CA2400562C - Hybrid expression of neisserial proteins - Google Patents

Hybrid expression of neisserial proteins Download PDF

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CA2400562C
CA2400562C CA2400562A CA2400562A CA2400562C CA 2400562 C CA2400562 C CA 2400562C CA 2400562 A CA2400562 A CA 2400562A CA 2400562 A CA2400562 A CA 2400562A CA 2400562 C CA2400562 C CA 2400562C
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CA2400562A1 (en
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Maria Beatrice Arico
Maurizio Comanducci
Cesira Galeotti
Vega Masignani
Marzia Monica Giuliani
Mariagrazia Pizza
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GSK Vaccines SRL
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Chiron SRL
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Abstract

Two or more Neisserial proteins (e.g. A and B) are expressed as a single hybrid protein which can be represented simply by the formula NH2-A-B-COOH.

Description

HYBRID EXPRESSION OF NEISSERIAL PROTEINS
All documents cited herein are incorporated by reference in their entirety.
TECHNICAL FIELD

This invention is in the field of protein expression. In particular, it relates to the heterologous expression of proteins from Neisseria (e.g. N.gonorrhoeae or, preferably, N.meningitidis).
BACKGROUND ART

International patent applications W099/24578, W099/36544, W099/57280 and W000/22430 disclose proteins from Neisseria meningitidis and Neisseria gonorrhoeae.
These proteins are typically described as being expressed in E.coli (i.e.
heterologous expression) as either N-terminal GST-fusions or C-terminal His-tag fusions, although other expression systems, including expression in native Neisseria, are also disclosed.

It is an object of the present invention to provide alternative and improved approaches for the heterologous expression of these proteins. These approaches will typically affect the level of expression, the ease of purification, the cellular localisation of expression, and/or the immunological properties of the expressed protein.

DISCLOSURE OF THE INVENTION

In accordance with the invention, two or more (e.g. 3, 4, 5, 6 or more) proteins of the invention are expressed as a single hybrid protein. It is preferred that no non-Neisserial fusion partner (e.g. GST or poly-His) is used.

This offers two advantages. Firstly, a protein that may be unstable or poorly expressed on its own can be assisted by adding a suitable hybrid partner that overcomes the problem.
Secondly, commercial manufacture is simplified - only one expression and purification need be employed in order to produce two separately-useful proteins.

Thus the invention provides a method for the simultaneous heterologous expression of two or more proteins of the invention, in which said two or more proteins of the invention are fused (i.e. they are translated as a single polypeptide chain).

The method will typically involve the steps of. obtaining a first nucleic acid encoding a first protein of the invention; obtaining a second nucleic acid encoding a second protein of the invention; ligating the first and second nucleic acids. The resulting nucleic acid may be inserted into an expression vector, or may already be part of an expression vector.

Where just two proteins are joined, the hybrid protein can be represented simply by the formula NH2-A-B-COOH. A and B can each be selected from any Neisserial proteins, and in particular those represented by SEQ#s 1-4326. The method is well suited to the expression of proteins orfl, orf4, orf25, orf40, Orf46/46.1, orf83, 233, 287, 292L, 564, 687, 741, 907, 919, 953, 961 and 983.

The 42 hybrids indicated by `X' in the following table of form NH2-A-B-COOH
are preferred:

.1 A', B-+ ORF46.1 287, 741 919 "953 961 983 Preferred proteins to be expressed as hybrids are thus ORF46.1, 287, 741, 919, 953, 961 and 983. These may be used in their essentially full-length form, or poly-glycine deletions (AG) forms may be used (e.g. AG-287, AGTbp2, AG741, AG983 etc.), or truncated forms may be used (e.g. Al-287, A2-287 etc.), or domain-deleted versions may be used (e.g.
287B, 287C, 287BC, ORF461-433, ORF46433-608, ORF46, 961c etc.) and so on.

Particularly preferred are: (a) a hybrid protein comprising 919 and 287; (b) a hybrid protein comprising 953 and 287; (c) a hybrid protein comprising 287 and ORF46.1; (d) a hybrid protein comprising ORF1 and ORF46.1; (e) a hybrid protein comprising 919 and ORF46.1;
(f) a hybrid protein comprising ORF46.1 and 919; (g) a hybrid protein comprising ORF46.1, 287 and 919; (h) a hybrid protein comprising 919 and 519; and (i) a hybrid protein comprising ORF97 and 225.

Further embodiments are shown in the drawings and include AG287-919, AG287-953, AG287-961, AG983-ORF46.1, AG983-741, AG983-961, AG983-961C, AG741-961, AG741-961C, AG741-983, AG741-ORF46.1, ORF46.1-741, ORF46.1-961, ORF46.1-961C, 961-ORF46.1, 961-741, 961-983, 961C-ORF46.1, 961C-741, 961C-983, 961CL-ORF46.1, 961CL-741, and 961CL-983.

Where 287 is used, it is preferably at the C-terminal end of a hybrid; if it is to be used at the N-terminus, if is preferred to use a AG form of 287 is used (e.g. as the N-terminus of a hybrid with ORF46.1, 919, 953 or 961).

Where 287 is used, this is preferably from strain 2996 or from strain 394/98.

Where 961 is used, this is preferably at the N-terminus. Domain forms of 961 may be used.
Alignments of polymorphic forms of ORF46, 287, 919 and 953 are disclosed in W000/66741. Any of these polymorphs can be used according to the present invention.

Preferably, the constituent proteins (A and B) in a hybrid protein according to the invention will be from the same strain.

The fused proteins in the hybrid may be joined directly, or may be joined via a linker peptide e.g. via a poly-glycine linker (i.e. G, where n = 3, 4, 5, 6, 7, 8, 9, 10 or more) or via a short peptide sequence which facilitates cloning. It is evidently preferred not to join a AG protein to the C-terminus of a poly-glycine linker.

The fused proteins may lack native leader peptides or may include the leader peptide sequence of the N-terminal fusion partner.

Host It is preferred to utilise a heterologous host. The heterologous host may be prokaryotic or eukaryotic. It is preferably E.coli, but other suitable hosts include Bacillus subtilis, Vibrio cholerae, Salmonella typhi, Salmonenna typhimurium, Neisseria meningitidis, Neisseria gonorrhoeae, Neisseria lactamica, Neisseria cinerea, Mycobateria (e.g. M.
tuberculosis), yeast etc.

Vectors, hosts etc.

As well as the methods described above, the invention provides (a) nucleic acid and vectors useful in these methods (b) host cells containing said vectors (c) proteins expressed or expressable by the methods (d) compositions comprising these proteins, which may be suitable as vaccines, for instance, or as diagnostic reagents, or as immunogenic compositions (e) these compositions for use as medicaments (e.g. as vaccines) or as diagnostic reagents (f) the use of these compositions in the manufacture of (1) a medicament for treating or preventing infection due to Neisserial bacteria (2) a diagnostic reagent for detecting the presence of Neisserial bacteria or of antibodies raised against Neisserial bacteria, and/or (3) a reagent which can raise antibodies against Neisserial bacteria and (g) a method of treating a patient, comprising administering to the patient a therapeutically effective amount of these compositions.

Sequences The invention also provides a protein or a nucleic acid having any of the sequences set out in the following examples. It also provides proteins and nucleic acid having sequence identity to these. As described above, the degree of `sequence identity' is preferably greater than 50% (eg. 60%, 70%, 80%, 90%, 95%, 99% or more).

Nomenclature herein The 2166 protein sequences disclosed in W099/24578, W099/36544 and W099/57280 are referred to herein by the following SEQ# numbers:

Application Protein sequences SEQ# herein W099/24578 Even SEQ IDs 2-892 SEQ#s 1-446 W099/36544 Even SEQ IDs 2-90 SEQ#s 447-491 Even SEQ IDs 2-3020 SEQ#s 492-2001 W099/57280 Even SEQ IDs 3040-3114 SEQ#s 2002-2039 SEQ IDs 3115-3241 SEQ#s 2040-2166 In addition to this SEQ# numbering, the naming conventions used in W099/24578, W099/36544 and W099/57280 are also used (e.g. `ORF4', `ORF40', `ORF40-1' etc.
as used in W099/24578 and W099/36544; 'm919', `g919' and 'a919' etc. as used in W099/57280).

The 2160 proteins NMB 0 0 01 to NMB216 0 from Tettelin et al. [Science (2000) 287:1809-1815] are referred to herein as SEQ#s 2167-4326 [see also W000/66791].

The term `protein of the invention' as used herein refers to a protein comprising:
(a) one of sequences SEQ#s 1-4326; or (b) a sequence having sequence identity to one of SEQ#s 1-4326; or (c) a fragment of one of SEQ#s 1-4326.
The degree of `sequence identity' referred to in (b) is preferably greater than 50% (eg. 60%, 70%, 80%, 90%, 95%, 99% or more). This includes mutants and allelic variants [e.g. see W000/66741]. Identity is preferably determined by the Smith-Waterman homology search algorithm as implemented in the MPSRCH program (Oxford Molecular), using an affine gap search with parameters gap open penalty=12 and gap extension penalty=l.
Typically, 50%
identity or more between two proteins is considered to be an indication of functional equivalence.

The `fragment' referred to in (c) should comprise at least n consecutive amino acids from one of SEQ#s 1-4326 and, depending on the particular sequence, n is 7 or more (eg. 8, 10, 12, 14, 16, 18, 20, 25, 30, 35, 40, 50, 60, 70, 80, 90, 100 or more).
Preferably the fragment comprises an epitope from one of SEQ#s 1-4326. Preferred fragments are those disclosed in W 000/71574 and WOO 1/04316.

Preferred proteins of the invention are found in N.meningitidis serogroup B.

Preferred proteins for use according to the invention are those of serogroup B
N.meningitidis strain 2996 or strain 394/98 (a New Zealand strain). Unless otherwise stated, proteins mentioned herein are from N.meningitidis strain 2996. It will be appreciated, however, that the invention is not in general limited by strain. References to a particular protein (e.g. `287', `919' etc.) may be taken to include that protein from any strain.

It will be appreciated that references to "nucleic acid" includes DNA and RNA, and also their analogues, such as those containing modified backbones, and also peptide nucleic acids (PNA) etc.

BRIEF DESCRIPTION OF DRAWINGS

Figures 1 to 26 show hybrid proteins according to the invention.
MODES FOR CARRYING OUT THE INVENTION

Example I - hybrids of ORF46 The complete ORF46 protein from N.meningitidis (serogroup B, strain 2996) has the following sequence:
601 KRPRYRSK*

The leader peptide is underlined.

The sequences of ORF46 from other strains can be found in W000/66741.

ORF46 has been fused at its C-terminus and N-terminus with 287, 919, and ORF1.
The hybrid proteins were generally insoluble, but gave some good ELISA and bactericidal results (against the homologous 2996 strain):

Protein ELISA Bactericidal Ab Orfl-Orf46.1-His 850 256 919-Orf46. 1 -His 12900 512 919-287-Orf46-His n.d. n.d.
Orf46.1-287His 150 8192 Orf46.1-919His 2800 2048 Orf46.1-287-919His 3200 16384 For comparison, `triple' hybrids of ORF46.1, 287 (either as a GST fusion, or in AG287 form) and 919 were constructed and tested against various strains (including the homologous 2996 strain) versus a simple mixture of the three antigens. FCA was used as adjuvant:

Mixture 8192 256 512 1024 >2048 >2048 ORF46.1-287-919his 16384 256 4096 8192 8192 8192 AG287-919-ORF46.lhis 8192 64 4096 8192 8192 16384 AG287-ORF46.1-919his 4096 128 256 8192 512 1024 Again, the hybrids show equivalent or superior immunological activity.

Hybrids of two proteins (strain 2996) were compared to the individual proteins against various heterologous strains:

1000 MC58 F6124 (MenA) ORF46.1-His <4 4096 <4 ORF1-His 8 256 128 ORF1-0RF46.1-His 1024 512 1024 Again, the hybrid shows equivalent or superior immunological activity.
Example 2 - hybrids of AG287 The deletion of the (Gly)6 sequence in 287 was found to have a dramatic effect on protein expression. The protein lacking the N-terminal amino acids up to GGGGGG is called `AG287'. In strain MC58, its basic sequence (leader peptide underlined) is:

SPDVKS ADTLSKPAAP VVSEKETEAK EDAPQAGSQG QGAPSAQGSQ DMAAVSEENT
GNGGAVTADN PKNEDEVAQN DMPQNAAGTD SSTPNHTPDP NMLAGNMENQ ATDAGESSQP
ANQPDMANAA DGMQGDDPSA GGQNAGNTAA QGANQAGNNQ AAGSSDPIPA SNPAPANGGS
NFGRVDLANG VLIDGPSQNI TLTHCKGDSC SGNNFLDEEV QLKSEFEKLS DADKISNYKK
DGKNDKFVGL VADSVQMKGI NQYIIFYKPK PTSFARFRRS ARSRRSLPAE MPLIPVNQAD
TLIVDGEAVS LTGHSGNIFA PEGNYRYLTY GAEKLPGGSY ALRVQGEPAK GEMLAGAAVY
NGEVLHFHTE NGRPYPTRGR FAAKVDFGSK SVDGIIDSGD DLHMGTQKFK AAIDGNGFKG
TWTENGSGDV SGKFYGPAGE EVAGKYSYRP TDAEKGGFGV FAGKKEQD*

AG287, with or without His-tag ('AG287-His' and `AG287K', respectively), are expressed at very good levels in comparison with the `287-His' or `287 untagged, On the basis of gene variability data, variants of AG287-His were expressed in E.coli from a number of MenB strains, in particular from strains 2996, MC58, 1000, and BZ232. The results were also good - each of these gave high ELISA titres and also serum bactericidal titres of >8192. AG287K, expressed from pET-24b, gave excellent titres in ELISA and the serum bactericidal assay.

Deletion of poly-Gly sequences is also applicable to Tbp2 (NMB0460), 741 (NMB
1870) and 983 (NMB1969). When cloned in pET vector and expressed in E.coli without the sequence coding for their leader peptides and without poly-Gly (i.e. as "AG
forms"), the same effect was seen - expression was good in the clones carrying the deletion of the poly-glycine stretch, and poor or absent if the glycines were present in the expressed protein.
AG287 was fused directly in-frame upstream of 919, 953, 961 (sequences shown below) and ORF46.1:

ATGGCTAGCCCCGATGTTAAATCGGCGGACACGCTGTCAAAACCGGCCGCTCCTGTTGTTGCTGAAAAAGAGACAGAG
GTAAAAGAAGATGCGCCACAGGCAGGTTCTCAAGGACAGGGCGCGCCATCCACACAAGGCAGCCAAGATATGGCGGCA
GTTTCGGCAGAAAATACAGGCAATGGCGGTGCGGCAACAACGGACAAACCCAAAAATGAAGACGAGGGACCGCAAAAT
GATATGCCGCAAAATTCCGCCGAATCCGCAAATCAAACAGGGAACAACCAACCCGCCGATTCTTCAGATTCCGCCCCC
GCGTCAAACCCTGCACCTGCGAATGGCGGTAGCAATTTTGGAAGGGTTGATTTGGCTAATGGCGTTTTGATTGATGGG
CCGTCGCAAAATATAACGTTGACCCACTGTAAAGGCGATTCTTGTAATGGTGATAATTTATTGGATGAAGAAGCACCG
TCAAAATCAGAATTTGAAAATTTAAATGAGTCTGAACGAATTGAGAAATATAAGAAAGATGGGAAAAGCGATAAATTT
ACTAATTTGGTTGCGACAGCAGTTCAAGCTAATGGAACTAACAAATATGTCATCATTTATAAAGACAAGTCCGCTTCA
TCTTCATCTGCGCGATTCAGGCGTTCTGCACGGTCGAGGAGGTCGCTTCCTGCCGAGATGCCGCTAATCCCCGTCAAT
CAGGCGGATACGCTGATTGTCGATGGGGAAGCGGTCAGCCTGACGGGGCATTCCGGCAATATCTTCGCGCCCGAAGGG
AATTACCGGTATCTGACTTACGGGGCGGAAAAATTGCCCGGCGGATCGTATGCCCTCCGTGTGCAAGGCGAACCGGCA
AAAGGCGAAATGCTTGCTGGCACGGCCGTGTACAACGGCGAAGTGCTGCATTTTCATACGGAAAACGGCCGTCCGTAC
CCGACTAGAGGCAGGTTTGCCGCAAAAGTCGATTTCGGCAGCAAATCTGTGGACGGCATTATCGACAGCGGCGATGAT
TTGCATATGGGTACGCAAAAATTCAAAGCCGCCATCGATGGAAACGGCTTTAAGGGGACTTGGACGGAAAATGGCGGC
GGGGATGTTTCCGGAAGGTTTTACGGCCCGGCCGGCGAGGAAGTGGCGGGAAAATACAGCTATCGCCCGACAGATGCG
GAAAAGGGCGGATTCGGCGTGTTTGCCGGCAAAAAAGAGCAGGATGGATCCGGAGGAGGAGGATGCCAAAGCAAGAGC
ATCCAAACCTTTCCGCAACCCGACACATCCGTCATCAACGGCCCGGACCGGCCGGTCGGCATCCCCGACCCCGCCGGA
ACGACGGTCGGCGGCGGCGGGGCCGTCTATACCGTTGTACCGCACCTGTCCCTGCCCCACTGGGCGGCGCAGGATTTC
GCCAAAAGCCTGCAATCCTTCCGCCTCGGCTGCGCCAATTTGAAAAACCGCCAAGGCTGGCAGGATGTGTGCGCCCAA
GCCTTTCAAACCCCCGTCCATTCCTTTCAGGCAAAACAGTTTTTTGAACGCTATTTCACGCCGTGGCAGGTTGCAGGC
AACGGAAGCCTTGCCGGTACGGTTACCGGCTATTACGAGCCGGTGCTGAAGGGCGACGACAGGCGGACGGCACAAGCC
CGCTTCCCGATTTACGGTATTCCCGACGATTTTATCTCCGTCCCCCTGCCTGCCGGTTTGCGGAGCGGAAAAGCCCTT
GTCCGCATCAGGCAGACGGGAAAAAACAGCGGCACAATCGACAATACCGGCGGCACACATACCGCCGACCTCTCCCGA
TTCCCCATCACCGCGCGCACAACGGCAATCAAAGGCAGGTTTGAAGGAAGCCGCTTCCTCCCCTACCACACGCGCAAC
CAAATCAACGGCGGCGCGCTTGACGGCAAAGCCCCGATACTCGGTTACGCCGAAGACCCCGTCGAACTTTTTTTTATG
CACATCCAAGGCTCGGGCCGTCTGAAAACCCCGTCCGGCAAATACATCCGCATCGGCTATGCCGACAAAAACGAACAT
CCCTACGTTTCCATCGGACGCTATATGGCGGACAAAGGCTACCTCAAGCTCGGGCAGACCTCGATGCAGGGCATCAAA
GCCTATATGCGGCAAAATCCGCAACGCCTCGCCGAAGTTTTGGGTCAAAACCCCAGCTATATCTTTTTCCGCGAGCTT
GCCGGAAGCAGCAATGACGGTCCCGTCGGCGCACTGGGCACGCCGTTGATGGGGGAATATGCCGGCGCAGTCGACCGG
CACTACATTACCTTGGGCGCGCCCTTATTTGTCGCCACCGCCCATCCGGTTACCCGCAAAGCCCTCAACCGCCTGATT
ATGGCGCAGGATACCGGCAGCGCGATTAAAGGCGCGGTGCGCGTGGATTATTTTTGGGGATACGGCGACGAAGCCGGC
GAACTTGCCGGCAAACAGAAAACCACGGGTTACGTCTGGCAGCTCCTACCCAACGGTATGAAGCCCGAATACCGCCCG
TAACTCGAG

801 YGDEAGELAG KQKTTGYVWQ LLPNGMKPEY RP*

ATGGCTAGCCCCGATGTTAAATCGGCGGACACGCTGTCAAAACCGGCCGCTCCTGTTGTTGCTGAAAAAGAGACAGAG
GTAAAAGAAGATGCGCCACAGGCAGGTTCTCAAGGACAGGGCGCGCCATCCACACAAGGCAGCCAAGATATGGCGGCA
GTTTCGGCAGAAAATACAGGCAATGGCGGTGCGGCAACAACGGACAAACCCAAAAATGAAGACGAGGGACCGCAAAAT
GATATGCCGCAAAATTCCGCCGAATCCGCAAATCAAACAGGGAACAACCAACCCGCCGATTCTTCAGATTCCGCCCCC
GCGTCAAACCCTGCACCTGCGAATGGCGGTAGCAATTTTGGAAGGGTTGATTTGGCTAATGGCGTTTTGATTGATGGG
CCGTCGCAAAATATAACGTTGACCCACTGTAAAGGCGATTCTTGTAATGGTGATAATTTATTGGATGAAGAAGCACCG
TCAAAATCAGAATTTGAAAATTTAAATGAGTCTGAACGAATTGAGAAATATAAGAAAGATGGGAAAAGCGATAAATTT
ACTAATTTGGTTGCGACAGCAGTTCAAGCTAATGGAACTAACAAATATGTCATCATTTATAAAGACAAGTCCGCTTCA
TCTTCATCTGCGCGATTCAGGCGTTCTGCACGGTCGAGGAGGTCGCTTCCTGCCGAGATGCCGCTAATCCCCGTCAAT
CAGGCGGATACGCTGATTGTCGATGGGGAAGCGGTCAGCCTGACGGGGCATTCCGGCAATATCTTCGCGCCCGAAGGG
AATTACCGGTATCTGACTTACGGGGCGGAAAAATTGCCCGGCGGATCGTATGCCCTCCGTGTGCAAGGCGAACCGGCA
AAAGGCGAAATGCTTGCTGGCACGGCCGTGTACAACGGCGAAGTGCTGCATTTTCATACGGAAAACGGCCGTCCGTAC
CCGACTAGAGGCAGGTTTGCCGCAAAAGTCGATTTCGGCAGCAAATCTGTGGACGGCATTATCGACAGCGGCGATGAT
TTGCATATGGGTACGCAAAAATTCAAAGCCGCCATCGATGGAAACGGCTTTAAGGGGACTTGGACGGAAAATGGCGGC
GGGGATGTTTCCGGAAGGTTTTACGGCCCGGCCGGCGAGGAAGTGGCGGGAAAATACAGCTATCGCCCGACAGATGCG
GAAAAGGGCGGATTCGGCGTGTTTGCCGGCAAAAAAGAGCAGGATGGATCCGGAGGAGGAGGAGCCACCTACAAAGTG
GACGAATATCACGCCAACGCCCGTTTCGCCATCGACCATTTCAACACCAGCACCAACGTCGGCGGTTTTTACGGTCTG
ACCGGTTCCGTCGAGTTCGACCAAGCAAAACGCGACGGTAAAATCGACATCACCATCCCCGTTGCCAACCTGCAAAGC
GGTTCGCAACACTTTACCGACCACCTGAAATCAGCCGACATCTTCGATGCCGCCCAATATCCGGACATCCGCTTTGTT
TCCACCAAATTCAACTTCAACGGCAAAAAACTGGTTTCCGTTGACGGCAACCTGACCATGCACGGCAAAACCGCCCCC
GTCAAACTCAAAGCCGAAAAATTCAACTGCTACCAAAGCCCGATGGCGAAAACCGAAGTTTGCGGCGGCGACTTCAGC
ACCACCATCGACCGCACCAAATGGGGCGTGGACTACCTCGTTAACGTTGGTATGACCAAAAGCGTCCGCATCGACATC
CAAATCGAGGCAGCCAAACAATAACTCGAG
551 RTKWGVDYLV NVGMTKSVRI DIQIEAAKQ*

ATGGCTAGCCCCGATGTTAAATCGGCGGACACGCTGTCAAAACCGGCCGCTCCTGTTGTTGCTGAAAAAGAGACAGAG
GTAAAAGAAGATGCGCCACAGGCAGGTTCTCAAGGACAGGGCGCGCCATCCACACAAGGCAGCCAAGATATGGCGGCA
GTTTCGGCAGAAAATACAGGCAATGGCGGTGCGGCAACAACGGACAAACCCAAAAATGAAGACGAGGGACCGCAAAAT
GATATGCCGCAAAATTCCGCCGAATCCGCAAATCAAACAGGGAACAACCAACCCGCCGATTCTTCAGATTCCGCCCCC
GCGTCAAACCCTGCACCTGCGAATGGCGGTAGCAATTTTGGAAGGGTTGATTTGGCTAATGGCGTTTTGATTGATGGG
CCGTCGCAAAATATAACGTTGACCCACTGTAAAGGCGATTCTTGTAATGGTGATAATTTATTGGATGAAGAAGCACCG
TCAAAATCAGAATTTGAAAATTTAAATGAGTCTGAACGAATTGAGAAATATAAGAAAGATGGGAAAAGCGATAAATTT
ACTAATTTGGTTGCGACAGCAGTTCAAGCTAATGGAACTAACAAATATGTCATCATTTATAAAGACAAGTCCGCTTCA
TCTTCATCTGCGCGATTCAGGCGTTCTGCACGGTCGAGGAGGTCGCTTCCTGCCGAGATGCCGCTAATCCCCGTCAAT
CAGGCGGATACGCTGATTGTCGATGGGGAAGCGGTCAGCCTGACGGGGCATTCCGGCAATATCTTCGCGCCCGAAGGG
AATTACCGGTATCTGACTTACGGGGCGGAAAAATTGCCCGGCGGATCGTATGCCCTCCGTGTGCAAGGCGAACCGGCA
AAAGGCGAAATGCTTGCTGGCACGGCCGTGTACAACGGCGAAGTGCTGCATTTTCATACGGAAAACGGCCGTCCGTAC
CCGACTAGAGGCAGGTTTGCCGCAAAAGTCGATTTCGGCAGCAAATCTGTGGACGGCATTATCGACAGCGGCGATGAT
TTGCATATGGGTACGCAAAAATTCAAAGCCGCCATCGATGGAAACGGCTTTAAGGGGACTTGGACGGAAAATGGCGGC
GGGGATGTTTCCGGAAGGTTTTACGGCCCGGCCGGCGAGGAAGTGGCGGGAAAATACAGCTATCGCCCGACAGATGCG
GAAAAGGGCGGATTCGGCGTGTTTGCCGGCAAAAAAGAGCAGGATGGATCCGGAGGAGGAGGAGCCACAAACGACGAC
GATGTTAAAAAAGCTGCCACTGTGGCCATTGCTGCTGCCTACAACAATGGCCAAGAAATCAACGGTTTCAAAGCTGGA
GAGACCATCTACGACATTGATGAAGACGGCACAATTACCAAAAAAGACGCAACTGCAGCCGATGTTGAAGCCGACGAC
TTTAAAGGTCTGGGTCTGAAAAAAGTCGTGACTAACCTGACCAAAACCGTCAATGAAAACAAACAAAACGTCGATGCC
AAAGTAAAAGCTGCAGAATCTGAAATAGAAAAGTTAACAACCAAGTTAGCAGACACTGATGCCGCTTTAGCAGATACT
GATGCCGCTCTGGATGCAACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACATTTGCTGAAGAGACTAAG
ACAAATATCGTAAAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCATGCCGAAGCATTCAACGAT
ATCGCCGATTCATTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAATGAAGCCAAACAGACGGCC
GAAGAAACCAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAAGCCGAAGCTGCCGCTGGC
ACAGCTAATACTGCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACATCAAAGCTGATATCGCTACGAAC
AAAGATAATATTGCTAAAAAAGCAAACAGTGCCGACGTGTACACCAGAGAAGAGTCTGACAGCAAATTTGTCAGAATT
GATGGTCTGAACGCTACTACCGAAAAATTGGACACACGCTTGGCTTCTGCTGAAAAATCCATTGCCGATCACGATACT
CGCCTGAACGGTTTGGATAAAACAGTGTCAGACCTGCGCAAAGAAACCCGCCAAGGCCTTGCAGAACAAGCCGCGCTC
TCCGGTCTGTTCCAACCTTACAACGTGGGTCGGTTCAATGTAACGGCTGCAGTCGGCGGCTACAAATCCGAATCGGCA
GTCGCCATCGGTACCGGCTTCCGCTTTACCGAAAACTTTGCCGCCAAAGCAGGCGTGGCAGTCGGCACTTCGTCCGGT
TCTTCCGCAGCCTACCATGTCGGCGTCAATTACGAGTGGTAACTCGAG

751 SESAVAIGTG FRFTENFAAK AGVAVGTSSG SSAAYHVGVN YEW*
ELISA Bactericidal AG287-953-His 3834 65536 AG287-961-His 108627 65536 The bactericidal efficacy (homologous strain) of antibodies raised against the hybrid proteins was compared with antibodies raised against simple mixtures of the component antigens (using 287-GST) for 919 and ORF46.1:

Mixture with 287 Hybrid with AG287 ORF46.1 128 16000 Data for bactericidal activity against heterologous MenB strains and against serotypes A and C were also obtained:

919 ORF46.1 Strain Mixture Hybrid Mixture Hybrid MenA (F6124) 512 32000 - 8192 MenC (C11) >2048 >2048 - -MenC (BZ133) >4096 64000 - 8192 The hybrid proteins with AG287 at the N-terminus are therefore immunologically superior to simple mixtures, with AG287-ORF46.1 being particularly effective, even against heterologous strains. AG28 7-ORF46. 1 K may be expressed in pET-24b.

The same hybrid proteins were made using New Zealand strain 394/98 rather than 2996:

ATGGCTAGCCCCGATGTCAAGTCGGCGGACACGCTGTCAAAACCTGCCGCCCCTGTTGTTTCTGAAAAAGAGACAGAG
GCAAAGGAAGATGCGCCACAGGCAGGTTCTCAAGGACAGGGCGCGCCATCCGCACAAGGCGGTCAAGATATGGCGGCG
GTTTCGGAAGAAAATACAGGCAATGGCGGTGCGGCAGCAACGGACAAACCCAAAAATGAAGACGAGGGGGCGCAAAAT
GATATGCCGCAAAATGCCGCCGATACAGATAGTTTGACACCGAATCACACCCCGGCTTCGAATATGCCGGCCGGAAAT
ATGGAAAACCAAGCACCGGATGCCGGGGAATCGGAGCAGCCGGCAAACCAACCGGATATGGCAAATACGGCGGACGGA
ATGCAGGGTGACGATCCGTCGGCAGGCGGGGAAAATGCCGGCAATACGGCTGCCCAAGGTACAAATCAAGCCGAAAAC
AATCAAACCGCCGGTTCTCAAAATCCTGCCTCTTCAACCAATCCTAGCGCCACGAATAGCGGTGGTGATTTTGGAAGG
ACGAACGTGGGCAATTCTGTTGTGATTGACGGGCCGTCGCAAAATATAACGTTGACCCACTGTAAAGGCGATTCTTGT
AGTGGCAATAATTTCTTGGATGAAGAAGTACAGCTAAAATCAGAATTTGAAAAATTAAGTGATGCAGACAAAATAAGT
AATTACAAGAAAGATGGGAAGAATGACGGGAAGAATGATAAATTTGTCGGTTTGGTTGCCGATAGTGTGCAGATGAAG
GGAATCAATCAATATATTATCTTTTATAAACCTAAACCCACTTCATTTGCGCGATTTAGGCGTTCTGCACGGTCGAGG
CGGTCGCTTCCGGCCGAGATGCCGCTGATTCCCGTCAATCAGGCGGATACGCTGATTGTCGATGGGGAAGCGGTCAGC
CTGACGGGGCATTCCGGCAATATCTTCGCGCCCGAAGGGAATTACCGGTATCTGACTTACGGGGCGGAAAAATTGCCC
GGCGGATCGTATGCCCTCCGTGTTCAAGGCGAACCTTCAAAAGGCGAAATGCTCGCGGGCACGGCAGTGTACAACGGC
GAAGTGCTGCATTTTCATACGGAAAACGGCCGTCCGTCCCCGTCCAGAGGCAGGTTTGCCGCAAAAGTCGATTTCGGC
AGCAAATCTGTGGACGGCATTATCGACAGCGGCGATGGTTTGCATATGGGTACGCAAAAATTCAAAGCCGCCATCGAT
GGAAACGGCTTTAAGGGGACTTGGACGGAAAATGGCGGCGGGGATGTTTCCGGAAAGTTTTACGGCCCGGCCGGCGAG
GAAGTGGCGGGAAAATACAGCTATCGCCCAACAGATGCGGAAAAGGGCGGATTCGGCGTGTTTGCCGGCAAAAAAGAG
CAGGATGGATCCGGAGGAGGAGGATGCCAAAGCAAGAGCATCCAAACCTTTCCGCAACCCGACACATCCGTCATCAAC
GGCCCGGACCGGCCGGTCGGCATCCCCGACCCCGCCGGAACGACGGTCGGCGGCGGCGGGGCCGTCTATACCGTTGTA
CCGCACCTGTCCCTGCCCCACTGGGCGGCGCAGGATTTCGCCAAAAGCCTGCAATCCTTCCGCCTCGGCTGCGCCAAT
TTGAAAAACCGCCAAGGCTGGCAGGATGTGTGCGCCCAAGCCTTTCAAACCCCCGTCCATTCCTTTCAGGCAAAACAG
TTTTTTGAACGCTATTTCACGCCGTGGCAGGTTGCAGGCAACGGAAGCCTTGCCGGTACGGTTACCGGCTATTACGAG
CCGGTGCTGAAGGGCGACGACAGGCGGACGGCACAAGCCCGCTTCCCGATTTACGGTATTCCCGACGATTTTATCTCC
GTCCCCCTGCCTGCCGGTTTGCGGAGCGGAAAAGCCCTTGTCCGCATCAGGCAGACGGGAAAAAACAGCGGCACAATC
GACAATACCGGCGGCACACATACCGCCGACCTCTCCCGATTCCCCATCACCGCGCGCACAACGGCAATCAAAGGCAGG
TTTGAAGGAAGCCGCTTCCTCCCCTACCACACGCGCAACCAAATCAACGGCGGCGCGCTTGACGGCAAAGCCCCGATA
CTCGGTTACGCCGAAGACCCCGTCGAACTTTTTTTTATGCACATCCAAGGCTCGGGCCGTCTGAAAACCCCGTCCGGC
AAATACATCCGCATCGGCTATGCCGACAAAAACGAACATCCCTACGTTTCCATCGGACGCTATATGGCGGACAAAGGC
TACCTCAAGCTCGGGCAGACCTCGATGCAGGGCATCAAAGCCTATATGCGGCAAAATCCGCAACGCCTCGCCGAAGTT
TTGGGTCAAAACCCCAGCTATATCTTTTTCCGCGAGCTTGCCGGAAGCAGCAATGACGGTCCCGTCGGCGCACTGGGC
ACGCCGTTGATGGGGGAATATGCCGGCGCAGTCGACCGGCACTACATTACCTTGGGCGCGCCCTTATTTGTCGCCACC
GCCCATCCGGTTACCCGCAAAGCCCTCAACCGCCTGATTATGGCGCAGGATACCGGCAGCGCGATTAAAGGCGCGGTG
CGCGTGGATTATTTTTGGGGATACGGCGACGAAGCCGGCGAACTTGCCGGCAAACAGAAAACCACGGGTTACGTCTGG
CAGCTCCTACCCAACGGTATGAAGCCCGAATACCGCCCGTAAAAGCTT

851 GSAIKGAVRV DYFWGYGDEA GELAGKQKTT GYVWQLLPNG MKPEYRP*

ATGGCTAGCCCCGATGTCAAGTCGGCGGACACGCTGTCAAAACCTGCCGCCCCTGTTGTTTCTGAAAAAGAGACAGAG
GCAAAGGAAGATGCGCCACAGGCAGGTTCTCAAGGACAGGGCGCGCCATCCGCACAAGGCGGTCAAGATATGGCGGCG
GTTTCGGAAGAAAATACAGGCAATGGCGGTGCGGCAGCAACGGACAAACCCAAAAATGAAGACGAGGGGGCGCAAAAT
GATATGCCGCAAAATGCCGCCGATACAGATAGTTTGACACCGAATCACACCCCGGCTTCGAATATGCCGGCCGGAAAT
ATGGAAAACCAAGCACCGGATGCCGGGGAATCGGAGCAGCCGGCAAACCAACCGGATATGGCAAATACGGCGGACGGA
ATGCAGGGTGACGATCCGTCGGCAGGCGGGGAAAATGCCGGCAATACGGCTGCCCAAGGTACAAATCAAGCCGAAAAC
AATCAAACCGCCGGTTCTCAAAATCCTGCCTCTTCAACCAATCCTAGCGCCACGAATAGCGGTGGTGATTTTGGAAGG
ACGAACGTGGGCAATTCTGTTGTGATTGACGGGCCGTCGCAAAATATAACGTTGACCCACTGTAAAGGCGATTCTTGT
AGTGGCAATAATTTCTTGGATGAAGAAGTACAGCTAAAATCAGAATTTGAAAAATTAAGTGATGCAGACAAAATAAGT
AA
TTACAAGAAAGATGGGAAGAATGACGGGAAGAATGATAAATTTGTCGGTTTGGTTGCCGATAGTGTGCAGATGAAG
GGAATCAATCAATATATTATCTTTTATAAACCTAAACCCACTTCATTTGCGCGATTTAGGCGTTCTGCACGGTCGAGG
CGGTCGCTTCCGGCCGAGATGCCGCTGATTCCCGTCAATCAGGCGGATACGCTGATTGTCGATGGGGAAGCGGTCAGC
CTGACGGGGCATTCCGGCAATATCTTCGCGCCCGAAGGGAATTACCGGTATCTGACTTACGGGGCGGAAAAATTGCCC
GGCGGATCGTATGCCCTCCGTGTTCAAGGCGAACCTTCAAAAGGCGAAATGCTCGCGGGCACGGCAGTGTACAACGGC
GAAGTGCTGCATTTTCATACGGAAAACGGCCGTCCGTCCCCGTCCAGAGGCAGGTTTGCCGCAAAAGTCGATTTCGGC
AGCAAATCTGTGGACGGCATTATCGACAGCGGCGATGGTTTGCATATGGGTACGCAAAAATTCAAAGCCGCCATCGAT
GGAAACGGCTTTAAGGGGACTTGGACGGAAAATGGCGGCGGGGATGTTTCCGGAAAGTTTTACGGCCCGGCCGGCGAG
GAAGTGGCGGGAAAATACAGCTATCGCCCAACAGATGCGGAAAAGGGCGGATTCGGCGTGTTTGCCGGCAAAAAAGAG
CAGGATGGATCCGGAGGAGGAGGAGCCACCTACAAAGTGGACGAATATCACGCCAACGCCCGTTTCGCCATCGACCAT
TTCAACACCAGCACCAACGTCGGCGGTTTTTACGGTCTGACCGGTTCCGTCGAGTTCGACCAAGCAAAACGCGACGGT
AAAATCGACATCACCATCCCCGTTGCCAACCTGCAAAGCGGTTCGCAACACTTTACCGACCACCTGAAATCAGCCGAC
ATCTTCGATGCCGCCCAATATCCGGACATCCGCTTTGTTTCCACCAAATTCAACTTCAACGGCAAAAAACTGGTTTCC
GTTGACGGCAACCTGACCATGCACGGCAAAACCGCCCCCGTCAAACTCAAAGCCGAAAAATTCAACTGCTACCAAAGC
CCGATGGCGAAAACCGAAGTTTGCGGCGGCGACTTCAGCACCACCATCGACCGCACCAAATGGGGCGTGGACTACCTC
GTTAACGTTGGTATGACCAAAAGCGTCCGCATCGACATCCAAATCGAGGCAGCCAAACAATAAAAGCTT
601 AKTEVCGGDF STTIDRTKWG VDYLVNVGMT KSVRIDIQIE AAKQ*

ATGGCTAGCCCCGATGTCAAGTCGGCGGACACGCTGTCAAAACCTGCCGCCCCTGTTGTTTCTGAAAAAGAGACAGAG
GCAAAGGAAGATGCGCCACAGGCAGGTTCTCAAGGACAGGGCGCGCCATCCGCACAAGGCGGTCAAGATATGGCGGCG
GTTTCGGAAGAAAATACAGGCAATGGCGGTGCGGCAGCAACGGACAAACCCAAAAATGAAGACGAGGGGGCGCAAAAT
GATATGCCGCAAAATGCCGCCGATACAGATAGTTTGACACCGAATCACACCCCGGCTTCGAATATGCCGGCCGGAAAT
ATGGAAAACCAAGCACCGGATGCCGGGGAATCGGAGCAGCCGGCAAACCAACCGGATATGGCAAATACGGCGGACGGA
ATGCAGGGTGACGATCCGTCGGCAGGCGGGGAAAATGCCGGCAATACGGCTGCCCAAGGTACAAATCAAGCCGAAAAC
AATCAAACCGCCGGTTCTCAAAATCCTGCCTCTTCAACCAATCCTAGCGCCACGAATAGCGGTGGTGATTTTGGAAGG
ACGAACGTGGGCAATTCTGTTGTGATTGACGGGCCGTCGCAAAATATAACGTTGACCCACTGTAAAGGCGATTCTTGT
AGTGGCAATAATTTCTTGGATGAAGAAGTACAGCTAAAATCAGAATTTGAAAAATTAAGTGATGCAGACAAAATAAGT
AATTACAAGAAAGATGGGAAGAATGACGGGAAGAATGATAAATTTGTCGGTTTGGTTGCCGATAGTGTGCAGATGAAG
GGAATCAATCAATATATTATCTTTTATAAACCTAAACCCACTTCATTTGCGCGATTTAGGCGTTCTGCACGGTCGAGG
CGGTCGCTTCCGGCCGAGATGCCGCTGATTCCCGTCAATCAGGCGGATACGCTGATTGTCGATGGGGAAGCGGTCAGC
CTGACGGGGCATTCCGGCAATATCTTCGCGCCCGAAGGGAATTACCGGTATCTGACTTACGGGGCGGAAAAATTGCCC
GGCGGATCGTATGCCCTCCGTGTTCAAGGCGAACCTTCAAAAGGCGAAATGCTCGCGGGCACGGCAGTGTACAACGGC
GAAGTGCTGCATTTTCATACGGAAAACGGCCGTCCGTCCCCGTCCAGAGGCAGGTTTGCCGCAAAAGTCGATTTCGGC
AGCAAATCTGTGGACGGCATTATCGACAGCGGCGATGGTTTGCATATGGGTACGCAAAAATTCAAAGCCGCCATCGAT
GGAAACGGCTTTAAGGGGACTTGGACGGAAAATGGCGGCGGGGATGTTTCCGGAAAGTTTTACGGCCCGGCCGGCGAG
GAAGTGGCGGGAAAATACAGCTATCGCCCAACAGATGCGGAAAAGGGCGGATTCGGCGTGTTTGCCGGCAAAAAAGAG
CAGGATGGATCCGGAGGAGGAGGAGCCACAAACGACGACGATGTTAAAAAAGCTGCCACTGTGGCCATTGCTGCTGCC
TACAACAATGGCCAAGAAATCAACGGTTTCAAAGCTGGAGAGACCATCTACGACATTGATGAAGACGGCACAATTACC
AAAAAAGACGCAACTGCAGCCGATGTTGAAGCCGACGACTTTAAAGGTCTGGGTCTGAAAAAAGTCGTGACTAACCTG
ACCAAAACCGTCAATGAAAACAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAATCTGAAATAGAAAAGTTAACA
ACCAAGTTAGCAGACACTGATGCCGCTTTAGCAGATACTGATGCCGCTCTGGATGCAACCACCAACGCCTTGAATAAA
TTGGGAGAAAATATAACGACATTTGCTGAAGAGACTAAGACAAATATCGTAAAAATTGATGAAAAATTAGAAGCCGTG
GCTGATACCGTCGACAAGCATGCCGAAGCATTCAACGATATCGCCGATTCATTGGATGAAACCAACACTAAGGCAGAC
GAAGCCGTCAAAACCGCCAATGAAGCCAAACAGACGGCCGAAGAAACCAAACAAAACGTCGATGCCAAAGTAAAAGCT
GCAGAAACTGCAGCAGGCAAAGCCGAAGCTGCCGCTGGCACAGCTAATACTGCAGCCGACAAGGCCGAAGCTGTCGCT
GCAAAAGTTACCGACATCAAAGCTGATATCGCTACGAACAAAGATAATATTGCTAAAAAAGCAAACAGTGCCGACGTG
TACACCAGAGAAGAGTCTGACAGCAAATTTGTCAGAATTGATGGTCTGAACGCTACTACCGAAAAATTGGACACACGC
TTGGCTTCTGCTGAAAAATCCATTGCCGATCACGATACTCGCCTGAACGGTTTGGATAAAACAGTGTCAGACCTGCGC
AAAGAAACCCGCCAAGGCCTTGCAGAACAAGCCGCGCTCTCCGGTCTGTTCCAACCTTACAACGTGGGTCGGTTCAAT
GTAACGGCTGCAGTCGGCGGCTACAAATCCGAATCGGCAGTCGCCATCGGTACCGGCTTCCGCTTTACCGAAAACTTT
GCCGCCAAAGCAGGCGTGGCAGTCGGCACTTCGTCCGGTTCTTCCGCAGCCTACCATGTCGGCGTCAATTACGAGTGG
TAAAAGCTT

851 HVGVNYEW*
Example 3 - hybrids of AG983 Protein 983 has the following sequence:

983 r- LG983 9.51 GGLRYDLLKQ DAFAEKGSAL GWSGNSLTEG TLVGLAGLKL SQPLSDKAVL

1051 FGNGWNGLAR YSYAGSKQYG NHSGRVGVGY RF*

AG983 thus has the following basic sequence:

TSAPD FNAGGTGIGS
NSRATTAKSA AVSYAGIKNE MCKDRSMLCA GRDDVAVTDR DAKINAPPPN
LHTGDFPNPN DAYKNLINLK PAIEAGYTGR GVEVGIVDTG ESVGSISFPE
LYGRKEHGYN ENYKNYTAYM RKEAPEDGGG KDIEASFDDE AVIETEAKPT
DIRHVKEIGH IDLVSHIIGG RSVDGRPAGG IAPDATLHIM NTNDETKNEM
MVAAIRNAWV KLGERGVRIV NNSFGTTSRA GTADLFQIAN SEEQYRQALL
DYSGGDKTDE GIRLMQQSDY GNLSYHIRNK NMLFIFSTGN DAQAQPNTYA
LLPFYEKDAQ KGIITVAGVD RSGEKFKREM YGEPGTEPLE YGSNHCGITA
MWCLSAPYEA SVRFTRTNPI QIAGTSFSAP IVTGTAALLL QKYPWMSNDN
LRTTLLTTAQ DIGAVGVDSK FGWGLLDAGK AMNGPASFPF GDFTADTKGT
SDIAYSFRND ISGTGGLIKK GGSQLQLHGN NTYTGKTIIE GGSLVLYGNN
KSDMRVETKG ALIYNGAASG GSLNSDGIVY LADTDQSGAN ETVHIKGSLQ
LDGKGTLYTR LGKLLKVDGT AIIGGKLYMS ARGKGAGYLN STGRRVPFLS
AAKIGQDYSF FTNIETDGGL LASLDSVEKT AGSEGDTLSY YVRRGNAART
ASAAAHSAPA GLKHAVEQGG SNLENLMVEL DASESSATPE TVETAAADRT
DMPGIRPYGA TFRAAAAVQH ANAADGVRIF NSLAATVYAD STAAHADMQG
RRLKAVSDGL DHNGTGLRVI AQTQQDGGTW EQGGVEGKMR GSTQTVGIAA
KTGENTTAAA TLGMGRSTWS ENSANAKTDS ISLFAGIRHD AGDIGYLKGL
FSYGRYKNSI SRSTGADEHA EGSVNGTLMQ LGALGGVNVP FAATGDLTVE
GGLRYDLLKQ DAFAEKGSAL GWSGNSLTEG TLVGLAGLKL SQPLSDKAVL
FATAGVERDL NGRDYTVTGG FTGATAATGK TGARNMPHTR LVAGLGADVE
FGNGWNGLAR YSYAGSKQYG NHSGRVGVGY RF*

AG983 was expressed as a hybrid, with ORF46.1, 741, 961 or 961c at its C-terminus:
AG983-ORF46.1 ATGACTTCTGCGCCCGACTTCAATGCAGGCGGTACCGGTATCGGCAGCAACAGCAGAGCAACAACAGCGAAATCAGCA
GCAGTATCTTACGCCGGTATCAAGAACGAAATGTGCAAAGACAGAAGCATGCTCTGTGCCGGTCGGGATGACGTTGCG
GTTACAGACAGGGATGCCAAAATCAATGCCCCCCCCCCGAATCTGCATACCGGAGACTTTCCAAACCCAAATGACGCA
TACAAGAATTTGATCAACCTCAAACCTGCAATTGAAGCAGGCTATACAGGACGCGGGGTAGAGGTAGGTATCGTCGAC
ACAGGCGAATCCGTCGGCAGCATATCCTTTCCCGAACTGTATGGCAGAAAAGAACACGGCTATAACGAAAATTACAAA
AACTATACGGCGTATATGCGGAAGGAAGCGCCTGAAGACGGAGGCGGTAAAGACATTGAAGCTTCTTTCGACGATGAG
GCCGTTATAGAGACTGAAGCAAAGCCGACGGATATCCGCCACGTAAAAGAAATCGGACACATCGATTTGGTCTCCCAT
ATTATTGGCGGGCGTTCCGTGGACGGCAGACCTGCAGGCGGTATTGCGCCCGATGCGACGCTACACATAATGAATACG
AATGATGAAACCAAGAACGAAATGATGGTTGCAGCCATCCGCAATGCATGGGTCAAGCTGGGCGAACGTGGCGTGCGC
ATCGTCAATAACAGTTTTGGAACAACATCGAGGGCAGGCACTGCCGACCTTTTCCAAATAGCCAATTCGGAGGAGCAG
TACCGCCAAGCGTTGCTCGACTATTCCGGCGGTGATAAAACAGACGAGGGTATCCGCCTGATGCAACAGAGCGATTAC
GGCAACCTGTCCTACCACATCCGTAATAAAAACATGCTTTTCATCTTTTCGACAGGCAATGACGCACAAGCTCAGCCC
AACACATATGCCCTATTGCCATTTTATGAAAAAGACGCTCAAAAAGGCATTATCACAGTCGCAGGCGTAGACCGCAGT
GGAGAAAAGTTCAAACGGGAAATGTATGGAGAACCGGGTACAGAACCGCTTGAGTATGGCTCCAACCATTGCGGAATT
ACTGCCATGTGGTGCCTGTCGGCACCCTATGAAGCAAGCGTCCGTTTCACCCGTACAAACCCGATTCAAATTGCCGGA
ACATCCTTTTCCGCACCCATCGTAACCGGCACGGCGGCTCTGCTGCTGCAGAAATACCCGTGGATGAGCAACGACAAC
CTGCGTACCACGTTGCTGACGACGGCTCAGGACATCGGTGCAGTCGGCGTGGACAGCAAGTTCGGCTGGGGACTGCTG
GATGCGGGTAAGGCCATGAACGGACCCGCGTCCTTTCCGTTCGGCGACTTTACCGCCGATACGAAAGGTACATCCGAT
ATTGCCTACTCCTTCCGTAACGACATTTCAGGCACGGGCGGCCTGATCAAAAAAGGCGGCAGCCAACTGCAACTGCAC
GGCAACAACACCTATACGGGCAAAACCATTATCGAAGGCGGTTCGCTGGTGTTGTACGGCAACAACAAATCGGATATG
CGCGTCGAAACCAAAGGTGCGCTGATTTATAACGGGGCGGCATCCGGCGGCAGCCTGAACAGCGACGGCATTGTCTAT
CTGGCAGATACCGACCAATCCGGCGCAAACGAAACCGTACACATCAAAGGCAGTCTGCAGCTGGACGGCAAAGGTACG
CTGTACACACGTTTGGGCAAACTGCTGAAAGTGGACGGTACGGCGATTATCGGCGGCAAGCTGTACATGTCGGCACGC
GGCAAGGGGGCAGGCTATCTCAACAGTACCGGACGACGTGTTCCCTTCCTGAGTGCCGCCAAAATCGGGCAGGATTAT
TCTTTCTTCACAAACATCGAAACCGACGGCGGCCTGCTGGCTTCCCTCGACAGCGTCGAAAAAACAGCGGGCAGTGAA
GGCGACACGCTGTCCTATTATGTCCGTCGCGGCAATGCGGCACGGACTGCTTCGGCAGCGGCACATTCCGCGCCCGCC
GGTCTGAAACACGCCGTAGAACAGGGCGGCAGCAATCTGGAAAACCTGATGGTCGAACTGGATGCCTCCGAATCATCC
GCAACACCCGAGACGGTTGAAACTGCGGCAGCCGACCGCACAGATATGCCGGGCATCCGCCCCTACGGCGCAACTTTC
CGCGCAGCGGCAGCCGTACAGCATGCGAATGCCGCCGACGGTGTACGCATCTTCAACAGTCTCGCCGCTACCGTCTAT
GCCGACAGTACCGCCGCCCATGCCGATATGCAGGGACGCCGCCTGAAAGCCGTATCGGACGGGTTGGACCACAACGGC
ACGGGTCTGCGCGTCATCGCGCAAACCCAACAGGACGGTGGAACGTGGGAACAGGGCGGTGTTGAAGGCAAAATGCGC
GGCAGTACCCAAACCGTCGGCATTGCCGCGAAAACCGGCGAAAATACGACAGCAGCCGCCACACTGGGCATGGGACGC
AGCACATGGAGCGAAAACAGTGCAAATGCAAAAACCGACAGCATTAGTCTGTTTGCAGGCATACGGCACGATGCGGGC
GATATCGGCTATCTCAAAGGCCTGTTCTCCTACGGACGCTACAAAAACAGCATCAGCCGCAGCACCGGTGCGGACGAA
CATGCGGAAGGCAGCGTCAACGGCACGCTGATGCAGCTGGGCGCACTGGGCGGTGTCAACGTTCCGTTTGCCGCAACG
GGAGATTTGACGGTCGAAGGCGGTCTGCGCTACGACCTGCTCAAACAGGATGCATTCGCCGAAAAAGGCAGTGCTTTG
GGCTGGAGCGGCAACAGCCTCACTGAAGGCACGCTGGTCGGACTCGCGGGTCTGAAGCTGTCGCAACCCTTGAGCGAT
AAAGCCGTCCTGTTTGCAACGGCGGGCGTGGAACGCGACCTGAACGGACGCGACTACACGGTAACGGGCGGCTTTACC
GGCGCGACTGCAGCAACCGGCAAGACGGGGGCACGCAATATGCCGCACACCCGTCTGGTTGCCGGCCTGGGCGCGGAT
GTCGAATTCGGCAACGGCTGGAACGGCTTGGCACGTTACAGCTACGCCGGTTCCAAACAGTACGGCAACCACAGCGGA
CGAGTCGGCGTAGGCTACCGGTTCCTCGACGGTGGCGGAGGCACTGGATCCTCAGATTTGGCAAACGATTCTTTTATC
CGGCAGGTTCTCGACCGTCAGCATTTCGAACCCGACGGGAAATACCACCTATTCGGCAGCAGGGGGGAACTTGCCGAG
CGCAGCGGCCATATCGGATTGGGAAAAATACAAAGCCATCAGTTGGGCAACCTGATGATTCAACAGGCGGCCATTAAA
GGAAATATCGGCTACATTGTCCGCTTTTCCGATCACGGGCACGAAGTCCATTCCCCCTTCGACAACCATGCCTCACAT
TCCGATTCTGATGAAGCCGGTAGTCCCGTTGACGGATTTAGCCTTTACCGCATCCATTGGGACGGATACGAACACCAT
CCCGCCGACGGCTATGACGGGCCACAGGGCGGCGGCTATCCCGCTCCCAAAGGCGCGAGGGATATATACAGCTACGAC
ATAAAAGGCGTTGCCCAAAATATCCGCCTCAACCTGACCGACAACCGCAGCACCGGACAACGGCTTGCCGACCGTTTC
CACAATGCCGGTAGTATGCTGACGCAAGGAGTAGGCGACGGATTCAAACGCGCCACCCGATACAGCCCCGAGCTGGAC
AGATCGGGCAATGCCGCCGAAGCCTTCAACGGCACTGCAGATATCGTTAAAAACATCATCGGCGCGGCAGGAGAAATT
GTCGGCGCAGGCGATGCCGTGCAGGGCATAAGCGAAGGCTCAAACATTGCTGTCATGCACGGCTTGGGTCTGCTTTCC
ACCGAAAACAAGATGGCGCGCATCAACGATTTGGCAGATATGGCGCAACTCAAAGACTATGCCGCAGCAGCCATCCGC
GATTGGGCAGTCCAAAACCCCAATGCCGCACAAGGCATAGAAGCCGTCAGCAATATCTTTATGGCAGCCATCCCCATC
AAAGGGATTGGAGCTGTTCGGGGAAAATACGGCTTGGGCGGCATCACGGCACATCCTATCAAGCGGTCGCAGATGGGC
GCGATCGCATTGCCGAAAGGGAAATCCGCCGTCAGCGACAATTTTGCCGATGCGGCATACGCCAAATACCCGTCCCCT
TACCATTCCCGAAATATCCGTTCAAACTTGGAGCAGCGTTACGGCAAAGAAAACATCACCTCCTCAACCGTGCCGCCG
TCAAACGGCAAAAATGTCAAACTGGCAGACCAACGCCACCCGAAGACAGGCGTACCGTTTGACGGTAAAGGGTTTCCG
AATTTTGAGAAGCACGTGAAATATGATACGCTCGAGCACCACCACCACCACCACTGA
1451 DGKGFPNFEK HVKYDTLEHH HHHH*

ATGACTTCTGCGCCCGACTTCAATGCAGGCGGTACCGGTATCGGCAGCAACAGCAGAGCAACAACAGCGAAATCAGCA
GCAGTATCTTACGCCGGTATCAAGAACGAAATGTGCAAAGACAGAAGCATGCTCTGTGCCGGTCGGGATGACGTTGCG
GTTACAGACAGGGATGCCAAAATCAATGCCCCCCCCCCGAATCTGCATACCGGAGACTTTCCAAACCCAAATGACGCA
TACAAGAATTTGATCAACCTCAAACCTGCAATTGAAGCAGGCTATACAGGACGCGGGGTAGAGGTAGGTATCGTCGAC
ACAGGCGAATCCGTCGGCAGCATATCCTTTCCCGAACTGTATGGCAGAAAAGAACACGGCTATAACGAAAATTACAAA
AACTATACGGCGTATATGCGGAAGGAAGCGCCTGAAGACGGAGGCGGTAAAGACATTGAAGCTTCTTTCGACGATGAG
GCCGTTATAGAGACTGAAGCAAAGCCGACGGATATCCGCCACGTAAAAGAAATCGGACACATCGATTTGGTCTCCCAT
ATTATTGGCGGGCGTTCCGTGGACGGCAGACCTGCAGGCGGTATTGCGCCCGATGCGACGCTACACATAATGAATACG
AATGATGAAACCAAGAACGAAATGATGGTTGCAGCCATCCGCAATGCATGGGTCAAGCTGGGCGAACGTGGCGTGCGC
ATCGTCAATAACAGTTTTGGAACAACATCGAGGGCAGGCACTGCCGACCTTTTCCAAATAGCCAATTCGGAGGAGCAG
TACCGCCAAGCGTTGCTCGACTATTCCGGCGGTGATAAAACAGACGAGGGTATCCGCCTGATGCAACAGAGCGATTAC
GGCAACCTGTCCTACCACATCCGTAATAAAAACATGCTTTTCATCTTTTCGACAGGCAATGACGCACAAGCTCAGCCC
AACACATATGCCCTATTGCCATTTTATGAAAAAGACGCTCAAAAAGGCATTATCACAGTCGCAGGCGTAGACCGCAGT
GGAGAAAAGTTCAAACGGGAAATGTATGGAGAACCGGGTACAGAACCGCTTGAGTATGGCTCCAACCATTGCGGAATT
ACTGCCATGTGGTGCCTGTCGGCACCCTATGAAGCAAGCGTCCGTTTCACCCGTACAAACCCGATTCAAATTGCCGGA
ACATCCTTTTCCGCACCCATCGTAACCGGCACGGCGGCTCTGCTGCTGCAGAAATACCCGTGGATGAGCAACGACAAC
CTGCGTACCACGTTGCTGACGACGGCTCAGGACATCGGTGCAGTCGGCGTGGACAGCAAGTTCGGCTGGGGACTGCTG
GATGCGGGTAAGGCCATGAACGGACCCGCGTCCTTTCCGTTCGGCGACTTTACCGCCGATACGAAAGGTACATCCGAT
ATTGCCTACTCCTTCCGTAACGACATTTCAGGCACGGGCGGCCTGATCAAAAAAGGCGGCAGCCAACTGCAACTGCAC
GGCAACAACACCTATACGGGCAAAACCATTATCGAAGGCGGTTCGCTGGTGTTGTACGGCAACAACAAATCGGATATG
CGCGTCGAAACCAAAGGTGCGCTGATTTATAACGGGGCGGCATCCGGCGGCAGCCTGAACAGCGACGGCATTGTCTAT
CTGGCAGATACCGACCAATCCGGCGCAAACGAAACCGTACACATCAAAGGCAGTCTGCAGCTGGACGGCAAAGGTACG
CTGTACACACGTTTGGGCAAACTGCTGAAAGTGGACGGTACGGCGATTATCGGCGGCAAGCTGTACATGTCGGCACGC
GGCAAGGGGGCAGGCTATCTCAACAGTACCGGACGACGTGTTCCCTTCCTGAGTGCCGCCAAAATCGGGCAGGATTAT
TCTTTCTTCACAAACATCGAAACCGACGGCGGCCTGCTGGCTTCCCTCGACAGCGTCGAAAAAACAGCGGGCAGTGAA
GGCGACACGCTGTCCTATTATGTCCGTCGCGGCAATGCGGCACGGACTGCTTCGGCAGCGGCACATTCCGCGCCCGCC
GGTCTGAAACACGCCGTAGAACAGGGCGGCAGCAATCTGGAAAACCTGATGGTCGAACTGGATGCCTCCGAATCATCC
GCAACACCCGAGACGGTTGAAACTGCGGCAGCCGACCGCACAGATATGCCGGGCATCCGCCCCTACGGCGCAACTTTC
CGCGCAGCGGCAGCCGTACAGCATGCGAATGCCGCCGACGGTGTACGCATCTTCAACAGTCTCGCCGCTACCGTCTAT
GCCGACAGTACCGCCGCCCATGCCGATATGCAGGGACGCCGCCTGAAAGCCGTATCGGACGGGTTGGACCACAACGGC
ACGGGTCTGCGCGTCATCGCGCAAACCCAACAGGACGGTGGAACGTGGGAACAGGGCGGTGTTGAAGGCAAAATGCGC
GGCAGTACCCAAACCGTCGGCATTGCCGCGAAAACCGGCGAAAATACGACAGCAGCCGCCACACTGGGCATGGGACGC
AGCACATGGAGCGAAAACAGTGCAAATGCAAAAACCGACAGCATTAGTCTGTTTGCAGGCATACGGCACGATGCGGGC
GATATCGGCTATCTCAAAGGCCTGTTCTCCTACGGACGCTACAAAAACAGCATCAGCCGCAGCACCGGTGCGGACGAA
CATGCGGAAGGCAGCGTCAACGGCACGCTGATGCAGCTGGGCGCACTGGGCGGTGTCAACGTTCCGTTTGCCGCAACG
GGAGATTTGACGGTCGAAGGCGGTCTGCGCTACGACCTGCTCAAACAGGATGCATTCGCCGAAAAAGGCAGTGCTTTG
GGCTGGAGCGGCAACAGCCTCACTGAAGGCACGCTGGTCGGACTCGCGGGTCTGAAGCTGTCGCAACCCTTGAGCGAT
AAAGCCGTCCTGTTTGCAACGGCGGGCGTGGAACGCGACCTGAACGGACGCGACTACACGGTAACGGGCGGCTTTACC
GGCGCGACTGCAGCAACCGGCAAGACGGGGGCACGCAATATGCCGCACACCCGTCTGGTTGCCGGCCTGGGCGCGGAT
GTCGAATTCGGCAACGGCTGGAACGGCTTGGCACGTTACAGCTACGCCGGTTCCAAACAGTACGGCAACCACAGCGGA
CGAGTCGGCGTAGGCTACCGGTTCCTCGAGGGATCCGGAGGGGGTGGTGTCGCCGCCGACATCGGTGCGGGGCTTGCC
GATGCACTAACCGCACCGCTCGACCATAAAGACAAAGGTTTGCAGTCTTTGACGCTGGATCAGTCCGTCAGGAAAAAC
GAGAAACTGAAGCTGGCGGCACAAGGTGCGGAAAAAACTTATGGAAACGGTGACAGCCTCAATACGGGCAAATTGAAG
AACGACAAGGTCAGCCGTTTCGACTTTATCCGCCAAATCGAAGTGGACGGGCAGCTCATTACCTTGGAGAGTGGAGAG
TTCCAAGTATACAAACAAAGCCATTCCGCCTTAACCGCCTTTCAGACCGAGCAAATACAAGATTCGGAGCATTCCGGG
AAGATGGTTGCGAAACGCCAGTTCAGAATCGGCGACATAGCGGGCGAACATACATCTTTTGACAAGCTTCCCGAAGGC
GGCAGGGCGACATATCGCGGGACGGCGTTCGGTTCAGACGATGCCGGCGGAAAACTGACCTACACCATAGATTTCGCC
GCCAAGCAGGGAAACGGCAAAATCGAACATTTGAAATCGCCAGAACTCAATGTCGACCTGGCCGCCGCCGATATCAAG
CCGGATGGAAAACGCCATGCCGTCATCAGCGGTTCCGTCCTTTACAACCAAGCCGAGAAAGGCAGTTACTCCCTCGGT
ATCTTTGGCGGAAAAGCCCAGGAAGTTGCCGGCAGCGCGGAAGTGAAAACCGTAAACGGCATACGCCATATCGGCCTT
GCCGCCAAGCAACTCGAGCACCACCACCACCACCACTGA

