AU2001272085A1 - Blood components separator disk - Google Patents
Blood components separator diskInfo
- Publication number
- AU2001272085A1 AU2001272085A1 AU2001272085A AU2001272085A AU2001272085A1 AU 2001272085 A1 AU2001272085 A1 AU 2001272085A1 AU 2001272085 A AU2001272085 A AU 2001272085A AU 2001272085 A AU2001272085 A AU 2001272085A AU 2001272085 A1 AU2001272085 A1 AU 2001272085A1
- Authority
- AU
- Australia
- Prior art keywords
- disk
- tube
- fluid
- supernatant
- combination according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Description
BLOOD COMPONENTS SEPARATOR DISK
TECHNICAL FIELD
This invention relates to methods and apparatus for use in the separation of fluids into components having different specific gravities. The invention finds particular utility in the centrifugal separation of the components of blood.
BACKGROUND
Centrifugal separation of blood into components of different specific gravities, such as red blood cells, white blood cells, platelets, and plasma is known from United States Patent 5,707,331 (Wells). The apparatus shown in that patent employs a disposable processing tube having two chambers, and blood to be separated into components is placed in one of the chambers. The processing tube is placed in a centrifuge, which subjects the blood to centrifugal forces to separate the components. The supernatant is then automatically decanted into the second of the chambers.
To retain, principally, the red blood cells during the decant of the supernatant, the apparatus disclosed in the Wells patent includes a shelf placed in the first chamber at the expected level of the interface between the red blood cells and the less-dense components, including the plasma. One problem with the arrangement shown in the '331 Wells patent, however, is that the position of the interface varies with the particular proportions of the components (e.g., the hematocrit) of the blood to be processed. Thus, if the
shelf is placed at the expected position of the interface for blood of average hematocrit, and the hematocrit of the particular blood being processed is low, the shelf will be above the interface after separation. Such a position of the shelf will hinder the flow of the components near the interface during decanting, thus retaining significant amounts of these components in the first chamber and reducing the separation efficiency of the system.
SUMMARY OF THE INVENTION
In accordance with the invention, a movable separator disk, which automatically positions itself at the interface between the separated components, is placed in the first chamber. In the preferred embodiment, the disk is capable of moving vertically and is designed to position itself automatically at the interface between red blood cells and the remaining components in the centrifugal separation of blood.
Decant of the supernatant can be either by gravity drain or by centrifugal transfer, and a main function of the disk is to restrict the flow of the component below it, e.g., red blood cells, during decant. This ensures that the supernatant is not contaminated and increases the efficiency of the process.
The invention contemplates two embodiments for the disk. In one embodiment, the disk is supported on a central shaft such that an annulus is formed between the perimeter of the disk and the interior surface of the first chamber. The dimensions of the annulus are such that the flow of red blood cells through it during decant is restricted such that they do not contaminate the decanted supernatant to any significant degree.
In another embodiment, the disk is arranged on the shaft such that, when the chamber is tilted for gravity decanting, the disk rotates such that one edge of the disk engages the wall of the chamber to block flow of red blood cells.
In either of these embodiments, the specific gravity of the disk and its shape may be chosen so that a major part of the upper surface lies just below the interface, thus facilitating release of the supernatant from the disk during decanting. This upper surface is also preferably curved to match the cylindrical shape the interface assumes during centrifugation.
BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1 a is a longitudinal cross-section of a portion of a processing tube chamber and a separator disk in accordance with a first embodiment of the invention.
Figure 1 b is a transverse cross section taken along line 1 b-1 b of figure 1 a.
Figure 2a is a longitudinal cross-section of the embodiment of figures 1 a and 1 b when the separator disk is tilted during decanting.
Figure 2b is a transverse cross section taken along line 2b-2b of figure 2a.
Figure 3a is a longitudinal cross-section of a second embodiment of the invention.
Figure 3b is a transverse cross section taken along line 3b-3b of figure 3a.
Figure 4 is a longitudinal cross-section of a third embodiment of the invention.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
With reference to figures 1 and 2, one chamber 2 of a processing tube, such as that shown in the '331 Wells patent has a separator disk 4 in accordance with the invention supported therein by a central shaft 6. The shaft 6 is designed to direct fluid introduced into the chamber to the bottom of the chamber. This precludes the formation of an air bubble at the bottom of the chamber, particularly when the bottom of the chamber is tapered. Thus, fluid is introduced into the chamber by inserting a cannula attached to a syringe containing blood into the shaft 6 and discharging the blood from the syringe into the chamber. A central opening 8 in the disk receives the shaft 6 in such a manner that the disk easily slides along the shaft.
The shaft 6 may not be necessary in all instances, for example, when the bottom of the processing tube is flat. In that instance the disk does not have a central hole.
