

Country Link
US (1) US6878545B2 (en)
EP (1) EP1280885B1 (en)
AT (1) ATE307193T1 (en)
CA (1) CA2407933C (en)
DE (2) DE10022635A1 (en)
DK (1) DK1280885T3 (en)
ES (1) ES2250393T3 (en)
WO (1) WO2001085902A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7488448B2 (en) * 2004-03-01 2009-02-10 Indian Wells Medical, Inc. Method and apparatus for removal of gas bubbles from blood
CA2524956C (en) * 2003-05-09 2013-09-24 Gbf-Gesellschaft Fur Biotechnologische Forschung Mbh Method, apparatus and system for separating eucaryotic or procaryotic cells or other biological material from a suspension
DE102007005369A1 (en) * 2007-02-02 2008-08-07 Eppendorf Ag Device and method for the mechanical separation of cells from a cell network
WO2009023562A2 (en) 2007-08-09 2009-02-19 Wyeth Use of perfusion to enhance production of fed-batch cell culture in bioreactors
DE102008029836B4 (en) 2008-06-25 2010-12-02 Sartorius Stedim Biotech Gmbh Apparatus and method for the continuous removal of low-particulate solution from a bioreactor
DE102010042645A1 (en) * 2010-10-19 2012-04-19 Dürr Systems GmbH Plant for the transfer of heat or from cold to fluid medium
CN104780954B (en) 2012-08-15 2017-05-03 旋风医疗科技股份有限公司 Systems and methods for salvaging red blood cells for autotransfusion
JP6328229B2 (en) * 2014-03-31 2018-05-23 ホソカワミクロン株式会社 Classifier
GB201904083D0 (en) 2019-03-25 2019-05-08 Ge Healthcare Bio Sciences Ab A bioreactor system
GB2585055B (en) * 2019-06-26 2022-06-01 Cell Therapy Catapult Ltd Separating system
GB2602128B (en) * 2020-12-18 2023-01-04 Cell Therapy Catapult Ltd Separating system
US20230242864A1 (en) * 2022-01-31 2023-08-03 Sciperio, Inc In-time storage of manufactured cells for medical cellular therapy grown and differentiated in bioreactors

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US453105A (en) * 1891-05-26 bretney
US3647633A (en) * 1967-11-22 1972-03-07 Canadian Patents Dev Apparatus for the continuous phased culturing of cells
DE2706831A1 (en) * 1977-02-17 1978-08-31 Eckes Fa Peter PROCEDURE FOR THE CONTINUOUS AGING OF AGEATABLE SOLUTIONS WITH YEAST
NL8102856A (en) * 1981-06-15 1983-01-03 Unilever Nv PROCESS FOR PREPARING BLOOD CELL PROTEIN AND HAEM FROM HAEMOGLOBINE.
DK164252C (en) * 1986-11-11 1995-10-30 Slagteriernes Forskningsinst Process for producing protein products from bone-containing animal material
SU1637882A1 (en) 1988-12-19 1991-03-30 Московский Институт Химического Машиностроения Hydrocyclone
JP2958789B2 (en) * 1990-01-22 1999-10-06 味の素株式会社 Method for purifying amino acids / nucleic acids and derivatives thereof
GB9015578D0 (en) 1990-07-16 1990-09-05 Pirtferm Ltd Fermentorbioreactor module
BE1010407A4 (en) 1996-07-04 1998-07-07 Undatim Ultrasonics Method and installation of water treatment.
US6146525A (en) * 1998-02-09 2000-11-14 Cycteck Environmental, Inc. Apparatus and methods for separating particulates from a particulate suspension in wastewater processing and cleaning
EP1359799A2 (en) * 2000-11-08 2003-11-12 Novus International, Inc. Methods and compositions for the control of coccidiosis

Also Published As

Publication number Publication date
CA2407933C (en) 2010-07-20
ES2250393T3 (en) 2006-04-16
ATE307193T1 (en) 2005-11-15
EP1280885B1 (en) 2005-10-19
US6878545B2 (en) 2005-04-12
DE50107753D1 (en) 2006-03-02
DK1280885T3 (en) 2006-01-30
US20030166269A1 (en) 2003-09-04
DE10022635A1 (en) 2001-11-15
WO2001085902A1 (en) 2001-11-15
EP1280885A1 (en) 2003-02-05

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Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20160330