CA2350871A1 - Portable apparatus for detecting the transmittance and luminescence of a sample - Google Patents
Portable apparatus for detecting the transmittance and luminescence of a sample Download PDFInfo
- Publication number
- CA2350871A1 CA2350871A1 CA 2350871 CA2350871A CA2350871A1 CA 2350871 A1 CA2350871 A1 CA 2350871A1 CA 2350871 CA2350871 CA 2350871 CA 2350871 A CA2350871 A CA 2350871A CA 2350871 A1 CA2350871 A1 CA 2350871A1
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- Prior art keywords
- sample
- transmittance
- detectors
- fluorescence
- emitters
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
- G01N21/5907—Densitometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6491—Measuring fluorescence and transmission; Correcting inner filter effect
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/02—Mechanical
- G01N2201/022—Casings
- G01N2201/0221—Portable; cableless; compact; hand-held
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- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
An apparatus for simultaneously detecting the transmittance and the luminescence (fluorescence and phosphorescence) in a sample in dish is described herein. The apparatus comprises two light emitters in the form of DEL, transmittance and fluorescence detectors in the form of photodiodes, and a controller for determining the concentration of a predetermined biological agent in the biological sample using signals coming from the detectors. The body is so configured and sized and the emitters and detectors are so mounted thereto to provide a portable and compact measurement apparatus that does not require a complex optical assembly.
Description
TITLE OF THE INVENTION
PORTABLE APPARATUS FOR DETECTING THE
TRANSMITTANCE AND LUMINESCENCE OF A SAMPLE
FIELD OF THE INVENTION
The present invention relates to apparatuses for detecting transmittance and luminescence of samples. More specifically, the present 10 invention is concerned with such an apparatus that is portable.
BACKGROUND OF THE INVENTION
The quantization of a particular material is useful in diverse field of technology such as biology, biochemistry, chemistry and material science. Two of the more well known techniques for material quantization are photometry and fluorometry.
In photometry, a beam of light is directed toward a sample of the material to be quantified, and the amount of light absorbed by the material as the light passes through it is measured to quantify the material.
Fluorimetry, in contrast to photometry is based on the phenomena whereby a material emits the light of a characteristic 25 wavelength when it is properly excited. A fluorometer must be able to clearly differentiate the light which is emitted as fluorescence by the sample material from the light which is used to excite the material into its fluorescence. Photometers and fluorometers do have some commonalities.
An example of an apparatus for testing biological samples is described in the United States Patent No. 5,925,884, issued on July 20, 1999 and naming Robinson ef al. as the inventors.
A first drawback of such apparatuses is that they are relatively bulky. Moreover, their overall design and, more specifically, their optical assemblies are often complex and breakable. These two drawbacks prevent such apparatuses from being readily portable.
BRIEF DESCRIPTION OF THE DRAWINGS
In the appended drawings:
Figure 1 is a perspective view of a portable apparatus for detecting the fluorescence and the transmittance of a biological sample, according to an embodiment of the present invention, illustrated with a 20 sample dish;
Figure 2 is an exploded view of the apparatus of Figure 1;
Figure 3 is a front elevational view of one of the two symmetrical portions of the apparatus of Figure 1; and Figure 4 is a sectional view taken along line 4-4 of Figure 1.
PORTABLE APPARATUS FOR DETECTING THE
TRANSMITTANCE AND LUMINESCENCE OF A SAMPLE
FIELD OF THE INVENTION
The present invention relates to apparatuses for detecting transmittance and luminescence of samples. More specifically, the present 10 invention is concerned with such an apparatus that is portable.
BACKGROUND OF THE INVENTION
The quantization of a particular material is useful in diverse field of technology such as biology, biochemistry, chemistry and material science. Two of the more well known techniques for material quantization are photometry and fluorometry.
In photometry, a beam of light is directed toward a sample of the material to be quantified, and the amount of light absorbed by the material as the light passes through it is measured to quantify the material.
Fluorimetry, in contrast to photometry is based on the phenomena whereby a material emits the light of a characteristic 25 wavelength when it is properly excited. A fluorometer must be able to clearly differentiate the light which is emitted as fluorescence by the sample material from the light which is used to excite the material into its fluorescence. Photometers and fluorometers do have some commonalities.