The disk is preferably made of material having a specific gravity that allows the disk to float at the interface with red blood cells. In the preferred embodiment that specific gravity is about 1.04 (e.g., polystyrene), which is just less than the specific gravity of red blood cells at 70% hematocrit. Thus, when the blood is centrifuged, the disk moves to the interface between the red blood cells and the other components.
The interface will naturally assume a cylindrical shape with a cylindrical radius equal to the distance to the center of rotation of the centrifuge. The disk may be cylindrical, to match the shape of the interface.
In the embodiment shown in figures 1 a, 1 b, 2a and 2b, the diameters of the hole 8 and the shaft 6 are such that an annular gap 10 is formed between
the outer surface of the shaft and the interior surface of the hole 8. Similarly, an annular gap 12 is provided between the perimeter of the disk and the interior surface of the tube 2.
Figures 1 a and 1 b illustrate the position of the disk during centrifugation, and it will be appreciated that the gaps 10 and 12 are large enough to allow passage of the descending heavier components, e.g., red blood cells and the ascending lighter components, e.g., plasma. According to this embodiment, however, the diameter of the central opening 8 is large enough whereby during decanting the disk 4 rotates as shown in the figures. Thus, when the processing tube is rotated to the decant position, the more dense red blood cells, illustrated at 14, that have accumulated below the disk exert a force against the bottom of the disk as they try to flow through the gap 12. This causes the disk 4 to rotate, as shown in figures 2a and 2b, until a portion of the lower outer edge 16 of the disk and also the upper outer edge 18 engage the inner surface of the chamber 2. This engagement between the edge 16 of the disk and the interior of the chamber effectively forms a valve that prevents flow of the red blood cells, allowing decant of the plasma supernatant without contamination by red blood cells. It will be appreciated that this embodiment requires the transverse dimension of the disk between edges 16 and 18 to be greater than the internal diameter of the tube so that the edges engage the interior of the tube when tilted.
A second embodiment is shown in figures 3a and 3b. According to this embodiment, the gap 10 is made to be small whereby the disk does not rotate appreciably during decant, in contrast to the embodiment of figures 1 and 2. It will be appreciated that an annular channel is formed by the gap 12, this
channel having a width equal to the radial dimension of the gap and a length equal to the thickness of the disk at the edge. The rate of flow of a fluid through this channel is a function of the dimensions of the channel, and the dimensions of the disk of this embodiment are such that the red blood cells will not flow appreciably through the channel at 1 G. In the preferred embodiment, the width of the gap is about 0.005 inch to about 0.020 inch, and the length is about 0.1 inch to about 0.3 inch.
Thus, the components of the blood flow through the channel during centrifugation (i.e., at 1000G), but do not flow appreciably through the channel during decanting at 1 G. This allows the supernatant to be decanted without significant contamination by the red blood cells.
Figure 4 illustrates a preferred shape of the disk 4. In this embodiment, the top surface 20 of the disk is concave, preferably cylindrical, and the disk is provided with an elongated central portion 22. The specific gravity of the disk material is selected so that the concave surface 20 is located just below the interface. That is, the thickness of the outer edge, the length of the portion 22, and the specific gravity of the material are chosen so that the center of buoyancy of the disk is just above the concave surface, and that surface will be just below the interface 26 with red blood cells. This arrangement allows a small layer 24 of the red blood cells to form on the upper surface.
The layer of red blood cells 24 reduces the surface tension between the platelets at the interface 26 and the surface 20 of the disk and facilitates release of the platelets from the disk. This is important to ensure that all of the platelets are decanted, and the small amount of red blood cells that may
be decanted along with the supernatant does not generally represent a significant contamination of the supernatant.
Modifications within the scope of the appended claims will be apparent to those of skill in the art.
Claims (16)
1. In combination, a tube adapted to contain a fluid to be subjected to centrifugation and a separator disk in said tube adapted to separate components of said fluid having different specific gravities, said disk being made of a material having a specific gravity near the specific gravity of the components at an interface.
2. A combination according to claim 1 wherein said disk fits into said tube such that a gap is formed between the perimeter of said disk and the interior of said tube, said gap being of such a dimension that the component of said below said disk after separation will not flow through said gap at about
1 G.
3. A combination according to claim 1 further comprising a shaft extending along said tube and engaging said disk such that said disk slides along said shaft.
4. A combination according to claim 1 wherein said disk is arranged to rotate about an axis transverse to the longitudinal axis of said tube to form a valve with the side of said tube during decanting.
5. A combination according to claim 4 further comprising a shaft extending along said tube and engaging said disk such that said disk slides along said shaft.
6. A combination according to claim 4 wherein said disk includes an upper outer edge and a lower outer edge and the transverse dimension between said upper outer edge and lower outer edge is greater than the internal diameter of said tube.
