CA2407346C - Blood components separator disk - Google Patents

Blood components separator disk Download PDF

Info

Publication number
CA2407346C
CA2407346C CA002407346A CA2407346A CA2407346C CA 2407346 C CA2407346 C CA 2407346C CA 002407346 A CA002407346 A CA 002407346A CA 2407346 A CA2407346 A CA 2407346A CA 2407346 C CA2407346 C CA 2407346C
Authority
CA
Canada
Prior art keywords
disk
tube
physiological fluid
component
combination according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002407346A
Other languages
French (fr)
Other versions
CA2407346A1 (en
Inventor
James R. Ellsworth
Steven F. Levesque
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvest Technologies Corp
Original Assignee
Harvest Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22740538&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2407346(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Harvest Technologies Corp filed Critical Harvest Technologies Corp
Publication of CA2407346A1 publication Critical patent/CA2407346A1/en
Application granted granted Critical
Publication of CA2407346C publication Critical patent/CA2407346C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • B04B7/12—Inserts, e.g. armouring plates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/02—Separation of non-miscible liquids
    • B01D17/0217—Separation of non-miscible liquids by centrifugal force
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/24—Feed or discharge mechanisms for settling tanks
    • B01D21/2433—Discharge mechanisms for floating particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
    • B01D21/262—Separation of sediment aided by centrifugal force or centripetal force by using a centrifuge
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B01L3/50215—Test tubes specially adapted for centrifugation purposes using a float to separate phases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B11/00—Feeding, charging, or discharging bowls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/02—Blood transfusion apparatus
    • A61M1/029—Separating blood components present in distinct layers in a container, not otherwise provided for
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2221/00—Applications of separation devices
    • B01D2221/10—Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Vascular Medicine (AREA)
  • Clinical Laboratory Science (AREA)
  • Veterinary Medicine (AREA)
  • Cardiology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Anesthesiology (AREA)
  • Thermal Sciences (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Ecology (AREA)
  • Centrifugal Separators (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • External Artificial Organs (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

A separator disk (4) for use in centrifugal separation of components is designed to automatically position itself during separation at the interface between the supernatant and the remaining components. Preferably the interface is between plasma and red blood cells.

Description

BLOOD COMPONENTS SEPARATOR DISK

TECHNICAL FIELD

This invention relates to methods and apparatus for use in the separation of fluids into components having different specific gravities. The invention finds particular utility in the centrifugal separation of the components of blood.

BACKGROUND
Centrifugal separation of blood into components of different specific gravities, such as red blood cells, white blood cells, platelets, and plasma is known from United States Patent 5,707,331 (Wells). The apparatus shown in that patent employs a disposable processing tube having two chambers, and blood to be separated into components is placed in one of the chambers. The processing tube is placed in a centrifuge, which subjects the blood to centrifugal forces to separate the components. The supernatant is then automatically decanted into the second of the chambers.

To retain, principally, the red blood cells during the decant of the supernatant, the apparatus disclosed in the Wells patent includes a shelf placed in the first chamber at the expected level of the interface between the red blood cells and the less-dense components, including the plasma. One problem with the arrangement shown in the `331 Wells patent, however, is that the position of the interface varies with the particular proportions of the components (e.g., the hematocrit) of the blood to be processed. Thus, if the shelf is placed at the expected position of the interface for blood of average hematocrit, and the hematocrit of the particular blood being processed is low, the shelf will be above the interface after separation. Such a position of the shelf will hinder the flow of the components near the interface during decanting, thus retaining significant amounts of these components in the first chamber and reducing the separation efficiency of the system.

SUMMARY OF THE INVENTION

In accordance with the invention, a movable separator disk, which automatically positions itself at the interface between the separated components, is placed in the first chamber. In the preferred embodiment, the disk is capable of moving vertically and is designed to position itself automatically at the interface between red blood cells and the remaining components in the centrifugal separation of blood.

Decant of the supernatant can be either by gravity drain or by centrifugal transfer, and a main function of the disk is to restrict the flow of the component below it, e.g., red blood cells, during decant. This ensures that the supernatant is not contaminated and increases the efficiency of the process.

The invention contemplates two embodiments for the disk. In one embodiment, the disk is supported on a central shaft such that an annulus is formed between the perimeter of the disk and the interior surface of the first chamber. The dimensions of the annulus are such that the flow of red blood cells through it during decant is restricted such that they do not contaminate the decanted supernatant to any significant degree.
In another embodiment, the disk is arranged on the shaft such that, when the chamber is tilted for gravity decanting, the disk rotates such that one edge of the disk engages the wall of the chamber to block flow of red blood cells.