An example of an apparatus for testing biological samples is described in the United States Patent No. 5,925,884, issued on July 20, 1999 and naming Robinson ef al. as the inventors.
A first drawback of such apparatuses is that they are relatively bulky. Moreover, their overall design and, more specifically, their optical assemblies are often complex and breakable. These two drawbacks prevent such apparatuses from being readily portable.
BRIEF DESCRIPTION OF THE DRAWINGS
In the appended drawings:
Figure 1 is a perspective view of a portable apparatus for detecting the fluorescence and the transmittance of a biological sample, according to an embodiment of the present invention, illustrated with a 20 sample dish;
Figure 2 is an exploded view of the apparatus of Figure 1;
Figure 3 is a front elevational view of one of the two symmetrical portions of the apparatus of Figure 1; and Figure 4 is a sectional view taken along line 4-4 of Figure 1.
DESCRIPTION OF THE PREFERRED EMBODIMENT
Referring to Figures 1 to 4 of the appended drawings, a portable apparatus 8 for detecting the fluorescence and the transmittance of a sample contained in a sample dish 12, according to a preferred embodiment of the present invention, will be described.
The apparatus comprises a body 10, two light emitters in the form of two LEDs (Light Emitting Diodes) 14 and 16, two fluorescence detectors 18 (see Figure 4) and a transmittance detector 20. The device 8 is to be connected to a controller (not shown), an input means (not shown), and a display device (not shown).
The body 10 includes a central bore 22 for receiving a sample dish 12 in a snuggly manner. A portion of the bore 22, near its distal end 24, has an H-shaped cross-section. The rest of the bore 22 has a rectangular cross-section.
The central bore 22 allows to receive either a sample dish 12 having a portion with an H-shaped cross-section (as illustrated in Figures 2 to 4), or a sample dish having a continuous rectangular cross-section.
It is to be noted that the shape and size of the bore 22 may be modified to accommodate sample dishes having other geometry, without departing from the spirit and nature of the present invention.
Referring to Figures 1 to 4 of the appended drawings, a portable apparatus 8 for detecting the fluorescence and the transmittance of a sample contained in a sample dish 12, according to a preferred embodiment of the present invention, will be described.
The apparatus comprises a body 10, two light emitters in the form of two LEDs (Light Emitting Diodes) 14 and 16, two fluorescence detectors 18 (see Figure 4) and a transmittance detector 20. The device 8 is to be connected to a controller (not shown), an input means (not shown), and a display device (not shown).
The body 10 includes a central bore 22 for receiving a sample dish 12 in a snuggly manner. A portion of the bore 22, near its distal end 24, has an H-shaped cross-section. The rest of the bore 22 has a rectangular cross-section.
The central bore 22 allows to receive either a sample dish 12 having a portion with an H-shaped cross-section (as illustrated in Figures 2 to 4), or a sample dish having a continuous rectangular cross-section.
It is to be noted that the shape and size of the bore 22 may be modified to accommodate sample dishes having other geometry, without departing from the spirit and nature of the present invention.
The body 10 further includes two recesses 26 and 28 configured to receive the two LEDs 14 and 16. Each of the two recesses 26 and 28 has a section advantageously complementary to LEDs 14 and 16 respectively. This allows to secure the LEDs 14 and 16 in the recesses 26 and 28 without requiring any securing means.
The two recesses 26 and 28 are so advantageously positioned as to orient the two LEDs 14 and 16 in a direction pointing towards a virtual point 30 (see Figures 3-4) located in the middle of the bore, facing the two emitters 14 and 16.
Each of the two recesses 26 and 28 terminates in a small passage 32 that provides a window for the light to be emitted by the two LEDs towards the virtual point 30.
The body 10 also comprises two lateral detector receptacles 34 in the form of two facing recesses in the body 10, and a central detector receptacle 36, also in the form of a recess in the body 10.
The two lateral detector receptacles 34 and the central receptacles 36 terminate in small respective passages 38 and 40 that allow the detectors 18 and 20 to collect photons from the sample in the sample dish 12 along a preferred axis intersecting the virtual point 30.
Moreover, the receptacles 34 and 36 are also so positioned to advantageously yield the same distance from the detectors 18 and 20 to the virtual point 30.