7. A combination according to claim 1 wherein the upper surface is curved.
8. A combination according to claim 7 wherein said upper surface is cylindrical.
9. A combination according to claim 1 wherein said disk is shaped such that its center of buoyancy is located above an upper surface of the disk.
10. A combination according to claim 9 wherein the specific gravity of said disk is such that its upper surface will lie just below an interface between plasma and red blood cells.
1 1.An article for use in preventing flow of a fluid from a tube, said article being generally disk shaped and having a raised edge portion such that the center of buoyancy of the article lies above an upper surface thereof.
12. A method for decanting a supernatant that has been separated from a fluid comprising the step of placing a floating separator disk in said fluid, separating said supernatant from said fluid, and decanting said supernatant wherein said separator disk automatically positions itself at or near the interface between said supernatant and the remaining components of said fluid.
13. A method according to claim 12 wherein said disk is adapted to restrict decant of said remaining components during decant of said supernatant.
14. A method according to claim 13 wherein said disk rotates during decant to a position wherein at least one edge of said disk engages a wall of a container having said fluid therein.
15. A method according to claim 13 wherein said disk forms a channel between an outer edge of said disk and an interior wall of a container having said fluid therein, said channel restricting flow of said remaining components during decant of said supernatant.
16. A method according to claim 13 wherein said disk assumes a position such that its upper surface is just below an interface between said supernatant and said remaining components.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US20015000P | 2000-04-28 | 2000-04-28 | |
US60/200,150 | 2000-04-28 | ||
PCT/US2001/011732 WO2001083068A1 (en) | 2000-04-28 | 2001-04-27 | Blood components separator disk |
Publications (2)
Publication Number | Publication Date |
---|---|
AU2001272085A1 true AU2001272085A1 (en) | 2002-01-31 |
AU2001272085B2 AU2001272085B2 (en) | 2006-03-02 |
Family
ID=22740538
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2001272085A Ceased AU2001272085B2 (en) | 2000-04-28 | 2001-04-27 | Blood components separator disk |
AU7208501A Pending AU7208501A (en) | 2000-04-28 | 2001-04-27 | Blood components separator disk |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU7208501A Pending AU7208501A (en) | 2000-04-28 | 2001-04-27 | Blood components separator disk |
Country Status (14)
Country | Link |
---|---|
US (8) | US7077273B2 (en) |
EP (1) | EP1289618B1 (en) |
JP (1) | JP4128007B2 (en) |
CN (1) | CN1309442C (en) |
AT (1) | ATE382408T1 (en) |
AU (2) | AU2001272085B2 (en) |
CA (1) | CA2407346C (en) |
CY (1) | CY1107189T1 (en) |
DE (1) | DE60132198T2 (en) |
DK (1) | DK1289618T3 (en) |
ES (1) | ES2298234T3 (en) |
HK (1) | HK1059059A1 (en) |
PT (1) | PT1289618E (en) |
WO (1) | WO2001083068A1 (en) |
Families Citing this family (60)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7947236B2 (en) | 1999-12-03 | 2011-05-24 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
AU2001272085B2 (en) * | 2000-04-28 | 2006-03-02 | Harvest Technologies Corporation | Blood components separator disk |
US20030205538A1 (en) | 2002-05-03 | 2003-11-06 | Randel Dorian | Methods and apparatus for isolating platelets from blood |
US7992725B2 (en) | 2002-05-03 | 2011-08-09 | Biomet Biologics, Llc | Buoy suspension fractionation system |
US7179391B2 (en) | 2002-05-24 | 2007-02-20 | Biomet Manufacturing Corp. | Apparatus and method for separating and concentrating fluids containing multiple components |
US7832566B2 (en) | 2002-05-24 | 2010-11-16 | Biomet Biologics, Llc | Method and apparatus for separating and concentrating a component from a multi-component material including macroparticles |
US20060278588A1 (en) | 2002-05-24 | 2006-12-14 | Woodell-May Jennifer E | Apparatus and method for separating and concentrating fluids containing multiple components |