In either of these embodiments, the specific gravity of the disk and its shape may be chosen so that a major part of the upper surface lies just below the interface, thus facilitating release of the supernatant from the disk during decanting. This upper surface is also preferably curved to match the cylindrical shape the interface assumes during centrifugation.

BRIEF DESCRIPTION OF THE DRAWINGS

Figure 1 a is a longitudinal cross-section of a portion of a processing tube chamber and a separator disk in accordance with a first embodiment of the invention.

Figure 1 b is a transverse cross section taken along line 1 b-1 b of figure 1 a.

Figure 2a is a longitudinal cross-section of the embodiment of figures 1 a and 1 b when the separator disk is tilted during decanting.

Figure 2b is a transverse cross section taken along line 2b-2b of figure 2a.

Figure 3a is a longitudinal cross-section of a second embodiment of the invention.

Figure 3b is a transverse cross section taken along line 3b-3b of figure 3a.

Figure 4 is a longitudinal cross-section of a third embodiment of the invention.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

With reference to figures 1 and 2, one chamber 2 of a processing tube, such as that shown in the `331 Wells patent has a separator disk 4 in accordance with the invention supported therein by a central shaft 6. The shaft 6 is designed to direct fluid introduced into the chamber to the bottom of the chamber. This precludes the formation of an air bubble at the bottom of the chamber, particularly when the bottom of the chamber is tapered. Thus, fluid is introduced into the chamber by inserting a cannula attached to a syringe containing blood into the shaft 6 and discharging the blood from the syringe into the chamber. A central opening 8 in the disk receives the shaft 6 in such a manner that the disk easily slides along the shaft.

The shaft 6 may not be necessary in all instances, for example, when the bottom of the processing tube is flat. In that instance the disk does not have a central hole.

The disk is preferably made of material having a specific gravity that allows the disk to float at the interface with red blood cells. In the preferred embodiment that specific gravity is about 1.04 (e.g., polystyrene), which is just less than the specific gravity of red blood cells at 70% hematocrit. Thus, when the blood is centrifuged, the disk moves to the interface between the red blood cells and the other components.

The interface will naturally assume a cylindrical shape with a cylindrical radius equal to the distance to the center of rotation of the centrifuge. The disk may be cylindrical, to match the shape of the interface.

In the embodiment shown in figures 1 a, 1 b, 2a and 2b, the diameters of the hole 8 and the shaft 6 are such that an annular gap 10 is formed between the outer surface of the shaft and the interior surface of the hole 8.
Similarly, an annular gap 12 is provided between the perimeter of the disk and the interior surface of the tube 2.

Figures 1 a and 1 b illustrate the position of the disk during centrifugation, and it will be appreciated that the gaps 10 and 12 are large enough to allow passage of the descending heavier components, e.g., red blood cells and the ascending lighter components, e.g., plasma. According to this embodiment, however, the diameter of the central opening 8 is large enough whereby during decanting the disk 4 rotates as shown in the figures.
Thus, when the processing tube is rotated to the decant position, the more dense red blood cells, illustrated at 14, that have accumulated below the disk exert a force against the bottom of the disk as they try to flow through the gap 12. This causes the disk 4 to rotate, as shown in figures 2a and 2b, until a portion of the lower outer edge 16 of the disk and also the upper outer edge 18 engage the inner surface of the chamber 2. This engagement between the edge 16 of the disk and the interior of the chamber effectively forms a valve that prevents flow of the red blood cells, allowing decant of the plasma supernatant without contamination by red blood cells. It will be appreciated that this embodiment requires the transverse dimension of the disk between edges 16 and 18 to be greater than the internal diameter of the tube so that the edges engage the interior of the tube when tilted.

A second embodiment is shown in figures 3a and 3b. According to this embodiment, the gap 10 is made to be small whereby the disk does not rotate appreciably during decant, in contrast to the embodiment of figures 1 and 2.
It will be appreciated that an annular channel is formed by the gap 12, this channel having a width equal to the radial dimension of the gap and a length equal to the thickness of the disk at the edge. The rate of flow of a fluid through this channel is a function of the dimensions of the channel, and the dimensions of the disk of this embodiment are such that the red blood cells will not flow appreciably through the channel at 1 G. In the preferred embodiment, the width of the gap is about 0.005 inch to about 0.020 inch, and the length is about 0.1 inch to about 0.3 inch.

Thus, the components of the blood flow through the channel during centrifugation (i.e., at 1000G), but do not flow appreciably through the channel during decanting at 1 G. This allows the supernatant to be decanted without significant contamination by the red blood cells.