1301 AAKQLEHHHH HH*

ATGACTTCTGCGCCCGACTTCAATGCAGGCGGTACCGGTATCGGCAGCAACAGCAGAGCAACAACAGCGAAATCAGCA
GCAGTATCTTACGCCGGTATCAAGAACGAAATGTGCAAAGACAGAAGCATGCTCTGTGCCGGTCGGGATGACGTTGCG
GTTACAGACAGGGATGCCAAAATCAATGCCCCCCCCCCGAATCTGCATACCGGAGACTTTCCAAACCCAAATGACGCA
TACAAGAATTTGATCAACCTCAAACCTGCAATTGAAGCAGGCTATACAGGACGCGGGGTAGAGGTAGGTATCGTCGAC
ACAGGCGAATCCGTCGGCAGCATATCCTTTCCCGAACTGTATGGCAGAAAAGAACACGGCTATAACGAAAATTACAAA
AACTATACGGCGTATATGCGGAAGGAAGCGCCTGAAGACGGAGGCGGTAAAGACATTGAAGCTTCTTTCGACGATGAG
GCCGTTATAGAGACTGAAGCAAAGCCGACGGATATCCGCCACGTAAAAGAAATCGGACACATCGATTTGGTCTCCCAT
ATTATTGGCGGGCGTTCCGTGGACGGCAGACCTGCAGGCGGTATTGCGCCCGATGCGACGCTACACATAATGAATACG
AATGATGAAACCAAGAACGAAATGATGGTTGCAGCCATCCGCAATGCATGGGTCAAGCTGGGCGAACGTGGCGTGCGC
ATCGTCAATAACAGTTTTGGAACAACATCGAGGGCAGGCACTGCCGACCTTTTCCAAATAGCCAATTCGGAGGAGCAG
TACCGCCAAGCGTTGCTCGACTATTCCGGCGGTGATAAAACAGACGAGGGTATCCGCCTGATGCAACAGAGCGATTAC
GGCAACCTGTCCTACCACATCCGTAATAAAAACATGCTTTTCATCTTTTCGACAGGCAATGACGCACAAGCTCAGCCC
AACACATATGCCCTATTGCCATTTTATGAAAAAGACGCTCAAAAAGGCATTATCACAGTCGCAGGCGTAGACCGCAGT
GGAGAAAAGTTCAAACGGGAAATGTATGGAGAACCGGGTACAGAACCGCTTGAGTATGGCTCCAACCATTGCGGAATT
ACTGCCATGTGGTGCCTGTCGGCACCCTATGAAGCAAGCGTCCGTTTCACCCGTACAAACCCGATTCAAATTGCCGGA
ACATCCTTTTCCGCACCCATCGTAACCGGCACGGCGGCTCTGCTGCTGCAGAAATACCCGTGGATGAGCAACGACAAC
CTGCGTACCACGTTGCTGACGACGGCTCAGGACATCGGTGCAGTCGGCGTGGACAGCAAGTTCGGCTGGGGACTGCTG
GATGCGGGTAAGGCCATGAACGGACCCGCGTCCTTTCCGTTCGGCGACTTTACCGCCGATACGAAAGGTACATCCGAT
ATTGCCTACTCCTTCCGTAACGACATTTCAGGCACGGGCGGCCTGATCAAAAAAGGCGGCAGCCAACTGCAACTGCAC
GGCAACAACACCTATACGGGCAAAACCATTATCGAAGGCGGTTCGCTGGTGTTGTACGGCAACAACAAATCGGATATG
CGCGTCGAAACCAAAGGTGCGCTGATTTATAACGGGGCGGCATCCGGCGGCAGCCTGAACAGCGACGGCATTGTCTAT
CTGGCAGATACCGACCAATCCGGCGCAAACGAAACCGTACACATCAAAGGCAGTCTGCAGCTGGACGGCAAAGGTACG
CTGTACACACGTTTGGGCAAACTGCTGAAAGTGGACGGTACGGCGATTATCGGCGGCAAGCTGTACATGTCGGCACGC
GGCAAGGGGGCAGGCTATCTCAACAGTACCGGACGACGTGTTCCCTTCCTGAGTGCCGCCAAAATCGGGCAGGATTAT
TCTTTCTTCACAAACATCGAAACCGACGGCGGCCTGCTGGCTTCCCTCGACAGCGTCGAAAAAACAGCGGGCAGTGAA
GGCGACACGCTGTCCTATTATGTCCGTCGCGGCAATGCGGCACGGACTGCTTCGGCAGCGGCACATTCCGCGCCCGCC
GGTCTGAAACACGCCGTAGAACAGGGCGGCAGCAAmcma(:~AAAAC'cmC.AmC_P1mrr CATGCGGAAGGCAGCGTCAACGGCACGCTGATGCAGCTGGGCGCACTGGGCGGTGTCAACGTTCCGTTTGCCGCAACG
GGAGATTTGACGGTCGAAGGCGGTCTGCGCTACGACCTGCTCAAACAGGATGCATTCGCCGAAAAAGGCAGTGCTTTG
GGCTGGAGCGGCAACAGCCTCACTGAAGGCACGCTGGTCGGACTCGCGGGTCTGAAGCTGTCGCAACCCTTGAGCGAT
AAAGCCGTCCTGTTTGCAACGGCGGGCGTGGAACGCGACCTGAACGGACGCGACTACACGGTAACGGGCGGCTTTACC
GGCGCGACTGCAGCAACCGGCAAGACGGGGGCACGCAATATGCCGCACACCCGTCTGGTTGCCGGCCTGGGCGCGGAT
GTCGAATTCGGCAACGGCTGGAACGGCTTGGCACGTTACAGCTACGCCGGTTCCAAACAGTACGGCAACCACAGCGGA
CGAGTCGGCGTAGGCTACCGGTTCCTCGAGGGTGGCGGAGGCACTGGATCCGCCACAAACGACGACGATGTTAAAAAA
GCTGCCACTGTGGCCATTGCTGCTGCCTACAACAATGGCCAAGAAATCAACGGTTTCAAAGCTGGAGAGACCATCTAC
GACATTGATGAAGACGGCACAATTACCAAAAAAGACGCAACTGCAGCCGATGTTGAAGCCGACGACTTTAAAGGTCTG
GGTCTGAAAAAAGTCGTGACTAACCTGACCAAAACCGTCAATGAAAACAAACAAAACGTCGATGCCAAAGTAAAAGCT
GCAGAATCTGAAATAGAAAAGTTAACAACCAAGTTAGCAGACACTGATGCCGCTTTAGCAGATACTGATGCCGCTCTG
GATGCAACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACATTTGCTGAAGAGACTAAGACAAATATCGTA
AAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCATGCCGAAGCATTCAACGATATCGCCGATTCA
TTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAATGAAGCCAAACAGACGGCCGAAGAAACCAAA
CAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAAGCCGAAGCTGCCGCTGGCACAGCTAATACT
GCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACATCAAAGCTGATATCGCTACGAACAAAGATAATATT
GCTAAAAAAGCAAACAGTGCCGACGTGTACACCAGAGAAGAGTCTGACAGCAAATTTGTCAGAATTGATGGTCTGAAC
GCTACTACCGAAAAATTGGACACACGCTTGGCTTCTGCTGAAAAATCCATTGCCGATCACGATACTCGCCTGAACGGT
TTGGATAAAACAGTGTCAGACCTGCGCAAAGAAACCCGCCAAGGCCTTGCAGAACAAGCCGCGCTCTCCGGTCTGTTC
CAACCTTACAACGTGGGTCGGTTCAATGTAACGGCTGCAGTCGGCGGCTACAAATCCGAATCGGCAGTCGCCATCGGT
ACCGGCTTCCGCTTTACCGAAAACTTTGCCGCCAAAGCAGGCGTGGCAGTCGGCACTTCGTCCGGTTCTTCCGCAGCC
TACCATGTCGGCGTCAATTACGAGTGGCTCGAGCACCACCACCACCACCACTGA

1401 VAIGTGFRFT ENFAAKAGVA VGTSSGSSAA YHVGVNYEWL EHHHHHH*
AG983-961c ATGACTTCTGCGCCCGACTTCAATGCAGGCGGTACCGGTATCGGCAGCAACAGCAGAGCAACAACAGCGAAATCAGCA
GCAGTATCTTACGCCGGTATCAAGAACGAAATGTGCAAAGACAGAAGCATGCTCTGTGCCGGTCGGGATGACGTTGCG
GTTACAGACAGGGATGCCAAAATCAATGCCCCCCCCCCGAATCTGCATACCGGAGACTTTCCAAACCCAAATGACGCA
TACAAGAATTTGATCAACCTCAAACCTGCAATTGAAGCAGGCTATACAGGACGCGGGGTAGAGGTAGGTATCGTCGAC
ACAGGCGAATCCGTCGGCAGCATATCCTTTCCCGAACTGTATGGCAGAAAAGAACACGGCTATAACGAAAATTACAAA
AACTATACGGCGTATATGCGGAAGGAAGCGCCTGAAGACGGAGGCGGTAAAGACATTGAAGCTTCTTTCGACGATGAG
GCCGTTATAGAGACTGAAGCAAAGCCGACGGATATCCGCCACGTAAAAGAAATCGGACACATCGATTTGGTCTCCCAT
ATTATTGGCGGGCGTTCCGTGGACGGCAGACCTGCAGGCGGTATTGCGCCCGATGCGACGCTACACATAATGAATACG
AATGATGAAACCAAGAACGAAATGATGGTTGCAGCCATCCGCAATGCATGGGTCAAGCTGGGCGAACGTGGCGTGCGC
ATCGTCAATAACAGTTTTGGAACAACATCGAGGGCAGGCACTGCCGACCTTTTCCAAATAGCCAATTCGGAGGAGCAG
TACCGCCAAGCGTTGCTCGACTATTCCGGCGGTGATAAAACAGACGAGGGTATCCGCCTGATGCAACAGAGCGATTAC
GGCAACCTGTCCTACCACATCCGTAATAAAAACATGCTTTTCATCTTTTCGACAGGCAATGACGCACAAGCTCAGCCC
AACACATATGCCCTATTGCCATTTTATGAAAAAGACGCTCAAAAAGGCATTATCACAGTCGCAGGCGTAGACCGCAGT
GGAGAAAAGTTCAAACGGGAAATGTATGGAGAACCGGGTACAGAACCGCTTGAGTATGGCTCCAACCATTGCGGAATT
ACTGCCATGTGGTGCCTGTCGGCACCCTATGAAGCAAGCGTCCGTTTCACCCGTACAAACCCGATTCAAATTGCCGGA
ACATCCTTTTCCGCACCCATCGTAACCGGCACGGCGGCTCTGCTGCTGCAGAAATACCCGTGGATGAGCAACGACAAC
CTGCGTACCACGTTGCTGACGACGGCTCAGGACATCGGTGCAGTCGGCGTGGACAGCAAGTTCGGCTGGGGACTGCTG
GATGCGGGTAAGGCCATGAACGGACCCGCGTCCTTTCCGTTCGGCGACTTTACCGCCGATACGAAAGGTACATCCGAT
ATTGCCTACTCCTTCCGTAACGACATTTCAGGCACGGGCGGCCTGATCAAAAAAGGCGGCAGCCAACTGCAACTGCAC
GGCAACAACACCTATACGGGCAAAACCATTATCGAAGGCGGTTCGCTGGTGTTGTACGGCAACAACAAATCGGATATG
CGCGTCGAAACCAAAGGTGCGCTGATTTATAACGGGGCGGCATCCGGCGGCAGCCTGAACAGCGACGGCATTGTCTAT
CTGGCAGATACCGACCAATCCGGCGCAAACGAAACCGTACACATCAAAGGCAGTCTGCAGCTGGACGGCAAAGGTACG
CTGTACACACGTTTGGGCAAACTGCTGAAAGTGGACGGTACGGCGATTATCGGCGGCAAGCTGTACATGTCGGCACGC
GGCAAGGGGGCAGGCTATCTCAACAGTACCGGACGACGTGTTCCCTTCCTGAGTGCCGCCAAAATCGGGCAGGATTAT
TCTTTCTTCACAAACATCGAAACCGACGGCGGCCTGCTGGCTTCCCTCGACAGCGTCGAAAAAACAGCGGGCAGTGAA
GGCGACACGCTGTCCTATTATGTCCGTCGCGGCAATGCGGCACGGACTGCTTCGGCAGCGGCACATTCCGCGCCCGCC
GGTCTGAAACACGCCGTAGAACAGGGCGGCAGCAATCTGGAAAACCTGATGGTCGAACTGGATGCCTCCGAATCATCC
GCAACACCCGAGACGGTTGAAACTGCGGCAGCCGACCGCACAGATATGCCGGGCATCCGCCCCTACGGCGCAACTTTC
CGCGCAGCGGCAGCCGTACAGCATGCGAATGCCGCCGACGGTGTACGCATCTTCAACAGTCTCGCCGCTACCGTCTAT
GCCGACAGTACCGCCGCCCATGCCGATATGCAGGGACGCCGCCTGAAAGCCGTATCGGACGGGTTGGACCACAACGGC
ACGGGTCTGCGCGTCATCGCGCAAACCCAACAGGACGGTGGAACGTGGGAACAGGGCGGTGTTGAAGGCAAAATGCGC
GGCAGTACCCAAACCGTCGGCATTGCCGCGAAAACCGGCGAAAATACGACAGCAGCCGCCACACTGGGCATGGGACGC
AGCACATGGAGCGAAAACAGTGCAAATGCAAAAACCGACAGCATTAGTCTGTTTGCAGGCATACGGCACGATGCGGGC
GATATCGGCTATCTCAAAGGCCTGTTCTCCTACGGACGCTACAAAAACAGCATCAGCCGCAGCACCGGTGCGGACGAA
CATGCGGAAGGCAGCGTCAACGGCACGCTGATGCAGCTGGGCGCACTGGGCGGTGTCAACGTTCCGTTTGCCGCAACG
GGAGATTTGACGGTCGAAGGCGGTCTGCGCTACGACCTGCTCAAACAGGATGCATTCGCCGAAAAAGGCAGTGCTTTG
GGCTGGAGCGGCAACAGCCTCACTGAAGGCACGCTGGTCGGACTCGCGGGTCTGAAGCTGTCGCAACCCTTGAGCGAT
AAAGCCGTCCTGTTTGCAACGGCGGGCGTGGAACGCGACCTGAACGGACGCGACTACACGGTAACGGGCGGCTTTACC
GGCGCGACTGCAGCAACCGGCAAGACGGGGGCACGCAATATGCCGCACACCCGTCTGGTTGCCGGCCTGGGCGCGGAT
GTCGAATTCGGCAACGGCTGGAACGGCTTGGCACGTTACAGCTACGCCGGTTCCAAACAGTACGGCAACCACAGCGGA
CGAGTCGGCGTAGGCTACCGGTTCCTCGAGGGTGGCGGAGGCACTGGATCCGCCACAAACGACGACGATGTTAAAAAA
GCTGCCACTGTGGCCATTGCTGCTGCCTACAACAATGGCCAAGAAATCAACGGTTTCAAAGCTGGAGAGACCATCTAC
GACATTGATGAAGACGGCACAATTACCAAAAAAGACGCAACTGCAGCCGATGTTGAAGCCGACGACTTTAAAGGTCTG
GGTCTGAAAAAAGTCGTGACTAACCTGACCAAAACCGTCAATGAAAACAAACAAAACGTCGATGCCAAAGTAAAAGCT
GCAGAATCTGAAATAGAAAAGTTAACAACCAAGTTAGCAGACACTGATGCCGCTTTAGCAGATACTGATGCCGCTCTG
GATGCAACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACATTTGCTGAAGAGACTAAGACAAATATCGTA
AAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCATGCCGAAGCATTCAACGATATCGCCGATTCA
TTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAATGAAGCCAAACAGACGGCCGAAGAAACCAAA
CAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAAGCCGAAGCTGCCGCTGGCACAGCTAATACT
GCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACATCAAAGCTGATATCGCTACGAACAAAGATAATATT
GCTAAAAAAGCAAACAGTGCCGACGTGTACACCAGAGAAGAGTCTGACAGCAAATTTGTCAGAATTGATGGTCTGAAC
GCTACTACCGAAAAATTGGACACACGCTTGGCTTCTGCTGAAAAATCCATTGCCGATCACGATACTCGCCTGAACGGT
TTGGATAAAACAGTGTCAGACCTGCGCAAAGAAACCCGCCAAGGCCTTGCAGAACAAGCCGCGCTCTCCGGTCTGTTC
CAACCTTACAACGTGGGTCTCGAGCACCACCACCACCACCACTGA
1351 NGLDKTVSDL RKETRQGLAE QAALSGLFQP YNVGLEHHHH HH*
Example 4 - hybrids of AG741 Protein 741 has the following sequence:

251 QEVAGSAEVK TVNGIRHIGL AAKQ*
AG741 thus has the following basic sequence:

VAAD IGAGLADALT APLDHKDKGL
QSLTLDQSVR KNEKLKLAAQ GAEKTYGNGD SLNTGKLKND KVSRFDFIRQ
IEVDGQLITL ESGEFQVYKQ SHSALTAFQT EQIQDSEHSG KMVAKRQFRI
GDIAGEHTSF DKLPEGGRAT YRGTAFGSDD AGGKLTYTID FAAKQGNGKI
EHLKSPELNV DLAAADIKPD GKRHAVISGS VLYNQAEKGS YSLGIFGGKA
QEVAGSAEVK TVNGIRHIGL AAKQ*

AG741 was fused directly in-frame upstream of proteins 961, 961c, 983 and ORF46.1:

ATGGTCGCCGCCGACATCGGTGCGGGGCTTGCCGATGCACTAACCGCACCGCTCGACCATAAAGACAAAGGTTTGCAG
TCTTTGACGCTGGATCAGTCCGTCAGGAAAAACGAGAAACTGAAGCTGGCGGCACAAGGTGCGGAAAAAACTTATGGA
AACGGTGACAGCCTCAATACGGGCAAATTGAAGAACGACAAGGTCAGCCGTTTCGACTTTATCCGCCAAATCGAAGTG
GACGGGCAGCTCATTACCTTGGAGAGTGGAGAGTTCCAAGTATACAAACAAAGCCATTCCGCCTTAACCGCCTTTCAG
ACCGAGCAAATACAAGATTCGGAGCATTCCGGGAAGATGGTTGCGAAACGCCAGTTCAGAATCGGCGACATAGCGGGC
GAACATACATCTTTTGACAAGCTTCCCGAAGGCGGCAGGGCGACATATCGCGGGACGGCGTTCGGTTCAGACGATGCC
GGCGGAAAACTGACCTACACCATAGATTTCGCCGCCAAGCAGGGAAACGGCAAAATCGAACATTTGAAATCGCCAGAA
CTCAATGTCGACCTGGCCGCCGCCGATATCAAGCCGGATGGAAAACGCCATGCCGTCATCAGCGGTTCCGTCCTTTAC
AACCAAGCCGAGAAAGGCAGTTACTCCCTCGGTATCTTTGGCGGAAAAGCCCAGGAAGTTGCCGGCAGCGCGGAAGTG
AAAACCGTAAACGGCATACGCCATATCGGCCTTGCCGCCAAGCAACTCGAGGGTGGCGGAGGCACTGGATCCGCCACA
AACGACGACGATGTTAAAAAAGCTGCCACTGTGGCCATTGCTGCTGCCTACAACAATGGCCAAGAAATCAACGGTTTC
AAAGCTGGAGAGACCATCTACGACATTGATGAAGACGGCACAATTACCAAAAAAGACGCAACTGCAGCCGATGTTGAA
GCCGACGACTTTAAAGGTCTGGGTCTGAAAAAAGTCGTGACTAACCTGACCAAAACCGTCAATGAAAACAAACAAAAC
GTCGATGCCAAAGTAAAAGCTGCAGAATCTGAAATAGAAAAGTTAACAACCAAGTTAGCAGACACTGATGCCGCTTTA
GCAGATACTGATGCCGCTCTGGATGCAACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACATTTGCTGAA
GAGACTAAGACAAATATCGTAAAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCATGCCGAAGCA
TTCAACGATATCGCCGATTCATTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAATGAAGCCAAA
CAGACGGCCGAAGAAACCAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAAGCCGAAGCT
GCCGCTGGCACAGCTAATACTGCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACATCAAAGCTGATATC
GCTACGAACAAAGATAATATTGCTAAAAAAGCAAACAGTGCCGACGTGTACACCAGAGAAGAGTCTGACAGCAAATTT
GTCAGAATTGATGGTCTGAACGCTACTACCGAAAAATTGGACACACGCTTGGCTTCTGCTGAAAAATCCATTGCCGAT
CACGATACTCGCCTGAACGGTTTGGATAAAACAGTGTCAGACCTGCGCAAAGAAACCCGCCAAGGCCTTGCAGAACAA
GCCGCGCTCTCCGGTCTGTTCCAACCTTACAACGTGGGTCGGTTCAATGTAACGGCTGCAGTCGGCGGCTACAAATCC
GAATCGGCAGTCGCCATCGGTACCGGCTTCCGCTTTACCGAAAACTTTGCCGCCAAAGCAGGCGTGGCAGTCGGCACT
TCGTCCGGTTCTTCCGCAGCCTACCATGTCGGCGTCAATTACGAGTGGCTCGAGCACCACCACCACCACCACTGA
601 AVAIGTGFRF TENFAAKAGV AVGTSSGSSA AYHVGVNYEW LEHHHHHH*

AG741-961c ATGGTCGCCGCCGACATCGGTGCGGGGCTTGCCGATGCACTAACCGCACCGCTCGACCATAAAGACAAAGGTTTGCAG
TCTTTGACGCTGGATCAGTCCGTCAGGAAAAACGAGAAACTGAAGCTGGCGGCACAAGGTGCGGAAAAAACTTATGGA
AACGGTGACAGCCTCAATACGGGCAAATTGAAGAACGACAAGGTCAGCCGTTTCGACTTTATCCGCCAAATCGAAGTG
GACGGGCAGCTCATTACCTTGGAGAGTGGAGAGTTCCAAGTATACAAACAAAGCCATTCCGCCTTAACCGCCTTTCAG
ACCGAGCAAATACAAGATTCGGAGCATTCCGGGAAGATGGTTGCGAAACGCCAGTTCAGAATCGGCGACATAGCGGGC
GAACATACATCTTTTGACAAGCTTCCCGAAGGCGGCAGGGCGACATATCGCGGGACGGCGTTCGGTTCAGACGATGCC
GGCGGAAAACTGACCTACACCATAGATTTCGCCGCCAAGCAGGGAAACGGCAAAATCGAACATTTGAAATCGCCAGAA
CTCAATGTCGACCTGGCCGCCGCCGATATCAAGCCGGATGGAAAACGCCATGCCGTCATCAGCGGTTCCGTCCTTTAC
AACCAAGCCGAGAAAGGCAGTTACTCCCTCGGTATCTTTGGCGGAAAAGCCCAGGAAGTTGCCGGCAGCGCGGAAGTG
AAAACCGTAAACGGCATACGCCATATCGGCCTTGCCGCCAAGCAACTCGAGGGTGGCGGAGGCACTGGATCCGCCACA
AACGACGACGATGTTAAAAAAGCTGCCACTGTGGCCATTGCTGCTGCCTACAACAATGGCCAAGAAATCAACGGTTTC
AA.AGCTGGAGAGACCATCTACGACATTGATGAAGACGGCACAATTACCAAAAAAGACGCAACTGCAGCCGATGTTGAA

GCCGACGACTTTAAAGGTCTGGGTCTGAAAAAAGTCGTGACTAACCTGACCAAAACCGTCAATGAAAACAAACAAAAC
GTCGATGCCAAAGTAAAAGCTGCAGAATCTGAAATAGAAAAGTTAACAACCAAGTTAGCAGACACTGATGCCGCTTTA
GCAGATACTGATGCCGCTCTGGATGCAACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACATTTGCTGAA
GAGACTAAGACAAATATCGTAAAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCATGCCGAAGCA
TTCAACGATATCGCCGATTCATTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAATGAAGCCAAA
CAGACGGCCGAAGAAACCAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAAGCCGAAGCT
GCCGCTGGCACAGCTAATACTGCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACATCAAAGCTGATATC
GCTACGAACAAAGATAATATTGCTAAAAAAGCAAACAGTGCCGACGTGTACACCAGAGAAGAGTCTGACAGCAAATTT
GTCAGAATTGATGGTCTGAACGCTACTACCGAAAAATTGGACACACGCTTGGCTTCTGCTGAAAAATCCATTGCCGAT
CACGATACTCGCCTGAACGGTTTGGATAAAACAGTGTCAGACCTGCGCAAAGAAACCCGCCAAGGCCTTGCAGAACAA
GCCGCGCTCTCCGGTCTGTTCCAACCTTACAACGTGGGTCTCGAGCACCACCACCACCACCACTGA

551 LNGLDKTVSD LRKETRQGLA EQAALSGLFQ PYNVGLEHHH HHH*

ATGGTCGCCGCCGACATCGGTGCGGGGCTTGCCGATGCACTAACCGCACCGCTCGACCATAAAGACAAAGGTTTGCAG
TCTTTGACGCTGGATCAGTCCGTCAGGAAAAACGAGAAACTGAAGCTGGCGGCACAAGGTGCGGAAAAAACTTATGGA
AACGGTGACAGCCTCAATACGGGCAAATTGAAGAACGACAAGGTCAGCCGTTTCGACTTTATCCGCCAAATCGAAGTG
GACGGGCAGCTCATTACCTTGGAGAGTGGAGAGTTCCAAGTATACAAACAAAGCCATTCCGCCTTAACCGCCTTTCAG
ACCGAGCAAATACAAGATTCGGAGCATTCCGGGAAGATGGTTGCGAAACGCCAGTTCAGAATCGGCGACATAGCGGGC
GAACATACATCTTTTGACAAGCTTCCCGAAGGCGGCAGGGCGACATATCGCGGGACGGCGTTCGGTTCAGACGATGCC
GGCGGAAAACTGACCTACACCATAGATTTCGCCGCCAAGCAGGGAAACGGCAAAATCGAACATTTGAAATCGCCAGAA
CTCAATGTCGACCTGGCCGCCGCCGATATCAAGCCGGATGGAAAACGCCATGCCGTCATCAGCGGTTCCGTCCTTTAC
AACCAAGCCGAGAAAGGCAGTTACTCCCTCGGTATCTTTGGCGGAAAAGCCCAGGAAGTTGCCGGCAGCGCGGAAGTG
A.AAACCGTAAACGGCATACGCCATATCGGCCTTGCCGCCAAGCAACTCGAGGGATCCGGCGGAGGCGGCACTTCTGCG

CCCGACTTCAATGCAGGCGGTACCGGTATCGGCAGCAACAGCAGAGCAACAACAGCGAAATCAGCAGCAGTATCTTAC
GCCGGTATCAAGAACGAAATGTGCAAAGACAGAAGCATGCTCTGTGCCGGTCGGGATGACGTTGCGGTTACAGACAGG
GATGCCAAAATCAATGCCCCCCCCCCGAATCTGCATACCGGAGACTTTCCAAACCCAAATGACGCATACAAGAATTTG
ATCAACCTCAAACCTGCAATTGAAGCAGGCTATACAGGACGCGGGGTAGAGGTAGGTATCGTCGACACAGGCGAATCC
GTCGGCAGCATATCCTTTCCCGAACTGTATGGCAGAAAAGAACACGGCTATAACGAAAATTACAAAAACTATACGGCG
TATATGCGGAAGGAAGCGCCTGAAGACGGAGGCGGTAAAGACATTGAAGCTTCTTTCGACGATGAGGCCGTTATAGAG
ACTGAAGCAAAGCCGACGGATATCCGCCACGTAAAAGAAATCGGACACATCGATTTGGTCTCCCATATTATTGGCGGG
CGTTCCGTGGACGGCAGACCTGCAGGCGGTATTGCGCCCGATGCGACGCTACACATAATGAATACGAATGATGAAACC
AAGAACGAAATGATGGTTGCAGCCATCCGCAATGCATGGGTCAAGCTGGGCGAACGTGGCGTGCGCATCGTCAATAAC
AGTTTTGGAACAACATCGAGGGCAGGCACTGCCGACCTTTTCCAAATAGCCAATTCGGAGGAGCAGTACCGCCAAGCG
TTGCTCGACTATTCCGGCGGTGATAAAACAGACGAGGGTATCCGCCTGATGCAACAGAGCGATTACGGCAACCTGTCC
TACCACATCCGTAATAAAAACATGCTTTTCATCTTTTCGACAGGCAATGACGCACAAGCTCAGCCCAACACATATGCC
CTATTGCCATTTTATGAAAAAGACGCTCAAAAAGGCATTATCACAGTCGCAGGCGTAGACCGCAGTGGAGAAAAGTTC
AAACGGGAAATGTATGGAGAACCGGGTACAGAACCGCTTGAGTATGGCTCCAACCATTGCGGAATTACTGCCATGTGG
TGCCTGTCGGCACCCTATGAAGCAAGCGTCCGTTTCACCCGTACAAACCCGATTCAAATTGCCGGAACATCCTTTTCC
GCACCCATCGTAACCGGCACGGCGGCTCTGCTGCTGCAGAAATACCCGTGGATGAGCAACGACAACCTGCGTACCACG
TTGCTGACGACGGCTCAGGACATCGGTGCAGTCGGCGTGGACAGCAAGTTCGGCTGGGGACTGCTGGATGCGGGTAAG
GCCATGAACGGACCCGCGTCCTTTCCGTTCGGCGACTTTACCGCCGATACGAAAGGTACATCCGATATTGCCTACTCC
TTCCGTAACGACATTTCAGGCACGGGCGGCCTGATCAAAAAAGGCGGCAGCCAACTGCAACTGCACGGCAACAACACC
TATACGGGCAAAACCATTATCGAAGGCGGTTCGCTGGTGTTGTACGGCAACAACAAATCGGATATGCGCGTCGAAACC
AAAGGTGCGCTGATTTATAACGGGGCGGCATCCGGCGGCAGCCTGAACAGCGACGGCATTGTCTATCTGGCAGATACC
GACCAATCCGGCGCAAACGAAACCGTACACATCAAAGGCAGTCTGCAGCTGGACGGCAAAGGTACGCTGTACACACGT
TTGGGCAAACTGCTGAAAGTGGACGGTACGGCGATTATCGGCGGCAAGCTGTACATGTCGGCACGCGGCAAGGGGGCA
GGCTATCTCAACAGTACCGGACGACGTGTTCCCTTCCTGAGTGCCGCCAAAATCGGGCAGGATTATTCTTTCTTCACA
AACATCGAAACCGACGGCGGCCTGCTGGCTTCCCTCGACAGCGTCGAAAAAACAGCGGGCAGTGAAGGCGACACGCTG
TCCTATTATGTCCGTCGCGGCAATGCGGCACGGACTGCTTCGGCAGCGGCACATTCCGCGCCCGCCGGTCTGAAACAC
GCCGTAGAACAGGGCGGCAGCAATCTGGAAAACCTGATGGTCGAACTGGATGCCTCCGAATCATCCGCAACACCCGAG
ACGGTTGAAACTGCGGCAGCCGACCGCACAGATATGCCGGGCATCCGCCCCTACGGCGCAACTTTCCGCGCAGCGGCA
GCCGTACAGCATGCGAATGCCGCCGACGGTGTACGCATCTTCAACAGTCTCGCCGCTACCGTCTATGCCGACAGTACC
GCCGCCCATGCCGATATGCAGGGACGCCGCCTGAAAGCCGTATCGGACGGGTTGGACCACAACGGCACGGGTCTGCGC
GTCATCGCGCAAACCCAACAGGACGGTGGAACGTGGGAACAGGGCGGTGTTGAAGGCAAAATGCGCGGCAGTACCCAA
ACCGTCGGCATTGCCGCGAAAACCGGCGAAAATACGACAGCAGCCGCCACACTGGGCATGGGACGCAGCACATGGAGC
GAAAACAGTGCAAATGCAAAAACCGACAGCATTAGTCTGTTTGCAGGCATACGGCACGATGCGGGCGATATCGGCTAT
CTCAAAGGCCTGTTCTCCTACGGACGCTACAAAAACAGCATCAGCCGCAGCACCGGTGCGGACGAACATGCGGAAGGC
AGCGTCAACGGCACGCTGATGCAGCTGGGCGCACTGGGCGGTGTCAACGTTCCGTTTGCCGCAACGGGAGATTTGACG
GTCGAAGGCGGTCTGCGCTACGACCTGCTCAAACAGGATGCATTCGCCGAAAAAGGCAGTGCTTTGGGCTGGAGCGGC
AACAGCCTCACTGAAGGCACGCTGGTCGGACTCGCGGGTCTGAAGCTGTCGCAACCCTTGAGCGATAAAGCCGTCCTG
TTTGCAACGGCGGGCGTGGAACGCGACCTGAACGGACGCGACTACACGGTAACGGGCGGCTTTACCGGCGCGACTGCA
GCAACCGGCAAGACGGGGGCACGCAATATGCCGCACACCCGTCTGGTTGCCGGCCTGGGCGCGGATGTCGAATTCGGC
AACGGCTGGAACGGCTTGGCACGTTACAGCTACGCCGGTTCCAAACAGTACGGCAACCACAGCGGACGAGTCGGCGTA
GGCTACCGGTTCCTCGAGCACCACCACCACCACCACTGA

1301 GYRFLEHHHH HH*
AG741-ORF46.1 ATGGTCGCCGCCGACATCGGTGCGGGGCTTGCCGATGCACTAACCGCACCGCTCGACCATAAAGACAAAGGTTTGCAG
TCTTTGACGCTGGATCAGTCCGTCAGGAAAAACGAGAAACTGAAGCTGGCGGCACAAGGTGCGGAAAAAACTTATGGA
AACGGTGACAGCCTCAATACGGGCAAATTGAAGAACGACAAGGTCAGCCGTTTCGACTTTATCCGCCAAATCGAAGTG
GACGGGCAGCTCATTACCTTGGAGAGTGGAGAGTTCCAAGTATACAAACAAAGCCATTCCGCCTTAACCGCCTTTCAG
ACCGAGCAAATACAAGATTCGGAGCATTCCGGGAAGATGGTTGCGAAACGCCAGTTCAGAATCGGCGACATAGCGGGC
GAACATACATCTTTTGACAAGCTTCCCGAAGGCGGCAGGGCGACATATCGCGGGACGGCGTTCGGTTCAGACGATGCC
GGCGGAAAACTGACCTACACCATAGATTTCGCCGCCAAGCAGGGAAACGGCAAAATCGAACATTTGAAATCGCCAGAA
CTCAATGTCGACCTGGCCGCCGCCGATATCAAGCCGGATGGAAAACGCCATGCCGTCATCAGCGGTTCCGTCCTTTAC
AA
CCAAGCCGAGAAAGGCAGTTACTCCCTCGGTATCTTTGGCGGAAAAGCCCAGGAAGTTGCCGGCAGCGCGGAAGTG
AAAACCGTAAACGGCATACGCCATATCGGCCTTGCCGCCAAGCAACTCGACGGTGGCGGAGGCACTGGATCCTCAGAT
TTGGCAAACGATTCTTTTATCCGGCAGGTTCTCGACCGTCAGCATTTCGAACCCGACGGGAAATACCACCTATTCGGC
AGCAGGGGGGAACTTGCCGAGCGCAGCGGCCATATCGGATTGGGAAAAATACAAAGCCATCAGTTGGGCAACCTGATG
ATTCAACAGGCGGCCATTAAAGGAAATATCGGCTACATTGTCCGCTTTTCCGATCACGGGCACGAAGTCCATTCCCCC
TTCGACAACCATGCCTCACATTCCGATTCTGATGAAGCCGGTAGTCCCGTTGACGGATTTAGCCTTTACCGCATCCAT
TGGGACGGATACGAACACCATCCCGCCGACGGCTATGACGGGCCACAGGGCGGCGGCTATCCCGCTCCCAAAGGCGCG
AGGGATATATACAGCTACGACATAAAAGGCGTTGCCCAAAATATCCGCCTCAACCTGACCGACAACCGCAGCACCGGA
CAACGGCTTGCCGACCGTTTCCACAATGCCGGTAGTATGCTGACGCAAGGAGTAGGCGACGGATTCAAACGCGCCACC
CGATACAGCCCCGAGCTGGACAGATCGGGCAATGCCGCCGAAGCCTTCAACGGCACTGCAGATATCGTTAAAAACATC
ATCGGCGCGGCAGGAGAAATTGTCGGCGCAGGCGATGCCGTGCAGGGCATAAGCGAAGGCTCAAACATTGCTGTCATG
CACGGCTTGGGTCTGCTTTCCACCGAAAACAAGATGGCGCGCATCAACGATTTGGCAGATATGGCGCAACTCAAAGAC
TATGCCGCAGCAGCCATCCGCGATTGGGCAGTCCAAAACCCCAATGCCGCACAAGGCATAGAAGCCGTCAGCAATATC
TTTATGGCAGCCATCCCCATCAAAGGGATTGGAGCTGTTCGGGGAAAATACGGCTTGGGCGGCATCACGGCACATCCT
ATCAAGCGGTCGCAGATGGGCGCGATCGCATTGCCGAAAGGGAAATCCGCCGTCAGCGACAATTTTGCCGATGCGGCA
TACGCCAAATACCCGTCCCCTTACCATTCCCGAAATATCCGTTCAAACTTGGAGCAGCGTTACGGCAAAGAAAACATC
ACCTCCTCAACCGTGCCGCCGTCAAACGGCAAAAATGTCAAACTGGCAGACCAACGCCACCCGAAGACAGGCGTACCG
TTTGACGGTAAAGGGTTTCCGAATTTTGAGAAGCACGTGAAATATGATACGCTCGAGCACCACCACCACCACCACTGA

651 FDGKGFPNFE KHVKYDTLEH HHHHH*

Example 5 - hybrids of 287 Expression of 287 as full-length with a C-terminal His-tag, or without its leader peptide but with a C-terminal His-tag, gives fairly low expression levels. Better expression is achieved using a N-terminal GST-fusion. As an alternative to using GST as an N-terminal fusion partner, 287 was placed at the C-terminus of protein 919 ('919-287'), of protein 953 ('953-287'), and of proteins ORF46.1 (`ORF46.1-287'). In both cases, the leader peptides were deleted, and the hybrids were direct in-frame fusions.

To generate the 953-287 hybrid, the leader peptides of the two proteins were omitted by designing the forward primer downstream from the leader of each sequence; the stop codon sequence was omitted in the 953 reverse primer but included in the 287 reverse primer. For the 953 gene, the 5' and the 3' primers used for amplification included a NdeI
and a BamHI
restriction sites respectively, whereas for the amplification of the 287 gene the 5' and the 3' primers included a BamHI and a XhoI restriction sites respectively. In this way a sequential directional cloning of the two genes in pET21b+, using Ndel-BamHI (to clone the first gene) and subsequently BamHI-XhoI (to clone the second gene) could be achieved.
The 919-287 hybrid was obtained by cloning the sequence coding for the mature portion of 287 into the Xhol site at the 3'-end of the 919-His clone in pET21b+. The primers used for amplification of the 287 gene were designed for introducing a Sall restriction site at the 5'-and a XhoI site at the 3'- of the PCR fragment. Since the cohesive ends produced by the Sall and XhoI restriction enzymes are compatible, the 287 PCR product digested with Sall-Xhol could be inserted in the pET2lb-919 clone cleaved with XhoI.

The ORF46.1-287 hybrid was obtained similarly.

The bactericidal efficacy (homologous strain) of antibodies raised against the hybrid proteins was compared with antibodies raised against simple mixtures of the component antigens:
Mixture with 287 Hybrid with 287 ORF46.1 128 8192 Data for bactericidal activity against heterologous MenB strains and against serotypes A and C were also obtained for 919-287 and 953-287:

919 953 ORF46.1 Strain Mixture Hybrid Mixture Hybrid Mixture Hybrid MenA (F6124) 512 2048 2048 32 - 1024 MenC (C11) >2048 n.d. >2048 n.d. - n.d.
MenC (BZ133) >4096 >8192 >4096 <16 - 2048 Hybrids of ORF46.1 and 919 were also constructed. Best results (four-fold higher titre) were achieved with 919 at the N-terminus.

Hybrids 919-519His, ORF97-225His and 225-ORF97His were also tested. These gave moderate ELISA fitres and bactericidal antibody responses.

As hybrids of two proteins A & B may be either NH2-A-B-COOH or NH2-B-A-COOH, the "reverse" hybrids with 287 at the N-terminus were also made, but using AG287. A panel of strains was used, including homologous strain 2996. FCA was used as adjuvant:
287 & 919 287 & 953 287 & ORF46.1 Strain 4G287--919 919-287 4G287--953 953-287 4G287--461 46.1-287 BZ232 256 128 128 <4 <4 <4 1000 2048 <4 <4 <4 <4 <4 NGH38 32000 2048 >2048 4096 16384 4096 MenA (F6124) 32000 2048 >2048 32 8192 1024 MenC (BZ133) 64000 >8192 >8192 <16 8192 2048 Better bactericidal titres are generally seen with 287 at the N-terminus.

When fused to protein 961 [NH2-AG287-961-COOH - sequence shown above], the resulting protein is insoluble and must be denatured and renatured for purification.
Following renaturation, around 50% of the protein was found to remain insoluble. The soluble and insoluble proteins were compared, and much better bactericidal titres were obtained with the soluble protein (FCA as adjuvant):

Soluble 65536 128 4096 >2048 >2048 4096 Insoluble 8192 <4 <4 16 n.d. n.d.
Titres with the insoluble form were, however, improved by using alum adjuvant instead:
Insoluble 32768 128 4096 >2048 >2048 2048 961c was also used in hybrid proteins (see above). As 961 and its domain variants direct efficient expression, they are ideally suited as the N-terminal portion of a hybrid protein.
Example 23 -further hybrids Further hybrid proteins of the invention are shown in the drawings and have the sequences set out below. These are advantageous when compared to the individual proteins:

ORF46.1-741 ATGTCAGATTTGGCAAACGATTCTTTTATCCGGCAGGTTCTCGACCGTCAGCATTTCGAACCCGACGGGAAATACCAC
CTATTCGGCAGCAGGGGGGAACTTGCCGAGCGCAGCGGCCATATCGGATTGGGAAAAATACAAAGCCATCAGTTGGGC
AACCTGATGATTCAACAGGCGGCCATTAAAGGAAATATCGGCTACATTGTCCGCTTTTCCGATCACGGGCACGAAGTC
CATTCCCCCTTCGACAACCATGCCTCACATTCCGATTCTGATGAAGCCGGTAGTCCCGTTGACGGATTTAGCCTTTAC
CGCATCCATTGGGACGGATACGAACACCATCCCGCCGACGGCTATGACGGGCCACAGGGCGGCGGCTATCCCGCTCCC
AAAGGCGCGAGGGATATATACAGCTACGACATAAAAGGCGTTGCCCAAAATATCCGCCTCAACCTGACCGACAACCGC
AGCACCGGACAACGGCTTGCCGACCGTTTCCACAATGCCGGTAGTATGCTGACGCAAGGAGTAGGCGACGGATTCAAA
CGCGCCACCCGATACAGCCCCGAGCTGGACAGATCGGGCAATGCCGCCGAAGCCTTCAACGGCACTGCAGATATCGTT
AAAAACATCATCGGCGCGGCAGGAGAAATTGTCGGCGCAGGCGATGCCGTGCAGGGCATAAGCGAAGGCTCAAACATT
GCTGTCATGCACGGCTTGGGTCTGCTTTCCACCGAAAACAAGATGGCGCGCATCAACGATTTGGCAGATATGGCGCAA
CTCAAAGACTATGCCGCAGCAGCCATCCGCGATTGGGCAGTCCAAAACCCCAATGCCGCACAAGGCATAGAAGCCGTC
AGCAATATCTTTATGGCAGCCATCCCCATCAAAGGGATTGGAGCTGTTCGGGGAAAATACGGCTTGGGCGGCATCACG
GCACATCCTATCAAGCGGTCGCAGATGGGCGCGATCGCATTGCCGAAAGGGAAATCCGCCGTCAGCGACAATTTTGCC
GATGCGGCATACGCCAAATACCCGTCCCCTTACCATTCCCGAAATATCCGTTCAAACTTGGAGCAGCGTTACGGCAAA
GAAAACATCACCTCCTCAACCGTGCCGCCGTCAAACGGCAAAAATGTCAAACTGGCAGACCAACGCCACCCGAAGACA
GGCGTACCGTTTGACGGTAAAGGGTTTCCGAATTTTGAGAAGCACGTGAAATATGATACGGGATCCGGAGGGGGTGGT
GTCGCCGCCGACATCGGTGCGGGGCTTGCCGATGCACTAACCGCACCGCTCGACCATAAAGACAAAGGTTTGCAGTCT
TTGACGCTGGATCAGTCCGTCAGGAAAAACGAGAAACTGAAGCTGGCGGCACAAGGTGCGGAAAAAACTTATGGAAAC
GGTGACAGCCTCAATACGGGCAAATTGAAGAACGACAAGGTCAGCCGTTTCGACTTTATCCGCCAAATCGAAGTGGAC
GGGCAGCTCATTACCTTGGAGAGTGGAGAGTTCCAAGTATACAAACAAAGCCATTCCGCCTTAACCGCCTTTCAGACC
GAGCAAATACAAGATTCGGAGCATTCCGGGAAGATGGTTGCGAAACGCCAGTTCAGAATCGGCGACATAGCGGGCGAA
CATACATCTTTTGACAAGCTTCCCGAAGGCGGCAGGGCGACATATCGCGGGACGGCGTTCGGTTCAGACGATGCCGGC
GGAAAACTGACCTACACCATAGATTTCGCCGCCAAGCAGGGAAACGGCAAAATCGAACATTTGAAATCGCCAGAACTC
AATGTCGACCTGGCCGCCGCCGATATCAAGCCGGATGGAAAACGCCATGCCGTCATCAGCGGTTCCGTCCTTTACAAC
CAAGCCGAGAAAGGCAGTTACTCCCTCGGTATCTTTGGCGGAAAAGCCCAGGAAGTTGCCGGCAGCGCGGAAGTGAAA
ACCGTAAACGGCATACGCCATATCGGCCTTGCCGCCAAGCAACTCGAGCACCACCACCACCACCACTGA

651 TVNGIRHIGL AAKQLEHHHH HH*

ORF46.1-961 ATGTCAGATTTGGCAAACGATTCTTTTATCCGGCAGGTTCTCGACCGTCAGCATTTCGAACCCGACGGGAAATACCAC
CTATTCGGCAGCAGGGGGGAACTTGCCGAGCGCAGCGGCCATATCGGATTGGGAAAAATACAAAGCCATCAGTTGGGC
AACCTGATGATTCAACAGGCGGCCATTAAAGGAAATATCGGCTACATTGTCCGCTTTTCCGATCACGGGCACGAAGTC
CATTCCCCCTTCGACAACCATGCCTCACATTCCGATTCTGATGAAGCCGGTAGTCCCGTTGACGGATTTAGCCTTTAC
CGCATCCATTGGGACGGATACGAACACCATCCCGCCGACGGCTATGACGGGCCACAGGGCGGCGGCTATCCCGCTCCC
AAAGGCGCGAGGGATATATACAGCTACGACATAAAAGGCGTTGCCCAAAATATCCGCCTCAACCTGACCGACAACCGC
AGCACCGGACAACGGCTTGCCGACCGTTTCCACAATGCCGGTAGTATGCTGACGCAAGGAGTAGGCGACGGATTCAAA
CGCGCCACCCGATACAGCCCCGAGCTGGACAGATCGGGCAATGCCGCCGAAGCCTTCAACGGCACTGCAGATATCGTT
AAAAACATCATCGGCGCGGCAGGAGAAATTGTCGGCGCAGGCGATGCCGTGCAGGGCATAAGCGAAGGCTCAAACATT
GCTGTCATGCACGGCTTGGGTCTGCTTTCCACCGAAAACAAGATGGCGCGCATCAACGATTTGGCAGATATGGCGCAA
CTCAAAGACTATGCCGCAGCAGCCATCCGCGATTGGGCAGTCCAAAACCCCAATGCCGCACAAGGCATAGAAGCCGTC
AGCAATATCTTTATGGCAGCCATCCCCATCAAAGGGATTGGAGCTGTTCGGGGAAAATACGGCTTGGGCGGCATCACG
GCACATCCTATCAAGCGGTCGCAGATGGGCGCGATCGCATTGCCGAAAGGGAAATCCGCCGTCAGCGACAATTTTGCC
GATGCGGCATACGCCAAATACCCGTCCCCTTACCATTCCCGAAATATCCGTTCAAACTTGGAGCAGCGTTACGGCAAA
GAAAACATCACCTCCTCAACCGTGCCGCCGTCAAACGGCAAAAATGTCAAACTGGCAGACCAACGCCACCCGAAGACA
GGCGTACCGTTTGACGGTAAAGGGTTTCCGAATTTTGAGAAGCACGTGAAATATGATACGGGATCCGGAGGAGGAGGA
GCCACAAACGACGACGATGTTAAAAAAGCTGCCACTGTGGCCATTGCTGCTGCCTACAACAATGGCCAAGAAATCAAC
GGTTTCAAAGCTGGAGAGACCATCTACGACATTGATGAAGACGGCACAATTACCAAAAAAGACGCAACTGCAGCCGAT
GTTGAAGCCGACGACTTTAAAGGTCTGGGTCTGAAAAAAGTCGTGACTAACCTGACCAAAACCGTCAATGAAAACAAA
CAAAACGTCGATGCCAAAGTAAAAGCTGCAGAATCTGAAATAGAAAAGTTAACAACCAAGTTAGCAGACACTGATGCC
GCTTTAGCAGATACTGATGCCGCTCTGGATGCAACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACATTT
GCTGAAGAGACTAAGACAAATATCGTAAAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCATGCC
GAAGCATTCAACGATATCGCCGATTCATTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAATGAA
GCCAAACAGACGGCCGAAGAAACCAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAAGCC
GAAGCTGCCGCTGGCACAGCTAATACTGCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACATCAAAGCT
GATATCGCTACGAACAAAGATAATATTGCTAAAAAAGCAAACAGTGCCGACGTGTACACCAGAGAAGAGTCTGACAGC
AAATTTGTCAGAATTGATGGTCTGAACGCTACTACCGAAAAATTGGACACACGCTTGGCTTCTGCTGAAAAATCCATT
GCCGATCACGATACTCGCCTGAACGGTTTGGATAAAACAGTGTCAGACCTGCGCAAAGAAACCCGCCAAGGCCTTGCA
GAACAAGCCGCGCTCTCCGGTCTGTTCCAACCTTACAACGTGGGTCGGTTCAATGTAACGGCTGCAGTCGGCGGCTAC
AAATCCGAATCGGCAGTCGCCATCGGTACCGGCTTCCGCTTTACCGAAAACTTTGCCGCCAAAGCAGGCGTGGCAGTC
GGCACTTCGTCCGGTTCTTCCGCAGCCTACCATGTCGGCGTCAATTACGAGTGGCTCGAGCACCACCACCACCACCAC
TGA
801 HHHHHH*
ORF46.1-961c ATGTCAGATTTGGCAAACGATTCTTTTATCCGGCAGGTTCTCGACCGTCAGCATTTCGAACCCGACGGGAAATACCAC
CTATTCGGCAGCAGGGGGGAACTTGCCGAGCGCAGCGGCCATATCGGATTGGGAAAAATACAAAGCCATCAGTTGGGC
AACCTGATGATTCAACAGGCGGCCATTAAAGGAAATATCGGCTACATTGTCCGCTTTTCCGATCACGGGCACGAAGTC
CATTCCCCCTTCGACAACCATGCCTCACATTCCGATTCTGATGAAGCCGGTAGTCCCGTTGACGGATTTAGCCTTTAC
CGCATCCATTGGGACGGATACGAACACCATCCCGCCGACGGCTATGACGGGCCACAGGGCGGCGGCTATCCCGCTCCC
AAAGGCGCGAGGGATATATACAGCTACGACATAAAAGGCGTTGCCCAAAATATCCGCCTCAACCTGACCGACAACCGC
AGCACCGGACAACGGCTTGCCGACCGTTTCCACAATGCCGGTAGTATGCTGACGCAAGGAGTAGGCGACGGATTCAAA
CGCGCCACCCGATACAGCCCCGAGCTGGACAGATCGGGCAATGCCGCCGAAGCCTTCAACGGCACTGCAGATATCGTT
AAAAACATCATCGGCGCGGCAGGAGAAATTGTCGGCGCAGGCGATGCCGTGCAGGGCATAAGCGAAGGCTCAAACATT
GCTGTCATGCACGGCTTGGGTCTGCTTTCCACCGAAAACAAGATGGCGCGCATCAACGATTTGGCAGATATGGCGCAA
CTCAAAGACTATGCCGCAGCAGCCATCCGCGATTGGGCAGTCCAAAACCCCAATGCCGCACAAGGCATAGAAGCCGTC
AGCAATATCTTTATGGCAGCCATCCCCATCAAAGGGATTGGAGCTGTTCGGGGAAAATACGGCTTGGGCGGCATCACG
GCACATCCTATCAAGCGGTCGCAGATGGGCGCGATCGCATTGCCGAAAGGGAAATCCGCCGTCAGCGACAATTTTGCC
GATGCGGCATACGCCAAATACCCGTCCCCTTACCATTCCCGAAATATCCGTTCAAACTTGGAGCAGCGTTACGGCAAA
GAAAACATCACCTCCTCAACCGTGCCGCCGTCAAACGGCAAAAATGTCAAACTGGCAGACCAACGCCACCCGAAGACA
GGCGTACCGTTTGACGGTAAAGGGTTTCCGAATTTTGAGAAGCACGTGAAATATGATACGGGATCCGGAGGAGGAGGA
GCCACAAACGACGACGATGTTAAAAAAGCTGCCACTGTGGCCATTGCTGCTGCCTACAACAATGGCCAAGAAATCAAC
GGTTTCAAAGCTGGAGAGACCATCTACGACATTGATGAAGACGGCACAATTACCAAAAAAGACGCAACTGCAGCCGAT
GTTGAAGCCGACGACTTTAAAGGTCTGGGTCTGAAAAAAGTCGTGACTAACCTGACCAAAACCGTCAATGAAAACAAA
CAAAACGTCGATGCCAAAGTAAAAGCTGCAGAATCTGAAATAGAAAAGTTAACAACCAAGTTAGCAGACACTGATGCC
GCTTTAGCAGATACTGATGCCGCTCTGGATGCAACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACATTT
GCTGAAGAGACTAAGACAAATATCGTAAAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCATGCC
GAAGCATTCAACGATATCGCCGATTCATTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAATGAA
GCCAAACAGACGGCCGAAGAAACCAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAAGCC
GAAGCTGCCGCTGGCACAGCTAATACTGCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACATCAAAGCT
GATATCGCTACGAACAAAGATAATATTGCTAAAAAAGCAAACAGTGCCGACGTGTACACCAGAGAAGAGTCTGACAGC
AAATTTGTCAGAATTGATGGTCTGAACGCTACTACCGAAAAATTGGACACACGCTTGGCTTCTGCTGAAAAATCCATT
GCCGATCACGATACTCGCCTGAACGGTTTGGATAAAACAGTGTCAGACCTGCGCAAAGAAACCCGCCAAGGCCTTGCA
GAACAAGCCGCGCTCTCCGGTCTGTTCCAACCTTACAACGTGGGTCTCGAGCACCACCACCACCACCACTGA

751 H*
961-ORF46.1 ATGGCCACAAACGACGACGATGTTAAAAAAGCTGCCACTGTGGCCATTGCTGCTGCCTACAACAATGGCCAAGAAATC
AACGGTTTCAAAGCTGGAGAGACCATCTACGACATTGATGAAGACGGCACAATTACCAAAAAAGACGCAACTGCAGCC
GATGTTGAAGCCGACGACTTTAAAGGTCTGGGTCTGAAAAAAGTCGTGACTAACCTGACCAAAACCGTCAATGAAAAC
AAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAATCTGAAATAGAAAAGTTAACAACCAAGTTAGCAGACACTGAT
GCCGCTTTAGCAGATACTGATGCCGCTCTGGATGCAACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACA
TTTGCTGAAGAGACTAAGACAAATATCGTAAAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCAT
GCCGAAGCATTCAACGATATCGCCGATTCATTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAAT
GAAGCCAAACAGACGGCCGAAGAAACCAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAA
GCCGAAGCTGCCGCTGGCACAGCTAATACTGCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACATCAAA
GCTGATATCGCTACGAACAAAGATAATATTGCTAAAAAAGCAAACAGTGCCGACGTGTACACCAGAGAAGAGTCTGAC
AGCAAATTTGTCAGAATTGATGGTCTGAACGCTACTACCGAAAAATTGGACACACGCTTGGCTTCTGCTGAAAAATCC
ATTGCCGATCACGATACTCGCCTGAACGGTTTGGATAAAACAGTGTCAGACCTGCGCAAAGAAACCCGCCAAGGCCTT
GCAGAACAAGCCGCGCTCTCCGGTCTGTTCCAACCTTACAACGTGGGTCGGTTCAATGTAACGGCTGCAGTCGGCGGC
TACAAATCCGAATCGGCAGTCGCCATCGGTACCGGCTTCCGCTTTACCGAAAACTTTGCCGCCAAAGCAGGCGTGGCA
GTCGGCACTTCGTCCGGTTCTTCCGCAGCCTACCATGTCGGCGTCAATTACGAGTGGGGATCCGGAGGAGGAGGATCA
GATTTGGCAAACGATTCTTTTATCCGGCAGGTTCTCGACCGTCAGCATTTCGAACCCGACGGGAAATACCACCTATTC
GGCAGCAGGGGGGAACTTGCCGAGCGCAGCGGCCATATCGGATTGGGAAAAATACAAAGCCATCAGTTGGGCAACCTG
ATGATTCAACAGGCGGCCATTAAAGGAAATATCGGCTACATTGTCCGCTTTTCCGATCACGGGCACGAAGTCCATTCC
CCCTTCGACAACCATGCCTCACATTCCGATTCTGATGAAGCCGGTAGTCCCGTTGACGGATTTAGCCTTTACCGCATC
CATTGGGACGGATACGAACACCATCCCGCCGACGGCTATGACGGGCCACAGGGCGGCGGCTATCCCGCTCCCAAAGGC
GCGAGGGATATATACAGCTACGACATAAAAGGCGTTGCCCAAAATATCCGCCTCAACCTGACCGACAACCGCAGCACC
GGACAACGGCTTGCCGACCGTTTCCACAATGCCGGTAGTATGCTGACGCAAGGAGTAGGCGACGGATTCAAACGCGCC
ACCCGATACAGCCCCGAGCTGGACAGATCGGGCAATGCCGCCGAAGCCTTCAACGGCACTGCAGATATCGTTAAAAAC
ATCATCGGCGCGGCAGGAGAAATTGTCGGCGCAGGCGATGCCGTGCAGGGCATAAGCGAAGGCTCAAACATTGCTGTC
ATGCACGGCTTGGGTCTGCTTTCCACCGAAAACAAGATGGCGCGCATCAACGATTTGGCAGATATGGCGCAACTCAAA
GACTATGCCGCAGCAGCCATCCGCGATTGGGCAGTCCAAAACCCCAATGCCGCACAAGGCATAGAAGCCGTCAGCAAT
ATCTTTATGGCAGCCATCCCCATCAAAGGGATTGGAGCTGTTCGGGGAAAATACGGCTTGGGCGGCATCACGGCACAT
CCTATCAAGCGGTCGCAGATGGGCGCGATCGCATTGCCGAAAGGGAAATCCGCCGTCAGCGACAATTTTGCCGATGCG
GCATACGCCAAATACCCGTCCCCTTACCATTCCCGAAATATCCGTTCAAACTTGGAGCAGCGTTACGGCAAAGAAAAC
ATCACCTCCTCAACCGTGCCGCCGTCAAACGGCAAAAATGTCAAACTGGCAGACCAACGCCACCCGAAGACAGGCGTA
CCGTTTGACGGTAAAGGGTTTCCGAATTTTGAGAAGCACGTGAAATATGATACGCTCGAGCACCACCACCACCACCAC
TGA

801 HHHHHH*

ATGGCCACAAACGACGACGATGTTAAAAAAGCTGCCACTGTGGCCATTGCTGCTGCCTACAACAATGGCCAAGAAATC
AACGGTTTCAAAGCTGGAGAGACCATCTACGACATTGATGAAGACGGCACAATTACCAAAAAAGACGCAACTGCAGCC
GATGTTGAAGCCGACGACTTTAAAGGTCTGGGTCTGAAAAAAGTCGTGACTAACCTGACCAAAACCGTCAATGAAAAC
AAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAATCTGAAATAGAAAAGTTAACAACCAAGTTAGCAGACACTGAT
GCCGCTTTAGCAGATACTGATGCCGCTCTGGATGCAACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACA
TTTGCTGAAGAGACTAAGACAAATATCGTAAAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCAT
GCCGAAGCATTCAACGATATCGCCGATTCATTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAAT
GAAGCCAAACAGACGGCCGAAGAAACCAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAA
GCCGAAGCTGCCGCTGGCACAGCTAATACTGCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACATCAAA
GCTGATATCGCTACGAACAAAGATAATATTGCTAAAAAAGCAAACAGTGCCGACGTGTACACCAGAGAAGAGTCTGAC
AGCAAATTTGTCAGAATTGATGGTCTGAACGCTACTACCGAAAAATTGGACACACGCTTGGCTTCTGCTGAAAAATCC
ATTGCCGATCACGATACTCGCCTGAACGGTTTGGATAAAACAGTGTCAGACCTGCGCAAAGAAACCCGCCAAGGCCTT
GCAGAACAAGCCGCGCTCTCCGGTCTGTTCCAACCTTACAACGTGGGTCGGTTCAATGTAACGGCTGCAGTCGGCGGC
TACAAATCCGAATCGGCAGTCGCCATCGGTACCGGCTTCCGCTTTACCGAAAACTTTGCCGCCAAAGCAGGCGTGGCA
GTCGGCACTTCGTCCGGTTCTTCCGCAGCCTACCATGTCGGCGTCAATTACGAGTGGGGATCCGGAGGGGGTGGTGTC
GCCGCCGACATCGGTGCGGGGCTTGCCGATGCACTAACCGCACCGCTCGACCATAAAGACAAAGGTTTGCAGTCTTTG
ACGCTGGATCAGTCCGTCAGGAAAAACGAGAAACTGAAGCTGGCGGCACAAGGTGCGGAAAAAACTTATGGAAACGGT
GACAGCCTCAATACGGGCAAATTGAAGAACGACAAGGTCAGCCGTTTCGACTTTATCCGCCAAATCGAAGTGGACGGG
CAGCTCATTACCTTGGAGAGTGGAGAGTTCCAAGTATACAAACAAAGCCATTCCGCCTTAACCGCCTTTCAGACCGAG
CAAATACAAGATTCGGAGCATTCCGGGAAGATGGTTGCGAAACGCCAGTTCAGAATCGGCGACATAGCGGGCGAACAT
ACATCTTTTGACAAGCTTCCCGAAGGCGGCAGGGCGACATATCGCGGGACGGCGTTCGGTTCAGACGATGCCGGCGGA
AAACTGACCTACACCATAGATTTCGCCGCCAAGCAGGGAAACGGCAAAATCGAACATTTGAAATCGCCAGAACTCAAT
GTCGACCTGGCCGCCGCCGATATCAAGCCGGATGGAAAACGCCATGCCGTCATCAGCGGTTCCGTCCTTTACAACCAA
GCCGAGAAAGGCAGTTACTCCCTCGGTATCTTTGGCGGAAAAGCCCAGGAAGTTGCCGGCAGCGCGGAAGTGAAAACC
GTAAACGGCATACGCCATATCGGCCTTGCCGCCAAGCAACTCGAGCACCACCACCACCACCACTGA

601 KGSYSLGIFG GKAQEVAGSA EVKTVNGIRH IGLAAKQLEH HHHHH*

ATGGCCACAAACGACGACGATGTTAAAAAAGCTGCCACTGTGGCCATTGCTGCTGCCTACAACAATGGCCAAGAAATC
AACGGTTTCAAAGCTGGAGAGACCATCTACGACATTGATGAAGACGGCACAATTACCAAAAAAGACGCAACTGCAGCC
GATGTTGAAGCCGACGACTTTAAAGGTCTGGGTCTGAAAAAAGTCGTGACTAACCTGACCAAAACCGTCAATGAAAAC
AAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAATCTGAAATAGAAAAGTTAACAACCAAGTTAGCAGACACTGAT
GCCGCTTTAGCAGATACTGATGCCGCTCTGGATGCAACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACA
TTTGCTGAAGAGACTAAGACAAATATCGTAAAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCAT
GCCGAAGCATTCAACGATATCGCCGATTCATTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAAT
GAAGCCAAACAGACGGCCGAAGAAACCAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAA
GCCGAAGCTGCCGCTGGCACAGCTAATACTGCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACATCAAA
GCTGATATCGCTACGAACAAAGATAATATTGCTAAAAAAGCAAACAGTGCCGACGTGTACACCAGAGAAGAGTCTGAC
AGCAAATTTGTCAGAATTGATGGTCTGAACGCTACTACCGAAAAATTGGACACACGCTTGGCTTCTGCTGAAAAATCC
ATTGCCGATCACGATACTCGCCTGAACGGTTTGGATAAAACAGTGTCAGACCTGCGCAAAGAAACCCGCCAAGGCCTT
GCAGAACAAGCCGCGCTCTCCGGTCTGTTCCAACCTTACAACGTGGGTCGGTTCAATGTAACGGCTGCAGTCGGCGGC
TACAAATCCGAATCGGCAGTCGCCATCGGTACCGGCTTCCGCTTTACCGAAAACTTTGCCGCCAAAGCAGGCGTGGCA
GTCGGCACTTCGTCCGGTTCTTCCGCAGCCTACCATGTCGGCGTCAATTACGAGTGGGGATCCGGCGGAGGCGGCACT
TCTGCGCCCGACTTCAATGCAGGCGGTACCGGTATCGGCAGCAACAGCAGAGCAACAACAGCGAAATCAGCAGCAGTA
TCTTACGCCGGTATCAAGAACGAAATGTGCAAAGACAGAAGCATGCTCTGTGCCGGTCGGGATGACGTTGCGGTTACA
GACAGGGATGCCAAAATCAATGCCCCCCCCCCGAATCTGCATACCGGAGACTTTCCAAACCCAAATGACGCATACAAG
AATTTGATCAACCTCAAACCTGCAATTGAAGCAGGCTATACAGGACGCGGGGTAGAGGTAGGTATCGTCGACACAGGC
GAATCCGTCGGCAGCATATCCTTTCCCGAACTGTATGGCAGAAAAGAACACGGCTATAACGAAAATTACAAAAACTAT
ACGGCGTATATGCGGAAGGAAGCGCCTGAAGACGGAGGCGGTAAAGACATTGAAGCTTCTTTCGACGATGAGGCCGTT
ATAGAGACTGAAGCAAAGCCGACGGATATCCGCCACGTAAAAGAAATCGGACACATCGATTTGGTCTCCCATATTATT
GGCGGGCGTTCCGTGGACGGCAGACCTGCAGGCGGTATTGCGCCCGATGCGACGCTACACATAATGAATACGAATGAT
GAAACCAAGAACGAAATGATGGTTGCAGCCATCCGCAATGCATGGGTCAAGCTGGGCGAACGTGGCGTGCGCATCGTC
AATAACAGTTTTGGAACAACATCGAGGGCAGGCACTGCCGACCTTTTCCAAATAGCCAATTCGGAGGAGCAGTACCGC
CAAGCGTTGCTCGACTATTCCGGCGGTGATAAAACAGACGAGGGTATCCGCCTGATGCAACAGAGCGATTACGGCAAC
CTGTCCTACCACATCCGTAATAAAAACATGCTTTTCATCTTTTCGACAGGCAATGACGCACAAGCTCAGCCCAACACA
TATGCCCTATTGCCATTTTATGAAAAAGACGCTCAAAAAGGCATTATCACAGTCGCAGGCGTAGACCGCAGTGGAGAA
AAGTTCAAACGGGAAATGTATGGAGAACCGGGTACAGAACCGCTTGAGTATGGCTCCAACCATTGCGGAATTACTGCC
ATGTGGTGCCTGTCGGCACCCTATGAAGCAAGCGTCCGTTTCACCCGTACAAACCCGATTCAAATTGCCGGAACATCC
TTTTCCGCACCCATCGTAACCGGCACGGCGGCTCTGCTGCTGCAGAAATACCCGTGGATGAGCAACGACAACCTGCGT
ACCACGTTGCTGACGACGGCTCAGGACATCGGTGCAGTCGGCGTGGACAGCAAGTTCGGCTGGGGACTGCTGGATGCG
GGTAAGGCCATGAACGGACCCGCGTCCTTTCCGTTCGGCGACTTTACCGCCGATACGAAAGGTACATCCGATATTGCC
TACTCCTTCCGTAACGACATTTCAGGCACGGGCGGCCTGATCAAAAAAGGCGGCAGCCAACTGCAACTGCACGGCAAC
AACACCTATACGGGCAAAACCATTATCGAAGGCGGTTCGCTGGTGTTGTACGGCAACAACAAATCGGATATGCGCGTC
GAAACCAAAGGTGCGCTGATTTATAACGGGGCGGCATCCGGCGGCAGCCTGAACAGCGACGGCATTGTCTATCTGGCA
GATACCGACCAATCCGGCGCAAACGAAACCGTACACATCAAAGGCAGTCTGCAGCTGGACGGCAAAGGTACGCTGTAC
ACACGTTTGGGCAAACTGCTGAAAGTGGACGGTACGGCGATTATCGGCGGCAAGCTGTACATGTCGGCACGCGGCAAG
GGGGCAGGCTATCTCAACAGTACCGGACGACGTGTTCCCTTCCTGAGTGCCGCCAAAATCGGGCAGGATTATTCTTTC
TTCACAAACATCGAAACCGACGGCGGCCTGCTGGCTTCCCTCGACAGCGTCGAAAAAACAGCGGGCAGTGAAGGCGAC
ACGCTGTCCTATTATGTCCGTCGCGGCAATGCGGCACGGACTGCTTCGGCAGCGGCACATTCCGCGCCCGCCGGTCTG
AAACACGCCGTAGAACAGGGCGGCAGCAATCTGGAAAACCTGATGGTCGAACTGGATGCCTCCGAATCATCCGCAACA
CCCGAGACGGTTGAAACTGCGGCAGCCGACCGCACAGATATGCCGGGCATCCGCCCCTACGGCGCAACTTTCCGCGCA
GCGGCAGCCGTACAGCATGCGAATGCCGCCGACGGTGTACGCATCTTCAACAGTCTCGCCGCTACCGTCTATGCCGAC
AGTACCGCCGCCCATGCCGATATGCAGGGACGCCGCCTGAAAGCCGTATCGGACGGGTTGGACCACAACGGCACGGGT
CTGCGCGTCATCGCGCAAACCCAACAGGACGGTGGAACGTGGGAACAGGGCGGTGTTGAAGGCAAAATGCGCGGCAGT
ACCCAAACCGTCGGCATTGCCGCGAAAACCGGCGAAAATACGACAGCAGCCGCCACACTGGGCATGGGACGCAGCACA
TGGAGCGAAAACAGTGCAAATGCAAAAACCGACAGCATTAGTCTGTTTGCAGGCATACGGCACGATGCGGGCGATATC
GGCTATCTCAAAGGCCTGTTCTCCTACGGACGCTACAAAAACAGCATCAGCCGCAGCACCGGTGCGGACGAACATGCG
GAAGGCAGCGTCAACGGCACGCTGATGCAGCTGGGCGCACTGGGCGGTGTCAACGTTCCGTTTGCCGCAACGGGAGAT
TTGACGGTCGAAGGCGGTCTGCGCTACGACCTGCTCAAACAGGATGCATTCGCCGAAAAAGGCAGTGCTTTGGGCTGG
AGCGGCAACAGCCTCACTGAAGGCACGCTGGTCGGACTCGCGGGTCTGAAGCTGTCGCAACCCTTGAGCGATAAAGCC
GTCCTGTTTGCAACGGCGGGCGTGGAACGCGACCTGAACGGACGCGACTACACGGTAACGGGCGGCTTTACCGGCGCG
ACTGCAGCAACCGGCAAGACGGGGGCACGCAATATGCCGCACACCCGTCTGGTTGCCGGCCTGGGCGCGGATGTCGAA
TTCGGCAACGGCTGGAACGGCTTGGCACGTTACAGCTACGCCGGTTCCAAACAGTACGGCAACCACAGCGGACGAGTC
GGCGTAGGCTACCGGTTCCTCGAGCACCACCACCACCACCACTGA

