CA2388982A1 - Criblages a haut rendement bases sur des plaques de microtitration - Google Patents

Criblages a haut rendement bases sur des plaques de microtitration Download PDF

Info

Publication number
CA2388982A1
CA2388982A1 CA002388982A CA2388982A CA2388982A1 CA 2388982 A1 CA2388982 A1 CA 2388982A1 CA 002388982 A CA002388982 A CA 002388982A CA 2388982 A CA2388982 A CA 2388982A CA 2388982 A1 CA2388982 A1 CA 2388982A1
Authority
CA
Canada
Prior art keywords
molecule
cell
dna
interest
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002388982A
Other languages
English (en)
Inventor
Henrik Pedersen
Michael Lamsa
Peter Kamp Hansen
Henrik Frisner
Jesper Vind
Steffen Ernst
Lars Kongsbak
Birthe Ravn Joergensen
Thomas Christian Beck
Tommy Lykke Husum
Ingemar Von Ossowski
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2388982A1 publication Critical patent/CA2388982A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)

Abstract

La présente invention concerne une méthode permettant de réaliser des analyses destinées au criblage efficace et précis de grands nombres de populations cellulaires produisant des variants d'une molécule considérée. Selon un premier aspect, l'invention concerne une méthode de criblage à haut rendement réalisée sur une population importante de cellules hôtes et visant à détecter la production d'une molécule considérée, laquelle méthode consiste: a) à disposer les cellules hôtes selon un certain agencement spatial, de manière que chaque position dudit agencement spatial soit occupée par une seule cellule; b) à cultiver les cellules hôtes dans des conditions de croissance se prêtant à un criblage à haut rendement; c) à analyser chaque position de l'agencement pour y détecter la production de la molécule considérée; et d) à sélectionner les cellules provenant des positions où ladite molécule a été produite, tel que déterminé en c).
CA002388982A 1999-11-05 2000-10-10 Criblages a haut rendement bases sur des plaques de microtitration Abandoned CA2388982A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA199901605 1999-11-05
DKPA199901605 1999-11-05
PCT/DK2000/000568 WO2001032844A1 (fr) 1999-11-05 2000-10-10 Criblages a haut rendement bases sur des plaques de microtitration

Publications (1)

Publication Number Publication Date
CA2388982A1 true CA2388982A1 (fr) 2001-05-10

Family

ID=8106475

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002388982A Abandoned CA2388982A1 (fr) 1999-11-05 2000-10-10 Criblages a haut rendement bases sur des plaques de microtitration

Country Status (4)

Country Link
EP (1) EP1230348A1 (fr)
AU (1) AU7646200A (fr)
CA (1) CA2388982A1 (fr)
WO (1) WO2001032844A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7647184B2 (en) * 2001-08-27 2010-01-12 Hanall Pharmaceuticals, Co. Ltd High throughput directed evolution by rational mutagenesis
AU2002361220A1 (en) * 2001-12-31 2003-07-15 Institut Fur Physikalische Hochtechnologie E.V. Micro-recess array for size-dependent sorting or separation of cells suspended in a flowing medium and method for analysing the functional activity of individual cells
AU2003263552A1 (en) 2002-09-09 2004-03-29 Nautilus Biotech Rational evolution of cytokines for higher stability, the cytokines and encoding nucleic acid molecules
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
WO2004090549A1 (fr) * 2003-04-11 2004-10-21 Novozymes A/S Procede de criblage pour des polypeptides glycosyles secretes
EP1477805A1 (fr) 2003-05-12 2004-11-17 ProBioGen AG Procédé de sélection de cellules
WO2005017138A2 (fr) * 2003-08-19 2005-02-24 Novozymes A/S Titration des sites actifs de gylcosyle hydrolases
CA2574953A1 (fr) 2004-07-26 2006-02-09 Dow Global Technolgies Inc. Procede permettant d'ameliorer l'expression d'une proteine par mise au point d'une souche par genie genetique
US7655421B2 (en) * 2005-06-17 2010-02-02 Ciencia, Inc. Cytometer on a chip
US9580719B2 (en) * 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
CN101688213A (zh) * 2007-04-27 2010-03-31 陶氏环球技术公司 用于快速筛选微生物宿主以鉴定某些在表达异源蛋白质方面具有改善的产量和/或质量的菌株的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5654150A (en) * 1995-06-07 1997-08-05 President And Fellows Of Harvard College Method of expression cloning
US6235520B1 (en) * 1996-06-27 2001-05-22 Cellstat Technologies, Inc. High-throughput screening method and apparatus
WO1999055826A1 (fr) * 1998-04-24 1999-11-04 Genova Pharmaceuticals Corporation Dispositif de micro-compartimentalisation et ses utilisations
WO1999060170A1 (fr) * 1998-05-21 1999-11-25 Hyseq, Inc. Reseaux lineaires de composes immobilises et procedes d'utilisation de ces derniers

Also Published As

Publication number Publication date
AU7646200A (en) 2001-05-14
EP1230348A1 (fr) 2002-08-14
WO2001032844A1 (fr) 2001-05-10

Similar Documents

Publication Publication Date Title
US7794962B2 (en) High-throughput screening of expressed DNA libraries in filamentous fungi
US8569029B2 (en) DNase expression in recombinant host cells
EP1276857B1 (fr) Piegeage de sequences de signaux
US9045748B2 (en) Methods for transforming and expression screening of filamentous fungal cells with a DNA library
CA2388982A1 (fr) Criblages a haut rendement bases sur des plaques de microtitration
JP2005514911A6 (ja) 転写調節用ｄｎａ配列
JP2005514911A (ja) 転写調節用ｄｎａ配列
US20080058214A1 (en) Signal sequence trapping
JP5138855B2 (ja) 糸状菌において発現されるｄｎａライブラリーのハイスループットスクリーニング
JP3910634B2 (ja) Ｐｆ１０２２物質を産生する形質転換体、及び糸状菌綱に属する菌の形質転換方法
WO2001090393A1 (fr) Procede pour augmenter le nombre de copies de gene dans une cellule hote et cellule hote resultante
WO2001032829A2 (fr) Procedes permettant de cribler des micro-organismes ou des banques de genes pour des produits secretes par une cellule
CN107667177A (zh) 用于选择具有增强活性的酶的方法
EP2078078B1 (fr) Selection de geènes synthetiques bien exprimes
WO2001032858A1 (fr) Procede de criblage a haut debit (hts)
CA2425380A1 (fr) Plantes transgeniques
KR100825290B1 (ko) 염색체내 형광단백질 유전자 일부가 삽입된 세포를포함하는 이분자 형광 상보 시스템 및 이를 이용한 이분자형광 상보 기법
CN111778270B (zh) 通过整合发光报告基因反映体外无细胞蛋白表达水平的方法
WO2001032834A1 (fr) Procede de criblage de populations de cellules
AU779195B2 (en) High throughput screening (HTS) assays for protein variants with reduced antibody binding capacity
US20030041354A1 (en) Transgenic plants
WO2001049878A1 (fr) BETA-GALACTOSIDASES Fam35 FONGIQUES ET EXTRACELLULAIRES
WO2005024012A1 (fr) Expression accrue d'un polypeptide modifie
CN111778238A (zh) 一种基于蛋白互作原理的编码线粒体磷转运蛋白基因的筛选方法
US20110045482A1 (en) Screening methods for identifying target antifungal genes and compounds by detecting cell surface glycoproteins

Legal Events

Date Code Title Description
FZDE Dead