Figure 4 illustrates a preferred shape of the disk 4. In this embodiment, the top surface 20 of the disk is concave, preferably cylindrical, and the disk is provided with an elongated central portion 22. The specific gravity of the disk material is selected so that the concave surface 20 is located just below the interface. That is, the thickness of the outer edge, the length of the portion 22, and the specific gravity of the material are chosen so that the center of buoyancy of the disk is just above the concave surface, and that surface will be just below the interface 26 with red blood cells. This arrangement allows a small layer 24 of the red blood cells to form on the upper surface.

The layer of red blood cells 24 reduces the surface tension between the platelets at the interface 26 and the surface 20 of the disk and facilitates release of the platelets from the disk. This is important to ensure that all of the platelets are decanted, and the small amount of red blood cells that may be decanted along with the supernatant does not generally represent a significant contamination of the supernatant.

Modifications within the scope of the appended claims will be apparent to those of skill in the art.

Claims (12)

CLAIMS:
1. A combination comprising a tube comprising an inner wall forming a cavity adapted to contain a physiological fluid to be subjected to centrifugation and a separator disk in said tube adapted to float freely in said physiological fluid and separate components of said physiological fluid having different specific gravities, said disk being made of a material having a specific gravity near the specific gravity of a first component of said physiological fluid and comprising a peripheral portion adjacent said inner wall of said tube and a central portion extending across at least a portion of said cavity, said central portion having an upper surface forming a closed recess configured such that after centrifugation of said physiological fluid said closed recess lies just below an interface formed by said first component that has been separated from other of said components and accumulates a small layer of said first component.
2. A combination according to claim 1 wherein said disk fits into said tube such that a gap is formed between the perimeter of said disk and the interior of said tube, said gap being of such a dimension that the component of said fluid below said disk after separation will not flow through said gap at about 1G.
3. A combination according to claim 1 further comprising a shaft extend-ing along said tube and engaging said disk such that said disk slides along said shaft.
4. A combination according to claim 1 wherein said disk is arranged to rotate about an axis transverse to the longitudinal axis of said tube to form a valve with the side of said tube during decanting.
5. A combination according to claim 4 further comprising a shaft extend-ing along said tube and engaging said disk such that said disk slides along said shaft.
6. A combination according to claim 4 wherein said disk includes an upper outer edge and a lower outer edge and the transverse dimension between said upper outer edge and lower outer edge is greater than the internal diameter of said tube.
7. A combination according to claim 1 wherein said upper surface is curved.
8. A combination according to claim 7 wherein said upper surface is cylindrical.
9. A combination according to claim 1 wherein said disk is shaped such that its center of buoyancy is located above said upper surface of the disk.
10. A combination according to claim 1 wherein said physiological fluid is blood and the specific gravity of said disk is such that said upper surface lies just below an interface between plasma and red blood cells.
11. A method for separating a supernatant from a first component of a physiological fluid comprising the steps of:
providing a tube comprising an inner wall forming a cavity adapted to contain a physiological fluid to be subjected to centrifugation and a separator disk in said tube adapted to float freely in said physiological fluid and separate components of said physiological fluid having different specific gravities, said disk being made of a material having a specific gravity near the specific gravity of a first component of said physiological fluid and comprising a peripheral portion adjacent said inner wall of said tube and a central portion extending across at least a portion of said cavity, said central portion having an upper surface forming a closed recess configured such that after centrifugation of said physiological fluid said closed recess lies just below an interface formed by said first component that has been separated from other of said components and accumulates a small layer of said first component, providing said physiological fluid to the container, and subjecting said container and said physiological fluid to centrifugation.
12. A method according to claim 11 wherein said physiological fluid is blood and said first component is red blood cells.
CA002407346A 2000-04-28 2001-04-27 Blood components separator disk Expired - Fee Related CA2407346C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20015000P 2000-04-28 2000-04-28
US60/200,150 2000-04-28
PCT/US2001/011732 WO2001083068A1 (en) 2000-04-28 2001-04-27 Blood components separator disk

Publications (2)

Publication Number Publication Date
CA2407346A1 CA2407346A1 (en) 2001-11-08
CA2407346C true CA2407346C (en) 2009-09-01

Family

ID=22740538

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002407346A Expired - Fee Related CA2407346C (en) 2000-04-28 2001-04-27 Blood components separator disk

Country Status (14)

