CA2405960A1 - Detection de flavivirus et analyse de quantification - Google Patents

Detection de flavivirus et analyse de quantification Download PDF

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Publication number
CA2405960A1
CA2405960A1 CA002405960A CA2405960A CA2405960A1 CA 2405960 A1 CA2405960 A1 CA 2405960A1 CA 002405960 A CA002405960 A CA 002405960A CA 2405960 A CA2405960 A CA 2405960A CA 2405960 A1 CA2405960 A1 CA 2405960A1
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CA
Canada
Prior art keywords
tcc
caa
cat
tct
gct
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002405960A
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English (en)
Inventor
Huo-Shu H. Houng
Niranjan Kanesa-Thasan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Army
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Individual
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Filing date
Publication date
Priority claimed from US09/551,161 external-priority patent/US6793488B1/en
Application filed by Individual filed Critical Individual
Publication of CA2405960A1 publication Critical patent/CA2405960A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Des sondes d'ADN spécifiques fluorescentes et des paires d'amorces flanquantes sont conçues sur la base des informations de séquence trouvées dans la région conservée terminale 3' non codante de flavivirus, par ex. les nucléotides 10653-10678 du virus de la dengue. L'amplification en chaîne par polymérase (PCR) fluorogène employant ces amorces et sondes produit des résultats qui permettent l'identification spécifique de flavivirus. Les analyses peuvent être tant qualitatives que quantitatives. Des conditions optimales d'analyse avec bruit de fond nul qui permettent la détection de faibles niveaux de flavivirus dans des spécimens cliniques sont également décrites. Spécifiquement, des virus de dengue isolés dans différentes régions géographiques peuvent être universellement détectés et identifiés par l'analyse fondée sur la RT-PCR fluorogène décrite. Ladite analyse RT-PCR fluorogène a permis de détecter facilement une virémie dans des sérums prélevés chez des patients atteints de la dengue.
CA002405960A 2000-04-14 2000-10-19 Detection de flavivirus et analyse de quantification Abandoned CA2405960A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/551,161 2000-04-14
US09/551,161 US6793488B1 (en) 1999-04-16 2000-04-14 Flavivirus detection and quantification assay
PCT/US2000/028961 WO2001079546A2 (fr) 2000-04-14 2000-10-19 Detection de flavivirus et analyse de quantification

Publications (1)

Publication Number Publication Date
CA2405960A1 true CA2405960A1 (fr) 2001-10-25

Family

ID=24200105

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002405960A Abandoned CA2405960A1 (fr) 2000-04-14 2000-10-19 Detection de flavivirus et analyse de quantification

Country Status (5)

Country Link
EP (1) EP1276898A1 (fr)
AU (1) AU2001212172A1 (fr)
BR (1) BR0017215A (fr)
CA (1) CA2405960A1 (fr)
WO (1) WO2001079546A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104342498A (zh) * 2013-07-30 2015-02-11 中国疾病预防控制中心病毒病预防控制所 库蚊黄病毒实时荧光定量rt-pcr检测方法及试剂盒

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7927840B2 (en) 2006-09-11 2011-04-19 Gen Probe Incorporated Method for detecting West Nile Virus nucleic acids in the 3′ non-coding region
AU2008200221B2 (en) * 2002-10-16 2012-04-05 Gen-Probe Incorporated Compositions and methods for detecting west nile virus
CA2501946C (fr) * 2002-10-16 2014-12-23 Gen-Probe Incorporated Compositions et methodes de detection du virus du nil occidental
WO2013080307A1 (fr) * 2011-11-29 2013-06-06 株式会社 東芝 Jeu d'amorces pour amplifier les virus transmis par les moustiques, kit de dosage pour détecter les virus transmis par les moustiques, et procédé de détection faisant appel audit jeu d'amorces et audit kit de dosage
CN104357582B (zh) * 2014-10-31 2017-02-15 中国检验检疫科学研究院 库京病毒的实时荧光rt‑pcr检测试剂盒和方法
CN107893128A (zh) * 2017-11-09 2018-04-10 贵州医科大学 同时检测10种虫媒病毒的引物对组合、探针及应用
CN110846441B (zh) * 2019-12-20 2023-04-11 浙江省淡水水产研究所 检测沼虾黄病毒-1的特异性引物、探针及快速检测试剂盒

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5939254A (en) * 1997-04-28 1999-08-17 University Of Massachusetts Methods and reagents for rapid diagnosis of dengue virus infection
US5968732A (en) * 1997-12-31 1999-10-19 Akzo Nobel, N.V. Isothermal transcription based assay for the detection and genotyping of dengue virus

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104342498A (zh) * 2013-07-30 2015-02-11 中国疾病预防控制中心病毒病预防控制所 库蚊黄病毒实时荧光定量rt-pcr检测方法及试剂盒

Also Published As

Publication number Publication date
AU2001212172A1 (en) 2001-10-30
BR0017215A (pt) 2004-02-10
EP1276898A1 (fr) 2003-01-22
WO2001079546A2 (fr) 2001-10-25
WO2001079546A3 (fr) 2003-06-05

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