CA2385353A1 - Manipulation transgenique de sn-glycerol-3-phosphate et production de glycerol avec un gene de glycerol-3-phosphate deshydrogenase a retroaction deficiente - Google Patents

Manipulation transgenique de sn-glycerol-3-phosphate et production de glycerol avec un gene de glycerol-3-phosphate deshydrogenase a retroaction deficiente Download PDF

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Publication number
CA2385353A1
CA2385353A1 CA002385353A CA2385353A CA2385353A1 CA 2385353 A1 CA2385353 A1 CA 2385353A1 CA 002385353 A CA002385353 A CA 002385353A CA 2385353 A CA2385353 A CA 2385353A CA 2385353 A1 CA2385353 A1 CA 2385353A1
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Canada
Prior art keywords
plant
glycerol
phosphate dehydrogenase
seq
dna sequence
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Granted
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CA002385353A
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English (en)
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CA2385353C (fr
Inventor
Jitao Zou
Yangdou Wei
Cyril Periappuram
Gopalan Selvaraj
Raju Datla
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National Research Council of Canada
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Publication of CA2385353A1 publication Critical patent/CA2385353A1/fr
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Publication of CA2385353C publication Critical patent/CA2385353C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20—Glycerol

Abstract

La présente invention concerne un procédé qui permet de transformer génétiquement une plante de manière à ce qu'elle exprime une glycérol-3-phosphate déshydrogénase à rétroaction déficiente. L'enzyme à rétroaction déficiente augmente les taux de glycérol et de glycérol-3-phosphate par rapport au type sauvage, ceci induisant une meilleure tolérance au choc osmotique, et une quantité réduite d'acides gras dans les glycérolipides.
CA2385353A 1999-09-22 2000-09-21 Manipulation transgenique de sn-glycerol-3-phosphate et production de glycerol avec un gene de glycerol-3-phosphate deshydrogenase a retroaction deficiente Expired - Fee Related CA2385353C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15513399P 1999-09-22 1999-09-22
US60/155,133 1999-09-22
PCT/CA2000/001096 WO2001021820A1 (fr) 1999-09-22 2000-09-21 Manipulation transgenique de sn-glycerol-3-phosphate et production de glycerol avec un gene de glycerol-3-phosphate deshydrogenase a retroaction deficiente

Publications (2)

Publication Number Publication Date
CA2385353A1 true CA2385353A1 (fr) 2001-03-29
CA2385353C CA2385353C (fr) 2010-04-20

Family

ID=22554224

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2385353A Expired - Fee Related CA2385353C (fr) 1999-09-22 2000-09-21 Manipulation transgenique de sn-glycerol-3-phosphate et production de glycerol avec un gene de glycerol-3-phosphate deshydrogenase a retroaction deficiente

Country Status (5)

Country Link
US (1) US7112724B1 (fr)
EP (1) EP1214432A1 (fr)
AU (1) AU7399100A (fr)
CA (1) CA2385353C (fr)
WO (1) WO2001021820A1 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7759547B2 (en) * 1999-09-22 2010-07-20 National Research Council Of Canada Methods of producing and growing plants having improved phosphorus utilization
US7579517B2 (en) 2002-05-08 2009-08-25 Basf Plant Science Gmbh Methods for increasing oil content in plants
GB0301415D0 (en) * 2003-01-22 2003-02-19 Univ York Glycerol kinase
US8207398B2 (en) * 2005-07-18 2012-06-26 University Of Kentucky Research Foundation Plants having an enhanced resistance to necrotrophic pathogens and method of making same
DE102005053318A1 (de) * 2005-11-07 2007-05-10 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren zur Erhöhung des Gesamtölgehaltes in Ölpflanzen
US7732155B2 (en) 2006-12-13 2010-06-08 National Research Council Of Canada Methods for identifying lysophosphatidylcholine acyltransferases
EP2234474B1 (fr) 2007-12-21 2016-10-26 National Research Council of Canada Gènes de diacylglycérol acyltransférase 2 et protéines qu'ils codent, issus d'algues
US20110093983A1 (en) * 2008-03-26 2011-04-21 National Research Council Of Canada Algal glycerol-3 phosphate acyltransferase
US8461417B2 (en) * 2008-05-21 2013-06-11 National Research Council Of Canada Reduction of lyso-phosphatidylcholine acyltransferase activity
CN103911386B (zh) * 2014-04-16 2016-05-11 上海市农业生物基因中心 提高植物的胁迫耐性的人工融合基因
JP6587468B2 (ja) 2015-09-11 2019-10-09 花王株式会社 脂質の製造方法
CN110684749A (zh) * 2019-11-13 2020-01-14 黑龙江八一农垦大学 玉米3-磷酸甘油脱氢酶ZmGPDH4及其编码基因在调控植物耐逆性中的应用
WO2023280175A1 (fr) * 2021-07-06 2023-01-12 National Institute Of Biological Sciences, Beijing Méthodes de traitement de déficiences ou de cancers complexes par modulation de la biosynthèse de gro3p

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69207749T2 (de) * 1991-01-16 1996-06-13 Kirin Brewery Kälteresistente pflanzen und ihre herstellung
US6103520A (en) 1993-09-03 2000-08-15 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Plant DNA encoding glycerol-3-phosphate dehydrogenase (GPDH)
GB9510927D0 (en) * 1995-05-31 1995-07-26 Ca Nat Research Council Plant and seed oil modification
AU706899B2 (en) 1995-07-27 1999-07-01 Kirin Holdings Kabushiki Kaisha DNA strands coding for glycerol-3-phosphate acyltransferase
WO1999028480A1 (fr) 1997-12-02 1999-06-10 E.I. Du Pont De Nemours And Company Procede de production de glycerol par des organismes de recombinaison
US7579517B2 (en) 2002-05-08 2009-08-25 Basf Plant Science Gmbh Methods for increasing oil content in plants

Also Published As

Publication number Publication date
CA2385353C (fr) 2010-04-20
AU7399100A (en) 2001-04-24
EP1214432A1 (fr) 2002-06-19
WO2001021820A1 (fr) 2001-03-29
US7112724B1 (en) 2006-09-26

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