CA2379212A1 - Recherche de specificite et de sensibilite a l'hybridation d'oligonucleotides - Google Patents

Recherche de specificite et de sensibilite a l'hybridation d'oligonucleotides Download PDF

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Publication number
CA2379212A1
CA2379212A1 CA002379212A CA2379212A CA2379212A1 CA 2379212 A1 CA2379212 A1 CA 2379212A1 CA 002379212 A CA002379212 A CA 002379212A CA 2379212 A CA2379212 A CA 2379212A CA 2379212 A1 CA2379212 A1 CA 2379212A1
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CA
Canada
Prior art keywords
polynucleotide
sample
molecules
different
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002379212A
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English (en)
Inventor
Julja Burchard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rosetta Inpharmatics LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2379212A1 publication Critical patent/CA2379212A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne des procédés et matériaux convenant à l'évaluation d'une ou de plusieurs sondes différentes et à la sélection de sondes optimisées quant à leur sensibilité et leur spécificité pour une cible particulière. Selon des réalisations préférées en particulier, les procédés et compositions de l'invention conviennent à l'évaluation de sondes polynucléotides portant différentes séquences de nucléotides. Ces procédés et compositions permettent ainsi à l'utilisateur de sélectionner une sonde polynucléotide, portant une séquence nucléotide particulière notamment, optimisée quant à sa sensibilité et/ou sa spécificité pour une molécule nucléotide cible particulière. Selon des réalisations préférées en particulier, les procédés et compositions de l'invention conviennent à l'évaluation en simultané d'une pluralité de sondes, comme sur un jeu ordonné de microéchantillons. Les sondes que l'on a utilisées pour l'évaluation selon les procédés de l'invention sont sélectionnables et utilisables pour la détection de diverses molécules, et notamment de divers polynucléotides tels que les polynucléotides génomiques, tels que l'ADN génomique, et les transcriptions génomiques, telles que les séquences d'ARN médiateurs ou d'ADN complémentaire qui en sont dérivées, mais aussi degrés de filiation géniques, notamment de transcriptions spécifiques, et de différents polymorphismes de nucléotides.
CA002379212A 1999-07-16 2000-07-14 Recherche de specificite et de sensibilite a l'hybridation d'oligonucleotides Abandoned CA2379212A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US14438299P 1999-07-16 1999-07-16
US60/144,382 1999-07-16
US15456399P 1999-09-17 1999-09-17
US60/154,563 1999-09-17
PCT/US2000/019203 WO2001006013A1 (fr) 1999-07-16 2000-07-14 Recherche de specificite et de sensibilite a l'hybridation d'oligonucleotides

Publications (1)

Publication Number Publication Date
CA2379212A1 true CA2379212A1 (fr) 2001-01-25

Family

ID=26841955

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002379212A Abandoned CA2379212A1 (fr) 1999-07-16 2000-07-14 Recherche de specificite et de sensibilite a l'hybridation d'oligonucleotides

Country Status (5)

Country Link
EP (1) EP1200625A1 (fr)
JP (1) JP2003505038A (fr)
AU (1) AU6213600A (fr)
CA (1) CA2379212A1 (fr)
WO (1) WO2001006013A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7047141B2 (en) * 2001-03-22 2006-05-16 Ge Healthcare Bio-Sciences Ab Ratio-based oligonucleotide probe selection
EP1410011B1 (fr) 2001-06-18 2011-03-23 Rosetta Inpharmatics LLC Diagnostic et prevision du cancer du sein chez des patients
US7171311B2 (en) 2001-06-18 2007-01-30 Rosetta Inpharmatics Llc Methods of assigning treatment to breast cancer patients
KR100442839B1 (ko) * 2001-12-15 2004-08-02 삼성전자주식회사 최적 탐침 설계를 위한 탐침의 점수화 및 선택에 관한 방법
KR100450816B1 (ko) * 2002-03-06 2004-10-01 삼성전자주식회사 유전자형 확인용 프로브 세트 선택 방법
EP1479780A1 (fr) * 2003-05-22 2004-11-24 Institut Pasteur Nouveau procédé de conception de sondes pour saccharomyces cérévisiae minimisant l'hybridation croisée, les sondes obtenues, et leurs utilisations diagnostiques
EP1726648A4 (fr) * 2004-03-19 2008-04-09 Toyo Boseki Puce adn et procede de detection de polymorphisme d' un seul nucleotide
US20050233339A1 (en) * 2004-04-20 2005-10-20 Barrett Michael T Methods and compositions for determining the relationship between hybridization signal of aCGH probes and target genomic DNA copy number
US8105777B1 (en) 2008-02-13 2012-01-31 Nederlands Kanker Instituut Methods for diagnosis and/or prognosis of colon cancer

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6040138A (en) * 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US6110676A (en) * 1996-12-04 2000-08-29 Boston Probes, Inc. Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays

Also Published As

Publication number Publication date
EP1200625A1 (fr) 2002-05-02
WO2001006013A1 (fr) 2001-01-25
JP2003505038A (ja) 2003-02-12
AU6213600A (en) 2001-02-05

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Legal Events

Date Code Title Description
FZDE Discontinued
FZDE Discontinued

Effective date: 20040714