Country Link
US (8) US7077273B2 (en)
EP (1) EP1289618B1 (en)
JP (1) JP4128007B2 (en)
CN (1) CN1309442C (en)
AT (1) ATE382408T1 (en)
AU (2) AU2001272085B2 (en)
CA (1) CA2407346C (en)
CY (1) CY1107189T1 (en)
DE (1) DE60132198T2 (en)
DK (1) DK1289618T3 (en)
ES (1) ES2298234T3 (en)
HK (1) HK1059059A1 (en)
PT (1) PT1289618E (en)
WO (1) WO2001083068A1 (en)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7947236B2 (en) 1999-12-03 2011-05-24 Becton, Dickinson And Company Device for separating components of a fluid sample
CN1309442C (en) 2000-04-28 2007-04-11 丰收技术股份有限公司 Blood components separator disc
US7832566B2 (en) 2002-05-24 2010-11-16 Biomet Biologics, Llc Method and apparatus for separating and concentrating a component from a multi-component material including macroparticles
US7992725B2 (en) 2002-05-03 2011-08-09 Biomet Biologics, Llc Buoy suspension fractionation system
US20030205538A1 (en) 2002-05-03 2003-11-06 Randel Dorian Methods and apparatus for isolating platelets from blood
US7845499B2 (en) 2002-05-24 2010-12-07 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
DE10392686T5 (en) 2002-05-24 2005-07-07 Biomet Mfg. Corp., Warsaw Apparatus and method for separating and concentrating liquids containing multiple components
US20060278588A1 (en) 2002-05-24 2006-12-14 Woodell-May Jennifer E Apparatus and method for separating and concentrating fluids containing multiple components
WO2004026709A1 (en) * 2002-09-19 2004-04-01 Harvest Technologies Corporation Sterile disposable unit
US7354515B2 (en) 2004-02-23 2008-04-08 Millennium Medical Technologies, Inc. Fluid concentrator
WO2006086201A2 (en) 2005-02-07 2006-08-17 Hanuman Llc Platelet rich plasma concentrate apparatus and method
US7866485B2 (en) 2005-02-07 2011-01-11 Hanuman, Llc Apparatus and method for preparing platelet rich plasma and concentrates thereof
JP4961354B2 (en) 2005-02-07 2012-06-27 ハヌマン リミテッド ライアビリティ カンパニー Platelet rich plasma concentration apparatus and method
US8048297B2 (en) 2005-08-23 2011-11-01 Biomet Biologics, Llc Method and apparatus for collecting biological materials
US7771590B2 (en) 2005-08-23 2010-08-10 Biomet Manufacturing Corp. Method and apparatus for collecting biological materials
US8567609B2 (en) 2006-05-25 2013-10-29 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US7767087B2 (en) 2007-01-05 2010-08-03 Wilson Kelce S Floating filter holder
US8328024B2 (en) 2007-04-12 2012-12-11 Hanuman, Llc Buoy suspension fractionation system
US7806276B2 (en) 2007-04-12 2010-10-05 Hanuman, Llc Buoy suspension fractionation system
JP2010531142A (en) * 2007-06-22 2010-09-24 サークル バイオロジクス、 エルエルシー． Liquid concentrator, autologous concentrated body fluid, and methods of use thereof
CN101952006A (en) * 2007-12-07 2011-01-19 丰收技术股份有限公司 Floating disk for separating blood components
EP2259774B1 (en) 2008-02-27 2012-12-12 Biomet Biologics, LLC Methods and compositions for delivering interleukin-1 receptor antagonist
US8337711B2 (en) 2008-02-29 2012-12-25 Biomet Biologics, Llc System and process for separating a material
US8012077B2 (en) 2008-05-23 2011-09-06 Biomet Biologics, Llc Blood separating device
CA2731156C (en) 2008-07-21 2013-09-24 Becton, Dickinson And Company Density phase separation device
ES2452534T3 (en) 2008-07-21 2014-04-01 Becton, Dickinson And Company Density phase separation device
CN102149473B (en) 2008-07-21 2014-12-31 贝克顿·迪金森公司 Density phase separation device
US8177072B2 (en) * 2008-12-04 2012-05-15 Thermogenesis Corp. Apparatus and method for separating and isolating components of a biological fluid
US8187475B2 (en) 2009-03-06 2012-05-29 Biomet Biologics, Llc Method and apparatus for producing autologous thrombin
US8313954B2 (en) 2009-04-03 2012-11-20 Biomet Biologics, Llc All-in-one means of separating blood components