US7845499B2 (en) | 2002-05-24 | 2010-12-07 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US7520402B2 (en) * | 2002-09-19 | 2009-04-21 | Harvest Technologies Corporation | Sterile disposable unit |
US7354515B2 (en) | 2004-02-23 | 2008-04-08 | Millennium Medical Technologies, Inc. | Fluid concentrator |
US7866485B2 (en) | 2005-02-07 | 2011-01-11 | Hanuman, Llc | Apparatus and method for preparing platelet rich plasma and concentrates thereof |
WO2006086201A2 (en) | 2005-02-07 | 2006-08-17 | Hanuman Llc | Platelet rich plasma concentrate apparatus and method |
EP2910258B1 (en) | 2005-02-07 | 2018-08-01 | Hanuman LLC | Platelet rich plasma concentrate apparatus |
US7771590B2 (en) | 2005-08-23 | 2010-08-10 | Biomet Manufacturing Corp. | Method and apparatus for collecting biological materials |
US8048297B2 (en) | 2005-08-23 | 2011-11-01 | Biomet Biologics, Llc | Method and apparatus for collecting biological materials |
US8567609B2 (en) | 2006-05-25 | 2013-10-29 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US7767087B2 (en) * | 2007-01-05 | 2010-08-03 | Wilson Kelce S | Floating filter holder |
US8328024B2 (en) | 2007-04-12 | 2012-12-11 | Hanuman, Llc | Buoy suspension fractionation system |
WO2008127639A1 (en) | 2007-04-12 | 2008-10-23 | Biomet Biologics, Llc | Buoy suspension fractionation system |
US20100260815A1 (en) * | 2007-06-22 | 2010-10-14 | Circle Biologics , LLC | Fluid concentrator, autologous concentrated body fluids, and uses thereof |
WO2009073232A1 (en) * | 2007-12-07 | 2009-06-11 | Harvest Technologies Corporation | Floating disk for separating blood components |
EP2620139B1 (en) | 2008-02-27 | 2016-07-20 | Biomet Biologics, LLC | Interleukin-1 receptor antagonist rich solutions |
US8337711B2 (en) | 2008-02-29 | 2012-12-25 | Biomet Biologics, Llc | System and process for separating a material |
US8012077B2 (en) | 2008-05-23 | 2011-09-06 | Biomet Biologics, Llc | Blood separating device |
MX339267B (en) | 2008-07-21 | 2016-05-18 | Becton Dickinson Co | Density phase separation device. |
CA2731156C (en) | 2008-07-21 | 2013-09-24 | Becton, Dickinson And Company | Density phase separation device |
AU2009274096B2 (en) | 2008-07-21 | 2012-08-02 | Becton, Dickinson And Company | Density phase separation device |
US8177072B2 (en) * | 2008-12-04 | 2012-05-15 | Thermogenesis Corp. | Apparatus and method for separating and isolating components of a biological fluid |
US8187475B2 (en) | 2009-03-06 | 2012-05-29 | Biomet Biologics, Llc | Method and apparatus for producing autologous thrombin |
US8313954B2 (en) | 2009-04-03 | 2012-11-20 | Biomet Biologics, Llc | All-in-one means of separating blood components |
AU2015204357B2 (en) * | 2009-05-15 | 2017-05-04 | Becton, Dickinson And Company | Density phase separation device |
PL2918344T3 (en) | 2009-05-15 | 2021-12-13 | Becton, Dickinson And Company | Density phase separation device |
US9272083B2 (en) | 2009-05-29 | 2016-03-01 | Endocellutions, Inc. | Apparatus and methods for aspirating and separating components of different densities from a physiological fluid containing cells |
US9011800B2 (en) | 2009-07-16 | 2015-04-21 | Biomet Biologics, Llc | Method and apparatus for separating biological materials |
US8313644B2 (en) * | 2010-01-13 | 2012-11-20 | OZOlab | Bottle with an integrated filtration assembly that is manually operated using a plunger |
DE102010003224B4 (en) * | 2010-03-24 | 2022-11-03 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Mixer for insertion in a rotor of a centrifuge |
US8591391B2 (en) | 2010-04-12 | 2013-11-26 | Biomet Biologics, Llc | Method and apparatus for separating a material |
US9555171B2 (en) | 2010-09-30 | 2017-01-31 | Depuy Mitek, Llc | Methods and devices for collecting separate components of whole blood |
US20120142514A1 (en) * | 2010-12-02 | 2012-06-07 | Medikan Co., Ltd. | Syringe for specific gravity distinction and fat tissue components separating method therewith |
US9011684B2 (en) | 2011-03-07 | 2015-04-21 | Spinesmith Holdings, Llc | Fluid concentrator with removable cartridge |