1401 ADVEFGNGWN GLARYSYAGS KQYGNHSGRV GVGYRFLEHH HHHH*

961c-ORF46.1 ATGGCCACAAACGACGACGATGTTAAAAAAGCTGCCACTGTGGCCATTGCTGCTGCCTACAACAATGGCCAAGAAATC
AACGGTTTCAAAGCTGGAGAGACCATCTACGACATTGATGAAGACGGCACAATTACCAAAAAAGACGCAACTGCAGCC
GATGTTGAAGCCGACGACTTTAAAGGTCTGGGTCTGAAAAAAGTCGTGACTAACCTGACCAAAACCGTCAATGAAAAC
AAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAATCTGAAATAGAAAAGTTAACAACCAAGTTAGCAGACACTGAT
GCCGCTTTAGCAGATACTGATGCCGCTCTGGATGCAACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACA
TTTGCTGAAGAGACTAAGACAAATATCGTAAAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCAT
GCCGAAGCATTCAACGATATCGCCGATTCATTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAAT
GAAGCCAAACAGACGGCCGAAGAAACCAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAA
GCCGAAGCTGCCGCTGGCACAGCTAATACTGCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACATCAAA
GCTGATATCGCTACGAACAAAGATAATATTGCTAAAAAAGCAAACAGTGCCGACGTGTACACCAGAGAAGAGTCTGAC
AGCAAATTTGTCAGAATTGATGGTCTGAACGCTACTACCGAAAAATTGGACACACGCTTGGCTTCTGCTGAAAAATCC
ATTGCCGATCACGATACTCGCCTGAACGGTTTGGATAAAACAGTGTCAGACCTGCGCAAAGAAACCCGCCAAGGCCTT
GCAGAACAAGCCGCGCTCTCCGGTCTGTTCCAACCTTACAACGTGGGTGGATCCGGAGGAGGAGGATCAGATTTGGCA
AACGATTCTTTTATCCGGCAGGTTCTCGACCGTCAGCATTTCGAACCCGACGGGAAATACCACCTATTCGGCAGCAGG
GGGGAACTTGCCGAGCGCAGCGGCCATATCGGATTGGGAAAAATACAAAGCCATCAGTTGGGCAACCTGATGATTCAA
CAGGCGGCCATTAAAGGAAATATCGGCTACATTGTCCGCTTTTCCGATCACGGGCACGAAGTCCATTCCCCCTTCGAC
AACCATGCCTCACATTCCGATTCTGATGAAGCCGGTAGTCCCGTTGACGGATTTAGCCTTTACCGCATCCATTGGGAC
GGATACGAACACCATCCCGCCGACGGCTATGACGGGCCACAGGGCGGCGGCTATCCCGCTCCCAAAGGCGCGAGGGAT
ATATACAGCTACGACATAAAAGGCGTTGCCCAAAATATCCGCCTCAACCTGACCGACAACCGCAGCACCGGACAACGG
CTTGCCGACCGTTTCCACAATGCCGGTAGTATGCTGACGCAAGGAGTAGGCGACGGATTCAAACGCGCCACCCGATAC
AGCCCCGAGCTGGACAGATCGGGCAATGCCGCCGAAGCCTTCAACGGCACTGCAGATATCGTTAAAAACATCATCGGC
GCGGCAGGAGAAATTGTCGGCGCAGGCGATGCCGTGCAGGGCATAAGCGAAGGCTCAAACATTGCTGTCATGCACGGC
TTGGGTCTGCTTTCCACCGAAAACAAGATGGCGCGCATCAACGATTTGGCAGATATGGCGCAACTCAAAGACTATGCC
GCAGCAGCCATCCGCGATTGGGCAGTCCAAAACCCCAATGCCGCACAAGGCATAGAAGCCGTCAGCAATATCTTTATG
GCAGCCATCCCCATCAAAGGGATTGGAGCTGTTCGGGGAAAATACGGCTTGGGCGGCATCACGGCACATCCTATCAAG
CGGTCGCAGATGGGCGCGATCGCATTGCCGAAAGGGAAATCCGCCGTCAGCGACAATTTTGCCGATGCGGCATACGCC
AAATACCCGTCCCCTTACCATTCCCGAAATATCCGTTCAAACTTGGAGCAGCGTTACGGCAAAGAAAACATCACCTCC
TCAACCGTGCCGCCGTCAAACGGCAAAAATGTCAAACTGGCAGACCAACGCCACCCGAAGACAGGCGTACCGTTTGAC
GGTAAAGGGTTTCCGAATTTTGAGAAGCACGTGAAATATGATACGCTCGAGCACCACCACCACCACCACTGA

751 H*

961c-741 ATGGCCACAAACGACGACGATGTTAAAAAAGCTGCCACTGTGGCCATTGCTGCTGCCTACAACAATGGCCAAGAAATC
AACGGTTTCAAAGCTGGAGAGACCATCTACGACATTGATGAAGACGGCACAATTACCAAAAAAGACGCAACTGCAGCC
GATGTTGAAGCCGACGACTTTAAAGGTCTGGGTCTGAAAAAAGTCGTGACTAACCTGACCAAAACCGTCAATGAAAAC
AAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAATCTGAAATAGAAAAGTTAACAACCAAGTTAGCAGACACTGAT
GCCGCTTTAGCAGATACTGATGCCGCTCTGGATGCAACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACA
TTTGCTGAAGAGACTAAGACAAATATCGTAAAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCAT
GCCGAAGCATTCAACGATATCGCCGATTCATTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAAT
GAAGCCAAACAGACGGCCGAAGAAACCAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAA
GCCGAAGCTGCCGCTGGCACAGCTAATACTGCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACATCAAA
GCTGATATCGCTACGAACAAAGATAATATTGCTAAAAAAGCAAACAGTGCCGACGTGTACACCAGAGAAGAGTCTGAC
AGCAAATTTGTCAGAATTGATGGTCTGAACGCTACTACCGAAAAATTGGACACACGCTTGGCTTCTGCTGAAAAATCC
ATTGCCGATCACGATACTCGCCTGAACGGTTTGGATAAAACAGTGTCAGACCTGCGCAAAGAAACCCGCCAAGGCCTT
GCAGAACAAGCCGCGCTCTCCGGTCTGTTCCAACCTTACAACGTGGGTGGATCCGGAGGGGGTGGTGTCGCCGCCGAC
ATCGGTGCGGGGCTTGCCGATGCACTAACCGCACCGCTCGACCATAAAGACAAAGGTTTGCAGTCTTTGACGCTGGAT
CAGTCCGTCAGGAAAAACGAGAAACTGAAGCTGGCGGCACAAGGTGCGGAAAAAACTTATGGAAACGGTGACAGCCTC
AATACGGGCAAATTGAAGAACGACAAGGTCAGCCGTTTCGACTTTATCCGCCAAATCGAAGTGGACGGGCAGCTCATT
ACCTTGGAGAGTGGAGAGTTCCAAGTATACAAACAAAGCCATTCCGCCTTAACCGCCTTTCAGACCGAGCAAATACAA
GATTCGGAGCATTCCGGGAAGATGGTTGCGAAACGCCAGTTCAGAATCGGCGACATAGCGGGCGAACATACATCTTTT
GACAAGCTTCCCGAAGGCGGCAGGGCGACATATCGCGGGACGGCGTTCGGTTCAGACGATGCCGGCGGAAAACTGACC
TACACCATAGATTTCGCCGCCAAGCAGGGAAACGGCAAAATCGAACATTTGAAATCGCCAGAACTCAATGTCGACCTG
GCCGCCGCCGATATCAAGCCGGATGGAAAACGCCATGCCGTCATCAGCGGTTCCGTCCTTTACAACCAAGCCGAGAAA
GGCAGTTACTCCCTCGGTATCTTTGGCGGAAAAGCCCAGGAAGTTGCCGGCAGCGCGGAAGTGAAAACCGTAAACGGC
ATACGCCATATCGGCCTTGCCGCCAAGCAACTCGAGCACCACCACCACCACCACTGA
961c-983 ATGGCCACAAACGACGACGATGTTAAAAAAGCTGCCACTGTGGCCATTGCTGCTGCCTACAACAATGGCCAAGAAATC
AACGGTTTCAAAGCTGGAGAGACCATCTACGACATTGATGAAGACGGCACAATTACCAAAAAAGACGCAACTGCAGCC
GATGTTGAAGCCGACGACTTTAAAGGTCTGGGTCTGAAAAAAGTCGTGACTAACCTGACCAAAACCGTCAATGAAAAC
AAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAATCTGAAATAGAAAAGTTAACAACCAAGTTAGCAGACACTGAT
GCCGCTTTAGCAGATACTGATGCCGCTCTGGATGCAACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACA
TTTGCTGAAGAGACTAAGACAAATATCGTAAAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCAT
GCCGAAGCATTCAACGATATCGCCGATTCATTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAAT
GAAGCCAAACAGACGGCCGAAGAAACCAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAA
GCCGAAGCTGCCGCTGGCACAGCTAATACTGCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACATCAAA
GCTGATATCGCTACGAACAAAGATAATATTGCTAAAAAAGCAAACAGTGCCGACGTGTACACCAGAGAAGAGTCTGAC
AGCAAATTTGTCAGAATTGATGGTCTGAACGCTACTACCGAAAAATTGGACACACGCTTGGCTTCTGCTGAAAAATCC
ATTGCCGATCACGATACTCGCCTGAACGGTTTGGATAAAACAGTGTCAGACCTGCGCAAAGAAACCCGCCAAGGCCTT
GCAGAACAAGCCGCGCTCTCCGGTCTGTTCCAACCTTACAACGTGGGTGGATCCGGCGGAGGCGGCACTTCTGCGCCC
GACTTCAATGCAGGCGGTACCGGTATCGGCAGCAACAGCAGAGCAACAACAGCGAAATCAGCAGCAGTATCTTACGCC
GGTATCAAGAACGAAATGTGCAAAGACAGAAGCATGCTCTGTGCCGGTCGGGATGACGTTGCGGTTACAGACAGGGAT
GCCAAAATCAATGCCCCCCCCCCGAATCTGCATACCGGAGACTTTCCAAACCCAAATGACGCATACAAGAATTTGATC
AACCTCAAACCTGCAATTGAAGCAGGCTATACAGGACGCGGGGTAGAGGTAGGTATCGTCGACACAGGCGAATCCGTC
GGCAGCATATCCTTTCCCGAACTGTATGGCAGAAAAGAACACGGCTATAACGAAAATTACAAAAACTATACGGCGTAT
ATGCGGAAGGAAGCGCCTGAAGACGGAGGCGGTAAAGACATTGAAGCTTCTTTCGACGATGAGGCCGTTATAGAGACT
GAAGCAAAGCCGACGGATATCCGCCACGTAAAAGAAATCGGACACATCGATTTGGTCTCCCATATTATTGGCGGGCGT
TCCGTGGACGGCAGACCTGCAGGCGGTATTGCGCCCGATGCGACGCTACACATAATGAATACGAATGATGAAACCAAG
AACGAAATGATGGTTGCAGCCATCCGCAATGCATGGGTCAAGCTGGGCGAACGTGGCGTGCGCATCGTCAATAACAGT
TTTGGAACAACATCGAGGGCAGGCACTGCCGACCTTTTCCAAATAGCCAATTCGGAGGAGCAGTACCGCCAAGCGTTG
CTCGACTATTCCGGCGGTGATAAAACAGACGAGGGTATCCGCCTGATGCAACAGAGCGATTACGGCAACCTGTCCTAC
CACATCCGTAATAAAAACATGCTTTTCATCTTTTCGACAGGCAATGACGCACAAGCTCAGCCCAACACATATGCCCTA
TTGCCATTTTATGAAAAAGACGCTCAAAAAGGCATTATCACAGTCGCAGGCGTAGACCGCAGTGGAGAAAAGTTCAAA
CGGGAAATGTATGGAGAACCGGGTACAGAACCGCTTGAGTATGGCTCCAACCATTGCGGAATTACTGCCATGTGGTGC
CTGTCGGCACCCTATGAAGCAAGCGTCCGTTTCACCCGTACAAACCCGATTCAAATTGCCGGAACATCCTTTTCCGCA
CCCATCGTAACCGGCACGGCGGCTCTGCTGCTGCAGAAATACCCGTGGATGAGCAACGACAACCTGCGTACCACGTTG
CTGACGACGGCTCAGGACATCGGTGCAGTCGGCGTGGACAGCAAGTTCGGCTGGGGACTGCTGGATGCGGGTAAGGCC
ATGAACGGACCCGCGTCCTTTCCGTTCGGCGACTTTACCGCCGATACGAAAGGTACATCCGATATTGCCTACTCCTTC
CGTAACGACATTTCAGGCACGGGCGGCCTGATCAAAAAAGGCGGCAGCCAACTGCAACTGCACGGCAACAACACCTAT
ACGGGCAAAACCATTATCGAAGGCGGTTCGCTGGTGTTGTACGGCAACAACAAATCGGATATGCGCGTCGAAACCAAA
GGTGCGCTGATTTATAACGGGGCGGCATCCGGCGGCAGCCTGAACAGCGACGGCATTGTCTATCTGGCAGATACCGAC
CAATCCGGCGCAAACGAAACCGTACACATCAAAGGCAGTCTGCAGCTGGACGGCAAAGGTACGCTGTACACACGTTTG
GGCAAACTGCTGAAAGTGGACGGTACGGCGATTATCGGCGGCAAGCTGTACATGTCGGCACGCGGCAAGGGGGCAGGC
TATCTCAACAGTACCGGACGACGTGTTCCCTTCCTGAGTGCCGCCAAAATCGGGCAGGATTATTCTTTCTTCACAAAC
ATCGAAACCGACGGCGGCCTGCTGGCTTCCCTCGACAGCGTCGAAAAAACAGCGGGCAGTGAAGGCGACACGCTGTCC
TATTATGTCCGTCGCGGCAATGCGGCACGGACTGCTTCGGCAGCGGCACATTCCGCGCCCGCCGGTCTGAAACACGCC
GTAGAACAGGGCGGCAGCAATCTGGAAAACCTGATGGTCGAACTGGATGCCTCCGAATCATCCGCAACACCCGAGACG
GTTGAAACTGCGGCAGCCGACCGCACAGATATGCCGGGCATCCGCCCCTACGGCGCAACTTTCCGCGCAGCGGCAGCC
GTACAGCATGCGAATGCCGCCGACGGTGTACGCATCTTCAACAGTCTCGCCGCTACCGTCTATGCCGACAGTACCGCC
GCCCATGCCGATATGCAGGGACGCCGCCTGAAAGCCGTATCGGACGGGTTGGACCACAACGGCACGGGTCTGCGCGTC
ATCGCGCAAACCCAACAGGACGGTGGAACGTGGGAACAGGGCGGTGTTGAAGGCAAAATGCGCGGCAGTACCCAAACC
GTCGGCATTGCCGCGAAAACCGGCGAAAATACGACAGCAGCCGCCACACTGGGCATGGGACGCAGCACATGGAGCGAA
AACAGTGCAAATGCAAAAACCGACAGCATTAGTCTGTTTGCAGGCATACGGCACGATGCGGGCGATATCGGCTATCTC
AAAGGCCTGTTCTCCTACGGACGCTACAAAAACAGCATCAGCCGCAGCACCGGTGCGGACGAACATGCGGAAGGCAGC
GTCAACGGCACGCTGATGCAGCTGGGCGCACTGGGCGGTGTCAACGTTCCGTTTGCCGCAACGGGAGATTTGACGGTC
GAAGGCGGTCTGCGCTACGACCTGCTCAAACAGGATGCATTCGCCGAAAAAGGCAGTGCTTTGGGCTGGAGCGGCAAC
AGCCTCACTGAAGGCACGCTGGTCGGACTCGCGGGTCTGAAGCTGTCGCAACCCTTGAGCGATAAAGCCGTCCTGTTT
GCAACGGCGGGCGTGGAACGCGACCTGAACGGACGCGACTACACGGTAACGGGCGGCTTTACCGGCGCGACTGCAGCA
ACCGGCAAGACGGGGGCACGCAATATGCCGCACACCCGTCTGGTTGCCGGCCTGGGCGCGGATGTCGAATTCGGCAAC
GGCTGGAACGGCTTGGCACGTTACAGCTACGCCGGTTCCAAACAGTACGGCAACCACAGCGGACGAGTCGGCGTAGGC
TACCGGTTCCTCGAGCACCACCACCACCACCACTGA
1351 GNGWNGLARY SYAGSKQYGN HSGRVGVGYR FLEHHHHHH*

961cL-ORF46.1 ATGAAACACTTTCCATCCAAAGTACTGACCACAGCCATCCTTGCCACTTTCTGTAGCGGCGCACTGGCAGCCACAAAC
GACGACGATGTTAAAAAAGCTGCCACTGTGGCCATTGCTGCTGCCTACAACAATGGCCAAGAAATCAACGGTTTCAAA
GCTGGAGAGACCATCTACGACATTGATGAAGACGGCACAATTACCAAAAAAGACGCAACTGCAGCCGATGTTGAAGCC
GACGACTTTAAAGGTCTGGGTCTGAAAAAAGTCGTGACTAACCTGACCAAAACCGTCAATGAAAACAAACAAAACGTC
GATGCCAAAGTAAAAGCTGCAGAATCTGAAATAGAAAAGTTAACAACCAAGTTAGCAGACACTGATGCCGCTTTAGCA
GATACTGATGCCGCTCTGGATGCAACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACATTTGCTGAAGAG
ACTAAGACAAATATCGTAAAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCATGCCGAAGCATTC
AACGATATCGCCGATTCATTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAATGAAGCCAAACAG
ACGGCCGAAGAAACCAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAAGCCGAAGCTGCC
GCTGGCACAGCTAATACTGCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACATCAAAGCTGATATCGCT
ACGAACAAAGATAATATTGCTAAAAAAGCAAACAGTGCCGACGTGTACACCAGAGAAGAGTCTGACAGCAAATTTGTC
AGAATTGATGGTCTGAACGCTACTACCGAAAAATTGGACACACGCTTGGCTTCTGCTGAAAAATCCATTGCCGATCAC
GATACTCGCCTGAACGGTTTGGATAAAACAGTGTCAGACCTGCGCAAAGAAACCCGCCAAGGCCTTGCAGAACAAGCC
GCGCTCTCCGGTCTGTTCCAACCTTACAACGTGGGTGGATCCGGAGGAGGAGGATCAGATTTGGCAAACGATTCTTTT
ATCCGGCAGGTTCTCGACCGTCAGCATTTCGAACCCGACGGGAAATACCACCTATTCGGCAGCAGGGGGGAACTTGCC
GAGCGCAGCGGCCATATCGGATTGGGAAAAATACAAAGCCATCAGTTGGGCAACCTGATGATTCAACAGGCGGCCATT
AAAGGAAATATCGGCTACATTGTCCGCTTTTCCGATCACGGGCACGAAGTCCATTCCCCCTTCGACAACCATGCCTCA
CATTCCGATTCTGATGAAGCCGGTAGTCCCGTTGACGGATTTAGCCTTTACCGCATCCATTGGGACGGATACGAACAC
CATCCCGCCGACGGCTATGACGGGCCACAGGGCGGCGGCTATCCCGCTCCCAAAGGCGCGAGGGATATATACAGCTAC
GACATAAAAGGCGTTGCCCAAAATATCCGCCTCAACCTGACCGACAACCGCAGCACCGGACAACGGCTTGCCGACCGT
TTCCACAATGCCGGTAGTATGCTGACGCAAGGAGTAGGCGACGGATTCAAACGCGCCACCCGATACAGCCCCGAGCTG
GACAGATCGGGCAATGCCGCCGAAGCCTTCAACGGCACTGCAGATATCGTTAAAAACATCATCGGCGCGGCAGGAGAA
ATTGTCGGCGCAGGCGATGCCGTGCAGGGCATAAGCGAAGGCTCAAACATTGCTGTCATGCACGGCTTGGGTCTGCTT
TCCACCGAAAACAAGATGGCGCGCATCAACGATTTGGCAGATATGGCGCAACTCAAAGACTATGCCGCAGCAGCCATC
CGCGATTGGGCAGTCCAAAACCCCAATGCCGCACAAGGCATAGAAGCCGTCAGCAATATCTTTATGGCAGCCATCCCC
ATCAAAGGGATTGGAGCTGTTCGGGGAAAATACGGCTTGGGCGGCATCACGGCACATCCTATCAAGCGGTCGCAGATG
GGCGCGATCGCATTGCCGAAAGGGAAATCCGCCGTCAGCGACAATTTTGCCGATGCGGCATACGCCAAATACCCGTCC
CCTTACCATTCCCGAAATATCCGTTCAAACTTGGAGCAGCGTTACGGCAAAGAAAACATCACCTCCTCAACCGTGCCG
CCGTCAAACGGCAAAAATGTCAAACTGGCAGACCAACGCCACCCGAAGACAGGCGTACCGTTTGACGGTAAAGGGTTT
CCGAATTTTGAGAAGCACGTGAAATATGATACGTAACTCGAG
751 GKGFPNFEKH VKYDT*

961cL-741 ATGAAACACTTTCCATCCAAAGTACTGACCACAGCCATCCTTGCCACTTTCTGTAGCGGCGCACTGGCAGCCACAAAC
GACGACGATGTTAAAAAAGCTGCCACTGTGGCCATTGCTGCTGCCTACAACAATGGCCAAGAAATCAACGGTTTCAAA
GCTGGAGAGACCATCTACGACATTGATGAAGACGGCACAATTACCAAAAAAGACGCAACTGCAGCCGATGTTGAAGCC
GACGACTTTAAAGGTCTGGGTCTGAAAAAAGTCGTGACTAACCTGACCAAAACCGTCAATGAAAACAAACAAAACGTC
GATGCCAAAGTAAAAGCTGCAGAATCTGAAATAGAAAAGTTAACAACCAAGTTAGCAGACACTGATGCCGCTTTAGCA
GATACTGATGCCGCTCTGGATGCAACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACATTTGCTGAAGAG
ACTAAGACAAATATCGTAAAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCATGCCGAAGCATTC
AACGATATCGCCGATTCATTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAATGAAGCCAAACAG
ACGGCCGAAGAAACCAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAAGCCGAAGCTGCC
GCTGGCACAGCTAATACTGCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACATCAAAGCTGATATCGCT
ACGAACAAAGATAATATTGCTAAAAAAGCAAACAGTGCCGACGTGTACACCAGAGAAGAGTCTGACAGCAAATTTGTC
AGAATTGATGGTCTGAACGCTACTACCGAAAAATTGGACACACGCTTGGCTTCTGCTGAAAAATCCATTGCCGATCAC
GATACTCGCCTGAACGGTTTGGATAAAACAGTGTCAGACCTGCGCAAAGAAACCCGCCAAGGCCTTGCAGAACAAGCC
GCGCTCTCCGGTCTGTTCCAACCTTACAACGTGGGTGGATCCGGAGGGGGTGGTGTCGCCGCCGACATCGGTGCGGGG
CTTGCCGATGCACTAACCGCACCGCTCGACCATAAAGACAAAGGTTTGCAGTCTTTGACGCTGGATCAGTCCGTCAGG
AAAAACGAGAAACTGAAGCTGGCGGCACAAGGTGCGGAAAAAACTTATGGAAACGGTGACAGCCTCAATACGGGCAAA
TTGAAGAACGACAAGGTCAGCCGTTTCGACTTTATCCGCCAAATCGAAGTGGACGGGCAGCTCATTACCTTGGAGAGT
GGAGAGTTCCAAGTATACAAACAAAGCCATTCCGCCTTAACCGCCTTTCAGACCGAGCAAATACAAGATTCGGAGCAT
TCCGGGAAGATGGTTGCGAAACGCCAGTTCAGAATCGGCGACATAGCGGGCGAACATACATCTTTTGACAAGCTTCCC
GAAGGCGGCAGGGCGACATATCGCGGGACGGCGTTCGGTTCAGACGATGCCGGCGGAAAACTGACCTACACCATAGAT
TTCGCCGCCAAGCAGGGAAACGGCAAAATCGAACATTTGAAATCGCCAGAACTCAATGTCGACCTGGCCGCCGCCGAT
ATCAAGCCGGATGGAAAACGCCATGCCGTCATCAGCGGTTCCGTCCTTTACAACCAAGCCGAGAAAGGCAGTTACTCC
CTCGGTATCTTTGGCGGAAAAGCCCAGGAAGTTGCCGGCAGCGCGGAAGTGAAAACCGTAAACGGCATACGCCATATC
GGCCTTGCCGCCAAGCAACTCGAGCACCACCACCACCACCACTGA

601 AAKQLEHHHH HH*

961cL-983 ATGAAACACTTTCCATCCAAAGTACTGACCACAGCCATCCTTGCCACTTTCTGTAGCGGCGCACTGGCAGCCACAAAC
GACGACGATGTTAAAAAAGCTGCCACTGTGGCCATTGCTGCTGCCTACAACAATGGCCAAGAAATCAACGGTTTCAAA
GCTGGAGAGACCATCTACGACATTGATGAAGACGGCACAATTACCAAAAAAGACGCAACTGCAGCCGATGTTGAAGCC
GACGACTTTAAAGGTCTGGGTCTGAAAAAAGTCGTGACTAACCTGACCAAAACCGTCAATGAAAACAAACAAAACGTC
GATGCCAAAGTAAAAGCTGCAGAATCTGAAATAGAAAAGTTAACAACCAAGTTAGCAGACACTGATGCCGCTTTAGCA
GATACTGATGCCGCTCTGGATGCAACCACCAACGCCTTGAATAAATTGGGAGAAAATATAACGACATTTGCTGAAGAG
ACTAAGACAAATATCGTAAAAATTGATGAAAAATTAGAAGCCGTGGCTGATACCGTCGACAAGCATGCCGAAGCATTC
AACGATATCGCCGATTCATTGGATGAAACCAACACTAAGGCAGACGAAGCCGTCAAAACCGCCAATGAAGCCAAACAG
ACGGCCGAAGAAACCAAACAAAACGTCGATGCCAAAGTAAAAGCTGCAGAAACTGCAGCAGGCAAAGCCGAAGCTGCC
GCTGGCACAGCTAATACTGCAGCCGACAAGGCCGAAGCTGTCGCTGCAAAAGTTACCGACATCAAAGCTGATATCGCT
ACGAACAAAGATAATATTGCTAAAAAAGCAAACAGTGCCGACGTGTACACCAGAGAAGAGTCTGACAGCAAATTTGTC
AGAATTGATGGTCTGAACGCTACTACCGAAAAATTGGACACACGCTTGGCTTCTGCTGAAAAATCCATTGCCGATCAC
GATACTCGCCTGAACGGTTTGGATAAAACAGTGTCAGACCTGCGCAAAGAAACCCGCCAAGGCCTTGCAGAACAAGCC
GCGCTCTCCGGTCTGTTCCAACCTTACAACGTGGGTGGATCCGGCGGAGGCGGCACTTCTGCGCCCGACTTCAATGCA
GGCGGTACCGGTATCGGCAGCAACAGCAGAGCAACAACAGCGAAATCAGCAGCAGTATCTTACGCCGGTATCAAGAAC
GAAATGTGCAAAGACAGAAGCATGCTCTGTGCCGGTCGGGATGACGTTGCGGTTACAGACAGGGATGCCAAAATCAAT
GCCCCCCCCCCGAATCTGCATACCGGAGACTTTCCAAACCCAAATGACGCATACAAGAATTTGATCAACCTCAAACCT
GCAATTGAAGCAGGCTATACAGGACGCGGGGTAGAGGTAGGTATCGTCGACACAGGCGAATCCGTCGGCAGCATATCC
TTTCCCGAACTGTATGGCAGAAAAGAACACGGCTATAACGAAAATTACAAAAACTATACGGCGTATATGCGGAAGGAA
GCGCCTGAAGACGGAGGCGGTAAAGACATTGAAGCTTCTTTCGACGATGAGGCCGTTATAGAGACTGAAGCAAAGCCG
ACGGATATCCGCCACGTAAAAGAAATCGGACACATCGATTTGGTCTCCCATATTATTGGCGGGCGTTCCGTGGACGGC
AGACCTGCAGGCGGTATTGCGCCCGATGCGACGCTACACATAATGAATACGAATGATGAAACCAAGAACGAAATGATG
GTTGCAGCCATCCGCAATGCATGGGTCAAGCTGGGCGAACGTGGCGTGCGCATCGTCAATAACAGTTTTGGAACAACA
TCGAGGGCAGGCACTGCCGACCTTTTCCAAATAGCCAATTCGGAGGAGCAGTACCGCCAAGCGTTGCTCGACTATTCC
GGCGGTGATAAAACAGACGAGGGTATCCGCCTGATGCAACAGAGCGATTACGGCAACCTGTCCTACCACATCCGTAAT
AAAAACATGCTTTTCATCTTTTCGACAGGCAATGACGCACAAGCTCAGCCCAACACATATGCCCTATTGCCATTTTAT
GAAAAAGACGCTCAAAAAGGCATTATCACAGTCGCAGGCGTAGACCGCAGTGGAGAAAAGTTCAAACGGGAAATGTAT
GGAGAACCGGGTACAGAACCGCTTGAGTATGGCTCCAACCATTGCGGAATTACTGCCATGTGGTGCCTGTCGGCACCC
TATGAAGCAAGCGTCCGTTTCACCCGTACAAACCCGATTCAAATTGCCGGAACATCCTTTTCCGCACCCATCGTAACC
GGCACGGCGGCTCTGCTGCTGCAGAAATACCCGTGGATGAGCAACGACAACCTGCGTACCACGTTGCTGACGACGGCT
CAGGACATCGGTGCAGTCGGCGTGGACAGCAAGTTCGGCTGGGGACTGCTGGATGCGGGTAAGGCCATGAACGGACCC
GCGTCCTTTCCGTTCGGCGACTTTACCGCCGATACGAAAGGTACATCCGATATTGCCTACTCCTTCCGTAACGACATT
TCAGGCACGGGCGGCCTGATCAAA.AAAGGCGGCAGCCAACTGCAACTGCACGGCAACAACACCTATACGGGCAAAACC

ATTATCGAAGGCGGTTCGCTGGTGTTGTACGGCAACAACAAATCGGATATGCGCGTCGAAACCAAAGGTGCGCTGATT
TATAACGGGGCGGCATCCGGCGGCAGCCTGAACAGCGACGGCATTGTCTATCTGGCAGATACCGACCAATCCGGCGCA
AACGAAACCGTACACATCAAAGGCAGTCTGCAGCTGGACGGCAAAGGTACGCTGTACACACGTTTGGGCAAACTGCTG
AAAGTGGACGGTACGGCGATTATCGGCGGCAAGCTGTACATGTCGGCACGCGGCAAGGGGGCAGGCTATCTCAACAGT
ACCGGACGACGTGTTCCCTTCCTGAGTGCCGCCAAAATCGGGCAGGATTATTCTTTCTTCACAAACATCGAAACCGAC
GGCGGCCTGCTGGCTTCCCTCGACAGCGTCGAAAAAACAGCGGGCAGTGAAGGCGACACGCTGTCCTATTATGTCCGT
CGCGGCAATGCGGCACGGACTGCTTCGGCAGCGGCACATTCCGCGCCCGCCGGTCTGAAACACGCCGTAGAACAGGGC
GGCAGCAATCTGGAAAACCTGATGGTCGAACTGGATGCCTCCGAATCATCCGCAACACCCGAGACGGTTGAAACTGCG
GCAGCCGACCGCACAGATATGCCGGGCATCCGCCCCTACGGCGCAACTTTCCGCGCAGCGGCAGCCGTACAGCATGCG
AATGCCGCCGACGGTGTACGCATCTTCAACAGTCTCGCCGCTACCGTCTATGCCGACAGTACCGCCGCCCATGCCGAT
ATGCAGGGACGCCGCCTGAAAGCCGTATCGGACGGGTTGGACCACAACGGCACGGGTCTGCGCGTCATCGCGCAAACC
CAACAGGACGGTGGAACGTGGGAACAGGGCGGTGTTGAAGGCAAAATGCGCGGCAGTACCCAAACCGTCGGCATTGCC
GCGAAAACCGGCGAAAATACGACAGCAGCCGCCACACTGGGCATGGGACGCAGCACATGGAGCGAAAACAGTGCAAAT
GCAAAAACCGACAGCATTAGTCTGTTTGCAGGCATACGGCACGATGCGGGCGATATCGGCTATCTCAAAGGCCTGTTC
TCCTACGGACGCTACAAAAACAGCATCAGCCGCAGCACCGGTGCGGACGAACATGCGGAAGGCAGCGTCAACGGCACG
CTGATGCAGCTGGGCGCACTGGGCGGTGTCAACGTTCCGTTTGCCGCAACGGGAGATTTGACGGTCGAAGGCGGTCTG
CGCTACGACCTGCTCAAACAGGATGCATTCGCCGAAAAAGGCAGTGCTTTGGGCTGGAGCGGCAACAGCCTCACTGAA
GGCACGCTGGTCGGACTCGCGGGTCTGAAGCTGTCGCAACCCTTGAGCGATAAAGCCGTCCTGTTTGCAACGGCGGGC
GTGGAACGCGACCTGAACGGACGCGACTACACGGTAACGGGCGGCTTTACCGGCGCGACTGCAGCAACCGGCAAGACG
GGGGCACGCAATATGCCGCACACCCGTCTGGTTGCCGGCCTGGGCGCGGATGTCGAATTCGGCAACGGCTGGAACGGC
TTGGCACGTTACAGCTACGCCGGTTCCAAACAGTACGGCAACCACAGCGGACGAGTCGGCGTAGGCTACCGGTTCTGA
CTCGAG

1401 YRF*
It will be understood that the invention has been described by way of example only and modifications may be made whilst remaining within the scope and spirit of the invention. For instance, the use of proteins from other strains is envisaged [e.g. see W000/66741 for polymorphic sequences for ORF4, ORF40, ORF46, 225, 235, 287, 519, 726, 919 and 953].

EXPERIMENTAL DETAILS

Cloning strategy and oligonucleotide design Genes coding for antigens of interest were amplified by PCR, using oligonucleotides designed on the basis of the genomic sequence of N. nzeningitidis B MC58.
Genomic DNA
from strain 2996 was always used as a template in PCR reactions, unless otherwise specified, and the amplified fragments were cloned in the expression vector pET2lb+
(Novagen) to express the protein as C-terminal His-tagged product, or in pET-24b+(Novagen) to express the protein in `untagged' form (e.g. AG 287K).

Where a protein was expressed without a fusion partner and with its own leader peptide (if present), amplification of the open reading frame (ATG to STOP codons) was performed.
Where a protein was expressed in `untagged' form, the leader peptide was omitted by designing the 5'-end amplification primer downstream from the predicted leader sequence.
The melting temperature of the primers used in PCR depended on the number and type of hybridising nucleotides in the whole primer, and was determined using the formulae:

Tmi = 4 (G+C)+ 2 (A+T) (tail excluded) Tm2 = 64.9 + 0.41 (% GC) - 600/N (whole primer) The melting temperatures of the selected oligonucleotides were usually 65-70 C
for the whole oligo and 50-60 C for the hybridising region alone.

Oligonucleotides were synthesised using a Perkin Elmer 394 DNA/RNA
Synthesizer, eluted from the columns in 2.0ml NH4OH, and deprotected by 5 hours incubation at 56 C. The oligos were precipitated by addition of 0.3M Na-Acetate and 2 volumes ethanol.
The samples were centrifuged and the pellets resuspended in water.

Sequences Restriction site fu (961)- Fwd CGCGGATCC -GGAGGGGGTGGTGTCG BamHI
741(MC58)-His Rev CCCGCTCGAG-TTGCTTGGCGGCAAGGC XhoI
fu (961 )-983-His Fwd CGCGGATCC - GGCGGAGGCGGCACTT BamHI
Rev CCCGCTCGAG-GAACCGGTAGCCTACG Xhol fu (961)- Orf46.1- Fwd CGCGGATCCGGTGGTGGTGGT- BamHI
His TCAGATTTGGCAAACGATTC
Rev CCCGCTCGAG-CGTATCATATTTCACGTGC XhoI
fu (961 c-L)- Fwd CGCGGATCC -GGAGGGGGTGGTGTCG BamHI
741(MC58) Rev CCCGCTCGAG-TTATTGCTTGGCGGCAAG Xhol fu (961c-L )-983 Fwd CGCGGATCC - GGCGGAGGCGGCACTT BamHI
Rev CCCGCTCGAG-TCAGAACCGGTAGCCTAC XhoI
fu (961c-L)- Fwd CGCGGATCCGGTGGTGGTGGT- BamHI
Orf46.1 TCAGATTTGGCAAACGATTC
Rev CCCGCTCGAG-TTACGTATCATATTTCACGTGC XhoI
fu-(AG287)-919- Fwd CGCGGATCCGGTGGTGGTGGT- BamHI
His CAAAGCAAGAGCATCCAAACC
Rev CCCAAGCTT-TTCGGGCGGTATTCGGGCTTC HindIII
fu-(AG287)-953- Fwd CGCGGATCCGGTGGTGGTGGT- BamHI
His GCCACCTACAAAGTGGAC
Rev GCCCAAGCTT-TTGTTTGGCTGCCTCGAT HindIII
fu-(AG287)-961- Fwd CGCGGATCCGGTGGTGGTGGT-ACAAGCGACGACG BamHI
His Rev GCCCAAGCTT-CCACTCGTAATTGACGCC HindIII
fu-(AG287)- Fwd CGCGGATCCGGTGGTGGTGGT- BamHI
Orf46.1-His TCAGATTTGGCAAACGATTC
Rev CCCAAGCTT-CGTATCATATTTCACGTGC HindIll fu-(AG287-919)- Fwd CCCAAGCTTGGTGGTGGTGGTGGT- HindIII
Orf46.1-His TCAGATTTGGCAAACGATTC
Rev CCCGCTCGAG-CGTATCATATTTCACGTGC XhoI
fu-(AG287- Fwd CCCAAGCTTGGTGGTGGTGGTGGT- HindIII
Orf46.1)-919-His CAAAGCAAGAGCATCCAAACC
Rev CCCGCTCGAG-CGGGCGGTATTCGGGCTT XhoI
fu AG287(394.98)- Fwd CGCGGATCCGCTAGC-CCCGATGTTAAATCGGC NheI
Rev CGGGGATCC-ATCCTGCTCTTTTTTGCCGG BamHI
fu Orfl-(Orf46.1)- Fwd CGCGGATCCGCTAGC-GGACACACTTATTTCGGCATC NheI
His Rev CGCGGATCC-CCAGCGGTAGCCTAATTTGAT
fu (Orfl)-Orf46.1- Fwd CGCGGATCCGGTGGTGGTGGT- BamHI
His TCAGATTTGGCAAACGATTC
Rev CCCAAGCTT-CGTATCATATTTCACGTGC HindIII
fu (919)-Orf46.1- Fwdl GCGGCGTCGACGGTGGCGGAGGCACTGGATCCTCAG Sall His Fwd2 GGAGGCACTGGATCCTCAGATTTGGCAAACGATTC
Rev CCCGCTCGAG-CGTATCATATTTCACGTGC XhoI
Fu (orf46)-287-His Fwd CGGGGATCCGGGGGCGGCGGTGGCG BamHI
Rev CCCAAGCTTATCCTGCTCTTTTTTGCCGGC HindIII
Fu (orf46)-919-His Fwd CGCGGATCCGGTGGTGGTGGTCAAAGCAAGAGCATCCA BamHI
AACC
Rev CCCAAGCTTCGGGCGGTATTCGGGCTTC HindIII
Fu (orf46-919)- Fwd CCCCAAGCTTGGGGGCGGCGGTGGCG Hindlll 287-His Rev CCCGCTCGAGATCCTGCTCTTTTTTGCCGGC XhoI
Fu (orf46-287)- Fwd CCCAAGCTTGGTGGTGGTGGTGGTCAAAGCAAGAGCAT Hindlll 919-His CCAAACC
Rev CCCGCTCGAGCGGGCGGTATTCGGGCTT XhoI
(AG741)-961c-His Fwdl GGAGGCACTGGATCCGCAGCCACAAACGACGACGA XhoI
Fwd2 GCGGCCTCGAG-GGTGGCGGAGGCACTGGATCCGCAG
Rev CCCGCTCGAG-ACCCAGCTTGTAAGGTTG XhoI
(AG741)-961-His Fwdl GGAGGCACTGGATCCGCAGCCACAAACGACGACGA XhoI
Fwd2 GCGGCCTCGAG-GGTGGCGGAGGCACTGGATCCGCAG
Rev CCCGCTCGAG-CCACTCGTAATTGACGCC XhoI
(AG741)-983-His Fwd GCGGCCTCGAG- Xhol GGATCCGGCGGAGGCGGCACTTCTGCG
Rev CCCGCTCGAG-GAACCGGTAGCCTACG XhoI
(AG741 )-orf46.1- Fwdl GGAGGCACTGGATCCTCAGATTTGGCAAACGATTC Sail His Fwd2 GCGGCGTCGACGGTGGCGGAGGCACTGGATCCTCAGA
Rev CCCGCTCGAG-CGTATCATATTTCACGTGC Xhol (AG983)- Fwd GCGGCCTCGAG-GGATCCGGAGGGGGTGGTGTCGCC Xhol 741(MC58) -His Rev CCCGCTCGAG-TTGCTTGGCGGCAAG XhoI
(AG983)-961c-His Fwdl GGAGGCACTGGATCCGCAGCCACAAACGACGACGA XhoI
Fwd2 GCGGCCTCGAG-GGTGGCGGAGGCACTGGATCCGCAG
Rev CCCGCTCGAG-ACCCAGCTTGTAAGGTTG XhoI
(AG983)-961-His Fwdl GGAGGCACTGGATCCGCAGCCACAAACGACGACGA XhoI
Fwd2 GCGGCCTCGAG-GGTGGCGGAGGCACTGGATCCGCAG
Rev CCCGCTCGAG-CCACTCGTAATTGACGCC XhoI
(AG983)- Orf46.1- Fwdl GGAGGCACTGGATCCTCAGATTTGGCAAACGATTC SaII
His Fwd2 GCGGCGTCGACGGTGGCGGAGGCACTGGATCCTCAGA
Rev CCCGCTCGAG-CGTATCATATTTCACGTGC XhoI
* This primer was used as a Reverse primer for all the C terminal fusions of 287 to the His-tag.
Forward primers used in combination with the 287-His Reverse primer.
NB - All PCR reactions use strain 2996 unless otherwise specified (e.g. strain MC58) In all constructs starting with an ATG not followed by a unique Nhel site, the ATG codon is part of the NdeI site used for cloning. The constructs made using Nhel as a cloning site at the 5' end (e.g. all those containing 287 at the N-terminus) have two additional codons (GCT
AGC) fused to the coding sequence of the antigen.

Preparation of chromosomal DNA templates N.meningitidis strains 2996, MC58, 394.98, 1000 and BZ232 (and others) were grown to exponential phase in 100ml of GC medium, harvested by centrifugation, and resuspended in 5ml buffer (20% w/v sucrose, 50mM Tris-HC1, 50mM EDTA, pH8). After 10 minutes incubation on ice, the bacteria were lysed by adding lOml of lysis solution (50mM NaCl, 1%
Na-Sarkosyl, 50 g/ml Proteinase K), and the suspension incubated at 37 C for 2 hours. Two phenol extractions (equilibrated to pH 8) and one CHC13/isoamylalcohol (24:1) extraction were performed. DNA was precipitated by addition of 0.3M sodium acetate and 2 volumes of ethanol, and collected by centrifugation. The pellet was washed once with 70%(v/v) ethanol and redissolved in 4.Oml TE buffer (10mM Tris-HC1, 1mM EDTA, pH 8.0).
The DNA concentration was measured by reading OD260.
PCR Amplification The standard PCR protocol was as follows: 200ng of genomic DNA from 2996, MC581000, or BZ232 strains or lOng of plasmid DNA preparation of recombinant clones were used as template in the presence of 40 M of each oligonucletide primer, 400-800 M
dNTPs solution, 1x PCR buffer (including 1.5mM MgC12), 2.5 units TaqI DNA polymerase (using Perkin-Elmer AmpliTaQ, Boerhingher Mannheim ExpandTM Long Template).

After a preliminary 3 minute incubation of the whole mix at 95 C, each sample underwent a two-step amplification: the first 5 cycles were performed using the hybridisation temperature that excluded the restriction enzyme tail of the primer (Tml). This was followed by 30 cycles according to the hybridisation temperature calculated for the whole length oligos (Tm2).
Elongation times, performed at 68 C or 72 C, varied according to the length of the Orf to be amplified. In the case of Orfl the elongation time, starting from 3 minutes, was increased by 15 seconds each cycle. The cycles were completed with a 10 minute extension step at 72 C.
The amplified DNA was either loaded directly on a 1% agarose gel. The DNA
fragment corresponding to the band of correct size was purified from the gel using the Qiagen Gel Extraction Kit, following the manufacturer's protocol.

Digestion of PCR fragments and of the cloning vectors The purified DNA corresponding to the amplified fragment was digested with the appropriate restriction enzymes for cloning into pET-21b+, pET22b+ or pET-24b+. Digested fragments were purified using the QlAquick PCR purification kit (following the manufacturer's instructions) and eluted with either H2O or 10mM Tris, pH 8.5.
Plasmid vectors were digested with the appropriate restriction enzymes, loaded onto a 1.0% agarose gel and the band corresponding to the digested vector purified using the Qiagen QlAquick Gel Extraction Kit.
Cloning The fragments corresponding to each gene, previously digested and purified, were ligated into pET21b+, pET22b+ or pET-24b+. A molar ratio of 3:1 fragment/vector was used with T4 DNA ligase in the ligation buffer supplied by the manufacturer.

Recombinant plasmid was transformed into competent Ecoli DH5 or HB101 by incubating the ligase reaction solution and bacteria for 40 minutes on ice, then at 37 C
for 3 minutes.
This was followed by the addition of 800 1 LB broth and incubation at 37 C for 20 minutes.
The cells were centrifuged at maximum speed in an Eppendorf microfuge, resuspended in approximately 200pl of the supernatant and plated onto LB ampicillin (100mg/ml) agar.

Screening for recombinant clones was performed by growing randomly selected colonies overnight at 37 C in 4.Oml of LB broth + 100 g/ml ampicillin. Cells were pelleted and plasmid DNA extracted using the Qiagen QlAprep Spin Miniprep Kit, following the manufacturer's instructions. Approximately 1 g of each individual miniprep was digested with the appropriate restriction enzymes and the digest loaded onto a 1-1.5%
agarose gel (depending on the expected insert size), in parallel with the molecular weight marker (1kb DNA Ladder, GIBCO). Positive clones were selected on the basis of the size of insert.
Expression After cloning each gene into the expression vector, recombinant plasmids were transformed into E.coli strains suitable for expression of the recombinant protein. 1 l of each construct was used to transform E.coli BL21-DE3 as described above. Single recombinant colonies were inoculated into 2ml LB+Amp (100 g/ml), incubated at 37 C overnight, then diluted 1:30 in 20ml of LB+Amp (100 g/ml) in 100ml flasks, to give an OD600 between 0.1 and 0.2.
The flasks were incubated at 30 C or at 37 C in a gyratory water bath shaker until OD600 indicated exponential growth suitable for induction of expression (0.4-0.8 OD). Protein expression was induced by addition of 1.OmM IPTG. After 3 hours incubation at 30 C or 37 C the OD600 was measured and expression examined. 1.Oml of each sample was centrifuged in a microfuge, the pellet resuspended in PBS and analysed by SDS-PAGE and Coomassie Blue staining.

Purification of His-tagged proteins Various forms of 287 were cloned from strains 2996 and MC58. They were constructed with a C-terminus His-tagged fusion and included a mature form (aa 18-427), constructs with deletions (Al, A 2, A3 and A4) and clones composed of either B or C domains.
For each clone purified as a His-fusion, a single colony was streaked and grown overnight at 37 C on a LB/Amp (100 pg/ml) agar plate. An isolated colony from this plate was inoculated into 20m1 of LB/Amp (100 pg/ml) liquid medium and grown overnight, at 37 C with shaking.
The overnight culture was diluted 1:30 into 1.0 L LB/Amp (100 g/ml) liquid medium and allowed to grow at the optimal temperature (30 or 37 C) until the ODsso reached 0.6-0.8.
Expression of recombinant protein was induced by addition of IPTO (final concentration 1.0mM) and the culture incubated for a further 3 hours. Bacteria were harvested by centrifugation at 8000g for 15 min at 4 C. The bacterial pellet was resuspended in 7.5 ml of either (i) cold buffer A (300 mM NaCl, 50 mM phosphate buffer, 10 mM
imidazole, pH 8.0) for soluble proteins or (ii) buffer B (10mM Tris-HCI, 100 mM phosphate buffer, pH 8.8 and, optionally, 8M urea) for insoluble proteins. Proteins purified in a soluble form included 287 His, Al, A2, A3 and A4287-His, A4287MC58-lies, 287c-His and 287cMC58-His.
Protein 287bMC58-His was insoluble and purified accordingly. Cells were disrupted by sonication on ice four times for 30 sec at 40W using a Branson sonifier 450 and centrifuged at 13000xg for 30 min at 4 C. For insoluble proteins, pellets were resuspended in 2.0 ml buffer C (6 M guanidine hydrochloride, 100 mM phosphate buffer, 10 mM Tris-HCI, pH 7.5 and treated with 10 passes of a Dounce homogenizer. The homogenate was centrifuged at 13000g for 30 min and the supernatant retained Supernatants for both soluble and insoluble preparations were mixed with l50pl Nit resin (previously equilibrated with either buffer A
or buffer B, as appropriate) and incubated at room temperature with gentle agitation for 30 *
min. The resin was Chelating Sepharose Fast Flow (Pharmacia), prepared according to the manufacturer's protocol. The batch-wise preparation was centrifuged at 700g for 5 min-at 4 C and the supernatant discarded. The resin was washed twice (batch-wise) with 10ml buffer A or B for 10 min, resuspended in 1.0 ml buffer A or B and loaded onto a disposable column. The resin continued to be washed with either (i) buffer A at 4 C or (ii) buffer B at room temperature, until the OD o of the flow-through reached 0.02-0.01. The resin was further washed with either (i) cold buffer C (300mM NaCl, 50mM phosphate buffer, 20mM
imidazole, pH 8.0) or (ii) buffer 1) (10mM Tris-HCI, 100mM phosphate buffer, pH 6.3 and, optionally, 8M urea) until OD no of the flow-through reached 0.02-0.01. The His-fusion protein was eluted by addition of 700p1 of either (i) cold elution buffer A
(300 mM NaCI, 50mM phosphate buffer, 250 mM imidazole, pH 8.0) or (ii) elution buffer B (10 mM
Tris HCl, 100 mM phosphate buffer, pH 4.5 and, optionally, 8M urea) and fractions *Trade-mark collected until the 0028o, indicated all the recombinant protein was obtained.
20pl aliquots of each elution fraction were analysed by SDS-PAGE. Protein concentrations were estimated using the Bradford assay.

Renaturation of denatured His fusion proteins.

Denaturation was required to solubilize 287bMC8, so a renaturation step was employed prior to immunisation. Glycerol was added to the denatured fractions obtained above to give a final concentration of 10% v/v. The proteins were diluted to 200 pg/ml using dialysis buffer I (10% v/v glycerol, 0.5M.arginine, 50 mM phosphate, buff er, 5.0 mM reduced glutathione, 0.5 mM oxidised glutathione, 2.OM urea, pH 8.8) and dialysed against the same buffer for 12-14 hours at 4 C. Further dialysis was performed with buffer 11(10% v/v glycerol, 0.5M
arginine, 50mM phosphate buffer, 5.0mM reduced glutathione, 0.5mM oxidised glutathione, pH 8.8) for 12-14 hours at 4 C. Protein concentration was estimated using the formula:
Protein (mglml) = (1.55 x ODD) - (0.76 x ODgo) Immunization Balb/C mice were immunized with antigens on days 0, 21 and 35 and sera analyzed at day 49.
Sera analysis - ELISA
The acapsulated MenB M7 and the capsulated strains were plated on chocolate agar plates and incubated overnight at 37 C with 5% CO2. Bacterial colonies were collected from the agar plates using a sterile dracon swab and inoculated into Mueller-Hinton Broth (Difco) containing 0.25% glucose. Bacterial growth was monitored every 30 minutes by'following ODD. The bacteria were let to grow until the OD reached the value of 0.4-0.5.
The culture was centrifuged for 10 minutes at 4000rpm. The supernatant was discarded and bacteria were washed twice with PBS, resuspended in PBS containing 0.025% formaldehyde, and incubated for 1 hour at 37 C and then overnight at 4 C with stirring. 100pl bacterial cells were added to each well of a 96 well Greiner plate and incubated overnight at 4 C. The wells were then washed three times with PBT washing buffer (0.1% Tween-20 in PBS).
200p1 of saturation buffer (2.7% polyvinylpyrrolidone 10 in water) was added to each well and the plates incubated for 2 hours at 37 C. Wells were washed three times with PBT.
200pl of diluted sera (Dilution buffer: 1% BSA, 0.1% Tween-20, 0.1% NaN3 in PBS) were added to each well and the plates incubated for 2 hours at 37 C. Wells were washed three times with PBT. 100pl of HRP-conjugated rabbit anti-mouse (Dako) serum diluted 1:2000 in dilution buffer were added to each well and the plates were incubated for 90 minutes at 37 C. Walls *Trade-mark were washed three times with PBT buffer. l00 1 of substrate buffer for HRP
(25m1 of citrate buffer pH5, 10mg of 0-phenildiamine and l0 1 of H202) were added to each well and the plates were left at room temperature for 20 minutes. 100 I 12.5% H2SO4 was added to each well and OD490 was followed. The ELISA titers were calculated abitrarely as the dilution of sera which gave an OD490 value of 0.4 above the level of preimmune sera. The ELISA was considered positive when the dilution of sera with OD490 of 0.4 was higher than 1:400.

Sera analysis - FAGS Scan bacteria binding assay The acapsulated MenB M7 strain was plated on chocolate agar plates and incubated overnight at 37 C with 5% CO2. Bacterial colonies were collected from the agar plates using a sterile dracon swab and inoculated into 4 tubes containing 8m1 each Mueller-Hinton Broth (Difco) containing 0.25% glucose. Bacterial growth was monitored every 30 minutes by following OD620. The bacteria were let to grow until the OD reached the value of 0.35-0.5.
The culture was centrifuged for 10 minutes at 4000rpm. The supernatant was discarded and the pellet was resuspended in blocking buffer (1% BSA in PBS, 0.4% NaN3) and centrifuged for 5 minutes at 4000rpm. Cells were resuspended in blocking buffer to reach OD620 of 0.05.
l00pl bacterial cells were added to each well of a Costar 96 well plate. 100 l of diluted (1:100, 1:200, 1:400) sera (in blocking buffer) were added to each well and plates incubated for 2 hours at 4 C. Cells were centrifuged for 5 minutes at 4000rpm, the supernatant aspirated and cells washed by addition of 200 1/well of blocking buffer in each well. 100 l of R-Phicoerytrin conjugated F(ab)2 goat anti-mouse, diluted 1:100, was added to each well and plates incubated for 1 hour at 4 C. Cells were spun down by centrifugation at 4000rpm for 5 minutes and washed by addition of 200 1/well of blocking buffer. The supernatant was aspirated and cells resuspended in 200pl/well of PBS, 0.25% formaldehyde.
Samples were transferred to FACScan tubes and read. The condition for FACScan (Laser Power 15mW) setting were: FL2 on; FSC-H threshold:92; FSC PMT Voltage: E 01; SSC PMT: 474;
Amp.
Gains 6.1; FL-2 PMT: 586; compensation values: 0.

Sera analysis - bactericidal assay N. nieningitidis strain 2996 was grown overnight at 37 C on chocolate agar plates (starting from a frozen stock) with 5% CO2. Colonies were collected and used to inoculate 7m1 Mueller-Hinton broth, containing 0.25% glucose to reach an OD620 of 0.05-0.08.
The culture was incubated for approximately 1.5 hours at 37 degrees with shacking until the OD620 reached the value of 0.23-0.24. Bacteria were diluted in 50mM Phosphate buffer pH 7.2 containing 10mM MgC12, lOmM CaC12 and 0.5% (w/v) BSA (assay buffer) at the working dilution of 105 CFU/ml. The total volume of the final reaction mixture was 50 pl with 25 pl of serial two fold dilution of test serum, 12.5 l of bacteria at the working dilution, 12.5 l of baby rabbit complement (final concentration 25% ).

Controls included bacteria incubated with complement serum, immune sera incubated with bacteria and with complement inactivated by heating at 56 C for 30'.
Immediately after the addition of the baby rabbit complement, l0 1 of the controls were plated on Mueller-Hinton agar plates using the tilt method (time 0). The 96-wells plate was incubated for 1 hour at 37 C with rotation. 7 l of each sample were plated on Mueller-Hinton agar plates as spots, whereas 1O 1 of the controls were plated on Mueller-Hinton agar plates using the tilt method (time 1). Agar plates were incubated for 18 hours at 37 degrees and the colonies corresponding to time 0 and time 1 were counted.

Sera analysis - western blots Purified proteins (500ng/lane), outer membrane vesicles (5 g) and total cell extracts (25 g) derived from MenB strain 2996 were loaded onto a 12% SDS-polyacrylamide gel and transferred to a nitrocellulose membrane. The transfer was performed for 2 hours at 150mA
at 4 C, using transfer buffer (0.3% Tris base, 1.44% glycine, 20% (v/v) methanol). The membrane was saturated by overnight incubation at 4 C in saturation buffer (10% skimmed milk, 0.1% Triton X100 in PBS). The membrane was washed twice with washing buffer (3%
skimmed milk, 0.1% Triton X100 in PBS) and incubated for 2 hours at 37 C with mice sera diluted 1:200 in washing buffer. The membrane was washed twice and incubated for 90 minutes with a 1:2000 dilution of horseradish peroxidase labelled anti-mouse 1g. The membrane was washed twice with 0.1% Triton X100 in PBS and developed with the Opti-4CN Substrate Kit (Bio-Rad). The reaction was stopped by adding water.