PL3821980T3 (en) * 2009-05-15 2023-02-20 Becton, Dickinson And Company Density phase separation device
AU2015204357B2 (en) * 2009-05-15 2017-05-04 Becton, Dickinson And Company Density phase separation device
EP2435108B1 (en) 2009-05-29 2016-12-07 EndoCellutions, Inc. Apparatus and methods for aspirating and separating components of different densities from a physiological fluid containing cells
US9011800B2 (en) 2009-07-16 2015-04-21 Biomet Biologics, Llc Method and apparatus for separating biological materials
US8313644B2 (en) * 2010-01-13 2012-11-20 OZOlab Bottle with an integrated filtration assembly that is manually operated using a plunger
DE102010003224B4 (en) * 2010-03-24 2022-11-03 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Mixer for insertion in a rotor of a centrifuge
US8591391B2 (en) 2010-04-12 2013-11-26 Biomet Biologics, Llc Method and apparatus for separating a material
US9555171B2 (en) 2010-09-30 2017-01-31 Depuy Mitek, Llc Methods and devices for collecting separate components of whole blood
US20120142514A1 (en) * 2010-12-02 2012-06-07 Medikan Co., Ltd. Syringe for specific gravity distinction and fat tissue components separating method therewith
US9011684B2 (en) 2011-03-07 2015-04-21 Spinesmith Holdings, Llc Fluid concentrator with removable cartridge
KR101170028B1 (en) * 2011-12-20 2012-08-01 (주) 레보메드 Blood separating apparatus
US9642956B2 (en) 2012-08-27 2017-05-09 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US10603665B2 (en) 2013-01-29 2020-03-31 Endocellutions, Inc. Cell concentration devices and methods that include an insert defining a lumen and a cannula assembly
US20140271589A1 (en) 2013-03-15 2014-09-18 Biomet Biologics, Llc Treatment of collagen defects using protein solutions
US9895418B2 (en) 2013-03-15 2018-02-20 Biomet Biologics, Llc Treatment of peripheral vascular disease using protein solutions
US10143725B2 (en) 2013-03-15 2018-12-04 Biomet Biologics, Llc Treatment of pain using protein solutions
US9950035B2 (en) 2013-03-15 2018-04-24 Biomet Biologics, Llc Methods and non-immunogenic compositions for treating inflammatory disorders
US10208095B2 (en) 2013-03-15 2019-02-19 Biomet Manufacturing, Llc Methods for making cytokine compositions from tissues using non-centrifugal methods
US9382106B2 (en) * 2013-07-19 2016-07-05 Norman Werbner Information Services, Inc. Liquid handling system with reduced exposure to air
PL3212332T3 (en) 2014-10-28 2021-08-09 Arteriocyte Medical Systems, Inc. Centrifuge tube comprising a floating buoy, and methods for using the same
US9694359B2 (en) 2014-11-13 2017-07-04 Becton, Dickinson And Company Mechanical separator for a biological fluid
US9713810B2 (en) 2015-03-30 2017-07-25 Biomet Biologics, Llc Cell washing plunger using centrifugal force
US9757721B2 (en) 2015-05-11 2017-09-12 Biomet Biologics, Llc Cell washing plunger using centrifugal force
US10154745B2 (en) * 2016-01-27 2018-12-18 Daniel J. Noblitt Methods and apparatus for liquid preservation
US10384841B2 (en) 2017-06-29 2019-08-20 Norman Werbner Information Services, Inc. Liquid extraction, storage, and dispensing system and method of use
EP3784363A4 (en) 2018-07-09 2022-02-16 Hanuman Pelican, Inc. Apparatus and methods for separating blood components
JP2021531059A (en) 2018-07-09 2021-11-18 ハヌマン ペリカン，インコーポレイテッド Equipment and methods for processing blood
CA3127191A1 (en) 2019-01-21 2020-07-30 Eclipse Medcorp, Llc Methods, systems and apparatus for separating components of a biological sample
WO2020163105A1 (en) 2019-02-06 2020-08-13 Hanuman Pelican, Inc. Apparatus and methods for concentrating platelet-rich plasma
CA3159372A1 (en) 2019-10-31 2021-05-06 Eclipse Medcorp, Llc Systems, methods and apparatus for separating components of a sample

