CA2362440C - Manipulation genetique d'isoflavonoides - Google Patents

Manipulation genetique d'isoflavonoides Download PDF

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Publication number
CA2362440C
CA2362440C CA002362440A CA2362440A CA2362440C CA 2362440 C CA2362440 C CA 2362440C CA 002362440 A CA002362440 A CA 002362440A CA 2362440 A CA2362440 A CA 2362440A CA 2362440 C CA2362440 C CA 2362440C
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Prior art keywords
isoflavonoid
leu
plant
isoflavone
gene
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Expired - Fee Related
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CA002362440A
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CA2362440A1 (fr
Inventor
Christopher L. Steele
Richard A. Dixon
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Roberts Samuels Noble Foundation Inc
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Roberts Samuels Noble Foundation Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne les gènes CYP93C du soja et de Medicago truncatula isolés, ces gènes codant pour un cytochrome P-450 capable de catalyser la migration aryle d'une flavanone pour produire un produit intermédiaire d'isoflavanone ou une isoflavone. Il est maintenant possible de modifier génétiquement des végétaux pour produire des isoflavones susceptibles d'être bénéfiques pour l'homme et d'améliorer la résistance aux maladies chez les végétaux. On peut donc produire des isoflavones dans des espèces végétales transgéniques ne contenant pas naturellement d'isoflavones, autrement dans des espèces autres que les légumineuses. Dans une variante, on peut provoquer une biosynthèse d'isoflavonoïdes, induite par une infection, dans des espèces non légumineuses, ce qui vient compléter avec avantage la défense de la phytoalexine contre les agents pathogènes microbiens, une surexpression de la voie des isoflavonoïdes dans les légumineuses permettant par ailleurs une amélioration qualitative de cette réaction de défense. Enfin, on peut transformer positivement l'efficacité de nodulation, et donc le rendement végétal, en modifiant l'importance de la production d'isoflavonoïdes dans les racines des légumineuses.
CA002362440A 1999-03-08 2000-03-08 Manipulation genetique d'isoflavonoides Expired - Fee Related CA2362440C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12326799P 1999-03-08 1999-03-08
US60/123,267 1999-03-08
PCT/US2000/005915 WO2000053771A1 (fr) 1999-03-08 2000-03-08 Manipulation génétique d'isoflavonoïdes

Publications (2)

Publication Number Publication Date
CA2362440A1 CA2362440A1 (fr) 2000-09-14
CA2362440C true CA2362440C (fr) 2009-12-08

Family

ID=22407663

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002362440A Expired - Fee Related CA2362440C (fr) 1999-03-08 2000-03-08 Manipulation genetique d'isoflavonoides

Country Status (5)

Country Link
EP (1) EP1161540A1 (fr)
AU (1) AU776161B2 (fr)
CA (1) CA2362440C (fr)
NZ (1) NZ513992A (fr)
WO (1) WO2000053771A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7098011B1 (en) 1999-01-27 2006-08-29 E. I. Du Pont De Nemours And Company Nucleic acid sequences encoding isoflavone synthase
US7208659B2 (en) 2001-05-02 2007-04-24 Conopco Inc. Process for increasing the flavonoid content of a plant and plants obtainable thereby
CN1650017A (zh) * 2002-02-28 2005-08-03 荷兰联合利华有限公司 转基因植物中大豆黄素的制备
AU2003263740A1 (en) 2002-06-14 2003-12-31 Syngenta Participations Ag Flavonol expressing domesticated tomato and method of production
EP1555870A4 (fr) * 2002-09-10 2006-11-02 Samuel Roberts Noble Found Inc Procedes et compositions permettant de generer des produits nutraceutiques contenant des flavonoides et des isoflavonoides
CN114891807B (zh) * 2022-05-30 2024-01-26 中国农业科学院北京畜牧兽医研究所 Cyp450基因在调控大豆异黄酮合成中的应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5750399A (en) * 1994-11-14 1998-05-12 The Samuel Roberts Noble Foundation, Inc. Isoflavone reductase promoter
US6121512A (en) * 1997-10-10 2000-09-19 North Carolina State University Cytochrome P-450 constructs and method of producing herbicide-resistant transgenic plants
HUP0200286A3 (en) * 1999-01-27 2003-12-29 Du Pont Nucleic acid sequences encoding isoflavone synthase

Also Published As

Publication number Publication date
AU776161B2 (en) 2004-08-26
CA2362440A1 (fr) 2000-09-14
WO2000053771A1 (fr) 2000-09-14
EP1161540A1 (fr) 2001-12-12
NZ513992A (en) 2004-03-26
AU3728700A (en) 2000-09-28

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