CA2401961A1 - Cultured protein hydrolysate - Google Patents

Cultured protein hydrolysate Download PDF

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Publication number
CA2401961A1
CA2401961A1 CA002401961A CA2401961A CA2401961A1 CA 2401961 A1 CA2401961 A1 CA 2401961A1 CA 002401961 A CA002401961 A CA 002401961A CA 2401961 A CA2401961 A CA 2401961A CA 2401961 A1 CA2401961 A1 CA 2401961A1
Authority
CA
Canada
Prior art keywords
process according
lactic acid
acid bacteria
enzyme
lactobacillus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002401961A
Other languages
French (fr)
Other versions
CA2401961C (en
Inventor
Daniel Jaeger
Cristina Hering-Giovanola
Michael Affolter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nestec SA
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2401961A1 publication Critical patent/CA2401961A1/en
Application granted granted Critical
Publication of CA2401961C publication Critical patent/CA2401961C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/24Synthetic spices, flavouring agents or condiments prepared by fermentation
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/30Working-up of proteins for foodstuffs by hydrolysis
    • A23J3/32Working-up of proteins for foodstuffs by hydrolysis using chemical agents
    • A23J3/34Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/50Soya sauce
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • C12P13/14Glutamic acid; Glutamine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/06Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225Lactobacillus

Abstract

This invention relates to a process for the production of a cultured savory base by hydrolyzing a protein-containing material using a combination of at least one enzyme with at least one thermotolerant lactic acid bacteria strain selected for its ability to provide a glutaminase activity. The invention also relates to an isolated strain of thermotolerant lactic acid bacteria strains selected for their ability to provide a glutaminase activity and their use for preparing a seasoning or flavor for culinary or petfood products.

Claims (22)

1. A process for the preparation of a cultured savoury base, which comprises hydrolysing a protein-containing material using a combination of at least one enzyme with at least one thermotolerant lactic acid bacteria strain selected for its ability to provide a glutaminase activity.
2. A process according to claim 1, wherein the hydrolysate is further processed downstream by means of thermal inactivation, filtration and/or centrifugation to produce a raw sauce.
3. A process according to claim 1 or 2, wherein the protein containing material is from plant or animal origin.
4. A process according to claim 3, wherein the protein containing material is wheat gluten, rice protein, soya, corn gluten, sunflower presscake, milk proteins and animal proteins.
5. A process according to one of claims 1 to 4, wherein the ratio enzyme :
lactic acid bacteria strain is of about 1:1 to 4:1 based on % (w/w) addition of the ingredients.
6. A process according to one of claims 1 to 5, in which the enzyme is an exo-or endo- protease, deaminase, carbohydrase or amyloglucosidase.
7. A process according to claim 6, in which the enzyme is Flavorzyme®, Alcalase®, Dextrozyme® or AMG® or glutaminase C200®.
8. A process according to one of claims 1 to 7, in which the enzyme is used in an amount of at least 0.2 % (protease) or 0.05% (carbohydrase) by weight based on the total reaction.
9. A process according to one of claims 1 to 8, in which the thermotolerant lactic acid bacteria is selected from the group consisting of facultative heterofermentative Lactobacilli, Lactobacillus rhamnosus, Lactobacillus delbrucki, Lactobacillus casei, Lactobacillus paracasei or Lactobacillus sp.
10. A process according to one of claims 1 to 9, wherein the lactic acid bacteria is Lactobacillus rhamnosus (NCC 858) CNCM I-2433.
11. A process according to one of claims 1 to 10, in which the lactic acid bacteria is added in the form of a starter having a titer of about 1.10 8- 1.10 11 cfu/g, in an amount of at least 0.1 to 0.5 % (w/w)
12. A process according to one of claims 1 to 11, wherein the hydrolysis is carried out for a time sufficient for the lactic bacteria strain to grow by 2-3 log, or to create 1-4% mono-sodium glutamate (MSG) in the hydrolysate, or to have a degree of hydrolysis (DH) of at least 20%.
13. A process according to one of claims 1 to 12, in which the hydrolysis is carried out at 43°C - 48°C, at pH 5- 6 for a time of at least 12 hours.
14. A process according to one of claims 1 to 13, wherein a slurry containing
15 to 30% of the protein containing material and 65 to 80% of water, is hydrolyzed using 1 to 4% of at least one enzyme such as Flavorzyme® combined with 0.5 to 2% of a lactic acid bacteria starter having a titer of about 1.10 5-1.10 6 cfu/g, in an amount of at least 0.1 to 0.5 % (w/w).
15. The use of a savory base prepared according to one of claims 1 to 14, as body giver in process flavour.
16. The use of a savory base prepared according to one of claims 1 to 14, for the preparation of seasonings, flavours and culinary or petfood products.
17. An isolated thermotolerant lactic acid bacteria strain selected for its ability to provide a glutaminase activity.
18. An isolated strain according to claim 17, which is selected from the group consisting of facultative heterofermentative Lactobacilli, Lactobacillus delbrueckii, Lactobacillus casei, Lactobacillus paracasei and Lactobacillus rhamnosus .
19. An isolated strain according to claim 17 or 18, which is Lactobacillus rhamnosus (NCC 858) CNCM I-2433.
20. The use of an isolated strain of lactic acid bacteria according one of claims 17 to 19, as process micro-organism for the preparation of seasonings, flavours and culinary or petfood products.
21. The use of an isolated strain according to claim 20, in combination with at least one enzyme.
22. The use according to claims 20 or 21, wherein the ratio enzyme : lactic acid bacteria strain is of about 1:1 to 4:1 based on % (w/w) addition of the ingredients.
CA2401961A 2000-04-07 2001-04-03 Cultured protein hydrolysate Expired - Fee Related CA2401961C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00201274 2000-04-07
EP00201274.8 2000-04-07
PCT/EP2001/003807 WO2001076391A1 (en) 2000-04-07 2001-04-03 Cultured protein hydrolysate