Family Cites Families (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US593333A (en) * 1897-11-09 Device for separating liquids of different
US10140A (en) * 1853-10-18 Smproved life-preserving bucket
US280820A (en) * 1883-07-10 Milk-can
US1818924A (en) * 1928-09-04 1931-08-11 Basmadjian Aronseag Cover for pickles, etc.
US3256977A (en) * 1965-04-09 1966-06-21 Pettersen Gunnar Nimrod Filled packaging and dispensing container
US3409165A (en) * 1967-04-03 1968-11-05 Olin Mathieson Floating deck
US3508653A (en) * 1967-11-17 1970-04-28 Charles M Coleman Method and apparatus for fluid handling and separation
US3618810A (en) * 1969-10-21 1971-11-09 Wilson Henry A Shaving lather moistening and heating device
US3647070A (en) 1970-06-11 1972-03-07 Technicon Corp Method and apparatus for the provision of fluid interface barriers
US3661265A (en) 1970-07-27 1972-05-09 Contemporary Research And Dev Serum separator type container
US3814248A (en) 1971-09-07 1974-06-04 Corning Glass Works Method and apparatus for fluid collection and/or partitioning
US3852194A (en) 1972-12-11 1974-12-03 Corning Glass Works Apparatus and method for fluid collection and partitioning
JPS517859B2 (en) 1973-01-20 1976-03-11
US3814258A (en) 1973-03-15 1974-06-04 Dickinson And Co Blood plasma separator with filter
US4001122A (en) 1973-08-22 1977-01-04 Telan Corporation Method and device for separating blood components
FR2249265B1 (en) 1973-10-25 1980-06-27 Gele Pierre
US3894951A (en) 1974-02-27 1975-07-15 Becton Dickinson Co Serum/plasma separator; interface seeking piston; resilient apertures in lower diaphragm type
US3887466A (en) 1974-02-27 1975-06-03 Becton Dickinson Co Serum/plasma separator cannula fluid by-pass type centrifugal valve cannula seal
US3897337A (en) 1974-02-27 1975-07-29 Becton Dickinson Co Plasma separator assembly having interface-seeking piston with centrifugal valve
US3941699A (en) 1974-02-27 1976-03-02 Becton, Dickinson And Company Plasma separator with centrifugal valve
US3945928A (en) 1974-02-27 1976-03-23 Becton, Dickinson And Company Serum/plasma separators with centrifugal valves
US3909419A (en) * 1974-02-27 1975-09-30 Becton Dickinson Co Plasma separator with squeezed sealant
US3951801A (en) 1974-02-27 1976-04-20 Becton, Dickinson And Company Serum/plasma separator-strut stop type
US3957654A (en) 1974-02-27 1976-05-18 Becton, Dickinson And Company Plasma separator with barrier to eject sealant
US3919085A (en) 1974-02-27 1975-11-11 Becton Dickinson Co Plasma separator assembly
US3894950A (en) 1974-02-27 1975-07-15 Becton Dickinson Co Serum separator improvement with stretchable filter diaphragm
US3894952A (en) 1974-02-27 1975-07-15 Becton Dickinson Co Serum/plasma separator assembly having interface-seeking piston
US3935113A (en) 1974-02-27 1976-01-27 Becton, Dickinson And Company Serum/plasma separator with centrifugal valve
US3897343A (en) 1974-02-27 1975-07-29 Becton Dickinson Co Plasma separator-hydrostatic pressure type
US3931010A (en) 1974-02-27 1976-01-06 Becton, Dickinson And Company Serum/plasma separators with centrifugal valves
US3920557A (en) 1974-02-27 1975-11-18 Becton Dickinson Co Serum/plasma separator--beads-plus-adhesive type
US3929646A (en) 1974-07-22 1975-12-30 Technicon Instr Serum separator and fibrin filter
US3931018A (en) 1974-08-09 1976-01-06 Becton, Dickinson And Company Assembly for collection, separation and filtration of blood
US3972812A (en) * 1975-05-08 1976-08-03 Becton, Dickinson And Company Blood serum separation filter disc
US4083788A (en) * 1975-11-19 1978-04-11 Ferrara Louis T Blood serum-isolation device
US4180465A (en) 1975-12-19 1979-12-25 Sherwood Medical Industries Inc. Fluid collection device with phase separation means
US4055501A (en) 1976-01-16 1977-10-25 Sherwood Medical Industries Inc. Fluid collection device with phase partitioning means
US4088582A (en) 1976-01-16 1978-05-09 Sherwood Medical Industries Inc. Blood phase separation means
JPS52126613A (en) 1976-04-16 1977-10-24 Kubota Ltd Heat-resisting cast alloy
CA1074273A (en) 1976-05-06 1980-03-25 Sherwood Medical Industries Inc. Phase separation device
AT381466B (en) 1977-03-16 1986-10-27 Ballies Uwe SEPARATING TUBES FOR CENTRIFUGAL SEPARATION
SE7710076L (en) 1977-09-08 1979-03-09 Ericson Curt BLOOD SAMPLING CONTAINER
US4169060A (en) 1977-10-25 1979-09-25 Eastman Kodak Company Blood-collecting and serum-dispensing device
JPS5917386B2 (en) 1979-03-23 1984-04-20 テルモ株式会社 Blood separation method and device
SE416378B (en) 1979-03-28 1980-12-22 Johansson A S SET ON SEPARATION OF BLOOD COMPONENTS FROM WHOLE BLOOD APPLICABLE BLOOD PASS SYSTEM FOR EXECUTIVE DEVICE SET
US4310430A (en) 1979-09-11 1982-01-12 Terumo Corporation α-Olefin-dialkylmaleate-based liquid separating agent
US4279863A (en) * 1979-09-12 1981-07-21 Sherwood Medical Industries, Inc. Reagent separator for a blood collection tube
US4369117A (en) 1980-05-12 1983-01-18 American Hospital Supply Corporation Serum separating method and apparatus
JPS56168814A (en) 1980-06-02 1981-12-25 Terumo Corp Barrier for separation of blood
DE3101733C2 (en) * 1981-01-21 1982-10-14 Uwe Dr.Med. 2300 Kiel Ballies Separating element in a separating tube for centrifugal separation
US4707276A (en) 1981-04-15 1987-11-17 Sherwood Medical Company Fluid collection device with phase partitioning means
US4417981A (en) * 1981-05-04 1983-11-29 Becton, Dickinson And Company Blood phase separator device
US4443345A (en) 1982-06-28 1984-04-17 Wells John R Serum preparator