The OMVs were prepared as follows: N. meningitidis strain 2996 was grown overnight at 37 degrees with 5% CO2 on 5 GC plates, harvested with a loop and resuspended in 10 ml of 20mM Tris-HC1 pH 7.5, 2 mM EDTA. Heat inactivation was performed at 56 C for minutes and the bacteria disrupted by sonication for 5 minutes on ice (50%
duty cycle, 50%
output, Branson sonifier 3 mm microtip). Unbroken cells were removed by centrifugation at 5000g for 10 minutes, the supernatant containing the total cell envelope fraction recovered and further centrifuged overnight at 50000g at the temperature of 4 C . The pellet containing the membranes was resuspended in 2% sarkosyl, 20mM Tris-HCl pH 7.5, 2 mM EDTA
and incubated at room temperature for 20 minutes to solubilise the inner membranes. The suspension was centrifuged at 10000g for 10 minutes to remove aggregates, the supernatant was further `centrifuged at 50000g for 3 hours. The pellet, containing the outer membranes *Trade-mark was washed in PBS and resuspended in the same buffer. Protein concentration was measured by the D.C. Bio-Rad Protein assay (Modified Lowry method), using BSA as a standard.
Total cell extracts were prepared as follows: N. meningitidis strain 2996 was grown overnight on a GC plate, harvested with a loop and resuspended in lml of 20mM
Tris-HC1.
Heat inactivation was performed at 56 C for 30 minutes.
SEQUENCE LISTING
<110> Chiron SpA