The body 10 is preferably made of a light material such as DeIrinT"". Obviously, other materials, such as aluminium may also be 5 used.
The body 10 advantageously comprises two symmetrical portions 42 and 42' that are removably secured together using screws (not shown). For that purpose, the portions 42-42' include three opposite apertures 44 for receiving the screws. Alternatively, other securing means such as cement may also be used to secure the two portions 42-42' together.
The fact that the body 10 comprises two parts is advantageous, since it allows opening of the body 10 for easy access for maintenance to the emitters 14 and16 and to the detectors 18 and 20. It also allows for easy assembly of the apparatus 8.
Optionally, the apparatus 8 may include a cover (not shown) to protect the emitters 14 and 16 and the detectors 18 and 20 during transportation of the apparatus 8. For that purpose, the body 10 advantageously includes two shoulders 46 on which a box-like cover (not shown) may rest.
The two LEDs 14 and 16 are so advantageously chosen as to provide incident light having a wavelength that allows to excite a sample containing natural pigments or doped with a dye such as rhodamine, fluorescein and Texas RedT"" .
According to a preferred embodiment of the present invention, the DELs 14 and 16 emit between 325 nm and 800 nm lights.
For example, the following DELs may be used:
~ No. LNG992CFBW by Panasonic (AID 30 nm, 1500 mcd); and ~ No. E903 by GiIwayT'"' (~I~ 25 nm, 4000 mcd).
As will be explained below, input means, preferably in the form of selector switches, allows to select one of the two emitters 14-16, or no emitter (for luminescence measurement).
Obviously, other light emitters may also be used depending on the application.
In order to allow sufficient light onto the sample, the body 10 has been configured so as to allow the emitters 14 and 16 to be positioned very closely to the sample when the sample dish 12 is correctly positioned.
The controller, to which the emitters 14 and 16 are advantageously connected, is also configured so as to provide sufficient input current to the emitters 14 and 16 depending on the desired sensitivity.
The two fluorescence detectors 18 are advantageously in the form of photodiodes, such as Burr-Brown's No. OPT21. This particular diode has the following specifications:
~ Sensitivity of 0.45 A/W (400-1100 nm);
The two recesses 26 and 28 are so advantageously positioned as to orient the two LEDs 14 and 16 in a direction pointing towards a virtual point 30 (see Figures 3-4) located in the middle of the bore, facing the two emitters 14 and 16.
Each of the two recesses 26 and 28 terminates in a small passage 32 that provides a window for the light to be emitted by the two LEDs towards the virtual point 30.
The body 10 also comprises two lateral detector receptacles 34 in the form of two facing recesses in the body 10, and a central detector receptacle 36, also in the form of a recess in the body 10.
The two lateral detector receptacles 34 and the central receptacles 36 terminate in small respective passages 38 and 40 that allow the detectors 18 and 20 to collect photons from the sample in the sample dish 12 along a preferred axis intersecting the virtual point 30.
Moreover, the receptacles 34 and 36 are also so positioned to advantageously yield the same distance from the detectors 18 and 20 to the virtual point 30.
The body 10 is preferably made of a light material such as DeIrinT"". Obviously, other materials, such as aluminium may also be 5 used.
The body 10 advantageously comprises two symmetrical portions 42 and 42' that are removably secured together using screws (not shown). For that purpose, the portions 42-42' include three opposite apertures 44 for receiving the screws. Alternatively, other securing means such as cement may also be used to secure the two portions 42-42' together.
The fact that the body 10 comprises two parts is advantageous, since it allows opening of the body 10 for easy access for maintenance to the emitters 14 and16 and to the detectors 18 and 20. It also allows for easy assembly of the apparatus 8.
Optionally, the apparatus 8 may include a cover (not shown) to protect the emitters 14 and 16 and the detectors 18 and 20 during transportation of the apparatus 8. For that purpose, the body 10 advantageously includes two shoulders 46 on which a box-like cover (not shown) may rest.
The two LEDs 14 and 16 are so advantageously chosen as to provide incident light having a wavelength that allows to excite a sample containing natural pigments or doped with a dye such as rhodamine, fluorescein and Texas RedT"" .