US4492634A (en) 1982-09-28 1985-01-08 Emde Medical Research Pre-evacuated blood collection tube with anti-hemolysis baffle system and centrifugation propelled filtration disc and efficient serum-from cells separator
US4487700A (en) 1983-02-18 1984-12-11 Technicon Instruments Corporation Method and apparatus for separating lymphocytes from anticoagulated blood
US4563332A (en) * 1983-04-27 1986-01-07 Icl Scientific, Inc. Liquid sampling apparatus with retention means
DE3343887A1 (en) 1983-12-05 1985-06-13 Walter Sarstedt Kunststoff-Spritzgußwerk, 5223 Nümbrecht ARRANGEMENT FOR PLACING A SEPARATOR BETWEEN TWO PHASES IN A SAMPLE TUBE
US4751001A (en) 1984-09-24 1988-06-14 Becton Dickinson And Company Blood partitioning apparatus
US5053134A (en) * 1984-12-04 1991-10-01 Becton Dickinson And Company Lymphocyte collection tube
IL74967A (en) 1985-04-18 1988-10-31 Assaf Pharmaceutical Ind Separation of materials from a liquid dispersion by sedimentation
SE448323B (en) 1985-08-27 1987-02-09 Ersson Nils Olof PROCEDURE AND PROCEDURE TO SEPARATE SERUM OR PLASMA FROM BLOOD
JPH0677014B2 (en) 1987-08-14 1994-09-28 テルモ株式会社 Blood separation tube
US4818386A (en) 1987-10-08 1989-04-04 Becton, Dickinson And Company Device for separating the components of a liquid sample having higher and lower specific gravities
US4877520A (en) 1987-10-08 1989-10-31 Becton, Dickinson And Company Device for separating the components of a liquid sample having higher and lower specific gravities
US4844818A (en) * 1987-10-23 1989-07-04 Becton Dickinson & Company Method for separating the cellular components of blood samples
US4989403A (en) 1988-05-23 1991-02-05 Sundstrand Corporation Surge protected gas turbine engine for providing variable bleed air flow
US4946601A (en) 1988-08-22 1990-08-07 Sherwood Medical Company Blood serum separator tube
US4946604A (en) * 1988-11-23 1990-08-07 Halliburton Company Method for treating a well bore
US4954264A (en) 1989-02-02 1990-09-04 Becton-Dickinson And Company Apparatus for separating mononuclear cells from blood and method of manufacturing and using the same
DE8910591U1 (en) 1989-09-05 1989-12-21 Walter Sarstedt Geräte und Verbrauchsmaterial für Medizin und Wissenschaft, 5223 Nümbrecht Blood serum collection device
AU6870091A (en) 1989-11-08 1991-06-13 Fmc Corporation Combined centrifuge tube and porous selection means for separation and recovery of biological materials
JPH0774772B2 (en) 1990-12-31 1995-08-09 エイ． レビン ロバート Blood sampling assembly, target cell collection method and target component collection method
US5269927A (en) 1991-05-29 1993-12-14 Sherwood Medical Company Separation device for use in blood collection tubes
US5316779A (en) * 1991-09-16 1994-05-31 Morey Booker W Foam-limiting drinking cup and method
IL100828A (en) 1992-01-31 2002-05-23 Novamed Ltd Method and means for density gradient centrifugation
US5282981A (en) 1992-05-01 1994-02-01 E. I. Du Pont De Nemours And Company Flow restrictor-separation device
US5389265A (en) 1993-06-02 1995-02-14 E. I. Du Pont De Nemours And Company Phase-separation tube
US5456885A (en) 1993-07-12 1995-10-10 Coleman; Charles M. Fluid collection, separation and dispensing tube
JPH07103969A (en) 1993-08-13 1995-04-21 Niigata Kako Kk Blood separation member and blood collecting tube for blood separation
US5533518A (en) 1994-04-22 1996-07-09 Becton, Dickinson And Company Blood collection assembly including mechanical phase separating insert
US5577513A (en) 1994-08-31 1996-11-26 Activated Cell Therapy, Inc. Centrifugation syringe, system and method
US5840502A (en) 1994-08-31 1998-11-24 Activated Cell Therapy, Inc. Methods for enriching specific cell-types by density gradient centrifugation
PL320512A1 (en) * 1994-12-02 1997-10-13 Bristol Myers Squibb Co System for feeding a reagent into a centrifuge
US5560830A (en) 1994-12-13 1996-10-01 Coleman; Charles M. Separator float and tubular body for blood collection and separation and method of use thereof
US5707331A (en) * 1995-05-05 1998-01-13 John R. Wells Automatic multiple-decanting centrifuge
US5632905A (en) * 1995-08-07 1997-05-27 Haynes; John L. Method and apparatus for separating formed and unformed components
US5736033A (en) * 1995-12-13 1998-04-07 Coleman; Charles M. Separator float for blood collection tubes with water swellable material
US5707876A (en) * 1996-03-25 1998-01-13 Stephen C. Wardlaw Method and apparatus for harvesting constituent layers from a centrifuged material mixture
US5785925A (en) 1996-08-29 1998-07-28 Saigene Corporation Centrifuge tube phase separation plug
US5889584A (en) 1997-03-10 1999-03-30 Robert A. Levine Assembly for rapid measurement of cell layers
US5860937A (en) 1997-04-30 1999-01-19 Becton, Dickinson & Company Evacuated sample collection tube with aqueous additive
US5918622A (en) 1997-07-01 1999-07-06 Bermad Separation valve
US6406671B1 (en) * 1998-12-05 2002-06-18 Becton, Dickinson And Company Device and method for separating components of a fluid sample
US6280400B1 (en) 1998-12-05 2001-08-28 Becton Dickinson And Company Device and method for separating component of a liquid sample
US6409528B1 (en) 1999-12-06 2002-06-25 Becton, Dickinson And Company Device and method for collecting, preparation and stabilizing a sample
JP3619933B2 (en) 2000-03-02 2005-02-16 アークレイ株式会社 Centrifuge container
US20010031688A1 (en) * 2000-04-18 2001-10-18 Anderson Norman G. Method and apparatus for making density gradients
CN1309442C (en) * 2000-04-28 2007-04-11 丰收技术股份有限公司 Blood components separator disc

