CA2376535A1 - Cort-pcr assay for the discrimination of endogenous reverse transcriptase activity in eukaryotic cell lines from infectious retrovirus contamination - Google Patents

Cort-pcr assay for the discrimination of endogenous reverse transcriptase activity in eukaryotic cell lines from infectious retrovirus contamination Download PDF

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Publication number
CA2376535A1
CA2376535A1 CA002376535A CA2376535A CA2376535A1 CA 2376535 A1 CA2376535 A1 CA 2376535A1 CA 002376535 A CA002376535 A CA 002376535A CA 2376535 A CA2376535 A CA 2376535A CA 2376535 A1 CA2376535 A1 CA 2376535A1
Authority
CA
Canada
Prior art keywords
reverse transcriptase
cell lines
activity
cort
discrimination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002376535A
Other languages
French (fr)
Other versions
CA2376535C (en
Inventor
Scott Goebel
Maurice Harmon
William Lapps
Francois Pelloquin
Dennis W. Trent
Jim Tartaglia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi Pasteur Inc
Original Assignee
Aventis Pasteur
Scott Goebel
Maurice Harmon
Jim Tartaglia
William Lapps
Francois Pelloquin
Dennis W. Trent
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pasteur, Scott Goebel, Maurice Harmon, Jim Tartaglia, William Lapps, Francois Pelloquin, Dennis W. Trent filed Critical Aventis Pasteur
Publication of CA2376535A1 publication Critical patent/CA2376535A1/en
Application granted granted Critical
Publication of CA2376535C publication Critical patent/CA2376535C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses

Abstract

The present invention relates to the detection of the presence of reverse transcriptase enzymatic activity in eukary-otic cell lines. Specifically, the present invention comprises a method for discriminating between endogenous reverse transcriptase activity in a substrate cell line (e.g., avian) used as substrates for the manufacture of biological products (including viral vaccines) from exogenous infectious retrovirus by using a combination of transmissibility assay (viral replication) and PBRT assay (RT-PCR).
Appropriate levels of viral amplification and number of PCR cycles are determined and employed that permit detection of exogenous infectious RT activity but not endogenous RT activity.
CA002376535A 1999-06-14 2000-06-14 Cort-pcr assay for the discrimination of endogenous reverse transcriptase activity in eukaryotic cell lines from infectious retrovirus contamination Expired - Fee Related CA2376535C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13897399P 1999-06-14 1999-06-14
US60/138,973 1999-06-14
PCT/US2000/016347 WO2000077243A2 (en) 1999-06-14 2000-06-14 CoRT-PCR ASSAY FOR THE DISCRIMINATION OF ENDOGENOUS REVERSE TRANSCRIPTASE ACTIVITY IN EUKARYOTIC CELL LINES FROM INFECTIOUS RETROVIRUS CONTAMINATION

Publications (2)

Publication Number Publication Date
CA2376535A1 true CA2376535A1 (en) 2000-12-21
CA2376535C CA2376535C (en) 2007-09-11

Family

ID=22484526

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002376535A Expired - Fee Related CA2376535C (en) 1999-06-14 2000-06-14 Cort-pcr assay for the discrimination of endogenous reverse transcriptase activity in eukaryotic cell lines from infectious retrovirus contamination

Country Status (8)

Country Link
US (2) US6465171B1 (en)
EP (1) EP1185703B1 (en)
JP (1) JP2003517813A (en)
AT (1) ATE291640T1 (en)
AU (1) AU785093B2 (en)
CA (1) CA2376535C (en)
DE (1) DE60018926T2 (en)
WO (1) WO2000077243A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7083912B2 (en) * 2002-05-31 2006-08-01 Penn State Research Foundation MAb-based Dot-ELISA method and assay kit for the detection of viruses
EP2019687B1 (en) 2006-03-29 2014-03-19 Merial Limited Vaccine against streptococci
CN103808945B (en) * 2014-03-12 2016-02-10 山东农业大学 A kind of J subgroup avian leucosis immune colloid gold antibody test test strips
WO2016106113A1 (en) 2014-12-23 2016-06-30 Ge Healthcare Uk Limited Methods and reagents for reverse-transcription polymerase chain reaction
WO2016106129A2 (en) 2014-12-23 2016-06-30 Ge Healthcare Uk Limited Methods and reagents for reverse-transcription polymerase chain reaction
WO2016106111A1 (en) 2014-12-23 2016-06-30 Ge Healthcare Uk Limited Methods and reagents for reverse-transcription polymerase chain reaction
CN109116019A (en) * 2018-07-20 2019-01-01 广西大学 The detection method of A, B, J subgroup avian leucosis virus in newcastle disease, infectiousness bronchitis bigeminy live vaccine

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3755086A (en) 1971-02-09 1973-08-28 Hoffmann La Roche Diagnostic method utilizing synthetic deoxyrilionucleotide oligomer template
EP0623176B1 (en) * 1992-05-11 2000-01-19 Jörg SCHÜPBACH Process for detecting reverse transcriptase

Also Published As

Publication number Publication date
JP2003517813A (en) 2003-06-03
AU5611500A (en) 2001-01-02
ATE291640T1 (en) 2005-04-15
EP1185703A2 (en) 2002-03-13
US20030008280A1 (en) 2003-01-09
EP1185703B1 (en) 2005-03-23
AU785093B2 (en) 2006-09-14
DE60018926D1 (en) 2005-04-28
WO2000077243A3 (en) 2001-05-10
DE60018926T2 (en) 2006-04-13
US6465171B1 (en) 2002-10-15
CA2376535C (en) 2007-09-11
WO2000077243A2 (en) 2000-12-21

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