CA2391571A1 - Procede d'identification rapide et precise de micro-organismes - Google Patents

Procede d'identification rapide et precise de micro-organismes Download PDF

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Publication number
CA2391571A1
CA2391571A1 CA002391571A CA2391571A CA2391571A1 CA 2391571 A1 CA2391571 A1 CA 2391571A1 CA 002391571 A CA002391571 A CA 002391571A CA 2391571 A CA2391571 A CA 2391571A CA 2391571 A1 CA2391571 A1 CA 2391571A1
Authority
CA
Canada
Prior art keywords
primer
target sequence
sequence
oligonucleotide
organism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002391571A
Other languages
English (en)
Inventor
Zhiping Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Apollo Biotechnology Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2391571A1 publication Critical patent/CA2391571A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés et des compositions utilisés pour l'identification rapide de micro-organismes. L'invention concerne également des procédés et des compositions servant à l'identification rapide et simultanée dans un échantillon biologique de multiples micro-organismes, comportant des bactéries, des champignons, des virus et de la levure. L'invention fait également état de procédés et de compositions employant de techniques d'amplification et d'hybridation de séquence spécifique, afin de détecter d'une séquence de polynucléotide spécifique d'une espèce dans un échantillon. L'invention présente aussi de nouveaux procédés associant des sondes d'oligonucléotides à des surfaces en verre à l'efficacité améliorée.
CA002391571A 1999-11-16 2000-11-16 Procede d'identification rapide et precise de micro-organismes Abandoned CA2391571A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US16588199P 1999-11-16 1999-11-16
US60/165,881 1999-11-16
US47945700A 2000-01-06 2000-01-06
US09/479,457 2000-01-06
PCT/US2000/031579 WO2001036683A2 (fr) 1999-11-16 2000-11-16 Procede d'identification rapide et precise de micro-organismes

Publications (1)

Publication Number Publication Date
CA2391571A1 true CA2391571A1 (fr) 2001-05-25

Family

ID=26861776

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002391571A Abandoned CA2391571A1 (fr) 1999-11-16 2000-11-16 Procede d'identification rapide et precise de micro-organismes

Country Status (4)

Country Link
EP (1) EP1261716A2 (fr)
AU (1) AU1771901A (fr)
CA (1) CA2391571A1 (fr)
WO (1) WO2001036683A2 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1217009C (zh) * 2001-09-01 2005-08-31 三星电子株式会社 利用具有环氧基的星状聚乙二醇衍生物制备水凝胶生物芯片的方法
JP2003144153A (ja) * 2001-11-09 2003-05-20 Gifu Univ 遺伝子検出方法、遺伝子検出用プライマー、ｄｎａマイクロアレイ及び遺伝子検出用キット
US20050202414A1 (en) * 2001-11-15 2005-09-15 The Regents Of The University Of California Apparatus and methods for detecting a microbe in a sample
US6881835B2 (en) * 2002-01-04 2005-04-19 Dr. Chip Biotechnology Inc. Detection of respiratory viruses
DE10244456A1 (de) * 2002-09-24 2004-04-15 Hain Lifescience Gmbh Verfahren zum Nachweis und Differenzierung von Bakterien
FI113549B (fi) * 2002-11-19 2004-05-14 Mobidiag Oy Diagnostinen menetelmä hengitystieinfektioita aiheuttavien bakteerien osoittamiseksi ja tunnistamiseksi ja menetelmässä käyttökelpoinen alukeseos
FR2860801B1 (fr) * 2003-10-10 2007-09-21 Bertin Technologies Sa Methode de detection rapide de micro-organismes sur puces a adn
EP1815016B1 (fr) * 2004-11-01 2012-06-27 George Mason University Compositions et methodes pour le diagnostic de troubles du colon
RU2348695C2 (ru) * 2006-05-23 2009-03-10 Закрытое акционерное общество "Молекулярно-медицинские технологии" Дифференцирующий и специфический олигонуклеотиды для идентификации последовательностей днк инфекционных агентов в биологических материалах, способ видовой идентификации инфекционных агентов, биочип и набор для осуществления этого способа
JP5596894B2 (ja) * 2006-11-10 2014-09-24 キヤノン株式会社 プローブセット、プローブ固定担体及び遺伝子検査方法
EP2539467B1 (fr) * 2010-02-24 2016-06-08 The Broad Institute, Inc. Procédés de diagnostic de pathogènes de maladies infectieuses et leur sensibilité aux médicaments
GB201314873D0 (en) * 2013-08-20 2013-10-02 Univ Cape Town Method for diagnosing tuberculosis in a urine sample
WO2018185105A1 (fr) * 2017-04-03 2018-10-11 Bio-Me As Procédés de détection de micro-organismes

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4992172A (en) * 1987-09-14 1991-02-12 Gelman Sciences, Inc. Blotting methods using polyaldehyde activated membranes
US5708160A (en) * 1995-04-26 1998-01-13 The National Research Council HSP-60 genomic locus and primers for species identification
US5994066A (en) * 1995-09-11 1999-11-30 Infectio Diagnostic, Inc. Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
WO1998028444A2 (fr) * 1996-12-23 1998-07-02 The University Of Chicago Micropuces oligonucleotidiques sur mesure utilisees comme capteurs multiples
DE19716456C2 (de) * 1997-04-21 2002-05-08 Schmitt Heinz Josef Verwendung einer Kombination von Primern zum Nachweis von Erregern von Infektionskrankheiten
US6159949A (en) * 1997-08-29 2000-12-12 Smithkline Beecham Corporation FtsY of Staphyloccus aureus

Also Published As

Publication number Publication date
WO2001036683A3 (fr) 2002-09-26
WO2001036683A2 (fr) 2001-05-25
EP1261716A2 (fr) 2002-12-04
AU1771901A (en) 2001-05-30

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