<120> Hybrid Expression of Neisserial Proteins <130> PAT 52959W-1 <140> 2,400,562 <141> 2001-02-28 <150> GB 0004695.3 <151> 2000-02-28 <150> GB 0027675.8 <151> 2000-11-13 <160> 121 <170> SeqWin99, version 1.02 <210> 1 <211> 608 <212> PRT
<213> Neisseria meningitidis <400> 1 Leu Gly Ile Ser Arg Lys Ile Ser Leu Ile Leu Ser Ile Leu Ala Val Cys Leu Pro Met His Ala His Ala Ser Asp Leu Ala Asn Asp Ser Phe Ile Arg Gln Val Leu Asp Arg Gln His Phe Glu Pro Asp Gly Lys Tyr His Leu Phe Gly Ser Arg Gly Glu Leu Ala Glu Arg Ser Gly His Ile Gly Leu Gly Lys Ile Gln Ser His Gln Leu Gly Asn Leu Met Ile Gln Gln Ala Ala Ile Lys Gly Asn Ile Gly Tyr Ile Val Arg Phe Ser Asp His Gly His Glu Val His Ser Pro Phe Asp Asn His Ala Ser His Ser Asp Ser Asp Glu Ala Gly Ser Pro Val Asp Gly Phe Ser Leu Tyr Arg Ile His Trp Asp Gly Tyr Glu His His Pro Ala Asp Gly Tyr Asp Gly Pro Gin Gly Gly Gly Tyr Pro Ala Pro Lys Gly Ala Arg Asp Ile Tyr Ser Tyr Asp Ile Lys Gly Val Ala Gln Asn Ile Arg Leu Asn Leu Thr Asp Asn Arg Ser Thr Gly Gln Arg Leu Ala Asp Arg Phe His Asn Ala Gly Ser Met Leu Thr Gln Gly Val Gly Asp Gly Phe Lys Arg Ala Thr Arg Tyr Ser Pro Glu Leu Asp Arg Ser Gly Asn Ala Ala Glu Ala Phe Asn Gly Thr Ala Asp Ile Val Lys Asn Ile Ile Gly Ala Ala Gly Glu Ile Val Gly Ala Gly Asp Ala Val Gln Gly Ile Ser Glu Gly Ser Asn Ile Ala Val Met His Gly Leu Gly Leu Leu Ser Thr Glu Asn Lys Met Ala Arg Ile Asn Asp Leu Ala Asp Met Ala Gln Leu Lys Asp Tyr Ala Ala Ala Ala Ile Arg Asp Trp Ala Val Gln Asn Pro Asn Ala Ala Gln Gly Ile Glu Ala Val Ser Asn Ile Phe Met Ala Ala Ile Pro Ile Lys Gly Ile Gly Ala Val Arg Gly Lys Tyr Gly Leu Gly Gly Ile Thr Ala His Pro Ile Lys Arg Ser Gln Met Gly Ala Ile Ala Leu Pro Lys Gly Lys Ser Ala Val Ser Asp Asn Phe Ala Asp Ala Ala Tyr Ala Lys Tyr Pro Ser Pro Tyr His Ser Arg Asn Ile Arg Ser Asn Leu Glu Gln Arg Tyr Gly Lys Glu Asn Ile Thr Ser Ser Thr Val Pro Pro Ser Asn Gly Lys Asn Val Lys Leu Ala Asp Gln Arg His Pro Lys Thr Gly Val Pro Phe Asp Gly Lys Gly Phe Pro Asn Phe Glu Lys His Val Lys Tyr Asp Thr Lys Leu Asp Ile Gln Glu Leu Ser Gly Gly Gly Ile Pro Lys Ala Lys Pro Val Ser Asp Ala Lys Pro Arg Trp Glu Val Asp Arg Lys Leu Asn Lys Leu Thr Thr Arg Glu Gln Val Glu Lys Asn Val Gln Glu Ile Arg Asn Gly Asn Lys Asn Ser Asn Phe Ser Gln His Ala Gln Leu Glu Arg Glu Ile Asn Lys Leu Lys Ser Ala Asp Glu Ile Asn Phe Ala Asp Gly Met Gly Lys Phe Thr Asp Ser Met Asn Asp Lys Ala Phe Ser Arg Leu Val Lys Ser Val Lys Glu Asn Gly Phe Thr Asn Pro Val Val Glu Tyr Val Glu Ile Asn Gly Lys Ala Tyr Ile Val Arg Gly Asn Asn Arg Val Phe Ala Ala Glu Tyr Leu Gly Arg Ile His Glu Leu Lys Phe Lys Lys Val Asp Phe Pro Val Pro Asn Thr Ser Trp Lys Asn Pro Thr Asp Val Leu Asn Glu Ser Gly Asn Val Lys Arg Pro Arg Tyr Arg Ser Lys <210> 2 <211> 464 <212> PRT
<213> Artificial Sequence <220>
<223> deltaG287 <400> 2 Ser Pro Asp Val Lys Ser Ala Asp Thr Leu Ser Lys Pro Ala Ala Pro Val Val Ser Glu Lys Glu Thr Glu Ala Lys Glu Asp Ala Pro Gln Ala Gly Ser Gln Gly Gln Gly Ala Pro Ser Ala Gln Gly Ser Gln Asp Met Ala Ala Val Ser Glu Glu Asn Thr Gly Asn Gly Gly Ala Val Thr Ala Asp Asn Pro Lys Asn Glu Asp Glu Val Ala Gln Asn Asp Met Pro Gin Asn Ala Ala Gly Thr Asp Ser Ser Thr Pro Asn His Thr Pro Asp Pro Asn Met Leu Ala Gly Asn Met Glu Asn Gln Ala Thr Asp Ala Gly Glu Ser Ser Gln Pro Ala Asn Gln Pro Asp Met Ala Asn Ala Ala Asp Gly Met Gln Gly Asp Asp Pro Ser Ala Gly Gly Gln Asn Ala Gly Asn Thr Ala Ala Gln Gly Ala Asn Gln Ala Gly Asn Asn Gln Ala Ala Gly Ser Ser Asp Pro Ile Pro Ala Ser Asn Pro Ala Pro Ala Asn Gly Gly Ser Asn Phe Gly Arg Val Asp Leu Ala Asn Gly Val Leu Ile Asp Gly Pro Ser Gln Asn Ile Thr Leu Thr His Cys Lys Gly Asp Ser Cys Ser Gly Asn Asn Phe Leu Asp Glu Glu Val Gln Leu Lys Ser Glu Phe Glu Lys Leu Ser Asp Ala Asp Lys Ile Ser Asn Tyr Lys Lys Asp Gly Lys Asn Asp Lys Phe Val Gly Leu Val Ala Asp Ser Val Gln Met Lys Gly Ile Asn Gln Tyr Ile Ile Phe Tyr Lys Pro Lys Pro Thr Ser Phe Ala Arg Phe Arg Arg Ser Ala Arg Ser Arg Arg Ser Leu Pro Ala Glu Met Pro Leu Ile Pro Val Asn Gln Ala Asp Thr Leu Ile Val Asp Gly Glu Ala Val Ser Leu Thr Gly His Ser Gly Asn Ile Phe Ala Pro Glu Gly Asn Tyr Arg Tyr Leu Thr Tyr Gly Ala Glu Lys Leu Pro Gly Gly Ser Tyr Ala Leu Arg Val Gln Gly Glu Pro Ala Lys Gly Glu Met Leu Ala Gly Ala Ala Val Tyr Asn Gly Glu Val Leu His Phe His Thr Glu Asn Gly Arg Pro Tyr Pro Thr Arg Gly Arg Phe Ala Ala Lys Val Asp Phe Gly Ser Lys Ser Val Asp Gly Ile Ile Asp Ser Gly Asp Asp Leu His Met Gly Thr Gln Lys Phe Lys Ala Ala Ile Asp Gly Asn Gly Phe Lys Gly Thr Trp Thr Glu Asn Gly Ser Gly Asp Val Ser Gly Lys Phe Tyr Gly Pro Ala Gly Glu Glu Val Ala Gly Lys Tyr Ser Tyr Arg Pro Thr Asp Ala Glu Lys Gly Gly Phe Gly Val Phe Ala Gly Lys Lys Glu Gln Asp <210> 3 <211> 2505 <212> DNA
<213> Artificial sequence <220>
<223> deltaG287-919 <400> 3 atggctagcc ccgatgttaa atcggcggac acgctgtcaa aaccggccgc tcctgttgtt 60 gctgaaaaag agacagaggt aaaagaagat gcgccacagg caggttctca aggacagggc 120 gcgccatcca cacaaggcag ccaagatatg gcggcagttt cggcagaaaa tacaggcaat 180 ggcggtgcgg caacaacgga caaacccaaa aatgaagacg agggaccgca aaatgatatg 240 ccgcaaaatt ccgccgaatc cgcaaatcaa acagggaaca accaacccgc cgattcttca 300 gattccgccc ccgcgtcaaa ccctgcacct gcgaatggcg gtagcaattt tggaagggtt 360 gatttggcta atggcgtttt gattgatggg ccgtcgcaaa atataacgtt gacccactgt 420 aaaggcgatt cttgtaatgg tgataattta ttggatgaag aagcaccgtc aaaatcagaa 480 tttgaaaatt taaatgagtc tgaacgaatt gagaaatata agaaagatgg gaaaagcgat 540 aaatttacta atttggttgc gacagcagtt caagctaatg gaactaacaa atatgtcatc 600 atttataaag acaagtccgc ttcatcttca tctgcgcgat tcaggcgttc tgcacggtcg 660 aggaggtcgc ttcctgccga gatgccgcta atccccgtca atcaggcgga tacgctgatt 720 gtcgatgggg aagcggtcag cctgacgggg cattccggca atatcttcgc gcccgaaggg 780 aattaccggt atctgactta cggggcggaa aaattgcccg gcggatcgta tgccctccgt 840 gtgcaaggcg aaccggcaaa aggcgaaatg cttgctggca cggccgtgta caacggcgaa 900 gtgctgcatt ttcatacgga aaacggccgt ccgtacccga ctaaaggcag gtttgccgca 960 aaagtcgatt tcggcagcaa atctgtggac ggcattatcg acagcggcga tgatttgcat 1020 atgggtacgc aaaaattcaa agccgccatc gatggaaacg gctttaaggg gacttggacg 1080 gaaaatggcg gcggggatgt ttccggaagg ttttacggcc cggccggcga ggaagtggcg 1140 ggaaaataca gctatcgccc gacagatgcg gaaaagggcg gattcggcgt gtttgccggc 1200 aaaaaagagc aggatggatc cggaggagga ggatgccaaa gcaagagcat ccaaaccttt 1260 ccgcaacccg acacatccgt catcaacggc ccggaccggc cggtcggcat ccccgacccc 1320 gccggaacga cggtcggcgg cggcggggcc gtctataccg ttgtaccgca cctgtccctg 1380 ccccactggg cggcgcagga tttcgccaaa agcctgcaat ccttccgcct cggctgcgcc 1440 aatttgaaaa accgccaagg ctggcaggat gtgtgcgccc aagcctttca aacccccgtc 1500 cattcctttc aggcaaaaca gttttttgaa cgctatttca cgccgtggca ggttgcaggc 1560 aacggaagcc ttgccggtac ggttaccggc tattacgagc cggtgctgaa gggcgacgac 1620 aggcggacgg cacaagcccg cttcccgatt tacggtattc ccgacgattt tatctccgtc 1680 cccctgcctg ccggtttgcg gagcggaaaa gcccttgtcc gcatcaggca gacgggaaaa 1740 aacagcggca caatcgacaa taccggcggc acacataccg ccgacctctc ccgattcccc 1800 atcaccgcgc gcacaacggc aatcaaaggc aggtttgaag gaagccgctt cctcccctac 1860 cacacgcgca accaaatcaa cggcggcgcg cttgacggca aagccccgat actcggttac 1920 gccgaagacc ccgtcgaact tttttttatg cacatccaag gctcgggccg tctgaaaacc 1980 ccgtccggca aatacatccg catcggctat gccgacaaaa acgaacatcc ctacgtttcc 2040 atcggacgct atatggcgga caaaggctac ctcaagctcg ggcagacctc gatgcagggc 2100 atcaaagcct atatgcggca aaatccgcaa cgcctcgCcg aagttttggg tcaaaacccc 2160 agctatatct ttttccgcga gcttgccgga agcagcaatg acggtcccgt cggcgcactg 2220 ggcacgccgt tgatggggga atatgccggc gcagtcgacc ggcactacat taccttgggc 2280 gcgcccttat ttgtcgccac cgcccatccg gttacccgca aagccctcaa ccgcctgatt 2340 atggcgcagg ataccggcag cgcgattaaa ggcgcggtgc gcgtggatta tttttgggga 2400 tacggcgacg aagccggcga acttgccggc aaacagaaaa ccacgggtta cgtctggcag 2460 ctcctaccca acggtatgaa gcccgaatac cgcccgtaac tcgag 2505 <210> 4 <211> 832 <212> PRT
<213> Artificial Sequence <220>
<223> deltaG287-919 <400> 4 Met Ala Ser Pro Asp Val Lys Ser Ala Asp Thr Leu Ser Lys Pro Ala Ala Pro Val Val Ala Glu Lys Glu Thr Glu Val Lys Glu Asp Ala Pro Gln Ala Gly Ser Gln Gly Gln Gly Ala Pro Ser Thr Gln Gly Ser Gln Asp Met Ala Ala Val Ser Ala Glu Asn Thr Gly Asn Gly Gly Ala Ala Thr Thr Asp Lys Pro Lys Asn Glu Asp Glu Gly Pro Gln Asn Asp Met Pro Gln Asn Ser Ala Glu Ser Ala Asn Gln Thr Gly Asn Asn Gln Pro Ala Asp Ser Ser Asp Ser Ala Pro Ala Ser Asn Pro Ala Pro Ala Asn Gly Gly Ser Asn Phe Gly Arg Val Asp Leu Ala Asn Gly Val Leu Ile Asp Gly Pro Ser Gln Asn Ile Thr Leu Thr His Cys Lys Gly Asp Ser Cys Asn Gly Asp Asn Leu Leu Asp Glu Glu Ala Pro Ser Lys Ser Glu Phe Glu Asn Leu Asn Glu Ser Glu Arg Ile Glu Lys Tyr Lys Lys Asp Gly Lys Ser Asp Lys Phe Thr Asn Leu Val Ala Thr Ala Val Gln Ala Asn Gly Thr Asn Lys Tyr Val Ile Ile Tyr Lys Asp Lys Ser Ala Ser Ser Ser Ser Ala Arg Phe Arg Arg Ser Ala Arg Ser Arg Arg Ser Leu Pro Ala Glu Met Pro Leu Ile Pro Val Asn Gln Ala Asp Thr Leu Ile Val Asp Gly Glu Ala Val Ser Leu Thr Gly His Ser Gly Asn Ile Phe Ala Pro Glu Gly Asn Tyr Arg Tyr Leu Thr Tyr Gly Ala Glu Lys Leu Pro Gly Gly Ser Tyr Ala Leu Arg Val Gln Gly Glu Pro Ala Lys Gly Glu Met Leu Ala Gly Thr Ala Val Tyr Asn Gly Glu Val Leu His Phe His Thr Glu Asn Gly Arg Pro Tyr Pro Thr Arg Gly Arg Phe Ala Ala Lys Val Asp Phe Gly Ser Lys Ser Val Asp Gly Ile Ile Asp Ser Gly Asp Asp Leu His Met Gly Thr Gln Lys Phe Lys Ala Ala Ile Asp Gly Asn Gly Phe Lys Gly Thr Trp Thr Glu Asn Gly Gly Gly Asp Val Ser Gly Arg Phe Tyr Gly Pro Ala Gly Glu Glu Val Ala Gly Lys Tyr Ser Tyr Arg Pro Thr Asp Ala Glu Lys Gly Gly Phe Gly Val Phe Ala Gly Lys Lys Glu Gln Asp Gly Ser Gly Gly Gly Gly Cys Gln Ser Lys Ser Ile Gln Thr Phe Pro Gln Pro Asp Thr Ser Val Ile Asn Gly Pro Asp Arg Pro Val Gly Ile Pro Asp Pro Ala Gly Thr Thr Val Gly Gly Gly Gly Ala Val Tyr Thr Val Val Pro His Leu Ser Leu Pro His Trp Ala Ala Gln Asp Phe Ala Lys Ser Leu Gln Ser Phe Arg Leu Gly Cys Ala Asn Leu Lys Asn Arg Gln Gly Trp Gln Asp Val Cys Ala Gln Ala Phe Gln Thr Pro Val His Ser Phe Gln Ala Lys Gln Phe Phe Glu Arg Tyr Phe Thr Pro Trp Gln Val Ala Gly Asn Gly Ser Leu Ala Gly Thr Val Thr Gly Tyr Tyr Glu Pro Val Leu Lys Gly Asp Asp Arg Arg Thr Ala Gln Ala Arg Phe Pro Ile Tyr Gly Ile Pro Asp Asp Phe Ile Ser Val Pro Leu Pro Ala Gly Leu Arg Ser Gly Lys Ala Leu Val Arg Ile Arg Gln Thr Gly Lys Asn Ser Gly Thr Ile Asp Asn Thr Gly Gly Thr His Thr Ala Asp Leu Ser Arg Phe Pro Ile Thr Ala Arg Thr Thr Ala Ile Lys Gly Arg Phe Glu Gly Ser Arg Phe Leu Pro Tyr His Thr Arg Asn Gln Ile Asn Gly Gly Ala Leu Asp Gly Lys Ala Pro Ile Leu Gly Tyr Ala Glu Asp Pro Val Glu Leu Phe Phe Met His Ile Gln Gly Ser Gly Arg Leu Lys Thr Pro Ser Gly Lys Tyr Ile Arg Ile Gly Tyr Ala Asp Lys Asn Glu His Pro Tyr Val Ser Ile Gly Arg Tyr Met Ala Asp Lys Gly Tyr Leu Lys Leu Gly Gln Thr Ser Met Gln Gly Ile Lys Ala Tyr Met Arg Gln Asn Pro Gln Arg Leu Ala Glu Val Leu Gly Gln Asn Pro Ser Tyr Ile Phe Phe Arg Glu Leu Ala Gly Ser Ser Asn Asp Gly Pro Val Gly Ala Leu Gly Thr Pro Leu Met Gly Glu Tyr Ala Gly Ala Val Asp Arg His Tyr Ile Thr Leu Gly Ala Pro Leu Phe Val Ala Thr Ala His Pro Val Thr Arg Lys Ala Leu Asn Arg Leu Ile Met Ala Gln Asp Thr Gly Ser Ala Ile Lys Gly Ala Val Arg Val Asp Tyr Phe Trp Gly Tyr Gly Asp Glu Ala Gly Glu Leu Ala Gly Lys Gln Lys Thr Thr Gly Tyr Val Trp Gln Leu Leu Pro Asn Gly Met Lys Pro Glu Tyr Arg Pro <210> 5 <211> 1746 <212> DNA
<213> Artificial Sequence <220>
<223> deltaG287-953 <400> 5 atggctagcc ccgatgttaa atcggcggac acgctgtcaa aaccggccgc tcctgttgtt 60 gctgaaaaag agacagaggt aaaagaagat gcgccacagg caggttctca aggacagggc 120 gcgccatcca cacaaggcag ccaagatatg gcggcagttt cggcagaaaa tacaggcaat 180 ggcggtgcgg caacaacgga caaacccaaa aatgaagacg agggaccgca aaatgatatg 240 ccgcaaaatt ccgccgaatc cgcaaatcaa acagggaaca accaacccgc cgattcttca 300 gattccgccc ccgcgtcaaa ccctgcacct gcgaatggcg gtagcaattt tggaagggtt 360 gatttggcta atggcgtttt gattgatggg ccgtcgcaaa atataacgtt gacccactgt 420 aaaggcgatt cttgtaatgg tgataattta ttggatgaag aagcaccgtc aaaatcagaa 480 tttgaaaatt taaatgagtc tgaacgaatt gagaaatata agaaagatgg gaaaagcgat 540 aaatttacta atttggttgc gacagcagtt caagctaatg gaactaacaa atatgtcatc 600 atttataaag acaagtccgc ttcatcttca tctgcgcgat tcaggcgttc tgcacggtcg 660 aggaggtcgc ttcctgccga gatgccgcta atccccgtca atcaggcgga tacgctgatt 720 gtcgatgggg aagcggtcag cctgacgggg cattccggca atatcttcgc gcccgaaggg 780 aattaccggt atctgactta cggggcggaa aaattgcccg gcggatcgta tgccctccgt 840 gtgcaaggcg aaccggcaaa aggcgaaatg cttgctggca cggccgtgta caacggcgaa 900 gtgctgcatt ttcatacgga aaacggccgt ccgtacccga ctagaggcag gtttgccgca 960 aaagtcgatt tcggcagcaa atctgtggac ggcattatcg acagcggcga tgatttgcat 1020 atgggtacgc aaaaattcaa agccgccatc gatggaaacg gctttaaggg gacttggacg 1080 gaaaatggcg gcggggatgt ttccggaagg ttttacggcc cggccggcga ggaagtggcg 1140 ggaaaataca gctatcgccc gacagatgcg gaaaagggcg gattcggcgt gtttgccggc 1200 aaaaaagagc aggatggatc cggaggagga ggagccacct acaaagtgga cgaatatcac 1260 gccaacgccc gtttcgccat cgaccatttc aacaccagca ccaacgtcgg cggtttttac 1320 ggtctgaccg gttccgtcga gttcgaccaa gcaaaacgcg acggtaaaat cgacatcacc 1380 atccccgttg ccaacctgca aagcggtttg caacacttta ccgaccacct gaaatcagcc 1440 gacatcttcg atgccgccca atatccggac atccgctttg tttccaccaa attcaacttc 1500 aacggcaaaa aactggtttc cgttgacggc aacctgacca tgcacggcaa aaccgccccc 1560 gtcaaactca aagccgaaaa attcaactgc taccaaagcc cgatggcgaa aaccgaagtt 1620 tgcggcggcg acttcagcac caccatcgac cgcaccaaat ggggcgtgga ctacctcgtt 1680 aacgttggta tgaccaaaag cgtccgcatc gacatccaaa tcgaggcagc caaacaataa 1740 ctcgag 1746 <210> 6 <211> 579 <212> PRT
<213> Artificial Sequence <220>
<223> deltaG287-953 <400> 6 Met Ala Ser Pro Asp Val Lys Ser Ala Asp Thr Leu Ser Lys Pro Ala Ala Pro Val Val Ala Glu Lys Glu Thr Glu Val Lys Glu Asp Ala Pro Gln Ala Gly Ser Gln Gly Gln Gly Ala Pro Ser Thr Gln Gly Ser Gln Asp Met Ala Ala Val Ser Ala Glu Asn Thr Gly Asn Gly Gly Ala Ala Thr Thr Asp Lys Pro Lys Asn Glu Asp Glu Gly Pro Gln Asn Asp Met Pro Gln Asn Ser Ala Glu Ser Ala Asn Gln Thr Gly Asn Asn Gln Pro Ala Asp Ser Ser Asp Ser Ala Pro Ala Ser Asn Pro Ala Pro Ala Asn Gly Gly Ser Asn Phe Gly Arg Val Asp Leu Ala Asn Gly Val Leu Ile Asp Gly Pro Ser Gln Asn Ile Thr Leu Thr His Cys Lys Gly Asp Ser Cys Asn Gly Asp Asn Leu Leu Asp Glu Glu Ala Pro Ser Lys Ser Glu Phe Glu Asn Leu Asn Glu Ser Glu Arg Ile Glu Lys Tyr Lys Lys Asp Gly Lys Ser Asp Lys Phe Thr Asn Leu Val Ala Thr Ala Val Gln Ala Asn Gly Thr Asn Lys Tyr Val Ile Ile Tyr Lys Asp Lys Ser Ala Ser Ser Ser Ser Ala Arg Phe Arg Arg Ser Ala Arg Ser Arg Arg Ser Leu Pro Ala Glu Met Pro Leu Ile Pro Val Asn Gln Ala Asp Thr Leu Ile Val Asp Gly Glu Ala Val Ser Leu Thr Gly His Ser Gly Asn Ile Phe Ala Pro Glu Gly Asn Tyr Arg Tyr Leu Thr Tyr Gly Ala Glu Lys Leu Pro Gly Gly Ser Tyr Ala Leu Arg Val Gln Gly Glu Pro Ala Lys Gly Glu Met Leu Ala Gly Thr Ala Val Tyr Asn Gly Glu Val Leu His Phe His Thr Glu Asn Gly Arg Pro Tyr Pro Thr Arg Gly Arg Phe Ala Ala Lys Val Asp Phe Gly Ser Lys Ser Val Asp Gly Ile Ile Asp Ser Gly Asp Asp Leu His Met Gly Thr Gln Lys Phe Lys Ala Ala Ile Asp Gly Asn Gly Phe Lys Gly Thr Trp Thr Glu Asn Gly Gly Gly Asp Val Ser Gly Arg Phe Tyr Gly Pro Ala Gly Glu Glu Val Ala Gly Lys Tyr Ser Tyr Arg Pro Thr Asp Ala Glu Lys Gly Gly Phe Gly Val Phe Ala Gly Lys Lys Glu Gin Asp Gly Ser Gly Gly Gly Gly Ala Thr Tyr Lys Val Asp Glu Tyr His Ala Asn Ala Arg Phe Ala Ile Asp His Phe Asn Thr Ser Thr Asn Val Gly Gly Phe Tyr Gly Leu Thr Gly Ser Val Glu Phe Asp Gln Ala Lys Arg Asp Gly Lys Ile Asp Ile Thr Ile Pro Val Ala Asn Leu Gln Ser Gly Ser Gln His Phe Thr Asp His Leu Lys Ser Ala Asp Ile Phe Asp Ala Ala Gln Tyr Pro Asp Ile Arg Phe Val Ser Thr Lys Phe Asn Phe Asn Gly Lys Lys Leu Val Ser Val Asp Gly Asn Leu Thr Met His Gly Lys Thr Ala Pro Val Lys Leu Lys Ala Glu Lys Phe Asn Cys Tyr Gln Ser Pro Met Ala Lys Thr Glu Val Cys Gly Gly Asp Phe Ser Thr Thr Ile Asp Arg Thr Lys Trp Gly Val Asp Tyr Leu Val Asn Val Gly Met Thr Lys Ser Val Arg Ile Asp Ile Gln Ile Glu Ala Ala Lys Gln <210> 7 <211> 2388 <212> DNA
<213> Artificial Sequence <220>
<223> deltaG287-961 <400> 7 atgggtaccc ccgatgttaa atcggcggac acgctgtcaa aaccggccgc tcctgttgtt 60 gctgaaaaag agacagaggt aaaagaagat gcgccacagg caggttctca aggacagggc 120 gcgccatcca cacaaggcag ccaagatatg gcggcagttt cggcagaaaa tacaggcaat 180 ggcggtgcgg caacaacgga caaacccaaa aatgaagacg agggaccgca aaatgatatg 240 ccgcaaaatt ccgccgaatc cgcaaatcaa acagggaaca accaacccgc cgattcttca 300 gattccgccc ccgcgtcaaa ccctgcacct gcgaatggcg gtagcaattt tggaagggtt 360 gatttggcta atggcgtttt gattgatggg ccgtcgcaaa atataacgtt gacccactgt 420 aaaggcgatt cttgtaatgg tgataattta ttggatgaag aagcaccgtc aaaatcagaa 480 tttgaaaatt taaatgagtc tgaacgaatt gagaaatata agaaagatgg gaaaagcgat 540 aaatttacta atttggttgc gacagcagtt caagctaatg gaactaacaa atatgtcatc 600 atttataaag acaagtccgc ttcatcttca tctgcgcgat tcaggcgttc tgcacggtcg 660 aggaggtcgc ttcctgccga gatgccgcta atccccgtca atcaggcgga tacgctgatt 720 gtcgatgggg aagcggtcag cctgacgggg cattccggca atatcttcgc gcccgaaggg 780 aattaccggt atctgactta cggggcggaa aaattgcccg gcggatcgta tgccctccgt 840 gtgcaaggcg aaccggcaaa aggcgaaatg cttgctggca cggccgtgta caacggcgaa 900 gtgctgcatt ttcatacgga aaacggccgt ccgtacccga ctagaggcag gtttgccgca 960 aaagtcgatt tcggcagcaa atctgtggac ggcattatcg acagcggcga tgatttgcat 1020 atgggtacgc aaaaattcaa agccgccatc gatggaaacg gctttaaggg gacttggacg 1080 gaaaatggcg gcggggatgt ttccggaagg ttttacggcc cggccggcga ggaagtggcg 1140 ggaaaataca gctatcgccc gacagatgcg gaaaagggcg gattcggcgt gtttgccggc 1200 aaaaaagagc aggatggatc cggaggagga ggagccacaa acgacgacga tgttaaaaaa 1260 gctgccactg tggccattgc tgctgcctac aacaatggcc aagaaatcaa cggtttcaaa 1320 gctggagaga ccatctacga cattgatgaa gacggcacaa ttaccaaaaa agacgcaact 1380 gcagccgatg ttgaagccga cgactttaaa ggtctgggtc tgaaaaaagt cgtgactaac 1440 ctgaccaaaa ccgtcaatga aaacaaacaa aacgtcgatg ccaaagtaaa agctgcagaa 1500 tctgaaatag aaaagttaac aaccaagtta gcagacactg atgccgcttt agcagatact 1560 gatgccgctc tggatgcaac caccaacgcc ttgaataaat tgggagaaaa tataacgaca 1620 tttgctgaag agactaagac aaatatcgta aaaattgatg aaaaattaga agccgtggct 1680 gataccgtcg acaagcatgc cgaagcattc aacgatatcg ccgattcatt ggatgaaacc 1740 aacactaagg cagacgaagc cgtcaaaacc gccaatgaag ccaaacagac ggccgaagaa 1800 accaaacaaa acgtcgatgc caaagtaaaa gctgcagaaa ctgcagcagg caaagccgaa 1860 gctgccgctg gcacagctaa tactgcagcc gacaaggccg aagctgtcgc tgcaaaagtt 1920 accgacatca aagctgatat cgctacgaac aaagataata ttgctaaaaa agcaaacagt 1980 gccgacgtgt acaccagaga agagtctgac agcaaatttg tcagaattga tggtctgaac 2040 gctactaccg aaaaattgga cacacgcttg gcttctgctg aaaaatccat tgccgatcac 2100 gatactcgcc tgaacggttt ggataaaaca gtgtcagacc tgcgcaaaga aacccgccaa 2160 ggccttgcag aacaagccgc gctctccggt ctgttccaac cttacaacgt gggtcggttc 2220 aatgtaacgg ctgcagtcgg cggctacaaa tccgaatcgg cagtcgccat cggtaccggc 2280 ttccgcttta ccgaaaactt tgccgccaaa gcaggcgtgg cagtcggcac ttcgtccggt 2340 tcttccgcag cctaccatgt cggcgtcaat tacgagtggt aactcgag 2388 <210> 8 <211> 793 <212> PRT
<213> Artificial Sequence <220>
<223> deltaG287-961 <400> 8 Met Ala Ser Pro Asp Val Lys Ser Ala Asp Thr Leu Ser Lys Pro Ala Ala Pro Val Val Ala Glu Lys Glu Thr Glu Val Lys Glu Asp Ala Pro Gln Ala Gly Ser Gln Gly Gln Gly Ala Pro Ser Thr Gln Gly Ser Gln Asp Met Ala Ala Val Ser Ala Glu Asn Thr Gly Asn Gly Gly Ala Ala Thr Thr Asp Lys Pro Lys Asn Glu Asp Glu Gly Pro Gln Asn Asp Met Pro Gln Asn Ser Ala Glu Ser Ala Asn Gln Thr Gly Asn Asn Gln Pro Ala Asp Ser Ser Asp Ser Ala Pro Ala Ser Asn Pro Ala Pro Ala Asn Gly Gly Ser Asn Phe Gly Arg Val Asp Leu Ala Asn Gly Val Leu Ile Asp Gly Pro Ser Gln Asn Ile Thr Leu Thr His Cys Lys Gly Asp Ser Cys Asn Gly Asp Asn Leu Leu Asp Glu Glu Ala Pro Ser Lys Ser Glu Phe Glu Asn Leu Asn Glu Ser Glu Arg Ile Glu Lys Tyr Lys Lys Asp Gly Lys Ser Asp Lys Phe Thr Asn Leu Val Ala Thr Ala Val Gln Ala Asn Gly Thr Asn Lys Tyr Val Ile Ile Tyr Lys Asp Lys Ser Ala Ser Ser Ser Ser Ala Arg Phe Arg Arg Ser Ala Arg Ser Arg Arg Ser Leu Pro Ala Glu Met Pro Leu Ile Pro Val Asn Gln Ala Asp Thr Leu Ile Val Asp Gly Glu Ala Val Ser Leu Thr Gly His Ser Gly Asn Ile Phe Ala Pro Glu Gly Asn Tyr Arg Tyr Leu Thr Tyr Gly Ala Glu Lys Leu Pro Gly Gly Ser Tyr Ala Leu Arg Val Gln Gly Glu Pro Ala Lys Gly Glu Met Leu Ala Gly Thr Ala Val Tyr Asn Gly Glu Val Leu His Phe His Thr Glu Asn Gly Arg Pro Tyr Pro Thr Arg Gly Arg Phe Ala Ala Lys Val Asp Phe Gly Ser Lys Ser Val Asp Gly Ile Ile Asp Ser Gly Asp Asp Leu His Met Gly Thr Gln Lys Phe Lys Ala Ala Ile Asp Gly Asn Gly Phe Lys Gly Thr Trp Thr Glu Asn Gly Gly Gly Asp Val Ser Gly Arg Phe Tyr Gly Pro Ala Gly Glu Glu Val Ala Gly Lys Tyr Ser Tyr Arg Pro Thr Asp Ala Glu Lys Gly Gly Phe Gly Val Phe Ala Gly Lys Lys Glu Gln Asp Gly Ser Gly Gly Gly Gly Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile Ala Ala Ala Tyr Asn Asn Gly Gln Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gln Thr Ala Glu Glu Thr Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gln Gly Leu Ala Glu Gln Ala Ala Leu Ser Gly Leu Phe Gln Pro Tyr Asn Val Gly Arg Phe Asn Val Thr Ala Ala Val Gly Gly Tyr Lys Ser Glu Ser Ala Val Ala Ile Gly Thr Gly Phe Arg Phe Thr Glu Asn Phe Ala Ala Lys Ala Gly Val Ala Val Gly Thr Ser Ser Gly Ser Ser Ala Ala Tyr His Val Gly Val Asn Tyr Glu Trp <210>1 9 <211> 2700 <212> DNA
<213> Artificial Sequence <220>
<223> deltaG287NZ-919 <400> 9 atggctagcc ccgatgtcaa gtcggcggac acgctgtcaa aacctgccgc ccctgttgtt 60 tctgaaaaag agacagaggc aaaggaagat gcgccacagg caggttctca aggacagggc 120 gcgccatccg cacaaggcgg tcaagatatg gcggcggttt cggaagaaaa tacaggcaat 180 ggcggtgcgg cagcaacgga caaacccaaa aatgaagacg agggggcgca aaatgatatg 240 ccgcaaaatg ccgccgatac agatagtttg acaccgaatc acaccccggc ttcgaatatg 300 ccggccggaa atatggaaaa ccaagcaccg gatgccgggg aatcggagca gccggcaaac 360 caaccggata tggcaaatac ggcggacgga atgcagggtg acgatccgtc ggcaggcggg 420 gaaaatgccg gcaatacggc tgcccaaggt acaaatcaag ccgaaaacaa tcaaaccgcc 480 ggttctcaaa atcctgcctc ttcaaccaat cctagcgcca cgaatagcgg tggtgatttt 540 ggaaggacga acgtgggcaa ttctgttgtg attgacgggc cgtcgcaaaa tataacgttg 600 acccactgta aaggcgattc ttgtagtggc aataatttct tggatgaaga agtacagcta 660 aaatcagaat ttgaaaaatt aagtgatgca gacaaaataa gtaattacaa gaaagatggg 720 aagaatgacg ggaagaatga taaatttgtc ggtttggttg ccgatagtgt gcagatgaag 780 ggaatcaatc aatatattat cttttataaa cctaaaccca cttcatttgc gcgatttagg 840 cgttctgcac ggtcgaggcg gtcgcttccg gccgagatgc cgctgattcc cgtcaatcag 900 gcggatacgc tgattgtcga tggggaagcg gtcagcctga cggcgcattc cggcaatatc 960 ttcgcgcccg aagggaatta ccggtatctg acttacgggg cggaaaaatt gcccggcgga 1020 tcgtatgccc tccgtgttca aggcgaacct tcaaaaggcg aaatgctcgc gggcacggca 1080 gtgtacaacg gcgaagtgct gcattttcat acggaaaacg gccgtccgtc cccgtccaga 1140 ggcaggtttg ccgcaaaagt cgatttcggc agcaaatctg tggacggcat tatcgacagc 1200 ggcgatggtt tgcatatggg tacgcaaaaa ttcaaagccg ccatcgatgg aaacggcttt 1260 aaggggactt ggacggaaaa tggcggcggg gatgtttccg gaaagtttta cggcccggcc 1320 ggcgaggaag tggcgggaaa atacagctat cgcccaacag atgcggaaaa gggcggattc 1380 ggcgtgtttg ccggcaaaaa agagcaggat ggatccggag gaggaggatg ccaaagcaag 1440 agcatccaaa cctttccgca acccgacaca tccgtcatca acggcccgga ccggccggtc 1500 ggcatccccg accccgccgg aacgacggtc ggcggcggcg gggccgtcta taccgttgta 1560 ccgcacctgt ccctgcccca ctgggcggcg caggatttcg ccaaaagcct gcaatccttc 1620 cgcctcggct gcgccaattt gaaaaaccgc caaggctggc aggatgtgtg cgcccaagcc 1680 tttcaaaccc ccgtccattc ctttcaggca aaacagtttt ttgaacgcta tttcacgccg 1740 tggcaggttg caggcaacgg aagccttgcc ggtacggtta ccggctatta cgagccggtg 1800 ctgaagggcg acgacaggcg gacggcacaa gcccgcttcc cgatttacgg tattcccgac 1860 gattttatct ccgtccccct gcctgccggt ttgcggagcg gaaaagccct tgtccgcatc 1920 aggcagacgg gaaaaaacag cggcacaatc gacaataccg gcggcacaca taccgccgac 1980 ctctcccgat tccccatcac cgcgcgcaca acggcaatca aaggcaggtt tgaaggaagc 2040 cgcttcctcc cctaccacac gcgcaaccaa atcaacggcg gcgcgcttga cggcaaagcc 2100 ccgatactcg gttacgccga agaccccgtc gaactttttt ttatgcacat ccaaggctcg 2160 ggccgtctga aaaccccgtc cggcaaatac atccgcatcg gctatgccga caaaaacgaa 2220 catccctacg tttccatcgg acgctatatg gcggacaaag gctacctcaa gctcgggcag 2280 acctcgatgc agggcatcaa agcctatatg cggcaaaatc cccaacgcct cgccgaagtt 2340 ttgggtcaaa accccagcta tatctttttc cgcgagcttg ccggaagcag caatgacggt 2400 cccgtcggcg cactgggcac gccgttgatg ggggaatatg ccggcgcagt cgaccggcac 2460 tacattacct tgggcgcgcc cttatttgtc gccaccgccc atccggttac ccgcaaagcc 2520 ctcaaccgcc tgattatggc gcaggatacc ggcagcgcga ttaaaggcgc ggtgcgcgtg 2580 gattattttt ggggatacgg cgacgaagcc ggcgaacttg ccggcaaaca gaaaaccacg 2640 ggttacgtct ggcagctcct acccaacggt atgaagcccg aataccgccc gtaaaagctt 2700 <210> 10 <211> 897 <212> PRT
<213> Artificial Sequence <220>
<223> deltaG287NZ-919 <400> 10 Met Ala Ser Pro Asp Val Lys Ser Ala Asp Thr Leu Ser Lys Pro Ala Ala Pro Val Val Ser Glu Lys Glu Thr Glu Ala Lys Glu Asp Ala Pro Gln Ala Gly Ser Gln Gly Gln Gly Ala Pro Ser Ala Gln Gly Gly Gln Asp Met Ala Ala Val Ser Glu Glu Asn Thr Gly Asn Gly Gly Ala Ala Ala Thr Asp Lys Pro Lys Asn Glu Asp Glu Gly Ala Gln Asn Asp Met Pro Gln Asn Ala Ala Asp Thr Asp Ser Leu Thr Pro Asn His Thr Pro Ala Ser Asn Met Pro Ala Gly Asn Met Glu Asn Gln Ala Pro Asp Ala Gly Glu Ser Glu Gln Pro Ala Asn Gln Pro Asp Met Ala Asn Thr Ala Asp Gly Met Gln Gly Asp Asp Pro Ser Ala Gly Gly Glu Asn Ala Gly Asn Thr Ala Ala Gln Gly Thr Asn Gln Ala Glu Asn Asn Gln Thr Ala Gly Ser Gln Asn Pro Ala Ser Ser Thr Asn Pro Ser Ala Thr Asn Ser Gly Gly Asp Phe Gly Arg Thr Asn Val Gly Asn Ser Val Val Ile Asp Gly Pro Ser Gln Asn Ile Thr Leu Thr His Cys Lys Gly Asp Ser Cys Ser Gly Asn Asn Phe Leu Asp Glu Glu Val Gln Leu Lys Ser Glu Phe Glu Lys Leu Ser Asp Ala Asp Lys Ile Ser Asn Tyr Lys Lys Asp Gly Lys Asn Asp Gly Lys Asn Asp Lys Phe Val Gly Leu Val Ala Asp Ser Val Gln Met Lys Gly Ile Asn Gln Tyr Ile Ile Phe Tyr Lys Pro Lys Pro Thr Ser Phe Ala Arg Phe Arg Arg Ser Ala Arg Ser Arg Arg Ser Leu Pro Ala Glu Met Pro Leu Ile Pro Val Asn Gln Ala Asp Thr Leu Ile Val Asp Gly Glu Ala Val Ser Leu Thr Gly His Ser Gly Asn Ile Phe Ala Pro Glu Gly Asn Tyr Arg Tyr Leu Thr Tyr Gly Ala Glu Lys Leu Pro Gly Gly Ser Tyr Ala Leu Arg Val Gln Gly Glu Pro Ser Lys Gly Glu Met Leu Ala Gly Thr Ala Val Tyr Asn Gly Glu Val Leu His Phe His Thr Glu Asn Gly Arg Pro Ser Pro Ser Arg Gly Arg Phe Ala Ala Lys Val Asp Phe Gly Ser Lys Ser Val Asp Gly Ile Ile Asp Ser Gly Asp Gly Leu His Met Gly Thr Gln Lys Phe Lys Ala Ala Ile Asp Gly Asn Gly Phe Lys Gly Thr Trp Thr Glu Asn Gly Gly Gly Asp Val Ser Gly Lys Phe Tyr Gly Pro Ala Gly Glu Glu Val Ala Gly Lys Tyr Ser Tyr Arg Pro Thr Asp Ala Glu Lys Gly Gly Phe Gly Val Phe Ala Gly Lys Lys Glu Gln Asp Gly Ser Gly Gly Gly Gly Cys Gln Ser Lys Ser Ile Gln Thr Phe Pro Gln Pro Asp Thr Ser Val Ile Asn Gly Pro Asp Arg Pro Val Gly Ile Pro Asp Pro Ala Gly Thr Thr Val Gly Gly Gly Gly Ala Val Tyr Thr Val Val Pro His Leu Ser Leu Pro His Trp Ala Ala Gln Asp Phe Ala Lys Ser Leu Gln Ser Phe Arg Leu Gly Cys Ala Asn Leu Lys Asn Arg Gln Gly Trp Gln Asp Val Cys Ala Gln Ala Phe Gln Thr Pro Val His Ser Phe Gln Ala Lys Gln Phe Phe Glu Arg Tyr Phe Thr Pro Trp Gln Val Ala Gly Asn Gly Ser Leu Ala Gly Thr Val Thr Gly Tyr Tyr Glu Pro Val Leu Lys Gly Asp Asp Arg Arg Thr Ala Gln Ala Arg Phe Pro Ile Tyr Gly Ile Pro Asp Asp Phe Ile Ser Val Pro Leu Pro Ala Gly Leu Arg Ser Gly Lys Ala Leu Val Arg Ile Arg Gln Thr Gly Lys Asn Ser Gly Thr Ile Asp Asn Thr Gly Gly Thr His Thr Ala Asp Leu Ser Arg Phe Pro Ile Thr Ala Arg Thr Thr Ala Ile Lys Gly Arg Phe Glu Gly Ser Arg Phe Leu Pro Tyr His Thr Arg Asn Gln Ile Asn Gly Gly Ala Leu Asp Gly Lys Ala Pro Ile Leu Gly Tyr Ala Glu Asp Pro Val Glu Leu Phe Phe Met His Ile Gln Gly Ser Gly Arg Leu Lys Thr Pro Ser Gly Lys Tyr Ile Arg Ile Gly Tyr Ala Asp Lys Asn Glu His Pro Tyr Val Ser Ile Gly Arg Tyr Met Ala Asp Lys Gly Tyr Leu Lys Leu Gly Gln Thr Ser Met Gln Gly Ile Lys Ala Tyr Met Arg Gln Asn Pro Gln Arg Leu Ala Glu Val Leu Gly Gln Asn Pro Ser Tyr Ile Phe Phe Arg Glu Leu Ala Gly Ser Ser Asn Asp Gly Pro Val Gly Ala Leu Gly Thr Pro Leu Met Gly Glu Tyr Ala Gly Ala Val Asp Arg His Tyr Ile Thr Leu Gly Ala Pro Leu Phe Val Ala Thr Ala His Pro Val Thr Arg Lys Ala Leu Asn Arg Leu Ile Met Ala Gln Asp Thr Gly Ser Ala Ile Lys Gly Ala Val Arg Val Asp Tyr Phe Trp Gly Tyr Gly Asp Glu Ala Gly Glu Leu Ala Gly Lys Gln Lys Thr Thr Gly Tyr Val Trp Gln Leu Leu Pro Asn Gly Met Lys Pro Glu Tyr Arg Pro <210> 11 <211> 1941 <212> DNA
<213> Artificial Sequence <220>
<223> deltaG287NZ-953 <400> 11 atggctagcc ccgatgtcaa gtcggcggac acgctgtcaa aacctgccgc ccctgttgtt 60 tctgaaaaag agacagaggc aaaggaagat gcgccacagg caggttctca aggacagggc 120 gcgccatccg cacaaggcgg tcaagatatg gcggcggttt cggaagaaaa tacaggcaat 180 ggcggtgcgg cagcaacgga caaacccaaa aatgaagacg agggggcgca aaatgatatg 240 ccgcaaaatg ccgccgatac agatagtttg acaccgaatc acaccccggc ttcgaatatg 300 ccggccggaa atatggaaaa ccaagcaccg gatgccgggg aatcggagca gccggcaaac 360 caaccggata tggcaaatac ggcggacgga atgcagggtg acgatccgtc ggcaggcggg 420 gaaaatgccg gcaatacggc tgcccaaggt acaaatcaag ccgaaaacaa tcaaaccgcc 480 ggttctcaaa atcctgcctc ttcaaccaat cctagcgcca cgaatagcgg tggtgatttt 540 ggaaggacga acgtgggcaa ttctgttgtg attgacgggc cgtcgcaaaa tataacgttg 600 acccactgta aaggcgattc ttgtagtggc aataatttct tggatgaaga agtacagcta 660 aaatcagaat ttgaaaaatt aagtgatgca gacaaaataa gtaattacaa gaaagatggg 720 aagaatgacg ggaagaatga taaatttgtc ggtttggttg ccgatagtgt gcagatgaag 780 ggaatcaatc aatatattat cttttataaa cctaaaccca cttcatttgc gcgatttagg 840 cgttctgcac ggtcgaggcg gtcgcttccg gccgagatgc cgctgattcc cgtcaatcag 900 gcggatacgc tgattgtcga tggggaagcg gtcagcctga cggggcattc cggcaatatc 960 ttcgcgcccg aagggaatta ccggtatctg acttacgggg cggaaaaatt gcccggcgga 1020 tcgtatgccc tccgtgttca aggcgaacct tcaaaaggcg aaatgctcgc gggcacggca 1080 gtgtacaacg gcgaagtgct gcattttcat acggaaaacg gccgtccgtc cccgtccaga 1140 ggcaggtttg ccgcaaaagt cgatttcggc agcaaatctg tggacggcat tatcgacagc 1200 ggcgatggtt tgcatatggg tacgcaaaaa ttcaaagccg ccatcgatgg aaacggcttt 1260 aaggggactt ggacggaaaa tggcggcggg gatgtttccg gaaagtttta cggcccggcc 1320 ggcgaggaag tgacgggaaa atacagctat cgcccaacag atgcggaaaa gggcggattc 1380 ggcgtgtttg ccggcaaaaa agagcaggat ggatccggag gaggaggagc cacctacaaa 1440 gtggacgaat atcacgccaa cgcccgtttc gccatcgacc atttcaacac cagcaccaac 1500 gtcggcggtt tttacggtct gaccggttcc gtcgagttcg accaagcaaa acgcgacggt 1560 aaaatcgaca tcaccatccc cgttgccaac ctgcaaagcg gttcgcaaca ctttaccgac 1620 cacctgaaat cagccgacat cttcgatgcc gcccaatatc cggacatccg ctttgtttcc 1680 accaaattca acttcaacgg caaaaaactg gtttccgttg acggcaacct gaccatgcac 1740 ggcaaaaccg cccccgtcaa actcaaagcc gaaaaattca actgctacca aagcccgatg 1800 gcgaaaaccg aagtttgcgg cggcgacttc agcaccacca tcgaccgcac caaatggggc 1860 gtggactacc tcgttaacgt tggtatgacc aaaagcgtcc gcatcgacat ccaaatcgag 1920 gcagccaaac aataaaagct t 1941 <210> 12 <211> 644 <212> PRT
<213> Artificial Sequence <220>
<223> deltaG287NZ-953 <400> 12 Met Ala Ser Pro Asp Val Lys Ser Ala Asp Thr Leu Ser Lys Pro Ala Ala Pro Val Val Ser Glu Lys Glu Thr Glu Ala Lys Glu Asp Ala Pro Gln Ala Gly Ser Gln Gly Gln Gly Ala Pro Ser Ala Gln Gly Gly Gln Asp Met Ala Ala Val Ser Glu Glu Asn Thr Gly Asn Gly Gly Ala Ala Ala Thr Asp Lys Pro Lys Asn Glu Asp Glu Gly Ala Gln Asn Asp Met Pro Gln Asn Ala Ala Asp Thr Asp Ser Leu Thr Pro Asn His Thr Pro Ala Ser Asn Met Pro Ala Gly Asn Met Glu Asn Gln Ala Pro Asp Ala Gly Glu Ser Glu Gln Pro Ala Asn Gln Pro Asp Met Ala Asn Thr Ala Asp Gly Met Gln Gly Asp Asp Pro Ser Ala Gly Gly Glu Asn Ala Gly Asn Thr Ala Ala Gln Gly Thr Asn Gln Ala Glu Asn Asn Gln Thr Ala Gly Ser Gln Asn Pro Ala Ser Ser Thr Asn Pro Ser Ala Thr Asn Ser Gly Gly Asp Phe Gly Arg Thr Asn Val Gly Asn Ser Val Val Ile Asp Gly Pro Ser Gln Asn Ile Thr Leu Thr His Cys Lys Gly Asp Ser Cys Ser Gly Asn Asn Phe Leu Asp Glu Glu Val Gln Leu Lys Ser Glu Phe Glu Lys Leu Ser Asp Ala Asp Lys Ile Ser Asn Tyr Lys Lys Asp Gly Lys Asn Asp Gly Lys Asn Asp Lys Phe Val Gly Leu Val Ala Asp Ser Val Gln Met Lys Gly Ile Asn Gln Tyr Ile Ile Phe Tyr Lys Pro Lys Pro Thr Ser Phe Ala Arg Phe Arg Arg Ser Ala Arg Ser Arg Arg Ser Leu Pro Ala Glu Met Pro Leu Ile Pro Val Asn Gln Ala Asp Thr Leu Ile Val Asp Gly Glu Ala Val Ser Leu Thr Gly His Ser Gly Asn Ile Phe Ala Pro Glu Gly Asn Tyr Arg Tyr Leu Thr Tyr Gly Ala Glu Lys Leu Pro Gly Gly Ser Tyr Ala Leu Arg Val Gln Gly Glu Pro Ser Lys Gly Glu Met Leu Ala Gly Thr Ala Val Tyr Asn Gly Glu Val Leu His Phe His Thr Glu Asn Gly Arg Pro Ser Pro Ser Arg Gly Arg Phe Ala Ala Lys Val Asp Phe Gly Ser Lys Ser Val Asp Gly Ile Ile Asp Ser Gly Asp Gly Leu His Met Gly Thr Gln Lys Phe Lys Ala Ala Ile Asp Gly Asn Gly Phe Lys Gly Thr Trp Thr Glu Asn Gly Gly Gly Asp Val Ser Gly Lys Phe Tyr Gly Pro Ala Gly Glu Glu Val Ala Gly Lys Tyr Ser Tyr Arg Pro Thr Asp Ala Glu Lys Gly Gly Phe Gly Val Phe Ala Gly Lys Lys Glu Gln Asp Gly Ser Gly Gly Gly Gly Ala Thr Tyr Lys Val Asp Glu Tyr His Ala Asn Ala Arg Phe Ala Ile Asp His Phe Asn Thr Ser Thr Asn Val Gly Gly Phe Tyr Gly Leu Thr Gly Ser Val Glu Phe Asp Gln Ala Lys Arg Asp Gly Lys Ile Asp Ile Thr Ile Pro Val Ala Asn Leu Gln Ser Gly Ser Gln His Phe Thr Asp His Leu Lys Ser Ala Asp Ile Phe Asp Ala Ala Gln Tyr Pro Asp Ile Arg Phe Val Ser Thr Lys Phe Asn Phe Asn Gly Lys Lys Leu Val Ser Val Asp Gly Asn Leu Thr Met His Gly Lys Thr Ala Pro Val Lys Leu Lys Ala Glu Lys Phe Asn Cys Tyr Gln Ser Pro Met Ala Lys Thr Glu Val Cys Gly Gly Asp Phe Ser Thr Thr Ile Asp Arg Thr Lys Trp Gly Val Asp Tyr Leu Val Asn Val Gly Met Thr Lys Ser Val Arg Ile Asp Ile Gln Ile Glu Ala Ala Lys Gln <210> 13 <211> 2583 <212> DNA
<213> Artificial Sequence <220>
<223> deltaG287NZ-961 <400> 13 atggctagcc ccgatgtcaa gtcggcggac acgctgtcaa aacctgccgc ccctgttgtt 60 tctgaaaaag agacagaggc aaaggaagat gcgccacagg caggttctca aggacagggc 120 gcgccatccg cacaaggcgg tcaagatatg gcggcggttt cggaagaaaa tacaggcaat 180 ggcggtgcgg cacaaacgga caaacccaaa aatgaagacg agggggcgca aaatgatatg 240 ccgcaaaatg ccgccgatac agatagtttg acaccgaatc acaccccggc ttcgaatatg 300 ccggccggaa atatggaaaa ccaagcaccg gatgccgggg aattggagca gccggcaaac 360 caaccggata tggcaaatac ggcggacgga atgcagggtg acgatccgtc ggcagtgggg 420 gaaaatgccg gcaatacggc tgcccaaggt acaaatcaag ccgaaaacaa tcaaaccgcc 480 ggttctcaaa atcctgcctc ttcaaccaat cctagcgcca cgaatagcgg tggtgatttt 540 ggaaggacga acgtgggcaa ttctgttgtg attgacgggc cgtcgcaaaa tataacgttg 600 acccactgta aaggcgattc ttgtagtggc aataatttct tggatgaaga agtacagcta 660 aaatcagaat ttgaaaaatt aagtgatgca gacaaaataa gtaattacaa gaaagatggg 720 aataatgacg ggaagaatga taaatttgtc ggtttggttg ccgatagtgt gcagatgaag 780 ggaatcaatc aatatattat cttttataaa cctaaaccca cttcatttgc gcgatttagg 840 cgttctgcac ggtcgaggcg gtcgcttccg gccgagatgc cgctgattcc cgtcaataag 900 gcggatacgc tgattgtcga tggggaagcg gtcagcctga cggggcattc cggcaatatc 960 ttcgcgcccg aagggaatta ccggtatctg acttacgggg cggaaaaatt gcccggcgga 1020 tcgtatgccc tccgtgttca aggcgaacct tcaaaaggcg aaatgctcgc gggcacggca 1080 gtgtacaacg gcgaagtgct gcattttcat acggaaaacg gccgtccgtc cccgtccaga 1140 ggcaggtttg ccgcaaaagt cgatttcggc agcaaatctg tggacggcat tatcgacagc 1200 ggcgatggtt tgcatatggg tacgcaaaaa ttaaaagccg ccatcgatgg aaacggcttt 1260 aaggggactt ggacggaaaa tggcggcggg gatgtttccg gaaagtttta cggcccggcc 1320 ggcgaggaag tggcggaaaa atacagctat cgcccaacag atgcggaaaa gggcggattc 1380 ggcgtgtttg ccggcaaaaa agagcaggat ggatccggag gaggaggagc cacaaacgac 1440 gacgatgtta aaaaagctgc cactgtggcc attgctgctg cctacaacaa tggccaagaa 1500 atcaacggtt tcaaagctgg agagaccatc tacgacattg atgaagacgg cacaattacc 1560 aaaaaagacg caactgcagc cgatgttgaa gccgacgact ttaaaggtct gggtctgaaa 1620 aaagtcgtga ctaacctgac caaaaccgtc aatgaaaaca aacaaaacgt cgatgccaaa 1680 gtaaaagctg cagaatctga aatagaaaag ttaacaacca agttagcaga cactgatgcc 1740 gctttagcag atactgatgc cgctctggat gcaaccacca acgccttgaa taaattggga 1800 gaaaatataa cgacatttgc tgaagagact aagacaaata tcgtaaaaat tgatgaaaaa 1860 ttagaagccg tggctgatac cgtcgacaag catgccgaag cattcaacga tatcgccgat 1920 tcattggatg aaaccaacac taaggcagac gaagccgtca aaaccgccaa tgaagccaaa 1980 cagacggccg aagaaaccaa acaaaacgtc gatgccaaag taaaagctgc agaaactgca 2040 gcaggcaaag ccgaagctgc cgctggcaca gctaatactg cagccgacaa ggccgaagct 2100 gtcgctgcaa aagttaccga catcaaagct gatatcgcta cgaacaaaga taatattgct 2160 aaaaaagcaa acagtgccga cgtgtacacc agagaagagt ctgacagcaa atttgtcaga 2220 attgatggtc tgaacgctac taccgaaaaa ttggacacac gcttggcttc tgctgaaaaa 2280 tccattgccg atcacgatac tcgcctgaac ggttt.ggata aaacagtgtc agacctgcgc 2340 aaagaaaccc gccaaggcct tgcagaacaa gccgcgctct ccggtctgtt ccaaccttac 2400 aacgtgggtc ggttcaatgt aacggctgca gtcggcggct acaaatccga atcggcagtc 2460 gccatcggta ccggcttccg ctttaccgaa aactttgccg ccaaagcagg cgtggcagtc 2520 ggcacttcgt ccggttcttc cgcagcctac catgtcggcg tcaattacga gtggtaaaag 2580 ctt 2583 <210> 14 <211> 858 <212> PRT
<213> Artificial Sequence <220>
<223> deltaG287NZ-961 <400> 14 Met Ala Ser Pro Asp Val Lys Ser Ala Asp Thr Leu Ser Lys Pro Ala Ala Pro Val Val Ser Glu Lys Glu Thr Glu Ala Lys Glu Asp Ala Pro Gln Ala Gly Ser Gln Gly Gln Gly Ala Pro Ser Ala Gln Gly Gly Gln Asp Met Ala Ala Val Ser Glu Glu Asn Thr Gly Asn Gly Gly Ala Ala Ala Thr Asp Lys Pro Lys Asn Glu Asp Glu Gly Ala Gln Asn Asp Met Pro Gln Asn Ala Ala Asp Thr Asp Ser Leu Thr Pro Asn His Thr Pro Ala Ser Asn Met Pro Ala Gly Asn Met Glu Asn Gln Ala Pro Asp Ala Gly Glu Ser Glu Gln Pro Ala Asn Gln Pro Asp Met Ala Asn Thr Ala Asp Gly Met Gln Gly Asp Asp Pro Ser Ala Gly Gly Glu Asn Ala Gly Asn Thr Ala Ala Gln Gly Thr Asn Gln Ala Glu Asn Asn Gln Thr Ala Gly Ser Gln Asn Pro Ala Ser Ser Thr Asn Pro Ser Ala Thr Asn Ser Gly Gly Asp Phe Gly Arg Thr Asn Val Gly Asn Ser Val Val Ile Asp Gly Pro Ser Gln Asn Ile Thr Leu Thr His Cys Lys Gly Asp Ser Cys Ser Gly Asn Asn Phe Leu Asp Glu Glu Val Gln Leu Lys Ser Glu Phe Glu Lys Leu Ser Asp Ala Asp Lys Ile Ser Asn Tyr Lys Lys Asp Gly Lys Asn Asp Gly Lys Asn Asp Lys Phe Val Gly Leu Val Ala Asp Ser Val Gln Met Lys Gly Ile Asn Gln Tyr Ile Ile Phe Tyr Lys Pro Lys Pro Thr Ser Phe Ala Arg Phe Arg Arg Ser Ala Arg Ser Arg Arg Ser Leu Pro Ala Glu Met Pro Leu Ile Pro Val Asn Gln Ala Asp Thr Leu Ile Val Asp Gly Glu Ala Val Ser Leu Thr Gly His Ser Gly Asn Ile Phe Ala Pro Glu Gly Asn Tyr Arg Tyr Leu Thr Tyr Gly Ala Glu Lys Leu Pro Gly Gly Ser Tyr Ala Leu Arg Val Gln Gly Glu Pro Ser Lys Gly Glu Met Leu Ala Gly Thr Ala Val Tyr Asn Gly Glu Val Leu His Phe His Thr Glu Asn Gly Arg Pro Ser Pro Ser Arg Gly Arg Phe Ala Ala Lys Val Asp Phe Gly Ser Lys Ser Val Asp Gly Ile Ile Asp Ser Gly Asp Gly Leu His Met Gly Thr Gln Lys Phe Lys Ala Ala Ile Asp Gly Asn Gly Phe Lys Gly Thr Trp Thr Glu Asn Gly Gly Gly Asp Val Ser Gly Lys Phe Tyr Gly Pro Ala Gly Glu Glu Val Ala Gly Lys Tyr Ser Tyr Arg Pro Thr Asp Ala Glu Lys Gly Gly Phe Gly Val Phe Ala Gly Lys Lys Glu Gln Asp Gly Ser Gly Gly Gly Gly Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile Ala Ala Ala Tyr Asn Asn Gly Gin Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gln Thr Ala Glu Glu Thr Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gln Gly Leu Ala Glu Gln Ala Ala Leu Ser Gly Leu Phe Gln Pro Tyr Asn Val Gly Arg Phe Asn Val Thr Ala Ala Val Gly Gly Tyr Lys Ser Glu Ser Ala Val Ala Ile Gly Thr Gly Phe Arg Phe Thr Glu Asn Phe Ala Ala Lys Ala Gly Val Ala Val Gly Thr Ser Ser Gly Ser Ser Ala Ala Tyr His Val Gly Val Asn Tyr Glu Trp <210> 15 <211> 1082 <212> PRT
<213> Artificial Sequence <220>
<223> 983 <400> 15 Met Arg Thr Thr Pro Thr Phe Pro Thr Lys Thr Phe Lys Pro Thr Ala Met Ala Leu Ala Val Ala Thr Thr Leu Ser Ala Cys Leu Gly Gly Gly Gly Gly Gly Thr Ser Ala Pro Asp Phe Asn Ala Gly Gly Thr Gly Ile Gly Ser Asn Ser Arg Ala Thr Thr Ala Lys Ser Ala Ala Val Ser Tyr Ala Gly Ile Lys Asn Glu Met Cys Lys Asp Arg Ser Met Leu Cys Ala Gly Arg Asp Asp Val Ala Val Thr Asp Arg Asp Ala Lys Ile Asn Ala Pro Pro Pro Asn Leu His Thr Gly Asp Phe Pro Asn Pro Asn Asp Ala Tyr Lys Asn Leu Ile Asn Leu Lys Pro Ala Ile Glu Ala Gly Tyr Thr Gly Arg Gly Val Glu Val Gly Ile Val Asp Thr Gly Glu Ser Val Gly Ser Ile Ser Phe Pro Glu Leu Tyr Gly Arg Lys Glu His Gly Tyr Asn Glu Asn Tyr Lys Asn Tyr Thr Ala Tyr Met Arg Lys Glu Ala Pro Glu Asp Gly Gly Gly Lys Asp Ile Glu Ala Ser Phe Asp Asp Glu Ala Val Ile Glu Thr Glu Ala Lys Pro Thr Asp Ile Arg His Val Lys Glu Ile Gly His Ile Asp Leu Val Ser His Ile Ile Gly Gly Arg Ser Val Asp Gly Arg Pro Ala Gly Gly Ile Ala Pro Asp Ala Thr Leu His Ile Met Asn Thr Asn Asp Glu Thr Lys Asn Glu Met Met Val Ala Ala Ile Arg Asn Ala Trp Val Lys Leu Gly Glu Arg Gly Val Arg Ile Val Asn Asn Ser Phe Gly Thr Thr Ser Arg Ala Gly Thr Ala Asp Leu Phe Gln Ile Ala Asn Ser Glu Glu Gln Tyr Arg Gln Ala Leu Leu Asp Tyr Ser Gly Gly Asp Lys Thr Asp Glu Gly Ile Arg Leu Met Gln Gln Ser Asp Tyr Gly Asn Leu Ser Tyr His Ile Arg Asn Lys Asn Met Leu Phe :Ile Phe Ser Thr Gly Asn Asp Ala Gln Ala Gln Pro Asn Thr Tyr Ala Leu Leu Pro Phe Tyr Glu Lys Asp Ala Gln Lys Gly Ile Ile Thr Val Ala Gly Val Asp Arg Ser Gly Glu Lys Phe Lys Arg Glu Met Tyr Gly Glu Pro Gly Thr Glu Pro Leu Glu Tyr Gly Ser Asn His Cys Gly Ile Thr Ala Met Trp Cys Leu Ser Ala Pro Tyr Glu Ala Ser Val Arg Phe Thr Arg Thr Asn Pro Ile Gln Ile Ala Gly Thr Ser Phe Ser Ala Pro Ile Val Thr Gly Thr Ala Ala Leu Leu Leu Gln Lys Tyr Pro Trp Met Ser Asn Asp Asn Leu Arg Thr Thr Leu Leu Thr Thr Ala Gln Asp Ile Gly Ala Val Gly Val Asp Ser Lys Phe Gly Trp Gly Leu Leu Asp Ala Gly Lys Ala Met Asn Gly Pro Ala Ser Phe Pro Phe Gly Asp Phe Thr Ala Asp Thr Lys Gly Thr Ser Asp Ile Ala Tyr Ser Phe Arg Asn Asp Ile Ser Gly Thr Gly Gly Leu Ile Lys Lys Gly Gly Ser Gln Leu Gln Leu His Gly Asn Asn Thr Tyr Thr Gly Lys Thr Ile Ile Glu Gly Gly Ser Leu Val Leu Tyr Gly Asn Asn Lys Ser Asp Met Arg Val Glu Thr Lys Gly Ala Leu Ile Tyr Asn Gly Ala Ala Ser Gly Gly Ser Leu Asn Ser Asp Gly Ile Val Tyr Leu Ala Asp Thr Asp Gln Ser Gly Ala Asn Glu Thr Val His Ile Lys Gly Ser Leu Gln Leu Asp Gly Lys Gly Thr Leu Tyr Thr Arg Leu Gly Lys Leu Leu Lys Val Asp Gly Thr Ala Ile Ile Gly Gly Lys Leu Tyr Met Ser Ala Arg Gly Lys Gly Ala Gly Tyr Leu Asn Ser Thr Gly Arg Arg Val Pro Phe Leu Ser Ala Ala Lys Ile Gly Gln Asp Tyr Ser Phe Phe Thr Asn Ile Glu Thr Asp Gly Gly Leu Leu Ala Ser Leu Asp Ser Val Glu Lys Thr Ala Gly Ser Glu Gly Asp Thr Leu Ser Tyr Tyr Val Arg Arg Gly Asn Ala Ala Arg Thr Ala Ser Ala Ala Ala His Ser Ala Pro Ala Gly Leu Lys His Ala Val Glu Gln Gly Gly Ser Asn Leu Glu Asn Leu Met Val Glu Leu Asp Ala Ser Glu Ser Ser Ala Thr Pro Glu Thr Val Glu Thr Ala Ala Ala Asp Arg Thr Asp Met Pro Gly Ile Arg Pro Tyr Gly Ala Thr Phe Arg Ala Ala Ala Ala Val Gln His Ala Asn Ala Ala Asp Gly Val Arg Ile Phe Asn Ser Leu Ala Ala Thr Val Tyr Ala Asp Ser Thr Ala Ala His Ala Asp Met Gln Gly Arg Arg Leu Lys Ala Val Ser Asp Gly Leu Asp His Asn Gly Thr Gly Leu Arg Val Ile Ala Gln Thr Gin Gln Asp Gly Gly Thr Trp Glu Gln Gly Gly Val Glu Gly Lys Met Arg Gly Ser Thr Gln Thr Val Gly Ile Ala Ala Lys Thr Gly Glu Asn Thr Thr Ala Ala Ala Thr Leu Gly Met Gly Arg Ser Thr Trp Ser Glu Asn Ser Ala Asn Ala Lys Thr Asp Ser Ile Ser Leu Phe Ala Gly Ile Arg His Asp Ala Gly Asp Ile Gly Tyr Leu Lys Gly Leu Phe Ser Tyr Gly Arg Tyr Lys Asn Ser Ile Ser Arg Ser Thr Gly Ala Asp Glu His Ala Glu Gly Ser Val Asn Gly Thr Leu Met Gln Leu Gly Ala Leu Gly Gly Val Asn Val Pro Phe Ala Ala Thr Gly Asp Leu Thr Val Glu Gly Gly Leu Arg Tyr Asp Leu Leu Lys Gln Asp Ala Phe Ala Glu Lys Gly Ser Ala Leu Gly Trp Ser Gly Asn Ser Leu Thr Glu Gly Thr Leu Val Gly Leu Ala Gly Leu Lys Leu Ser Gln Pro Leu Ser Asp Lys Ala Val Leu Phe Ala Thr Ala Gly Val Glu Arg Asp Leu Asn Gly Arg Asp Tyr Thr Val Thr Gly Gly Phe Thr Gly Ala Thr Ala Ala Thr Gly Lys Thr Sly Ala Arg Asn Met Pro His Thr Arg Leu Val Ala Gly Leu Gly Ala Asp Val Glu Phe Gly Asn Gly Trp Asn Gly Leu Ala Arg Tyr Ser Tyr Ala Gly Ser Lys Gln Tyr Gly Asn His Ser Gly Arg Val Gly Val Gly Tyr Arg Phe <210> 16 <211> 1047 <212> PRT
<213> Artificial Sequence <220>
<223> deltaG983 <400> 16 Thr Ser Ala Pro Asp Phe Asn Ala Gly Gly Thr Gly Ile Gly Ser Asn Ser Arg Ala Thr Thr Ala Lys Ser Ala Ala Val Ser Tyr Ala Gly Ile Lys Asn Glu Met Cys Lys Asp Arg Ser Met Leu Cys Ala Gly Arg Asp Asp Val Ala Val Thr Asp Arg Asp Ala Lys Ile Asn Ala Pro Pro Pro Asn Leu His Thr Gly Asp Phe Pro Asn Pro Asn Asp Ala Tyr Lys Asn Leu Ile Asn Leu Lys Pro Ala Ile Glu Ala Gly Tyr Thr Gly Arg Gly Val Glu Val Gly Ile Val Asp Thr Gly Glu Ser Val Gly Ser Ile Ser Phe Pro Glu Leu Tyr Gly Arg Lys Glu His Gly Tyr Asn Glu Asn Tyr Lys Asn Tyr Thr Ala Tyr Met Arg Lys Glu Ala Pro Glu Asp Gly Gly Gly Lys Asp Ile Glu Ala Ser Phe Asp Asp Glu Ala Val Ile Glu Thr Glu Ala Lys Pro Thr Asp Ile Arg His Val Lys Glu Ile Gly His Ile Asp Leu Val Ser His Ile Ile Gly Gly Arg Ser Val Asp Gly Arg Pro Ala Gly Gly Ile Ala Pro Asp Ala Thr Leu His Ile Met Asn Thr Asn Asp Glu Thr Lys Asn Glu Met Met Val Ala Ala Ile Arg Asn Ala Trp Val Lys Leu Gly Glu Arg Gly Val Arg Ile Val Asn Asn Ser Phe Gly Thr Thr Ser Arg Ala Gly Thr Ala Asp Leu Phe Gln Ile Ala Asn Ser Glu Glu Gln Tyr Arg Gln Ala Leu Leu Asp Tyr Ser Gly Gly Asp Lys Thr Asp Glu Gly Ile Arg Leu Met Gln Gln Ser Asp Tyr Gly Asn Leu Ser Tyr His Ile Arg Asn Lys Asn Met Leu Phe Ile Phe Ser Thr Gly Asn Asp Ala Gln Ala Gln Pro Asn Thr Tyr Ala Leu Leu Pro Phe Tyr Glu Lys Asp Ala Gin Lys Gly Ile Ile Thr Val Ala Gly Val Asp Arg Ser Gly Glu Lys Phe Lys Arg Glu Met Tyr Gly Glu Pro Gly Thr Glu Pro Leu Glu Tyr Gly Ser Asn His Cys Gly Ile Thr Ala Met Trp Cys Leu Ser Ala Pro Tyr Glu Ala Ser Val Arg Phe Thr Arg Thr Asn Pro Ile Gln Ile Ala Gly Thr Ser Phe Ser Ala Pro Ile Val Thr Gly Thr Ala Ala Leu Leu Leu Gln Lys Tyr Pro Trp Met Ser Asn Asp Asn Leu Arg Thr Thr Leu Leu Thr Thr Ala Gln Asp Ile Gly Ala Val Gly Val Asp Ser Lys Phe Gly Trp Gly Leu Leu Asp Ala Gly Lys Ala Met Asn Gly Pro Ala Ser Phe Pro Phe Gly Asp Phe Thr Ala Asp Thr Lys Gly Thr Ser Asp Ile Ala Tyr Ser Phe Arg Asn Asp Ile Ser Gly Thr Gly Gly Leu Ile Lys Lys Gly Gly Ser Gln Leu Gln Leu His Gly Asn Asn Thr Tyr Thr Gly Lys Thr Ile Ile Glu Gly Gly Ser Leu Val Leu Tyr Gly Asn Asn Lys Ser Asp Met Arg Val Glu Thr Lys Gly Ala Leu Ile Tyr Asn Gly Ala Ala Ser Gly Gly Ser Leu Asn Ser Asp Gly Ile Val Tyr Leu Ala Asp Thr Asp Gln Ser Gly Ala Asn Glu Thr Val His Ile Lys Gly Ser Leu Gln Leu Asp Gly Lys Gly Thr Leu Tyr Thr Arg Leu Gly Lys Leu Leu Lys Val Asp Gly Thr Ala Ile Ile Gly Gly Lys Leu Tyr Met Ser Ala Arg Gly Lys Gly Ala Gly Tyr Leu Asn Ser Thr Gly Arg Arg Val Pro Phe Leu Ser Ala Ala Lys Ile Gly Gln Asp Tyr Ser Phe Phe Thr Asn Ile Glu Thr Asp Gly Gly Leu Leu Ala Ser Leu Asp Ser Val Glu Lys Thr Ala Gly Ser Glu Gly Asp Thr Leu Ser Tyr Tyr Val Arg Arg Gly Asn Ala Ala Arg Thr Ala Ser Ala Ala Ala His Ser Ala Pro Ala Gly Leu Lys His Ala Val Glu Gin Gly Gly Ser Asn Leu Glu Asn Leu Met Val Glu Leu Asp Ala Ser Glu Ser Ser Ala Thr Pro Glu Thr Val Glu Thr Ala Ala Ala Asp Arg Thr Asp Met Pro Gly Ile Arg Pro Tyr Gly Ala Thr Phe Arg Ala Ala Ala Ala Val Gln His Ala Asn Ala Ala Asp Gly Val Arg Ile Phe Asn Ser Leu Ala Ala Thr Val Tyr Ala Asp Ser Thr Ala Ala His Ala Asp Met Gln Gly Arg Arg Leu Lys Ala Val Ser Asp Gly Leu Asp His Asn Gly Thr Gly Leu Arg Val Ile Ala Gln Thr Gln Gln Asp Gly Gly Thr Trp Glu Gln Gly Gly Val Glu Gly Lys Met Arg Gly Ser Thr Gln Thr Val Gly Ile Ala Ala Lys Thr Gly Glu Asn Thr Thr Ala Ala Ala Thr Leu Gly Met Gly Arg Ser Thr Trp Ser Glu Asn Ser Ala Asn Ala Lys Thr Asp Ser Ile Ser Leu Phe Ala Gly Ile Arg His Asp Ala Gly Asp Ile Gly Tyr Leu Lys Gly Leu Phe Ser Tyr Gly Arg Tyr Lys Asn Ser Ile Ser Arg Ser Thr Gly Ala Asp Glu His Ala Glu Gly Ser Val Asn Gly Thr Leu Met Gln Leu Gly Ala Leu Gly Gly Val Asn Val Pro Phe Ala Ala Thr Gly Asp Leu Thr Val Glu Gly Gly Leu Arg Tyr Asp Leu Leu Lys Gln Asp Ala Phe Ala Glu Lys Gly Ser Ala Leu Gly Trp Ser Gly Asn Ser Leu Thr Glu Gly Thr Leu Val Gly Leu Ala Gly Leu Lys Leu Ser Gln Pro Leu Ser Asp Lys Ala Val Leu Phe Ala Thr Ala Gly Val Glu Arg Asp Leu Asn Gly Arg Asp Tyr Thr Val Thr Gly Gly Phe Thr Gly Ala Thr Ala Ala Thr Gly Lys Thr Gly Ala Arg Asn Met Pro His Thr Arg Leu Val Ala Gly Leu Gly Ala Asp Val Glu Phe Gly Asn Gly Trp Asn Gly Leu Ala Arg Tyr Ser Tyr Ala Gly Ser Lys Gln Tyr Gly Asn His Ser Gly Arg Val Gly Val Gly Tyr Arg Phe <210> 17 <211> 4425 <212> DNA
<213> Artificial Sequence <220>
<223> deltaG983-0RF46.1 <400> 17 atgacttctg cgcccgactt caatgcaggc ggtaccggta tcggcagcaa cagcagagca 60 acaacagcga aatcagcagc agtatcttac gccggtatca agaacgaaat gtgcaaagac 120 agaagcatgc tctgtgccgg tcgggatgac gttgcggtta cagacaggga tgccaaaatc 180 aatgcccccc ccccgaatct gcataccgga gactttccaa acccaaatga cgcatacaag 240 aatttgatca acctcaaacc tgcaattgaa gcaggctata caggacgcgg ggtacaggta 300 ggtatcgtcg acacaggcga atccgtcggc agcatatcct ttcccgaact gtatggcaga 360 aaagaacacg gctataacga aaattacaaa aactatacgg cgtatatgcg gaaggaagcg 420 cctgaagacg gaggcggtaa agacattgaa gcttctttcg acgatgaggc cgttatagag 480 actgaagcaa agccgacgga tatccgccac gtaaaagaaa tcggacacat cgatttggtc 540 tcccatatta ttggcgggcg ttccgtggac ggcagacctg caggcggtat tgcgcccgat 600 gcgacgctac acataatgaa tacgaatgat gaaaccaaga acgaaatgat ggttgcagcc 660 atccgcaatg catgggtcaa gctgggcgaa cgtggcgtgc gcatcgtcaa taacagtttt 720 ggaacaacat cgagggcagg cactgccgac cttttccaaa tagccaattc ggaggagcag 780 taccgccaag cgttgctcga ctattccggc ggtgataaaa cagacaaggg tatccgcctg 840 atgcaacaga gcgattacgg caacctgtcc taccacatcc gtaataaaaa catgcttttc 900 atcttttcga caggcaatga cgcacaagct cagcccaaca catatgccct attgccattt 960 tatgaaaaag acgctcaaaa aggcattatc acagtcgcag gcgtagaccg cagtggagaa 1020 aagttcaaac gggaaatgta tggagaaccg ggtacagaac cgcttgagta tggctccaac 1080 cattgcggaa ttactgccat gtggtgcctg tcggcaccct atgaagcaag cgtccgtttc 1140 acccgtacaa acccgattca aattgccgga acatcctttt ccgcacccat cgtaaccggc 1200 acggcggctc tgctgctgca gaaatacccg tggatgagca acgacaacct gcgtaccacg 1260 ttgctgacga cggctcagga catcggtcca gtcggcgtgg acagcaagtt cggctgggga 1320 ctgctggatg cgggtaaggc catgaaggga cccgcgtcct ttccgttcgg cgactttacc 1380 gccgatacga aaggtacatc cgatattgcc tactccttcc gtaacgacat ttcaggcacg 1440 ggcggcctga tcaaaaaagg cggcagccaa ctgcaactgc acggcaacaa cacctatacg 1500 ggcaaaacca ttatcgaagg cggttcgctg gtgttgtacg gcaacaacaa atcggatatg 1560 cgcgtcgaaa ccaaaggtgc gctgatttat aacggggcgg catccggcgg cagcctgaac 1620 agcgacggca ttgtctatct ggcagatacc gaccaatccg gcgcaaacga aaccgtacac 1680 atcaaaggca gtttgcagct ggacggcaaa ggtacgctgt acacacgttt gggcaaactg 1740 ctgaaagtgg acggtacggc gattatcggc ggcaagctgt acatgtcggc acgcggcaag 1800 ggggcaggct atctcaacag taccggacga cgtgttccct tcctgagtgc cgccaaaatc 1860 gggcaggatt attctttctt cacaaacatc gaaaccgacg gcggcctgct ggcttccctc 1920 gacagcgtcg aaaaaacagc gggcagtaaa ggcgacacgc tgtcctatta tgtccgtcgc 1980 ggcaatgcgg cacggactgc ttcggcagcg gcacattccg cgcccgccgg tctgaaacac 2040 gccgtagaac agggcggcag caatctggaa aacctgatgg tcgaactgga tgcctccgaa 2100 tcatccgcaa cacccgagac ggttgaaact gcggcagccg accgaacaga tatgccgggc 2160 atccgcccct acggcgcaac tttccgcgca gcggcagccg tacagcatgc gaatgccgcc 2220 gacggtgtac gcatcttcaa cagtctcgcc gctaccgtct atgccgacag taccgccgcc 2280 catgccgata tgcagggacg ccgcctgaaa gccgtatcgg acgggttgga ccacaacggc 2340 acgggtctgc gcgtcatcgc gcaaacccaa caggacggtg gaacgtggga acagggcggt 2400 gttgaaggca aaatgcgcgg cagtacccaa accgtcggca ttgccgcgaa aaccggcgaa 2460 aatacgacag cagccgccac actgggcatg ggacgcagca catggagcga aaacagtgca 2520 aatgcaaaaa ccgacagcat tagtctgttt gcaggcaaac ggcacgatgc gggcgatatc 2580 ggctatctca aaggcctgtt ctcctacgga cgctacaaaa acagcatcag ccgcagcacc 2640 ggtgcggacg aacatgcgga aggcagcgtc aacggcacgc tgatgcagct gggcgcactg 2700 ggcggtgtca acgttccgtt tgccgcaacg ggagatttga cggtcgaagg cggtctgcgc 2760 tacgacctgc tcaaacagga tgcattcgcc gaaaaaggca gtgctttggg ctggagcggc 2820 aacagcctca ctgaaggcac gctggtcgga ctcgcgggtc tgaagctgtc gcaacccttg 2880 agcgataaag ccgtcctgtt tgcaacggcg ggcgtggaac gcgacctgaa cggacgcgac 2940 tacacggtaa cgggcggctt taccggcgcg actgcagcaa ccggcaagac gggggcacgc 3000 aatatgccgc acacccgtct ggttgccggc ctgggcgcgg atgtcgaatt cggcaacggc 3060 tggaacggct tggcacgtta cagctacgcc ggttccaaac agtacggcaa ccacagcgga 3120 cgagtcggcg taggctaacg gttcctcgac ggtggcggag gcactggatc ctcagatttg 3180 gcaaacgatt cttttatccg gcaggttctc gaccgtcagc atttcgaacc cgacgggaaa 3240 taccacctat tcggcagcag gggggaactt gccgagcgca gcggccatat cggattggga 3300 aaaatacaaa gccatcagtt gggcaacctg atgattcaac aggcggccat taaaggaaat 3360 atcggctaca ttgtccgctt ttccgatcac gggcacgaag tccattcccc cttcgacaac 3420 catgcctcac attccgattc tgatgaagcc ggtagtcccg ttgacggatt tagcctttac 3480 cgcatccatt gggacggata cgaacaccat cccgccgacg gctatgacgg gccacagggc 3540 ggcggctatc ccgctcccaa aggcgcgagg gatatataca gctacgacat aaaaggcgtt 3600 gcccaaaata tccgcctcaa cctgaccgac aaccgcagca ccggacaacg gcttgccgac 3660 cgtttccaca atgccggtag tatgctgacg caaggagtag gcgacggatt caaacgcgcc 3720 acccgataca gccccgagct ggacagatcg ggcaatgccg ccgaagcctt caacggcact 3780 gcagatatcg ttaaaaacat catcggcgcg gcaggagaaa ttgtcggcgc aggcgatgcc 3840 gtgcagggca taagcgaagg ctcaaacatt gctgtcatgc acggcttggg tctgctttcc 3900 accgaaaaca agatggcgcg catcaacgat ttggcagata tggcgcaact caaagactat 3960 gccgcagcag ccatccgcga ttgggcagtc caaaacccca atgccgcaca aggcatagaa 4020 gccgtcagca atatctttat ggcagccatc cccatcaaag ggattggagc tgttcgggga 4080 aaatacggct tgggcggcat cacggcacat cctatcaagc ggtcgcagat gggcgcgatc 4140 gcattgccga aagggaaatc cgccgtcagc gacaattttg ccgatgcggc atacgccaaa 4200 tacccgtccc cttaccattc ccgaaatatc cgttcaaact tggagcagcg ttacggcaaa 4260 gaaaacatca cctcctcaac cgtgccgccg tcaaacggca aaaatgtcaa actgccagac 4320 caacgccacc cgaagacagg cgtaccgttt gacggtaaag ggtttccgaa ttttgagaag 4380 cacgtgaaat atgatacgct cgagcaccac caccaccacc actga 4425 <210> 18 <211> 1474 <212> PRT
<213> Artificial Sequence <220>
<223> deltaG983-ORF46.1 <400> 18 Met Thr Ser Ala Pro Asp Phe Asn Ala Gly Gly Thr Gly Ile Gly Ser Asn Ser Arg Ala Thr Thr Ala Lys Ser Ala Ala Val Ser Tyr Ala Gly Ile Lys Asn Glu Met Cys Lys Asp Arg Ser Met Leu Cys Ala Gly Arg Asp Asp Val Ala Val Thr Asp Arg Asp Ala Lys Ile Asn Ala Pro Pro Pro Asn Leu His Thr Gly Asp Phe Pro Asn Pro Asn Asp Ala Tyr Lys Asn Leu Ile Asn Leu Lys Pro Ala Ile Glu Ala Gly Tyr Thr Gly Arg Gly Val Glu Val Gly Ile Val Asp Thr Gly Glu Ser Val Gly Ser Ile Ser Phe Pro Glu Leu Tyr Gly Arg Lys Glu His Gly Tyr Asn Glu Asn Tyr Lys Asn Tyr Thr Ala Tyr Met Arg Lys Glu Ala Pro Glu Asp Gly Gly Gly Lys Asp Ile Glu Ala Ser Phe Asp Asp Glu Ala Val Ile Glu Thr Glu Ala Lys Pro Thr Asp Ile Arg His Val Lys Glu Ile Gly His Ile Asp Leu Val Ser His Ile Ile Gly Gly Arg Ser Val Asp Gly Arg Pro Ala Gly Gly Ile Ala Pro Asp Ala Thr Leu His Ile Met Asn Thr Asn Asp Glu Thr Lys Asn Glu Met Met Val Ala Ala Ile Arg Asn Ala Trp Val Lys Leu Gly Glu Arg Gly Val Arg Ile Val Asn Asn Ser Phe Gly Thr Thr Ser Arg Ala Gly Thr Ala Asp Leu Phe Gln Ile Ala Asn Ser Glu Glu Gln Tyr Arg Gln Ala Leu Leu Asp Tyr Ser Gly Gly Asp Lys Thr Asp Glu Gly Ile Arg Leu Met Gln Gln Ser Asp Tyr Gly Asn Leu Ser Tyr His Ile Arg Asn Lys Asn Met Leu Phe Ile Phe Ser Thr Gly Asn Asp Ala Gln Ala Gln Pro Asn Thr Tyr Ala Leu Leu Pro Phe Tyr Glu Lys Asp Ala Gln Lys Gly Ile Ile Thr Val Ala Gly Val Asp Arg Ser Gly Glu Lys Phe Lys Arg Glu Met Tyr Gly Glu Pro Gly Thr Glu Pro Leu Glu Tyr Gly Ser Asn His Cys Gly Ile Thr Ala Met Trp Cys Leu Ser Ala Pro Tyr Glu Ala Ser Val Arg Phe Thr Arg Thr Asn Pro Ile Gln Ile Ala Gly Thr Ser Phe Ser Ala Pro Ile Val Thr Gly Thr Ala Ala Leu Leu Leu Gln Lys Tyr Pro Trp Met Ser Asn Asp Asn Leu Arg Thr Thr Leu Leu Thr Thr Ala Gln Asp Ile Gly Ala Val Gly Val Asp Ser Lys Phe Gly Trp Gly Leu Leu Asp Ala Gly Lys Ala Met Asn Gly Pro Ala Ser Phe Pro Phe Gly Asp Phe Thr Ala Asp Thr Lys Gly Thr Ser Asp Ile Ala Tyr Ser Phe Arg Asn Asp Ile Ser Gly Thr Gly Gly Leu Ile Lys Lys Gly Gly Ser Gln Leu Gln Leu His Gly Asn Asn Thr Tyr Thr Gly Lys Thr Ile Ile Glu Gly Gly Ser Leu Val Leu Tyr Gly Asn Asn Lys Ser Asp Met Arg Val Glu Thr Lys Gly Ala Leu Ile Tyr Asn Gly Ala Ala Ser Gly Gly Ser Leu Asn Ser Asp Gly Ile Val Tyr Leu Ala Asp Thr Asp Gln Ser Gly Ala Asn Glu Thr Val His Ile Lys Gly Ser Leu Gln Leu Asp Gly Lys Gly Thr Leu Tyr Thr Arg Leu Gly Lys Leu Leu Lys Val Asp Gly Thr Ala Ile Ile Gly Gly Lys Leu Tyr Met Ser Ala Arg Gly Lys Gly Ala Gly Tyr Leu Asn Ser Thr Gly Arg Arg Val Pro Phe Leu Ser Ala Ala Lys Ile Gly Gln Asp Tyr Ser Phe Phe Thr Asn Ile Glu Thr Asp Gly Gly Leu Leu Ala Ser Leu Asp Ser Val Glu Lys Thr Ala Gly Ser Glu Gly Asp Thr Leu Ser Tyr Tyr Val Arg Arg Gly Asn Ala Ala Arg Thr Ala Ser Ala Ala Ala His Ser Ala Pro Ala Gly Leu Lys His Ala Val Glu Gln Gly Gly Ser Asn Leu Glu Asn Leu Met Val Glu Leu Asp Ala Ser Glu Ser Ser Ala Thr Pro Glu Thr Val Glu Thr Ala Ala Ala Asp Arg Thr Asp Met Pro Gly Ile Arg Pro Tyr Gly Ala Thr Phe Arg Ala Ala Ala Ala Val Gin His 725 730 '735 Ala Asn Ala Ala Asp Gly Val Arg Ile Phe Asn Ser Leu Ala Ala Thr Val Tyr Ala Asp Ser Thr Ala Ala His Ala Asp Met Gln Gly Arg Arg Leu Lys Ala Val Ser Asp Gly Leu Asp His Asn Gly Thr Gly Leu Arg Val Ile Ala Gln Thr Gln Gln Asp Gly Gly Thr Trp Glu Gln Gly Gly Val Glu Gly Lys Met Arg Gly Ser Thr Gln Thr Val Gly Ile Ala Ala Lys Thr Gly Glu Asn Thr Thr Ala Ala Ala Thr Leu Gly Met Gly Arg Ser Thr Trp Ser Glu Asn Ser Ala Asn Ala Lys Thr Asp Ser Ile Ser Leu Phe Ala Gly Ile Arg His Asp Ala Gly Asp Ile Gly Tyr Leu Lys Gly Leu Phe Ser Tyr Gly Arg Tyr Lys Asn Ser Ile Ser Arg Ser Thr Gly Ala Asp Glu His Ala Glu Gly Ser Val Asn Gly Thr Leu Met Gln Leu Gly Ala Leu Gly Gly Val Asn Val Pro Phe Ala Ala Thr Gly Asp Leu Thr Val Glu Gly Gly Leu Arg Tyr Asp Leu Leu Lys Gln Asp Ala Phe Ala Glu Lys Gly Ser Ala Leu Gly Trp Ser Gly Asn Ser Leu Thr Glu Gly Thr Leu Val Gly Leu Ala Gly Leu Lys Leu Ser Gln Pro Leu Ser Asp Lys Ala Val Leu Phe Ala Thr Ala Gly Val Glu Arg Asp Leu Asn Gly Arg Asp Tyr Thr Val Thr Gly Gly Phe Thr Gly Ala Thr Ala Ala Thr Gly Lys Thr Gly Ala Arg Asn Met Pro His Thr Arg Leu Val Ala Gly Leu Gly Ala Asp Val Glu Phe Gly Asn Gly Trp Asn Gly Leu Ala Arg Tyr Ser Tyr Ala Gly Ser Lys Gin Tyr Gly Asn His Ser Gly Arg Val Gly Val Gly Tyr Arg Phe Leu Asp Gly Gly Gly Gly Thr Gly Ser Ser Asp Leu Ala Asn Asp Ser Phe Ile Arg Gin Val Leu Asp Arg Gin His Phe Glu Pro Asp Gly Lys Tyr His Leu Phe Gly Ser Arg Gly Glu Leu Ala Glu Arg Ser Gly His Ile Gly Leu Gly Lys Ile Gin Ser His Gin Leu Gly Asn Leu Met Ile Gin Gin Ala Ala Ile Lys Gly Asn 1105 113.0 1115 1120 Ile Gly Tyr Ile Val Arg Phe Ser Asp His Gly His Glu Val His Ser Pro Phe Asp Asn His Ala Ser His Ser Asp Ser Asp Glu Ala Gly Ser Pro Val Asp Gly Phe Ser Leu Tyr Arg Ile His Trp Asp Gly Tyr Glu His His Pro Ala Asp Gly Tyr Asp Gly Pro Gin Gly Gly Gly Tyr Pro Ala Pro Lys Gly Ala Arg Asp Ile Tyr Ser Tyr Asp Ile Lys Gly Val Ala Gin Asn Ile Arg Leu Asn Leu Thr Asp Asn Arg Ser Thr Gly Gin Arg Leu Ala Asp Arg Phe His Asn Ala G1y Ser Net Leu Thr Gin Gly Val Gly Asp Gly Phe Lys Arg Ala Thr Arg Tyr Ser Pro Glu Leu Asp Arg Ser Gly Asn Ala Ala Glu Ala Phe Asn Gly Thr Ala Asp Ile Val Lys Asn Ile Ile Gly Ala Ala Gly Glu Ile Val Gly Ala Gly Asp Ala Val Gln Gly Ile Ser Glu Gly Ser Asn Ile Ala Val Met His Gly Leu Gly Leu Leu Ser Thr Glu Asn Lys Met Ala Arg Ile Asn Asp Leu Ala Asp Met Ala Gln Leu Lys Asp Tyr Ala Ala Ala Ala Ile Arg Asp Trp Ala Val Gln Asn Pro Asn Ala Ala Gln Gly Ile Glu Ala Val Ser Asn Ile Phe Met Ala Ala Ile Pro Ile Lys Gly Ile Gly Ala Val Arg Gly Lys Tyr Gly Leu Gly Gly Ile Thr Ala His Pro Ile Lys Arg Ser Gln 1365 1370 :L375 Met Gly Ala Ile Ala Leu Pro Lys Gly Lys Ser Ala Val Ser Asp Asn Phe Ala Asp Ala Ala Tyr Ala Lys Tyr Pro Ser Pro Tyr His Ser Arg Asn Ile Arg Ser Asn Leu Glu Gln Arg Tyr Gly Lys Glu Asn Ile Thr Ser Ser Thr Val Pro Pro Ser Asn Gly Lys Asn Val Lys Leu Ala Asp Gln Arg His Pro Lys Thr Gly Val Pro Phe Asp Gly Lys Gly Phe Pro Asn Phe Glu Lys His Val Lys Tyr Asp Thr Leu Glu His His His His His His <210> 19 <211> 3939 <212> DNA
<213> Artificial Sequence <220>
<223> deltaG983-741 <400> 19 atgacttctg cgcccgactt caatgcaggc ggtaccggta tcggcagcaa cagcagagca 60 acaacagcga aatcagcagc agtatcttac gccggtatca agaacgaaat gtgcaaagac 120 agaagcatgc tctgtgccgg tcgggatgac gttgcggtta cagacaggga tgccaaaatc 180 aatgcccccc ccccgaatct gcataccgga gactttccaa acccaaatga cgcatacaag 240 aatttgatca acctcaaacc tgcaattgaa gcaggctata caggacgcgg ggtagaggta 300 ggtatcgtcg acacaggcga atccgtcggc agcatatcct ttcccgaact gtatggcaga 360 aaagaacacg gctataacga aaattacaaa aactatacgg cgtatatgcg gaaggaagcg 420 cctgaagacg gaggcggtaa agacattgaa gcttctttcg acgatgaggc cgttatagag 480 actgaagcaa agccgacgga tatccgccac gtaaaagaaa tcggaaccat cgatttggtc 540 tcccatatta ttggcgggcg ttccgtggac ggcagacctg caggcggtat tgcgcccgat 600 gcgacgctac acataatgaa tacgaatgat gaaaccaaga acgaaatgat ggttgcagcc 660 atccgcaatg catgggtcaa gctgggcgaa cgtggcgtgc gcatcgtcaa taacagtttt 720 ggaacaacat cgagggcagg cactgccgac cttttccaaa tagccaattc gaaggaagag 780 taccgccaag cgttgctcga ctattccggc ggtgataaaa cagacgaggg tatccgcctg 840 atgcaacaga gcgattacgg caacctgtcc tactacatcc gtaataaaaa catgcttttc 900 atcttttcga caggcaatga cgcacaagct cagcccaaca catatgccct attgccattt 960 tatgaaaaag acgctcaaaa aggcattatc acagtcgcag gcgtagaccg cagtggagaa 1020 aagttcaaac gggaaatgta tggagaaccg ggtacagaac cgcttgagta tggctccaac 1080 cattgcggaa ttactgccat gtggtgcctg tcggcaccct atgaagcaag cgtccgtttc 1140 acccgtacaa acccgattca aattgccgga acatcctttt ccgcacccat cgtaaccggc 1200 acggcggctc tgctgctgca gaaatacccg tggatgagca acgacaacct gcgtaccacg 1260 ttgctgacga cggctcagga catcggtgca gtcggcgtgg acagcaagtt cggctgggga 1320 ctgctggatg cgggtaaggc catgaacgga cccgcgtcct ttccgttcgg cgactttacc 1380 gccgatatca aaggtacatc cgatattgcc tactccttcc gtaacgacat ttcaggcacg 1440 ggcggcctga tcaaaaaagg cggcagccaa ctgcaactgc acggcaacaa cacctatacg 1500 ggaaaaacca ttatcgaagg cggtttgctg gtgttgtacg gcaacaacaa atcggatatg 1560 cgcgtcgaaa ccaaaggtgc gctgatttat aacggggcgg catccggcgg cagcctgaac 1620 agcgacggca ttgtctatct ggcagatacc gaccaatccg gcgcaaacga aaccgtacac 1680 atcaaaggca gtctgcagct ggacggcaaa ggtacgctgt acacagtttt gggcaaactg 1740 ctgaaagtgg acggtacggc gattatcggc ggcaagctgt acatgtcggc acgcggcaag 1800 ggggcaggct atctcaacag taccggacga cgtgttccct tcctgagtgc cgccaaaatc 1860 gggcaggatt attctttctt cacaaacatc gaaaccgacg gcggcctgct ggcttccctc 1920 gacagcgtcg aaaaaacagc gggcagtgaa ggcgacacgc tgtcctatta tgtccgtcgc 1980 ggcaatctgg cacggactgc ttcggcagcg gcacattccg cgcccgccgg tctgaaacac 2040 gccgtagaac agggcggcag caatctggaa aacctgatgg tcgaactgga tgcctccgaa 2100 tcatccgcaa cacccgagac ggttgaaact gcggcagccg accgcacaga tatgccgggc 2160 atccgcccct acggcgcaac tttccgcgca gcggcagccg tacagcatgc gaatgccgcc 2220 gacggtgtac gcatcttcaa cagtctcgcc gctaccgtct atgccgacag taccgccgcc 2280 catgccgata tgcagggacg ccgcctgaaa gccgtatcgg acgggttgga ccaaaacggc 2340 acgggtctgc gcgtcatcgc gcaaacccaa caggacggtg gaacgtggga acagggcggt 2400 gttgaaggca aaatgcgcgg cagtacccaa accgtcggca ttgccgcgaa aaccggagaa 2460 aatacgacag cagccgccac actgggcatg ggacgcagca catggagcga aaacagtgca 2520 aatgcaaaaa ccgacagcat tagtctgttt gcaggcatac ggcacgatgc gggcgatatc 2580 ggctatctca aaggcctgtt ctcctacgga cgctacaaaa acagcatcag ccgcagcacc 2640 ggtgcggacg aacatgcgga aggcagcgtc aacggcacgc tgatgcagct gggcgcactg 2700 ggcggtgtca acgttccgtt tgccgcaacg ggagatttga cggtcgaagg cggtctgcgc 2760 tacgacctgc tcaaacagga tgcattcgcc gaaaaaggca gtgctttggg ctggagcggc 2820 aacagcctca ctgaaggcac gctggtcgga ctcgcgggtc tgaagctgtc gcaacccttg 2880 agcgataaag ccgtcctgtt tgcaacggcg ggcgtggaac gcgacctgaa cggacgcgac 2940 tacacggtaa cgggcggctt taccggcgcg actgcagcaa ccggcaagac gggggcacgc 3000 aatatgccgc acacccgtct ggttgccggc ctgggcgcgg atgtcgaatt cggcaacggc 3060 tggaacggct tggcacgtta cagctacgcc ggttccaaac agtacggcaa ccacagcgga 3120 cgagtcgccg taggctaccg gttcctcgag ggatccggag ggggtggtgt cgccgccgac 3180 atcggtgcgg ggcttgccga tgcactaacc gcaacgctag accataaaga caaaggtttg 3240 cagtctttga cgctggatca gtccgtcagg aaaaacgaga aactgaagct qgcggcacaa 3300 ggtgcggaaa aaacttatgg aaacggtgac agcctcaata cgggcaaatt gaagaacgac 3360 aaggtcagcc gtttcgactt tatccgccaa atcgaagtgg acgggcagct cattaccttg 3420 gagagtggag agttccaagt atacaaacaa agccattccg ccttaaccgc ctttcagacc 3480 gagcaaatac aagattcgga gcattccggg aagatggttg cgaaacgcca gttcagaatc 3540 ggcgacatag cgggcgaaca tacatctttt gacaagcttc ccgaaggcgg cagggcgaca 3600 tatcgcggga cggcgttcgg ttcagacgat gccggcggaa aactgaccta caccatagat 3660 ttcgccgcca agcagggaaa cggcaaaatc gaacatttga aatcgccaga actcaatgtc 3720 gacctggccg ccgccgatat caagccggat ggaaaacgcc atgccgtcat cagcggttcc 3780 gtcctttaca accaagccga gaaaggcagt tactccctcg gtatctttgg cggaaaagcc 3840 caggaagttg ccggcagcgc ggaagtgaaa accgtaaacg gcatacgcca tatcggcctt 3900 gccgccaagc aactcgagca ccaccaccac caccactga 3939 <210> 20 <211> 1312 <212> PRT
<213> Artificial Sequence <220>
<223> deltaG983-741 <400> 20 Met Thr Ser Ala Pro Asp Phe Asn Ala Gly Gly Thr Gly Ile Gly Ser Asn Ser Arg Ala Thr Thr Ala Lys Ser Ala Ala Val Ser Tyr Ala Gly Ile Lys Asn Glu Met Cys Lys Asp Arg Ser Met Leu Cys Ala Gly Arg Asp Asp Val Ala Val Thr Asp Arg Asp Ala Lys Ile Asn Ala Pro Pro Pro Asn Leu His Thr Gly Asp Phe Pro Asn Pro Asn Asp Ala Tyr Lys Asn Leu Ile Asn Leu Lys Pro Ala Ile Glu Ala Gly Tyr Thr Gly Arg Gly Val Glu Val Gly Ile Val Asp Thr Gly Glu Ser Val Gly Ser Ile Ser Phe Pro Glu Leu Tyr Gly Arg Lys Glu His Gly Tyr Asn Glu Asn Tyr Lys Asn Tyr Thr Ala Tyr Met Arg Lys Glu Ala Pro Glu Asp Gly Gly Gly Lys Asp Ile Glu Ala Ser Phe Asp Asp Glu Ala Val Ile Glu Thr Glu Ala Lys Pro Thr Asp Ile Arg His Val Lys Glu Ile Gly His Ile Asp Leu Val Ser His Ile Ile Gly Gly Arg Ser Val Asp Gly Arg Pro Ala Gly Gly Ile Ala Pro Asp Ala Thr Leu His Ile Met Asn Thr Asn Asp Glu Thr Lys Asn Glu Met Met Val Ala Ala Ile Arg Asn Ala Trp Val Lys Leu Gly Glu Arg Gly Val Arg Ile Val Asn Asn Ser Phe Gly Thr Thr Ser Arg Ala Gly Thr Ala Asp Leu Phe Gln Ile Ala Asn Ser Glu Glu Gln Tyr Arg Gln Ala Leu Leu Asp Tyr Ser Gly Gly Asp Lys Thr Asp Glu Gly Ile Arg Leu Met Gln Gln Ser Asp Tyr Gly Asn Leu Ser Tyr His Ile Arg Asn Lys Asn Met Leu Phe Ile Phe Ser Thr Gly Asn Asp Ala Gln Ala Gln Pro Asn Thr Tyr Ala Leu Leu Pro Phe Tyr Glu Lys Asp Ala Gln Lys Gly Ile Ile Thr Val Ala Gly Val Asp 325 330 :335 Arg Ser Gly Glu Lys Phe Lys Arg Glu Met Tyr Gly Glu Pro Gly Thr Glu Pro Leu Glu Tyr Gly Ser Asn His Cys Gly Ile Thr Ala Met Trp Cys Leu Ser Ala Pro Tyr Glu Ala Ser Val Arg Phe Thr Arg Thr Asn Pro Ile Gln Ile Ala Gly Thr Ser Phe Ser Ala Pro Ile Val Thr Gly Thr Ala Ala Leu Leu Leu Gln Lys Tyr Pro Trp Met Ser Asn Asp Asn Leu Arg Thr Thr Leu Leu Thr Thr Ala Gln Asp Ile Gly Ala Val Gly Val Asp Ser Lys Phe Gly Trp Gly Leu Leu Asp Ala Gly Lys Ala Met Asn Gly Pro Ala Ser Phe Pro Phe Gly Asp Phe Thr Ala Asp Thr Lys Gly Thr Ser Asp Ile Ala Tyr Ser Phe Arg Asn Asp Ile Ser Gly Thr Gly Gly Leu Ile Lys Lys Gly Gly Ser Gln Leu Gln Leu His Gly Asn Asn Thr Tyr Thr Gly Lys Thr Ile Ile Glu Gly Gly Ser Leu Val Leu Tyr Gly Asn Asn Lys Ser Asp Met Arg Val Glu Thr Lys Gly Ala Leu Ile Tyr Asn Gly Ala Ala Ser Gly Gly Ser Leu Asn Ser Asp Gly Ile Val Tyr Leu Ala Asp Thr Asp Gln Ser Gly Ala Asn Glu Thr Val His Ile Lys Gly Ser Leu Gln Leu Asp Gly Lys Gly Thr Leu Tyr Thr Arg Leu Gly Lys Leu Leu Lys Val Asp Gly Thr Ala Ile Ile Gly Gly Lys Leu Tyr Met Ser Ala Arg Gly Lys Gly Ala Gly Tyr Leu Asn Ser Thr Gly Arg Arg Val Pro Phe Leu Ser Ala Ala Lys Ile Gly Gln Asp Tyr Ser Phe Phe Thr Asn Ile Glu Thr Asp Gly Gly Leu Leu Ala Ser Leu Asp Ser Val Glu Lys Thr Ala Gly Ser Glu Gly Asp Thr Leu Ser Tyr Tyr Val Arg Arg Gly Asn Ala Ala Arg Thr Ala Ser Ala Ala Ala His Ser Ala Pro Ala Gly Leu Lys His Ala Val Glu Gln Gly Gly Ser Asn Leu Glu Asn Leu Met Val Glu Leu Asp Ala Ser Glu Ser Ser Ala Thr Pro Glu Thr Val Glu Thr Ala Ala Ala Asp Arg Thr Asp Met Pro Gly Ile Arg Pro Tyr Gly Ala Thr Phe Arg Ala Ala Ala Ala Val Gln His Ala Asn Ala Ala Asp Gly Val Arg Ile Phe Asn Ser Leu Ala Ala Thr Val Tyr Ala Asp Ser Thr Ala Ala His Ala Asp Met Gin Gly Arg Arg Leu Lys Ala Val Ser Asp Gly Leu Asp His Asn Gly Thr Gly Leu Arg Val Ile Ala Gln Thr Gin Gin Asp Gly Gly Thr Trp Glu Gin Gly Gly Val Glu Gly Lys Met Arg Gly Ser Thr Gin Thr Val Gly Ile Ala Ala Lys Thr Gly Glu Asn Thr Thr Ala Ala Ala Thr Leu Gly Met Gly Arg Ser Thr Trp Ser Glu Asn Ser Ala Asn Ala Lys Thr Asp Ser Ile Ser Leu Phe Ala Gly Ile Arg His Asp Ala Gly Asp Ile Gly Tyr Leu Lys Gly Leu Phe Ser Tyr Gly Arg Tyr Lys Asn Ser Ile Ser Arg Ser Thr Gly Ala Asp Glu His Ala Glu Gly Ser Val Asn Gly Thr Leu Met Gin Leu Gly Ala Leu Gly Gly Val Asn Val Pro Phe Ala Ala Thr Gly Asp Leu Thr Val Glu Gly Gly Leu Arg Tyr Asp Leu Leu Lys Gln Asp Ala Phe Ala Glu Lys Gly Ser Ala Leu Gly Trp Ser Gly Asn Ser Leu Thr Glu Gly Thr Leu Val Gly Leu Ala Gly Leu Lys Leu Ser Gln Pro Leu Ser Asp Lys Ala Val Leu Phe Ala Thr Ala Gly Val Glu Arg Asp Leu Asn Gly Arg Asp Tyr Thr Val Thr Gly Gly Phe Thr Gly Ala Thr Ala Ala Thr Gly Lys Thr Gly Ala Arg Asn Met Pro His Thr Arg Leu Val Ala Gly Leu Gly Ala Asp Val Glu Phe Gly Asn Gly Trp Asn Gly Leu Ala Arg Tyr Ser Tyr Ala Gly Ser Lys Gin Tyr Gly Asn His Ser Gly Arg Val Gly Val Gly Tyr Arg Phe Leu Glu Gly Ser Gly Gly Gly Gly Val Ala Ala Asp Ile Gly Ala Gly Leu Ala Asp Ala Leu Thr Ala Pro Leu Asp His Lys Asp Lys Gly Leu Gln Ser Leu Thr Leu Asp Gln Ser Val Arg Lys Asn Glu Lys Leu Lys Leu Ala Ala Gln Gly Ala Glu Lys Thr Tyr Gly Asn Gly Asp Ser Leu Asn Thr Gly Lys Leu Lys Asn Asp Lys Val Ser Arg Phe Asp Phe Ile Arg Gin Ile Glu Val Asp Gly Gin 1125 1130 1.135 Leu Ile Thr Leu Glu Ser Gly Glu Phe Gln Val Tyr Lys Gln Ser His Ser Ala Leu Thr Ala Phe Gln Thr Glu Gln Ile Gln Asp Ser Glu His Ser Gly Lys Met Val Ala Lys Arg Gln Phe Arg Ile Gly Asp Ile Ala Gly Glu His Thr Ser Phe Asp Lys Leu Pro Glu Gly Gly Arg Ala Thr 1185 1190 1.195 1200 Tyr Arg Gly Thr Ala Phe Gly Ser Asp Asp Ala Gly Gly Lys Leu Thr Tyr Thr Ile Asp Phe Ala Ala Lys Gln Gly Asn Gly Lys Ile Glu His Leu Lys Ser Pro Glu Leu Asn Val Asp Leu Ala Ala Ala Asp :Ile Lys Pro Asp Gly Lys Arg His Ala Val Ile Ser Gly Ser Val Leu Tyr Asn Gln Ala Glu Lys Gly Ser Tyr Ser Leu Gly Ile Phe Gly Gly Lys Ala Gln Glu Val Ala Gly Ser Ala Glu Val Lys Thr Val Asn Gly Ile Arg His Ile Gly Leu Ala Ala Lys Gln Leu Glu His His His His His His <210> 21 <211> 4344 <212> DNA
<213> Artificial Sequence <220>
<223> deltaG983-961 <400> 21 atgacttctg cgcccgactt caatgcaggc ggtaccggta tcggcagcaa cagcagagca 60 acaacagcga aatcagcagc agtatcttac gccggtatca agaacgaaat gtgcaaagac 120 agaagcatgc tctgtgccgg tcgggatgac gttgcggtta cagacaggga tgccaaaatc 180 aatgcccccc ccccgaatct gcataccgga gactttccaa acccaaatga cgcatacaag 240 aatttgatca acctcaaacc tgcaattgaa gcaggctata caggacgcgg ggtagaggta 300 ggtatcgtcg acacaggcga atccgtcggc agcatatcct ttcccgaact gtatggcaga 360 aaagaacacg gctataacga aaattacaaa aactatacgg cgtatatgcg gaaggaagcg 420 cctgaagacg gaggcggtaa agacattgaa gcttctttcg acgatgaggc cgttatagag 480 actgaagcaa agccgacgga tatccgccac gtaaaagaaa tcggacacat cgatttggtc 540 tcccatatta ttggcgggcg ttccgtggac ggcagacctg caggcggtat tgcgcccgat 600 gcgacgctac acataatgaa tacgaatgat gaaaccaaga acgaaatgat ggttgcagcc 660 atccgcaatg catgggtcaa gctgggcgaa cgtggcgtgc gcatcgtcaa taacagtttt 720 ggaacaacat cgagggcagg cactgccgac cttttccaaa tagccaattc ggaggagcag 780 taccgccaag cgttgctcga ctattccggc ggtgataaaa cagacgaggg tatccgcctg 840 atgcaacaga gcgattacgg caacctgtcc taccacatcc gtaataaaaa catgcttttc 900 atcttttcga caggcaatga cgcacaagct cagcccaaca catatgccct at:tgccattt 960 tatgaaaaag acgctcaaaa aggcattatc acagtcgcag gcgtagaccg cagtggagaa 1020 aagttcaaac gggaaatgta tggagaaccg ggtacagaac cgcttgagta tggctccaac 1080 cattgcggaa ttactgccat gtggtgcctg tcggcaccct atgaagcaag cgtccgtttc 1140 acccgtacaa acccgattca aattgccgga acatcctttt ccgcacccat cgtaaccggc 1200 acggcggctc tgctgctgca gaaatacccg tggatgagca acgacaacct gcgtaccacg 1260 ttgctgacga cggctcagga catcggtgca gtcggcgtgg acagcaagtt cggctgggga 1320 ctgctggatg cgggtaaggc catgaacgga cccgcgtcct ttccgttcgg cgactttacc 1380 gccgatacga aaggtacatc cgatattgcc tactccttcc gtaacgacat ttcaggcacg 1440 ggcggcctga tcaaaaaagg cggcagccaa ctgcaactgc acggcaacaa cacctatacg 1500 ggcaaaacca ttatcgaagg cggttcgctg gtgttgtacg gcaacaacaa atcggatatg 1560 cgcgtcgaaa ccaaaggtgc gctgatttat aacggggcgg catccggcgg cagcctgaac 1620 agcgacggca ttgtctatct ggcagatacc gaccaatccg gcgcaaacga aaccgtacac 1680 atcaaaggca gtctgcagct ggacggcaaa ggtacgctgt acacacgttt gggcaaactg 1740 ctgaaagtgg acggtacggc gattatcggc ggcaagctgt acatgtcggc acgcggcaag 1800 ggggcaggct atctcaacag taccggacga cgtgttccct tcctgagtgc cgccaaaatc 1860 gggcaggatt attctttctt cacaaacatc gaaaccaagg gcggcctgct ggcttccctc 1920 gacagcgtcg aaaaaacagc gggcagtgaa ggcgacacgc tgtcctatta tgtccgtcgc 1980 ggcaatgcgg cacggactgc ttcggcagcg gcacattccg cgcccgccgg tctgaaacac 2040 gccgtagaac agggcggcag caatctggaa aacctgatgg tcgaactgga tgcctccgaa 2100 tcatccgcaa cacccgagac ggttgaaact gcggcagccg accgcacaga tatgccgggc 2160 atccgcccct acggcgcaac tttccgcgca gcggcagccg tacagcatgc gaatgccgcc 2220 gacggtgtac gcatcttcaa cagtctcgcc gctaccgtct atgccgacag taccgccgcc 2280 catgccgata tgcaggcacg ccgcctgaaa gccgtatcgg acgggttgga ccacaacggc 2340 acgggtctgc gcgtcatcgc gcaaacccaa caggacggtg gaacgtggga acagggcggt 2400 gttgaaggca aaatgcgcgg cagtacccaa accgtcggca ttgccgcgaa aaccggcgaa 2460 aatacgacag cagccgccac actgggcatg ggacgcagca catggagcga aaacagtgca 2520 aatgcaaaaa ccgacagcat tagtctgttt gcaggcatac ggcacgatgc gggcgatatc 2580 ggctatctca aaggcctgtt ctcctacgga cgctacaaaa acagcatcag ccgcagcacc 2640 ggtgcggacg aacatgcgga aggcagcgtc aacggcacgc tgatgcagct gggcgcactg 2700 ggcggtgtca acgttccgtt tgccgcaacg ggagatttga cggtcgaagg cggtttgcgc 2760 tacgacctgc tcaaacagga tgcattcgcc gaaaaaggca gtgctttggg ctggagcggc 2820 aacagcctca ctgaaggcac gctggtcgga cttccgggtc tgaagctgtc gcaacccttg 2880 agcgataaag ccgtcctgtt tgcaacggcg ggcgtggaac gcgacctgaa cggacgcgac 2940 tacacggtaa cgggcggctt taccggcgcg actgcagcaa ccggcaagac gggggcacgc 3000 aatatgccgc acacccgtct ggttgccggc ctgggcgcgg atgtcgaatt cggcaacggc 3060 tggaacggct tggcacgtta cagctacgcc ggttccaaac agtacggcaa ccacagcgga 3120 cgagtcggcg taggctaccg gttcctcgag ggtggcggag gcactggatc cgccaca.aac 3180 gacgacgatg ttaaaaaagc tgccactgtg gccattgctg ctgcctacaa caatggccaa 3240 gaaatcaacg gtttcaaagc tggagagacc atctacgaca ttgatgaaga cggcacaatt 3300 accaaaaaag acgcaactgc agccgatgtt gaagccgacg actttaaagg tctgggtctg 3360 aaaaaagtcg tgactaacct gaccaaaacc gtcaatgaaa acaaacaaaa cgtcgatgcc 3420 aaagtaaaag ctgcagaatc tgaaatagaa aagttaacaa ccaagttagc agacactgat 3480 gccgctttag cagatactga tgccgctgtg gatgcaacca ccaacgcctt gaataaattg 3540 ggagaaaata taacgacatt tgctgaagag actaagacaa atatcgtaaa aattgatgaa 3600 aaattagaag ccgtggctga taccgtcgac aagcatgccg aagcattcaa cgatatcgcc 3660 gattcattgg atgaaaccaa cactaaggca gacgaagccg tcaaaaccgc caatgaagcc 3720 aaacagacgg ccgaagaaac caaacaaaac gtcgatgcca aagtaaaagc tgcagaaact 3780 gcagcaggca aagccgaagc tgccgctggc acagctaata ctgcagccga caaggccgaa 3840 gctgtcgctg caaaagttac cgacatcaaa gctgatatcg ctacgaacaa agataatatt 3900 gctaaaaaag caaacagtgc cgacgtgtac accagagaag agtctgacag caaatttgtc 3960 agaattgatg gtctgaacgc tactaccgaa aaattggaca cacgcttggc ttctgctgaa 4020 aaatccattg ccgatcacga tactcgcctg aacggtttgg ataaaacagt gtcagacctg 4080 cgcaaagaaa cccgccaagg ccttgcagaa caagccgcgc tctccggtct gttccaacct 4140 tacaacgtgg gtcggttcaa tgtaacggct gcagtcggcg gctacaaatc cgaatcggca 4200 gtcgccatcg gtaccggctt ccgctttacc gaaaactttg ccgccaaagc aggcgtggca 4260 gtcggcactt cgtccggttc ttccgcagcc taccatgtcg gcgtcaatta cgagtggctc 4320 gagcaccacc accaccacca ctga 4344 <210> 22 <211> 1447 <212> PRT
<213> Artificial sequence <220>
<223> deltaG983-961 <400> 22 Met Thr Ser Ala Pro Asp Phe Asn Ala Gly Gly Thr Gly Ile Gly Ser Asn Ser Arg Ala Thr Thr Ala Lys Ser Ala Ala Val Ser Tyr Ala Gly Ile Lys Asn Glu Met Cys Lys Asp Arg Ser Met Leu Cys Ala Gly Arg Asp Asp Val Ala Val Thr Asp Arg Asp Ala Lys Ile Asn Ala Pro Pro Pro Asn Leu His Thr Gly Asp Phe Pro Asn Pro Asn Asp Ala Tyr Lys Asn Leu Ile Asn Leu Lys Pro Ala Ile Glu Ala Gly Tyr Thr Gly Arg Gly Val Glu Val Gly Ile Val Asp Thr Gly Glu Ser Val Gly Ser Ile Ser Phe Pro Glu Leu Tyr Gly Arg Lys Glu His Gly Tyr Asn Glu Asn Tyr Lys Asn Tyr Thr Ala Tyr Met Arg Lys Glu Ala Pro Glu Asp Gly Gly Gly Lys Asp Ile Glu Ala Ser Phe Asp Asp Glu Ala Val Ile Glu Thr Glu Ala Lys Pro Thr Asp Ile Arg His Val Lys Glu Ile Gly His Ile Asp Leu Val Ser His Ile Ile Gly Gly Arg Ser Val Asp Gly Arg Pro Ala Gly Gly Ile Ala Pro Asp Ala Thr Leu His Ile Met Asn Thr Asn Asp Glu Thr Lys Asn Glu Met Met Val Ala Ala Ile Arg Asn Ala Trp Val Lys Leu Gly Glu Arg Gly Val Arg Ile Val Asn Asn Ser Phe Gly Thr Thr Ser Arg Ala Gly Thr Ala Asp Leu Phe Gln Ile Ala Asn Ser Glu Glu Gln Tyr Arg Gln Ala Leu Leu Asp Tyr Ser Gly Gly Asp Lys Thr Asp Glu Gly Ile Arg Leu Met Gln Gln Ser Asp Tyr Gly Asn Leu Ser Tyr His Ile Arg Asn Lys Asn Met Leu Phe Ile Phe Ser Thr Gly Asn Asp Ala Gln Ala Gln Pro Asn Thr Tyr Ala Leu Leu Pro Phe Tyr Glu Lys Asp Ala Gln Lys Gly Ile Ile Thr Val Ala Gly Val Asp Arg Ser Gly Glu Lys Phe Lys Arg Glu Met Tyr Gly Glu Pro Gly Thr Glu Pro Leu Glu Tyr Gly Ser Asn His Cys Gly Ile Thr Ala Met Trp Cys Leu Ser Ala Pro Tyr Glu Ala Ser Val Arg Phe Thr Arg Thr Asn Pro Ile Gln Ile Ala Gly Thr Ser Phe Ser Ala Pro Ile Val Thr Gly Thr Ala Ala Leu Leu Leu Gln Lys Tyr Pro Trp Met Ser Asn Asp Asn Leu Arg Thr Thr Leu Leu Thr Thr Ala Gln Asp Ile Gly Ala Val Gly Val Asp Ser Lys Phe Gly Trp Gly Leu Leu Asp Ala Gly Lys Ala Met Asn Gly Pro Ala Ser Phe Pro Phe Gly Asp Phe Thr Ala Asp Thr Lys Gly Thr Ser Asp Ile Ala Tyr Ser Phe Arg Asn Asp Ile Ser Gly Thr Gly Gly Leu Ile Lys Lys Gly Gly Ser Gln Leu Gln Leu His Gly Asn Asn Thr Tyr Thr Gly Lys Thr Ile Ile Glu Gly Gly Ser Leu Val Leu Tyr Gly Asn Asn Lys Ser Asp Met Arg Val Giu Thr Lys Gly Ala Leu Ile Tyr Asn Gly Ala Ala Ser Gly Gly Ser Leu Asn Ser Asp Gly Ile Val Tyr Leu Ala Asp Thr Asp Gln Ser Gly Ala Asn Glu Thr Val His Ile Lys Gly Ser Leu Gln Leu Asp Gly Lys Gly Thr Leu Tyr Thr Arg Leu Gly Lys Leu Leu Lys Val Asp Gly Thr Ala Ile Ile Gly Gly Lys Leu Tyr Met Ser Ala Arg Gly Lys Gly Ala Gly Tyr Leu Asn Ser Thr Gly Arg Arg Val Pro Phe Leu Ser Ala Ala Lys Ile Gly Gln Asp Tyr Ser Phe Phe Thr Asn Ile Glu Thr Asp Gly Gly Leu Leu Ala Ser Leu Asp Ser Val Glu Lys Thr Ala Gly Ser Glu Gly Asp Thr Leu Ser Tyr Tyr Val Arg Arg Gly Asn Ala Ala Arg Thr Ala Ser Ala Ala Ala His Ser Ala Pro Ala Gly Leu Lys His Ala Val Glu Gin Gly Gly Ser Asn Leu Glu Asn Leu Met Val Glu Leu Asp Ala Ser Glu Ser Ser Ala Thr Pro Glu Thr Val Glu Thr Ala Ala Ala Asp Arg Thr Asp Met Pro Gly Ile Arg Pro Tyr Gly Ala Thr Phe Arg Ala Ala Ala Ala Val Gin His Ala Asn Ala Ala Asp Gly Val Arg Ile Phe Asn Ser Leu Ala Ala Thr Val Tyr Ala Asp Ser Thr Ala Ala His Ala Asp Met Gin Gly Arg Arg Leu Lys Ala Val Ser Asp Gly Leu Asp His Asn Gly Thr Gly Leu Arg Val Ile Ala Gin Thr Gln Gln Asp Gly Gly Thr Trp Glu Gln Gly Gly Val Glu Gly Lys Met Arg Gly Ser Thr Gln Thr Val Gly Ile Ala Ala Lys Thr Gly Glu Asn Thr Thr Ala Ala Ala Thr Leu Gly Met Gly Arg Ser Thr Trp Ser Glu Asn Ser Ala Asn Ala Lys Thr Asp Ser Ile Ser Leu Phe Ala Gly Ile Arg His Asp Ala Gly Asp Ile Gly Tyr Leu Lys Gly Leu Phe Ser Tyr Gly Arg Tyr Lys Asn Ser Ile Ser Arg Ser Thr Gly Ala Asp Glu His Ala Glu Gly Ser Val Asn Gly Thr Leu Met Gln Leu Gly Ala Leu Gly Gly Val Asn Val Pro Phe Ala Ala Thr Gly Asp Leu Thr Val Glu Gly Gly Leu Arg Tyr Asp Leu Leu Lys Gin Asp Ala Phe Ala Glu Lys Gly Ser Ala Leu Gly Trp Ser Gly Asn Ser Leu Thr Glu Gly Thr Leu Val Gly Leu Ala Gly Leu Lys Leu Ser Gln Pro Leu Ser Asp Lys Ala Val Leu Phe Ala Thr Ala Gly Val Glu Arg Asp Leu Asn Gly Arg Asp Tyr Thr Val Thr Gly Gly Phe Thr Gly Ala Thr Ala Ala Thr Gly Lys Thr Gly Ala Arg Asn Net Pro His Thr Arg Leu Val Ala Gly Leu Gly Ala Asp Val Glu Phe Gly Asn Gly Trp Asn Gly Leu Ala Arg Tyr Ser Tyr Ala Gly Ser Lys Gln Tyr Gly Asn His Ser Gly Arg Val Gly Val Gly Tyr Arg Phe Leu Glu Gly Gly Gly Gly Thr Gly Ser Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile Ala Ala Ala Tyr Asn Asn Gly Gln Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gln Thr Ala Glu Glu Thr Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val. Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gln Gly Leu Ala Glu Gln Ala Ala Leu Ser Gly Leu Phe Gln Pro Tyr Asn Val Gly Arg Phe Asn Val Thr Ala Ala Val Gly Gly Tyr Lys Ser Glu Ser Ala Val Ala Ile Gly Thr Gly Phe Arg Phe Thr Glu Asn Phe Ala Ala Lys Ala Gly Val Ala Val Gly Thr Ser Ser Gly Ser Ser Ala Ala Tyr His Val Gly Val Asn Tyr Glu Trp Leu Glu His His His His His His <210> 23 <211> 4179 <212> DNA
<213> Artificial Sequence <220>
<223> deltaG983-961c <400> 23 atgacttctg cgcccgactt caatgcaggc ggtaccggta tcggcagcaa cagcagagca 60 acaacagcga aatcagcagc agtatcttac gccggtatca agaacgaaat gtgcaaagac 120 agaagcatgc tctgtgccgg tcgggatgac gttgcggtta cagacaggga tgccaaaatc 180 aatgcccccc ccccgaatct gcataccgga gactttccaa acccaaatga cgcatacaag 240 aatttgatca acctcaaacc tgcaattgaa gcaggctata caggacgcgg ggtacaggta 300 ggtatcgtcg acacaggcga atccgtcggc agcatatcct ttcccgaact gtatggcaga 360 aaagaacacg gctataacga aaattacaaa aactatacgg cgtatatgcg gaaggaagcg 420 cctgaagacg gaggcggtaa agacattgaa gcttctttcg acgatgaggc cgttatagag 480 actgaagcaa agccgacgga tatccgccac gtaaaagaaa tcggacacat cgatttggtc 540 tcccatatta ttggcgggcg ttccgtggac ggcagacctg caggcggtat tgcgcccgat 600 gcgacgctac acataatgaa tacgaatgat gaaaccaaga acgaaatgat gtttgcagcc 660 atccgcaatg catgggtcaa gctgggcgaa cgtggcgtgc gcatcgtcaa taacagtttt 720 ggaacaacat cgagggcagg cactgccgac cttttccaaa tagccaattc ggaggagcag 780 taccgccaag cgttgctcga ctattccggc ggtgataaaa cagacgaggg tatccgcctg 840 atgcaacaga gcgattacgg caacctgtcc taccacatcc gtaataaaaa catgcttttc 900 atcttttcga caggcaatga cgcacaagct cagcccaaca catatgccct attgccattt 960 tatgaaaaag acgctcaaaa aggcattatc acagtcgcag gcgtagaccg cagtggagaa 1020 aagttcaaac gggaaatgta tggagaaccg ggtacagaac cgcttgagta tggctccaac 1080 cattgcggaa ttactgccat gtggtgcctg tcggcaccct atgaagcaag cgtccgtttc 1140 acccgtacaa acccgattca aattgccgga acatcctttt ccgcacccat cgtaaccggc 1200 acggcggctc tgctgctgca gaaatacccg tggatgagca acgacaacct gcgtaccacg 1260 ttgctgacga cggctcagga catcggtgca gtcggcgtgg acagcaagtt cggctgggga 1320 ctgctggatg cgggtaaggc catgaacgga cccgcgtcct ttccgttcgg cgactttacc 1380 gccgatacga aaggtacatc cgatattgcc tactccttcc gtaacgacat ttcaggcacg 1440 ggcggcctga tcaaaaaagg cggcagccaa ctgcaactgc acggcaacaa cacctatacg 1500 ggcaaaacca ttatcgaagg cggttcgctg gtgttgtacg gcaacaacaa atcggatatg 1560 cgcgtcgaaa ccaaaggtgc gctgatttat aacggggcgg catccggcgg cagcctgaac 1620 agcgacggca ttgtctatct ggcagatacc gaccaatccg gcgcaaacga aaccgtacac 1680 atcaaaggca gtctgcagct ggacggcaaa ggtacgctgt acacacgttt gggcaaactg 1740 ctgaaagtgg acggtacggc gattatcggc ggcaagctgt acatttcggc acgcggcaag 1800 ggggcaggct atctcaacag taccggacga cgtgttccct tcctgagtgc cgccaaaatc 1860 gggcaggatt attctttctt cacaaacatc gaaaccgacg gcggcctgct ggcttccctc 1920 gacagcgtcg aaaaaacagc gggcagtgaa ggcgacacgc tgtcctatta tgtccgtcgc 1980 ggcaatgcgg cacggactgc ttcggcagcg gcacattccg cgcccgccgg tctgaaacac 2040 gccgtagaac agggcggcag caatctggaa aacctgatgg tcgaactgga tgcctccgaa 2100 tcatccgcaa cacccgagac ggttgaaact gcggcagccg accgcacaga tatgccgggc 2160 atccgcccct acggcgcaac tttccgccca gcggcagccg tacagcatgc gaatgccgcc 2220 gacggtgtac gcatcttcaa cagtctcgcc gctaccgtct atgccgacag taccgccgcc 2280 catgccgata tgcagggacg ccgcctgaaa gccgtatcgg acgggttgga ccacaacggc 2340 acgggtctgc gcgtcatcgc gcaaacccaa caggacggtg gaacgtggga acagggcggt 2400 gttgaaggca aaatgcgcgg cagtacccaa accgtcggca ttgccgcgaa aaccggcgaa 2460 aatacgacag cagccgccac actgggcatg ggacgcagca catggagcga aaacagtgca 2520 aatgcaaaaa ccgacagcat tagtctgttt gcaggcatac ggcacgatgc gggcgatatc 2580 ggctatctca aaggcctgtt ctcctacgga cgctacaaaa acagcatcag ccgcagcacc 2640 ggtgcggacg aacatgcgga aggcagcgtc aacggcacgc tgatgcagct gggcgcactg 2700 ggcggtgtca acgttccgtt tgccgcaacg ggagatttga cggtcgaagg cggtctgcgc 2760 tacgacctgc tcaaacagga tgcattcgcc gaaaaaggca gtgctttggg ctggagcggc 2820 aacagcctca ctgaaggcac gctggtcgga ctcgcgggtc tgaagctgtc gcaacccttg 2880 agcgataaag ccgtcctgtt tgcaacggcg ggcgtggaac gcgacctgaa cggacgcgac 2940 tacacggtaa cgggcggctt taccggcgcg actgcagcaa ccggcaagac gggggcacgc 3000 aatatgcccc acacccgtct ggttgccggc ctgggcgcgg atgtcgaatt cggcaacggc 3060 tggaacggct tggcacgtta cagcttcgcc ggttccaaac agtacggcaa ccacagcgga 3120 cgagtcggcg taggctaccg gttcctcgag ggtggcggag gcactggatc cgccacaaac 3180 gacgacgatg ttaaaaaagc tgccactgtg gccattgctg ctgcctacaa caatggccaa 3240 gaaatcaacg gtttcaaagc tggagagacc atctacgaca ttgatgaaga cggcacaatt 3300 accaaaaaag acgcaactgc agccgatgtt gaagccgacg actttaaagg tctgggtctg 3360 aaaaaagtcg tgactaacct gaccaaaacc gtcaatgaaa acaaacaaaa cgtcgatgcc 3420 aaagtaaaag ctgcagaatc tgaaatagaa aagttaacaa ccaagttagc agacactgat 3480 gccgctttag cagatactga tgccgctctg gatgcaacca ccaacgcctt gaataaattg 3540 ggagaaaata taacgacatt tgctgaagag actaagacaa atatcgtaaa aattgatgaa 3600 aaattagaag ccgtggctga taccgtcgac aagcatgccg aagcattcaa cgatatcgcc 3660 gattcattgg atgaaaccaa cactaaggca gacgaagccg tcaaaaccgc caatgaagcc 3720 aaacagacgg ccgaagaaac caaacaaaac gtcgatgcca aagtaaaagc tgcagaaact 3780 gcagcaggca aagccgaagc tgccgctggc acagctaata ctgcagccga caaggccgaa 3840 gctgtcgctg caaaagttac cgacatcaaa gctgatatcg ctacgaacaa agataatatt 3900 gctaaaaaag caaacagtgc cgacgtgtac accagagaag agtctgacag caaatttgtc 3960 agaattgatg gtctgaacgc tactaccgaa aaattggaca cacgcttggc ttctgctgaa 4020 aaatccattg ccgatcacga tactcgcctg aacggtttgg ataaaacagt gtcagacctg 4080 cgcaaagaaa cccgccaagg ccttgcagaa caagccgcgc tctccggtct gttccaacct 4140 tacaacgtgg gtctcgagca ccaccaccac caccactga 4179 <210> 24 <211> 1392 <212> PRT
<213> Artificial Sequence <220>
<223> deltaG983-961c <400> 24 Met Thr Ser Ala Pro Asp Phe Asn Ala Gly Gly Thr Gly Ile Gly Ser Asn Ser Arg Ala Thr Thr Ala Lys Ser Ala Ala Val Ser Tyr Ala Gly Ile Lys Asn Glu Met Cys Lys Asp Arg Ser Met Leu Cys Ala Gly Arg Asp Asp Val Ala Val Thr Asp Arg Asp Ala Lys Ile Asn Ala Pro Pro Pro Asn Leu His Thr Gly Asp Phe Pro Asn Pro Asn Asp Ala Tyr Lys Asn Leu Ile Asn Leu Lys Pro Ala Ile Glu Ala Gly Tyr Thr Gly Arg Gly Val Glu Val Gly Ile Val Asp Thr Gly Glu Ser Val Gly Ser Ile Ser Phe Pro Glu Leu Tyr Gly Arg Lys Glu His Gly Tyr Asn Glu Asn Tyr Lys Asn Tyr Thr Ala Tyr Met Arg Lys Glu Ala Pro Glu Asp Gly Gly Gly Lys Asp Ile Glu Ala Ser Phe Asp Asp Glu Ala Val Ile Glu Thr Glu Ala Lys Pro Thr Asp Ile Arg His Val Lys Glu Ile Gly His Ile Asp Leu Val Ser His Ile Ile Gly Gly Arg Ser Val Asp Gly Arg Pro Ala Gly Gly Ile Ala Pro Asp Ala Thr Leu His Ile Met Asn Thr Asn Asp Glu Thr Lys Asn Glu Met Met Val Ala Ala Ile Arg Asn Ala Trp Val Lys Leu Gly Glu Arg Gly Val Arg Ile Val Asn Asn Ser Phe Gly Thr Thr Ser Arg Ala Gly Thr Ala Asp Leu Phe Gln Ile Ala Asn Ser Glu Glu Gln Tyr Arg Gln Ala Leu Leu Asp Tyr Ser Gly Gly Asp Lys Thr Asp Glu Gly Ile Arg Leu Met Gln Gln Ser Asp Tyr Gly Asn Leu Ser Tyr His Ile Arg Asn Lys Asn Met Leu Phe Ile Phe Ser Thr Gly Asn Asp Ala Gln Ala Gln Pro Asn Thr Tyr Ala Leu Leu Pro Phe Tyr Glu Lys Asp Ala Gln Lys Gly Ile Ile Thr Val Ala Gly Val Asp Arg Ser Gly Glu Lys Phe Lys Arg Glu Met Tyr Gly Glu Pro Gly Thr Glu Pro Leu Glu Tyr Gly Ser Asn His Cys Gly Ile Thr Ala Met Trp Cys Leu Ser Ala Pro Tyr Glu Ala Ser Val Arg Phe Thr Arg Thr Asn Pro Ile Gln Ile Ala Gly Thr Ser Phe Ser Ala Pro Ile Val Thr Gly Thr Ala Ala Leu Leu Leu Gln Lys Tyr Pro Trp Met Ser Asn Asp Asn Leu Arg Thr Thr Leu Leu Thr Thr Ala Gln Asp Ile Gly Ala Val Gly Val Asp Ser Lys Phe Gly Trp Gly Leu Leu Asp Ala Gly Lys Ala Met Asn Gly Pro Ala Ser Phe Pro Phe Gly Asp Phe Thr Ala Asp Thr Lys Gly Thr Ser Asp Ile Ala Tyr Ser Phe Arg Asn Asp Ile Ser Gly Thr Gly Gly Leu Ile Lys Lys Gly Gly Ser Gln Leu Gln Leu His Gly Asn Asn Thr Tyr Thr Gly Lys Thr Ile Ile Glu Gly Gly Ser Leu Val Leu Tyr Gly Asn Asn Lys Ser Asp Met Arg Val Glu Thr Lys Gly Ala Leu Ile Tyr Asn Gly Ala Ala Ser Gly Gly Ser Leu Asn Ser Asp Gly Ile Val Tyr Leu Ala Asp Thr Asp Gln Ser Gly Ala Asn Glu Thr Val His Ile Lys Gly Ser Leu Gln Leu Asp Gly Lys Gly Thr Leu Tyr Thr Arg Leu Gly Lys Leu Leu Lys Val Asp Gly Thr Ala Ile Ile Gly Gly Lys Leu Tyr Met Ser Ala Arg Gly Lys Gly Ala Gly Tyr Leu Asn Ser Thr Gly Arg Arg Val Pro Phe Leu Ser Ala Ala Lys Ile Gly Gln Asp Tyr Ser Phe Phe Thr Asn Ile Glu Thr Asp Gly Gly Leu Leu Ala Ser Leu Asp Ser Val Glu Lys Thr Ala Gly Ser Glu Gly Asp Thr Leu Ser Tyr Tyr Val Arg Arg Gly Asn Ala Ala Arg Thr Ala Ser Ala Ala Ala His Ser Ala Pro Ala Gly Leu Lys His Ala Val Glu Gln Gly Gly Ser Asn Leu Glu Asn Leu Met Val Glu Leu Asp Ala Ser Glu Ser Ser Ala Thr Pro Glu Thr Val Glu Thr Ala Ala Ala Asp Arg Thr Asp Met Pro Gly Ile Arg Pro Tyr Gly Ala Thr Phe Arg Ala Ala Ala Ala Val Gln His Ala Asn Ala Ala Asp Gly Val Arg Ile Phe Asn Ser Leu Ala Ala Thr Val Tyr Ala Asp Ser Thr Ala Ala His Ala Asp Met Gln Gly Arg Arg Leu Lys Ala Val Ser Asp Gly Leu Asp His Asn Gly Thr Gly Leu Arg Val Ile Ala Gln Thr Gln Gln Asp Gly Gly Thr Trp Glu Gln Gly Gly Val Glu Gly Lys Met Arg Gly Ser Thr Gln Thr Val Gly Ile Ala Ala Lys Thr Gly Glu Asn Thr Thr Ala Ala Ala Thr Leu Gly Met Gly Arg Ser Thr Trp Ser Glu Asn Ser Ala Asn Ala Lys Thr Asp Ser Ile Ser Leu Phe Ala Gly Ile Arg His Asp Ala Gly Asp Ile Gly Tyr Leu Lys Gly Leu Phe Ser Tyr Gly Arg Tyr Lys Asn Ser Ile Ser Arg Ser Thr Gly Ala Asp Glu His Ala Glu Gly Ser Val Asn Gly Thr Leu Met Gln Leu Gly Ala Leu Gly Gly Val Asn Val Pro Phe Ala Ala Thr Gly Asp Leu Thr Val Glu Gly Gly Leu Arg Tyr Asp Leu Leu Lys Gln Asp Ala Phe Ala Glu Lys Gly Ser Ala Leu Gly Trp Ser Gly Asn Ser Leu Thr Glu Gly Thr Leu Val Gly Leu Ala Gly Leu Lys Leu Ser Gin Pro Leu Ser Asp Lys Ala Val Leu Phe Ala Thr Ala Gly Val Glu Arg Asp Leu Asn Gly Arg Asp Tyr Thr Val Thr Gly Gly Phe Thr Gly Ala Thr Ala Ala Thr Gly Lys Thr Gly Ala Arg Asn Met Pro His Thr Arg Leu Val Ala Gly Leu Gly Ala Asp Val Glu Phe Gly Asn Gly Trp Asn Gly Leu Ala Arg Tyr Ser Tyr Ala Gly Ser Lys Gln Tyr Gly Asn His Ser Gly Arg Val Gly Val Gly Tyr Arg Phe Leu Glu Gly Gly Gly Gly Thr Gly Ser Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile 1060 1.065 1070 Ala Ala Ala Tyr Asn Asn Gly Gln Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gln Thr Ala Glu Glu Thr Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gln Gly Leu Ala Glu Gln Ala Ala Leu Ser Gly Leu Phe Gln Pro Tyr Asn Val Gly Leu Glu His His His His His His <210> 25 <211> 274 <212> PRT
<213> Artificial Sequence <220>
<223> 741 <400> 25 Val Asn Arg Thr Ala Phe Cys Cys Leu Ser Leu Thr Thr Ala Leu Ile Leu Thr Ala Cys Ser Ser Gly Gly Gly Gly Val Ala Ala Asp Ile Gly Ala Gly Leu Ala Asp Ala Leu Thr Ala Pro Leu Asp His Lys Asp Lys Gly Leu Gln Ser Leu Thr Leu Asp Gin Ser Val Arg Lys Asn Glu Lys Leu Lys Leu Ala Ala Gln Gly Ala Glu Lys Thr Tyr Gly Asn Gly Asp Ser Leu Asn Thr Gly Lys Leu Lys Asn Asp Lys Val Ser Arg Phe Asp Phe Ile Arg Gln Ile Glu Val Asp Gly Gln Leu Ile Thr Leu Glu Ser Gly Glu Phe Gln Val Tyr Lys Gln Ser His Ser Ala Leu Thr Ala Phe Gln Thr Glu Gln Ile Gln Asp Ser Glu His Ser Gly Lys Met Val Ala Lys Arg Gln Phe Arg Ile Gly Asp Ile Ala Gly Glu His Thr Ser Phe Asp Lys Leu Pro Glu Gly Gly Arg Ala Thr Tyr Arg Gly Thr Ala Phe Gly Ser Asp Asp Ala Gly Gly Lys Leu Thr Tyr Thr Ile Asp Phe Ala Ala Lys Gln Gly Asn Gly Lys Ile Glu His Leu Lys Ser Pro Glu Leu Asn Val Asp Leu Ala Ala Ala Asp Ile Lys Pro Asp Gly Lys Arg His Ala Val Ile Ser Gly Ser Val Leu Tyr Asn Gln Ala Glu Lys Gly Ser Tyr Ser Leu Gly Ile Phe Gly Gly Lys Ala Gin Glu Val Ala Gly Ser Ala Glu Val Lys Thr Val Asn Gly Ile Arg His Ile Gly Leu Ala Ala Lys Gln <210> 26 <211> 248 <212> PRT
<213> Artificial Sequence <220>
<223> deltaG741 <400> 26 Val Ala Ala Asp Ile Gly Ala Gly Leu Ala Asp Ala Leu Thr Ala Pro Leu Asp His Lys Asp Lys Gly Leu Gln Ser Leu Thr Leu Asp Gln Ser Val Arg Lys Asn Glu Lys Leu Lys Leu Ala Ala Gln Gly Ala Glu Lys Thr Tyr Gly Asn Gly Asp Ser Leu Asn Thr Gly Lys Leu Lys Asn Asp Lys Val Ser Arg Phe Asp Phe Ile Arg Gln Ile Glu Val Asp Gly Gln Leu Ile Thr Leu Glu Ser Gly Glu Phe Gln Val Tyr Lys Gln Ser His Ser Ala Leu Thr Ala Phe Gln Thr Glu Gln Ile Gln Asp Ser Glu His Ser Gly Lys Met Val Ala Lys Arg Gln Phe Arg Ile Gly Asp Ile Ala Gly Glu His Thr Ser Phe Asp Lys Leu Pro Glu Gly Gly Arg Ala Thr Tyr Arg Gly Thr Ala Phe Gly Ser Asp Asp Ala Gly Gly Lys Leu Thr Tyr Thr Ile Asp Phe Ala Ala Lys Gln Gly Asn Gly Lys Ile Glu His Leu Lys Ser Pro Glu Leu Asn Val Asp Leu Ala Ala Ala Asp Ile Lys Pro Asp Gly Lys Arg His Ala Val Ile Ser Gly Ser Val Leu Tyr Asn Gln Ala Glu Lys Gly Ser Tyr Ser Leu Gly Ile Phe Gly Gly Lys Ala Gln Glu Val Ala Gly Ser Ala Glu Val Lys Thr Val Asn Gly Ile Arg His Ile Gly Leu Ala Ala Lys Gln <210> 27 <211> 1947 <212> DNA
<213> Artificial Sequence <220>
<223> deltaG741-961 <400> 27 atggtcgccg ccgacatcgg tgcggggctt gccgatgcac taaccgcacc gctcgaccat 60 aaagacaaag gtttgcagtc tttgacgctg gatcagtccg tcaggaaaaa cgagaaactg 120 aagctggcgg cacaaggtgc ggaaaaaact tatggaaacg gtgacagcct caatacgggc 180 aaattgaaga acgacaaggt cagccgtttc gactttatcc gccaaatcga agtggacggg 240 cagctcatta ccttggagag tggagagttc caagtataca aacaaagcca ttccgcctta 300 accgcctttc agaccgagca aatacaagat tcggagcatt ccgggaagat ggttgcgaaa 360 cgccagttca gaatcggcga catagcgggc gaacatacat cttttgacaa gcttcccgaa 420 ggcggcaggg cgacatatcg cgggacggcg ttcggttcag acgatgccgg cggaaaactg 480 acctacacca tagatttcgc cgccaagcag ggaaacggca aaatcgaaca tttgaaatcg 540 ccagaactca atgtcgacct ggccgccgcc gatatcaagc cggatggaaa acgccatgcc 600 gtcatcagcg gttccgtcct ttacaaccaa gccgagaaag gcagttactc cctcggtatc 660 tttggcggaa aagcccagga agttgccggc agcgcggaag tgaaaaccgt aaacggcata 720 cgccatatcg gccttgccgc caagcaactc gagggtggcg gaggcactgg atccgccaca 780 aacgacgacg atgttaaaaa agctgccact gtggccattg ctgctgccta caacaatggc 840 caagaaatca acggtttcaa agctggagag accatctacg acattgatga agacggcaca 900 attaccaaaa aagacgcaac tgcagccgat gttga.agccg acgactttaa aggtctgggt 960 ctgaaaaaag tcgtgactaa cctgaccaaa accgtcaatg aaaacaaaca aaacgtcgat 1020 gccaaagtaa aagctgcaga atctgaaata gaaaagttaa caaccaagtt agcagacact 1080 gatgccgctt tagcagatac tgatgccgct ctggatgcaa ccaccaacgc cttgaataaa 1140 ttgggagaaa atataacgac atttgctgaa gagactaaga caaatatcgt aaaaattgat 1200 gaaaaattag aagccgtggc tgataccgtc gacaagcatg ccgaagcatt caacgatatc 1260 gccgattcat tggatgaaac caacactaag gcagacgaag ccgtcaaaac cgccaatgaa 1320 gccaaacaga cggccgaaga aaccaaacaa aacgtcgatg ccaaagtaaa agctgcagaa 1380 actgcagcag gcaaagccga agctgccgct ggcacagcta atactgcagc cgacaaggcc 1440 gaagctgtcg ctgcaaaagt taccgacatc aaagctgata tcgctacgaa caaagataat 1500 attgctaaaa aagcaaacag tgccgacgtg tacaccagag aagagtctga cagcaaattt 1560 gtcagaattg atggtctgaa cgctactacc gaaaaattgg acacacgctt ggcttctgct 1620 gaaaaatcca ttgccgatca cgatactcgc ctgaacggtt tggataaaac agtgtcagac 1680 ctgcgcaaag aaacccgcca aggccttgca gaacaagccg cgctctccgg tctgttccaa 1740 ccttacaacg tgggtcggtt caatgtaacg gctgcagtcg gcggctacaa atccgaatcg 1800 gcagtcgcca tcggtaccgg cttccgcttt accgaaaact ttgccgccaa agcaggcgtg 1860 gcagtcggca cttcgtccgg ttcttccgca gcctaccatg tcggcgtcaa ttacgagtgg 1920 ctcgagcacc accaccacca ccactga 1947 <210> 28 <211> 648 <212> PRT
<213> Artificial Sequence <220>
<223> deltaG741-961 <400> 28 Met Val Ala Ala Asp Ile Gly Ala Gly Leu Ala Asp Ala Leu Thr Ala Pro Leu Asp His Lys Asp Lys Gly Leu Gln Ser Leu Thr Leu Asp Gln Ser Val Arg Lys Asn Glu Lys Leu Lys Leu Ala Ala Gln Gly Ala Glu Lys Thr Tyr Gly Asn Gly Asp Ser Leu Asn Thr Gly Lys Leu Lys Asn Asp Lys Val Ser Arg Phe Asp Phe Ile Arg Gln Ile Glu Val Asp Gly Gln Leu Ile Thr Leu Glu Ser Gly Glu Phe Gln Val Tyr Lys Gln Ser His Ser Ala Leu Thr Ala Phe Gln Thr Glu Gln Ile Gln Asp Ser Glu His Ser Gly Lys Met Val Ala Lys Arg Gln Phe Arg Ile Gly Asp Ile Ala Gly Glu His Thr Ser Phe Asp Lys Leu Pro Glu Gly Gly Arg Ala Thr Tyr Arg Gly Thr Ala Phe Gly Ser Asp Asp Ala Gly Gly Lys Leu Thr Tyr Thr Ile Asp Phe Ala Ala Lys Gln Gly Asn Gly Lys Ile Glu His Leu Lys Ser Pro Glu Leu Asn Val Asp Leu Ala Ala Ala Asp Ile Lys Pro Asp Gly Lys Arg His Ala Val Ile Ser Gly Ser Val Leu Tyr Asn Gln Ala Glu Lys Gly Ser Tyr Ser Leu Gly Ile Phe Gly Gly Lys Ala Gln Glu Val Ala Gly Ser Ala Glu Val Lys Thr Val Asn Gly Ile Arg His Ile Gly Leu Ala Ala Lys Gln Leu Glu Gly Gly Gly Gly Thr Gly Ser Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile Ala Ala Ala Tyr Asn Asn Gly Gln Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gln Thr Ala Glu Glu Thr Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gln Gly Leu Ala Glu Gin Ala Ala Leu Ser Gly Leu Phe Gln Pro Tyr Asn Val Gly Arg Phe Asn Val Thr Ala Ala Val Gly Gly Tyr Lys Ser Glu Ser Ala Val Ala Ile Gly Thr Gly Phe Arg Phe Thr Glu Asn Phe Ala Ala Lys Ala Gly Val Ala Val Gly Thr Ser Ser Gly Ser Ser Ala Ala Tyr His Val Gly Val Asn Tyr Glu Trp Leu Glu His His His His His His <210> 29 <211> 1782 <212> DNA
<213> Artificial Sequence <220>
<223> deltaG741-961c <400> 29 atggtcgccg ccgacatcgg tgcggggctt gccgatgcac taaccgcacc gctcgaccat 60 aaagacaaag gtttgcagtc tttgacgctg gatcagtccg tcaggaaaaa cgagaaactg 120 aagctggcgg cacaaggtgc ggaaaaaact tatggaaacg gtgacagcct caatacgggc 180 aaattgaaga acgacaaggt cagccgtttc gactttatcc gccaaatcga agtggacggg 240 cagctcatta ccttggagag tggagagttc caagtataca aacaaagcca ttccgcctta 300 accgcctttc agaccgagca aatacaagat tcggagcatt ccgggaagat ggttgcgaaa 360 cgccagttca gaatcggcga catagcgggc gaacatacat cttttgacaa gcttcccgaa 420 ggcggcaggg cgacatatcg cgggacggcg ttcggttcag acgatgccgg cggaaaactg 480 acctacacca tagatttcgc cgccaagcag ggaaacggca aaatcgaaca tttgaaatcg 540 ccagaactca atgtcgacct ggccgccgcc gatatcaagc cggatggaaa acgccatgcc 600 gtcatcagcg gttccgtcct ttacaaccaa gccgagaaag gcagttactc cctcggtatc 660 tttggcggaa aagcccagga agttgccggc agcgcggaag tgaaaaccgt aaacggcata 720 cgccatatcg gccttgccgc caagcaactc gagggtggcg gaggcactgg atccgccaca 780 aacgacgacg atgttaaaaa agctgccact gtggccattg ctgctgccta caacaatggc 840 caagaaatca acggtttcaa agctggagag accatctacg acattgatga agacggcaca 900 attaccaaaa aagacgcaac tgcagccgat gttgaagccg acgactttaa aggtctgggt 960 ctgaaaaaag tcgtgactaa cctgaccaaa accgtcaatg aaaacaaaca aaacgtcgat 1020 gccaaagtaa aagctgcaga atctgaaata gaaaagttaa caaccaagtt agcagacact 1080 gatgccgctt tagcagatac tgatgccgct ctggatgcaa ccaccaacgc cttgaataaa 1140 ttgggagaaa atataacgac atttgctgaa gagactaaga caaatatcgt aaaaattgat 1200 gaaaaattag aagccgtggc tgataccgtc gacaagcatg ccgaagcatt caacgatatc 1260 gccgattcat tggatgaaac caacactaag gcagacgaag ccgtcaaaac cgccaatgaa 1320 gccaaacaga cggccgaaga aaccaaacaa aacgtcgatg ccaaagtaaa agctgcagaa 1380 actgcagcag gcaaagccga agctgccgct ggcacagcta atactgcagc cgacaaggcc 1440 gaagctgtcg ctgcaaaagt taccgacatc aaacctcata tcgctacgaa caaagataat 1500 attgctaaaa aagcaaacag tgccgacgtg tacaccagag aagagtctga cagcaaattt 1560 gtcagaattg atggtctgaa cgctactacc gaaaaattgg acacacgctt ggcttctgct 1620 gaaaaatcca ttgccgatca cgatactcgc ctgaacggtt tggataaaac agtgtcagac 1680 ctgcgcaaag aaacccgcca aggccttgca gaacaagccg cgctctccgg tctgttccaa 1740 ccttacaacg tgggtctcga gcaccaccac caccaccact ga 1782 <210> 30 <211> 593 <212> PRT
