CA2355579A1 - Enzymes subtilases des sous-groupes i-s1 et i-s2 ayant un residu d'acide amine additionnel dans une region boucle de site actif - Google Patents

Enzymes subtilases des sous-groupes i-s1 et i-s2 ayant un residu d'acide amine additionnel dans une region boucle de site actif Download PDF

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Publication number
CA2355579A1
CA2355579A1 CA002355579A CA2355579A CA2355579A1 CA 2355579 A1 CA2355579 A1 CA 2355579A1 CA 002355579 A CA002355579 A CA 002355579A CA 2355579 A CA2355579 A CA 2355579A CA 2355579 A1 CA2355579 A1 CA 2355579A1
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CA
Canada
Prior art keywords
subtilase
amino acid
group
variant
acid residue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002355579A
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English (en)
Other versions
CA2355579C (fr
Inventor
Kim Andersen Vilbour
Frank Mikkelsen
Peter Hansen Kamp
Carsten Andersen
Mads Norregaard-Madsen
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Novozymes AS
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Individual
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Publication of CA2355579A1 publication Critical patent/CA2355579A1/fr
Application granted granted Critical
Publication of CA2355579C publication Critical patent/CA2355579C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Cette invention se rapporte à des enzymes subtilases des sous-groupes I-S1 et I-S2 comportant un résidu d'acide aminé additionnel dans la position 100 de la région boucle de site actif (b) entre la position 95 et 103. Des variants de ces substilases possèdent des performances de lavage améliorées dans un détergent par rapport à leur enzyme parente.
CA2355579A 1998-12-18 1999-12-20 Enzymes subtilases des sous-groupes i-s1 et i-s2 ayant un residu d'acide amine additionnel dans une region boucle de site actif Expired - Fee Related CA2355579C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA199801673 1998-12-18
DKPA199801673 1998-12-18
PCT/DK1999/000715 WO2000037624A1 (fr) 1998-12-18 1999-12-20 Enzymes subtilases des sous-groupes i-s1 et i-s2 ayant un residu d'acide amine additionnel dans une region boucle de site actif

Publications (2)

Publication Number Publication Date
CA2355579A1 true CA2355579A1 (fr) 2000-06-29
CA2355579C CA2355579C (fr) 2011-11-22

Family

ID=8107093

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2355579A Expired - Fee Related CA2355579C (fr) 1998-12-18 1999-12-20 Enzymes subtilases des sous-groupes i-s1 et i-s2 ayant un residu d'acide amine additionnel dans une region boucle de site actif

Country Status (7)

Country Link
JP (1) JP4647788B2 (fr)
KR (1) KR100660799B1 (fr)
CN (1) CN1302106C (fr)
AU (1) AU773327B2 (fr)
BR (1) BRPI9916350B1 (fr)
CA (1) CA2355579C (fr)
WO (1) WO2000037624A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023225459A2 (fr) 2022-05-14 2023-11-23 Novozymes A/S Compositions et procédés de prévention, de traitement, de suppression et/ou d'élimination d'infestations et d'infections phytopathogènes

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04500385A (ja) * 1989-06-26 1992-01-23 ユニリーバー・ナームローゼ・ベンノートシヤープ 酵素洗剤組成物
DK316989D0 (da) * 1989-06-26 1989-06-26 Novo Nordisk As Enzymer
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
WO1996034935A2 (fr) * 1995-05-05 1996-11-07 Unilever N.V. Variantes de subtilisine
DK0985042T3 (da) * 1997-06-04 2007-01-15 Genencor Int Proteaseenzymer til vanskelig rengöring og/eller plet- og filmreduktion samt præparater, der inkorporerer disse
BR9814236A (pt) * 1997-11-21 2000-10-03 Novo Nordisk As Enzima subtilase, sua variante, sequência de dna isolada, vetor de expressão, célula microbiana hospedeira, processo para produzir uma subtilase ou uma variante de subtilase, composição, e, uso de uma subtilase ou de uma variante de subtilase.

Also Published As

Publication number Publication date
KR20010093172A (ko) 2001-10-27
AU773327B2 (en) 2004-05-20
BR9916350A (pt) 2001-09-18
KR100660799B1 (ko) 2006-12-26
JP4647788B2 (ja) 2011-03-09
JP2002533077A (ja) 2002-10-08
AU1772900A (en) 2000-07-12
CN1333825A (zh) 2002-01-30
BRPI9916350B1 (pt) 2016-04-12
WO2000037624A1 (fr) 2000-06-29
CN1302106C (zh) 2007-02-28
CA2355579C (fr) 2011-11-22

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Effective date: 20171220