KR101170028B1 (en) * | 2011-12-20 | 2012-08-01 | (주) 레보메드 | Blood separating apparatus |
US9642956B2 (en) | 2012-08-27 | 2017-05-09 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
WO2014120797A1 (en) | 2013-01-29 | 2014-08-07 | Endocellutions, Inc. | Cell concentration devices and methods |
US10208095B2 (en) | 2013-03-15 | 2019-02-19 | Biomet Manufacturing, Llc | Methods for making cytokine compositions from tissues using non-centrifugal methods |
US9950035B2 (en) | 2013-03-15 | 2018-04-24 | Biomet Biologics, Llc | Methods and non-immunogenic compositions for treating inflammatory disorders |
US9895418B2 (en) | 2013-03-15 | 2018-02-20 | Biomet Biologics, Llc | Treatment of peripheral vascular disease using protein solutions |
US20140271589A1 (en) | 2013-03-15 | 2014-09-18 | Biomet Biologics, Llc | Treatment of collagen defects using protein solutions |
US10143725B2 (en) | 2013-03-15 | 2018-12-04 | Biomet Biologics, Llc | Treatment of pain using protein solutions |
US9382106B2 (en) * | 2013-07-19 | 2016-07-05 | Norman Werbner Information Services, Inc. | Liquid handling system with reduced exposure to air |
PL3212332T3 (en) | 2014-10-28 | 2021-08-09 | Arteriocyte Medical Systems, Inc. | Centrifuge tube comprising a floating buoy, and methods for using the same |
US9694359B2 (en) | 2014-11-13 | 2017-07-04 | Becton, Dickinson And Company | Mechanical separator for a biological fluid |
US9713810B2 (en) | 2015-03-30 | 2017-07-25 | Biomet Biologics, Llc | Cell washing plunger using centrifugal force |
US9757721B2 (en) | 2015-05-11 | 2017-09-12 | Biomet Biologics, Llc | Cell washing plunger using centrifugal force |
US10154745B2 (en) * | 2016-01-27 | 2018-12-18 | Daniel J. Noblitt | Methods and apparatus for liquid preservation |
US10384841B2 (en) | 2017-06-29 | 2019-08-20 | Norman Werbner Information Services, Inc. | Liquid extraction, storage, and dispensing system and method of use |
WO2020013981A1 (en) | 2018-07-09 | 2020-01-16 | Hanuman Pelican, Inc. | Apparatus and methods for processing blood |
WO2020013997A1 (en) | 2018-07-09 | 2020-01-16 | Hanuman Pelican, Inc. | Apparatus and methods for separating blood components |
WO2020154305A1 (en) | 2019-01-21 | 2020-07-30 | Eclipse Medcorp, Llc | Methods, systems and apparatus for separating components of a biological sample |
EP3890799A4 (en) | 2019-02-06 | 2022-10-26 | Hanuman Pelican, Inc. | Apparatus and methods for concentrating platelet-rich plasma |
KR20220091580A (en) | 2019-10-31 | 2022-06-30 | 이클립스 메드코프 엘엘씨 | Systems, methods and apparatus for isolating components of a sample |
Family Cites Families (98)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US593333A (en) * | 1897-11-09 | Device for separating liquids of different | ||
US280820A (en) * | 1883-07-10 | Milk-can | ||
US10140A (en) * | 1853-10-18 | Smproved life-preserving bucket | ||
US1818924A (en) * | 1928-09-04 | 1931-08-11 | Basmadjian Aronseag | Cover for pickles, etc. |
US3256977A (en) * | 1965-04-09 | 1966-06-21 | Pettersen Gunnar Nimrod | Filled packaging and dispensing container |
US3409165A (en) * | 1967-04-03 | 1968-11-05 | Olin Mathieson | Floating deck |
US3508653A (en) * | 1967-11-17 | 1970-04-28 | Charles M Coleman | Method and apparatus for fluid handling and separation |
US3618810A (en) * | 1969-10-21 | 1971-11-09 | Wilson Henry A | Shaving lather moistening and heating device |
US3647070A (en) | 1970-06-11 | 1972-03-07 | Technicon Corp | Method and apparatus for the provision of fluid interface barriers |
US3661265A (en) | 1970-07-27 | 1972-05-09 | Contemporary Research And Dev | Serum separator type container |
US3814248A (en) | 1971-09-07 | 1974-06-04 | Corning Glass Works | Method and apparatus for fluid collection and/or partitioning |
US3852194A (en) | 1972-12-11 | 1974-12-03 | Corning Glass Works | Apparatus and method for fluid collection and partitioning |
JPS517859B2 (en) | 1973-01-20 | 1976-03-11 | ||
US3814258A (en) | 1973-03-15 | 1974-06-04 | Dickinson And Co | Blood plasma separator with filter |
US4001122A (en) | 1973-08-22 | 1977-01-04 | Telan Corporation | Method and device for separating blood components |