<213> Artificial Sequence <220>
<223> deltaG741-961c <400> 30 Met Val Ala Ala Asp Ile Gly Ala Gly Leu Ala Asp Ala Leu Thr Ala Pro Leu Asp His Lys Asp Lys Gly Leu Gln Ser Leu Thr Leu Asp Gln Ser Val Arg Lys Asn Glu Lys Leu Lys Leu Ala Ala Gln Gly Ala Glu Lys Thr Tyr Gly Asn Gly Asp Ser Leu Asn Thr Gly Lys Leu Lys Asn Asp Lys Val Ser Arg Phe Asp Phe Ile Arg Gln Ile Glu Val Asp Gly Gln Leu Ile Thr Leu Glu Ser Gly Glu Phe Gln Val Tyr Lys Gln Ser His Ser Ala Leu Thr Ala Phe Gln Thr Glu Gln Ile Gln Asp Ser Glu His Ser Gly Lys Met Val Ala Lys Arg Gln Phe Arg Ile Gly Asp Ile Ala Gly Glu His Thr Ser Phe Asp Lys Leu Pro Glu Gly Gly Arg Ala Thr Tyr Arg Gly Thr Ala Phe Gly Ser Asp Asp Ala Gly Gly Lys Leu Thr Tyr Thr Ile Asp Phe Ala Ala Lys Gln Gly Asn Gly Lys Ile Glu His Leu Lys Ser Pro Glu Leu Asn Val Asp Leu Ala Ala Ala Asp Ile Lys Pro Asp Gly Lys Arg His Ala Val Ile Ser Gly Ser Val Leu Tyr Asn Gln Ala Glu Lys Gly Ser Tyr Ser Leu Gly Ile Phe Gly Gly Lys Ala Gln Glu Val Ala Gly Ser Ala Glu Val Lys Thr Val Asn Gly Ile Arg His Ile Gly Leu Ala Ala Lys Gln Leu Glu Gly Gly Gly Gly Thr Gly Ser Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile Ala Ala Ala Tyr Asn Asn Gly Gln Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gln Thr Ala Glu Glu Thr Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gln Gly Leu Ala Glu Gln Ala Ala Leu Ser Gly Leu Phe Gln Pro Tyr Asn Val Gly Leu Glu His His His His His His <210> 31 <211> 3939 <212> DNA
<213> Artificial Sequence <220>
<223> deltaG741-983 <400> 31 atggtcgccg ccgacatcgg tgcggggctt gccgatgcac taaccgcacc gctcgaccat 60 aaagacaaag gtttgcagtc tttgacgctg gatcagtccg tcaggaaaaa cgagaaactg 120 aagctggcgg cacaaggtgc ggaaaaaact tatggaaacg gtgacagcct caatacgggc 180 aaattgaaga acgacaaggt cagccgtttc gactttatcc gccaaatcga agtggacggg 240 cagctcatta ccttggagag tggagagttc caagtataca aacaaagcca ttccgcctta 300 accgcctttc agaccgagca aatacaagat tcggagcatt ccgggaagat ggttgcgaaa 360 cgccagttca gaatcggcga catagcgggc gaacatacat cttttgacaa gcttcccgaa 420 ggcggcaggg cgacatatcg cgggacggcg ttcggttcag acgatgccgg cggaaaactg 480 acctacacca tagatttcgc cgccaagcag ggaaacggca aaatcgaaca tttgaaatcg 540 ccagaactca atgtcgacct ggccgccgcc gatatcaagc cggatggaaa acgccatgcc 600 gtcatcagcg gttccgtcct ttacaaccaa gccgagaaag gcagttactc cctcggtatc 660 tttggcggaa aagcccagga agttgccggc agcgcggaag tgaaaaccgt aaacggcata 720 cgccatatcg gccttgccgc caagcaactc gagggatccg gcggaggcgg cacttctgcg 780 cccgacttca atgcaggcgg taccggtatc ggcagcaaca gcagagcaac aacagcgaaa 840 tcagcagcag tatcttacac cggtatcaag aacgaaatgt gcaaagacag aagcatgctc 900 tgtgccggtc gggatgacgt ttcggttaca gacagggatg ccaaaatcaa tgcccccccc 960 ccgaatctgc ataccggaga ctttccaaac ccaaatgacg catacaagaa tttgatcaac 1020 ctcaaacctg caattgaagc aggctataca ggacgcgggg tagaggtagg tatcgtcgac 1080 acaggcgaat ccgtcggcag catatccttt cccgaactgt atggcagaaa agaacacggc 1140 tataacgaaa attacaaaaa ctatacggcg tatatgcgga aggaagcgcc tgaagacgga 1200 ggcggtaaag acattgaagc ttttttcgac gatgaggccg ttatagagac tgaagcaaag 1260 ccgacggata tccgccacgt aaaagaaatc ggacacatcg atttggtctc ccatattatt 1320 ggcgggcgtt ccgtggacgg cagacctgca ggcggtattg cgcccgatgc gacgctacac 1380 ataatgaata cgaatgatga aaccaagaac gaaatgatgg ttgcagccat ccgcaatgca 1440 tgggtcaagc tgggtcaacg tggcgtgcgc atcgtcaata acagttttgg aacaacatcg 1500 agggcaggca ctgccgacct tttccaaata gccaattcgg aggagcagta ccgccaagcg 1560 ttgctcgact attccggcgg tgataaaaca gacgagggta tccgcctgat gcaacagagc 1620 gattacggca acctgtccta ccacatccgt aataaaaaca tgcttttcat cttttcgaca 1680 ggcaatgacg cacaagctca gcccaacaca tatgccctat tgccatttta tgaaaaagac 1740 gctcaaaaag gcattatcac agtcgcaggc gtagaccgca gtggagaaaa gttcaaacgg 1800 gaaatgtatg gagaaccggg tacagaaccg cttgagtatg gctccaacca ttgcggaatt 1860 actgccatgt ggtgcctgtc ggcaccctat gaagcaagcg tccgtttcac ccgtacaaac 1920 ccgattcaaa ttgccggaac atccttttcc gcacccatcg taaccggcac ggcggctctg 1980 ctgctgcaga aatacccgtg gatgagcaac gacaacctgc gtaccacgtt gctgacgacg 2040 gctcagaaca tcggtgcagt cggcgtggac agcaagttcg gctggggact gctggatgcg 2100 ggtaaggcca tgaacggacc cgcgtccttt ccgttcggcg actttaccgc cgataggaaa 2160 ggtacatccg atattgccta ctccttccgt aacgacattt caggcacggg cggcctgatc 2220 aaaaaaggcg gcagccaact gcaactgcac ggcaacaaca cctatacggg caaaaccatt 2280 atcgaaggcg gttcgctggt gttgtacggc aacaacaaat cggatatgcg cgtcgaaacc 2340 aaaggtgcgc tgatttataa cggggcggca tccggcggca gcctgaacag cgacggcatt 2400 gtctatctgg cagataccga ccaatccggc gcaaacgaaa ccgtacacat caaaggcagt 2460 ctgcagctgg acggcaaagg tacgctgtac acacgtttgg gcaaactgct gaaagtggac 2520 ggtacggcga ttatcggcgg caagctgtac atgtcggcac gcggcaaggg ggcaggctat 2580 ctcaacagta ccggacgacg tgttcccttc ctgagtgccg ccaaaatcgg gcaggattat 2640 tctttcttca caaacatcga aaccgacggc ggcctgctgg cttccctcga cagcgtcgaa 2700 aaaacagcgg gcagtgaagg cgacacgctg tcctattatg tccgtcgcgg caatgcggca 2760 cggactgctt cggcagcggc acattccgcg cccgccggtc tgaaacacgc cgtagaacag 2820 ggcggcagca atctggaaaa cctgatggtc gaactggatg cctccgaatc atccgcaaca 2880 cccgagacgg ttgaaactgc ggcagccgac cgcacagata tgccgggcat ccgcccctac 2940 ggcgcaactt tccgcgcagc ggcagccgta cagcatgcga atgccgccga cggtgtacgc 3000 atcttcaaca gtctcgccgc taccgtctat gccgacagta ccgccgccca tgccgatatg 3060 cagggacgcc gcctgaaagc cgtatcggac gggttggacc acaacggcac gggtctgcgc 3120 gtcatcgcgc aaacccaaca ggacggtgga acgtgggaac agggcggtgt tgaaggcaaa 3180 atgcgcggca gtacccaaac cgtcggcatt gccgcgaaaa ccggcgaaaa tacgacagca 3240 gccgccacac tgggcatggg acgcagcaca tggagcgaaa acagtgcaaa tgcaaaaacc 3300 gacagcatta gtctgtttgc aggcatacgg cacgatgcgg gcgatatcgg ctatctcaaa 3360 ggcctgttct cctacggacg ctacaaaaac agcatcagcc gcagcaccgg tgcggacgaa 3420 catgcggaag gcagcgtcaa cggcacgctg atgcagctgg gcgcactggg cggtgtcaac 3480 gttccgtttg ccgcaacggg agatttgacg gtcgaaggcg gtctgcgcta cgacctgctc 3540 aaacaggatg cattcgccga aaaaggcagt gctttgggct ggagcggcaa cagcctcact 3600 gaaggcacgc tggtcggact cgcgggtctg aagctgtcgc aacccttgag cgataaagcc 3660 gtcctgtttg caacggcggg cgtggaacgc gacctgaacg gacgcgacta cacggtaacg 3720 ggcggcttta ccggcgcgac tgcagcaacc ggcaagacgg gggcacgcaa tatgccgcac 3780 acccgtctgg ttgccggcct gggcgcggat gtcgaattcg gcaacggctg gaacggcttg 3840 gcacgttaca gctacgccgg ttccaaacag tacggcaacc acagcggacg agtcggcgta 3900 ggctaccggt tcctcgagca ccaccaccac caccactga 3939 <210> 32 <211> 1312 <212> PRT
<213> Artificial Sequence <220>
<223> deltaG741-983 <400> 32 Met Val Ala Ala Asp Ile Gly Ala Gly Leu Ala Asp Ala Leu Thr Ala Pro Leu Asp His Lys Asp Lys Gly Leu Gln Ser Leu Thr Leu Asp Gln Ser Val Arg Lys Asn Glu Lys Leu Lys Leu Ala Ala Gln Gly Ala Glu Lys Thr Tyr Gly Asn Gly Asp Ser Leu Asn Thr Gly Lys Leu Lys Asn Asp Lys Val Ser Arg Phe Asp Phe Ile Arg Gln Ile Glu Val Asp Gly Gln Leu Ile Thr Leu Glu Ser Gly Glu Phe Gln Val Tyr Lys Gln Ser His Ser Ala Leu Thr Ala Phe Gln Thr Glu Gln Ile Gln Asp Ser Glu His Ser Gly Lys Net Val Ala Lys Arg Gln Phe Arg Ile Gly Asp Ile Ala Gly Glu His Thr Ser Phe Asp Lys Leu Pro Glu Gly Gly Arg Ala Thr Tyr Arg Gly Thr Ala Phe Gly Ser Asp Asp Ala Gly Gly Lys Leu Thr Tyr Thr Ile Asp Phe Ala Ala Lys Gln Gly Asn Gly Lys Ile Glu His Leu Lys Ser Pro Glu Leu Asn Val Asp Leu Ala Ala Ala Asp Ile Lys Pro Asp Gly Lys Arg His Ala Val Ile Ser Gly Ser Val Leu Tyr Asn Gln Ala Glu Lys Gly Ser Tyr Ser Leu Gly Ile Phe Gly Gly Lys Ala Gln Glu Val Ala Gly Ser Ala Glu Val Lys Thr Val Asn Gly Ile Arg His Ile Gly Leu Ala Ala Lys Gln Leu Glu Gly Ser Gly Gly Gly Gly Thr Ser Ala Pro Asp Phe Asn Ala Gly Gly Thr Gly Ile Gly Ser Asn Ser Arg Ala Thr Thr Ala Lys Ser Ala Ala Val Ser Tyr Ala Gly Ile Lys Asn Glu Met Cys Lys Asp Arg Ser Met Leu Cys Ala Gly Arg Asp Asp Val Ala Val Thr Asp Arg Asp Ala Lys Ile Asn Ala Pro Pro Pro Asn Leu His Thr Gly Asp Phe Pro Asn Pro Asn Asp Ala Tyr Lys Asn Leu Ile Asn Leu Lys Pro Ala Ile Glu Ala Gly Tyr Thr Gly Arg Gly Val Glu Val Gly Ile Val Asp Thr Gly Glu Ser Val Gly Ser Ile Ser Phe Pro Glu Leu Tyr Gly Arg Lys Glu His Gly Tyr Asn Glu Asn Tyr Lys Asn Tyr Thr Ala Tyr Met Arg Lys Glu Ala Pro Glu Asp Gly Gly Gly Lys Asp Ile Glu Ala Ser Phe Asp Asp Glu Ala Val Ile Glu Thr Glu Ala Lys Pro Thr Asp Ile Arg His Val Lys Glu Ile Gly His Ile Asp Leu Val Ser His Ile Ile Gly Gly Arg Ser Val Asp Gly Arg Pro Ala Gly Gly Ile Ala Pro Asp Ala Thr Leu His Ile Met Asn Thr -IIs-Asn Asp Glu Thr Lys Asn Glu Met Met Val Ala Ala Ile Arg Asn Ala Trp Val Lys Leu Gly Glu Arg Gly Val Arg Ile Val Asn Asn Ser Phe Gly Thr Thr Ser Arg Ala Gly Thr Ala Asp Leu Phe Gln Ile Ala Asn Ser Glu Glu Gin Tyr Arg Gin Ala Leu Leu Asp Tyr Ser Gly Gly Asp Lys Thr Asp Glu Gly Ile Arg Leu Met Gln Gln Ser Asp Tyr Gly Asn Leu Ser Tyr His Ile Arg Asn Lys Asn Met Leu Phe Ile Phe Ser Thr Gly Asn Asp Ala Gln Ala Gln Pro Asn Thr Tyr Ala Leu Leu Pro Phe Tyr Glu Lys Asp Ala Gin Lys Gly Ile Ile Thr Val Ala Gly Val Asp Arg Ser Gly Glu Lys Phe Lys Arg Glu Met Tyr Gly Glu Pro Gly Thr Glu Pro Leu Glu Tyr Gly Ser Asn His Cys Gly Ile Thr Ala Met Trp Cys Leu Ser Ala Pro Tyr Glu Ala Ser Val. Arg Phe Thr Arg Thr Asn Pro Ile Gln Ile Ala Gly Thr Ser Phe Ser Ala Pro Ile Val Thr Gly Thr Ala Ala Leu Leu Leu Gln Lys Tyr Pro Trp Met Ser Asn Asp Asn Leu Arg Thr Thr Leu Leu Thr Thr Ala Gln Asp Ile Gly Ala Val Gly Val Asp Ser Lys Phe Gly Trp Gly Leu Leu Asp Ala Gly Lys Ala Met Asn Gly Pro Ala Ser Phe Pro Phe Gly Asp Phe Thr Ala Asp Thr Lys Gly Thr Ser Asp Ile Ala Tyr Ser Phe Arg Asn Asp Ile Ser Gly Thr Gly Gly Leu Ile Lys Lys Gly Gly Ser Gln Leu Gln Leu His Gly Asn Asn Thr Tyr Thr Gly Lys Thr Ile Ile Glu Gly Gly Ser Leu Val Leu Tyr Gly Asn Asn Lys Ser Asp Met Arg Val Glu Thr Lys Gly Ala Leu Ile Tyr Asn Gly Ala Ala Ser Gly Gly Ser Leu Asn Ser Asp Gly Ile Val Tyr Leu Ala Asp Thr Asp Gln Ser Gly Ala Asn Glu Thr Val His Ile Lys Gly Ser Leu Gln Leu Asp Gly Lys Gly Thr Leu Tyr Thr Arg Leu Gly Lys Leu Leu Lys Val Asp Gly Thr Ala Ile Ile Gly Gly Lys Leu Tyr Met Ser Ala Arg Gly Lys Gly Ala Gly Tyr Leu Asn Ser Thr Gly Arg Arg Val Pro Phe Leu Ser Ala Ala Lys Ile Gly Gln Asp Tyr Ser Phe Phe Thr Asn Ile Glu Thr Asp Gly Gly Leu Leu Ala Ser Leu Asp Ser Val Glu Lys Thr Ala Gly Ser Glu Gly Asp Thr Leu Ser Tyr Tyr Val Arg Arg Gly Asn Ala Ala Arg Thr Ala Ser Ala Ala Ala His Ser Ala Pro Ala Gly Leu Lys His Ala Val Glu Gln Gly Gly Ser Asn Leu Glu Asn Leu Met Val Glu Leu Asp Ala Ser Glu Ser Ser Ala Thr Pro Glu Thr Val Glu Thr Ala Ala Ala Asp Arg Thr Asp Met Pro Gly Ile Arg Pro Tyr Gly Ala Thr Phe Arg Ala Ala Ala Ala Val Gln His Ala Asn Ala Ala Asp Gly Val Arg Ile Phe Asn Ser Leu Ala Ala Thr Val Tyr Ala Asp Ser Thr Ala Ala His Ala Asp Met Gln Gly Arg Arg Leu Lys Ala Val Ser Asp Gly Leu Asp His Asn Gly Thr Gly Leu Arg Val Ile Ala Gln Thr Gln Gln Asp Gly Gly Thr Trp Glu Gln Gly Gly Val Glu Gly Lys Met Arg Gly Ser Thr Gln Thr Val Gly Ile Ala Ala Lys Thr Gly Glu Asn Thr Thr Ala Ala Ala Thr Leu Gly Met Gly Arg Ser Thr Trp Ser Glu Asn Ser Ala Asn Ala Lys Thr Asp Ser Ile Ser Leu Phe Ala Gly Ile Arg His Asp Ala Gly Asp Ile Gly Tyr Leu Lys Gly Leu Phe Ser Tyr Gly Arg Tyr Lys Asn Ser Ile Ser Arg Ser Thr Gly Ala Asp Glu His Ala Glu Gly Ser Val Asn Gly Thr Leu Met Gln Leu Gly Ala Leu Gly Gly Val Asn Val Pro Phe Ala Ala Thr Gly Asp Leu Thr Val Glu Gly Gly Leu Arg Tyr Asp Leu Leu Lys Gln Asp Ala Phe Ala Glu Lys Gly Ser Ala Leu Gly Trp Ser Gly Asn Ser Leu Thr Glu Gly Thr Leu Val Gly Leu Ala Gly Leu Lys Leu Ser Gln Pro Leu Ser Asp Lys Ala Val Leu Phe Ala Thr Ala Gly Val Glu Arg Asp Leu Asn Gly Arg Asp Tyr Thr Val Thr Gly Gly Phe Thr Gly Ala Thr Ala Ala Thr Gly Lys Thr Gly Ala Arg Asn Met Pro His Thr Arg Leu Val Ala Gly Leu Gly Ala Asp Val Glu Phe Gly Asn Gly Trp Asn Gly Leu Ala Arg Tyr Ser Tyr Ala Gly Ser Lys Gln Tyr Gly Asn His Ser Gly Arg Val Gly Val Gly Tyr Arg Phe Leu Glu His His His His His His <210> 33 <211> 2028 <212> DNA
<213> Artificial Sequence <220>
<223> deltaG741-ORF46.1 <400> 33 atggtcgccg ccgacatcgg tgcggggctt gccgatgcac taaccgcacc gctcgaccat 60 aaagacaaag gtttgcagtc tttgacgctg gatcagtccg tcaggaaaaa cgagaaactg 120 aagctggcgg cacaaggtgc ggaaaaaact tatggaaacg gtgacagcct caatacgggc 180 aaattgaaca acgacaaggt cagccgtttc gactttatcc gccaaatcga agtggacggg 240 cagctcatta ccttggagag tggagagttc caagtataca aacaaagcca ttccgcctta 300 accgcctttc agaccgagca aatacaagat tcggagcatt ccgggaagat ggttgcgaaa 360 cgccagttca gaatcggcga catagcgggc gaacatacat cttttgacaa gcttcccgaa 420 ggcggcaggg cgacatatcg cgggacggcg ttcggttcag acgatgccgg cggaaaactg 480 acctacacca tagatttcgc cgccaagcag ggaaacggca aaatcgaaca tttgaaatcg 540 ccagaactca atgtcgacct ggccgccgcc gatatcaagc cggatggaaa acgccatgcc 600 gtcatcagcg gttccgtcct ttacaaccaa gccgagaaag gcagttactc cctcggtatc 660 tttggcggaa aagcccagga agttgccggc agcgcggaag tgaaaaccgt aaacggcata 720 cgccatatcg gccttgccgc caagcaactc gacggtggcg gaggcactgg atcctcagat 780 ttggcaaacg attcttttat ccggcaggtt ctcgaccgtc agcatttcga acccgacggg 840 aaataccacc tattcggcag caggggggaa cttgccgagc gcagcggcca tatcggattg 900 ggaaaaatac aaagccatca gttgggcaac ctgatgattc aacaggcggc cattaaagga 960 aatatcggct acattgtccg cttttccgat cacgggcacg aagtccattc ccccttcgac 1020 aaccatgcct cacattccga ttctgatgaa gccggtagtc ccgttgacgg atttagcctt 1080 taccgcatcc attgggacgg atacgaacac catcccgccg acggctatga cgggccacag 1140 ggcggcggct atcccgctcc caaaggcgcg agggatatat acagctacga cataaaaggc 1200 gttgcccaaa atatccgcct caacctgacc gacaaccgca gcaccggaca acggcttgcc 1260 gaccgtttcc acaatgccgg tagtatgctg acgcaaggag taggcgacgg attcaaacgc 1320 gccacccgat acagccccga gctggacaga tcgggcaatg ccgccgaagc cttcaacggc 1380 actgcagata tcgttaaaaa catcatcggc gcggcaggag aaattgtcgg cgcaggcgat 1440 gccgtgcagg gcataagcga aggctcaaac attgctgtca tgcacggctt gggtctgctt 1500 tccaccgaaa acaagatggc gcgcatcaac gatttggcag atatggcgca actcaaagac 1560 tatgccgcag cagccatccg cgattgggca gtccaaaacc ccaatgccgc acaaggcata 1620 gaagccgtca gcaatatctt tatggcagcc atccccatca aagggattgg agctgttcgg 1680 ggaaaatacg gcttgggcgg catcacggca catcctatca agcggtcgca gatgggcgcg 1740 atcgcattgc cgaaagggaa atccgccgtc agcgacaatt ttgccgatgc ggcatacgcc 1800 aaatacccgt ccccttacca ttcccgaaat atccgttcaa acttggagca gcgttacggc 1860 aaagaaaaca tcacctcctc aaccgtgccg ccgtcaaacg gcaaaaatgt caaactggca 1920 gaccaacgcc acccgaagac aggcgtaccg tttgacggta aagggtttcc gaattttgag 1980 aagcacgtga aatatgatac gctcgagcac caccaccacc accactga 2028 <210> 34 <211> 675 <212> PRT
<213> Artificial Sequence <220>
<223> deltaG741-ORF46.1 <400> 34 Met Val Ala Ala Asp Ile Gly Ala Gly Leu Ala Asp Ala Leu Thr Ala Pro Leu Asp His Lys Asp Lys Gly Leu Gln Ser Leu Thr Leu Asp Gln Ser Val Arg Lys Asn Glu Lys Leu Lys Leu Ala Ala Gln Gly Ala Glu Lys Thr Tyr Gly Asn Gly Asp Ser Leu Asn Thr Gly Lys Leu Lys Asn Asp Lys Val Ser Arg Phe Asp Phe Ile Arg Gln Ile Glu Val Asp Gly Gln Leu Ile Thr Leu Glu Ser Gly Glu Phe Gln Val Tyr Lys Gln Ser His Ser Ala Leu Thr Ala Phe Gln Thr Glu Gln Ile Gln Asp Ser Glu His Ser Gly Lys Met Val Ala Lys Arg Gln Phe Arg Ile Gly Asp Ile Ala Gly Glu His Thr Ser Phe Asp Lys Leu Pro Glu Gly Gly Arg Ala Thr Tyr Arg Gly Thr Ala Phe Gly Ser Asp Asp Ala Gly Gly Lys Leu Thr Tyr Thr Ile Asp Phe Ala Ala Lys Gln Gly Asn Gly Lys Ile Glu His Leu Lys Ser Pro Glu Leu Asn Val Asp Leu Ala Ala Ala Asp Ile Lys Pro Asp Gly Lys Arg His Ala Val Ile Ser Gly Ser Val Leu Tyr Asn Gln Ala Glu Lys Gly Ser Tyr Ser Leu Gly Ile Phe Gly Gly Lys Ala Gln Glu Val Ala Gly Ser Ala Glu Val Lys Thr Val Asn Gly Ile Arg His Ile Gly Leu Ala Ala Lys Gln Leu Asp Gly Gly Gly Gly Thr Gly Ser Ser Asp Leu Ala Asn Asp Ser Phe Ile Arg Gln Val Leu Asp Arg Gln His Phe Glu Pro Asp Gly Lys Tyr His Leu Phe Gly Ser Arg Gly Glu Leu Ala Glu Arg Ser Gly His Ile Gly Leu Gly Lys Ile Gln Ser His Gln Leu Gly Asn Leu Met Ile Gin Gln Ala Ala Ile Lys Gly Asn Ile Gly Tyr Ile Val Arg Phe Ser Asp His Gly His Glu Val His Ser Pro Phe Asp Asn His Ala Ser His Ser Asp Ser Asp Glu Ala Gly Ser Pro Val Asp Gly Phe Ser Leu Tyr Arg Ile His Trp Asp Gly Tyr Glu His His Pro Ala Asp Gly Tyr Asp Gly Pro Gln Gly Gly Gly Tyr Pro Ala Pro Lys Gly Ala Arg Asp Ile Tyr Ser Tyr Asp Ile Lys Gly Val Ala Gln Asn Ile Arg Leu Asn Leu Thr Asp Asn Arg Ser Thr Gly Gln Arg Leu Ala Asp Arg Phe His Asn Ala Gly Ser Met Leu Thr Gln Gly Val Gly Asp Gly Phe Lys Arg Ala Thr Arg Tyr Ser Pro Glu Leu Asp Arg Ser Gly Asn Ala Ala Glu Ala Phe Asn Gly Thr Ala Asp Ile Val Lys Asn Ile Ile Gly Ala Ala Gly Glu Ile Val Gly Ala Gly Asp Ala Val Gln Gly Ile Ser Glu Gly Ser Asn Ile Ala Val Met His Gly Leu Giy Leu Leu Ser Thr Glu Asn Lys Met Ala Arg Ile Asn Asp Leu Ala Asp Met Ala Gln Leu Lys Asp Tyr Ala Ala Ala Ala Ile Arg Asp Trp Ala Val Gln Asn Pro Asn Ala Ala Gln Gly Ile Glu Ala Val Ser Asn Ile Phe Met Ala Ala Ile Pro Ile Lys Gly Ile Gly Ala Val Arg Gly Lys Tyr Gly Leu Gly Gly Ile Thr Ala His Pro Ile Lys Arg Ser Gln Met Gly Ala Ile Ala Leu Pro Lys Gly Lys Ser Ala Val Ser Asp Asn Phe Ala Asp Ala Ala Tyr Ala Lys Tyr Pro Ser Pro Tyr His Ser Arg Asn Ile Arg Ser Asn Leu Glu Gln Arg Tyr Gly Lys Glu Asn Ile Thr Ser Ser Thr Val Pro Pro Ser Asn Gly Lys Asn Val Lys Leu Ala Asp Gln Arg His Pro Lys Thr Gly Val Pro Phe Asp Gly Lys Gly Phe Pro Asn Phe Glu Lys His Val Lys Tyr Asp Thr Leu Glu His His His His His His <210> 35 <211> 2019 <212> DNA
<213> Artificial Sequence <220>
<223> 0RF46.1-741 <400> 35 atgtcagatt tggcaaacga ttcttttatc cggcaggttc tcgaccgtca gcatttcgaa 60 cccgacggga aataccacct attcggcagc aggggggaac ttgccgagcg cagcggccat 120 atcggattgg gaaaaataca aagccatcag ttgggcaacc tgatgattca acaggcggcc 180 attaaaggaa atatcggcta cattgtccgc ttttccgatc acgggcacga agtccattcc 240 cccttcgaca accatgcctc acattccgat tctgatgaag ccggtagtcc cgttgacgga 300 tttagccttt accgcatcca ttgggacgga tacgaacacc atcccgccga cggctatgac 360 gggccacagg gcggcggcta tcccgctccc aaaggcgcga gggatatata cagctacgac 420 ataaaaggcg ttgcccaaaa tatccgcctc aacctgaccg acaaccgcag caccggacaa 480 cggcttgccg accgtttcca caatgccggt agtatgctga cgcaaggagt aggcgacgga 540 ttcaaacgcg ccacccgata cagccccgag ctggacagat cgggcaatgc cgccgaagcc 600 ttcaacggca ctgcagatat cgttaaaaac atcatcggcg cggcaggaga aattgtcggc 660 gcaggcgatg ccgtgcaggg cataagcgaa ggctcaaaca ttgctgtcat gcacggcttg 720 ggtctgcttt ccaccgaaaa caagatggcg cgcatcaacg atttggcaga tatggcgcaa 780 ctcaaagact atgccgcagc agccatccgc gattgggcag tccaaaaccc caatgccgca 840 caaggcatag aagccgtcag caatatcttt atggcagcca tccccatcaa agggattgga 900 gctgttcggg gaaaatacgg cttgggcggc atcacggcac atcctatcaa gcggtcgcag 960 atgggcgcga tcgcattgcc gaaagggaaa tccgccgtca gcgacaattt tgccgatgcg 1020 gcatacgcca aatacccgtc cccttaccat tcccgaaata tccgttcaaa cttggagcag 1080 cgttacggga aagaaaacat cacctcctca accgtgccgc cgtcaaacgg caaaaatgtc 1140 aaactggcag accaacgcca cccgaagaca ggcgtaccgt ttgacggtaa agggtttccg 1200 aattttgaga agcacgtgaa atatgatacg ggatccggag ggggtggtgt cgccgccgac 1260 atcggtgcgg ggcttgccga tgcactaacc gcaccgctcg accataaaga caaaggtttg 1320 cagtctttga cgctggatca gtccgtcagg aaaaacgaga aactgaagct ggcggcacaa 1380 ggtgcggaaa aaacttatgg aaacggtgac agcctcaata cgggcaaatt gaagaacgac 1440 aaggtcagcc gtttcgactt tatccgccaa atcgaagtgg acgggcagct cattaccttg 1500 gagagtggag agttccaagt atacaaacaa agccattccg ccttaaccgc ctttcagacc 1560 gagcaaatac aagattcgga gcattccggg aagatggttg cgaaacgcca gttcagaatc 1620 ggcgacatag cgggcgaaca tacatctttt gacaagcttc ccgaaggcgg cagggcgaca 1680 tatcgcggga cggcgttcgg ttcagacgat gccggcggaa aactgaccta caccatagat 1740 ttcgccgcca agcagggaaa cggcaaaatc gaacatttga aatcgccaga actcaatgtc 1800 gacctggccg ccgccgatat caagccggat ggaaaacgcc atgccgtcat cagcggttcc 1860 gtcctttaca accaagccga gaaaggcagt tactccctcg gtatctttgg cggaaaagcc 1920 caggaagttg ccggcagcgc ggaagtgaaa accgtaaacg gcatacgcca tatcggcctt 1980 gccgccaagc aactcgagca ccaccaccac caccactga 2019 <210> 36 <211> 672 <212> PRT
<213> Artificial Sequence <220>
<223> 0RF46.1-741 <400> 36 Met Ser Asp Leu Ala Asn Asp Ser Phe Ile Arg Gln Val Leu Asp Arg Gin His Phe Glu Pro Asp Gly Lys Tyr His Leu Phe Gly Ser Arg Gly Glu Leu Ala Glu Arg Ser Gly His Ile Gly Leu Gly Lys Ile Gln Ser His Gin Leu Gly Asn Leu Net Ile Gln Gin Ala Ala Ile Lys Gly Asn Ile Gly Tyr Ile Val Arg Phe Ser Asp His Gly His Glu Val His Ser Pro Phe Asp Asn His Ala Ser His Ser Asp Ser Asp Glu Ala Gly Ser Pro Val Asp Gly Phe Ser Leu Tyr Arg Ile His Trp Asp Gly Tyr Glu His His Pro Ala Asp Gly Tyr Asp Gly Pro Gln Gly Gly G1y Tyr Pro Ala Pro Lys Gly Ala Arg Asp Ile Tyr Ser Tyr Asp Ile Lys Gly Val Ala Gin Asn Ile Arg Leu Asn Leu Thr Asp Asn Arg Ser Thr Gly Gln Arg Leu Ala Asp Arg Phe His Asn Ala Gly Ser Met Leu Thr Gln Gly Val Gly Asp Gly Phe Lys Arg Ala Thr Arg Tyr Ser Pro Glu Leu Asp Arg Ser Gly Asn Ala Ala Glu Ala Phe Asn Gly Thr Ala Asp Ile Val Lys Asn Ile Ile Gly Ala Ala Gly Glu Ile Val Gly Ala Gly Asp Ala Val Gln Gly Ile Ser Glu Gly Ser Asn Ile Ala Val Met His Gly Leu Gly Leu Leu Ser Thr Glu Asn Lys Met Ala Arg Ile Asn Asp Leu Ala Asp Met Ala Gln Leu Lys Asp Tyr Ala Ala Ala Ala Ile Arg Asp Trp Ala Val Gln Asn Pro Asn Ala Ala Gln Gly Ile Glu Ala Val Ser Asn Ile Phe Met Ala Ala Ile Pro Ile Lys Gly Ile Gly Ala Val Arg Gly Lys Tyr Gly Leu Gly Gly Ile Thr Ala His Pro Ile Lys Arg Ser Gln Met Gly Ala Ile Ala Leu Pro Lys Gly Lys Ser Ala Val Ser Asp Asn Phe Ala Asp Ala Ala Tyr Ala Lys Tyr Pro Ser Pro Tyr His Ser Arg Asn Ile Arg Ser Asn Leu Glu Gln Arg Tyr Gly Lys Glu Asn Ile Thr Ser Ser Thr Val Pro Pro Ser Asn Gly Lys Asn Val Lys Leu Ala Asp Gln Arg His Pro Lys Thr Gly Val Pro Phe Asp Gly Lys Gly Phe Pro Asn Phe Glu Lys His Val Lys Tyr Asp Thr Gly Ser Gly Gly Gly Gly Val Ala Ala Asp Ile Gly Ala Gly Leu Ala Asp Ala Leu Thr Ala Pro Leu Asp His Lys Asp Lys Gly Leu Gln Ser Leu Thr Leu Asp Gln Ser Val Arg Lys Asn Glu Lys Leu Lys Leu Ala Ala Gln Gly Ala Glu Lys Thr Tyr Gly Asn Gly Asp Ser Leu Asn Thr Gly Lys Leu Lys Asn Asp Lys Val Ser Arg Phe Asp Phe Ile Arg Gln Ile Glu Val Asp Gly Gln Leu Ile Thr Leu Glu Ser Gly Glu Phe Gln Val Tyr Lys Gln Ser His Ser Ala Leu Thr Ala Phe Gln Thr Glu Gin Ile Gln Asp Ser Glu His Ser Gly Lys Met Val Ala Lys Arg Gln Phe Arg Ile Gly Asp Ile Ala Gly Glu His Thr Ser Phe Asp Lys Leu Pro Glu Gly Gly Arg Ala Thr Tyr Arg Gly Thr Ala Phe Gly Ser Asp Asp Ala Gly Gly Lys Leu Thr Tyr Thr Ile Asp Phe Ala Ala Lys Gln Gly Asn Gly Lys Ile Glu His Leu Lys Ser Pro Glu Leu Asn Val Asp Leu Ala Ala Ala Asp Ile Lys Pro Asp Gly Lys Arg His Ala Val Ile Ser Gly Ser Val Leu Tyr Asn Gln Ala Glu Lys Gly Ser Tyr Ser Leu Gly Ile Phe Gly Gly Lys Ala Gln Glu Val Ala Gly Ser Ala Glu Val Lys Thr Val Asn Gly Ile Arg His Ile Gly Leu Ala Ala Lys Gln Leu Glu His His His His His His <210> 37 <211> 2421 <212> DNA
<213> Artificial Sequence <220>
<223> ORF46.1-961 <400> 37 atgtcagatt tggcaaacga ttcttttatc cggcaggttc tcgaccgtca gcatttcgaa 60 cccgacggga aataccacct attcggcagc aggggggaac ttgccgagcg cagcggccat 120 atcggattgg gaaaaataca aagccatcag ttgggcaacc tgatgattca acaggcggcc 180 attaaaggaa atatcggcta cattgtccgc ttttccgatc acgggcacga agtccattcc 240 cccttcgaca accatgcctc acattccgat tctgatgaag ccggtagtcc cgttgacgga 300 tttagccttt accgcatcca ttgggacgga tacgaacacc atcccgccga cggctatgac 360 gggccacagg gcggcggcta tcccgctccc aaaggcgcga gggatatata cagctacgac 420 ataaaaggcg ttgcccaaaa tatccgcctc aacctgaccg acaaccgcag caccggacaa 480 cggcttgccg accgtttcca caatgccggt agtatgctga cgcaaggagt aggcgacgga 540 ttcaaacgcg ccacccgata cagccccgag ctggacagat cgggcaatgc cgccgaagcc 600 ttcaacggca ctgcagatat cgttaaaaac atcatcggcg cggcaggaga aattgtcggc 660 gcaggcgatg ccgtgcaggg cataagcgaa ggctcaaaca ttgctgtcat gcacggcttg 720 ggtctgcttt ccaccgaaaa caagatggcg cgcatcaacg atttggcaga tatggcgcaa 780 ctcaaagact atgccgcagc agccatccgc gattgggcag tccaaaaccc caatgccgca 840 caaggcatag aagccgtcag caatatcttt atggcagcca tccccatcaa agggattgga 900 gctgttcggg gaaaatacgg cttgggcggc atcacggcac atcctatcaa gcggtcgcag 960 atgggcgcga tcgcattgcc gaaagggaaa tccgccgtca gcgacaattt tgccgatgcg 1020 gcatacgcca aatacccgtc cccttaccat tcccgaaata tccgttcaaa cttggagcag 1080 cgttacggca aagaaaacat cacctcctca accgtgccgc cgtcaaacgg caaaaatgtc 1140 aaactggcag accaacgcca cccgaagaca ggcgtaccgt ttgacggtaa agggtttccg 1200 aattttgaga agcacgtgaa atatgatacg ggatccggag gaggaggagc cacaaacgac 1260 gacgatgtta aaaaagctgc cactgtggcc attgctgctg cctacaacaa tggccaagaa 1320 atcaacggtt tcaaagctgg agagaccatc tacgacattg atgaagacgg cacaattacc 1380 aaaaaagacg caactgcagc cgatgttgaa gccgacgact ttaaaggtct gggtctgaaa 1440 aaagtcgtga ctaacctgac caaaaccgtc aatgaaaaca aacaaaacgt cgatgccaaa 1500 gtaaaagctg cagaatctga aatagaaaag ttaacaacca agttagcaga cactgatgcc 1560 gctttagcag atactgatgc cgctctggat gcaaccacca acgccttgaa taaattggga 1620 gaaaatataa cgacatttgc tgaagagact aagacaaata tcgtaaaaat tgatgaaaaa 1680 ttagaagccg tggctgatac cgtcgacaag catcccgaag cattcaacga tatcgccgat 1740 tcattggatg aaaccaacac taaggcagac gaagccgtca aaaccgccaa tgaagccaaa 1800 cagacggccg aagaaaccaa acaaaacgtc gatgccaaag taaaagctgc agaaactgca 1860 gcaggcaaag ccgaagctgc cgctggcaca gctaatactg cacccgacaa ggccgaagct 1920 gtcgctgcaa aagttaccga catcaaagct gatatcgcta cgaacaaaga taatattgct 1980 aaaaaagcaa acagtgccga cgtgtacacc agagaagagt ctgacagcaa atttgtcaga 2040 attgatggtc tgaacgctac taccgaaaaa ttggacacac gcttggcttc tgctgaaaaa 2100 tccattgccg atcacgatac tcgcctgaac ggtttggata aaacagtgtc agacctgcgc 2160 aaagaaaccc gccaaggcct tgcagaacaa gccgcgctct ccggtctgtt ccaaccttac 2220 aacgtgggtc ggttcaatgt aacggctgca gtcggcggct acaaatccga atcggcagtc 2280 gccatcggta ccggcttccg ctttaccgaa aactttgccg ccaaagcagg cgtggcagtc 2340 ggcacttcgt ccggttcttc cccagcctac catgtcggcg tcaattacga gtggctcgag 2400 caccaccacc accaccactg a 2421 <210> 38 <211> 806 <212> PRT
<213> Artificial Sequence <220>
<223> ORF46.1-961 <400> 38 Met Ser Asp Leu Ala Asn Asp Ser Phe Ile Arg Gln Val Leu Asp Arg Gln His Phe Glu Pro Asp Gly Lys Tyr His Leu Phe Gly Ser Arg Gly Glu Leu Ala Glu Arg Ser Gly His Ile Gly Leu Gly Lys Ile Gln Ser His Gln Leu Gly Asn Leu Met Ile Gln Gln Ala Ala Ile Lys Gly Asn Ile Gly Tyr Ile Val Arg Phe Ser Asp His Gly His Glu Val His Ser Pro Phe Asp Asn His Ala Ser His Ser Asp Ser Asp Glu Ala Gly Ser Pro Val Asp Gly Phe Ser Leu Tyr Arg Ile His Trp Asp Gly Tyr Glu His His Pro Ala Asp Gly Tyr Asp Gly Pro Gln Gly Gly Gly Tyr Pro Ala Pro Lys Gly Ala Arg Asp Ile Tyr Ser Tyr Asp Ile Lys Gly Val Ala Gln Asn Ile Arg Leu Asn Leu Thr Asp Asn Arg Ser Thr Gly Gln Arg Leu Ala Asp Arg Phe His Asn Ala Gly Ser Met Leu Thr Gln Gly Val Gly Asp Gly Phe Lys Arg Ala Thr Arg Tyr Ser Pro Glu Leu Asp Arg Ser Gly Asn Ala Ala Glu Ala Phe Asn Gly Thr Ala Asp Ile Val Lys Asn Ile Ile Gly Ala Ala Gly Glu Ile Val Gly Ala Gly Asp Ala Val Gln Gly Ile Ser Glu Gly Ser Asn Ile Ala Val Met His Gly Leu Gly Leu Leu Ser Thr Glu Asn Lys Met Ala Arg Ile Asn Asp Leu Ala Asp Met Ala Gln Leu Lys Asp Tyr Ala Ala Ala Ala Ile Arg Asp Trp Ala Val Gln Asn Pro Asn Ala Ala Gln Gly Ile Glu Ala Val Ser Asn Ile Phe Met Ala Ala Ile Pro Ile Lys Gly Ile Gly Ala Val Arg Gly Lys Tyr Gly Leu Gly Gly Ile Thr Ala His Pro Ile Lys Arg Ser Gln Met Gly Ala Ile Ala Leu Pro Lys Gly Lys Ser Ala Val Ser Asp Asn Phe Ala Asp Ala Ala Tyr Ala Lys Tyr Pro Ser Pro Tyr His Ser Arg Asn Ile Arg Ser Asn Leu Glu Gln Arg Tyr Gly Lys Glu Asn Ile Thr Ser Ser Thr Val Pro Pro Ser Asn Gly Lys Asn Val Lys Leu Ala Asp Gln Arg His Pro Lys Thr Gly Val Pro Phe Asp Gly Lys Gly Phe Pro Asn Phe Glu Lys His Val Lys Tyr Asp Thr Gly Ser Gly Gly Gly Gly Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile Ala Ala Ala Tyr Asn Asn Gly Gln Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gln Thr Ala Glu Glu Thr Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gln Gly Leu Ala Glu Gln Ala Ala Leu Ser Gly Leu Phe Gln Pro Tyr Asn Val Gly Arg Phe Asn Val Thr Ala Ala Val Gly Gly Tyr Lys Ser Glu Ser Ala Val Ala Ile Gly Thr Gly Phe Arg Phe Thr Glu Asn Phe Ala Ala Lys Ala Gly Val Ala Val Gly Thr Ser Ser Gly Ser Ser Ala Ala Tyr His Val Gly Val Asn Tyr Glu Trp Leu Glu His His His His His His <210> 39 <211> 2256 <212> DNA
<213> Artificial Sequence <220>
<223> 0RF46.1-961c <400> 39 atgtcagatt tggcaaacga ttcttttatc cggcaggttc tcgaccgtca gcatttcgaa 60 cccgacggga aataccacct attcggcagc aggggggaac ttgccgagcg cagcggccat 120 atcggattgg gaaaaataca aagccatcag ttgggcaacc tgatgattca acaggcggcc 180 attaaaggaa atatcggcta cattgtccgc ttttccgatc acgggcacga agtccattcc 240 cccttcgaca accatgcctc acattccgat tctgatgaag ccggtagtcc cgttgacgga 300 tttagccttt accgcatcca ttgggacgga tacgaacacc atcccgccga cggctatgac 360 gggccacagg gcggcggcta tcccgctccc aaaggcgcga gggatatata cagctacgac 420 ataaaaggcg ttgcccaaaa tatccgcctc aacctgaccg acaaccgcag caccggacaa 480 cggcttgccg accgtttcca caatgccggt agtatgctga cgcaaggagt aggcgacgga 540 ttcaaacgcg ccacccgata cagccccgag ctggacagat cgggcaatgc cgccgaagcc 600 ttcaacggca ctgcagatat cgttaaaaac atcatcggcg cggcaggaga aattgtcggc 660 gcaggcgatg ccgtgcaggg cataagcgaa ggctcaaaca ttgctgtcat gcacggcttg 720 ggtctgcttt ccaccgaaaa caagatggcg cgcatcaacg atttggcaga tatggcgcaa 780 ctcaaagact atgccgcagc agccatccgc gattgggcag tccaaaaccc caatgccgca 840 caaggcatag aagccgtcag caatatcttt atggcagcca tccccatcaa agggattgga 900 gctgttcggg gaaaatacgg cttgggcggc atcacggcac atcctatcaa gcggtcgcag 960 atgggcgcga tcgcattgcc gaaagggaaa tccgccgtca gcgacaattt tgccgatgcg 1020 gcatacgcca aatacccgtc cccttaccat tcccgaaata tccgttcaaa cttggagcag 1080 cgttacggca aagaaaacat cacctcctca accgtgccgc cgtcaaacgg caaaaatgtc 1140 aaactggcag accaacgcca cccgaagaca ggcgtaccgt ttgacggtaa agggtttccg 1200 aattttgaga agcacgtgaa atatgatacg ggatccggag gaggaggagc cacaaacgac 1260 gacgatgtta aaaaagctgc cactgtggcc attgctgctg cctacaacaa tggccaagaa 1320 atcaacggtt tcaaagctgg agagaccatc tacgacattg atgaagacgg cacaattacc 1380 aaaaaagacg caactgcagc cgatgttgaa gccgacgact ttaaaggtct gggtctgaaa 1440 aaagtcgtga ctaacctgac caaaaccgtc aatgaaaaca aacaaaacgt cgatgccaaa 1500 gtaaaagctg cagaatctga aatagaaaag ttaacaacca agttagcaga cactgatgcc 1560 gctttagcag atactgatgc cgctctggat gcaaccacca acgccttgaa taaattggga 1620 gaaaatataa cgacatttgc tgaagagact aagacaaata tcgtaaaaat tgatgaaaaa 1680 ttagaagccg tggctgatac cgtcgacaag catgccgaag cattcaacga tatcgccgat 1740 tcattggatg aaaccaacac taaggcagac gaagccgtca aaaccgccaa tgaagccaaa 1800 cagacggccg aagaaaccaa acaaaacgtc gatgccaaag taaaagctgc agaaactgca 1860 gcaggcaaag ccgaagctgc cgctggcaca gctaatactg cagccgacaa ggccgaagct 1920 gtcgctgcaa aagttaccga catcaaagct gatatcgcta cgaacaaaga taatattgct 1980 aaaaaagcaa acagtgccga cgtgtacacc agagaagagt ctgacagcaa atttgtcaga 2040 attgatggtc tgaacgctac taccgaaaaa ttggacacac gcttggcttc tgctgaaaaa 2100 tccattgccg atcacgatac tcgcctgaac ggtttggata aaacagtgtc agacctgcgc 2160 aaagaaaccc gccaaggcct tgcagaacaa gccgccctct ccggtctgtt ccaaccttac 2220 aacgtgggtc tcgagcacca ccaccaccac cactga 2256 <210> 40 <211> 751 <212> PRT
<213> Artificial Sequence <220>
<223> ORF46.1-961c <400> 40 Met Ser Asp Leu Ala Asn Asp Ser Phe Ile Arg Gln Val Leu Asp Arg Gln His Phe Glu Pro Asp Gly Lys Tyr His Leu Phe Gly Ser Arg Gly Glu Leu Ala Glu Arg Ser Gly His Ile Gly Leu Gly Lys Ile Gln Ser His Gln Leu Gly Asn Leu Met Ile Gln Gln Ala Ala Ile Lys Gly Asn Ile Gly Tyr Ile Val Arg Phe Ser Asp His Gly His Glu Val His Ser Pro Phe Asp Asn His Ala Ser His Ser Asp Ser Asp Glu Ala Gly Ser Pro Val Asp Gly Phe Ser Leu Tyr Arg Ile His Trp Asp Gly Tyr Glu His His Pro Ala Asp Gly Tyr Asp Gly Pro Gln Gly Gly Gly Tyr Pro Ala Pro Lys Gly Ala Arg Asp Ile Tyr Ser Tyr Asp Ile Lys Gly Val Ala Gln Asn Ile Arg Leu Asn Leu Thr Asp Asn Arg Ser Thr Gly Gln Arg Leu Ala Asp Arg Phe His Asn Ala Gly Ser Met Leu Thr Gln Gly Val Gly Asp Gly Phe Lys Arg Ala Thr Arg Tyr Ser Pro Glu Leu Asp Arg Ser Gly Asn Ala Ala Glu Ala Phe Asn Gly Thr Ala Asp Ile Val Lys Asn Ile Ile Gly Ala Ala Gly Glu Ile Val Gly Ala Gly Asp Ala Val Gln Gly Ile Ser Glu Gly Ser Asn Ile Ala Val Met His Gly Leu Gly Leu Leu Ser Thr Glu Asn Lys Met Ala Arg Ile Asn Asp Leu Ala Asp Met Ala Gln Leu Lys Asp Tyr Ala Ala Ala Ala Ile Arg Asp Trp Ala Val Gln Asn Pro Asn Ala Ala Gln Gly Ile Glu Ala Val Ser Asn Ile Phe Met Ala Ala Ile Pro Ile Lys Gly Ile Gly Ala Val Arg Gly Lys Tyr Gly Leu Gly Gly Ile Thr Ala His Pro Ile Lys Arg Ser Gln Met Gly Ala Ile Ala Leu Pro Lys Gly Lys Ser Ala Val Ser Asp Asn Phe Ala Asp Ala Ala Tyr Ala Lys Tyr Pro Ser Pro Tyr His Ser Arg Asn Ile Arg Ser Asn Leu Glu Gln Arg Tyr Gly Lys Glu Asn Ile Thr Ser Ser Thr Val Pro Pro Ser Asn Gly Lys Asn Val Lys Leu Ala Asp Gln Arg His Pro Lys Thr Gly Val Pro Phe Asp Gly Lys Gly Phe Pro Asn Phe Glu Lys His Val Lys Tyr Asp Thr Giy Ser Gly Gly Gly Gly Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile Ala Ala Ala Tyr Asn Asn Gly Gln Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gln Thr Ala Glu Glu Thr Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gln Gly Leu Ala Glu Gln Ala Ala Leu Ser Gly Leu Phe Gln Pro Tyr Asn Val Gly Leu Glu His His His His His His <210> 41 <211> 2421 <212> DNA
<213> Artificial Sequence <220>
<223> 961-0RF46.1 <400> 41 atggccacaa acgacgacga tgttaaaaaa gctgccactg tggccattgc tgctgcctac 60 aacaatggcc aagaaatcaa cggtttcaaa gctggagaga ccatctacga cattgatgaa 120 gacggcacaa ttaccaaaaa agacgcaact gcagccgatg ttgaagccga cgactttaaa 180 ggtctgggtc tgaaaaaagt cgtgactaac ctgaccaaaa ccgtcaatga aaacaaacaa 240 aacgtcgatg ccaaagtaaa agctgcagaa tctgaaatag aaaagttaac aaccaagtta 300 gcagacactg atgccgcttt agcagatact gatgccgctc tggatgcaac caccaacgcc 360 ttgaataaat tgggagaaaa tataacgaca tttgctgaag agactaagac aaatatcgta 420 aaaattgatg aaaaattaga agccgtggct gataccgtcg acaagcatgc cgaagcattc 480 aacgatatcg ccgattcatt ggatgaaacc aacactaagg cagacgaagc cgtcaaaacc 540 gccaatgaag ccaaacagac ggccgaagaa accaaacaaa acgtcgatgc caaagtaaaa 600 gctgcagaaa ctgcagcagg caaagccgaa gctgccgctg gcacagctaa tactgcagcc 660 gacaaggccg aagctgtcgc tgcaaaagtt accgacatca aagctgatat cgctacgaac 720 aaagataata ttgctaaaaa agcaaacagt gccgacgtgt acaccagaga agagtctgac 780 agcaaatttg tcagaattga tggtctgaac gctactaccg aaaaattgga cacacgcttg 840 gcttctgctg aaaaatccat tgccgatcac gatactcgcc tgaacggttt ggataaaaca 900 gtgtcagacc tgcgcaaaga aacccgccaa ggccttgcag aacaagccgc gctctccggt 960 ctgttccaac cttacaacgt gggtcggttc aatgtaacgg ctgcagtcgg cggctacaaa 1020 tccgaatcgg cagtcgccat cggtaccggc ttccgcttta ccgaaaactt tgccgccaaa 1080 gcaggcgtgg cagtcggcac ttcgtccggt tcttccgcag cctaccatgt cggcgtcaat 1140 tacgagtggg gatccggagg aggaggatca gatttggcaa acgattcttt tatccggcag 1200 gttctcgacc gtcagcattt cgaacccgac gggaaatacc acctattcgg cagcaggggg 1260 gaacttgccg agcgcagcgg ccatatcgga ttgggaaaaa tacaaagcca tcagttgggc 1320 aacctgatga ttcaacaggc ggccattaaa ggaaatatcg gctacattgt ccgcttttcc 1380 gatcacgggc acgaagtcca ttcccccttc gacaaccatg cctcacattc cgattctgat 1440 gaagccggta gtcccgttga cggatttagc ctttaccgca tccattggga cggatacgaa 1500 caccatcccg ccgacggcta tgacgggcca cagggcggcg gctatcccgc tcccaaaggc 1560 gcgagggata tatacagcta cgacataaaa ggcgttgccc aaaatatccg cctcaacctg 1620 accgacaacc gcagcaccgg acaacggctt gccgaccgtt tccacaatgc cggtagtatg 1680 ctgacgcaag gagtaggcga cggattcaaa cgcgccaccc gatacagccc cgagctggac 1740 agatcgggca atgccgccga agccttcaac ggcactgcag atatcgttaa aaacatcatc 1800 ggcgcggcag gagaaattgt cggcgcaggc gatgccgtgc agggcataag cgaaggctca 1860 aacattgctg tcatgcacgg cttgggtctg ctttccaccg aaaacaagat ggcgcgcatc 1920 aacgatttgg cagatatggc gcaactcaaa gactatgccg cagcagccat ccgcgattgg 1980 gcagtccaaa accccaatgc cgcacaaggc atagaagccg tcagcaatat ctttatggca 2040 gccatcccca tcaaagggat tggagctgtt cggggaaaat acggcttggg cggcatcacg 2100 gcacatccta tcaagcggtc gcagatgggc gcgatcgcat tcccaaaagg gaaatccgcc 2160 gtcagcgaca attttgccga tgcggcatac gccaaatacc cgtcccctta ccattcccga 2220 aatatccgtt caaacttgga gcagcgttac ggcaaagaaa acatcacctc ctcaaccgtg 2280 ccgccgtcaa acggcaaaaa tgtcaaactg gcagaccaac gccacccgaa gacaggcgta 2340 ccgtttgacg gtaaagggtt tccgaatttt gagaagcacg tgaaatatga tacgctcgag 2400 caccaccacc accaccactg a 2421 <210> 42 <211> 806 <212> PRT
<213> Artificial Sequence <220>
<223> 961-ORF46.1 <400> 42 Net Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile Ala Ala Ala Tyr Asn Asn Gly Gln Glu Ile An Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gin Thr Ala Glu Glu Thr Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gln Gly Leu Ala Glu Gln Ala Ala Leu Ser Gly Leu Phe Gln Pro Tyr Asn Val Gly Arg Phe Asn Val Thr Ala Ala Val Gly Gly Tyr Lys Ser Glu Ser Ala Val Ala Ile Gly Thr Gly Phe Arg Phe Thr Glu Asn Phe Ala Ala Lys Ala Gly Val Ala Val Gly Thr Ser Ser Gly Ser Ser Ala Ala Tyr His Val Gly Val Asn Tyr Glu Trp Gly Ser Gly Gly Gly Gly Ser Asp Leu Ala Asn Asp Ser Phe Ile Arg Gln Val Leu Asp Arg Gln His Phe Glu Pro Asp Gly Lys Tyr His Leu Phe Gly Ser Arg Gly Glu Leu Ala Glu Arg Ser Gly His Ile Gly Leu Gly Lys Ile Gln Ser His Gln Leu Gly Asn Leu Met Ile Gln Gin Ala Ala Ile Lys Gly Asn Ile Gly Tyr Ile Val Arg Phe Ser Asp His Gly His Glu Val His Ser Pro Phe Asp Asn His Ala Ser His Ser Asp Ser Asp Glu Ala Gly Ser Pro Val Asp Gly Phe Ser Leu Tyr Arg Ile His Trp Asp Gly Tyr Glu His His Pro Ala Asp Gly Tyr Asp Gly Pro Gln Gly Gly Gly Tyr Pro Ala Pro Lys Gly Ala Arg Asp Ile Tyr Ser Tyr Asp Ile Lys Gly Val Ala Gln Asn Ile Arg Leu Asn Leu Thr Asp Asn Arg Ser Thr Gly Gln Arg Leu Ala Asp Arg Phe His Asn Ala Gly Ser Met Leu Thr Gln Gly Val Gly Asp Gly Phe Lys Arg Ala Thr Arg Tyr Ser Pro Glu Leu Asp Arg Ser Gly Asn Ala Ala Glu Ala Phe Asn Gly Thr Ala Asp Ile Val Lys Asn Ile Ile Gly Ala Ala Gly Glu Ile Val Gly Ala Gly Asp Ala Val Gln Gly Ile Ser Glu Gly Ser Asn Ile Ala Val Met His Gly Leu Gly Leu Leu Ser Thr Glu Asn Lys Met Ala Arg Ile Asn Asp Leu Ala Asp Met Ala Gln Leu Lys Asp Tyr Ala Ala Ala Ala Ile Arg Asp Trp Ala Val Gln Asn Pro Asn Ala Ala Gln Gly Ile Glu Ala Val Ser Asn Ile Phe Met Ala Ala Ile Pro Ile Lys Gly Ile Gly Ala Val Arg Gly Lys Tyr Gly Leu Gly Gly Ile Thr Ala His Pro Ile Lys Arg Ser Gln Met Gly Ala Ile Ala Leu Pro Lys Gly Lys Ser Ala Val Ser Asp Asn Phe Ala Asp Ala Ala Tyr Ala Lys Tyr Pro Ser Pro Tyr His Ser Arg Asn Ile Arg Ser Asn Leu Glu Gln Arg Tyr Gly Lys Glu Asn Ile Thr Ser Ser Thr Val Pro Pro Ser Asn Gly Lys Asn Val Lys Leu Ala Asp Gln Arg His Pro Lys Thr Gly Val Pro Phe Asp Gly Lys Gly Phe Pro Asn Phe Glu Lys His Val Lys Tyr Asp Thr Leu Glu His His His His His His <210> 43 <211> 1938 <212> DNA
<213> Artificial Sequence <220>
<223> 961-741 <400> 43 atggccacaa acgacgacga tgttaaaaaa gctgccactg tggccattgc tgctgcctac 60 aacaatggcc aagaaatcaa cggtttcaaa gctggagaga ccatctacga cattgatgaa 120 gacggcacaa ttaccaaaaa agacgcaact gcagccgatg ttgaagccga cgactttaaa 180 ggtctgggtc tgaaaaaagt cgtgactaac ctgaccaaaa ccgtcaatga aaacaaacaa 240 aacgtcgatg ccaaagtaaa agctgcagaa tctgaaatag aaaagttaac aaccaagtta 300 gcagacactg atgccgcttt agcagatact gatgccgctc tggatgcaac caccaacgcc 360 ttgaataaat tgggagaaaa tataacgaca tttgctgaag agactaagac aaatatcgta 420 aaaattgatg aaaaattaga agccgtggct gataccgtcg acaagcatgc cgaagcattc 480 aacgatatcg ccgattcatt ggatgaaacc aacactaagg cagacgaagc cgtcaaaacc 540 gccaatgaag ccaaacagac ggccgaagaa accaaacaaa acgtcgatgc caaagtaaaa 600 gctgcagaaa ctgcagcagg caaagccgaa gctgccgctg gcacagctaa tactgcagcc 660 gacaaggccg aagctgtcgc tgcaaaagtt accgacatca aagctgatat cgctacgaac 720 aaagataata ttgctaaaaa agcaaacagt gccgacgtgt acaccagaga agagtctgac 780 agcaaatttg tcagaattga tggtctgaac gctactaccg aaaaattgga cacacgcttg 840 gcttctgctg aaaaatccat tgccgatcac gatactcgcc tgaacggttt ggataaaaca 900 gtgtcagacc tgcgcaaaga aacccgccaa ggccttgcag aacaagccgc gctctccggt 960 ctgttccaac cttacaacgt gggtcggttc aatgtaacgg ctgcagtcgg cggctacaaa 1020 tccgaatcgg cagtcgccat cggtaccggc ttccgcttta ccgaaaactt tgccgccaaa 1080 gcaggcgtgg cagtcggcac ttcgtccggt tcttccgcag cctaccatgt cggcgtcaat 1140 tacgagtggg gatccggagg gggtggtgtc gccgccgaca tcggtgcggg gcttgccgat 1200 gcactaaccg caccgctcga ccataaagac aaaggtttgc agtctttgac gctggatcag 1260 tccgtcagga aaaacgagaa actgaagctg gcggcacaag gtgcggaaaa aacttatgga 1320 aacggtgaca gcctcaatac gggcaaattg aagaacgaca aggtcagccg tttcgacttt 1380 atccgccaaa tcgaagtgga cgggcagctc attaccttgg agagtggaga gttccaagta 1440 tacaaacaaa gccattccgc cttaaccgcc tttcagaccg agcaaataca agattcggag 1500 cattccggga agatggttgc gaaacgccag ttcagaatcg gcgacatagc gggcgaacat 1560 acatcttttg acaagcttcc cgaaggcggc agggcgacat atcgcgggac ggcgttcggt 1620 tcagacgatg ccggcggaaa actgacctac accatagatt tcgccgccaa gcagggaaac 1680 ggcaaaatcg aacatttgaa atcgccagaa ctcaatgtcg acctggccgc cgccgatatc 1740 aagccggatg gaaaacgcca tgccgtcatc agcggttccg tcctttacaa ccaagccgag 1800 aaaggcagtt actccctcgg tatctttggc ggaaaagccc aggaagttgc cggcagcgcg 1860 gaagtgaaaa ccgtaaacgg catacgccat atcggccttg ccgccaagca actcgagcac 1920 caccaccacc accactga 1938 <210> 44 <211> 645 <212> PRT
<213> Artificial Sequence <220>
<223> 961-741 <400> 44 Met Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile Ala Ala Ala Tyr Asn Asn Gly Gln Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gin Thr Ala Glu Glu Thr Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gln Gly Leu Ala G1u Gln Ala Ala Leu Ser Gly Leu Phe Gln Pro Tyr Asn Val Gly Arg Phe Asn Val Thr Ala Ala Val Gly Gly Tyr Lys Ser Glu Ser Ala Val Ala Ile Gly Thr Gly Phe Arg Phe Thr Glu Asn Phe Ala Ala Lys Ala Gly Val Ala Val Gly Thr Ser Ser Gly Ser Ser Ala Ala Tyr His Val Gly Val Asn Tyr Glu Trp Gly Ser Gly Gly Gly Gly Val Ala Ala Asp Ile Gly Ala Gly Leu Ala Asp Ala Leu Thr Ala Pro Leu Asp His Lys Asp Lys Gly Leu Gln Ser Leu Thr Leu Asp Gln Ser Val Arg Lys Asn Glu Lys Leu Lys Leu Ala Ala Gln Gly Ala Glu Lys Thr Tyr Gly Asn GI.y Asp Ser Leu Asn Thr Gly Lys Leu Lys Asn Asp Lys Val Ser Arg Phe Asp Phe Ile Arg Gln Ile Glu Val Asp Gly Gln Leu Ile Thr Leu G]u Ser Gly Glu Phe Gln Val Tyr Lys Gln Ser His Ser Ala Leu Thr Ala Phe Gln Thr Glu Gln Ile Gln Asp Ser Glu His Ser Gly Lys Met Val Ala Lys Arg Gln Phe Arg Ile Gly Asp Ile Ala Gly Glu His Thr Ser Phe Asp Lys Leu Pro Glu Gly Gly Arg Ala Thr Tyr Arg Gly Thr Ala Phe Gly Ser Asp Asp Ala Giy Gly Lys Leu Thr Tyr Thr Ile Asp Phe Ala Ala Lys Gln Gly Asn Gly Lys Ile Glu His Leu Lys Ser Pro Glu Leu Asn Val Asp Leu Ala Ala Ala Asp Ile Lys Pro Asp Gly Lys Arg His Ala Val Ile Ser Gly Ser Val Leu Tyr Asn Gln Ala Glu Lys Gly Ser Tyr Ser Leu Gly Ile Phe Gly Gly Lys Ala Gln Glu Val Ala GLy Ser Ala Glu Val Lys Thr Val Asn Gly Ile Arg His Ile Gly Leu Ala Ala Lys Gin Leu Glu His His His His His His <210> 45 <211> 4335 <212> DNA
<213> Artificial Sequence <220>
<223> 961-983 <400> 45 atggccacaa acgacgacga tgttaaaaaa gctgccactg tggccattgc tgctgcctac 60 aacaatggcc aagaaatcaa cggtttcaaa gctggagaga ccatctacga cattgatgaa 120 gacggcacaa ttaccaaaaa agacgcaact gcagccgatg ttgaagccga cgactttaaa 180 ggtctgggtc tgaaaaaagt cgtgactaac ctgaccaaaa ccgtcaatga aaacaaacaa 240 aacgtcgatg ccaaagtaaa agctgcagaa tctgaaatag aaaagttaac aaccaagtta 300 gcagacactg atgccgcttt agcagatact gatgccgctc tggatgcaac caccaacgcc 360 ttgaataaat tgggagaaaa tataacgaca tttgctgaag agactaagac aaatatcgta 420 aaaattgatg aaaaattaga agccgtggct gataccgtcg acaagcatgc cgaagcattc 480 aacgatatcg ccgattcatt ggatgaaacc aacactaagg cagacgaagc cgtcaaaacc 540 gccaatgaag ccaaacagac ggccgaagaa accaaacaaa acgtcgatgc caaagtaaaa 600 gctgcagaaa ctgcagcagg caaagccgaa gctgccgctg gcacagctaa tactgcagcc 660 gacaaggccg aagctgtcgc tgcaaaagtt accgacatca aagctgatat cgctacgaac 720 aaagataata ttgctaaaaa agcaaacagt gccgacgtgt acaccagaga agagtctgac 780 agaaaatttg tcagaattga tggtctgaac gctactaccg aaaaattgga cacacgcttg 840 gcttctgctg aaaaatccat tgccgatcac gatactcgcc tgaacggttt ggataaaaca 900 gtgtcagacc tgcgcaaaga aacccgccaa ggccttgcag aacaagccgc gctctccggt 960 ctgttccaac cttacaacgt gggtcggttc aatgtaacgg ctgcagtcgg cggctacaaa 1020 tccgaatcgg cagtcgccat cggtaccggc ttccgcttta ccgaaaactt tgccgccaaa 1080 gcaggcgtgg cagtcggcac ttcgtccggt tcttccgcag cctaccatgt cggcgtcaat 1140 tacgagtggg gatccggcgg aggcggcact tctgcgcccg acttcaatgc aggcggtacc 1200 ggtatcggca gcaacagcag agcaacaaca gcgaaatcag cagcagtatc ttacgccggt 1260 atcaagaacg aaatgtgcaa agacagaagc atgctctgtg ccggtcggga tgacgttgcg 1320 gttacagaca gggatgccaa aatcaatgcc ccccccccga atctgcatac cggagacttt 1380 ccaaacccaa atgacgcata caagaatttg atcaacctca aacctgcaat tgaagcaggc 1440 tatacaggac gcggggtaga ggtaggtatc gtcgacacag gcgaatccgt cggcagcata 1500 tcctttcccg aactgtatgg cagaaaagaa cacggctata acgaaaatta caaaaactat 1560 acggcgtata tgcggaagga agcgcctgaa gacggaggcg gtaaagacat tgaagcttct 1620 ttcgacgatg aggccgttat agagactgaa gcaaagccga cggatatccg ccacgtaaaa 1680 gaaatcggac acatcgattt ggtctcccat attattggcg ggcgttccgt ggacggcaga 1740 cctgcaggcg gtattgcgcc cgatgcgacg ctacacataa tgaatacgaa tgatgaaacc 1800 aagaacgaaa tgatggttgc agccatccgc aatgcatggg tcaagctggg cgaacgtggc 1860 gtgcgcatcg tcaataacag ttttggaaca acatcgaggg caggcactgc cgaccttttc 1920 caaatagcca attcggagga gcagtaccgc caagcgttgc tcgactattc cggcggtgat 1980 aaaacagacg agggtatccg cctgatgcaa cagagcgatt acggcaacct gtcctaccac 2040 atccgtaata aaaacatgct tttcatcttt tcgacaggca atgacgcaca agctcagccc 2100 aacacatatg ccctattgcc attttatgaa aaagacgctc aaaaaggcat tatcacagtc 2160 gcaggcgtag accgcagtgg agaaaagttc aaacgggaaa tgtatggaga accgggtaca 2220 gaaccgcttg agtatggctc caaccattgc ggaattactg ccatgtggtg cctgtcggca 2280 ccctatgaag caagcgtccg tttcacccgt acaaacccga ttcaaattgc cggaacatcc 2340 ttttccgcac ccatcgtaac cggcacggcg gctctgctgc tgcagaaata cccgtggatg 2400 agtaacgaca acctgcgtac cacgttgctg acgacggctc aggacatcgg tgcagtcggc 2460 gtggacagca agttcggctg gggactgctg gatgcgggta aggccatgaa cggacccgcg 2520 tcctttccgt tcggcgactt taccgccgat acgaaaggta catccgatat tgcctactcc 2580 ttccgtaacg acatttcagg cacgggcggc ctgatcaaaa aaggcggcag ccaactgcaa 2640 ctgcacggca acaacaccta tacgggcaaa accattatcg aaggcggttc gctggtgttg 2700 tacggcaaca acaaatcgga tatgcgcgtc gaaaccaaag gtgcgctgat ttataacggg 2760 gcggcatccg gcggcagcct gaacagcgac ggcattgtct atctggcaga taccgaccaa 2820 tccggcgcaa acgaaaccgt acacatcaaa ggcagtctgc agctggacgg caaaggtacg 2880 ctgtacacac gtttgggcaa actgctgaaa gtggacggta cggcgattat cggcggcaag 2940 ctgtacatgt cggcacgcgg caagggggca ggctatctca acagtaccgg acgacgtgtt 3000 cccttcctga gtgccgccaa aatcgggcag gattattctt tcttcacaaa catcgaaacc 3060 gacggcggcc tgctggcttc cctcgacagc gtcgaaaaaa cagcgggcag tgaaggcgac 3120 acgctgtcct attatgtccg tcgcggcaat gcggcacgga ctgcttcggc agcggcacat 3180 tccgcgcccg ccggtctgaa acacgccgta gaacagggcg gcagcaatct ggaaaacctg 3240 atggtcgaac tggatgcctc cgaatcatcc gcaacacccg agacggttga aactgcggca 3300 gccgaccgca cagatatgcc gggcatccgc ccctacggcg caactttccg cgcagcggca 3360 gccgtacagc atgcgaatgc cgccgacggt gtacgcatct tcaacagtct cgccgctacc 3420 gtctatgccg acagtaccgc cgcccatgcc gatatgcagg gacgccgcct gaaagccgta 3480 tcggacgggt tggaccacaa cggcacgggt ctgcgcgtca tcgcgcaaac ccaacaggac 3540 ggtggaacgt gggaacaggg cggtgttgaa ggcaaaatgc gcggcagtac ccaaaccgtc 3600 ggcattgccg cgaaaaccgg cgaaaatacg acagcagccg ccacactggg catgggacgc 3660 agcacatgga gcgaaaacag tgcaaatgca aaaaccgaca gcattagtct gtttgcaggc 3720 atacggcacg atgcgggcga tatcggctat ctcaaaggcc tgttctccta cggacgctac 3780 aaaaacagca tcagccgcag caccggtgcg gacgaacatg cggaaggcag cgtcaacggc 3840 acgctgatgc agctgggcgc actgggcggt gtcaacgttc cgtttgccgc aacgggagat 3900 ttgacggtcg aaggcggtct gcgctacgac ctgctcaaac aggatgcatt cgccgaaaaa 3960 ggcagtgctt tgggctggag cggcaacagc ctcactgaag gcacgctggt cggactcgcg 4020 ggtctgaagc tgtcgcaacc cttgagcgat aaagccgtcc tgtttgcaac ggcgggcgtg 4080 gaacgcgacc tgaacggacg cgactacacg gtaacgggcg gctttaccgg cgcgactgca 4140 gcaaccggca agacgggggc acgcaatatg caccacaacc gtctggttgc cggcctgggc 4200 gcggatgtcg aattcggcaa cggctggaac ggcttggcac gttacagcta cgccggttcc 4260 aaacagtacg gcaaccacag cggacgagtc ggcgtaggct accggttcct cgagcaccac 4320 caccaccacc actga 4335 <210> 46 <211> 1444 <212> PRT
<213> Artificial Sequence <220>
<223> 961-983 <400> 46 Met Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile Ala Ala Ala Tyr Asn Asn Gly Gln Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gln Thr Ala Glu Glu Thr Lys Gin Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gin Gly Leu Ala Glu Gin Ala Ala Leu Ser Gly Leu Phe Gin Pro Tyr Asn Val Gly Arg Phe Asn Val Thr Ala Ala Val Gly Gly Tyr Lys Ser Glu Ser Ala Val Ala Ile Gly Thr Gly Phe Arg Phe Thr Glu Asn Phe Ala Ala Lys Ala Gly Val Ala Val Gly Thr Ser Ser Gly Ser Ser Ala Ala Tyr His Val Gly Val Asn Tyr Glu Trp Gly Ser Gly Gly Gly Gly Thr Ser Ala Pro Asp Phe Asn Ala Gly Gly Thr Gly Ile Gly Ser Asn Ser Arg Ala Thr Thr Ala Lys Ser Ala Ala Val Ser Tyr Ala Gly Ile Lys Asn Glu Met Cys Lys Asp Arg Ser Met Leu Cys Ala Gly Arg Asp Asp Val Ala Val Thr Asp Arg Asp Ala Lys Ile Asn Ala Pro Pro Pro Asn Leu His Thr Gly Asp Phe Pro Asn Pro Asn Asp Ala Tyr Lys Asn Leu Ile Asn Leu Lys Pro Ala Ile Glu Ala Gly Tyr Thr Gly Arg Gly Val Glu Val Gly Ile Val Asp Thr Gly Glu Ser Val Gly Ser Ile Ser Phe Pro Glu Leu Tyr Gly Arg Lys Glu His Gly Tyr Asn Glu Asn Tyr Lys Asn Tyr Thr Ala Tyr Met Arg Lys Glu Ala Pro Glu Asp Gly Gly Gly Lys Asp Ile Glu Ala Ser Phe Asp Asp Glu Ala Val Ile Glu Thr Glu Ala Lys Pro Thr Asp Ile Arg His Val Lys Glu Ile Gly His Ile Asp Leu Val Ser His Ile Ile Gly Gly Arg Ser Val Asp Gly Arg Pro Ala Gly Gly Ile Ala Pro Asp Ala Thr Leu His Ile Met Asn Thr Asn Asp Glu Thr Lys Asn Glu Met Met Val Ala Ala Ile Arg Asn Ala Trp Val Lys Leu Gly G1u Arg Gly Val Arg Ile Val Asn Asn Ser Phe Gly Thr Thr Ser Arg Ala Gly Thr Ala Asp Leu Phe Gln Ile Ala Asn Ser Glu Glu Gln Tyr Arg Gln Ala Leu Leu Asp Tyr Ser Gly Gly Asp Lys Thr Asp Glu Gly Ile Arg Leu Met Gln Gin Ser Asp Tyr Gly Asn Leu Ser Tyr His Ile Arg Asn Lys Asn Met Leu Phe Ile Phe Ser Thr Gly Asn Asp Ala Gln Ala Gln Pro Asn Thr Tyr Ala Leu Leu Pro Phe Tyr Glu Lys Asp Ala Gln Lys Gly Ile Ile Thr Val 705 710 71.5 720 Ala Gly Val Asp Arg Ser Gly Glu Lys Phe Lys Arg Glu Met Tyr Gly Glu Pro Gly Thr Glu Pro Leu Glu Tyr Gly Ser Asn His Cys Gly Ile Thr Ala Met Trp Cys Leu Ser Ala Pro Tyr Glu Ala Ser Val Arg Phe Thr Arg Thr Asn Pro Ile Gln Ile Ala Gly Thr Ser Phe Ser Ala Pro Ile Val Thr Gly Thr Ala Ala Leu Leu Leu Gln Lys Tyr Pro Trp Met Ser Asn Asp Asn Leu Arg Thr Thr Leu Leu Thr Thr Ala Gln Asp Ile Gly Ala Val Gly Val Asp Ser Lys Phe Gly Trp Gly Leu Leu Asp Ala Gly Lys Ala Met Asn Gly Pro Ala Ser Phe Pro Phe Gly Asp Phe Thr Ala Asp Thr Lys Gly Thr Ser Asp Ile Ala Tyr Ser Phe Arg Asn Asp Ile Ser Gly Thr Gly Gly Leu Ile Lys Lys Gly Gly Ser Gln Leu Gln Leu His Gly Asn Asn Thr Tyr Thr Gly Lys Thr. Ile Ile Glu Gly Gly Ser Leu Val Leu Tyr Gly Asn Asn Lys Ser Asp Met Arg Val Glu Thr Lys Gly Ala Leu Ile Tyr Asn Gly Ala Ala Ser Gly Gly Ser Leu Asn Ser Asp Gly Ile Val Tyr Leu Ala Asp Thr Asp Gln Ser Gly Ala Asn Glu Thr Val His Ile Lys Gly Ser Leu Gln Leu Asp Gly Lys Gly Thr Leu Tyr Thr Arg Leu Gly Lys Leu Leu Lys Val Asp Gly Thr Ala Ile Ile Gly Gly Lys Leu Tyr Met Ser Ala Arg Gly Lys Gly Ala Gly Tyr Leu Asn Ser Thr Gly Arg Arg Val Pro Phe Leu Ser Ala Ala Lys Ile Gly Gln Asp Tyr Ser Phe Phe Thr Asn Ile Glu Thr Asp Gly Gly Leu Leu Ala Ser Leu Asp Ser Val Glu Lys Thr Ala Gly Ser Glu Gly Asp Thr Leu Ser Tyr Tyr Val Arg Arg Gly Asn Ala Ala Arg Thr Ala Ser Ala Ala Ala His Ser Ala Pro Ala Gly Leu Lys His Ala Val Glu Gln Gly Gly Ser Asn Leu Glu Asn Leu Met Val Glu Leu Asp Ala Ser Glu Ser Ser Ala Thr Pro Glu Thr Val Glu Thr Ala Ala Ala Asp Arg Thr Asp Met Pro Gly Ile Arg Pro Tyr Gly Ala Thr Phe Arg Ala Ala Ala Ala Val Gin His Ala Asn Ala Ala Asp Gly Val Arg Ile Phe Asn Ser Leu Ala Ala Thr Val Tyr Ala Asp Ser Thr Ala Ala His Ala Asp Met Gin Gly Arg Arg Leu Lys Ala Val Ser Asp Gly Leu Asp His Asn Gly Thr Gly Leu Arg Val Ile Ala Gin Thr Gin Gin Asp Gly Gly Thr Trp Glu Gin Gly Gly Val Glu Gly Lys Met Arg Gly Ser Thr Gin Thr Val Gly Ile Ala Ala Lys Thr Gly Glu Asn Thr Thr Ala Ala Ala Thr Leu Gly Met Gly Arg Ser Thr Trp Ser Glu Asn Ser Ala Asn Ala Lys Thr Asp Ser Ile Ser Leu Phe Ala Gly Ile Arg His Asp Ala Gly Asp Ile Gly Tyr Leu Lys Gly Leu Phe Ser Tyr Gly Arg Tyr Lys Asn Ser Ile Ser Arg Ser Thr Gly Ala Asp Glu His Ala Glu Gly Ser Val Asn Gly Thr Leu Met Gin Leu Gly Ala Leu Gly Gly Val Asn Val Pro Phe Ala Ala Thr Gly Asp Leu Thr Val Glu Gly Gly Leu Arg Tyr Asp Leu Leu Lys Gln Asp Ala Phe Ala Glu Lys Gly Ser Ala Leu Gly Trp Ser Gly Asn Ser Leu Thr Glu Gly Thr Leu Val Gly Leu Ala Gly Leu Lys Leu Ser Gln Pro Leu Ser Asp Lys Ala Val Leu Phe Ala Thr Ala Gly Val Glu Arg Asp Leu Asn Gly Arg Asp Tyr Thr Val Thr Gly Gly Phe Thr Gly Ala Thr Ala Ala Thr Gly Lys Thr Gly Ala Arg Asn Met. Pro His Thr Arg Leu Val Ala Gly Leu Gly Ala Asp Val Glu Phe Gly Asn Gly Trp Asn Gly Leu Ala Arg Tyr Ser Tyr Ala Gly Ser Lys Gln Tyr Gly Asn His Ser Gly Arg Val Gly Val Gly Tyr Arg Phe Leu Glu His His His His His His <210> 47 <211> 2256 <212> DNA
<213> Artificial Sequence <220>
<223> 961c-0RF46.1 <400> 47 atggccacaa acgacgacga tgttaaaaaa gctgccactg tggccattgc tgctgcctac 60 aacaatggcc aagaaatcaa cggtttcaaa gctggagaga ccatctacga cattgatgaa 120 gacggcacaa ttaccaaaaa agacgcaact gcagccgatg ttgaagccga cgactttaaa 180 ggtctgggtc tgaaaaaagt cgtgactaac ctgaccaaaa ccgtcaatga aaacaaacaa 240 aacgtcgatg ccaaagtaaa agctgcagaa tctgaaatag aaaagttaac aaccaagtta 300 gcagacactg atgccgcttt agcagatact gatgccgctc tggatgcaac caccaacgcc 360 ttgaataaat tgggagaaaa tataacgaca tttgctgaag agactaagac aaatatcgta 420 aaaattgatg aaaaattaga agccgtggct gataccgtcg acaagcatgc cgaagcattc 480 aacgatatcg ccgattcatt ggatgaaacc aacactaagg cagacgaagc cgtcaaaacc 540 gccaatgaag ccaaacagac ggccgaagaa accaaacaaa acgtcgatgc caaagtaaaa 600 gctgcagaaa ctgcagcagg caaagccgaa gctgccgctg gcacagctaa tactgcagcc 660 gacaaggccg aagctgtcgc tgcaaaagtt accgacatca aagctgatat cgctacgaac 720 aaagataata ttgctaaaaa agcaaacagt gccgacgtgt acaccagaga agagtctgac 780 agcaaatttg tcagaattga tggtctgaac gctactaccg aaaaattgga cacacgcttg 840 gcttctgctg aaaaatccat tgccgatcac gatactcgcc tgaacggttt ggataaaaca 900 gtgtcagacc tgcgcaaaga aacccgccaa ggccttgcag aacaagccgc gctctccggt 960 ctgttccaac cttacaacgt gggtggatcc ggaggaggag gatcagattt ggcaaacgat 1020 tcttttatcc ggcaggttct cgaccgtcag catttcgaac ccgacgggaa ataccaccta 1080 ttcggcagca ggggggaact tgccgagcgc agcggccata tcggattggg aaaaatacaa 1140 agccatcagt tgggcaacct gatgattcaa caggcggcca ttaaaggaaa tatcggctac 1200 attgtccgct tttccgatca cgggcacgaa gtccattccc ccttcgacaa ccatgcctca 1260 cattccgatt ctgatgaagc cggtagtccc gttgacggat ttagccttta ccgcatccat 1320 tgggacggat acgaacacca tcccgccgac ggctatgacg ggccacaggg cggcggctat 1380 cccgctccca aaggcgcgag ggatatatac agctacgaca taaaaggcgt tgcccaaaat 1440 atccgcctca acctgaccga caaccgcagc accggacaac ggcttgccga ccgtttccac 1500 aatgccggta gtatgctgac gcaaggagta ggcgacggat tcaaacgcgc cacccgatac 1560 agccccgagc tggacagatc gggcaatgcc gccgaagcct tcaacggcac tgcagatatc 1620 gttaaaaaca tcatcggcgc ggcaggagaa attgtcggcg caggcgatgc cgtgcagggc 1680 ataagcgaag gctcaaacat tgctgtcatg cacggcttgg gtctgctttc caccgaaaac 1740 aagatggcgc gcatcaacga tttggcagat atggcgcaac tcaaagacta tgccgcagca 1800 gccatccgcg attgggcagt ccaaaacccc aatgccgcac aaggcataga agccgtcagc 1860 aatatcttta tggcagccat ccccatcaaa gggattggag ctgttcgggg aaaatacggc 1920 ttgggcggca tcacggcaca tcctatcaag cggtcgcaga tgggcgcgat cgcattgccg 1980 aaagggaaat ccgccgtcag cgacaatttt gccgatgcgg catacgccaa atacccgtcc 2040 ccttaccatt cccgaaatat ccgttcaaac ttggagcagc gttacggcaa agaaaacatc 2100 acctcctcaa ccgtgccgcc gtcaaacggc aaaaatgtca aactggcaga ccaacgccac 2160 ccgaagacag gcgtaccgtt tgacggtaaa gggtttccga attttgagaa gcacgtgaaa 2220 tatgatacgc tcgagcacca ccaccaccac cactga 2256 <210> 48 <211> 751 <212> PRT
<213> Artificial Sequence <220>
<223> 961c-ORF46.1 <400> 48 Met Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile Ala Ala Ala Tyr Asn Asn Gly Gln Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gln Thr Ala Glu Glu Thr Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gln Gly Leu Ala Glu Gln Ala Ala Leu Ser Gly Leu Phe Gln Pro Tyr Asn Val Gly Gly Ser Gly Gly Gly Gly Ser Asp Leu Ala Asn Asp Ser Phe Ile Arg Gln Val Leu Asp Arg Gln His Phe Glu Pro Asp Gly Lys Tyr His Leu Phe Gly Ser Arg Gly Glu Leu Ala Glu Arg Ser Gly His Ile Gly Leu Gly Lys Ile Gln Ser His Gln Leu Gly Asn Leu Met Ile Gln Gln Ala Ala Ile Lys Gly Asn Ile Gly Tyr Ile Val Arg Phe Ser Asp His Gly His Glu Val His Ser Pro Phe Asp Asn His Ala Ser His Ser Asp Ser Asp Glu Ala Gly Ser Pro Val Asp Gly Phe Ser Leu Tyr Arg Ile His Trp Asp Gly Tyr Glu His His Pro Ala Asp Gly Tyr Asp Gly Pro Gln Gly Gly Gly Tyr Pro Ala Pro Lys Gly Ala Arg Asp Ile Tyr Ser Tyr Asp Ile Lys Gly Val Ala Gln Asn Ile Arg Leu Asn Leu Thr Asp Asn Arg Ser Thr Gly Gln Arg Leu Ala Asp Arg Phe His Asn Ala Gly Ser Met Leu Thr Gln Gly Val Gly Asp Gly Phe Lys Arg Ala Thr Arg Tyr Ser Pro Glu Leu Asp Arg Ser Gly Asn Ala Ala Glu Ala Phe Asn Gly Thr Ala Asp Ile Val Lys Asn Ile Ile Gly Ala Ala Gly Glu Ile Val Gly Ala Gly Asp Ala Val Gln Gly Ile Ser Glu Gly Ser Asn Ile Ala Val Met His Gly Leu Gly Leu Leu Ser Thr Glu Asn Lys Met Ala Arg Ile Asn Asp Leu Ala Asp Met Ala Gln Leu Lys Asp Tyr Ala Ala Ala Ala Ile Arg Asp Trp Ala Val Gln Asn Pro Asn Ala Ala Gin Gly Ile Glu Ala Val Ser Asn Ile Phe Met Ala Ala Ile Pro Ile Lys Gly Ile Gly Ala Val Arg Gly Lys Tyr Gly Leu Gly Gly Ile Thr Ala His Pro Ile Lys Arg Ser Gln Met Gly Ala Ile Ala Leu Pro Lys Gly Lys Ser Ala Val Ser Asp Asn Phe Ala Asp Ala Ala Tyr Ala Lys Tyr Pro Ser Pro Tyr His Ser Arg Asn Ile Arg Ser Asn Leu Glu Gln Arg Tyr Gly Lys Glu Asn Ile Thr Ser Ser Thr Val Pro Pro Ser Asn Gly Lys Asn Val Lys Leu Ala Asp Gln Arg His Pro Lys Thr Gly Val Pro Phe Asp Gly Lys Gly Phe Pro Asn Phe Glu Lys His Val Lys Tyr Asp Thr Leu Glu His His His His His His <210> 49 <211> 1773 <212> DNA
<213> Artificial Sequence <220>
<223> 961c-741 <400> 49 atggccacaa acgacgacga tgttaaaaaa gctgccactg tggccattgc tgctgcctac 60 aacaatggcc aagaaatcaa cggtttcaaa gctggagaga ccatctacga cattgatgaa 120 gacggcacaa ttaccaaaaa agaagcaact gcagccgatg ttgaagccga cgactttaaa 180 ggtctgggtc tgaaaaaagt cgtgactaac ctgaccaaaa ccgtcaatga aaacaaacaa 240 aacgtcgatg ccaaagtaaa agctgcagaa tctgaaatag aaaagttaac aaccaagtta 300 gcagacactg atgccgcttt agcagatact gatgccgctc tggatgcaac caccaacgcc 360 ttgaataaat tgggagaaaa tataacgaca tttgctgaag agactaagac aaatatcgta 420 aaaattgatg aaaaattaga agccgtggct gataccgtcg acaagcatgc cgaagcattc 480 aacgatatcg ccgattcatt ggatgaaacc aacactaagg cagacgaagc cgtcaaaacc 540 gccaatgaag ccaaacagac ggccgaagaa accaaacaaa acgtcgatgc caaagtaaaa 600 gctgcagaaa ctgcagcagg caaagccgaa gctgccgctg gcacagctaa tactgcagcc 660 gacaaggccg aagctgtcgc tgcaaaagtt accgacatca aagctgatat cgctacgaac 720 aaagataata ttgttaaaaa agcaaacagt gccgacgtgt acaccagaga agagtctgac 780 agcaaatttg tcagaattga tggtctgaac gctactaccg aaaaattgga cacacgcttg 840 gcttctgctg aaaaatccat tgccgatcac gatactcgcc tgaacggttt ggataaaaca 900 gtgtcagacc tgcgcaaaga aacccgccaa ggccttgcag aacaagccgc gctctccggt 960 ctgttccaac cttacaacgt gggtggatcc ggagggggtg gtgtcgccgc cgacatcggt 1020 gcggggcttg ccgatgcact aaccgcaccg ctcgaccata aagacaaagg tttgcagtct 1080 ttgacgctgg atcagtccgt caggaaaaac gagaaactga agctggcggc acaaggtgcg 1140 gaaaaaactt atggaaacgg tgacagcctc aatacgggca aattgaagaa cgacaaggtc 1200 agccgtttcg actttatccg ccaaatcgaa gtggacgggc agctcattac cttggagagt 1260 ggagagttcc aagtatacaa acaaagccat tccgccttaa ccgcctttca gaccgagcaa 1320 atacaagatt cggagcattc cgggaagatg gttgcgaaac gccagttcag aatcggcgac 1380 atagcgggcg aacatacatc ttttgacaag cttcccgaag gcggcagggc gacatatcgc 1440 gggacggcgt tcggttcaga cgatgccggc ggaaaactga cctacaccat agatttcgcc 1500 gccaagcagg gaaacggcaa aatcgaacat ttgaaatcgc cagaactcaa tgtcgacctg 1560 gccgccgccg atatcaagcc ggatggaaaa cgccatgccg tcatcagcgg ttccgtcctt 1620 tacaaccaag ccgagaaagg cagttactcc ctcggtatct ttggcggaaa agcccaggaa 1680 gttgccggca gcgcggaagt gaaaaccgta aacggcatac gccatatcgg ccttgccgcc 1740 aagcaactcg agcaccacca ccaccaccac tga 1773 <210> 50 <211> 590 <212> PRT
<213> Artificial Sequence <220>
<223> 961c-741 <400> 50 Met Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile Ala Ala Ala Tyr Asn Asn Gly Gln Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gln Thr Ala Glu Glu Thr Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gln Gly Leu Ala Glu Gln Ala Ala Leu Ser Gly Leu Phe Gln Pro Tyr Asn Val Gly Gly Ser Gly Gly Gly Gly Val Ala Ala Asp Ile Gly Ala Gly Leu Ala Asp Ala Leu Thr Ala Pro Leu Asp His Lys Asp Lys Gly Leu Gln Ser Leu Thr Leu Asp Gln Ser Val Arg Lys Asn Glu Lys Leu Lys Leu Ala Ala Gln Gly Ala Glu Lys Thr Tyr Gly Asn Gly Asp Ser Leu Asn Thr Gly Lys Leu Lys Asn Asp Lys Val Ser Arg Phe Asp Phe Ile Arg Gln Ile Glu Val Asp Gly Gln Leu Ile Thr Leu Glu Ser Gly Glu Phe Gln Val Tyr Lys Gln Ser His Ser Ala Leu Thr Ala Phe Gln Thr Glu Gln Ile Gln Asp Ser Glu His Ser Gly Lys Met Val Ala Lys Arg Gln Phe Arg Ile Gly Asp Ile Ala Gly Glu His Thr Ser Phe Asp Lys Leu Pro Glu Gly Gly Arg Ala Thr Tyr Arg Gly Thr Ala Phe Gly Ser Asp Asp Ala Gly Gly Lys Leu Thr Tyr Thr Ile Asp Phe Ala Ala Lys Gln Gly Asn Gly Lys Ile Glu His Leu Lys Ser Pro Glu Leu Asn Val Asp Leu Ala Ala Ala Asp Ile Lys Pro Asp Gly Lys Arg His Ala Val Ile Ser Gly Ser Val Leu Tyr Asn Gln Ala Glu Lys Gly Ser Tyr Ser Leu Gly Ile Phe Gly Gly Lys Ala Gln Glu Val Ala Gly Ser Ala Glu Val Lys Thr Val Asn Gly Ile Arg His Ile Gly Leu Ala Ala Lys Gln Leu Glu His His His His His His <210> 51 <211> 4170 <212> DNA
<213> Artificial Sequence <220>
<223> 961c-983 <400> 51 atggccacaa acgacgacga tgttaaaaaa gctgccactg tggccattgc tgctgcctac 60 aacaatggcc aagaaatcaa cggtttcaaa gctggagaga ccatctacga cattgatgaa 120 gacggcacaa ttaccaaaaa agacgcaact gcagccgatg ttgaagccga cgactttaaa 180 ggtctgggtc tgaaaaaagt cgtgactaac ctgaccaaaa ccgtcaatga aaacaaacaa 240 aacgtcgatg ccaaagtaaa agctgcagaa tctgaaatag aaaagttaac aaccaagtta 300 gcagacactg atgccgcttt agcagatact gatgccgctc tggatgcaac caccaacgcc 360 ttgaataaat tgggagaaaa tataacgaca tttgctgaag agactaagac aaatatcgta 420 aaaattgatg aaaaattaga agccgtggct gataccgtcg acaagcatgc cgaagcattc 480 aacgatatcg ccgattcatt ggataaaacc aacactaagg cagacgaagc cgtcaaaacc 540 gccaatgaag ccaaacagac ggccgaagaa accaaacaaa acgtcgatgc caaagtaaaa 600 gctgcagaaa ctgcagcagg caaagccgaa gctgccgctg gcacagctaa tactgcagcc 660 gacaaggccg aagctgtcgc tgcaaaagtt accgacatca aagctgatat cgctacgaac 720 aaagataata ttgctaaaaa agcaaacagt gccgacgtgt acaccagaga agagtctgac 780 agcaaatttg tcagaattga tggtctgaac gctactaccg aaaaattgga cacacgcttg 840 gcttctgctg aaaaatccat tgccgatcac gatactcgcc tgaacggttt ggataaaaca 900 gtgtcagacc tgcgcaaaga aacccgccaa ggccttgcag aacaatccgc gctctccggt 960 ctgttccaac cttacaacgt gggtggatcc ggcggaggcg gcacttctgc gcccgacttc 1020 aatgcaggcg gtaccggtat cggcagcaac agcagagcaa caacagcgaa atcagcagca 1080 gtatcttacg ccggtatcaa gaacgaaatg tgcaaagaca gaagcatgct ctgtgccggt 1140 cgggatgacg ttgcggttac agacagggat gccaaaatca atgccccccc cccgaatctg 1200 cataccggag actttccaaa cccaaatgac gcatacaaga atttgatcaa cctcaaacct 1260 gcaattgaag caggctatac aggacgcggg gtagaggtag gtatcgtcga cacaggcgaa 1320 tccgtcggca gcatatcctt tcccgaactg tatggcagaa aagaacacgg ctataacgaa 1380 aattacaaaa actatacggc gtatatgcgg aaggaagcgc ctgaagacgg aggcggtaaa 1440 gacattgaag cttctttcga cgatgaggcc gttatagaga ctgaagcaaa gccgacggat 1500 atccgccacg taaaagaaat cggacacatc gatttggtct cccatattat tggcgggcgt 1560 tccgtggacg gcagacctgc aggcggtatt gcgcccgatg cgacgctaca cataatgaat 1620 acgaatgatg aaaccaagaa cgaaatgatg gttgcagcca tccgcaatgc atgggtcaag 1680 ctgggcgaac gtggcgtgcg catcgtcaat aacagttttg gaacaacatc gagggcaggc 1740 actgccgacc ttttccaaat agccaattcg gaggagcagt accgccaagc gttgctcgac 1800 tattccggcg gtgataaaac agacgagggt atccgcctga tgcaacagag cgattacggc 1860 aacctgtcct accacatccg taataaaaac atgcttttca tcttttcgac aggcaatgac 1920 gcacaagctc agcccaacac atatgcccta ttgccatttt atgaaaaaga cgctcaaaaa 1980 ggcattatca cagtcgcagg cgtagaccgc agtggagaaa agttcaaacg ggaaatgtat 2040 ggagaaccgg gtacagaacc gcttgagtat ggctccaacc attgcggaat tactgccatg 2100 tggtgcctgt cggcacccta tgaagcaagc gtccgtttca cccgtacaaa cccgattcaa 2160 attgccggaa catccttttc cgcacccatc gtaaccggca cggcggctct gctgctgcag 2220 aaatacccgt ggatgagcaa cgacaacctg cgtaccacgt tgctgacgac ggctcaggac 2280 atcggtgcag tcggcgtgga cagcaagttc ggctggggac tgctggatgc gggtaaggcc 2340 atgaacggac ccgcgtcctt tccgttcggc gactttaccg ccgatacgaa aggtacatcc 2400 gatattgcct actccttccg taacgacatt tcaggcacgg gcggcctgat caaaaaaggc 2460 ggcagccaac tgcaactgca cggcaacaac acctatacgg gcaaaaccat tatcgaaggc 2520 ggttcgctgg tgttgtacgg caacaacaaa tcggatatgc gcgtcgaaac caaaggtgcg 2580 ctgatttata acggggcggc atccggcggc agcctgaaca gcgacggcat tgtctatctg 2640 gcagataccg accaatccgg cgcaaacgaa accgtacaca tcaaaggcag tctgcagctg 2700 gacggcaaag gtacgctgta cacacgtttg ggcaaactgc tgaaagtgga cggtacggcg 2760 attatcggcg gcaagctgta catgtcggca cgcggcaagg gggcaggcta tctcaacagt 2820 accggacgac gtgttccctt cctgagtgcc gccaaaatcg ggcaggatta ttctttcttc 2880 acaaacatcg aaaccgacgg cggcctgctg gcttccctcg acagcgtcga aaaaacagcg 2940 ggcagtgaag gcgacacgct gtcctattat gtccgtcgcg gcaatgcggc acgaactgct 3000 tcggcagcgg cacattccgc gcccgccggt ctgaaacacg ccgtagaaca gggcggcagc 3060 aatctggaaa acctgatggt cgaactggat gcctccgaat catccgcaac acccgagacg 3120 gttgaaactg cggcagccga ccgcacagat atgccgggca tccgccccta cggcgcaact 3180 ttccgcgcag cggcagccgt acagcatgcq aatgccgccg acggtgtacg catcttcaac 3240 agtctcgccg ctaccgtcta tgccgacagt accgccgccc atgccgatat gcagggacgc 3300 cgcctgaaag ccgtatcgga cgggttggac cacaacggca cgggtctgcg cgtcatcgcg 3360 caaacccaac aggacggtgg aacgtgggaa cagggcggtg ttgaaggcaa aatgcgcggc 3420 agtacccaaa ccgtcggcat tgccgcgaaa accggcgaaa atacgacagc agccgccaca 3480 ctgggcatgg gacgcagcac atggagcgaa aacagtgcaa atgcaaaaac cgacagcatt 3540 agtctgtttg caggcatacg gcacgatgcg ggcgatatcg gctatctcaa aggcctgttc 3600 tcctacggac gctacaaaaa cagcatcagc cgcagcaccg gtgcggacga acatgcggaa 3660 ggcagcgtca acggcacgct gatgcagctg ggcgcactgg gcggtgtcaa cgttccgttt 3720 gccgcaacgg gagatttgac ggtcgaaggc ggtctgcgct acgacctgct caaacaggat 3780 gcattcgccg aaaaaggcag tgctttgggc tggagcggca acagcctcac tgaaggcacg 3840 ctggtcggac tcgcgggtct gaagctgtcg caacccttga gcgataaagc cgtcctgttt 3900 gcaacggcgg gcgtggaacg cgacctgaac ggacgcgact acacggtaac gggcggcttt 3960 accggcgcga ctgcagcaac cggcaagacg ggggcacgca atatgccgca cacccgtctg 4020 gttgccggcc tgggcgcgga tgtcgaattc ggcaacggct ggaacggctt ggcacgttac 4080 agctacgccg gttccaaaca gtacggcaac cacagcggac gagtcggcgt aggctaccgg 4140 ttcctcgagc accaccacca ccaccactga 4170 <210> 52 <211> 1389 <212> PRT
<213> Artificial Sequence <220>
<223> 961c-983 <400> 52 Met Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile Ala Ala Ala Tyr Asn Asn Gly Gln Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gln Thr Ala Glu Glu Thr Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gln Gly Leu Ala Glu Gln Ala Ala Leu Ser Gly Leu Phe Gln Pro Tyr Asn Val Gly Gly Ser Gly Gly Gly Gly Thr Ser Ala Pro Asp Phe Asn Ala Gly Gly Thr Gly Ile Gly Ser Asn Ser Arg Ala Thr Thr Ala Lys Ser Ala Ala Val Ser Tyr Ala Gly Ile Lys Asn Glu Met Cys Lys Asp Arg Ser Met Leu Cys Ala Gly Arg Asp Asp Val Ala Val Thr Asp Arg Asp Ala Lys Ile Asn Ala Pro Pro Pro Asn Leu His Thr Gly Asp Phe Pro Asn Pro Asn Asp Ala Tyr Lys Asn Leu Ile Asn Leu Lys Pro Ala Ile Glu Ala Gly Tyr Thr Gly Arg Gly Val Glu Val Gly Ile Val Asp Thr Gly Glu Ser Val Gly Ser Ile Ser Phe Pro Glu Leu Tyr Gly Arg Lys Glu His Gly Tyr Asn Glu Asn Tyr Lys Asn Tyr Thr Ala Tyr Met Arg Lys Glu Ala Pro Glu Asp Gly Gly Gly Lys Asp Ile Glu Ala Ser Phe Asp Asp Glu Ala Val Ile Glu Thr Glu Ala Lys Pro Thr Asp Ile Arg His Val Lys Glu Ile Gly His Ile Asp Leu Val Ser His Ile Ile Gly Gly Arg Ser Val Asp Gly Arg Pro Ala Gly Gly Ile Ala Pro Asp Ala Thr Leu His Ile Met Asn Thr Asn Asp Glu Thr Lys Asn Glu Met Met Val Ala Ala Ile Arg Asn Ala Trp Val Lys Leu Gly Glu Arg Gly Val Arg Ile Val Asn Asn Ser Phe Gly Thr Thr Ser Arg Ala Gly Thr Ala Asp Leu Phe Gln Ile Ala Asn Ser Glu Glu Gln Tyr Arg Gln Ala Leu Leu Asp Tyr Ser Gly Gly Asp Lys Thr Asp Glu Gly Ile Arg Leu Met Gln Gln Ser Asp Tyr Gly Asn Leu Ser Tyr His Ile Arg Asn Lys Asn Met Leu Phe Ile Phe Ser Thr Gly Asn Asp Ala Gln Ala Gln Pro Asn Thr Tyr Ala Leu Leu Pro Phe Tyr Glu Lys Asp Ala Gln Lys Gly Ile Ile Thr Val Ala Gly Val Asp Arg Ser Gly Glu Lys Phe Lys Arg Glu Met Tyr Gly Glu Pro Gly Thr Glu Pro Leu Glu Tyr Gly Ser Asn His Cys Gly Ile Thr Ala Met Trp Cys Leu Ser Ala Pro Tyr Glu Ala Ser Val Arg Phe Thr Arg Thr Asn Pro Ile Gln Ile Ala Gly Thr Ser Phe Ser Ala Pro Ile Val Thr Gly Thr Ala Ala Leu Leu Leu Gln Lys Tyr Pro Trp Met Ser Asn Asp Asn Leu Arg Thr Thr Leu Leu Thr Thr Ala Gln Asp Ile Gly Ala Val Gly Val Asp Ser Lys Phe Gly Trp Gly Leu Leu Asp Ala Gly Lys Ala Met Asn Gly Pro Ala Ser Phe Pro Phe Gly Asp Phe Thr Ala Asp Thr Lys Gly Thr Ser Asp Ile Ala Tyr Ser Phe Arg Asn Asp Ile Ser Gly Thr Gly Gly Leu Ile Lys Lys Gly Gly Ser Gln Leu Gln Leu His Gly Asn Asn Thr Tyr Thr Gly Lys Thr Ile Ile Glu Gly Gly Ser Leu Val Leu Tyr Gly Asn Asn Lys Ser Asp Met Arg Val Glu Thr Lys Gly Ala Leu Ile Tyr Asn Gly Ala Ala Ser Gly Gly Ser Leu Asn Ser Asp Gly Ile Val Tyr Leu Ala Asp Thr Asp Gln Ser Gly Ala Asn Glu Thr Val His Ile Lys Gly Ser Leu Gln Leu Asp Gly Lys Gly Thr Leu Tyr Thr Arg Leu Gly Lys Leu Leu Lys Val Asp Gly Thr Ala Ile Ile Gly Gly Lys Leu Tyr Met Ser Ala Arg Gly Lys Gly Ala Gly Tyr Leu Asn Ser Thr Gly Arg Arg Val Pro Phe Leu Ser Ala Ala Lys Ile Gly Gln Asp Tyr Ser Phe Phe Thr Asn Ile Glu Thr Asp Gly Gly Leu Leu Ala Ser Leu Asp Ser Val Glu Lys Thr Ala Gly Ser Glu Gly Asp Thr Leu Ser Tyr Tyr Val Arg Arg Gly Asn Ala Ala Arg Thr Ala Ser Ala Ala Ala His Ser Ala Pro Ala Gly Leu Lys His Ala Val Glu Gln Gly Gly Ser Asn Leu Glu Asn Leu Met Val Glu Leu Asp Ala Ser Glu Ser Ser Ala Thr Pro Glu Thr Val Glu Thr Ala Ala Ala Asp Arg Thr Asp Met Pro Gly Ile Arg Pro Tyr Gly Ala Thr Phe Arg Ala Ala Ala Ala Val Gln His Ala Asn Ala Ala Asp Gly Val Arg Ile Phe Asn Ser Leu Ala Ala Thr Val Tyr Ala Asp Ser Thr Ala Ala His Ala Asp Met Gln Gly Arg Arg Leu Lys Ala Val Ser Asp Gly Leu Asp His Asn Gly Thr Gly Leu Arg Val Ile Ala Gln Thr Gln Gln Asp Gly Gly Thr Trp Glu Gln Gly Gly Val Glu Gly Lys Met Arg Gly Ser Thr Gln Thr Val Gly Ile Ala Ala Lys Thr Gly Glu Asn Thr Thr Ala Ala Ala Thr Leu Gly Met Gly Arg Ser Thr Trp Ser Glu Asn Ser Ala Asn Ala Lys Thr Asp Ser Ile Ser Leu Phe Ala Gly Ile Arg His Asp Ala Gly Asp Ile Gly Tyr Leu Lys Gly Leu Phe Ser Tyr Gly Arg Tyr Lys Asn Ser Ile Ser Arg Ser Thr Gly Ala Asp Glu His Ala Glu Gly Ser Val Asn Gly Thr Leu Met Gln Leu Gly Ala Leu Gly Gly Val Asn Val Pro Phe Ala Ala Thr Gly Asp Leu Thr Val Glu Gly Gly Leu Arg Tyr Asp Leu Leu Lys Gln Asp Ala Phe Ala Glu Lys Gly Ser Ala Leu Gly Trp Ser Gly Asn Ser Leu Thr Glu Gly Thr Leu Val Gly Leu Ala Gly Leu Lys Leu Ser Gln Pro Leu Ser Asp Lys Ala Val Leu Phe Ala Thr Ala Gly Val Glu Arg Asp Leu Asn Gly Arg Asp Tyr Thr Val Thr Gly Gly Phe Thr Gly Ala Thr Ala Ala Thr Gly Lys Thr Gly Ala Arg Asn Met Pro His Thr Arg Leu Val Ala Gly Leu Gly Ala Asp Val Glu Phe Gly Asn Gly Trp Asn Gly Leu Ala Arg Tyr Ser Tyr Ala Gly Ser Lys Gln Tyr Gly Asn His Ser Gly Arg Val Gly Val Gly Tyr Arg Phe Leu Glu His His His His His His <210> 53 <211> 2304 <212> DNA
<213> Artificial sequence <220>
<223> 961cL-ORF46.1 <400> 53 atgaaacact ttccatccaa agtactgacc acagccatcc ttgccacttt ctgtagcggc 60 gcactggcag ccacaaacga cgacgatgtt aaaaaagctg ccactgtggc cattgctgct 120 gcctacaaca atggccaaga aatcaacggt ttcaaagctg gagagaccat ctacgacatt 180 gatgaagacg gcacaattac caaaaaagac gcaactgcag ccgatgttga agccgacgac 240 tttaaaggtc tgggtctgaa aaaagtcgtg actaacctga ccaaaaccgt caatgaaaac 300 aaacaaaacg tcgatgccaa agtaaaagct gccgaatctg aaatagaaaa gttaacaacc 360 aagttagcag acactgatgc cgctttagca gatactgatg ccgctctgga tgcaaccacc 420 aacgccttga ataaattggg agaaaatata acgacatttg ctgaagagac taagacaaat 480 atcgtaaaaa ttgatgaaaa attagaagcc gtggctgata ccgtcgacaa gcatgccgaa 540 gcattcaacg atatcgccga ttcattggat gaaaccaaca ctaaggcaga cgaagccgtc 600 aaaaccgcca atgaagccaa acagacggcc gaagaaacca aacaaaacgt cgatgccaaa 660 gtaaaagctg cagaaactgc agcaggcaaa gccgaagctg ccgctggcac agctaatact 720 gcagccgaca aggccgaagc tgtcgctgca aaagttaccg acatcaaagc tgatatcgct 780 acgaacaaag ataatattgc taaaaaagca aaaattaccg acgtgtacac cagagaagag 840 tctgacagca aatttgtcag aattgatggt ctgaacgcta ctaccgaaaa attggacaca 900 cgcttggctt ctgctgaaaa atccattgcc gatcacgata ctcgcctgaa cggtttggat 960 aaaacagtgt cagacctgcg caaagaaacc cgccaaggcc ttgcagaaca agccgcgctc 1020 tccggtctgt tccaacctta caacgtgggt ggatccggag gaggaggatc agatttggca 1080 aacgattctt ttatccggca ggttctcgac cgtcagcatt tcgaacccga cgggaaatac 1140 cacctattcg gcagcagggg ggaacttgcc gagcgcagcg gccatatcgg attgggaaaa 1200 atacaaagcc atcagttggg caacctgatg attcaacagg cggccattaa aggaaatatc 1260 ggctacattg tccgcttttc cgatcacggg cacgaagtcc attccccctt cgacaaccat 1320 gcctcacatt ccgattctga tgaagccggt agtcccgttg acggatttag cctttaccgc 1380 atccattggg acggatacga acaccatccc gccgaaggct atgacgggcc acagggcggc 1440 ggctatcccg ctcccaaagg cgcgagggat atatacagct acgacataaa aggcgttgcc 1500 caaaatatcc gcctcaacct gaccgacaac cgcagcaccg gacaacggct tgccgaccgt 1560 ttccacaatg ccggtagtat gctgacgcaa ggagtaggcg acggattcaa acgcgccacc 1620 cgatacagcc ccgagctgga cagatcgggc aatgccgccg aagccttcaa cggcactgca 1680 gatatcgtta aaaacatcat cggcgcggca ggagaaattg tcggcgcagg cgatgccgtg 1740 cagggcataa gcgaaggctc aaacattgct gtcatgcacg gcttgggtct gctttccacc 1800 gaaaacaaca tggcgcgcat caacgatttg gcagatatgg cgcaactcaa agactatgcc 1860 gcagcagcca tccgcgattg ggcagtccaa aaccccaatg ccgcacaagg catagaagcc 1920 gtcagcaata tctttatggc agccatcccc atcaaaggga ttggagctgt tcggggaaaa 1980 tacggcttgg gcggcatcac ggcacatcct atcaagcggt cgcagatggg cgcgatcgca 2040 ttgccgaaag ggaaatccgc cgtcagcgac aattttgccg atgcggcata cgccaaatac 2100 ccgtcccctt accattcccg aaatatccgt tcaaacttgg agcagcgtta cggcaaagaa 2160 aacatcacct cctcaaccgt gccgccgtca aacggcaaaa atgtcaaact ggcagaccaa 2220 cgccacccga agacaggcgt accgtttgac ggtaaagggt ttccgaattt tgagaagcac 2280 gtgaaatatg atacgtaact cgag 2304 <210> 54 <211> 765 <212> PRT
<213> Artificial Sequence <220>
<223> 961cL-ORF46.1 <400> 54 Met Lys His Phe Pro Ser Lys Val Leu Thr Thr Ala Ile Leu Ala Thr Phe Cys Ser Gly Ala Leu Ala Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile Ala Ala Ala Tyr Asn Asn Gly Gln Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gln Thr Ala Glu Glu Thr Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gln Gly Leu Ala Glu Gln Ala Ala Leu Ser Gly Leu Phe Gln Pro Tyr Asn Val Gly Gly Ser Gly Gly Gly Gly Ser Asp Leu Ala Asn Asp Ser Phe Ile Arg Gln Val Leu Asp Arg Gln His Phe Glu Pro Asp Gly Lys Tyr His Leu Phe Gly Ser Arg Gly Glu Leu Ala Glu Arg Ser Gly His Ile Gly Leu Gly Lys Ile Gln Ser His Gln Leu Gly Asn Leu Met Ile Gln Gln Ala Ala Ile Lys Gly Asn Ile Gly Tyr Ile Val Arg Phe Ser Asp His Gly His Glu Val His Ser Pro Phe Asp Asn His Ala Ser His Ser Asp Ser Asp Glu Ala Gly Ser Pro Val Asp Gly Phe Ser Leu Tyr Arg Ile His Trp Asp Gly Tyr Glu His His Pro Ala Asp Gly Tyr Asp Gly Pro Gln Gly Gly Gly Tyr Pro Ala Pro Lys Gly Ala Arg Asp Ile Tyr Ser Tyr Asp Ile Lys Gly Val Ala Gln Asn Ile Arg Leu Asn Leu Thr Asp Asn Arg Ser Thr Gly Gln Arg Leu Ala Asp Arg Phe His Asn Ala Gly Ser Met Leu Thr Gln Gly Val Gly Asp Gly Phe Lys Arg Ala Thr Arg Tyr Ser Pro Glu Leu Asp Arg Ser Gly Asn Ala Ala Glu Ala Phe Asn Gly Thr Ala Asp Ile Val Lys Asn Ile Ile Gly Ala Ala Gly Glu Ile Val Gly Ala Gly Asp Ala Val Gln Gly Ile Ser Glu Gly Ser Asn Ile Ala Val Met His Gly Leu Gly Leu Leu Ser Thr Glu Asn Lys Met Ala Arg Ile Asn Asp Leu Ala Asp Met Ala Gln Leu Lys Asp Tyr Ala Ala Ala Ala Ile Arg Asp Trp Ala Val Gln Asn Pro Asn Ala Ala Gln Gly Ile Glu Ala Val Ser Asn Ile Phe Met Ala Ala Ile Pro Ile Lys Gly Ile Gly Ala Val Arg Gly Lys Tyr Gly Leu Gly Gly Ile Thr Ala His Pro Ile Lys Arg Ser Gln Met Gly Ala Ile Ala Leu Pro Lys Gly Lys Ser Ala Val Ser Asp Asn Phe Ala Asp Ala Ala Tyr Ala Lys Tyr Pro Ser Pro Tyr His Ser Arg Asn Ile Arg Ser Asn Leu Glu Gln Arg Tyr Gly Lys Glu Asn Ile Thr Ser Ser Thr Val Pro Pro Ser Asn Gly Lys Asn Val Lys Leu Ala Asp Gln Arg His Pro Lys Thr Gly Val Pro Phe Asp Gly Lys Gly Phe Pro Asn Phe Glu Lys His Val Lys Tyr Asp Thr <210> 55 <211> 1839 <212> DNA
<213> Artificial Sequence <220>
<223> 961cL-741 <400> 55 atgaaacact ttccatccaa agtactgacc acagccatcc ttgccacttt ctgtagcggc 60 gcactggcag ccacaaacga cgacgatgtt aaaaaagctg ccactgtggc cattgctgct 120 gcctacaaca atggccaaga aatcaacggt ttcaaagctg gagagaccat ctacgacatt 180 gatgaagacg gcacaattac caaaaaagac gcaactgcag ccgatgttga agccgacgac 240 tttaaaggtc tgggtctgaa aaaagtcgtg actaacctga ccaaaaccgt caatgaaaac 300 aaacaaaacg tcgatgccaa agtaaaagct gcagaatctg aaatagaaaa gttaacaacc 360 aagttagcag acactgatgc cgctttagca gatactgatg ccgctctgga tgcaaccacc 420 aacgccttga ataaattggg agaaaatata acgacatttg ctgaagagac taagacaaat 480 atcgtaaaaa ttgatgaaaa attagaagcc gtggctgat.a ccgtcgacaa gcatgccgaa 540 gcattcaacg atatcgccga ttcattggat gaaaccaaca ctaaggcaga cgaagccgtc 600 aaaaccgcca atgaagccaa acagacggcc gaagaaacca aacaaaacgt cgatgccaaa 660 gtaaaagctg cagaaactgc agcaggcaaa gccgaagctg ccgctggcac agctaatact 720 gcagccgaca aggccgaagc tgtcgctgca aaagttaccg acatcaaagc tgatatcgct 780 acgaacaaag ataatattgc taaaaaagca aacagtgccg acgtgtacac cagagaagag 840 tctgacagca aatttgtcag aattgatggt ctgaacgcta ctaccgaaaa attggacaca 900 cgcttggctt ctgctgaaaa atccattgcc gatcacgata ctcgcctgaa cggtttggat 960 aaaacagtgt cagacctgcg caaagaaacc cgccaaggcc ttgcagaaca agccgcgctc 1020 tccggtctgt tccaacctta caacgtgggt ggatccggag ggggtggtgt cgccgccgac 1080 atcggtgcgg ggcttgccga tgcactaacc gcaccgctcg accataaaga caaaggtttg 1140 cagtctttga cgctggatca gtccgtcagg aaaaacgaga aactgaagct ggcggcacaa 1200 ggtgcggaaa aaacttatgg aaacggtgac agcctcaata cgggcaaatt gaagaacgac 1260 aaggtcagcc gtttcgactt tatccgccaa atcgaagtgg acgggcagct cattaccttg 1320 gagagtggag agttccaagt atacaaacaa agccattccg ccttaaccgc ctttcagacc 1380 gagcaaatac aagattcgga gcattccggg aagatggttg cgaaacgcca gttcagaatc 1440 ggcgacatag cgggcgaaca tacatctttt gacaagcttc ccgaaggcgg cagggcgaca 1500 tatcgcggga cggcgttcgg ttcagacgat gccggcggaa aactgaccta caccatagat 1560 ttcgccgcca agcagggaaa cggcaaaatc gaacatttga aatcgccaga actcaatgtc 1620 gacctggccg ccgccgatat caagccggat ggaaaacgcc atgccgtcat cagcggttcc 1680 gtcctttaca accaagccga gaaaggcagt tactccctcg gtatctttgg cggaaaagcc 1740 caggaagttg ccggcagcgc ggaagtgaaa accgtaaacg gcatacgcca tatcggcctt 1800 gccgccaagc aactcgagca ccaccaccac caccactga 1839 <210> 56 <211> 612 <212> PRT
<213> Artificial Sequence <220>
<223> 961cL-741 <400> 56 Met Lys His Phe Pro Ser Lys Val Leu Thr Thr Ala Ile Leu Ala Thr Phe Cys Ser Gly Ala Leu Ala Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile Ala Ala Ala Tyr Asn Asn Gly Gln Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gln Thr Ala Glu Glu Thr Lys Gin Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gln Gly Leu Ala Glu Gin Ala Ala Leu Ser Gly Leu Phe Gln Pro Tyr Asn Val Gly Gly Ser Gly Gly Gly Gly Val Ala Ala Asp Ile Gly Ala Gly Leu Ala Asp Ala Leu Thr Ala Pro Leu Asp His Lys Asp Lys G].y Leu Gln Ser Leu Thr Leu Asp Gln Ser Val Arg Lys Asn Glu Lys Leu Lys Leu Ala Ala Gln Gly Ala Glu Lys Thr Tyr Gly Asn Gly Asp Ser Leu Asn Thr Gly Lys Leu Lys Asn Asp Lys Val Ser Arg Phe Asp Phe Ile Arg Gin Ile Glu Val Asp Gly Gln Leu Ile Thr Leu Glu Ser Gly Glu Phe Gln Val Tyr Lys Gln Ser His Ser Ala Leu Thr Ala Phe Gin Thr Glu Gln Ile Gln Asp Ser Glu His Ser Gly Lys Met Val Ala Lys Arg Gln Phe Arg Ile Gly Asp Ile Ala Gly Glu His Thr Ser Phe Asp Lys Leu Pro Glu Gly Gly Arg Ala Thr Tyr Arg Gly Thr Ala Phe Gly Ser Asp Asp Ala Gly Gly Lys Leu Thr Tyr Thr Ile Asp Phe Ala Ala Lys Gln Gly Asn Gly Lys Ile Glu His Leu Lys Ser Pro Glu Leu Asn Val Asp Leu Ala Ala Ala Asp Ile Lys Pro Asp Gly Lys Arg His Ala Val Ile Ser Gly Ser Val Leu Tyr Asn Gln Ala Glu Lys Gly Ser Tyr Ser Leu Gly Ile Phe Gly Gly Lys Ala Gln Glu Val Ala Gly Ser Ala Glu Val Lys Thr Val Asn Gly Ile Arg His Ile Gly Leu Ala Ala Lys Gln Leu Glu His His His His His His <210> 57 <211> 4218 <212> DNA
<213> Artificial Sequence <220>
<223> 961cL-983 <400> 57 atgaaacact ttccatccaa agtactgacc acagccatcc ttgccacttt ctgtagcggc 60 gcactggcag ccacaaacga cgacgatgtt aaaaaagctg ccactgtggc cattgctgct 120 gcctacaaca atggccaaga aatcaacggt ttcaaagctg gagagaccat ctacgacatt 180 gatgaagacg gcacaattac caaaaaagac gcaactgcag ccgatgttga agccgacgac 240 tttaaaggtc tgggtctgaa aaaagtcgtg actaacctga ccaaaaccgt caatgaaaac 300 aaacaaaacg tcgatgccaa agtaaaagct gcagaatctg aaatagaaaa gttaacaacc 360 aagttagcag acactgatgc cgctttagca gatactgatg ccgctctgga tgcaaccacc 420 aacgccttga ataaattggg agaaaatata acgacatttg ctgaagagac taagacaaat 480 atcgtaaaaa ttgatgaaaa attagaagcc gtggctgata ccgtcgacaa gcatgccgaa 540 gcattcaacg atatcgccga ttcattggat gaaaccaaca ctaaggcaga cgaagccgtc 600 aaaaccgcca atgaagccaa acagacggcc gaagaaacca aacaaaacgt cgatgccaaa 660 gtaaaagctg cagaaactgc agcaggcaaa gccgaagctg ccgctggcac agctaatact 720 gcagccgaca aggccgaagc tgtcgctgca aaagttaccg acatcaaagc tgatatcgct 780 acgaacaaag ataatattgc taaaaaagca aacagtaccg acgtgtacac cagagaagag 840 tctgacagca aatttgtcag aattgatggt ctgaacgcta ctaccgaaaa attggacaca 900 cgcttggctt ctgctgaaaa atccattgcc gatcacgata ctcgcctgaa cggtttggat 960 aaaacagtgt cagacctgcg caaagaaacc cgccaaggcc ttgcagaaca agccgcgctc 1020 tccggtctgt tccaacctta caacgtgggt ggatccggcg gaggcggcac ttctgcgccc 1080 gacttcaatg caggcggtac cggtatcggc agcaaaagca gagcaacaac agcgaaatca 1140 gcagcagtat cttacgccgg tatcaagaac gaaatgtgca aagacagaag catgctctgt 1200 gccggtcggg atgacgttgc ggttacagac agggatgcca aaatcaatgc cccccccccg 1260 aatctgcata ccggagactt tccaaaccca aatgacgcat acaagaattt gatcaacctc 1320 aaacctgcaa ttgaagcagg ctatacagga cgcggggtag aggtaggtat cgtcgacaca 1380 ggcaaatccg tcggcagcat atcctttccc gaactgtatg gcagaaaaga acacggctat 1440 aacgaaaatt acaaaaacta tacggcgtat atgcggaagg aagcgcctga agacggaggc 1500 ggtaaagaca ttgaagcttc tttcgacgat gaggccgtta tagagactga agcaaagccg 1560 acgtatatac gccacgtaaa agaaatcgga cacatcgat:t tggtctccca tattattggc 1620 gggcgttccg tggacggcag acctgcaggc ggtattgcgc ccgatgccac gctacacata 1680 atgaatacga atgatgaaac caagaacgaa atgatggttg cagccatccg caatgcatgg 1740 gtcaagctgg gcgaacgtgg cgtgcgcatc gtcaataaca gttttggaac aacatcgagg 1800 gcaggcactg ccgacctttt ccaaatagcc aattcggagg agcagtaccg ccaagcgttg 1860 ctcgactatt ccggcggtga taaaacagac gagggtatcc gcctgatgca acagagcgat 1920 tacggcaacc tgtcctacca catccgtaat aaaaacatgc ttttcatctt ttcgacaggc 1980 aatgacgcac aagctcagcc caacacatat gccctattgc cattttatga aaaagacgct 2040 caaaaaggca ttatcacagt cgcaggcgta gaccgcagtg gagaaaagtt caaacgggaa 2100 atgtatggag aaccgggtac agaaccgctt gagtatggct ccaaccattg cggaattact 2160 gccatgtggt gcctgtcggc accctatgaa gcaagcgtcc gtttcacccg tacaaacccg 2220 attcaaattg ccggaacatc cttttccgca cccatcgtaa ccggcacggc ggctctgctg 2280 ctgcagaaat acccgtggat gagcaacgac aatctgcgta ccacgttgct gacgacggct 2340 caggacatcg gtgcagccgg cgtggacagc aagttcggct ggggactgct ggatgcgggt 2400 aaggccatga acggacccgc gtcctttccg ttcggcgact ttaccgccga tacgaaaggt 2460 acatccgata ttgcctactc cttccgtaac gacatttcag gcacgggcgg cctgatcaaa 2520 aaaggcggca gccaactgca actgcacggc aacaacacct atagcggcaa aaccattatc 2580 gaaggcggtt cgctggtgtt gtacggcaac aacaaatcgg atatgcgcgt cgaaaccaaa 2640 ggtgcgctga tttataacgg ggcggcatcc ggcggcagcc tgaacagcga cggcattgtc 2700 tatctggcag ataccgacca atccggcgca aacgaaaccg tacacatcaa aggcagtctg 2760 cagctggacg gcaaacgtac gctgtacaca cgtttgggca aactgctgaa attggacggt 2820 acggcgatta tcggcggcaa gctgtacatg tcggcacgcg gcaagggggc aggctatctc 2880 aacagtaccg gacgacgtgt tcccttcctg agtgccgcca aaatcgggca ggattattct 2940 ttcttcacaa acatcgaaac cgacggcggc ctgctggctt ccctcgacag cgtcgaaaaa 3000 acagcgggca gtgaaggcga cacgctgtcc tattatgtcc gtcgcggcaa tgcggcacgg 3060 actgcttcgg cagcggcaca ttccgcgccc gccggtctga aacacgccgt agaacagggc 3120 ggcagcaatc tggaaaacct gatggtcgaa ctggatgcct ccgaatcatc cgcaacaccc 3180 gagacggttg aaactgcggc agccgaccgc acagatatgc cgggcatgcg cccctacggc 3240 gcaactttcc gcgcagcggc agccgtacag catgcgaatg ccgccgacgg tgtacgcatc 3300 ttcaacagtc tcgccgctac cgtctatgcc gacagtaccg ccgcccatgc cgatatgcag 3360 ggacgccgcc tgaaagccgt atcggacggg ttggaccaca acggcacggg tctgcgcgtc 3420 atcgcgcaaa cccaacagga cggtggaacg tgggaacagg gcggtgttga aggcaaaatg 3480 cgcggcagta cccaaaccgt cggcattgcc gcgaaaaccg gcgaaaatac gacagcagcc 3540 gccacactgg gcatgggacg cagcacatgg agcgaaaaca gtgcaaatgc aaaaaccgac 3600 agcattagtc tgtttgcagg catacggcac gatgcgggcg atatcggcta tctcaaaggc 3660 ctgttctcct acggacgcta caaaaacagc atcagccgca gcaccggtgc ggacgaacat 3720 gcggaaggca gcgtcaacgg cacgctgatg cagctgggcg cactgggcgg tgtcaacgtt 3780 ccgtttgccg caacgggaga tttgacggtc gaaggcggtc tgcgctacga cctgctcaaa 3840 caggatgcat tcgccgaaaa aggcagtgct ttgggctgga gcggcaacag cctcactgaa 3900 ggcacgctgg tcggactcgc gggtctgaag ctgtcgcaac ccttgagcga taaagccgtc 3960 ctgtttgcaa cggcgggcgt ggaacgcgac ctgaacggac gcgactacac ggtaacgggc 4020 ggctttaccg gcgcgactgc agcaaccggc aagacggggg cacgcaatat gccgcacacc 4080 cgtctggttg ccggcctggg cgcggatgtc gaattcggca acggctggaa cggcttggca 4140 cgttacagct acgccggttc caaacagtac ggcaaccaca gcggacgagt cggcgtaggc 4200 taccggttct gactcgag 4218 <210> 58 <211> 1403 <212> PRT
<213> Artificial Sequence <220>
<223> 961cL-983 <400> 58 Met Lys His Phe Pro Ser Lys Val Leu Thr Thr Ala Ile Leu Ala Thr Phe Cys Ser Gly Ala Leu Ala Ala Thr Asn Asp Asp Asp Val Lys Lys Ala Ala Thr Val Ala Ile Ala Ala Ala Tyr Asn Asn Gly Gin Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Asp Glu Asp Gly Thr Ile Thr Lys Lys Asp Ala Thr Ala Ala Asp Val Glu Ala Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr Lys Thr Val Asn Glu Asn Lys Gin Asn Val Asp Ala Lys Val Lys Ala Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Ala Thr Thr Asn Ala Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala Lys Gin Thr Ala Glu Glu Thr Lys Gln Asn Val Asp Ala Lys Val Lys Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp Ile Lys Ala Asp Ile Ala Thr Asn Lys Asp Asn Ile Ala Lys Lys Ala Asn Ser Ala Asp Val Tyr Thr Arg Glu Glu Ser Asp Ser Lys Phe Val Arg Ile Asp Gly Leu Asn Ala Thr Thr Glu Lys Leu Asp Thr Arg Leu Ala Ser Ala Glu Lys Ser Ile Ala Asp His Asp Thr Arg Leu Asn Gly Leu Asp Lys Thr Val Ser Asp Leu Arg Lys Glu Thr Arg Gln Gly Leu Ala Glu Gln Ala Ala Leu Ser Gly Leu Phe Gln Pro Tyr Asn Val Gly Gly Ser Gly Gly Gly Gly Thr Ser Ala Pro Asp Phe Asn Ala Gly Gly Thr Gly Ile Gly Ser Asn Ser Arg Ala Thr Thr Ala Lys Ser Ala Ala Val Ser Tyr Ala Gly Ile Lys Asn Glu Met Cys Lys Asp Arg Ser Met Leu Cys Ala Gly Arg Asp Asp Val Ala Val Thr Asp Arg Asp Ala Lys Ile Asn Ala Pro Pro Pro Asn Leu His Thr Gly Asp Phe Pro Asn Pro Asn Asp Ala Tyr Lys Asn Leu Ile Asn Leu Lys Pro Ala Ile Glu Ala Gly Tyr Thr Gly Arg Gly Val Glu Val Gly Ile Val Asp Thr Gly Glu Ser Val Gly Ser Ile Ser Phe Pro Glu Leu Tyr Gly Arg Lys Glu His Gly Tyr Asn Glu Asn Tyr Lys Asn Tyr Thr Ala Tyr Met Arg Lys Glu Ala Pro Glu Asp Gly Gly Gly Lys Asp Ile Glu Ala Ser Phe Asp Asp Glu Ala Val Ile Glu Thr Glu Ala Lys Pro Thr Asp Ile Arg His Val Lys Glu Ile Gly His Ile Asp Leu Val Ser His Ile Ile Gly Gly Arg Ser Val Asp Gly Arg Pro Ala Gly Gly Ile Ala Pro Asp Ala Thr Leu His Ile Met Asn Thr Asn Asp Glu Thr Lys Asn Glu Met Met Val Ala Ala Ile Arg Asn Ala Trp Val Lys Leu Gly Glu Arg Gly Val Arg Ile Val Asn Asn Ser Phe Gly Thr Thr Ser Arg Ala Gly Thr Ala Asp Leu Phe Gln Ile Ala Asn Ser Glu Glu Gln Tyr Arg Gln Ala Leu Leu Asp Tyr Ser Gly Gly Asp Lys Thr Asp Glu Gly Ile Arg Leu Met Gln Gln Ser Asp Tyr Gly Asn Leu Ser Tyr His Ile Arg Asn Lys Asn Met Leu Phe Ile Phe Ser Thr Gly Asn Asp Ala Gln Ala Gin Pro Asn Thr Tyr Ala Leu Leu Pro Phe Tyr Glu Lys Asp Ala Gln Lys Gly Ile Ile Thr Val Ala Gly Val Asp Arg Ser Gly Glu Lys Phe Lys Arg Glu Met Tyr Gly Glu Pro Gly Thr Glu Pro Leu Glu Tyr Gly.Ser Asn His Cys Gly Ile Thr 705 710 71.5 720 Ala Met Trp Cys Leu Ser Ala Pro Tyr Giu Ala Ser Val Arg Phe Thr Arg Thr Asn Pro Ile Gln Ile Ala Gly Thr Ser Phe Ser Ala Pro Ile Val Thr Gly Thr Ala Ala Leu Leu Leu Gln Lys Tyr Pro Trp Met Ser Asn Asp Asn Leu Arg Thr Thr Leu Leu Thr Thr Ala Gln Asp Ile Gly Ala Val Gly Val Asp Ser Lys Phe Gly Trp Gly Leu Leu Asp Ala Gly Lys Ala Met Asn Gly Pro Ala Ser Phe Pro Phe Gly Asp Phe Thr Ala Asp Thr Lys Gly Thr Ser Asp Ile Ala Tyr Ser Phe Arg Asn Asp Ile Ser Gly Thr Gly Gly Leu Ile Lys Lys Gly Gly Ser Gln Leu Gln Leu His Gly Asn Asn Thr Tyr Thr Gly Lys Thr Ile Ile Glu Gly Gly Ser Leu Val Leu Tyr Gly Asn Asn Lys Ser Asp Met Arg Val Glu Thr Lys Gly Ala Leu Ile Tyr Asn Gly Ala Ala Ser Gly Gly Ser Leu Asn Ser Asp Gly Ile Val Tyr Leu Ala Asp Thr Asp Gln Ser Gly Ala Asn Glu Thr Val His Ile Lys Gly Ser Leu Gln Leu Asp Gly Lys Gly Thr Leu Tyr Thr Arg Leu Gly Lys Leu Leu Lys Val Asp Gly Thr Ala Ile Ile Gly Gly Lys Leu Tyr Met Ser Ala Arg Gly Lys Gly Ala Gly Tyr Leu Asn Ser Thr Gly Arg Arg Val Pro Phe Leu Ser Ala Ala Lys Ile Gly Gln Asp Tyr Ser Phe Phe Thr Asn Ile Glu Thr Asp Gly Gly Leu Leu Ala Ser Leu Asp Ser Val Glu Lys Thr Ala Gly Ser Glu Gly Asp Thr Leu Ser Tyr Tyr Val Arg Arg Gly Asn Ala Ala Arg Thr Ala Ser Ala Ala Ala His Ser Ala Pro Ala Gly Leu Lys His Ala Val Glu Gln Gly Gly Ser Asn Leu Glu Asn Leu Met Val Glu Leu Asp Ala Ser Glu Ser Ser Ala Thr Pro Glu Thr Val Glu Thr Ala Ala Ala Asp Arg Thr Asp Met Pro Gly Ile Arg Pro Tyr Gly Ala Thr Phe Arg Ala Ala Ala Ala Val Gln His Ala Asn Ala Ala Asp Gly Val Arg Ile Phe Asn Ser Leu Ala Ala Thr Val Tyr Ala Asp Ser Thr Ala Ala His Ala Asp Met Gln Gly Arg Arg Leu Lys Ala Val Ser Asp Gly Leu Asp His Asn Gly Thr 1125 1.130 1135 Gly Leu Arg Val Ile Ala Gln Thr Gln Gln Asp Gly Gly Thr Trp Glu Gln Gly Gly Val Glu Gly Lys Met Arg Gly Ser Thr Gln Thr Val Gly Ile Ala Ala Lys Thr Gly Glu Asn Thr Thr Ala Ala Ala Thr Leu Gly Met Gly Arg Ser Thr Trp Ser Glu Asn Ser Ala Asn Ala Lys Thr Asp Ser Ile Ser Leu Phe Ala Gly Ile Arg His Asp Ala Gly Asp Ile Gly Tyr Leu Lys Gly Leu Phe Ser Tyr Gly Arg Tyr Lys Asn Ser Ile Ser Arg Ser Thr Gly Ala Asp Glu His Ala Glu Gly Ser Val Asn Gly Thr Leu Met Gln Leu Gly Ala Leu Gly Gly Val Asn Val Pro Phe Ala Ala Thr Gly Asp Leu Thr Val Glu Gly Gly Leu Arg Tyr Asp Leu Leu Lys Gln Asp Ala Phe Ala Glu Lys Gly Ser Ala Leu Gly Trp Ser Gly Asn Ser Leu Thr Glu Gly Thr Leu Val Gly Leu Ala Gly Leu Lys Leu Ser Gln Pro Leu Ser Asp Lys Ala Val Leu Phe Ala Thr Ala Gly Val Glu Arg Asp Leu Asn Gly Arg Asp Tyr Thr Val Thr Gly Gly Phe Thr Gly Ala Thr Ala Ala Thr Gly Lys Thr Gly Ala Arg Asn Met Pro His Thr Arg Leu Val Ala Gly Leu Gly Ala Asp Val Glu Phe Gly Asn Gly Trp Asn Gly Leu Ala Arg Tyr Ser Tyr Ala Gly Ser Lys Gln Tyr Gly Asn His Ser Gly Arg Val Gly Val Gly Tyr Arg Phe <210> 59 <211> 25 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 59 cgcggatccg gagggggtgg tgtcg 25 <210> 60 <211> 27 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 60 cccgctcgag ttgcttggcg gcaaggc 27 <210> 61 <211> 25 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 61 cgcggatccg gcggaggcgg cacti 25 <210> 62 <211> 26 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 62 cccgctcgag gaaccggtag cctacg 26 <210> 63 <211> 41 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 63 cgcggatccg gtggtggtgg ttcagatttg gcaaacgatt c 41 <210> 64 <211> 29 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 64 cccgctcgag cgtatcatat ttcacgtgc 29 <210> 65 <211> 25 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 65 cgcggatccg gagggggtgg tgtcg 25 <210> 66 <211> 28 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 66 cccgctcgag ttattgcttg gcggcaag 28 <210> 67 <211> 25 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 67 cgcggatccg gcggaggcgg cactt 25 <210> 68 <211> 28 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 68 cccgctcgag tcagaaccgg tagcctac 28 <210> 69 <211> 41 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 69 cgcggatccg gtggtggtgg ttcagatttg gcaaacgatt c 41 <210> 70 <211> 32 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 70 cccgctcgag ttacgtatca tatttcacgt gc 32 <210> 71 <211> 42 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 71 cgcggatccg gtggtggtgg tcaaagcaag agcatccaaa cc 42 <210> 72 <211> 30 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 72 cccaagcttt tcgggcggta ttcgggcttc 30 <210> 73 <211> 39 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 73 cgcggatccg gtggtggtgg tgccacctac aaagtggac 39 <210> 74 <211> 28 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 74 gcccaagctt ttgtttggct gcctcgat 28 <210> 75 <211> 34 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 75 cgcggatccg gtggtggtgg tacaagcgac gacg 34 <210> 76 <211> 28 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 76 gcccaagctt ccactcgtaa ttgacgcc 28 <210> 77 <211> 41 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 77 cgcggatccg gtggtggtgg ttcagatttg gcaaacgatt c 41 <210> 78 <211> 28 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 78 cccaagcttc gtatcatatt tcacgtgc 28 <210> 79 <211> 44 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 79 cccaagcttg gtggtggtgg tggttcagat ttgtcaaacg attc 44 <210> 80 <211> 29 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 80 cccgctcgag cgtatcatat ttcacgtgc 29 <210> 81 <211> 45 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 81 cccaagcttg gtggtggtgg tggtcaaagc aagagcatcc aaacc 45 <210> 82 <211> 28 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 82 cccgctcgag cgggcggtat tcgggctt 28 <210> 83 <211> 32 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 83 cgcggatccg ctagccccga tgttaaatcg gc 32 <210> 84 <211> 29 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 84 cggggatcca tcctgctctt ttttgccgg 29 <210> 85 <211> 36 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 85 cgcggatccg ctagcggaca cacttatttc ggcatc 36 <210> 86 <211> 30 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 86 cgcggatccc cagcggtagc ctaatttgat 30 <210> 87 <211> 41 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 87 cgcggatccg gtggtggtgg ttcagatttg gcaaacgatt c 41 <210> 88 <211> 28 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 88 cccaagcttc gtatcatatt tcacgtgc 28 <210> 89 <211> 36 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 89 gcggcgtcga cggtggcgga ggcactggat cctcag 36 <210> 90 <211> 35 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 90 ggaggcactg gatcctcaga tttggcaaac gattc 35 <210> 91 <211> 29 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 91 cccgctcgag cgtatcatat ttcacgtgc 29 <210> 92 <211> 25 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 92 cggggatccg ggggcggcgg tggcg 25 <210> 93 <211> 30 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 93 cccaagctta tcctgctctt ttttgccggc 30 <210> 94 <211> 42 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 94 cgcggatccg gtggtggtgg tcaaagcaag agcatccaaa cc 42 <210> 95 <211> 28 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 95 cccaagcttc gggcggtatt cgggcttc 28 <210> 96 <211> 26 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 96 ccccaagctt gggggcggcg gtggcg 26 <210> 97 <211> 31 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 97 cccgctcgag atcctgctct tttttgccgg c 31 <210> 98 <211> 45 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 98 cccaagcttg gtggtggtgg tggtcaaagc aagagcatcc aaacc 45 <210> 99 <211> 28 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 99 cccgctcgag cgggcggtat tcgggctt 28 <210> 100 <211> 35 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 100 ggaggcactg gatccgcagc cacaaacgac gacga 35 <210> 101 <211> 36 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 101 gcggcctcga gggtggcgga ggcactggat ccgcag 36 <210> 102 <211> 28 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 102 cccgctcgag acccagcttg taaggttg 28 <210> 103 <211> 35 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 103 ggaggcactg gatccgcagc cacaaacgac gacga 35 <210> 104 <211> 36 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 104 gcggcctcga gggtggcgga ggcactggat ccgcag 36 <210> 105 <211> 28 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 105 cccgctcgag ccactcgtaa ttgacgcc 28 <210> 106 <211> 38 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 106 gcggcctcga gggatccggc ggaggcggca cttctgcg 38 <210> 107 <211> 26 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 107 cccgctcgag gaaccggtag cctacg 26 <210> 108 <211> 35 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 108 ggaggcactg gatcctcaga tttggcaaac gattc 35 <210> 109 <211> 37 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 109 gcggcgtcga cggtggcgga ggcactggat cctcaga 37 <210> 110 <211> 29 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 110 cccgctcgag cgtatcatat ttcacgtgc 29 <210> 111 <211> 35 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 111 gcggcctcga gggatccgga gggggtggtg tcgcc 35 <210> 112 <211> 25 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 112 cccgctcgag ttgcttggcg gcaag 25 <210> 113 <211> 35 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 113 ggaggcactg gatccgcagc cacaaacgac gacga 35 <210> 114 <211> 36 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 114 gcggcctcga gggtggcgga ggcactggat ccgcag 36 <210> 115 <211> 28 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 115 cccgctcgag acccagcttg taaggttg 28 <210> 116 <211> 35 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 116 ggaggcactg gatccgcagc cacaaacgac gacga 35 <210> 117 <211> 36 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 117 gcggcctcga gggtggcgga ggcactggat ccgcag 36 <210> 118 <211> 28 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 118 cccgctcgag ccactcgtaa ttgacgcc 28 <210> 119 <211> 35 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 119 ggaggcactg gatcctcaga tttggcaaac gattc 35 <210> 120 <211> 37 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 120 gcggcgtcga cggtggcgga ggcactggat cctcaga 37 <210> 121 <211> 29 <212> DNA
<213> Artificial Sequence <220>
<223> Oligonucleotide <400> 121 cccgctcgag cgtatcatat ttcacgtgc 29

