CA2367037A1 - Syteme de sequence activatrice a mediation assuree par compose de liaison a l'adn - Google Patents
Syteme de sequence activatrice a mediation assuree par compose de liaison a l'adn Download PDFInfo
- Publication number
- CA2367037A1 CA2367037A1 CA002367037A CA2367037A CA2367037A1 CA 2367037 A1 CA2367037 A1 CA 2367037A1 CA 002367037 A CA002367037 A CA 002367037A CA 2367037 A CA2367037 A CA 2367037A CA 2367037 A1 CA2367037 A1 CA 2367037A1
- Authority
- CA
- Canada
- Prior art keywords
- binding
- sequence
- dna
- promoter
- compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4738—Cell cycle regulated proteins, e.g. cyclin, CDC, INK-CCR
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/40—Systems of functionally co-operating vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/55—Vector systems having a special element relevant for transcription from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/60—Vector systems having a special element relevant for transcription from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/75—Vector systems having a special element relevant for transcription from invertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
- C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
L'invention concerne des procédés et des compositions permettant la mise en oeuvre d'un système de séquence activatrice destiné à être utilisé dans l'expression génique à régulation. Le système comprend une construction d'acide nucléique dotée d'une séquence de réponse d'ADN pour protéine régulatrice transcriptionnelle reliée opérationnelle à un promoteur, une séquence de liaison de composé au voisinage de la séquence de réponse en question, un transgène sous le contrôle du promoteur, et un composé de liaison à l'ADN. Dans certains cas, le système de séquence activatrice comprend également une séquence d'acide nucléique codant une protéine régulatrice transcriptionnelle reliée opérationnelle à un second promoteur. L'invention concerne en outre un procédé de criblage des composés permettant de détecter l'aptitude de ces composés à fonctionner dans le système de séquence activatrice et à réguler ainsi l'expression génique.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12251399P | 1999-03-03 | 1999-03-03 | |
US60/122,513 | 1999-03-03 | ||
US15460599P | 1999-09-17 | 1999-09-17 | |
US60/154,605 | 1999-09-17 | ||
PCT/US2000/005728 WO2000052179A2 (fr) | 1999-03-03 | 2000-03-03 | Syteme de sequence activatrice a mediation assuree par compose de liaison a l'adn |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2367037A1 true CA2367037A1 (fr) | 2000-09-08 |
Family
ID=26820607
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002367037A Abandoned CA2367037A1 (fr) | 1999-03-03 | 2000-03-03 | Syteme de sequence activatrice a mediation assuree par compose de liaison a l'adn |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1165808A2 (fr) |
AU (1) | AU3513000A (fr) |
CA (1) | CA2367037A1 (fr) |
WO (1) | WO2000052179A2 (fr) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001053479A2 (fr) * | 2000-01-24 | 2001-07-26 | Sangamo Biosciences, Inc. | Sequences activatrices ii |
EP1292688A2 (fr) | 2000-06-06 | 2003-03-19 | Genelabs Technologies, Inc. | Promoteurs destines a l'expression genique regulee |
WO2002048195A2 (fr) * | 2000-10-23 | 2002-06-20 | Engeneos, Inc. | Commutateurs artificiels reagissant a un stimulus |
ES2299284B1 (es) * | 2004-11-04 | 2009-04-16 | Universidad Pablo De Olavide | Control de la expresion genica mediante el uso de un atenuador de la transcripcion. |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07509694A (ja) * | 1992-05-14 | 1995-10-26 | ベイラー・カレッジ・オブ・メディシン | 突然変異したステロイドホルモン受容体,その使用方法および遺伝子治療のための分子スイッチ |
AU684524B2 (en) * | 1993-06-14 | 1997-12-18 | Tet Systems Holding Gmbh & Co. Kg | Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters |
WO1996037609A1 (fr) * | 1995-05-26 | 1996-11-28 | Zeneca Limited | Commutateur de gene comprenant un recepteur d'ecdysone |
WO1996041865A1 (fr) * | 1995-06-07 | 1996-12-27 | Ariad Gene Therapeutics, Inc. | Regulation d'evenements biologiques fondee sur la rapamycine |
AU725399B2 (en) * | 1995-09-15 | 2000-10-12 | Baylor College Of Medicine | Steroid receptor coactivator compositions and methods of use |
US5968773A (en) * | 1997-11-14 | 1999-10-19 | Heddle; John A. | System and method for regulation of gene expression |
GB9817704D0 (en) * | 1998-08-13 | 1998-10-07 | Zeneca Ltd | Gene switch |
-
2000
- 2000-03-03 EP EP00913742A patent/EP1165808A2/fr not_active Withdrawn
- 2000-03-03 WO PCT/US2000/005728 patent/WO2000052179A2/fr not_active Application Discontinuation
- 2000-03-03 CA CA002367037A patent/CA2367037A1/fr not_active Abandoned
- 2000-03-03 AU AU35130/00A patent/AU3513000A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
AU3513000A (en) | 2000-09-21 |
EP1165808A2 (fr) | 2002-01-02 |
WO2000052179A3 (fr) | 2000-12-21 |
WO2000052179A2 (fr) | 2000-09-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Discontinued |