CA2392722C - Region regulant de preference les tissus males et methode d'utilisation - Google Patents

Region regulant de preference les tissus males et methode d'utilisation Download PDF

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Publication number
CA2392722C
CA2392722C CA2392722A CA2392722A CA2392722C CA 2392722 C CA2392722 C CA 2392722C CA 2392722 A CA2392722 A CA 2392722A CA 2392722 A CA2392722 A CA 2392722A CA 2392722 C CA2392722 C CA 2392722C
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CA
Canada
Prior art keywords
plant
male
nucleic acid
seq
base
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2392722A
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English (en)
Other versions
CA2392722A1 (fr
Inventor
Marc C. Albertsen
Timothy W. Fox
Carl W. Garnaat
Gary Huffman
Timmy L. Kendall
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Pioneer Hi Bred International Inc
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Pioneer Hi Bred International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Pioneer Hi Bred International Inc filed Critical Pioneer Hi Bred International Inc
Publication of CA2392722A1 publication Critical patent/CA2392722A1/fr
Application granted granted Critical
Publication of CA2392722C publication Critical patent/CA2392722C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne une séquence nucléotidique isolée codant la région régulatrice préférée des tissus mâles Ms45. Dans un aspect, cette invention concerne l'utilisation de cette région régulatrice préférée des tissus mâles pour induire une fertilité. Un exemple de cette utilisation est la production d'une semence hybride telle que dans un système de stérilité mâle. La région régulatrice préférée des tissus mâles Ms45 peut être liée fonctionnellement à des gènes exogènes, tels que ceux codant des cytotoxines, des unités nucléotidiques complémentaires et des molécules inhibitrices. Cette invention concernent également des cellules de plantes, des tissus de plantes ainsi que des plantes différenciées contenant la région régulatrice de cette invention.
CA2392722A 2000-02-15 2001-02-13 Region regulant de preference les tissus males et methode d'utilisation Expired - Lifetime CA2392722C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US50448700A 2000-02-15 2000-02-15
US09/504,487 2000-02-15
PCT/US2001/004527 WO2001060997A2 (fr) 2000-02-15 2001-02-13 Region regulatrice preferee des tissus males et son procede d'utilisation

Publications (2)

Publication Number Publication Date
CA2392722A1 CA2392722A1 (fr) 2001-08-23
CA2392722C true CA2392722C (fr) 2010-05-11

Family

ID=24006485

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2392722A Expired - Lifetime CA2392722C (fr) 2000-02-15 2001-02-13 Region regulant de preference les tissus males et methode d'utilisation

Country Status (6)

Country Link
EP (1) EP1255825A2 (fr)
AU (1) AU3817701A (fr)
CA (1) CA2392722C (fr)
HU (1) HUP0301053A3 (fr)
NZ (1) NZ520704A (fr)
WO (1) WO2001060997A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070169227A1 (en) 2003-12-16 2007-07-19 Pioneer Hi-Bred International Inc. Dominant Gene Suppression Transgenes and Methods of Using Same
CN104703998B (zh) * 2012-03-13 2020-08-21 先锋国际良种公司 植物中雄性育性的遗传减少
MX2014011043A (es) 2012-03-13 2015-06-02 Pioneer Hi Bred Int Reduccion genetica de la fertilidad masculina en plantas.
WO2013138354A1 (fr) 2012-03-13 2013-09-19 Pioneer Hi-Bred International, Inc. Réduction génétique de la fertilité mâle chez les plantes
US9803214B2 (en) 2013-03-12 2017-10-31 Pioneer Hi-Bred International, Inc. Breeding pair of wheat plants comprising an MS45 promoter inverted repeat that confers male sterility and a construct that restores fertility

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5837850A (en) * 1994-04-21 1998-11-17 Pioneer Hi-Bred International, Inc. Regulatory element conferring tapetum specificity
US5763243A (en) * 1994-12-08 1998-06-09 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
US6037523A (en) * 1997-06-23 2000-03-14 Pioneer Hi-Bred International Male tissue-preferred regulatory region and method of using same

Also Published As

Publication number Publication date
EP1255825A2 (fr) 2002-11-13
WO2001060997A2 (fr) 2001-08-23
WO2001060997A3 (fr) 2002-03-07
HUP0301053A3 (en) 2004-10-28
CA2392722A1 (fr) 2001-08-23
HUP0301053A2 (hu) 2003-08-28
NZ520704A (en) 2005-05-27
AU3817701A (en) 2001-08-27

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Effective date: 20210215