Publications (2)

Publication Number Publication Date
CA2401961A1 true CA2401961A1 (en) 2001-10-18
CA2401961C CA2401961C (en) 2011-02-22

Family

ID=8171320

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2401961A Expired - Fee Related CA2401961C (en) 2000-04-07 2001-04-03 Cultured protein hydrolysate

Country Status (15)

Country Link
US (1) US6838100B2 (en)
EP (1) EP1274318B1 (en)
JP (1) JP2003529383A (en)
KR (1) KR20030005268A (en)
CN (1) CN1245894C (en)
AT (1) ATE516714T1 (en)
AU (1) AU2001244229A1 (en)
BR (1) BR0109856B1 (en)
CA (1) CA2401961C (en)
ES (1) ES2368990T3 (en)
IL (1) IL151338A0 (en)
NZ (1) NZ520886A (en)
PL (1) PL358431A1 (en)
PT (1) PT1274318E (en)
WO (1) WO2001076391A1 (en)

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AR047658A1 (en) 2004-02-03 2006-02-01 Cargill Inc CONCENTRATE OF PROTEINS AND WATER CURRENT WITH HYDROSOLUBBLE CARBOHYDRATES
JP2006094708A (en) * 2004-09-28 2006-04-13 Marukome Kk Soymilk food, and method for producing the same
KR101221239B1 (en) * 2005-07-07 2013-01-11 (주)바이오벤 Lactic acid bacteria culture of Mung bean and the preparation method of the same, and the cosmetic composition comprising the same
WO2007039253A1 (en) 2005-09-30 2007-04-12 Oterap Holding B.V. Hydrolysis of vegetable proteins
GB2452953B (en) * 2007-09-20 2010-03-10 Kraft Foods R & D Inc Coffee Composition
EP2042043A1 (en) * 2007-09-26 2009-04-01 Nestec S.A. A shelf-stable taste enhancing cultured savoury base and a process for its preparation
CN101513248B (en) * 2008-02-19 2013-05-15 安琪酵母股份有限公司 Yeast extract containing inosinic acid disodium salt and guanylic acid disodium and method for preparing same
CN106820065B (en) * 2008-03-17 2021-06-22 奇华顿股份有限公司 Enzymatic process
BRPI0914871B1 (en) * 2008-06-20 2018-12-11 Givaudan Sa salt raising ingredient, its process of forming from spinach plant material hydrolyzate, food product, flavoring composition for food products and method of providing a food product with increased salinity
RU2527176C2 (en) 2008-12-09 2014-08-27 Нестек С.А. Capsule for beverage preparation by centrifugation method and beverage preparation device
CN104664307A (en) * 2009-02-18 2015-06-03 雀巢产品技术援助有限公司 Base stock, product containing same and preparation method and application of base stock
ES2593154T3 (en) 2009-02-18 2016-12-05 Nestec S.A. Base, products containing it, preparation methods and uses thereof
WO2010108542A1 (en) * 2009-03-25 2010-09-30 Nestec S.A. A natural taste enhancing savoury base and a process for its preparation
KR101138076B1 (en) 2009-12-29 2012-04-24 샘표식품 주식회사 Method for producing low molecular soy peptide improving kokumi taste
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US8939388B1 (en) 2010-09-27 2015-01-27 ZoomEssence, Inc. Methods and apparatus for low heat spray drying
US9332776B1 (en) 2010-09-27 2016-05-10 ZoomEssence, Inc. Methods and apparatus for low heat spray drying
KR101303951B1 (en) * 2010-11-05 2013-09-05 태경농산주식회사 Preparation method for Gluten Hydrolysate
KR101284264B1 (en) * 2010-12-29 2013-07-08 주식회사농심 Preparation Method of Rice protein hydrolysate
KR101295633B1 (en) * 2010-12-29 2013-08-12 주식회사농심 Manufacturing method of Rice protein plain including high level of Branched chain amino acids
CN102524517A (en) * 2010-12-30 2012-07-04 保定味群食品科技股份有限公司 Method for preparing gluten amino acid
UA111078C2 (en) * 2011-05-03 2016-03-25 Нестек С.А. Hydrolysate of a protein substrate and a method for obtaining thereof
JP6019528B2 (en) * 2012-06-05 2016-11-02 池田食研株式会社 Method for producing fermented seasoning
WO2014209912A1 (en) * 2013-06-27 2014-12-31 Starbucks Corporation D/B/A Starbucks Coffee Company Biopreservation methods for beverages and other foods
WO2015131226A1 (en) * 2014-03-05 2015-09-11 Shoalhaven Starches Pty Ltd Functional ingredients from enzyme-modified wheat proteins
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Also Published As

Publication number Publication date
IL151338A0 (en) 2003-04-10
US20030113403A1 (en) 2003-06-19
KR20030005268A (en) 2003-01-17
CN1419416A (en) 2003-05-21
NZ520886A (en) 2004-04-30
PT1274318E (en) 2011-09-07
ES2368990T3 (en) 2011-11-24
CA2401961C (en) 2011-02-22
JP2003529383A (en) 2003-10-07
ATE516714T1 (en) 2011-08-15
PL358431A1 (en) 2004-08-09
AU2001244229A1 (en) 2001-10-23
EP1274318A1 (en) 2003-01-15
BR0109856B1 (en) 2014-04-29
EP1274318B1 (en) 2011-07-20
BR0109856A (en) 2003-06-03
US6838100B2 (en) 2005-01-04
CN1245894C (en) 2006-03-22
WO2001076391A1 (en) 2001-10-18

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Effective date: 20190403