CA2356189A1 - Substance for obtaining highly effective tumor medications as well as a process - Google Patents
Substance for obtaining highly effective tumor medications as well as a process Download PDFInfo
- Publication number
- CA2356189A1 CA2356189A1 CA002356189A CA2356189A CA2356189A1 CA 2356189 A1 CA2356189 A1 CA 2356189A1 CA 002356189 A CA002356189 A CA 002356189A CA 2356189 A CA2356189 A CA 2356189A CA 2356189 A1 CA2356189 A1 CA 2356189A1
- Authority
- CA
- Canada
- Prior art keywords
- glycoprotein
- substances
- substance
- tumor
- bind
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000126 substance Substances 0.000 title claims abstract 25
- 206010028980 Neoplasm Diseases 0.000 title claims 7
- 238000000034 method Methods 0.000 title claims 7
- 229940079593 drug Drugs 0.000 title 1
- 239000003814 drug Substances 0.000 title 1
- 238000002483 medication Methods 0.000 title 1
- 102000003886 Glycoproteins Human genes 0.000 claims abstract 24
- 108090000288 Glycoproteins Proteins 0.000 claims abstract 24
- 239000003795 chemical substances by application Substances 0.000 claims abstract 6
- 102100025680 Complement decay-accelerating factor Human genes 0.000 claims abstract 4
- 101000856022 Homo sapiens Complement decay-accelerating factor Proteins 0.000 claims abstract 4
- 238000004519 manufacturing process Methods 0.000 claims abstract 4
- 230000000259 anti-tumor effect Effects 0.000 claims abstract 2
- 210000004881 tumor cell Anatomy 0.000 claims 6
- 230000006907 apoptotic process Effects 0.000 claims 5
- 210000004027 cell Anatomy 0.000 claims 5
- 238000002360 preparation method Methods 0.000 claims 4
- 238000003745 diagnosis Methods 0.000 claims 3
- 230000001939 inductive effect Effects 0.000 claims 3
- 239000012528 membrane Substances 0.000 claims 3
- 102000012338 Poly(ADP-ribose) Polymerases Human genes 0.000 claims 2
- 108010061844 Poly(ADP-ribose) Polymerases Proteins 0.000 claims 2
- 229920000776 Poly(Adenosine diphosphate-ribose) polymerase Polymers 0.000 claims 2
- 239000013543 active substance Substances 0.000 claims 2
- 239000000654 additive Substances 0.000 claims 2
- 208000009956 adenocarcinoma Diseases 0.000 claims 2
- 239000002671 adjuvant Substances 0.000 claims 2
- 239000002246 antineoplastic agent Substances 0.000 claims 2
- 230000026731 phosphorylation Effects 0.000 claims 2
- 238000006366 phosphorylation reaction Methods 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 230000009870 specific binding Effects 0.000 claims 2
- 239000003981 vehicle Substances 0.000 claims 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 230000027455 binding Effects 0.000 claims 1
- 230000025084 cell cycle arrest Effects 0.000 claims 1
- 238000003776 cleavage reaction Methods 0.000 claims 1
- 239000012636 effector Substances 0.000 claims 1
- 238000002372 labelling Methods 0.000 claims 1
- 230000004807 localization Effects 0.000 claims 1
- 230000001404 mediated effect Effects 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 230000007017 scission Effects 0.000 claims 1
- 238000001542 size-exclusion chromatography Methods 0.000 claims 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims 1
- 238000002560 therapeutic procedure Methods 0.000 claims 1
- 230000001052 transient effect Effects 0.000 claims 1
- 239000012634 fragment Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4747—Apoptosis related proteins
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Cell Biology (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Plant Substances (AREA)
- Steroid Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to a substance and a method for producing anti-tumour agents. This substance corresponds to a fragment of the glycoprotein CD55/DA F and can also be used for diagnostic purposes.
Claims (29)
1. Glycoprotein that comprises at least one section of the amino acid primary structure of CD55 and a tumor-specific glycostructure.
2. Glycoprotein according to claim 1, characterized in that the glycostructure reacts with monoclonal antibody SC-1.
3. Glycoprotein according to claim 1 or 2, wherein in SDS-polyacrylamide-gel electrophoresis, it exhibits an apparent molecular weight of 82 kD.