Claims (5)

CLAIMS:
1. A hybrid protein of formula NH2-A-B-COOH, wherein A comprises the Neisserial protein .DELTA.G287 and B comprises the Neisserial protein 953, and wherein the amino acid sequence of the hybrid protein is as disclosed in SEQ ID NO: 6 or is a sequence having greater than 70% sequence identity thereto.
2. The protein of claim 1, wherein .DELTA.G287 is from N. meningitidis strain 2996 or N. meningitidis strain 394/98.
3. The protein of claim 1, wherein 953 is from N. meningitidis strain 2996 or N. meningitidis strain 394/98.
4. The protein of claim 1, wherein A and B are from the same strain of N. meningitidis.
5. The protein of claim 1, comprising the amino acid sequence recited in SEQ
ID
NO: 6.
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Families Citing this family (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2298900A1 (en) 1996-09-17 2011-03-23 Novartis Vaccines and Diagnostics, Inc. Compositions and methods for treating intracellular diseases
DE69941567D1 (en) * 1998-01-14 2009-12-03 Novartis Vaccines & Diagnostic ANTIGENE FROM NEISSERIA MENINGITIDIS
CN101293920B (en) 1998-05-01 2012-07-18 诺华疫苗和诊断公司 Neisseria meningitidis antigens and compositions
US20070026021A1 (en) * 1998-05-01 2007-02-01 Chiron S.R.I. Neisseria meningitidis antigens and compositions
ES2228454T3 (en) * 1999-02-26 2005-04-16 Chiron S.R.L. IMPROVEMENT OF THE BACTERIAL ACTIVITY OF NEISSERIA ANTIGENS WITH OLIGONUCLEOTIDES CONTAINING MOTIVES CG.
NZ530640A (en) 1999-04-30 2006-06-30 Chiron S Conserved neisserial antigens
GB9911683D0 (en) * 1999-05-19 1999-07-21 Chiron Spa Antigenic peptides
CA2929348A1 (en) * 1999-05-19 2000-11-30 Novartis Vaccines And Diagnostics S.R.L. Combination neisserial compositions
AU784203B2 (en) 1999-10-29 2006-02-23 Glaxosmithkline Biologicals S.A. Neisserial antigenic peptides
GB9928196D0 (en) 1999-11-29 2000-01-26 Chiron Spa Combinations of B, C and other antigens
RU2279889C2 (en) * 2000-01-17 2006-07-20 Чирон С.Р.Л. OUTER MEMBRANE VESICLE VACCINE (OMV) CONTAINING PROTEINS OF SEROGROUP B N.Meningitis OUTER MEMBRANE
ES2391153T3 (en) * 2000-02-28 2012-11-22 Novartis Vaccines And Diagnostics S.R.L. Heterologous expression of Neisseria proteins
US7534444B2 (en) * 2001-04-17 2009-05-19 Novattis Vaccines And Diagnostics, Inc. Molecular mimetics of meningococcal B epitopes which elicit functionally active antibodies
GB0115176D0 (en) 2001-06-20 2001-08-15 Chiron Spa Capular polysaccharide solubilisation and combination vaccines
US6928463B1 (en) * 2001-07-06 2005-08-09 Nortel Networks Limited Broadband content delivery via personal content tunnel
GB0118249D0 (en) 2001-07-26 2001-09-19 Chiron Spa Histidine vaccines
GB0121591D0 (en) * 2001-09-06 2001-10-24 Chiron Spa Hybrid and tandem expression of neisserial proteins
DK2248822T3 (en) * 2001-07-27 2017-02-13 Glaxosmithkline Biologicals Sa MENINGOCOKKER ADHESIONS
AU2014201962B2 (en) * 2001-09-06 2016-06-09 Novartis Vaccines And Diagnostics S.R.L. Hybrid and tandem expression of Neisserial proteins
AR045702A1 (en) 2001-10-03 2005-11-09 Chiron Corp COMPOSITIONS OF ASSISTANTS.
US7838015B2 (en) 2001-10-03 2010-11-23 Novartis Vaccines And Diagnostics, Inc. Adjuvanted meningococcus compositions
CN100354297C (en) * 2001-10-03 2007-12-12 希龙公司 Adjuvanted meningococcus compositions
MX339524B (en) * 2001-10-11 2016-05-30 Wyeth Corp Novel immunogenic compositions for the prevention and treatment of meningococcal disease.
EP1961427A3 (en) 2002-08-02 2009-11-04 GlaxoSmithKline Biologicals S.A. Neisserial vaccine compositions comprising a combination of antigens
US7785608B2 (en) 2002-08-30 2010-08-31 Wyeth Holdings Corporation Immunogenic compositions for the prevention and treatment of meningococcal disease
DE60335477D1 (en) 2002-10-11 2011-02-03 Novartis Vaccines & Diagnostic POLYPEPTIDE FOR WIDE PROTECTION AGAINST HYPERVIRULENT MENINGOKOKKIN LINES
US7927858B2 (en) 2002-11-01 2011-04-19 Glaxosmithkline Biologicals, S.A. Drying process
AU2003288660A1 (en) 2002-11-15 2004-06-15 Chiron Srl Unexpected surface proteins in neisseria meningitidis
GB0227346D0 (en) 2002-11-22 2002-12-31 Chiron Spa 741
EP2172213B1 (en) * 2003-01-30 2013-04-03 Novartis AG Injectable vaccines against multiple meningococcal serogroups
US7851433B2 (en) 2003-08-13 2010-12-14 Novartis Vaccines And Diagnostics, Inc. Method of purifying TFPI and TFPI analogs
PL1961426T3 (en) 2003-10-02 2012-03-30 Gsk Vaccines S R L Combined meningitis vaccines
GB0323103D0 (en) 2003-10-02 2003-11-05 Chiron Srl De-acetylated saccharides
GB0408977D0 (en) * 2004-04-22 2004-05-26 Chiron Srl Immunising against meningococcal serogroup Y using proteins
GB0409748D0 (en) 2004-04-30 2004-06-09 Chiron Srl Lactoferrin cleavage
GB0419408D0 (en) * 2004-09-01 2004-10-06 Chiron Srl 741 chimeric polypeptides
GB0424092D0 (en) 2004-10-29 2004-12-01 Chiron Srl Immunogenic bacterial vesicles with outer membrane proteins
SI2351772T1 (en) 2005-02-18 2016-11-30 Glaxosmithkline Biologicals Sa Proteins and nucleic acids from meningitis/sepsis-associated Escherichia coli
ES2385045T3 (en) 2005-02-18 2012-07-17 Novartis Vaccines And Diagnostics, Inc. Urotogenic Escherichia coli immunogens
CN100475965C (en) * 2005-07-22 2009-04-08 上海高科联合生物技术研发有限公司 Method for E, coli to express lysostaphin in high efficiency via external secretion
GB0524066D0 (en) 2005-11-25 2006-01-04 Chiron Srl 741 ii
EP1962902A2 (en) * 2005-12-06 2008-09-03 Universita Degli Studi di Padova Methods and compositions relating to adhesins as adjuvants
AU2007285484B2 (en) 2006-08-16 2013-05-02 Novartis Ag Immunogens from uropathogenic Escherichia coli
AR064642A1 (en) 2006-12-22 2009-04-15 Wyeth Corp POLINUCLEOTIDE VECTOR THAT INCLUDES IT RECOMBINATING CELL THAT UNDERSTANDS THE VECTOR POLYPEPTIDE, ANTIBODY, COMPOSITION THAT UNDERSTANDS THE POLINUCLEOTIDE, VECTOR, RECOMBINATING CELL POLYPEPTIDE OR ANTIBODY, USE OF THE COMPOSITION AND A COMPOSITION AND A METHOD
GB0700562D0 (en) 2007-01-11 2007-02-21 Novartis Vaccines & Diagnostic Modified Saccharides
GB0713880D0 (en) 2007-07-17 2007-08-29 Novartis Ag Conjugate purification
EP2200642B1 (en) 2007-10-19 2012-04-18 Novartis AG Meningococcal vaccine formulations
AU2009215364B2 (en) * 2008-02-21 2014-09-18 Glaxosmithkline Biologicals S.A. Meningococcal fHBP polypeptides
MY150481A (en) 2008-03-03 2014-01-30 Irm Llc Compounds and compositions as tlr activity modulators
CA2726512A1 (en) * 2008-06-09 2009-12-17 Novartis Ag Antibodies against neisserial factor h binding protein
JP5487463B2 (en) 2008-08-08 2014-05-07 独立行政法人産業技術総合研究所 Non-diffusing plant virus vector
CA2756522C (en) 2009-03-24 2018-06-26 Novartis Ag Adjuvanting meningococcal factor h binding protein
US9517263B2 (en) 2009-06-10 2016-12-13 Glaxosmithkline Biologicals Sa Benzonaphthyridine-containing vaccines
WO2011008400A2 (en) 2009-06-16 2011-01-20 Novartis Ag High-throughput complement-mediated antibody-dependent and opsonic bactericidal assays
AU2010288240B2 (en) 2009-08-27 2014-03-27 Novartis Ag Hybrid polypeptides including meningococcal fHBP sequences
TWI445708B (en) 2009-09-02 2014-07-21 Irm Llc Compounds and compositions as tlr activity modulators
ES2443952T3 (en) 2009-09-02 2014-02-21 Novartis Ag Immunogenic compositions that include modulators of TLR activity
CN102724988B (en) 2009-09-30 2014-09-10 诺华股份有限公司 Expression of meningococcal fHBP polypeptides
JP5960055B2 (en) 2009-10-27 2016-08-02 ノバルティス アーゲー Modified meningococcal fHBP polypeptide
WO2011057148A1 (en) 2009-11-05 2011-05-12 Irm Llc Compounds and compositions as tlr-7 activity modulators
SG181712A1 (en) 2009-12-15 2012-07-30 Novartis Ag Homogeneous suspension of immunopotentiating compounds and uses thereof
CA2792938C (en) 2010-03-23 2018-07-31 Irm Llc Compounds (cystein based lipopeptides) and compositions as tlr2 agonists used for treating infections, inflammations, respiratory diseases etc.
AU2011268507B2 (en) 2010-06-25 2014-08-14 Novartis Ag Combinations of meningococcal factor H binding proteins
EP3246044B2 (en) 2010-08-23 2024-04-10 Wyeth LLC Stable formulations of neisseria meningitidis rlp2086 antigens
ES2864635T3 (en) 2010-09-10 2021-10-14 Wyeth Llc Non-lipidated variants of Neisseria meningitidis ORF2086 antigens
AU2011300418B2 (en) 2010-09-10 2016-05-12 Glaxosmithkline Biologicals Sa Meningococcus overexpressing NadA and/or NHBA and outer membrane vesicles derived therefrom
GB201102090D0 (en) * 2011-02-08 2011-03-23 Univ Sheffield Antigenic polypeptide
JP2014529407A (en) 2011-08-31 2014-11-13 チルドレンズホスピタル アンド リサーチ センター オークランド Engineered sequences and methods of use for promoting antigen expression in Neisseria
MY167723A (en) 2012-03-09 2018-09-21 Pfizer Neisseria meningitidis compositions and methods thereof
SA115360586B1 (en) 2012-03-09 2017-04-12 فايزر انك Neisseria meningitidis compositions and methods thereof
US10376573B2 (en) 2012-06-14 2019-08-13 Glaxosmithkline Biologicals Sa Vaccines for serogroup X meningococcus
JP2015524418A (en) 2012-07-27 2015-08-24 アンスティテュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル CD147 as a receptor for pili-mediated adhesion of Neisseria meningitidis to vascular endothelial cells
WO2014084432A1 (en) * 2012-11-30 2014-06-05 경상대학교 산학협력단 Helicobacter pylori expression vector
ES2685894T3 (en) 2013-03-08 2018-10-15 Pfizer Inc. Immunogenic fusion polypeptides
WO2015033251A2 (en) 2013-09-08 2015-03-12 Pfizer Inc. Neisseria meningitidis compositions and methods thereof
CA2940447C (en) 2014-02-28 2023-07-11 Glaxosmithkline Biologicals Sa Modified meningococcal fhbp polypeptides
KR20190049940A (en) 2015-02-19 2019-05-09 화이자 인코포레이티드 Neisseria meningitidis compositions and methods thereof
GB201522153D0 (en) 2015-12-15 2016-01-27 Univ Southampton Meningococcal infection and modified neisseria lactamica
US20180064801A1 (en) 2016-09-02 2018-03-08 Glaxosmithkline Biologicals Sa Vaccines for neisseria gonorrhoeae
EP3312192B1 (en) * 2016-10-24 2023-02-22 BiOMVis Srl Immunogenic compositions containing bacterial outer membrane vesicles
AU2018215585B2 (en) 2017-01-31 2022-03-17 Pfizer Inc. Neisseria meningitidis compositions and methods thereof
CN110516550B (en) * 2019-07-26 2022-07-05 电子科技大学 FPGA-based lane line real-time detection method
CA3211240A1 (en) 2021-02-19 2022-08-25 Sanofi Pasteur Inc. Meningococcal b recombinant vaccine
GB202208093D0 (en) 2022-06-01 2022-07-13 Glaxosmithkline Biologicals Sa Immunogenic composition
GB202208089D0 (en) 2022-06-01 2022-07-13 Glaxosmithkline Biologicals Sa Immunogenic composition
TW202423477A (en) 2022-08-03 2024-06-16 美商賽諾菲巴斯德公司 Adjuvanted immunogenic composition against neisseria meningitidis b