According to a preferred embodiment of the present invention, the DELs 14 and 16 emit between 325 nm and 800 nm lights.
For example, the following DELs may be used:
~ No. LNG992CFBW by Panasonic (AID 30 nm, 1500 mcd); and ~ No. E903 by GiIwayT'"' (~I~ 25 nm, 4000 mcd).
As will be explained below, input means, preferably in the form of selector switches, allows to select one of the two emitters 14-16, or no emitter (for luminescence measurement).
Obviously, other light emitters may also be used depending on the application.
In order to allow sufficient light onto the sample, the body 10 has been configured so as to allow the emitters 14 and 16 to be positioned very closely to the sample when the sample dish 12 is correctly positioned.
The controller, to which the emitters 14 and 16 are advantageously connected, is also configured so as to provide sufficient input current to the emitters 14 and 16 depending on the desired sensitivity.
The two fluorescence detectors 18 are advantageously in the form of photodiodes, such as Burr-Brown's No. OPT21. This particular diode has the following specifications:
~ Sensitivity of 0.45 A/W (400-1100 nm);
~ Detection area of 5,2 mm2; and ~ Conversion rate of 2x108 V/A.
Such a detector has a sensitivity of 5.5x10' photons/s/mm2 with a saturation of 1.1x10'°photons/s/mm2for a wavelength of 520 nm.
The use of two fluorescence detectors advantageously allows to increase the sensitivity by a factor of 2. Indeed, according to a preferred embodiment of the present invention, the controller includes an adder function that allows average readings from the two detectors 18. For that purpose, the two fluorescence detectors 18 are positioned at an equal distance from the emitters 14 and 16 and also from the virtual point 30.
Alternatively, a single fluorescence detector may be used.
The two fluorescence detectors 18 are advantageously positioned relative to the emitters 14 and 16 so as to maximize the fluorescence light input to the sample, while rejecting the excitation signal, without requiring the use of a colored filter or a monochromator. This is advantageous since it allows to simplify the apparatus and also minimize its size.
The transmittance detector 20 is advantageously in the form of a photodiode. Again, a Burr-Brown's No. OPT21 is used with the same specifications as listed above but with a conversion rate of 5x105 V/A.
Such a detector has a sensitivity of 2.2x10'° photons/s/mm2 with a saturation of 4.5x10'2photons/s/mm2for a wavelength of 520 nm.
Such a detector has a sensitivity of 5.5x10' photons/s/mm2 with a saturation of 1.1x10'°photons/s/mm2for a wavelength of 520 nm.
The use of two fluorescence detectors advantageously allows to increase the sensitivity by a factor of 2. Indeed, according to a preferred embodiment of the present invention, the controller includes an adder function that allows average readings from the two detectors 18. For that purpose, the two fluorescence detectors 18 are positioned at an equal distance from the emitters 14 and 16 and also from the virtual point 30.
Alternatively, a single fluorescence detector may be used.
The two fluorescence detectors 18 are advantageously positioned relative to the emitters 14 and 16 so as to maximize the fluorescence light input to the sample, while rejecting the excitation signal, without requiring the use of a colored filter or a monochromator. This is advantageous since it allows to simplify the apparatus and also minimize its size.
The transmittance detector 20 is advantageously in the form of a photodiode. Again, a Burr-Brown's No. OPT21 is used with the same specifications as listed above but with a conversion rate of 5x105 V/A.
Such a detector has a sensitivity of 2.2x10'° photons/s/mm2 with a saturation of 4.5x10'2photons/s/mm2for a wavelength of 520 nm.
Alternatively, other transmittance detectors may also be used without departing from the spirit and nature of the present invention.
The transmittance detector 20 is advantageously positioned at 180 degrees from the incident light coming from the emitters 14 or 16.
It is advantageous to use sample dishes 12 that minimize the reflection of light onto their wall, such as those made of quartz, glass and polystyrene. Also, it has been found that the use of dishes made of methacrylate allows minimization of the reflection of aqueous samples.
Dishes made of quartz, glass and polystyrene also allow minimization of the reflection.
The controller (not shown) may be in the form of a programmed chip or electronic circuits. A conventional personal computer, to which the detectors 18 and 20 and the emitters 14 and 16 are to be connected, may also be used to control the operation of the apparatus 8.