4. Process for obtaining a glycoprotein according to one of claims 1 to 3, wherein membrane preparations are produced from cells of the human adenocarcinoma cell line 23132, and the glycoprotein is obtained therefrom by size-exclusion and/or anion-exchange chromatography.
5. Use of a glycoprotein according to one of claims 1 to 3 in a test process in which the ability of a substance to bind to the glycoprotein is determined.
6. Use according to claim 5, wherein the ability to bind to the glycostructure is determined.
7. Use according to claim 5 or 6, wherein the ability of the tested substance to induce apoptosis, especially in tumor cells, is determined.
8. Use according to one of claims 5 to 7, wherein the ability of the tested substance to induce a phosphorylation cascade that is mediated by glycoprotein CD55 is determined.
9. Use according to claims 5 to 8, wherein the glycoprotein is used in isolated form, as a cell extract, especially as a membrane preparation or in the form of complete cells, especially of human adenocarcinoma cell line 23132.
10. Use according to one of claims 5 to 9 for identifying substances that bind specifically to tumor cells.
11. Use according to claim 10 for identifying agents for tumor diagnosis and/or tumor therapy.
12. Use according to one of claims 5 to 11, wherein the pharmacologically compatible substances are tested.
13. Use according to claim 12, wherein the tested substances are selected from peptides, peptide mimetic agents, antibodies, antibody fragments and antibody derivatives.
14. Use of substances that bind specifically to a glycoprotein according to one of claims 1 to 3, with the exception of the monoclonal antibody SC-1, for the production of agents that induce apoptosis.
15. Use of substances that bind specifically to a glycoprotein according to one of claims 1 to 3, with the exception of monoclonal antibody SC-1, for the production of anti-tumor agents.
16. Use of substances that bind specifically to a glycoprotein according to one of claims 1 to 3, with the exception of monoclonal antibody SC-1, for the production of agents for tumor diagnosis.
17. Process for the preparation of the agents that induce apoptosis, wherein a potentially active substance is tested on its ability for specific binding to a glycoprotein according to one of claims 1 to 3 and in the case of a positive test result, the substance is converted into a form for dispensing that is suitable for pharmaceutical applications optionally together with commonly used adjuvants, additives and vehicles.
18. Process for the preparation of anti-tumor agents, wherein a potentially active substance is tested on its ability for specific binding to a glycoprotein according to one of claims 1 to 3 and in the case of a positive test result, the substance is converted into a form for dispensing that is suitable for pharmaceutical applications optionally together with commonly used adjuvants, additives and vehicles.
19. Process for combatting tumors, wherein an anti-tumor-action quantity of a substance that can bind specifically to a glycoprotein according to one of claims 1 to 3, with the exception of monoclonal antibody SC-1, is administered to a patient.
20. Process for diagnosis of tumors, wherein a sample that is to be tested or a patient who is to be tested is brought into contact with a substance that can bind specifically to a glycoprotein according to one of claims 1 to 3, and the presence, the localization and/or the quantity of the glycoprotein in the sample or in the patient is detected.
21. Use of substances that specifically bind a glycoprotein according to one of claims 1 to 3 to trigger a phosphorylation cascade in tumor cells.
22. Use of substances that bind specifically to a glycoprotein according to one of claims 1 to 3 for transient increase of the CD55/DAF presentation in membranes of tumor cells.
23. Use of substances that specifically bind a glycoprotein according to one of claims 1 to 3 for inducing apoptotic processes that do not comprise any cleavage of poly(ADP-ribose)-polymerase (PARP).
24. Use of substances that specifically bind a glycoprotein according to one of claims 1 to 3 for inducing a cell cycle arrest in tumor cells.
25. Use of substances that bind specifically to a glycoprotein according to one of claims 1 to 3 for inducing apoptosis in dormant tumor cells.
26. Use according to one of claims 21 to 25, wherein the specifically bindable substance comprises antibody SC-1.
27. Use according to one of claims 21 to 26, wherein the substances are used in the form of conjugates with labeling or effector groups.
28. Use according to one of claims 21 to 27, wherein the substances have multiple binding sites for a glycoprotein according to one of claims 1 to 3.