Family Cites Families (131)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4239749A (en) 1979-09-27 1980-12-16 United States Of America Neisseria gonorrhoeae vaccine
WO1995013796A1 (en) 1993-11-16 1995-05-26 Depotech Corporation Vesicles with controlled release of actives
US5288641A (en) 1984-06-04 1994-02-22 Arch Development Corporation Herpes Simplex virus as a vector
CA1282721C (en) 1984-06-04 1991-04-09 Bernard Roizman Herpes simplex virus as a vector
EP0273116A3 (en) 1986-10-09 1990-05-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Gonococcal and meningococcal polypeptides, vaccines and diagnostics
GB8702816D0 (en) 1987-02-07 1987-03-11 Al Sumidaie A M K Obtaining retrovirus-containing fraction
US5270176A (en) * 1987-11-20 1993-12-14 Hoechst Aktiengesellschaft Method for the selective cleavage of fusion proteins with lysostaphin
CA1340506C (en) * 1987-11-24 1999-04-20 Nicholas H. Carbonetti Production of gonorrheal pi proteins and vaccines
US5591624A (en) 1988-03-21 1997-01-07 Chiron Viagene, Inc. Retroviral packaging cell lines
CN1038306A (en) 1988-03-21 1989-12-27 维吉恩公司 Recombinant retroviruses
US5422120A (en) 1988-05-30 1995-06-06 Depotech Corporation Heterovesicular liposomes
AP129A (en) 1988-06-03 1991-04-17 Smithkline Biologicals S A Expression of retrovirus gag protein eukaryotic cells
NL8803111A (en) 1988-12-19 1990-07-16 Nederlanden Staat Meningococcus class 1 outer-membrane protein vaccine - useful to immunise against meningococcal disease
AU640118B2 (en) 1988-12-19 1993-08-19 De Staat Der Nederlanden Vertegenwoordigd Door De Minister Van Welzijn, Volksgezonheid En Cultuur Meningococcal class 1 outer-membrane protein vaccine
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
DE69034078T2 (en) 1989-03-21 2004-04-01 Vical, Inc., San Diego Expression of exogenous polynucleotide sequences in vertebrates
GB8919607D0 (en) 1989-08-30 1989-10-11 Wellcome Found Novel entities for cancer therapy
CU22302A1 (en) 1990-09-07 1995-01-31 Cigb Codifying nucleotidic sequence for a protein of the external membrane of neisseria meningitidis and the use of that protein in preparing vaccines.
EP0467714A1 (en) 1990-07-19 1992-01-22 Merck & Co. Inc. The class II protein of the outer membrane of neisseria meningitidis
JPH06500923A (en) 1990-09-21 1994-01-27 カイロン　コーポレイション packaging cells
US5965424A (en) * 1991-01-11 1999-10-12 Boehringer Mannheim Gmbh Methods for making neisseria or hemophilus IgA protease and DNA encoding the proteases
EP0575553B1 (en) * 1991-03-14 1998-12-16 Imclone Systems, Inc. Recombinant hybrid porin epitopes
FR2681786A1 (en) 1991-09-27 1993-04-02 Centre Nat Rech Scient RECOMBINANT VECTORS OF VIRAL ORIGIN, PROCESS FOR OBTAINING SAME AND THEIR USE FOR THE EXPRESSION OF POLYPEPTIDES IN MUSCLE CELLS.
NZ244306A (en) 1991-09-30 1995-07-26 Boehringer Ingelheim Int Composition for introducing nucleic acid complexes into eucaryotic cells, complex containing nucleic acid and endosomolytic agent, peptide with endosomolytic domain and nucleic acid binding domain and preparation
IL103059A0 (en) 1991-09-30 1993-02-21 Boehringer Ingelheim Int Conjugates for introducing nucleic acid into higher eucaryotic cells
US6100380A (en) 1991-10-28 2000-08-08 Cytran, Inc. Immunomodulating peptides and methods of use
EP0625049A4 (en) 1992-01-23 1995-07-12 Vical Inc Ex vivo gene transfer.
FR2692592B1 (en) * 1992-06-19 1995-03-31 Pasteur Merieux Serums Vacc DNA fragments encoding the Neisseria meningitidis transferrin receptor subunits and methods of expressing them.
EP1498495B1 (en) 1992-07-07 2006-05-31 Fuso Pharmaceutical Industries Ltd. DNA probe specific for Staphylococcus epidermidis
US6592873B1 (en) 1992-10-30 2003-07-15 Iowa State University Research Foundation, Inc. Polynucleic acids isolated from a porcine reproductive and respiratory syndrome virus (PRRSV) and proteins encoded by the polynucleic acids
DK0647655T3 (en) 1993-01-23 1999-08-23 Inmunologia & Genetica Aplic Synthetic peptides and vaccines against parvovirus
US5439808A (en) * 1993-07-23 1995-08-08 North American Vaccine, Inc. Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis
AU7408494A (en) * 1993-07-30 1995-02-28 University Of North Carolina At Chapel Hill, The Production of gonorrheal pi proteins and vaccines in e. coli and salmonella
US5914254A (en) * 1993-08-02 1999-06-22 Celtrix Pharmaceuticals, Inc. Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
US5510264A (en) 1993-09-28 1996-04-23 Insight Biotech Inc. Antibodies which bind meningitis related homologous antigenic sequences
EP0772689B1 (en) 1994-05-09 2007-12-19 Oxford Biomedica (UK) Limited Retroviral vectors having a reduced recombination rate
FR2720408B1 (en) * 1994-05-31 1996-08-14 Pasteur Merieux Serums Vacc Fragments Tbp2 of Neisseria meningitidis.
US6245337B1 (en) 1994-08-25 2001-06-12 Washington University Haemophilus adherence and penetration proteins
IL117483A (en) 1995-03-17 2008-03-20 Bernard Brodeur Proteinase k resistant surface protein of neisseria meningitidis
US5646259A (en) 1995-03-24 1997-07-08 St. Louis University DNA encoding haemophilus adhesion proteins
US6265567B1 (en) 1995-04-07 2001-07-24 University Of North Carolina At Chapel Hill Isolated FrpB nucleic acid molecule
AU721954B2 (en) * 1995-06-07 2000-07-20 Connaught Laboratories Inc. Expression of lipoproteins
CA2232410C (en) 1995-09-18 2003-06-17 United States Army Medical Research Materiel Command (Usamrmc) Improved methods for the production of non-covalently complexed and multivalent proteosome sub-unit vaccines
DE19534579C2 (en) 1995-09-18 2000-06-08 Max Planck Gesellschaft Nucleic acid molecules encoding proteins that mediate the adhesion of Neisseria cells to human cells
FR2739624B1 (en) * 1995-10-10 1997-12-05 Pasteur Merieux Serums Vacc NEW NEISSERIA MENINGITIDIS TBP2 SUBUNIT
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
KR19990082265A (en) * 1996-02-01 1999-11-25 다니엘 제이. 압둔-나비 Method and vaccine for expressing group B Neisseria meningitidis outer membrane (MB3) protein in yeast
US6083499A (en) 1996-04-19 2000-07-04 Mycogen Corporation Pesticidal toxins
WO1998017805A2 (en) 1996-10-24 1998-04-30 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES, c/o Centers for Disease Control and Prevention, Technology Transfer Office Invasion associated genes from neisseria meningitidis serogroup b
US5980898A (en) 1996-11-14 1999-11-09 The United States Of America As Represented By The U.S. Army Medical Research & Material Command Adjuvant for transcutaneous immunization
EP0948625B1 (en) 1996-12-20 2011-01-26 The Board Of Regents, The University Of Texas System Uspa1 and uspa2 antigens of moraxella catarrhalis
US6017531A (en) 1997-06-02 2000-01-25 W. R. Grace & Co. Hydrophilic composition containing protease produced by Vibrio
US6583275B1 (en) 1997-07-02 2003-06-24 Genome Therapeutics Corporation Nucleic acid sequences and expression system relating to Enterococcus faecium for diagnostics and therapeutics
US6914131B1 (en) * 1998-10-09 2005-07-05 Chiron S.R.L. Neisserial antigens
CA2308606A1 (en) 1997-11-06 1999-05-20 Chiron S.P.A. Neisserial antigens
GB9726398D0 (en) 1997-12-12 1998-02-11 Isis Innovation Polypeptide and coding sequences
DE69941567D1 (en) * 1998-01-14 2009-12-03 Novartis Vaccines & Diagnostic ANTIGENE FROM NEISSERIA MENINGITIDIS
BR9909097A (en) * 1998-02-03 2000-12-05 Ct Disease Contr & Prevention Hybrid nucleic acid molecule, expression vector, processes for preparation and for the production of recombinant lipid psa protein, recombinantly produced isolated and purified lipid protein, immunological composition, processes to induce an immune response in an animal and to immunize a host against pneumococcal infection, and, immunogenic composition for intranasal administration to a host
HUP0100869A3 (en) * 1998-02-11 2002-12-28 Lilly Co Eli Processes and intermediates useful to make antifolates
GB9808734D0 (en) 1998-04-23 1998-06-24 Smithkline Beecham Biolog Novel compounds
GB9808866D0 (en) 1998-04-24 1998-06-24 Smithkline Beecham Biolog Novel compounds
US6150502A (en) 1998-04-29 2000-11-21 Genesis Research & Development Corporation Limited Polypeptides expressed in skin cells
US6615024B1 (en) * 1998-05-01 2003-09-02 Arraycomm, Inc. Method and apparatus for determining signatures for calibrating a communication station having an antenna array
CN101293920B (en) * 1998-05-01 2012-07-18 诺华疫苗和诊断公司 Neisseria meningitidis antigens and compositions
US20070026021A1 (en) 1998-05-01 2007-02-01 Chiron S.R.I. Neisseria meningitidis antigens and compositions
US5990085A (en) * 1998-05-04 1999-11-23 Michigan State University Inhibin-HBc fusion protein
GB9810276D0 (en) 1998-05-13 1998-07-15 Smithkline Beecham Biolog Novel compounds
US6248329B1 (en) * 1998-06-01 2001-06-19 Ramaswamy Chandrashekar Parasitic helminth cuticlin nucleic acid molecules and uses thereof
EP1144998A3 (en) * 1998-10-09 2002-08-07 Chiron Corporation Neisseria genomic sequences and methods of their use
WO2000042192A1 (en) 1999-01-15 2000-07-20 Smithkline Beecham Biologicals S.A. Neisseria meningitidis polypeptide basb052
AU2292800A (en) 1999-01-22 2000-08-07 Smithkline Beecham Biologicals (Sa) Neisseria meningitidis antigenic polypeptides, corresponding polynucleotides andprotective antibodies
GB9902084D0 (en) 1999-01-29 1999-03-24 Smithkline Beecham Biolog Novel compounds
ES2228454T3 (en) * 1999-02-26 2005-04-16 Chiron S.R.L. IMPROVEMENT OF THE BACTERIAL ACTIVITY OF NEISSERIA ANTIGENS WITH OLIGONUCLEOTIDES CONTAINING MOTIVES CG.
WO2000066791A1 (en) 1999-04-30 2000-11-09 Chiron Corporation Neisseria genomic sequences and methods of their use
NZ530640A (en) 1999-04-30 2006-06-30 Chiron S Conserved neisserial antigens
GB9911683D0 (en) 1999-05-19 1999-07-21 Chiron Spa Antigenic peptides
CA2929348A1 (en) 1999-05-19 2000-11-30 Novartis Vaccines And Diagnostics S.R.L. Combination neisserial compositions
GB9916529D0 (en) * 1999-07-14 1999-09-15 Chiron Spa Antigenic peptides
AU784203B2 (en) * 1999-10-29 2006-02-23 Glaxosmithkline Biologicals S.A. Neisserial antigenic peptides
AU1875301A (en) * 1999-11-29 2001-06-04 Chiron S.P.A. 85kda neisserial antigen
GB9928676D0 (en) 1999-12-03 2000-02-02 Provalis Uk Ltd Pseudomonas aeruginosa antigens
US20010031268A1 (en) * 1999-12-17 2001-10-18 Baldwin Thomas John Antigen preparations
RU2279889C2 (en) 2000-01-17 2006-07-20 Чирон С.Р.Л. OUTER MEMBRANE VESICLE VACCINE (OMV) CONTAINING PROTEINS OF SEROGROUP B N.Meningitis OUTER MEMBRANE
PL216780B1 (en) 2000-01-25 2014-05-30 Univ Queensland Of Santa Lucia PROTEINS COMPRISING CONSERVED REGIONS OF NEISSERIA MENINGITIDIS SURFACE ANTIGEN NhhA
ES2391153T3 (en) * 2000-02-28 2012-11-22 Novartis Vaccines And Diagnostics S.R.L. Heterologous expression of Neisseria proteins
CA2407352A1 (en) 2000-04-21 2001-11-01 Corixa Corporation Compositions and methods for the therapy and diagnosis of acne vulgaris
EP1297005B1 (en) 2000-07-03 2009-08-26 Novartis Vaccines and Diagnostics S.r.l. Immunisation against chlamydia pneumoniae
GB0017149D0 (en) 2000-07-12 2000-08-30 Chiron Spa Helicobacter pylori mutants
GB0103170D0 (en) 2001-02-08 2001-03-28 Smithkline Beecham Biolog Vaccine composition
US20040073294A1 (en) * 2002-09-20 2004-04-15 Conor Medsystems, Inc. Method and apparatus for loading a beneficial agent into an expandable medical device
GB0103424D0 (en) * 2001-02-12 2001-03-28 Chiron Spa Gonococcus proteins
WO2003009869A1 (en) 2001-07-26 2003-02-06 Chiron Srl. Vaccines comprising aluminium adjuvants and histidine
GB0118249D0 (en) 2001-07-26 2001-09-19 Chiron Spa Histidine vaccines
GB0121591D0 (en) * 2001-09-06 2001-10-24 Chiron Spa Hybrid and tandem expression of neisserial proteins
DK2248822T3 (en) 2001-07-27 2017-02-13 Glaxosmithkline Biologicals Sa MENINGOCOKKER ADHESIONS
MX339524B (en) 2001-10-11 2016-05-30 Wyeth Corp Novel immunogenic compositions for the prevention and treatment of meningococcal disease.
GB0129007D0 (en) * 2001-12-04 2002-01-23 Chiron Spa Adjuvanted antigenic meningococcal compositions
EP1961427A3 (en) 2002-08-02 2009-11-04 GlaxoSmithKline Biologicals S.A. Neisserial vaccine compositions comprising a combination of antigens
US7785608B2 (en) 2002-08-30 2010-08-31 Wyeth Holdings Corporation Immunogenic compositions for the prevention and treatment of meningococcal disease
DE60335477D1 (en) * 2002-10-11 2011-02-03 Novartis Vaccines & Diagnostic POLYPEPTIDE FOR WIDE PROTECTION AGAINST HYPERVIRULENT MENINGOKOKKIN LINES
GB0227346D0 (en) 2002-11-22 2002-12-31 Chiron Spa 741
CA2512917A1 (en) 2003-01-15 2004-08-05 Wyeth Holdings Corporation Methods for increasing neisseria protein expression
EP2172213B1 (en) * 2003-01-30 2013-04-03 Novartis AG Injectable vaccines against multiple meningococcal serogroups
WO2004094596A2 (en) 2003-04-16 2004-11-04 Wyeth Holdings Corporation Novel immunogenic compositions for the prevention and treatment of meningococcal disease
GB0315021D0 (en) * 2003-06-26 2003-07-30 Chiron Srl Immunogenic gonococcal compositions
GB0323103D0 (en) * 2003-10-02 2003-11-05 Chiron Srl De-acetylated saccharides
PL1961426T3 (en) * 2003-10-02 2012-03-30 Gsk Vaccines S R L Combined meningitis vaccines
GB0408977D0 (en) * 2004-04-22 2004-05-26 Chiron Srl Immunising against meningococcal serogroup Y using proteins
GB0409748D0 (en) * 2004-04-30 2004-06-09 Chiron Srl Lactoferrin cleavage
GB0410866D0 (en) * 2004-05-14 2004-06-16 Chiron Srl Haemophilius influenzae
GB0419408D0 (en) * 2004-09-01 2004-10-06 Chiron Srl 741 chimeric polypeptides
JP4993750B2 (en) 2005-01-27 2012-08-08 チルドレンズ ホスピタル アンド リサーチ センター アット オークランド Vesicular vaccine based on GNA1870 for broad protection against diseases caused by Neisseria meningitidis
GB0524066D0 (en) * 2005-11-25 2006-01-04 Chiron Srl 741 ii
ES2670231T3 (en) 2006-03-22 2018-05-29 Glaxosmithkline Biologicals S.A. Regimens for immunization with meningococcal conjugates
TW200806315A (en) 2006-04-26 2008-02-01 Wyeth Corp Novel formulations which stabilize and inhibit precipitation of immunogenic compositions
BRPI0713904A2 (en) * 2006-06-29 2013-06-25 Novartis Ag polypeptides from neisseria meningitidis
AR064642A1 (en) * 2006-12-22 2009-04-15 Wyeth Corp POLINUCLEOTIDE VECTOR THAT INCLUDES IT RECOMBINATING CELL THAT UNDERSTANDS THE VECTOR POLYPEPTIDE, ANTIBODY, COMPOSITION THAT UNDERSTANDS THE POLINUCLEOTIDE, VECTOR, RECOMBINATING CELL POLYPEPTIDE OR ANTIBODY, USE OF THE COMPOSITION AND A COMPOSITION AND A METHOD
US20100150912A1 (en) 2007-04-11 2010-06-17 Novartis Ag Blocking interaction between pathogen factors and factor h to inhibit hemorrhagic syndromes
AU2008259423A1 (en) 2007-06-04 2008-12-11 Novartis Ag Formulation of meningitis vaccines
AU2009215364B2 (en) 2008-02-21 2014-09-18 Glaxosmithkline Biologicals S.A. Meningococcal fHBP polypeptides
MY150481A (en) * 2008-03-03 2014-01-30 Irm Llc Compounds and compositions as tlr activity modulators
ES2557282T3 (en) 2008-03-10 2016-01-25 Children's Hospital & Research Center At Oakland Chimeric H-factor binding proteins (fHBP) containing a heterologous B domain, and methods of use
CA2733943A1 (en) 2008-09-03 2010-03-11 Children's Hospital & Research Center At Oakland Peptides presenting an epitope of an a domain of factor h binding protein and methods of use
IT1394288B1 (en) 2008-09-12 2012-06-06 Novartis Vaccines & Diagnostic PROTEIN IMMUNOGENES THAT LINK THE FACTOR H.
GB0819633D0 (en) 2008-10-25 2008-12-03 Isis Innovation Composition
CA2756522C (en) 2009-03-24 2018-06-26 Novartis Ag Adjuvanting meningococcal factor h binding protein
EP2719395A1 (en) * 2010-09-01 2014-04-16 Novartis AG Adsorption of immunopotentiators to insoluble metal salts
ES2744471T3 (en) * 2010-09-04 2020-02-25 Glaxosmithkline Biologicals Sa Bactericidal antibody assays to assess immunogenesis and potency of meningococcal capsular saccharide vaccines
AU2011300418B2 (en) * 2010-09-10 2016-05-12 Glaxosmithkline Biologicals Sa Meningococcus overexpressing NadA and/or NHBA and outer membrane vesicles derived therefrom
US9499593B2 (en) * 2011-05-11 2016-11-22 Children's Medical Center Corporation Modified biotin-binding protein, fusion proteins thereof and applications
US9184727B2 (en) * 2012-06-11 2015-11-10 Phonon Corporation SAW device and method for post-seal frequency trimming

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