Also Published As

Publication number Publication date
CN1441690A (en) 2003-09-10
US7077273B2 (en) 2006-07-18
DK1289618T3 (en) 2008-04-28
US20170008012A1 (en) 2017-01-12
US20130079212A1 (en) 2013-03-28
EP1289618B1 (en) 2008-01-02
EP1289618A1 (en) 2003-03-12
US9393576B2 (en) 2016-07-19
US9393575B2 (en) 2016-07-19
DE60132198D1 (en) 2008-02-14
USRE43547E1 (en) 2012-07-24
DE60132198T2 (en) 2008-12-18
AU7208501A (en) 2001-11-12
CA2407346A1 (en) 2001-11-08
US20030010711A1 (en) 2003-01-16
ATE382408T1 (en) 2008-01-15
JP2003531704A (en) 2003-10-28
US20060032825A1 (en) 2006-02-16
US20150290661A1 (en) 2015-10-15
WO2001083068A1 (en) 2001-11-08
JP4128007B2 (en) 2008-07-30
HK1059059A1 (en) 2004-06-18
EP1289618A4 (en) 2003-05-14
US7547272B2 (en) 2009-06-16
AU2001272085B2 (en) 2006-03-02
US20090283524A1 (en) 2009-11-19
US9656274B2 (en) 2017-05-23
ES2298234T3 (en) 2008-05-16
PT1289618E (en) 2008-04-11
CN1309442C (en) 2007-04-11
CY1107189T1 (en) 2012-10-24
US20140131292A1 (en) 2014-05-15

Similar Documents

Publication Publication Date Title
CA2407346C (en) Blood components separator disk
AU2001272085A1 (en) Blood components separator disk
US8348066B2 (en) Floating disk for separating blood components
US20070262034A1 (en) Particle sedimentation apparatus and method for performing particle sedimentation
JPS63500679A (en) Method and apparatus for separating serum/plasma from blood
US3882021A (en) Sealed assembly for separation of blood with anti-red cell barrier
CA2024892C (en) Blood collection method
CN108367302B (en) Centrifugal separator and centrifugal separation method
PL125115B1 (en) Centrifugal separator
KR102508907B1 (en) Chamber for centrifuge device and centrifuge device comprising the same
US20240197976A1 (en) Blood plasma separation device
EP4048444B1 (en) Device for controlled layering of liquids
CA1327557C (en) Plasma separator
WO2024039424A1 (en) Float securement apparatus and methods

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20150427