29. Use according to claim 28, wherein the specifically bindable substances are cross-linked.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19859248 | 1998-12-22 | ||
| DE19859248.5 | 1998-12-22 | ||
| DE19909771.2 | 1999-03-05 | ||
| DE19909771A DE19909771A1 (en) | 1998-12-22 | 1999-03-05 | Substance for the production of highly effective tumor drugs and methods |
| PCT/EP1999/010329 WO2000037489A2 (en) | 1998-12-22 | 1999-12-22 | Fragment of the gylcoprotein cd55/daf for producing highly effective anti-tumour medicaments and method for using the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2356189A1 true CA2356189A1 (en) | 2000-06-29 |
| CA2356189C CA2356189C (en) | 2010-08-24 |
Family
ID=26050926
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2356189A Expired - Fee Related CA2356189C (en) | 1998-12-22 | 1999-12-22 | Substance for obtaining highly effective tumor medications as well as a process |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP1141019B1 (en) |
| AT (1) | ATE264873T1 (en) |
| AU (2) | AU772579B2 (en) |
| CA (1) | CA2356189C (en) |
| DK (1) | DK1141019T3 (en) |
| ES (1) | ES2219108T3 (en) |
| PT (1) | PT1141019E (en) |
| WO (1) | WO2000037489A2 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2002325164B2 (en) | 2001-07-24 | 2008-12-04 | Debiovision Inc. | Receptor, the use thereof, and mouse antibodies |
| WO2004005351A2 (en) | 2002-07-04 | 2004-01-15 | Oncomab Gmbh | Neoplasm specific antibodies and uses thereof |
| GB0227644D0 (en) * | 2002-11-27 | 2003-01-08 | Cancer Rec Tech Ltd | Specific binding members and uses thereof |
| DE10311248A1 (en) | 2003-03-14 | 2004-09-30 | Müller-Hermelink, Hans Konrad, Prof. Dr. | Human monoclonal antibody |
| DE10353175A1 (en) | 2003-11-14 | 2005-06-16 | Müller-Hermelink, Hans Konrad, Prof. Dr. | Human monoclonal antibody with fatliquoring effect |
| EP1531162A1 (en) | 2003-11-14 | 2005-05-18 | Heinz Vollmers | Adenocarcinoma specific antibody SAM-6, and uses thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5374548A (en) * | 1986-05-02 | 1994-12-20 | Genentech, Inc. | Methods and compositions for the attachment of proteins to liposomes using a glycophospholipid anchor |
| GB9804065D0 (en) * | 1998-02-26 | 1998-04-22 | Cancer Res Campaign Tech | Tumour associated antigen 791Tgp72 |
-
1999
- 1999-12-22 ES ES99969227T patent/ES2219108T3/en not_active Expired - Lifetime
- 1999-12-22 EP EP99969227A patent/EP1141019B1/en not_active Expired - Lifetime
- 1999-12-22 WO PCT/EP1999/010329 patent/WO2000037489A2/en active IP Right Grant
- 1999-12-22 DK DK99969227T patent/DK1141019T3/en active
- 1999-12-22 CA CA2356189A patent/CA2356189C/en not_active Expired - Fee Related
- 1999-12-22 AU AU27955/00A patent/AU772579B2/en not_active Ceased
- 1999-12-22 AT AT99969227T patent/ATE264873T1/en active
- 1999-12-22 PT PT99969227T patent/PT1141019E/en unknown
-
2004
- 2004-07-29 AU AU2004203479A patent/AU2004203479A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1141019B1 (en) | 2004-04-21 |
| DK1141019T3 (en) | 2004-08-09 |
| AU772579B2 (en) | 2004-04-29 |
| ATE264873T1 (en) | 2004-05-15 |
| AU2795500A (en) | 2000-07-12 |
| AU2004203479A1 (en) | 2004-08-26 |
| PT1141019E (en) | 2004-09-30 |
| CA2356189C (en) | 2010-08-24 |
| EP1141019A2 (en) | 2001-10-10 |
| WO2000037489A3 (en) | 2000-11-09 |
| ES2219108T3 (en) | 2004-11-16 |
| WO2000037489A2 (en) | 2000-06-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20161222 |