The controller is so programmed to activate the emitters 14 and 16 and to selectively collect signals from the detectors 18 and 20.
The emitters 14 and 16 and the detectors 18 and 20 are connected to the controller via cables or other connecting means.
The controller is further configured to allow detection of inhomogeneities in the sample. Indeed, the controller is configured to calculate the difference between the signals coming from the two fluorescence detectors 18, to use this difference to search for inhomogeneities in a sample, and to trigger an alarm if such an inhomogeneity is detected. Alternatively, the dish containing the sample may be rotated if there is only one fluorescence detector. A further step could the characterization of a detected inhomogeneity.
The controller is also further configured to detect saturation in the signals coming from one of the detectors 18 and 20, and to trigger an alarm accordingly.
The display device (not shown) may take many forms including: a dot matrix LED display, a digit display, a liquid crystal display, or a computer monitor. The display device allows to visualize the measurement of any one of detectors 18 and 20, or of a value computed by the controller using one of the measurements. The selection is made by the user via the input means (not shown).
The apparatus 8 further comprises a portable electrical source (not shown), advantageously in the form of rechargeable batteries.
Alternatively or additionally, the controller may be provided with a power adapter allowing to connect the apparatus 8 to a power network.
In the case where the apparatus 8 includes a portable electrical source, the controller may advantageously be so configured with a timer function that would automatically deactivate the apparatus 8 after predetermined inactivity period. The controller can also be equipped with a monitor to allow detecting power failures or power variations.
An apparatus, according to the present invention, allows to measure luminescence, transmittance and fluorescence of a biological 5 sample.
The controller is further configured to use the measured luminescence, transmittance and/or fluorescence and known algorithms to determine an approximate account of the concentration of a 10 predetermined biological agent in a biological sample.
The sensitivity of the apparatus 8 is, for example, in the range of 5 ng/ml for rhodamine 6G. This sensitivity is obtained through a 900 Hz modulation, band-pass filtering (54 dB/decade) and demodulation via an RMS function. The controller is configured to provide the above-described mode of operation.
The sensitivity may be increased by using avalanche photodiodes as detectors 16 or 18.
It is believed to be within the reach of a person skilled in the art to modify the configuration and geometry of the body 10 so as to allow for its utilization with dishes having other geometry.
Although the present invention has been described hereinabove by way of preferred embodiments thereof, it can be modified without departing from the spirit and nature of the subject invention, as
The transmittance detector 20 is advantageously positioned at 180 degrees from the incident light coming from the emitters 14 or 16.
It is advantageous to use sample dishes 12 that minimize the reflection of light onto their wall, such as those made of quartz, glass and polystyrene. Also, it has been found that the use of dishes made of methacrylate allows minimization of the reflection of aqueous samples.
Dishes made of quartz, glass and polystyrene also allow minimization of the reflection.
The controller (not shown) may be in the form of a programmed chip or electronic circuits. A conventional personal computer, to which the detectors 18 and 20 and the emitters 14 and 16 are to be connected, may also be used to control the operation of the apparatus 8.
The controller is so programmed to activate the emitters 14 and 16 and to selectively collect signals from the detectors 18 and 20.
The emitters 14 and 16 and the detectors 18 and 20 are connected to the controller via cables or other connecting means.
The controller is further configured to allow detection of inhomogeneities in the sample. Indeed, the controller is configured to calculate the difference between the signals coming from the two fluorescence detectors 18, to use this difference to search for inhomogeneities in a sample, and to trigger an alarm if such an inhomogeneity is detected. Alternatively, the dish containing the sample may be rotated if there is only one fluorescence detector. A further step could the characterization of a detected inhomogeneity.
The controller is also further configured to detect saturation in the signals coming from one of the detectors 18 and 20, and to trigger an alarm accordingly.
The display device (not shown) may take many forms including: a dot matrix LED display, a digit display, a liquid crystal display, or a computer monitor. The display device allows to visualize the measurement of any one of detectors 18 and 20, or of a value computed by the controller using one of the measurements. The selection is made by the user via the input means (not shown).
The apparatus 8 further comprises a portable electrical source (not shown), advantageously in the form of rechargeable batteries.