FR2249265B1 (en) | 1973-10-25 | 1980-06-27 | Gele Pierre | |
US3957654A (en) | 1974-02-27 | 1976-05-18 | Becton, Dickinson And Company | Plasma separator with barrier to eject sealant |
US3894952A (en) | 1974-02-27 | 1975-07-15 | Becton Dickinson Co | Serum/plasma separator assembly having interface-seeking piston |
US3951801A (en) | 1974-02-27 | 1976-04-20 | Becton, Dickinson And Company | Serum/plasma separator-strut stop type |
US3897343A (en) | 1974-02-27 | 1975-07-29 | Becton Dickinson Co | Plasma separator-hydrostatic pressure type |
US3909419A (en) * | 1974-02-27 | 1975-09-30 | Becton Dickinson Co | Plasma separator with squeezed sealant |
US3931010A (en) | 1974-02-27 | 1976-01-06 | Becton, Dickinson And Company | Serum/plasma separators with centrifugal valves |
US3945928A (en) | 1974-02-27 | 1976-03-23 | Becton, Dickinson And Company | Serum/plasma separators with centrifugal valves |
US3887466A (en) | 1974-02-27 | 1975-06-03 | Becton Dickinson Co | Serum/plasma separator cannula fluid by-pass type centrifugal valve cannula seal |
US3894950A (en) | 1974-02-27 | 1975-07-15 | Becton Dickinson Co | Serum separator improvement with stretchable filter diaphragm |
US3920557A (en) | 1974-02-27 | 1975-11-18 | Becton Dickinson Co | Serum/plasma separator--beads-plus-adhesive type |
US3941699A (en) | 1974-02-27 | 1976-03-02 | Becton, Dickinson And Company | Plasma separator with centrifugal valve |
US3894951A (en) | 1974-02-27 | 1975-07-15 | Becton Dickinson Co | Serum/plasma separator; interface seeking piston; resilient apertures in lower diaphragm type |
US3935113A (en) | 1974-02-27 | 1976-01-27 | Becton, Dickinson And Company | Serum/plasma separator with centrifugal valve |
US3897337A (en) | 1974-02-27 | 1975-07-29 | Becton Dickinson Co | Plasma separator assembly having interface-seeking piston with centrifugal valve |
US3919085A (en) | 1974-02-27 | 1975-11-11 | Becton Dickinson Co | Plasma separator assembly |
US3929646A (en) * | 1974-07-22 | 1975-12-30 | Technicon Instr | Serum separator and fibrin filter |
US3931018A (en) | 1974-08-09 | 1976-01-06 | Becton, Dickinson And Company | Assembly for collection, separation and filtration of blood |
US3972812A (en) | 1975-05-08 | 1976-08-03 | Becton, Dickinson And Company | Blood serum separation filter disc |
US4083788A (en) * | 1975-11-19 | 1978-04-11 | Ferrara Louis T | Blood serum-isolation device |
US4180465A (en) | 1975-12-19 | 1979-12-25 | Sherwood Medical Industries Inc. | Fluid collection device with phase separation means |
US4055501A (en) | 1976-01-16 | 1977-10-25 | Sherwood Medical Industries Inc. | Fluid collection device with phase partitioning means |
US4088582A (en) | 1976-01-16 | 1978-05-09 | Sherwood Medical Industries Inc. | Blood phase separation means |
JPS52126613A (en) | 1976-04-16 | 1977-10-24 | Kubota Ltd | Heat-resisting cast alloy |
CA1074273A (en) | 1976-05-06 | 1980-03-25 | Sherwood Medical Industries Inc. | Phase separation device |
AT381466B (en) | 1977-03-16 | 1986-10-27 | Ballies Uwe | SEPARATING TUBES FOR CENTRIFUGAL SEPARATION |
SE7710076L (en) | 1977-09-08 | 1979-03-09 | Ericson Curt | BLOOD SAMPLING CONTAINER |
US4169060A (en) | 1977-10-25 | 1979-09-25 | Eastman Kodak Company | Blood-collecting and serum-dispensing device |
JPS5917386B2 (en) | 1979-03-23 | 1984-04-20 | テルモ株式会社 | Blood separation method and device |
SE416378B (en) | 1979-03-28 | 1980-12-22 | Johansson A S | SET ON SEPARATION OF BLOOD COMPONENTS FROM WHOLE BLOOD APPLICABLE BLOOD PASS SYSTEM FOR EXECUTIVE DEVICE SET |
US4310430A (en) | 1979-09-11 | 1982-01-12 | Terumo Corporation | α-Olefin-dialkylmaleate-based liquid separating agent |
US4279863A (en) * | 1979-09-12 | 1981-07-21 | Sherwood Medical Industries, Inc. | Reagent separator for a blood collection tube |
US4369117A (en) | 1980-05-12 | 1983-01-18 | American Hospital Supply Corporation | Serum separating method and apparatus |
JPS56168814A (en) | 1980-06-02 | 1981-12-25 | Terumo Corp | Barrier for separation of blood |
DE3101733C2 (en) * | 1981-01-21 | 1982-10-14 | Uwe Dr.Med. 2300 Kiel Ballies | Separating element in a separating tube for centrifugal separation |
