CA2389390A1 - Enzyme de ramification d'amidon - Google Patents

Enzyme de ramification d'amidon Download PDF

Info

Publication number
CA2389390A1
CA2389390A1 CA002389390A CA2389390A CA2389390A1 CA 2389390 A1 CA2389390 A1 CA 2389390A1 CA 002389390 A CA002389390 A CA 002389390A CA 2389390 A CA2389390 A CA 2389390A CA 2389390 A1 CA2389390 A1 CA 2389390A1
Authority
CA
Canada
Prior art keywords
starch
wheat
endosperm
branching enzyme
plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002389390A
Other languages
English (en)
Inventor
Monica Baga
Ramesh B. Nair
Anne Repellin
Ravindra N. Chibbar
Ming Gao
Pierre Hucl
Mingsheng Peng
Graham J. Scoles
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Council of Canada
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2389390A1 publication Critical patent/CA2389390A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/107—1,4-Alpha-glucan branching enzyme (2.4.1.18)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne une enzyme de ramification d'amidon qui se lie à des granulés d'amidon de type A dans le blé, l'orge, le seigle ou le triticale. Cette enzyme n'est pas sensiblement associée aux granulés d'amidon de type B. Une séquence d'ADNc codant une isoforme de l'enzyme a été isolée du cultivar de blé Fielder, ce qui a permis de déterminer une séquence d'acides aminés dérivée.
CA002389390A 1999-10-29 2000-10-27 Enzyme de ramification d'amidon Abandoned CA2389390A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16214499P 1999-10-29 1999-10-29
US60/162,144 1999-10-29
PCT/CA2000/001276 WO2001032886A2 (fr) 1999-10-29 2000-10-27 Enzyme de ramification d'amidon

Publications (1)

Publication Number Publication Date
CA2389390A1 true CA2389390A1 (fr) 2001-05-10

Family

ID=22584342

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002389390A Abandoned CA2389390A1 (fr) 1999-10-29 2000-10-27 Enzyme de ramification d'amidon

Country Status (3)

Country Link
AU (1) AU1122701A (fr)
CA (1) CA2389390A1 (fr)
WO (1) WO2001032886A2 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPQ005299A0 (en) 1999-04-29 1999-05-27 Commonwealth Scientific And Industrial Research Organisation Novel genes encoding wheat starch synthases and uses therefor
DK1331845T3 (da) 2000-11-09 2012-04-23 Commw Scient Ind Res Org Byg med reduceret SSII-aktivitet og stivelsesholdige produkter med et reduceret indhold af amylopectin
AUPS219802A0 (en) 2002-05-09 2002-06-06 Commonwealth Scientific And Industrial Research Organisation Barley with altered branching enzyme activity and starch and starch containing products with a reduced amylopectin content
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
EP2290084A3 (fr) 2003-06-30 2011-10-12 Commonwealth Scientific and Industrial Research Organization Blé avec activité modifié d'enzyme ramifiant, amidon et produits contenant de l'amidon dérivés dudit blé
KR101274849B1 (ko) 2003-10-27 2013-06-13 코몬웰스 싸이언티픽 엔드 인더스트리얼 리서치 오가니제이션 아밀로스 비율이 증가된 전분을 갖는 쌀 및 이의 생성물
PL1833291T3 (pl) 2004-12-30 2017-05-31 Commonwealth Scientific And Industrial Research Organisation Metoda i środki poprawy stanu zdrowia jelit
US7993686B2 (en) 2004-12-30 2011-08-09 Commonwealth Scientific And Industrial Organisation Method and means for improving bowel health
CA2653883C (fr) 2008-07-17 2022-04-05 Colin Leslie Dow Jenkins Plantes cerealieres a taux eleve de fructosane
MX2012001376A (es) 2009-07-30 2012-09-07 Commw Scient Ind Res Org Cebada y usos de la misma.
NZ610181A (en) 2010-11-04 2015-03-27 Arista Cereal Technologies Pty Ltd High amylose wheat
AU2012212404B2 (en) 2011-02-03 2016-05-12 The Healthy Grain Pty Limited Barley with modified SSIII
BR112014007928B1 (pt) 2011-10-04 2021-07-06 Arcadia Biosciences, Inc. polinucleotídeos sbeiia de trigo
WO2013063653A1 (fr) 2011-11-04 2013-05-10 Arista Cereal Technologies Pty Limited Blé - ii à teneur élevée en amylose