Alternatively or additionally, the controller may be provided with a power adapter allowing to connect the apparatus 8 to a power network.
In the case where the apparatus 8 includes a portable electrical source, the controller may advantageously be so configured with a timer function that would automatically deactivate the apparatus 8 after predetermined inactivity period. The controller can also be equipped with a monitor to allow detecting power failures or power variations.
An apparatus, according to the present invention, allows to measure luminescence, transmittance and fluorescence of a biological 5 sample.
The controller is further configured to use the measured luminescence, transmittance and/or fluorescence and known algorithms to determine an approximate account of the concentration of a 10 predetermined biological agent in a biological sample.
The sensitivity of the apparatus 8 is, for example, in the range of 5 ng/ml for rhodamine 6G. This sensitivity is obtained through a 900 Hz modulation, band-pass filtering (54 dB/decade) and demodulation via an RMS function. The controller is configured to provide the above-described mode of operation.
The sensitivity may be increased by using avalanche photodiodes as detectors 16 or 18.
It is believed to be within the reach of a person skilled in the art to modify the configuration and geometry of the body 10 so as to allow for its utilization with dishes having other geometry.
Although the present invention has been described hereinabove by way of preferred embodiments thereof, it can be modified without departing from the spirit and nature of the subject invention, as
Claims (2)
1. A portable apparatus for simultaneously detecting the fluorescence and the transmittance of a sample comprising:
a body for receiving the sample;
at least one light emitter mounted to said body; said at least one light emitter being configured so as to induce fluorescence to said sample;
at least one fluorescence detector mounted to said body;
at least one transmittance detector mounted to said body;
and a display device.
a body for receiving the sample;
at least one light emitter mounted to said body; said at least one light emitter being configured so as to induce fluorescence to said sample;
at least one fluorescence detector mounted to said body;
at least one transmittance detector mounted to said body;
and a display device.
2. A portable apparatus as recited in claim 1, further comprising a controller coupled to said at least one light emitter, to said at least one fluorescence detector and to said at least one transmittance detector for receiving signals from said detectors, for computing the concentration of a biological agent in a biological sample using at least one of said signals, and for selectively displaying on said display device at least one of said signals and said concentrations.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA 2350871 CA2350871A1 (en) | 2001-06-15 | 2001-06-15 | Portable apparatus for detecting the transmittance and luminescence of a sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA 2350871 CA2350871A1 (en) | 2001-06-15 | 2001-06-15 | Portable apparatus for detecting the transmittance and luminescence of a sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2350871A1 true CA2350871A1 (en) | 2002-12-15 |
Family
ID=4169302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2350871 Abandoned CA2350871A1 (en) | 2001-06-15 | 2001-06-15 | Portable apparatus for detecting the transmittance and luminescence of a sample |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2350871A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2244296A1 (en) * | 2003-10-03 | 2005-12-01 | Signe, S.A. | Phosphorus measurement system, has reading unit i.e. interface of data acquisition and control, and personal computer, which executes procedure for generation, acquisition and analysis of phosphorus on sample |
| WO2008101732A1 (en) * | 2007-02-23 | 2008-08-28 | Ese Embedded System Engineering Gmbh | Optical measuring instrument |
| EP3974811A1 (en) * | 2020-09-29 | 2022-03-30 | ProBiomics GmbH | Determination of parameters in biological fluids using fluorescence and absorption |
-
2001
- 2001-06-15 CA CA 2350871 patent/CA2350871A1/en not_active Abandoned
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2244296A1 (en) * | 2003-10-03 | 2005-12-01 | Signe, S.A. | Phosphorus measurement system, has reading unit i.e. interface of data acquisition and control, and personal computer, which executes procedure for generation, acquisition and analysis of phosphorus on sample |
| WO2008101732A1 (en) * | 2007-02-23 | 2008-08-28 | Ese Embedded System Engineering Gmbh | Optical measuring instrument |
| EP3974811A1 (en) * | 2020-09-29 | 2022-03-30 | ProBiomics GmbH | Determination of parameters in biological fluids using fluorescence and absorption |
| WO2022069542A1 (en) * | 2020-09-29 | 2022-04-07 | Probiomics Gmbh | Determination of parameters in biological fluids |
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|---|---|---|---|
| FZDE | Dead |