US4707276A (en) | 1981-04-15 | 1987-11-17 | Sherwood Medical Company | Fluid collection device with phase partitioning means |
US4417981A (en) * | 1981-05-04 | 1983-11-29 | Becton, Dickinson And Company | Blood phase separator device |
US4443345A (en) | 1982-06-28 | 1984-04-17 | Wells John R | Serum preparator |
US4492634A (en) | 1982-09-28 | 1985-01-08 | Emde Medical Research | Pre-evacuated blood collection tube with anti-hemolysis baffle system and centrifugation propelled filtration disc and efficient serum-from cells separator |
US4487700A (en) | 1983-02-18 | 1984-12-11 | Technicon Instruments Corporation | Method and apparatus for separating lymphocytes from anticoagulated blood |
US4563332A (en) * | 1983-04-27 | 1986-01-07 | Icl Scientific, Inc. | Liquid sampling apparatus with retention means |
DE3343887A1 (en) | 1983-12-05 | 1985-06-13 | Walter Sarstedt Kunststoff-Spritzgußwerk, 5223 Nümbrecht | ARRANGEMENT FOR PLACING A SEPARATOR BETWEEN TWO PHASES IN A SAMPLE TUBE |
US4751001A (en) | 1984-09-24 | 1988-06-14 | Becton Dickinson And Company | Blood partitioning apparatus |
US5053134A (en) * | 1984-12-04 | 1991-10-01 | Becton Dickinson And Company | Lymphocyte collection tube |
IL74967A (en) | 1985-04-18 | 1988-10-31 | Assaf Pharmaceutical Ind | Separation of materials from a liquid dispersion by sedimentation |
SE448323B (en) | 1985-08-27 | 1987-02-09 | Ersson Nils Olof | PROCEDURE AND PROCEDURE TO SEPARATE SERUM OR PLASMA FROM BLOOD |
JPH0677014B2 (en) | 1987-08-14 | 1994-09-28 | テルモ株式会社 | Blood separation tube |
US4877520A (en) | 1987-10-08 | 1989-10-31 | Becton, Dickinson And Company | Device for separating the components of a liquid sample having higher and lower specific gravities |
US4818386A (en) | 1987-10-08 | 1989-04-04 | Becton, Dickinson And Company | Device for separating the components of a liquid sample having higher and lower specific gravities |
US4844818A (en) * | 1987-10-23 | 1989-07-04 | Becton Dickinson & Company | Method for separating the cellular components of blood samples |
US4989403A (en) | 1988-05-23 | 1991-02-05 | Sundstrand Corporation | Surge protected gas turbine engine for providing variable bleed air flow |
US4946601A (en) | 1988-08-22 | 1990-08-07 | Sherwood Medical Company | Blood serum separator tube |
US4946604A (en) * | 1988-11-23 | 1990-08-07 | Halliburton Company | Method for treating a well bore |
US4954264A (en) | 1989-02-02 | 1990-09-04 | Becton-Dickinson And Company | Apparatus for separating mononuclear cells from blood and method of manufacturing and using the same |
DE8910591U1 (en) | 1989-09-05 | 1989-12-21 | Walter Sarstedt Geräte und Verbrauchsmaterial für Medizin und Wissenschaft, 5223 Nümbrecht | Blood serum collection device |
AU6870091A (en) | 1989-11-08 | 1991-06-13 | Fmc Corporation | Combined centrifuge tube and porous selection means for separation and recovery of biological materials |
JPH0774772B2 (en) | 1990-12-31 | 1995-08-09 | エイ. レビン ロバート | Blood sampling assembly, target cell collection method and target component collection method |
US5269927A (en) | 1991-05-29 | 1993-12-14 | Sherwood Medical Company | Separation device for use in blood collection tubes |
US5316779A (en) * | 1991-09-16 | 1994-05-31 | Morey Booker W | Foam-limiting drinking cup and method |
IL100828A (en) | 1992-01-31 | 2002-05-23 | Novamed Ltd | Method and means for density gradient centrifugation |
US5282981A (en) | 1992-05-01 | 1994-02-01 | E. I. Du Pont De Nemours And Company | Flow restrictor-separation device |
US5389265A (en) | 1993-06-02 | 1995-02-14 | E. I. Du Pont De Nemours And Company | Phase-separation tube |
US5456885A (en) | 1993-07-12 | 1995-10-10 | Coleman; Charles M. | Fluid collection, separation and dispensing tube |
JPH07103969A (en) | 1993-08-13 | 1995-04-21 | Niigata Kako Kk | Blood separation member and blood collecting tube for blood separation |
US5533518A (en) | 1994-04-22 | 1996-07-09 | Becton, Dickinson And Company | Blood collection assembly including mechanical phase separating insert |
US5577513A (en) | 1994-08-31 | 1996-11-26 | Activated Cell Therapy, Inc. | Centrifugation syringe, system and method |
US5840502A (en) | 1994-08-31 | 1998-11-24 | Activated Cell Therapy, Inc. | Methods for enriching specific cell-types by density gradient centrifugation |
BR9509860A (en) * | 1994-12-02 | 1997-09-30 | Squibb & Sons Inc | Centrifuge reagent delivery system |
US5560830A (en) | 1994-12-13 | 1996-10-01 | Coleman; Charles M. | Separator float and tubular body for blood collection and separation and method of use thereof |
US5707331A (en) * | 1995-05-05 | 1998-01-13 | John R. Wells | Automatic multiple-decanting centrifuge |
US5632905A (en) * | 1995-08-07 | 1997-05-27 | Haynes; John L. | Method and apparatus for separating formed and unformed components |
US5736033A (en) * | 1995-12-13 | 1998-04-07 | Coleman; Charles M. | Separator float for blood collection tubes with water swellable material |
US5707876A (en) * | 1996-03-25 | 1998-01-13 | Stephen C. Wardlaw | Method and apparatus for harvesting constituent layers from a centrifuged material mixture |
US5785925A (en) | 1996-08-29 | 1998-07-28 | Saigene Corporation | Centrifuge tube phase separation plug |
US5889584A (en) | 1997-03-10 | 1999-03-30 | Robert A. Levine | Assembly for rapid measurement of cell layers |
US5860937A (en) | 1997-04-30 | 1999-01-19 | Becton, Dickinson & Company | Evacuated sample collection tube with aqueous additive |
US5918622A (en) | 1997-07-01 | 1999-07-06 | Bermad | Separation valve |
US6406671B1 (en) * | 1998-12-05 | 2002-06-18 | Becton, Dickinson And Company | Device and method for separating components of a fluid sample |
US6280400B1 (en) | 1998-12-05 | 2001-08-28 | Becton Dickinson And Company | Device and method for separating component of a liquid sample |
US6409528B1 (en) | 1999-12-06 | 2002-06-25 | Becton, Dickinson And Company | Device and method for collecting, preparation and stabilizing a sample |
JP3619933B2 (en) | 2000-03-02 | 2005-02-16 | アークレイ株式会社 | Centrifuge container |
US20020042335A1 (en) * | 2000-04-18 | 2002-04-11 | Anderson Norman G. | Method and apparatus for making density gradients |
AU2001272085B2 (en) * | 2000-04-28 | 2006-03-02 | Harvest Technologies Corporation | Blood components separator disk |
-
2001
- 2001-04-27 AU AU2001272085A patent/AU2001272085B2/en not_active Ceased
- 2001-04-27 US US10/019,680 patent/US7077273B2/en not_active Expired - Lifetime
- 2001-04-27 ES ES01930473T patent/ES2298234T3/en not_active Expired - Lifetime
- 2001-04-27 DE DE60132198T patent/DE60132198T2/en not_active Expired - Lifetime
- 2001-04-27 DK DK01930473T patent/DK1289618T3/en active
- 2001-04-27 WO PCT/US2001/011732 patent/WO2001083068A1/en active IP Right Grant
- 2001-04-27 CA CA002407346A patent/CA2407346C/en not_active Expired - Fee Related
- 2001-04-27 EP EP01930473A patent/EP1289618B1/en not_active Expired - Lifetime
- 2001-04-27 AT AT01930473T patent/ATE382408T1/en active
- 2001-04-27 JP JP2001579934A patent/JP4128007B2/en not_active Expired - Lifetime
- 2001-04-27 AU AU7208501A patent/AU7208501A/en active Pending
- 2001-04-27 PT PT01930473T patent/PT1289618E/en unknown
- 2001-04-27 CN CNB018087442A patent/CN1309442C/en not_active Expired - Lifetime
-
2004
- 2004-03-10 HK HK04101761A patent/HK1059059A1/en not_active IP Right Cessation
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2005
- 2005-08-19 US US11/206,869 patent/US7547272B2/en not_active Ceased
-
2008
- 2008-02-13 CY CY20081100164T patent/CY1107189T1/en unknown
-
2009
- 2009-05-15 US US12/453,577 patent/US20090283524A1/en not_active Abandoned
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2011
- 2011-06-15 US US13/161,239 patent/USRE43547E1/en not_active Expired - Lifetime
-
2012
- 2012-11-19 US US13/680,350 patent/US20130079212A1/en not_active Abandoned
-
2014
- 2014-01-22 US US14/160,809 patent/US9393575B2/en not_active Expired - Lifetime
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2015
- 2015-06-25 US US14/750,412 patent/US9393576B2/en not_active Expired - Lifetime
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2016
- 2016-07-11 US US15/207,009 patent/US9656274B2/en not_active Expired - Lifetime
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