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2217254T3 (es) * 1992-10-14 2004-11-01 Syngenta Limited Nuevas plantas y procesos para obtenerlas.
HUP9902112A3 (en) * 1995-12-20 2001-11-28 Du Pont Novel starches via modification of expression of starch biosynthetic enzyme genes
US6107060A (en) * 1996-09-30 2000-08-22 Exseed Genetics, L.L.C. Starch encapsulation
NZ503137A (en) * 1997-09-12 2000-10-27 Groupe Limagrain Pacific Pty L Sequence and promoters for starch branching enzyme I, starch branching enzyme II, soluble starch synthase I and starch debranching enzyme derived from Triticum tauschii to modulate gene expression

Also Published As

Publication number Publication date
WO2001032886A2 (fr) 2001-05-10
AU1122701A (en) 2001-05-14
WO2001032886A3 (fr) 2001-11-29

Similar Documents

Publication Publication Date Title
Harn et al. Isolation and characterization of the zSSIIa and zSSIIb starch synthase cDNA clones from maize endosperm
Marshall et al. Identification of the major starch synthase in the soluble fraction of potato tubers.
US7041484B1 (en) Starch branching enzymes
Salehuzzaman et al. Isolation and characterization of a cDNA encoding granule-bound starch synthase in cassava (Manihot esculenta Crantz) and its antisense expression in potato
Knight et al. Molecular cloning of starch synthase I from maize (W64) endosperm and expression in Escherichia coli
CA2603919C (fr) Amidon a haute teneur en phosphate
CA2205118C (fr) Molecules d'adn de codage d'enzymes qui participent a la synthese de l'amidon, vecteurs, bacteries, cellules vegetales transgeniques et plantes contenant ces molecules
Abel et al. Cloning and functional analysis of a cDNA encoding a novel 139 kDa starch synthase from potato (Solanum tuberosum L.)
Roitsch et al. Induction of apoplastic invertase of Chenopodium rubrum by D-glucose and a glucose analog and tissue-specific expression suggest a role in sink-source regulation
US8969541B2 (en) Nucleic acids and proteins associated with sucrose accumulation in coffee
Larsson et al. Three isoforms of starch synthase and two isoforms of branching enzyme are present in potato tuber starch
Ji et al. Microbial starch-binding domains as a tool for targeting proteins to granules during starch biosynthesis
Ainsworth et al. Adenosine diphosphate glucose pyrophosphorylase genes in wheat: differential expression and gene mapping
CA2389390A1 (fr) Enzyme de ramification d'amidon
JP2007525987A (ja) 複数のデンプンリン酸化酵素の活性が増大した植物
Veramendi et al. Potato hexokinase 2 complements transgenic Arabidopsis plants deficient in hexokinase 1 but does not play a key role in tuber carbohydrate metabolism
US20100154074A1 (en) Nucleic Acids and Proteins Associated with Sucrose Degradation in Coffee
Edwards et al. Evidence that a 77-kilodalton protein from the starch of pea embryos is an isoform of starch synthase that is both soluble and granule bound
AU2006304484A1 (en) Nucleic acids and proteins associated with galactomannan synthesis in coffee
Peng et al. Isolation, characterization and expression analysis of starch synthase I from wheat (Triticum aestivum L.)
CA2284688C (fr) Pullulanase du mais
US6323015B1 (en) Sucrose phosphate synthase
CA2455200A1 (fr) Modification d'amidon
Repellin et al. Characterization of a cDNA encoding a type I starch branching enzyme produced in developing wheat (Triticum aestivum L.) kernels
US6756218B2 (en) Sucrose phosphate synthase

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued