CA2345023A1 - Peptides useful for reducing symptoms of toxic shock syndrome and septic shock - Google Patents
Peptides useful for reducing symptoms of toxic shock syndrome and septic shock Download PDFInfo
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- CA2345023A1 CA2345023A1 CA002345023A CA2345023A CA2345023A1 CA 2345023 A1 CA2345023 A1 CA 2345023A1 CA 002345023 A CA002345023 A CA 002345023A CA 2345023 A CA2345023 A CA 2345023A CA 2345023 A1 CA2345023 A1 CA 2345023A1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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Abstract
This invention relates to compositions and methods which provide protection against, or reduce the severity of toxic shock and septic shock from bacterial infections. More particularly it relates to peptides derived from homologous sequences of the family of staphylococcal and streptococcal toxins, which may be polymeric, and carrier-conjugates thereof. The invention also relates to serum antibodies induced by the peptides and carrier-conjugates and their use to prevent, treat, or protect against the toxic effects of most, if not all, of the staphylococcal and streptococcal toxins. The invention also relates to diagnostic assays and kits to detect the presence of staphylococcal and streptococcal toxins, or antibodies thereto. The invention also relates isolated and purified to nucleic acids encoding the peptides of the invention and transformed host cells containing those nucleic acids.
Description
o PEPTIDES USEFUL FOR REDUCING SYMPTOMS
OF TOXIC SHOCK SYNDROME AND SEPTIC SHOCK
RELATED APPLICATIONS
This is a continuation-in-part application of co-pending U.S. Application Serial No. 09/168,303 filed October 7, 1998, which is in turn a continuation-in-part of co-pending U.S. Application Serial No. 08/838,413 filed April 7, 1997. Pursuant to 35 USC 365, U.S.
APPlication Serial No. 09/168,303 is also a continuation-in-part of co-pending International Application PCT/US98/06663, filed April 1, 1998. The entire disclosure of U.S. Application Serial No.
08/838,413 and U.S. Application Serial No. 09/168,303 are incorporated herein by reference.
FIELD OF THE INVENTION
This invention relates to compositions and methods for protecting against, or reducing the severity, of toxic shock syndrome and septic shock from bacterial infections. More particularly it relates to peptides, which may be polymeric, and carrier-conjugates thereof, derived from homologous sequences of the family of staphylococcal and streptococcal pyrogenic toxins. The peptides of the invention are useful to prevent, treat, or protect against the toxic effects of bacterial toxins, including most, if not all, of the staphylococcal and streptococcal pyrogenic toxins.
These are also useful to induce serum antibodies and may also be useful in diagnostic assays.
The invention also relates to antibodies induced by the peptides and/or carrier-conjugates and their use to o prevent, treat, or protect against the toxic effects of bacterial toxins, including most, if not all, of the staphylococcal and streptococcal pyrogenic toxins.
The invention also relates to compositions and methods to protect against, or ameliorate the effects of, autoimmune diseases which are associated with, or are the result of, the presence of staphylococcal or streptococcal toxins.
The invention also relates to diagnostic assays and kits to detect the presence of staphylococcal and streptococcal pyrogenic toxins, or antibodies thereto.
The invention also relates to isolated and purified nucleic acids encoding the peptides of the invention and transformed host cells containing those nucleic acids.
BACKGROUND OF THE INVENTION
The pyrogenic exotoxins of Group A streptococci and the enterotoxins of Staphylococcus aureus, which are also pyrogenic exotoxins, constitute a family of structurally related toxins which share similar biological activities (I1, 13). The staphylococcal and streptococcal pyrogenic exotoxins also share significant amino acid homology throughout their sequences (11, 19, 40). This pyrogenic exotoxin family contains nine main toxin types, and several allelic variants (subtypes) have been described. Several studies have shown that the toxins share common motifs based on immunologic cross reactivity between the toxins (26, 27). They stimulate CD4+, CD8+ and y8+ T cells by a unique mechanism. These toxins share the ability to bind the ~3 chain variable region (Vp) elements on the lateral face of the T cell receptor (TCR) and simultaneously bind to o the lateral face of the class II major histocompatibility complex (MHC) of antigen presenting cells (Figure 1), causing an aberrant proliferation of specific T-cell subsets (3, 4, 12). This property of the toxins has labeled them as "superantigens" (36) since they do not interact with the MHC and TCR
molecules in the manner of conventional antigens (14, 18) and produce a massive proliferation of T cells.
The variability of the sequences in the TCR-binding region and within the MHC-II-binding regions most likely provides the different superantigen toxins their specificities for different Va molecules and variable affinities for MHC-II types.(69-70) The cross-linking of TCR with MHC-II molecules by superantigens causes a profound blastogenesis of lymphocytes and antigen-presenting cells. The resulting stimulation of leukocytes leads to a significant increase in cytokine production.
Monocytes stimulated with bacterial superantigens produce the Thl cytokines IL-2 and IFN-y and the anti-inflammatory cytokines IL-10 and IL-1 receptor antagonist. (71). T cells activated by superantigen stimulation produce IL-12. (72). Whole preparations of peripheral blood mononuclear cells containing lymphocytes and antigen-presenting cells elicited a wide range of inflammatory cytokines in significant amounts.
The generation of monocyte cytokines such as IL-1, IL-6, TNF-a, and TNF-(3 was dependent on the presence of T
cells. (73).
Costimulatory molecules important in conventional immune responses also play a significant role in the o response of immune cells to superantigens. The costimulatory T cell antigen, CD28, and its corresponding ligand on MHC-II-bearing cells, B7, contribute to superantigen mitogenicity. (74, 75).
Other costimulatory molecules, such as LFA-1/ICAM-1 and VLA-4/VCAM-1, also contribute to the activation of immune cells by superantigens.(76, 77). These immunostimulatory activities of superantigens are crucial to their ability to cause injury to the host.
The bacterial toxins cause a variety of syndromes in humans. Staphylococcal enterotoxins have been implicated in staphylococcal food poisoning (26), as well as toxic shock like syndromes (1). The gene sequences and deduced amino acid sequences of at least six staphylococcal enterotoxins ("SE"): A, B, C, D, E
and H, are known, i.e., SEA, SEB, SEC, SED, SEE, and SEH
(19, 23). The streptococcal pyrogenic exotoxins ("SPE") have been implicated in causing the symptoms of scarlet fever and toxic shock like syndrome (8, 20, 30). The sequences of three members of this family are known:
SPEA, SPEC, and SSA (5, 23, 35).
Toxic shock syndrome toxin (TSST-1) from S. aureus shares similar biological activity with the SE's and SPE's, however amino acid sequences of this toxin are significantly different from these two classes of toxins (2). Structural analysis suggests that, despite the differences in amino acid composition, the overall topology of TSST-1 and the SE/SPE family of toxins is similar (41). The molecular structure of SE's and SPE's has been determined by various methods. Reviews concerning the molecular structures are available (19, 62). Molecular evolution studies of the SE/SPE family of a toxins suggests that the toxins can be grouped into two main clades(34). All these toxins are highly resistant to denaturation by heat and to proteases. With the exception of TSST-1, they are soluble proteins of approximately 230 amino acids and have a central disulphide loop. In contrast TSST-1 has only 194 amino acids and does not possess any cysteines.
It has been suggested that the conservation of amino acids is important to maintaining the structure necessary for the biological activity of the toxins (32). Mutations constructed in various positions throughout the SPEA and SEB molecules were sufficient to inactivate biological activity (6, 15). Mutations at various points throughout the molecules often had different effects, su ggesting that functional activities could not be attributed to any one region of the toxins (7). These results suggested that a functional tertiary structure must be maintained. Chemical modifications of highly conserved histidine residues inactivated biologic activity (29). The high conservation of the disulfide loop in the SE's and SPE's suggests an important role in the structure of the SE/SPE family of toxins. Studies show the disulfide loop is required for mitogenic activity of SEA and SEB. Reduction of the disulfide loop inactivated T cell stimulatory activity, but did not affect MHC-II binding and stimulation of monocytes (54). Peptide cleavages within the loop had no effect on T-cell mitogenicity, however cleavage of conserved sequences outside the loop of SEA resulted in loss of mitogenic activity. The loop and conserved adjacent sequences appear to be associated with avidity of the toxins to the TCR, and do not contribute to the specificity of toxins for a particular Vp type (6) .
Residues determining TCR V~ specificity appear to be located within the carboxy-terminus of the SE/SPE toxins (59), while residues critical for MHC-II binding appear to be located in the amino-terminal region, and the central portion of the molecule near the disulfide loop (53). The disulfide loop and adjacent highly conserved sequences contribute to the structural integrity of the toxins, and serve to bring the TCR and MHC binding regions in functional proximity to each other (65).
The SEs are named for their ability to induce gastrointestinal illnesses upon oral intake of a few micrograms of the toxin. The clinical effect appears in IS 2 to 4 hours and is manifested by nausea and diarrhea.
These symptoms appear to be caused by leukotrienes and histamine released from mast cells. Additionally, both the staphylococcal and streptococcal exotoxins are implicated in gram-positive shock. Although superantigen-related septic shock appears to be primarily mediated by tumor necrosis factor (TNF)-a and interleukin (IL) 12, the contribution of other cytokines cannot be discounted. (78, 79, 80).
The physiologic response to superantigens is similar to septic shock induced by gram-negative endotoxin (lipopolysaccharide, LPS). In fact, LPS and superantigens can work synergistically to produce lethal toxic shock. (81, 82, 83). Toxic shock syndrome can be exacerbated by the synergistic effects of TSST-1 with the SE/SPE family of toxins. (84, 85). Superantigen stimulation of immune cells can exacerbate autoimmune syndromes by causing the expansion of autoreactive T
o cell subsets, upregulation of MHC-II expression, and the potentiation of cytotoxic T cell response (86, 87, 88, 89, 90, 91).
Toxic shock syndrome is a specific syndrome caused by either the Stapylococcal or Streptococcal organisms.
It is specifically caused by the toxins produced by these bacteria. Clinically it often occurs in young women and children and is characterized by a raised temperature, low blood pressure, a rash that eventually leads to skin loss especially on the palms and the soles and multi-organ involvement.
Septic Shock on the other hand involves both gram negative as well as gram positive organisms, occurs in all groups of patients especially the elderly and post-surgical. It has similar symptoms except for the lack of a skin losing rash. Both diseases have a high mortality-however there are many more cases of septic shock as compared to toxic shock. The term "septic shock" is used herein to describe hypotension and organ failure associated with bacterial infections.
"Toxic shock like syndrome" is the term previously used to describe the syndromes caused by staphyloccal and streptococcal pyrogenic bacterial exotoxins other than toxic shock syndrome toxin (TSST-1) from S. aureus.
Currently, the term "toxic shock syndrome" is used to describe the syndromes caused by TSST-1 and the other pyrogenic exotoxins, and is the terminology used hereinafter.
Toxic shock syndrome can be exacerbated by the synergistic effects of TSST-1 with the enterotoxin/pyrogenic toxin family of toxins (9, 25).
Gram negative bacterial endotoxin and the pyrogenic _g_ o toxins can work synergistically to produce intractable shock ( 17 , 3 0 ) .
With respect to septic shock, lipopolysaccharide (LPS) is an integral part of the cell wall of Gram-negative bacteria and is a potent inducer of cytokine release by macrophages (52). During the induction phase of septicemia, LPS binds to the CD14 receptors of macrophages and triggers the release of a number of cytokines including Interleukin-1 (IL-1), and Tumor Necrosis Factor-a(TNF- a) (49). Accordingly, therapeutic strategies for septic shock have centered on the neutralization of LPS or LPS-induced cytokines (64).
Unfortunately, trials using either monoclonal antibodies directed against part of the LPS molecule or the use of CD14 soluble receptors have riot been very promising (45). The reasons for these failures might be: 1. The type of patient selected (many were already in irreversible shock). 2. The monoclonal antibody did not block all sites of LPS. 3. Soluble CD14 receptors did not block all LPS molecules.
Toxic shock syndrome and septic shock are still among the most life threatening syndromes affecting humans. It is estimated that approximately 20,000 cases of toxic shock syndrome occur each year of with a 10%
mortality rate (66). With respect to septic shock approximately 400,000-500,000 cases occur each year with a 50% mortality (63). Present therapy is primarily symptomatic with administration of fluids, antibiotics, pressor agents and occasionally steroids (56). There is no vaccine available for toxic shock syndrome since all of the superantigens are antigenically distinct even though there is some sequence homology present in all WO 00!10598 PCT/US99/22180 o the superantigens. There have been numerous vaccine trials for septic shock none of which have been successful.
With respect to the failed vaccine trials for septic shock, we believe that there was a failure to recognize that the interaction between the superantigens described above and LPS enhances the lethal potency of both these antigens by about 1000 fold. In contrast, each antigen when given alone requires a much higher dose for lethal septic shock (46).
Hence, it is proposed that at least two independent pathways of lethal septic shock can occur. LPS and peptidoglycan interact with macrophages. The superantigens interact with T cells. In both cases target cells are induced to release large amounts of cytokines. There is increasing evidence that gram-positive infections frequently accompany gram-negative infections in patients with septic shock (see article by Range!-Frausto, pages 299-312) (96). Exposure to gram-negative endotoxin produces a state of macrophage hyperesponsiveness on subsequent stimulation (92). A
similar state is seen with monocytes in septic shock.
Our group and others have shown that LPS and superantigens can act synergistically to produce lethal septic shock in animal models (93). It is our hypothesis that a significant amount of septic shock involves an early gram-negative infection that causes significant symptoms of vasodilation and hypotension.
This is then treated with fluids and antibiotics, leading to early recovery by the patient. Some days later, a gram-positive insult either via a line sepsis °f the skin or gastrointestinal flora may cause severe o irreversible shock in a previously LPS-sensitized patient. This model is depicted graphically in Figure 2 herein (94).
In other words, since Gram-negative and Gram-positive organisms can be recovered from patients with sepsis, it appears that it is the "two hit" hypothesis that is operative and the interaction between LPS and the superantigens markedly enhances the lethal properties of both molecules. In this model, the interruption of the toxin pathway by anti-peptide antibody(ies) or by peptides) of the invention prevents the onset of lethal shock induced by the combination of the LPS and one or more of the superantigens.
SUMMARY OF THE INVENTION
The present invention relates to the identification of consensus sequences derived from two conserved regions of the staphylococcal enterotoxins and streptococcal pyrogenic toxins (hereinafter called "region 1" and "region 2") and the discovery that compositions comprising amino acid sequences based on these two conserved regions of the staphylococcal enterotoxins and streptococcal pyrogenic exotoxins are capable of inducing antibodies which react with a variety of staphylococcal and streptococcal pyrogenic exotoxins and are also capable of ameliorating or preventing diseases related to the deleterious effects of these toxins.
The invention also relates to compositions and methods for preventing and treating diseases related to the release of certain pyrogenic exotoxins from bacteria.
o This invention provides peptides comprising amino acid sequences which reduce, inhibit or eliminate the deleterious effects of bacterial toxins and/or axe capable of inducing antibodies that reduce, inhibit or eliminate the deleterious effects of bacterial toxins, S such as those of staphylococcus and a variety of streptococci. Antibodies may be induced by administration of a pharmaceutical composition and/or vaccine containing a composition comprising a peptide 1~ derived from one or both of the two conserved regions described herein, or a structurally and/or immunologically related antigen.
The amino acid sequences provided by this invention are sufficiently common to all members of this family of pyrogenic exotoxins to be useful for eliciting antibodies which are cross-reactive with toxins derived from various bacteria.
The amino acid sequences provided by this invention 20 are also useful for new methods of preventing and treating symptoms associated with the bacterial release of the staphylococcal enterotoxins and the streptococcal pyrogenic exotoxins. Such methods include, for example, administering to an individual who is suspected of having an infection or developing and/or having a toxic or septic reaction, a compound comprising at least one of the consensus amino acid sequences of this invention in an amount sufficient to inhibit superantigen 30 stimulation of T-cells, preferably an amount sufficient to reduce, inhibit or eliminate the deleterious effects of the exotoxins. Such methods also include administering to an individual at risk of infection or 3S developing a toxic reaction to the exotoxins at least 0 one of the consensus amino acid sequences of this invention in an amount sufficient to elicit the production of antibodies to the exotoxins.
In a preferred embodiment of this invention, an individual at risk for developing toxic or septic shock syndrome or an individual with symptoms of toxic shock syndrome or septic shock may be treated by administering to such individual a composition comprising at least one of the peptides of this invention and/or carrier-conjugate thereof.
In another preferred embodiment of this invention, an individual at risk for developing toxic shock syndrome or septic shock, or an individual with symptoms of toxic shock syndrome or septic shock, may be treated by administering to such individual antibodies which have been generated in a mammal immunized with at least one of the compositions of this invention.
Vaccines and pharmaceutical compositions comprising at least one of the consensus amino acid sequences and a physiologically acceptable carrier and optionally an adjuvant are also part of this invention.
Another object of the invention is to provide antibodies induced by the peptides and carrier-conjugates thereof. These antibodies may be used to prevent, treat, or protect against the toxic effects of most, if not all, of the staphylococcal and streptococcal pyrogenic exotoxins. The antibodies may also be useful to protect against, or ameliorate the effects of, autoimmune diseases which are associated with, or are the result of, the presence of staphylococcal or streptococcal pyrogenic exotoxins.
These antibodies are also useful in diagnostic assays o and kits to detect the presence of staphylococcal and streptococcal pyrogenic exotoxins and to aid in the diagnosis of diseases related to the presence of those toxins.
Another object of the invention is to provide isolated and purified nucleic acids encoding the amino acid sequences of the invention, as well as suitable expression systems, vector components and transformed host cells containing those nucleic acids.
BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1. Schematic diagram of the interaction between a T cell receptor, superantigen, and a class II
MHC molecule. Superantigens bind to common sequences in class II MHC molecules and T cell receptors that lie outside the normal antigen-binding sites. T cell activation by superantigens is not limited by the antigenic specificity of the T cell.
Figure 2. Diagram of the "two hit" model of septic shock.
Figure 3. Comparison of the synthetic peptide sequences to conserved regions 1 and 2 of the staphylococcal enterotoxins (SEA, SEB, SEC, SED, SEE, and SEH), and sire tococcal p pyrogenic exotoxins (SPEA, SPEC, and SSA). Staphylococcal toxic shock syndrome toxin 1 (TSST-1) was compared with the region 2 peptide.
Numbers represent the residue positions as a reference to where these regions exist in the whole toxin molecules. Sequences are from either the Swiss protein or GenBank databases under the following accessian numbers. Swiss protein: SPEA, P08095; SPEC, P13380;
SEA, P13163; SEB, P01552; SEC, P01553; SED, P20723; SEE, o P12993. GenBank: SEH, U11702; SSA, L29565; TSST1, J02615.
Figure 4. ELISA titers of antibodies from rabbits immunized with polymeric peptide #6348. The peptide was diluted so that it was delivered to each well to give a final concentration of 2 ~,g/100 ~1. The serum was then diluted to 1:1,000; 1:10,000; 1:100,000; 1:500,000; and 1:1,000,000 and 100 ~1 of each dilution of serum was placed in each well. Experiments were run in triplicate for each dilution of serum. Note the 1 log higher titers of rabbit #443 serum as compared to rabbit #442 serum. Cut off readings were at O.D. 0.6.
Figure 5. 12% SDS PAGE gel immunoblot of a variety of staphylococcal and streptococcal toxins developed with the anti-peptide 6348 antibody. Note bands of correct molecular weight (M. W.) of each toxin identified by the anti-peptide antibody. Lane 1: SPEA, lane 2:
SEA, lane 3: SEB, lane 4: SED, lane 5: SEE, lane 6: SEC
and lane 7 TssT-1. Note bands at appropriate M.W. in lanes 1-4. Fainter bands are seen in lanes 5 and 7.
Figure 6. Bar graphs of blastogenesis assays of human mononuclear cell populations stimulated by various toxins in the presence of normal rabbit serum and anti-peptide 6348 serum. Note the marked inhibition of SEB, SEC, SEE, SPEA and SPEC by the anti-peptide antibody. Less, but definite, inhibition of SEA by the anti-peptide antibody was also seen.
Fi ure 7. Bar g graphs of blastogenesis assays of human mononuclear cell populations stimulated by SEB in the presence of (A) peptide 6343 (i.e., CMYGGVTEHEGN, SEQ ID N0:3),(B) peptide 6346 (i.e., CGKKNVTVQELDYKIRKYLVDNKKLYGC, SEQ ID NO: 6)) and (C) o peptide 6348 (i.e., CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC, SEQ ID N0:8).
Figure 8. Inhibition of SEB, SEC, SED, SPEC, SPEA
and TSST-1 toxin blastogenesis of peripheral blood mononuclear cells (PBMC) by the 6343 peptide. 2 x 105 PBMC were stimulated with either 2~g of the indicated toxin or a combination of 2~,g of the toxin with 150~.g of the 6343 peptide. These were incubated for 72 hours and the results were measured via tritiated thymidine incorporation. CPM represents counts per minute. Note that the single peptide (6343) inhibited all of the superantigens tested.
Figure 9. Inhibition of SPEG, SPEH, and SPEZ toxin blastogenesis of peripheral blood mononuclear cells (PBMC) by the 6343 peptide. 2 x 105 PBMC were stimulated with either 2~.g of the indicated toxin or a combination of 2~g of the toxin with the indicated amount of the 6343 peptide. These were incubated for 72 hours and the results were measured via tritiated thymidine incorporation. CPM represents counts per minute. Normal represents normal media. Note that the single peptide (6343) inhibited the superantigens SPEG, SPEH and SPEZ.
Figure Z0. (A). Binding of peptide 6343 to the MHC complex as measured by ELISA. (B). Inhibition of binding of SEB toxin biding by peptide 6343 as measured by decreased anti-SEB binding at increased concentrations of added peptide 6343.
Figure 11. Confocal microscope pictures. (A) Binding of peptide 6343 is indicated by the green color.
(B) Binding of anti-MHC peptide is indicated by the red color. (C) Combined picture showing stippled pattern of o red and green color. Binding of peptide 6343 is indicated by the green color and binding of anti-MHC
peptide is indicated by the red color.
DETAILED DESCRIPTION OF THE INVENTION
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only, and are not restrictive of the invention, as claimed. The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate an embodiment of the invention and, together with the description, serve to explain the principles of the invention.
Two consensus patterns, corresponding to conserved region 1 and region 2, respectively, are identified as common to members of the staphylococcal enterotoxin and streptococcal pyrogenic toxin family of toxins when the Program "Motifs" in a software package from the Genetics Computer Group, Inc. ("GCG") is run using the streptococcal SPEC toxin as an example. "Program Manual for the Wisconsin Package, Version 8, September 1994, Genetics Computer Group, 575 Science Drive, Madison, Wisconsin USA 53711"
incorporated herein by reference.
The first consensus sequence ("GCG consensus #1") identified by the Motifs program has the amino acid sequence YGG(LIV)TXXXXN, which is rewritten herein as YGGX1TX2X3X4XSN (SEQ ID NO:1) , wherein X1 is selected from the group consisting of L, I, or V; and X2, X3, X4 and XS
are each independently selected from the group consisting of any amino acid. This pattern is present in the staphylococcal enterotoxins and streptococcal o pyrogenic exotoxins, but not in TSST-1. The sequence begins immediately at the COOH-terminal side of the cysteine loop. The second consensus sequence ("GCG
consensus #2") identified by the Motifs program has the amino acid sequence KXX(LIV)XXXX(LIV)DXXXRXXLXXXXX(LIV)Y, rewritten herein aS KX6X~XgXgX1pX11X12X13DX14X15X16RX17X18LX19X20X21X22X23X24Y (SEQ
ID NO: 2) , wherein X8, X13 and X24 are each independently selected from the group consisting of L, I and V, and X6r X7r X9r XlOr Xllr Xl2r X14 XlSr Xl6r Xl7r XlBr Xl9r X20r Xzl, X22 and X23 are each independently selected from the group consisting of any amino acid. This pattern is present in the staphylococcal enterotoxins, streptococcal pyrogenic exotoxins, and TSST-1.
One object of the invention is to provide compositions comprising peptides comprising amino acid sequences based on these two conserved regions of the staphylococcal enterotoxins and streptococcal pyrogenic toxins. These peptides may be used for eliciting an immunogenic response in mammals, including responses which provide protection against, or reduce the severity, of toxic shock or septic shock from staphylococcal or streptococcal infections. These peptides may also be useful to protect against, or ameliorate the effects of, autoimmune diseases which are associated with, or are the result of, the presence of staphylococcal or streptococcal pyrogenic exotoxins.
These peptides are also useful in diagnostic assays and kits to detect the presence of antibodies to staphylococcal and streptococcal pyrogenic exotoxins and to aid in the diagnosis of diseases related to the Presence of those toxins.
o The peptides of the invention are those derived from either one or both of the following two consensus sequences:
YGGX1TX2X3X4X5N (SEQ ID NO:l) , wherein X1 is selected from the group consisting of L, I, or V; and X2, X3, X4 and XS
are each independently selected from the group consisting of any amino acid.
KXX(LIV)XXXX(LIV)DXXXRXXLXXXXX(LIV)Y, rewritten herein aS KX6X7XgX9X1pX11X12X13DX14X15X16RX17X18LX19X20X21X22X23X24Y ( SEQ
ID NO: 2) , wherein X8, X13 and X24 are each independently selected from the group consisting of L, I and V, and X6i X7i X9i XlOi Xlli Xl2i Xl4i XlSi Xl6i Xl7i XlBi Xl9i X201 X21, X2z and X23 are each independently selected from the group consisting of any amino acid.
A preferred consensus sequence of the invention from Region 1 (consensus #la) has the amino~acid sequence X25X2sYGGXITX2X3X4X5N (SEQ ID NO: 28) , wherein X1 is selected from the group consisting of L, I, and V;
X2. X4 and XS are each independently selected from the group consisting of any amino acid; and X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; but preferably X1 is selected from the group consisting of I
and V; X2 is selected from the group consisting of L, E, K, P and N; X3 is selected from the group consisting of H and A and no amino acid; X4 is selected from the group consisting of D, N, E, Q, and H; XS is selected from the group consisting of N, G, S, and R; X25 is selected from the group consisting of C and Y and no amino acid; and X26 is selected from the group consisting of M, T, L, I, and no amino acid.
A Preferred consensus sequence of the invention o from region 2 (consensus ##2a) has the amino acid sequence : KX6X~XgXgX1pX11X12X13DX14Xi5X16RX17X18X27Xy9X20X21 X22X23X24Y (SEQ ID NO: 29) , wherein Xa, X13 and X24 are each independently selected from the group consisting of L, I
and V; X6, X~, Xs. Xlo~ X11. X12 Xla~ Xis~ Xls. Xl~. Xls Xls.
X2o, X21. X22, and X23 are each independently selected from the group consisting of any amino acid; and X2~ is selected from the group consisting of L and Y; but preferably X6 is selected from the group consisting of K
and D; X, is selected from the group consisting of N, K, S, E, M, I and Q; X$ is selected from the group consisting of L and V; X9 is selected from the group consisting of T and A; Xlo is selected from the group consisting of V, A, L, F and I; X11 is selected from the group consisting of Q and S; X12 is selected from the group consisting of E and T; X13 is selected from group consisting of L and I; X14 is selected from the group consisting of L, Y, I, A, F and C; Xls is selected from the group consisting of Q, L, K and E; X16 is selected from the group consisting of A, T, I and V; X1-, is selected from the group consisting of R, H, N and K; X18 is selected from the group consisting of Y, F, I, L and Q; X19 is selected from the group consisting of Q, V, I, H, S, T and M; X2o is selected from the group consisting of E, K, N, G, D, S and Q; X21 is selected from the group consisting of K, N, D, R and I; X22 is selected from the group consisting of Y, K, L, F and H; X23 is selected from the group consisting of N, K, G and Q; X24 is selected from the group consisting of L and I; and X2~ is L.
The following Table 1 lists the amino acids that are found at each of the variable positions in the o sequences shown in Figure 3, and the number of times they appear at that position:
Table Frequency of the ids in riablepositions amino the va ac in the sectuences shown in gure 3 Fi Xz 3L 2E 1K 2P 1N
X3 7H lA one deletion (no amino acid) Xs 3N 4G 1S 1R
Xs 9K 1D
X~ 3N 1K 1S lE 1M lI 1Q
Xa 9V 1L
X9 9T lA
Xlo 4V 3A 1L 1F lI
X11 9Q 1s X13 9L lI
Xls 3Q 1L 5K lE
Xl~ 2R 3H 1N 4K
X19 2Q 2V lI 1H 1S 2T 1M
X2o lE 2K 1N 1G 3D 1S 1Q
X21 4K 3N 1D 1R lI
Xz4 8L 2I
X2s 8C lY
X2~ 9L lY
o In the peptides of the present invention, X1, Xe, X13 and X24 may each independently be selected from the group consisting of L, I and V; X2, X3, X4, Xs, X6, X7, X9. X10. X11. X12. X14. Xls. X16. X17. Xle X19. X201 X21. X22.
Xz3. Xzs and X26 may each independently be any amino acid;
X3. Xzs and X26 may also each independently be no amino acid; and X27 is selected from the group consisting of L
and Y. However, in general, the amino acids present at the positions X1 to X27 in the toxins shown in Figure 3 (and listed in Table 1) are preferred for those positions, and the amino acids present most often at those positions in the toxins shown in Figure 3 (and listed in Table 1) are more preferred. For example, from Figure 3, and Table 1, it can be determined that H
(histidine) is present in seven toxins at position X3 and A (alanine) is present in one toxin at position X3, and there is no amino acid present in one toxin at X3.
These are the preferred amino acids for position X3.
The more preferred amino acid for position X3 in a peptide of the invention is H (histidine). The more preferred amino acids for X1 through X26 are: X1 =
valine; X2 = leucine; X3 = histidine; X4 = glutamic acid;
Xs = glycine; X6 = lysine; X7 = asparagine; X8 = valine;
Xs = threonine; Xlo = valine; X11 = glutamine; X12 =
glutamic acid; X13 - leucine; X14 = leucine, tyrosine, isoleucine or phenylalanine; X15 = lysine; X16 = alanine;
X17 = lysine; X18 = tyrosine; X19 = glutamine, valine or threonine; X2o = aspartic acid; Xzl = lysine; X22 =
lysine; X23 = lysine; X2g = leucine; X25 = cysteine; X26 =
methionine; and X27 = leucine. But note that in the exemplified peptides of the invention described hereinbelow, i.e., SEQ ID NOS: 6, 7 and 8, inosine (I) o is used at position X16 instead of the more frequently found alanine (A).
As is evident from Figure 3 and the above Table 1, some amino acid residues are much more highly conserved than suggested by the GCG package data provided by the "Motifs" program.
In region 1, the preferred consensus is larger (consensus #la), and usually includes a C in the first position (X25) . The second residue (X26) is most often a M, but this can vary. In the ninth position (X3), H is the most highly conserved. The eleventh residue (XS) is most often a G.
In region 2, the preferred consensus (consensus #2a) is much more highly conserved than suggested by the GCG program, especially if one excludes TSST-1 sequences from consideration, as follows: The second position (X6) is more highly conserved than suggested, being almost exclusively a K; the fourth residue (X8) is always a V followed exclusively by a T in the fifth position (X9); the sixth position (Xlo) is somewhat variable; but the seventh position (X11) is always a Q, followed by E (X12). The next position is almost always an L (X13) , and the second to last position (X24) is almost always an L.
Thus, additional modified consensus sequences for region 1 and region 2, which are of narrower scope than the GCG consensus sequences #1 and #2 and the modified consensus sequences #la and #2a, are as follows:
o Consensus #lb:
CMYGGX1TX2HX4GN (SEQ ID NO: 30) wherein X1 is V or I, preferably V;
X2 is L, E, K, P or N, preferably E or L;
and X4 is D, N, E, Q or H, preferably E.
Consensus #2b:
KKX~VTXIOQELDX14X1sX1sRXI~XleXz~XzsX2oXz1Xa2Xa3LY (SEQ ID
N0:31) wherein X~ is N, K, S, E, M, I or Q, preferably N;
X10 is V, A, L, F or I, preferably V;
X14 is L, Y, I, A, F or C, preferably Y;
Xls is Q, L, K or E, preferably K;
Xls is A, T, I or V, preferably I;
Xl~ is R, H, N or K, preferably K;
X18 is Y, F, I, L or Q, preferably Y;
X19 is Q, V, I, H, S, T or M, preferably V;
X2o is E, K, N, D, G, S or Q, preferably D;
X21 is K, N, D, R or I, preferably N;
X22 is Y, K, L, F or H, preferably K;
X23 is N, K, G or Q, preferably K; and X2~ is L or Y, preferably L.
Peptides exemplified herein are CMYGGVTEHEGN (SEQ
ID NO: 3), CMYGGVTEHEGNGC* (SEQ ID NO: 5), KKNVTVQELDYKIRKYLVDNKKLY (SEQ ID N0: 4), CGKKNVTVQELDYKIRKYLVDNKKLYGC* (SEQ ID NO: 6), CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLY (SEQ ID NO: 7) and CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC* (SEQ ID NO: 8), wherein an asterisk indicates that the peptide is a randomly cross-linked polymer. The exemplified polymer o peptides are at least 6,000 to 8,000 daltons. The average size of the exemplified polymer peptides is about 12,000 to 15,000 daltons. Small peptides and/or contaminants may be removed by dialysis or other methods available in the art. Similarly, larger aggregates may be removed usin g, e.g., a 0.25 micron filter, which can also be used to sterilize the peptides.
Note that the amino acids cysteine and methionine, "CM", are present at the amino terminus of the exemplified region 1 peptides since those amino acids are most often found in that position in nature. Note also that the amino acids cysteine and glycine, "CG" and "GC", are used at the amino and/or carboxy- termini of some of the exemplified region 2 peptides. The amino acid cysteine "C" is used to facilitate cross-linking through the formation of disulfide bonds. The amino acid glycine, "G", is used as a spacer residue.
The preferred peptides of the invention are those which exclude full length native toxin molecules. The preferred peptides of this invention are not toxic, but toxic peptides maybe useful in this invention, for example, in eliciting antibodies in a non-human system.
The most preferred peptides of the invention do not contain amino acid sequences in the sequence in which they are found in any particular native toxin molecule.
The present invention encompasses monomers of the peptides derived from either one or both of the two consensus regions described herein. These monomers may comprise one or more sequences derived from either region 1 or region 2 or both, such as consensus sequences #1 and #2, preferably consensus sequences #la and/or #2a, more preferably consensus sequences #lb o and/or #2b, most preferably one or more of the exemplified consensus sequence peptides. If the monomer contains more than one consensus sequence, these sequences may be immediately adjacent to each other or separated by a linker. In addition, different orientations of the peptides are within the scope of this invention. Furthermore, the order of the consensus peptides within the full peptide may be variable.
The present invention also encompasses homogeneous or heterogeneous polymers of the peptides disclosed herein (e. g., concatenated, cross-linked and/or fused identical peptide units or concatenated, cross-linked and/or fused diverse peptide units), and mixtures of the peptides, polymers, and/or conjugates thereof.
Linkers useful in the invention may, for example, be simply peptide bonds, or may comprise amino acids, including amino acids capable of forming disulfide bonds, but may also comprise other molecules such as, for example, polysaccharides or fragments thereof.
In the peptides exemplified herein, sequences derived from consensus region 1 and consensus region 2 may be immediately adjacent to each other, linked by peptide bonds, (see, era., SEQ ID N0:7) and/or connected via amino acid linkers capable of forming di-sulfide bonds via cysteine residues (see, e-a., SEQ ID N0: 8).
In the native toxin molecules, the sequences of region 1 and region 2 are separated by about 27 amino acids.
When the linkers are additional amino acids, they are most preferably 1 to 27 amino acids in length, although longer linkers may also be used in accordance with this invention.
The linkers for use with this invention may be o chosen so as to contribute their own immunogenic effect which may be either the same, or different, than that elicited by the consensus sequences of the invention.
For example, such linkers may be bacterial antigens which also elicit the production of antibodies to infectious bacteria. In such instances, for example, the linker may be a protein or protein fragment of an infectious bacteria, or a bacterial polysaccharide or polysaccharide fragment.
A peptide of the invention includes any substituted analog or chemical derivative of a peptide derived from one or both of the two consensus regions described herein, most preferably of the exemplified peptides described herein, so long as the peptide is capable of inhibiting binding of staphylococcal and streptococcal pyrogenic exotoxins to the MHC complex; inhibiting blastogenesis of human mononuclear cells in the presence of any one of the toxins; eliciting the production of antibodies capable of binding to most of the staphylococcal and streptococcal pyrogenic exotoxins; or reacting with (i.e., specifically binding to) antibodies that react with most of the staphylococcal and streptococcal pyrogenic exotoxins. Therefore, a peptide can be subject to various changes that provide for certain advantages in its use. For example, D amino acids can be substituted for L amino acids to increase in vivo stability of the peptides, while still retaining biological activity. See, e~a., Senderoff et al. (1998) (95). Likewise, retro-inverso peptides, which contain NH-CO bonds instead of CO-NH peptide bonds, are much more resistant to proteolysis than L-peptides.
Moreover, they have been shown to mimic natural L-o peptides with respect to poly- and monoclonal antibodies (48). Therefore, peptides having at least one D amino acid on the amino terminal and/or carboxy terminal end of the molecule and which retain biological activity are considered part of the invention. In addition, retro-inverso peptides which contain one or more of the amino acid sequences of the invention and which retain biological activity are also considered part of the invention.
The peptides of the invention are useful for providing active immunization for the prevention of disease related to the deleterious effects of staphylococcal and streptococcal pyrogenic exotoxins and for preparation of antibodies as a passive immunization therapy. When used to prepare antibodies, the peptides are designed to induce antibodies which react with a variety of staphylococcal and streptococcal pyrogenic exotoxins (preferably with at least two, more preferably with at least four, and most preferably with at least seven of the pyrogenic exotoxins) for use in therapy to increase resistance to, prevent and/or treat toxic shock syndrome and septic shock.
The peptides may also be useful to protect against, or ameliorate the effects of, autoimmune diseases which are associated with, or are the result of, the presence of staphylococcal or streptococcal exotoxins.
The peptides of the invention will also be useful in diagnostic tests for detecting antibodies to staphylococcal and streptococcal pyrogenic exotoxins.
The peptide may be mixed with an adjuvant. The peptide also may be bound to a non-toxic non-host Protein carrier to form a conjugate or it may be bound o to a saccharide carrier and/or a non-toxic non-host protein carrier to form a conjugate.
The molecular weight of the peptide monomers having one consensus sequence of the invention range from about 1000 to 5000 daltons. Such lower molecular weight species of the invention are useful themselves to inhibit superantigen induced T cell proliferation and/or reduce, inhibit or eliminate the deleterious effects of bacterial exotoxins in vivo, either when used alone or in combination with another form of therapy, e.g., anticytokine antibodies.
Such lower molecular weight species of the invention may also be useful as immunogens themselves or, more preferably, may be used as haptens conjugated to a larger carrier molecule, such as, for example, a protein. As with other peptides, the molecular weight of the peptide alone, or when conjugated to a carrier, or in the presence of an adjuvant, is related to its immunogenicity. Thus, the peptide may vary in molecular weight in order to enhance its antigenicity or immunogenicity. In an exemplified embodiment, the molecular weight of the peptide, in polymeric form, is greater than about 6000 to 8000 daltons, with an average weight of 12,000 to 15,000 daltons. The total size of the peptide is only limited to its ability to be physiologically tolerated.
The invention also relates to isolated and purified nucleic acid molecules which code for the peptides of the invention to produce the encoded peptides. The encoded peptides may be monomers, polymers or linked to other peptide sequences (e. g., they may be fusion proteins). Other features of the invention include WO 00/20598 PC'T/US99/22180 o vectors which comprise the nucleic acid molecules of the invention operably linked to promoters, as well as cell lines, such as prokaryotic (e.g., E. coli) and eukaryotic (e. g., CHO and COS) cells transfected with the nucleic acid molecules of the invention. Vectors and compositions for enabling production of the peptides in vivo, i.e., in the individual to be treated or immunized, are also within the scope of this invention.
The nucleic acids encoding the peptides of the invention can be introduced into a vector such as a plasmid, cosmid, phage, virus or mini-chromosome and inserted into a host cell or organism by methods well known in the art. In general, the vectors containing these nucleic acids can be utilized in any cell, either eukaryotic or prokaryotic, including mammalian cells (e. g., human (e. g., HeLa), monkey (e. g., COS), rabbit (e.g., rabbit reticulocytes), rat, hamster (e.g., CHO
and baby hamster kidney cells) or mouse cells (e.g., L
cells), plant cells, yeast cells, insect cells or bacterial cells (e.g., E. coli). The vectors which can be utilized to clone and/or express these nucleic acids are the vectors which are capable of replicating and/or expressing the nucleic acids in the host cell in which the nucleic acids are desired to be replicated and/or expressed. See, e.g., F. Ausubel et al., Current Protocols in Molecular Biolocrv, Greene Publishing Associates and Wiley-Interscience (1992) and Sambrook et al. (1989) for examples of appropriate vectors for various types of host cells. Strong promoters compatible with the host into which the gene is inserted may be used. These promoters may be inducible. The host cells containing these nucleic acids can be used to o express large amounts of the protein useful in pharmaceuticals, diagnostic reagents, vaccines and therapeutics.
The nucleic acids could be used, for example, in the production of peptides for diagnostic reagents, vaccines and thera ies for p pyrogenic exotoxin related diseases. For example, vectors expressing high levels of peptide can be used in immunotherapy and immunoprophylaxis, after expression in humans. Such vectors include retroviral vectors and also include direct injection of DNA into muscle cells or other receptive cells, resulting in the efficient expression of the peptide, using the technology described, for example, in Wolff et al., Science 247:1465-1468 (1990), ~"~olff et al. , Human Molecular Genetics 1 (6) :363-369 (1992) and Ulmer et al., Science 259:1745-1749 (1993).
See also, for example, WO 96/36366 and WO 98/34640.
In another embodiment of this invention antibodies are provided which react with peptides of the invention, as well as a variety of staphylococcal and streptococcal pyrogenic exotoxins (preferably with at least two, more preferably with at least four, and most preferably with at least seven of the pyrogenic exotoxins). These antibodies will be useful for passive immunization therapy to increase resistance to or prevent toxic shock syndrome or septic shock or other diseases related to the presence of bacterial pyrogenic exotoxin. The antibodies may also be useful to protect against, or ameliorate the effects of, autoimmune diseases which are associated with, or are the result of, the presence of staphylococcal or streptococcal pyrogenic exotoxins.
The antibodies of the invention will also be useful in o diagnostic tests and kits for detecting the presence of staphylococcal and streptococcal pyrogenic exotoxins.
These uses are discussed in more detail below.
Methods for t~reparincr peptides of the invention The peptides of the invention may be prepared by synthetic methods or by recombinant DNA methods, as known in the art and as described herein.
Pharmaceutical Compositions The pharmaceutical compositions of this invention contain a pharmaceutically and/or therapeutically effective amount of at least one peptide and/or carrier thereof, antibody, or nucleic acid encoding a peptide of this invention. In one embodiment of the invention, the effective amount or peptide per unit dose is an amount Sufficient to inhibit T-cell proliferation by staphylococcal and/or streptococcal pyrogenic exotoxins.
In another embodiment of the invention, the effective amount of peptide per unit dose is an amount sufficient to prevent, treat or protect against the toxic effects of bacterial toxins, including diarrhea and/or cardiopulmonary depression or lethal shock. The effective amount of peptide per unit dose depends, among other things, on the species of mammal inoculated, the body weight of the mammal and the chosen inoculation regimen, as is well known in the art.
In such circumstances, inocula for a human or similarly sized mammal typically contain peptide concentrations of 100 to 500 mgs/kg, body weight of the mammal per inoculation dose.
Preferably, the route of inoculation of the peptide will be subcutaneous or intravenous. The dose is administered at least once.
o When the peptide of the invention is used as immunogen, the pharmaceutical composition contains an effective, immunogenic, amount of peptide of the invention. The effective amount of peptide per unit dose sufficient to induce an immune response depends, among other things, on the species of mammal inoculated, the body weight of the mammal and the chosen inoculation regimen, as well as the presence or absence of an adjuvant, as is well known in the art. Inocula typically contain peptide concentrations of about 1 microgram to about 1000 micrograms per inoculation (dose), preferably about 3 micrograms to about 100 micrograms per dose, most preferably about 5 micrograms to 50 micrograms. The use of higher amounts is envisaged. In Example 1, rabbits were injected twice with 500 micrograms of polymeric peptide in the presence of adjuvant. In Example 5,an example in which the peptide is administered directly to prevent toxic or septic shock, which may not be dependent on the production of antibodies, mice were injected twice with 1.5 mg of monomer peptide for a total of 3 mgs.
Standard procedures to determine dose response relationships known to those skilled in the art may be used to determine optimum doses of peptide to be used either to prevent or treat toxic or septic shock, or to raise antibodies for its prevention or treatment.
The term "unit dose" as it pertains to the inocula refers to physically discrete units suitable as unitary dosages for mammals, each unit containing a predetermined quantity of active material (e. g., peptide, antibody or nucleic acid) calculated to produce the desired immunogenic effect in association with the o required diluent.
Inocula are typically prepared as a solution in a physiologically acceptable carrier such as saline, phosphate-buffered saline and the like to form an aqueous pharmaceutical composition.
The peptides of the invention are generally administered with a physiologically acceptable carrier or vehicle therefor. A physiologically acceptable carrier is one that does not cause an adverse physical reaction upon administration and one in which the antibodies are sufficiently soluble and retain their activity to deliver a therapeutically effective amount of the compound. The therapeutically effective amount and method of administration of a peptide of the invention may vary based on the individual patient, the indication being treated and other criteria evident to one of ordinary skill in the art. A therapeutically effective amount of a peptide of the invention is one sufficient to attenuate the dysfunction without causing significant side effects such as non-specific T cell lysis or organ damage. The routes) of administration useful in a particular application are apparent to one or ordinary skill in the art.
Routes of administration of the peptides include, but are not limited to, parenteral, and direct injection into an affected site. Parenteral routes of administration include but are not limited to intravenous, intramuscular, intraperitoneal and subcutaneous. The route of inoculation of the peptides of the invention is typically parenteral and is preferably intramuscular, sub-cutaneous and the like.
The present invention includes compositions of the o peptides described above, suitable for parenteral administration including, but not limited to, pharmaceutically acceptable sterile isotonic solutions.
Such solutions include, but are not limited to, saline and phosphate buffered saline for nasal, intravenous, intramuscular, intraperitoneal, subcutaneous or direct injection into a joint or other area.
A system for sustained delivery of the peptides of the invention may also be used. For example, a delivery system based on containing a peptide in a polymer matrix of biodegradable microspheres may be used (57). One such polymer matrix includes the polymer poly(lactide-co-glycolide) (PLG). PLG is biocompatible and can be given intravenously or orally. Following injection of the microspheres into the body, the encapsulated protein is released by a complex process involving hydration of the particles and drug dissolution. The duration of the release is mainly governed by the type of PLG polymer used and the release of modifying excipients (4~).
The dose is administered at least once. When a composition of the invention is used to induce antibodies, at least one booster dose may be administered after the initial injection, preferably at about 4 to 6 weeks after the first dose, in order to increase the antibody level. Subsequent doses may be administered as indicated.
To monitor the antibody response of individuals administered the compositions of the invention, antibody titers may be determined. In most instances it will be sufficient to assess the antibody titer in serum or plasma obtained from such an individual. Decisions as .to whether to administer booster inoculations or to o change the amount of the composition administered to the individual may be at least partially based on the titer.
The titer may be based on either an immunobinding assay which measures the concentration of antibodies in the serum which bind to a specific antigen, i.e. peptide or toxin; or bactericidal assays which measure the ability of the antibodies to participate with complement in killing bacteria. The ability to neutralize in vitro and in vivo biological effects of the pyrogenic exotoxins may also be assessed to determine the effectiveness of the treatment. See, e.g., the examples herein.
Antibodies The term "antibodies" is used herein to refer to immunoglobulin molecules and immunologically active portions of immunoglobulin molecules. Exemplary antibody molecules are intact immunoglobulin molecules, substantially intact immunoglobulin molecules and portions of an immunoglobulin molecule, including those portions known in the art as Fab, Fab', F(ab')z and F(v) as well as chimeric antibody molecules.
An antibody of the present invention is typically produced by immunizing a mammal with an immunogen or vaccine containing one or more peptides of the invention, or a structurally and/or antigenically related molecule, to induce, in the mammal, antibody molecules having immunospecificity for the immunizing peptide or peptides. The peptides) or related molecules) may be monomeric, polymeric, conjugated to a carrier, and/or administered in the presence of an adjuvant. The antibody molecules may then be collected from the mammal if they are to be used in immunoassays 0 or for providing passive immunity.
The antibody molecules of the present invention may be polyclonal or monoclonal. Monoclonal antibodies may be produced by methods known in the art. Portions of immunoglobulin molecules may also be produced by methods known in the art.
The antibody of the present invention may be contained in various carriers or media, including blood, plasma, serum (e. g., fractionated or unfractionated serum), hybridoma supernatants and the like.
Alternatively, the antibody of the present invention is isolated to the extent desired by well known techniques such as, for example, by using DEAE Sephadex, or affinity chromatography. The antibodies may be purified so as to obtain specific classes or subclasses of antibody such as IgM, IgG, IgA, IgGl, IgG2, IgG3, IgG4 and the like. Antibody of the IgG class are preferred for purposes of passive protection.
The presence of the antibodies of the present invention, either polyclonal or monoclonal, can be determined by various assays. Assay techniques include, but are not limited to, immunobinding, immunofluorescence (IF), indirect immunofluorescence, immunoprecipitation, ELISA, agglutination and Western blot techniques.
The antibodies of the present invention have a number of diagnostic and therapeutic uses. The antibodies can be used as an in vitro diagnostic agent to test for the presence of various staphylococcal and streptococcal pyrogenic exotoxins in biological samples in standard immunoassay protocols and to aid in the diagnosis of various diseases related to the presence of o bacterial pyrogenic exotoxins. Preferably, the assays which use the antibodies to detect the presence of bacterial pyrogenic exotoxins in a sample involve contacting the sample with at least one of the antibodies under conditions which will allow the formation of an immunological complex between the antibody and the toxin that may be present in the sample. The formation of an immunological complex if any, indicating the presence of the toxin in the sample, is then detected and measured by suitable means. Such assays include, but are not limited to, radioimmunoassays, (RIA), ELISA, indirect immunofluorescence assay, Western blot and the like.
The antibodies may be labeled or unlabeled depending on the type of assay used. Labels which may be coupled to the antibodies include those known in the art and include, but are not limited to, enzymes, radionucleotides, fluorogenic and chromogenic substrates, cofactors, biotin/avidin, colloidal gold and magnetic particles. Modification of the antibodies allows for coupling by any known means to carrier proteins or peptides or to known supports, for example, polystyrene or polyvinyl microliter plates, glass tubes or glass beads and chromatographic supports, such as paper, cellulose and cellulose derivatives, and silica.
Such assays may be, for example, of direct format (where the labelled first antibody reacts with the antigen), an indirect format (where a labelled second antibody reacts with the first antibody), a competitive format (such as the addition of a labelled antigen), or a sandwich format (where both labelled and unlabelled antibody are utilized), as well as other formats WO 00/20598 PC'T/US99/22180 o described in the art. In one such assay, the biological sample is contacted to antibodies of the present invention and a labelled second antibody is used to detect the presence of staphylococcal and streptococcal pyrogenic exotoxins, to which the antibodies are bound.
The antibodies of the present invention are also useful as therapeutic agents in the prevention and treatment of diseases caused by the deleterious effects of staphylococcal and streptococcal pyrogenic exotoxins.
The antibodies are generally administered with a physiologically acceptable carrier or vehicle therefor.
A physiologically acceptable carrier is one that does not cause an adverse physical reaction upon administration and one in which the antibodies are sufficiently soluble and retain their activity to deliver a therapeutically effective amount of the compound. The therapeutically effective amount and method of administration of the antibodies may vary based on the individual patient, the indication being treated and other criteria evident to one of ordinary skill in the art. A therapeutically effective amount of the antibodies is one sufficient to inhibit superantigen stimulation of T-cells and/or attenuate the dysfunction caused by the presence of bacterial toxins without causing significant side effects such as non-specific T
cell lysis or organ damage. The routes) of administration useful in a particular application are apparent to one or ordinary skill in the art.
Routes of administration of the antibodies include, but are not limited to, parenteral, and direct injection into an affected site. Parenteral routes of administration include but are not limited to o intravenous, intramuscular, intraperitoneal and subcutaneous.
The present invention includes compositions of the antibodies described above, suitable for parenteral administration including, but not limited to, pharmaceutically acceptable sterile isotonic solutions.
Such solutions include, but are not limited to, saline and phosphate buffered saline for nasal, intravenous, intramuscular, intraperitoneal, subcutaneous or direct injection into a joint or other area.
Antibodies for use to elicit passive immunity in humans are preferably obtained from other humans previously inoculated with compositions comprising one or more of the consensus amino acid sequences of the invention. Alternativel y, antibodies derived from other species may also be used. Such antibodies used in therapeutics suffer from several drawbacks such as a limited half-life and propensity to elicit an immune response. Several methods have been proposed to overcome these drawbacks. Antibodies made by these methods are encompassed by the present invention and are included herein. One such method is the "humanizing" of non-human antibodies by cloning the gene segment encoding the antigen binding region of the antibody to the human gene segments encoding the remainder of the antibody. Only the binding region of the antibody is thus recognized as foreign and is much less likely to cause an immune response. An article describing such antibodies is Reichmann et al., "Reshaping Human Antibodies for Therapy", Nature 332:323-327 (1988), which is incorporated herein by reference. See also, Queen et al., US Patent 5,585,089, which is incorporated o herein by reference.
In providing the antibodies of the present invention to a recipient mammal, preferably a human, the dosage of administered antibodies will vary depending upon such factors as the mammal's age, weight, height, sex, general medical condition, previous medical history and the like.
In general, it is desirable to provide the recipient with a dosage of antibodies which is in the range of from about 5 mg/kg to about 20 mg/kg body weight of the mammal, although a lower or higher dose may be administered. In general, the antibodies will be administered intravenously (IV) or intramuscularly (IM).
Intravenous immunoglobulin (IVIG) can generally be given with a loadin dose of 200 m k g g/ g, with monthly injections of 100 mg/kg. High-dose IVIG may be given at 400-800 mg/kg, for antibody-deficient patients. See, e.g., The Merck Manual of Diagnosis and Therapy, 16th Edition, (Berkow R and Fletcher AJ, Eds.), Merck Research Laboratories, Rahway, NJ (1992).
The peptides and/or antibodies of the present invention are intended to be provided to the recipient subject in an amount sufficient to prevent, or attenuate the severity, extent or duration of the deleterious effects of staphylococcal and streptococcal pyrogenic exotoxins.
The administration of the agents including peptide and antibody compositions of the invention may be for either "prophylactic" or "therapeutic" purpose. When provided prophylactically, the agents are provided in advance of any symptom. The prophylactic administration of the agent serves to prevent or ameliorate any o subsequent deleterious effects of staphylococcal and streptococcal pyrogenic exotoxins. When provided therapeutically, the agent is provided at (or shortly after) the onset of a symptom of infection with bacteria expressing staphylococcal or streptococcal pyrogenic exotoxins. The a ent of the g present invention may, thus, be provided either prior to the anticipated exposure to bacteria expressing staphylococcal or streptococcal pyrogenic exotoxin (so as to attenuate the anticipated severity, duration or extent of disease symptoms) or after the initiation of the infection. The agent may also be provided to individuals at high risk for getting an infection with bacteria expressing staphylococcal or streptococcal pyrogenic exotoxins.
Also envisioned are therapies based upon vectors, such as viral vectors containing nucleic acid sequences coding for the peptides described herein. These molecules, developed so that they do not provoke a pathological effect, will stimulate the immune system to respond to the peptides.
For all therapeutic, prophylactic and diagnostic uses, the peptide of the invention, alone or linked to a carrier, as well as antibodies and other necessary reagents and appropriate devices and accessories may be provided in kit form so as to be readily available and easily used.
Where immunoassays are involved, such kits may contain a solid support, such as a membrane (e. g., nitrocellulose), a bead, sphere, test tube, rod, and so forth, to which a receptor such as an antibody specific for the target molecule will bind. Such kits can also include a second receptor, such as a labelled antibody.
o Such kits can be used for sandwich assays to detect toxins. Kits for competitive assays are also envisioned.
The following examples illustrate certain embodiments of the present invention, but should not be construed as limiting its scope in any way. Certain modifications and variations will be apparent to those skilled in the art from the teachings of the foregoing disclosure and the following examples, and these are intended to be encompassed by the spirit and scope of the invention.
Peptides whose sequences are based on the two highly conserved regions of the staphylococcal and streptococcal pyrogenic exotoxins described herein were constructed. The sequences were based on alignments of the streptococcal pyrogenic exotoxins with the staphylococcal enterotoxins, and the amino acids used in positions with possible degeneracy were the amino acids most frequently found in these positions. Three of the peptides were then catenated and polymerized to produce peptides of greater than 8000 daltons (i.e., peptides 6343, 6345 and 6348, described below). As described further below, peptide 6348 was used to immunize rabbits, which produced high titer antibodies to this peptide. These antibodies were tested for the ability to recognize the streptococcal and staphylococcal pyrogenic exotoxins. Immunological assays (immunoblots) revealed that these antibodies recognized regions common to all the pyrogenic exotoxins. These antibodies were also tested for the ability to neutralize in vitro and o in vivo biological activity of the pyrogenic exotoxins.
These antibodies protected against the biological T-cell proliferation of these toxins in an in vitro blastogenesis assay using human mononuclear cell populations. The lethal effects of staphylococcal toxin SEB and streptococcal pyrogenic toxin SPEA in vivo were also completely blocked by mixing the antibodies with the toxin prior to injection.
Materials and Methods Construction of Synthetic Peptides:
Peptides were constructed by solid phase synthesis (20) using the modifications described by Houghton (10).
1 . GCG Consensus #1 YGGXITXzX3X4XSN ( SEQ ID NO : 1 ) Peptide #1 CMYGGVTEHEGN (SEQ ID N0:3) 2. GCG Consensus #2 KXsX~XeX9XlaX11X1zX13DX14X1sX1sRXmXls LXlgX2pX21X22X23X24Y ( SEQ ID NO : 2 ) peptide #2 KKNVTVQELDYKIRKYLVDNKKLY (SEQ
ID N0:4) As is evident above, synthetic peptides #1 and #2 are not native peptides, i.e., their sequences differ from those found in native toxins. Variations of these peptides have also been constructed in order to generate concatenated polymers of the peptides. These polymers were constructed by the addition of glycine and of additional cysteine residues to the amino- and/or carboxyl- termini of the initial 2 peptides, thus facilitating concatenation via disulfide bond formation (37, 38, 39) . The polvmerizec~ mn1 Pr."1 A~ ,.,o,.,o +-~,o., dialyzed to remove molecules with molecular weights less than 6000-8000 daltons. One polymeric construct is o composed of the monomer: CMYGGVTEHEGNGC (SEQ ID N0:5).
An additional polymer is composed of the peptide:
CGKKNVTVQELDYKIRKYLVDNKKLYGC (SEQ ID N0:6).
In the native toxin molecules, consensus region #1 precedes consensus region #2 by 27 amino acid residues (e.g. [consensus region 1] x27 [consensus region 2]).
We have constructed the peptide:
CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLY (SEQ ID N0:7).
Like the native toxin molecule, this peptide is representative of the two consensus regions joined together in the proper order (region 1 in the N terminal half, and region 2 in the C-terminal half of the molecule), however they are not separated by an additional 27 residues as they are in the native toxins.
We have also constructed concatenated polymers based on the monomer: CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC (SEQ
ID N0:8) .
ID Peptide 6343 CMYGGVTEHEGN (SEQ ID N0:3) 6344 CMYGGVTEHEGNGC* (SEQ ID N0:5) 6345 KKNVTVQELDYKIRKYLVDNKKLY (SEQ ID N0:4) 6346 CGKKNVTVQELDYKIRKYLVDNKKLYGC* (SEQ ID N0:6) 6347 CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLY (SEQ ID
N0:7) 6348 CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC* (SEQ ID
N0:8) Peptides with an (*) are cross-linked polymers composed of the described sequence. It is expected that monomers of these peptides will also be useful in the present invention.
o Generation of anti-peptide sera.
New Zealand White rabbits were immunized by subcutaneous injection with 500 ~.g of peptide in complete Freund's adjuvant. Additional booster injections of 500 ~,g in incomplete adjuvant was administered 4 weeks after the primary injections. Ten days after booster injections, the rabbits were bled, and the anti-peptide titers were determined by ELISA.
Staphylococcal enterotoxins, TSST-1, and j0 streptococcal pyrogenic exotoxins were purchased from Toxin Technology Inc. (Sarasota, FL).
Immunoblots Each of the staphylococcal and streptococcal pyrogenic exotoxins were electrophoresed through 10°s SDS
15 pAGE gels (16) and transferred to nitrocellulose for western blots (33). The western blots were developed using the rabbit anti-peptide 6348 serum (anti-pep 6348 or AP6348) diluted 1:5000, followed by goat anti-rabbit 20 (IgG) alkaline phosphate conjugate (Sigma).
Inhibition of blastoaenesis Human peripheral blood mononuclear cell (PBMC) preparations were stimulated by each of the staphylococcal enterotoxins and streptococcal pyrogenic exotoxins. 100 ng of toxin was used to stimulate PBMC
preparations at cell concentrations of 105 cells per well in 96 well microliter plates. Phytohemagglutinin (PHA) was used in place of the toxins as a positive mitogenic control. Cell culture medium was supplemented with either 10% normal rabbit serum (NRS) or AP6348 serum. Blastogenesis was assayed by incorporation of tritiated thymidine after 5 days of culture (22). All experiments were performed in triplicate.
o Passive protection of rabbits Female New Zealand White rabbits >lyr old were obtained from Hazelton Dutchland Labs, Inc. (Denver, PA). Rabbits were challenged with staphylococcal or streptococcal toxins at doses ranging from 50 to 100 ~Cg/kg, as previously described (24). Briefly, pyrogenic toxins were incubated with either 200 ul of normal rabbit serum or 200 ul of anti-pep #6348 serum for one hour prior to challenge. Toxin-serum mixtures were administered intravenously through the marginal ear veins. Normal control rabbits were treated in an identical manner, with isotonic saline substituted for the pyrogenic toxin. Four hours later, rabbits were given a sub-lethal dose (5 ~g/kg) of endotoxin (E. coli LPS, List Biological Laboratories, Inc., Campbell, CA).
Rabbits were monitored 72 h for clinical signs of toxic shock. These included elevated temperature, diarrhea, cardiopulmonary distress, and conjunctival injection.
Rabbits with severe toxic shock exhibiting cyanosis and temperatures less than 97°F were declared moribund.
Moribund rabbits were euthanized by administration of 5 ml pentobarbital sodium. All animal protocols were reviewed by the Laboratory Animal Research Center at the Rockefeller University.
Results ELISA assays As seen in Figure 4, rabbits raised significant antibody titers to peptide 6348. Similarly, rabbits receiving immunizations with peptides 6344 and 6346 also developed high titers.
wo oonos9s Pc r~s99n2~so -4$-o Recognition of staphylococcal and streptococcal toxins by anti-pep 6348 serum Western blots of the staphylococcal and streptococcal toxins were developed with anti-peptide 6348 serum followed by an anti-rabbit IgG alkaline phosphatase conjugate (Sigma). The results of the western blot shown in Figure 5 indicate the anti-peptide 6348 serum recognizes the conserved regions of the bacterial toxin molecules; SEA, SEB, SED, SEE, SPEA, and TSST-1. SEC did not show a significant reaction with anti-peptide 6348.
Blastogenesis inhibition The percentage of inhibition, of toxin mediated blastogenesis, by AP6348 was assayed. Tritiated thymidine incorporation by human PBMC stimulated with staphylococcal and streptococcal pyrogenic toxins was significantly inhibited by the addition of AP6348 compared to normal rabbit serum (NRS) (Figure 6). This suggests blastogenesis of PBMC in response to the toxins was inhibited by AP6348. The AP6348 serum did not affect the blastogenesis of human PBMC in response to PHA, suggesting a specific inhibition of toxin biologic activity.
In vivo protection of rabbits We tested the ability of AP6348 serum to prevent severe toxic shock in rabbits challenged with SEB and NRS. Rabbits challenged intravenously with a mixture of SEB and NRS developed symptoms of severe toxic shock (Table 2). One rabbit receiving 50 ~g/kg SEB with NRS, and two receiving 100 ~g/kg of SEB with NRS, developed severe toxic shock and were declared moribund within 30 o hrs. In contrast, two rabbits challenged with 50 ~g/kg and 100 ~g/kg SEB with AP6348 developed fever, but this returned to normal by 32 hours. No diarrhea or cardiopulmonary depression was observed. Rabbits were followed for a total of 5 days (data not shown) and appeared fully recovered.
Table 2 Passive Protection of Rabbits Challenged with SEB SPEA and LPS
Toxin LPS Diarrhea Temperature F
~.g/kg\Serum ~g/kg 0 hr 4 hr 24 hr 32 hr 48 hr SEB
nsf\NRS 5 - 100.4 102 101.4 101.2 NT
50\NRS 5 + 101 104.4 102.8 960 100\NRS S + 102 104.6 103 970 100\NRS 5 + 101 104.5 102.6 970 50\APS 5 - 101.4 103.8 103 102 101 100\APS 5 - 100.4 104.4 103 102 101 IS SPEA
50\NRS 5 + 101 104.2 NTO
100\NRS 5 + 102 104.8 NT0 50\APS 5 102 104 103 102 102 100\APS 5 + 101.6 104.4 104 100 970 ns =control rabbit isotonic saline in place of SEB or given SPEA
NRS=Normal rabbit serum APS=Anti-peptide 6348 serum 0=animals were declaredmoribund NT=not taken Discussion Our results demonstrate that antibodies rabbit antiserum generated to peptides representative of two regions with highly conserved amino acid sequences (AP6348) are capable of recognizing most of the staphylococcal enter otoxins and streptococcal pyrogenic exotoxins (e. g. SEA, SEB, SEC, SEE, SPEA, SPEC), as well as TSST-1, using Wes tern blots. We expect that other , o more sensitive assays, will result in the demonstration of binding of these antibodies to additional members, probably all members, of the staphylococcal and streptococcal pyrogenic toxin family.
Since recognition of the toxins by AP6348 was successful, we tested this serum for the ability to inhibit the biological effects of these pyrogenic toxins. AP6348 was capable of inhibiting in vitro blastogenesis of human PBMCs by many of the pyrogenic toxins (e. g., SEA, SEB, SEC, SEE, SPEA, and SPEC).
AP6348 was also able to provide passive in vivo protection of animals challenged with lethal doses of SEB and SPEA. These animals developed fever, however the fever returned to normal within 30 hours and remained normal. Rabbits appeared to be fully recovered within days of challenge.
In contrast, rabbits receiving similar doses of SEB
and SPEA pre-incubated with NRS developed severe toxic shock as evidenced by high fevers, diarrhea, and cardiopulmonary distress. The illness progressed and these animals were declared moribund.
The therapeutic and biological implications of these observations are as follows: (i) antibodies prepared against this peptide may be administered during the early stages of toxic shock or septic shock irrespective of the toxin causing the symptoms and (ii) the peptide may be used as an immunogen to block the toxic effects of this family of superantigens.
PBMCs were isolated via Ficoll-Hypaque Solution.
The appropriate concentration of nonpolymeric peptide o and 2x105 cells in 200~,L of RPMI solution was plated in each well. The cells were incubated for one hour at 37 degrees Centigrade, with mild agitation every 15 minutes. After one hour, 2~,g of SEB was added in each well. The PBMCs were incubated for 72 hours and the results were measured via tritiated thymidine incorporation. The cells were collected and read on a beta counter. The results are shown in Figure 7. Note the dose-response inhibition of blastogenesis demonstrated in Figure 7A. Peptide 6343 (i.e., CMYGGVTEGEGN, SEQ ID N0:3)(Figure 7A) showed more inhibitory activity of SEB than peptide 6346 (i.e., CGKKNVTVQELDYKIRKYLVDNKKLYGC, SEQ ID N0:6) (Figure 7B) or peptide 6348 (i.e., CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC, SEQ ID N0:8) (Figure 7C).
PBMCs were isolated via Ficoll-Hypaque Solution.
150~.g of nonpolymeric 6343 peptide (i.e., CMYGGVTEGEGN, SEQ ID N0:3) and 2x105 cells in 200~L of RPMI solution was plated in each well. The cells were incubated for one hour at 37 degrees Centigrade, with mild agitation zs every 15 minutes. After one hour, 2~.g of either SEB, SEC, SED, SPEC, SPEA, or TSST-1 was added to each well.
The PBMCs were incubated for 72 hours and the results were measured via tritiated thymidine incorporation.
The cells were collected and read on a beta counter.
All experiments were run in triplicate. The results are shown in Figure 8. Note that peptide 6343 inhibited blastogenesis of PBMCs by all of the superantigens tested.
Two-hit Septic Shock Model Based on the two hit septic shock hypothesis described in the background and Figure 2 we have created a model of septic shock. While the amounts of either SPEA or SEB superantigens used in the rabbit model were relatively high, the amounts used in the mouse model were much lower due to D-galactosamine priming, size of animals and synergy between the toxins and LPS. BALB/c mice challenged intra-peritoneally after priming with D-galactosamine (20 mg/mouse) concurrently with LPS
followed by SEB, showed that extremely small amounts of LPS and SEB were needed to effect lethality (46). The synergy between these two mediators of shock was extremely impressive and extended for at least an 18 hour period. We chose an 8 hour delay between the two toxins for our model. We established and optimized doses of toxin for SEA, SEB, SPEA, SPEC, and TSST-1 that would lead to 100% lethality. The doses of the various toxins are shown in Table 3.
Table 3: Doses of various toxins and LPS/ D-galactosamine used in the lethal two hit septic shock model Toxin (~.g) SEA SPEA SEB SPEC TSST-1 2.5 2.0 0.02 2.5 2.0 LPS (~.~g) 0.001 0.001 0.001 0.001 0.001 D-galactosamine 20 20 20 20 20 mg (mg) All mice were sensitized with 0,001 ~,g Lipopolysaccharide (LPS) and 20 mg of D-Galactosamine via intraperioneal injection. The results are shown in Table 4. After six hours, saline or 1.5 mg of the non of meric p y peptide 6343 was administered to the experimental mice by subcutaneous injection. One hour later, the mice were injected again with either saline or 1.5 mg peptide (3.0 mg total). One hour later, all mice were challenged with 0.02 ~.g SEB, SPEA or TSST-1 (via intraperitoneal injection) and the mice were observed overnight. In this model, it has been observed that peptide 6343, given one and two hours before administration of the toxic dose of the indicated toxin, protected 5 out of 6 mice exposed to toxin SEB; 2 out of 2 mice exposed to the toxin SPEA and 2 out of two mice exposed to the toxin TSST-1.
Table 4 The Use of Peptide 6343 to Block the Superantigen Induction of Septic Shock in a Mouse Model Mice Dose A me Tota A me Tota A me Tota and AdministrationSEB SPEA TSST-1 ControlSaltine 0 6 (0% 0 2 0%) 0 2 (0%) subcutaneous injection Peptide 5 6 83%) 2 2 (100% 2 2 (100%
3.0 mg subcutaneous injection Two streptococcal antigens SEG and SEH have recently been synthesized and three new streptococcal exotoxins, i.e., SPEG, SPEH and SPEZ, have recently been described by Dr. Fraser and colleagues (61).
In order to determine whether 6343 peptide is capable of inhibiting the toxic effects of the streptococcal exotoxins SPEG, SPEH and SPEZ, experiments similar to those described above were conducted.
In a similar manner as above, PBMCs were isolated via Ficoll-Hypaque Solution. Either O~g, 75~Cg, 100~g or 150~,g of nonpolymeric 6343 peptide (i.e., CMYGGVTEGEGN, SEQ ID N0:3) and 2x205 cells in 200~L of RPMI solution IS was plated in each well. The cells were incubated for one hour at 37 degrees Centigrade, with mild agitation every 15 minutes. After one hour, 2~.g of the recombinant forms of either SPEG, SPEH, SPEZ, which were provided by Dr. Fraser of the University of Auckland, New Zealand, was added to each well. The PBMCs were incubated for 72 hours and the results were measured via tritiated thymidine incorporation. The cells were collected and read on a beta counter. The results are shown in Figure 9. Note that peptide 6343 inhibited blastogenesis of PBMCs by the bacterial superantigens SPEG, SPEH and SPEZ.
To determine the nature of the inhibition of superantigen stimulation by the inhibitory peptides and antigens, purified MHC class II molecules (obtained from J. Strominger, Harvard University) were used in a competitive ELISA to determine binding to this molecule.
o The purified MHC were immobilized on a 95 well plate and 4~.g SEB was added. After appropriate washing a rabbit monoclonal anti-SEB antibody (Toxin Tech) was added to the ELISA followed by a colorimetric reagent. The plate was read on an ELISA plate reader. In similar experiments various concentrations of the peptide 6343 ( i . a . , O~g, 50~.g, 75~.g, 100~,g, or 150~Cg) were added to the immobilized MHC before the addition of SEB. Binding of the peptide to the MHC was demonstrated by the decreased amount of SEB bound to the MHC, as indicated by a decreased amount of antibody binding measured by a decreased color reaction. The results are shown in Figure 11. The results indicate that peptide 6343 binds very strongly to the MHC complex and is able to compete effectively for the site of SEB binding.
The binding of the peptide to the MHC complex of monocytes was supported by con-focal microscopy results of experiments using a method described in Ojcius et al.
(68) using fluorinated peptides constructed for us by NEN LifeSciences, antibodies directed against MHC class II proteins, and a phycoerythrin (PE) antibody directed to the anti-MHC antibody. Various doses of the peptide and various concentrations of the cells were titrated to achieve optimum binding of the peptide. Confocal microscope pictures (Figure 11) show similar patterns of binding for anti-MHC antibodies and for the flourinated peptides, supporting the determination that the peptide binds to the cells' MHC complex.
° DISCUSSION
In the experiments described herein, the dose of peptide 6343 used directly for the prevention of toxic and septic shock was 3 mgs per mouse. Thus, the direct use of peptides in septic or toxic shock would be expected to involve doses in the range of several grams for the acute treatment of shock in humans. Peptide 6343, possibly because of its small size, does not induce detectable antibodies in rabbits and mice, yet it still has all the therapeutic properties described for the anti-peptide antibodies. Therefore, if desired, it is expected that the peptide can be used repeatedly in the same individual without raising anti-peptide antibodies.
o References 1. Bergdoll, M. S. 1985. The staphylococcal enterotoxins-an update., 247-254. In J.
Jeljaszewicz (ed.). The staphylococci. Gustav Fischer Verlag, New York, NY.
2. Blomster-Hautamaa, D. A., B. N.
Kreiswirth, J. S. Kornblum, R. P. Novick and P. M.
Schlievert. 1986. The nucleotide and partial amino acid sequence of toxic shock syndrome toxin-1. Journal of Biological Chemistry. 261:15783-15786.
3. Choi, Y., B. Kotzin, L. Herron, J.
Callahan, P. Marrack and J. Kappler. 1989. Interaction of staphylococcus aureus toxin superantigens with human IS T cells. Proc. Natl. Acad. Sci. USA. 86:8941.
4. Fleischer, B. and H. Schrezenmeier.
1988. T cell stimulation by staphylococcal enterotoxins. Clonally variable response and requirement for major histocompatibility complex class II molecules on accessory or target cells. Journal of Experimental Medicine. 167:1697.
5. Goshorn, S. C. and P. M. Schlievert.
1988. Nucleotide sequence of streptococcal exotoxin tYPe C. Infect. Immun. 56:2518-2520.
6. Grossman, D., M. Van, J. A. Mollick, S.
K. Highlander and R. R. Rich. 1991. Mutation of the disulfide loop in staphylococcal enterotoxin A.
Consequences for T cell recognition. J. Immunol.
147:3274-3281.
OF TOXIC SHOCK SYNDROME AND SEPTIC SHOCK
RELATED APPLICATIONS
This is a continuation-in-part application of co-pending U.S. Application Serial No. 09/168,303 filed October 7, 1998, which is in turn a continuation-in-part of co-pending U.S. Application Serial No. 08/838,413 filed April 7, 1997. Pursuant to 35 USC 365, U.S.
APPlication Serial No. 09/168,303 is also a continuation-in-part of co-pending International Application PCT/US98/06663, filed April 1, 1998. The entire disclosure of U.S. Application Serial No.
08/838,413 and U.S. Application Serial No. 09/168,303 are incorporated herein by reference.
FIELD OF THE INVENTION
This invention relates to compositions and methods for protecting against, or reducing the severity, of toxic shock syndrome and septic shock from bacterial infections. More particularly it relates to peptides, which may be polymeric, and carrier-conjugates thereof, derived from homologous sequences of the family of staphylococcal and streptococcal pyrogenic toxins. The peptides of the invention are useful to prevent, treat, or protect against the toxic effects of bacterial toxins, including most, if not all, of the staphylococcal and streptococcal pyrogenic toxins.
These are also useful to induce serum antibodies and may also be useful in diagnostic assays.
The invention also relates to antibodies induced by the peptides and/or carrier-conjugates and their use to o prevent, treat, or protect against the toxic effects of bacterial toxins, including most, if not all, of the staphylococcal and streptococcal pyrogenic toxins.
The invention also relates to compositions and methods to protect against, or ameliorate the effects of, autoimmune diseases which are associated with, or are the result of, the presence of staphylococcal or streptococcal toxins.
The invention also relates to diagnostic assays and kits to detect the presence of staphylococcal and streptococcal pyrogenic toxins, or antibodies thereto.
The invention also relates to isolated and purified nucleic acids encoding the peptides of the invention and transformed host cells containing those nucleic acids.
BACKGROUND OF THE INVENTION
The pyrogenic exotoxins of Group A streptococci and the enterotoxins of Staphylococcus aureus, which are also pyrogenic exotoxins, constitute a family of structurally related toxins which share similar biological activities (I1, 13). The staphylococcal and streptococcal pyrogenic exotoxins also share significant amino acid homology throughout their sequences (11, 19, 40). This pyrogenic exotoxin family contains nine main toxin types, and several allelic variants (subtypes) have been described. Several studies have shown that the toxins share common motifs based on immunologic cross reactivity between the toxins (26, 27). They stimulate CD4+, CD8+ and y8+ T cells by a unique mechanism. These toxins share the ability to bind the ~3 chain variable region (Vp) elements on the lateral face of the T cell receptor (TCR) and simultaneously bind to o the lateral face of the class II major histocompatibility complex (MHC) of antigen presenting cells (Figure 1), causing an aberrant proliferation of specific T-cell subsets (3, 4, 12). This property of the toxins has labeled them as "superantigens" (36) since they do not interact with the MHC and TCR
molecules in the manner of conventional antigens (14, 18) and produce a massive proliferation of T cells.
The variability of the sequences in the TCR-binding region and within the MHC-II-binding regions most likely provides the different superantigen toxins their specificities for different Va molecules and variable affinities for MHC-II types.(69-70) The cross-linking of TCR with MHC-II molecules by superantigens causes a profound blastogenesis of lymphocytes and antigen-presenting cells. The resulting stimulation of leukocytes leads to a significant increase in cytokine production.
Monocytes stimulated with bacterial superantigens produce the Thl cytokines IL-2 and IFN-y and the anti-inflammatory cytokines IL-10 and IL-1 receptor antagonist. (71). T cells activated by superantigen stimulation produce IL-12. (72). Whole preparations of peripheral blood mononuclear cells containing lymphocytes and antigen-presenting cells elicited a wide range of inflammatory cytokines in significant amounts.
The generation of monocyte cytokines such as IL-1, IL-6, TNF-a, and TNF-(3 was dependent on the presence of T
cells. (73).
Costimulatory molecules important in conventional immune responses also play a significant role in the o response of immune cells to superantigens. The costimulatory T cell antigen, CD28, and its corresponding ligand on MHC-II-bearing cells, B7, contribute to superantigen mitogenicity. (74, 75).
Other costimulatory molecules, such as LFA-1/ICAM-1 and VLA-4/VCAM-1, also contribute to the activation of immune cells by superantigens.(76, 77). These immunostimulatory activities of superantigens are crucial to their ability to cause injury to the host.
The bacterial toxins cause a variety of syndromes in humans. Staphylococcal enterotoxins have been implicated in staphylococcal food poisoning (26), as well as toxic shock like syndromes (1). The gene sequences and deduced amino acid sequences of at least six staphylococcal enterotoxins ("SE"): A, B, C, D, E
and H, are known, i.e., SEA, SEB, SEC, SED, SEE, and SEH
(19, 23). The streptococcal pyrogenic exotoxins ("SPE") have been implicated in causing the symptoms of scarlet fever and toxic shock like syndrome (8, 20, 30). The sequences of three members of this family are known:
SPEA, SPEC, and SSA (5, 23, 35).
Toxic shock syndrome toxin (TSST-1) from S. aureus shares similar biological activity with the SE's and SPE's, however amino acid sequences of this toxin are significantly different from these two classes of toxins (2). Structural analysis suggests that, despite the differences in amino acid composition, the overall topology of TSST-1 and the SE/SPE family of toxins is similar (41). The molecular structure of SE's and SPE's has been determined by various methods. Reviews concerning the molecular structures are available (19, 62). Molecular evolution studies of the SE/SPE family of a toxins suggests that the toxins can be grouped into two main clades(34). All these toxins are highly resistant to denaturation by heat and to proteases. With the exception of TSST-1, they are soluble proteins of approximately 230 amino acids and have a central disulphide loop. In contrast TSST-1 has only 194 amino acids and does not possess any cysteines.
It has been suggested that the conservation of amino acids is important to maintaining the structure necessary for the biological activity of the toxins (32). Mutations constructed in various positions throughout the SPEA and SEB molecules were sufficient to inactivate biological activity (6, 15). Mutations at various points throughout the molecules often had different effects, su ggesting that functional activities could not be attributed to any one region of the toxins (7). These results suggested that a functional tertiary structure must be maintained. Chemical modifications of highly conserved histidine residues inactivated biologic activity (29). The high conservation of the disulfide loop in the SE's and SPE's suggests an important role in the structure of the SE/SPE family of toxins. Studies show the disulfide loop is required for mitogenic activity of SEA and SEB. Reduction of the disulfide loop inactivated T cell stimulatory activity, but did not affect MHC-II binding and stimulation of monocytes (54). Peptide cleavages within the loop had no effect on T-cell mitogenicity, however cleavage of conserved sequences outside the loop of SEA resulted in loss of mitogenic activity. The loop and conserved adjacent sequences appear to be associated with avidity of the toxins to the TCR, and do not contribute to the specificity of toxins for a particular Vp type (6) .
Residues determining TCR V~ specificity appear to be located within the carboxy-terminus of the SE/SPE toxins (59), while residues critical for MHC-II binding appear to be located in the amino-terminal region, and the central portion of the molecule near the disulfide loop (53). The disulfide loop and adjacent highly conserved sequences contribute to the structural integrity of the toxins, and serve to bring the TCR and MHC binding regions in functional proximity to each other (65).
The SEs are named for their ability to induce gastrointestinal illnesses upon oral intake of a few micrograms of the toxin. The clinical effect appears in IS 2 to 4 hours and is manifested by nausea and diarrhea.
These symptoms appear to be caused by leukotrienes and histamine released from mast cells. Additionally, both the staphylococcal and streptococcal exotoxins are implicated in gram-positive shock. Although superantigen-related septic shock appears to be primarily mediated by tumor necrosis factor (TNF)-a and interleukin (IL) 12, the contribution of other cytokines cannot be discounted. (78, 79, 80).
The physiologic response to superantigens is similar to septic shock induced by gram-negative endotoxin (lipopolysaccharide, LPS). In fact, LPS and superantigens can work synergistically to produce lethal toxic shock. (81, 82, 83). Toxic shock syndrome can be exacerbated by the synergistic effects of TSST-1 with the SE/SPE family of toxins. (84, 85). Superantigen stimulation of immune cells can exacerbate autoimmune syndromes by causing the expansion of autoreactive T
o cell subsets, upregulation of MHC-II expression, and the potentiation of cytotoxic T cell response (86, 87, 88, 89, 90, 91).
Toxic shock syndrome is a specific syndrome caused by either the Stapylococcal or Streptococcal organisms.
It is specifically caused by the toxins produced by these bacteria. Clinically it often occurs in young women and children and is characterized by a raised temperature, low blood pressure, a rash that eventually leads to skin loss especially on the palms and the soles and multi-organ involvement.
Septic Shock on the other hand involves both gram negative as well as gram positive organisms, occurs in all groups of patients especially the elderly and post-surgical. It has similar symptoms except for the lack of a skin losing rash. Both diseases have a high mortality-however there are many more cases of septic shock as compared to toxic shock. The term "septic shock" is used herein to describe hypotension and organ failure associated with bacterial infections.
"Toxic shock like syndrome" is the term previously used to describe the syndromes caused by staphyloccal and streptococcal pyrogenic bacterial exotoxins other than toxic shock syndrome toxin (TSST-1) from S. aureus.
Currently, the term "toxic shock syndrome" is used to describe the syndromes caused by TSST-1 and the other pyrogenic exotoxins, and is the terminology used hereinafter.
Toxic shock syndrome can be exacerbated by the synergistic effects of TSST-1 with the enterotoxin/pyrogenic toxin family of toxins (9, 25).
Gram negative bacterial endotoxin and the pyrogenic _g_ o toxins can work synergistically to produce intractable shock ( 17 , 3 0 ) .
With respect to septic shock, lipopolysaccharide (LPS) is an integral part of the cell wall of Gram-negative bacteria and is a potent inducer of cytokine release by macrophages (52). During the induction phase of septicemia, LPS binds to the CD14 receptors of macrophages and triggers the release of a number of cytokines including Interleukin-1 (IL-1), and Tumor Necrosis Factor-a(TNF- a) (49). Accordingly, therapeutic strategies for septic shock have centered on the neutralization of LPS or LPS-induced cytokines (64).
Unfortunately, trials using either monoclonal antibodies directed against part of the LPS molecule or the use of CD14 soluble receptors have riot been very promising (45). The reasons for these failures might be: 1. The type of patient selected (many were already in irreversible shock). 2. The monoclonal antibody did not block all sites of LPS. 3. Soluble CD14 receptors did not block all LPS molecules.
Toxic shock syndrome and septic shock are still among the most life threatening syndromes affecting humans. It is estimated that approximately 20,000 cases of toxic shock syndrome occur each year of with a 10%
mortality rate (66). With respect to septic shock approximately 400,000-500,000 cases occur each year with a 50% mortality (63). Present therapy is primarily symptomatic with administration of fluids, antibiotics, pressor agents and occasionally steroids (56). There is no vaccine available for toxic shock syndrome since all of the superantigens are antigenically distinct even though there is some sequence homology present in all WO 00!10598 PCT/US99/22180 o the superantigens. There have been numerous vaccine trials for septic shock none of which have been successful.
With respect to the failed vaccine trials for septic shock, we believe that there was a failure to recognize that the interaction between the superantigens described above and LPS enhances the lethal potency of both these antigens by about 1000 fold. In contrast, each antigen when given alone requires a much higher dose for lethal septic shock (46).
Hence, it is proposed that at least two independent pathways of lethal septic shock can occur. LPS and peptidoglycan interact with macrophages. The superantigens interact with T cells. In both cases target cells are induced to release large amounts of cytokines. There is increasing evidence that gram-positive infections frequently accompany gram-negative infections in patients with septic shock (see article by Range!-Frausto, pages 299-312) (96). Exposure to gram-negative endotoxin produces a state of macrophage hyperesponsiveness on subsequent stimulation (92). A
similar state is seen with monocytes in septic shock.
Our group and others have shown that LPS and superantigens can act synergistically to produce lethal septic shock in animal models (93). It is our hypothesis that a significant amount of septic shock involves an early gram-negative infection that causes significant symptoms of vasodilation and hypotension.
This is then treated with fluids and antibiotics, leading to early recovery by the patient. Some days later, a gram-positive insult either via a line sepsis °f the skin or gastrointestinal flora may cause severe o irreversible shock in a previously LPS-sensitized patient. This model is depicted graphically in Figure 2 herein (94).
In other words, since Gram-negative and Gram-positive organisms can be recovered from patients with sepsis, it appears that it is the "two hit" hypothesis that is operative and the interaction between LPS and the superantigens markedly enhances the lethal properties of both molecules. In this model, the interruption of the toxin pathway by anti-peptide antibody(ies) or by peptides) of the invention prevents the onset of lethal shock induced by the combination of the LPS and one or more of the superantigens.
SUMMARY OF THE INVENTION
The present invention relates to the identification of consensus sequences derived from two conserved regions of the staphylococcal enterotoxins and streptococcal pyrogenic toxins (hereinafter called "region 1" and "region 2") and the discovery that compositions comprising amino acid sequences based on these two conserved regions of the staphylococcal enterotoxins and streptococcal pyrogenic exotoxins are capable of inducing antibodies which react with a variety of staphylococcal and streptococcal pyrogenic exotoxins and are also capable of ameliorating or preventing diseases related to the deleterious effects of these toxins.
The invention also relates to compositions and methods for preventing and treating diseases related to the release of certain pyrogenic exotoxins from bacteria.
o This invention provides peptides comprising amino acid sequences which reduce, inhibit or eliminate the deleterious effects of bacterial toxins and/or axe capable of inducing antibodies that reduce, inhibit or eliminate the deleterious effects of bacterial toxins, S such as those of staphylococcus and a variety of streptococci. Antibodies may be induced by administration of a pharmaceutical composition and/or vaccine containing a composition comprising a peptide 1~ derived from one or both of the two conserved regions described herein, or a structurally and/or immunologically related antigen.
The amino acid sequences provided by this invention are sufficiently common to all members of this family of pyrogenic exotoxins to be useful for eliciting antibodies which are cross-reactive with toxins derived from various bacteria.
The amino acid sequences provided by this invention 20 are also useful for new methods of preventing and treating symptoms associated with the bacterial release of the staphylococcal enterotoxins and the streptococcal pyrogenic exotoxins. Such methods include, for example, administering to an individual who is suspected of having an infection or developing and/or having a toxic or septic reaction, a compound comprising at least one of the consensus amino acid sequences of this invention in an amount sufficient to inhibit superantigen 30 stimulation of T-cells, preferably an amount sufficient to reduce, inhibit or eliminate the deleterious effects of the exotoxins. Such methods also include administering to an individual at risk of infection or 3S developing a toxic reaction to the exotoxins at least 0 one of the consensus amino acid sequences of this invention in an amount sufficient to elicit the production of antibodies to the exotoxins.
In a preferred embodiment of this invention, an individual at risk for developing toxic or septic shock syndrome or an individual with symptoms of toxic shock syndrome or septic shock may be treated by administering to such individual a composition comprising at least one of the peptides of this invention and/or carrier-conjugate thereof.
In another preferred embodiment of this invention, an individual at risk for developing toxic shock syndrome or septic shock, or an individual with symptoms of toxic shock syndrome or septic shock, may be treated by administering to such individual antibodies which have been generated in a mammal immunized with at least one of the compositions of this invention.
Vaccines and pharmaceutical compositions comprising at least one of the consensus amino acid sequences and a physiologically acceptable carrier and optionally an adjuvant are also part of this invention.
Another object of the invention is to provide antibodies induced by the peptides and carrier-conjugates thereof. These antibodies may be used to prevent, treat, or protect against the toxic effects of most, if not all, of the staphylococcal and streptococcal pyrogenic exotoxins. The antibodies may also be useful to protect against, or ameliorate the effects of, autoimmune diseases which are associated with, or are the result of, the presence of staphylococcal or streptococcal pyrogenic exotoxins.
These antibodies are also useful in diagnostic assays o and kits to detect the presence of staphylococcal and streptococcal pyrogenic exotoxins and to aid in the diagnosis of diseases related to the presence of those toxins.
Another object of the invention is to provide isolated and purified nucleic acids encoding the amino acid sequences of the invention, as well as suitable expression systems, vector components and transformed host cells containing those nucleic acids.
BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1. Schematic diagram of the interaction between a T cell receptor, superantigen, and a class II
MHC molecule. Superantigens bind to common sequences in class II MHC molecules and T cell receptors that lie outside the normal antigen-binding sites. T cell activation by superantigens is not limited by the antigenic specificity of the T cell.
Figure 2. Diagram of the "two hit" model of septic shock.
Figure 3. Comparison of the synthetic peptide sequences to conserved regions 1 and 2 of the staphylococcal enterotoxins (SEA, SEB, SEC, SED, SEE, and SEH), and sire tococcal p pyrogenic exotoxins (SPEA, SPEC, and SSA). Staphylococcal toxic shock syndrome toxin 1 (TSST-1) was compared with the region 2 peptide.
Numbers represent the residue positions as a reference to where these regions exist in the whole toxin molecules. Sequences are from either the Swiss protein or GenBank databases under the following accessian numbers. Swiss protein: SPEA, P08095; SPEC, P13380;
SEA, P13163; SEB, P01552; SEC, P01553; SED, P20723; SEE, o P12993. GenBank: SEH, U11702; SSA, L29565; TSST1, J02615.
Figure 4. ELISA titers of antibodies from rabbits immunized with polymeric peptide #6348. The peptide was diluted so that it was delivered to each well to give a final concentration of 2 ~,g/100 ~1. The serum was then diluted to 1:1,000; 1:10,000; 1:100,000; 1:500,000; and 1:1,000,000 and 100 ~1 of each dilution of serum was placed in each well. Experiments were run in triplicate for each dilution of serum. Note the 1 log higher titers of rabbit #443 serum as compared to rabbit #442 serum. Cut off readings were at O.D. 0.6.
Figure 5. 12% SDS PAGE gel immunoblot of a variety of staphylococcal and streptococcal toxins developed with the anti-peptide 6348 antibody. Note bands of correct molecular weight (M. W.) of each toxin identified by the anti-peptide antibody. Lane 1: SPEA, lane 2:
SEA, lane 3: SEB, lane 4: SED, lane 5: SEE, lane 6: SEC
and lane 7 TssT-1. Note bands at appropriate M.W. in lanes 1-4. Fainter bands are seen in lanes 5 and 7.
Figure 6. Bar graphs of blastogenesis assays of human mononuclear cell populations stimulated by various toxins in the presence of normal rabbit serum and anti-peptide 6348 serum. Note the marked inhibition of SEB, SEC, SEE, SPEA and SPEC by the anti-peptide antibody. Less, but definite, inhibition of SEA by the anti-peptide antibody was also seen.
Fi ure 7. Bar g graphs of blastogenesis assays of human mononuclear cell populations stimulated by SEB in the presence of (A) peptide 6343 (i.e., CMYGGVTEHEGN, SEQ ID N0:3),(B) peptide 6346 (i.e., CGKKNVTVQELDYKIRKYLVDNKKLYGC, SEQ ID NO: 6)) and (C) o peptide 6348 (i.e., CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC, SEQ ID N0:8).
Figure 8. Inhibition of SEB, SEC, SED, SPEC, SPEA
and TSST-1 toxin blastogenesis of peripheral blood mononuclear cells (PBMC) by the 6343 peptide. 2 x 105 PBMC were stimulated with either 2~g of the indicated toxin or a combination of 2~,g of the toxin with 150~.g of the 6343 peptide. These were incubated for 72 hours and the results were measured via tritiated thymidine incorporation. CPM represents counts per minute. Note that the single peptide (6343) inhibited all of the superantigens tested.
Figure 9. Inhibition of SPEG, SPEH, and SPEZ toxin blastogenesis of peripheral blood mononuclear cells (PBMC) by the 6343 peptide. 2 x 105 PBMC were stimulated with either 2~.g of the indicated toxin or a combination of 2~g of the toxin with the indicated amount of the 6343 peptide. These were incubated for 72 hours and the results were measured via tritiated thymidine incorporation. CPM represents counts per minute. Normal represents normal media. Note that the single peptide (6343) inhibited the superantigens SPEG, SPEH and SPEZ.
Figure Z0. (A). Binding of peptide 6343 to the MHC complex as measured by ELISA. (B). Inhibition of binding of SEB toxin biding by peptide 6343 as measured by decreased anti-SEB binding at increased concentrations of added peptide 6343.
Figure 11. Confocal microscope pictures. (A) Binding of peptide 6343 is indicated by the green color.
(B) Binding of anti-MHC peptide is indicated by the red color. (C) Combined picture showing stippled pattern of o red and green color. Binding of peptide 6343 is indicated by the green color and binding of anti-MHC
peptide is indicated by the red color.
DETAILED DESCRIPTION OF THE INVENTION
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only, and are not restrictive of the invention, as claimed. The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate an embodiment of the invention and, together with the description, serve to explain the principles of the invention.
Two consensus patterns, corresponding to conserved region 1 and region 2, respectively, are identified as common to members of the staphylococcal enterotoxin and streptococcal pyrogenic toxin family of toxins when the Program "Motifs" in a software package from the Genetics Computer Group, Inc. ("GCG") is run using the streptococcal SPEC toxin as an example. "Program Manual for the Wisconsin Package, Version 8, September 1994, Genetics Computer Group, 575 Science Drive, Madison, Wisconsin USA 53711"
incorporated herein by reference.
The first consensus sequence ("GCG consensus #1") identified by the Motifs program has the amino acid sequence YGG(LIV)TXXXXN, which is rewritten herein as YGGX1TX2X3X4XSN (SEQ ID NO:1) , wherein X1 is selected from the group consisting of L, I, or V; and X2, X3, X4 and XS
are each independently selected from the group consisting of any amino acid. This pattern is present in the staphylococcal enterotoxins and streptococcal o pyrogenic exotoxins, but not in TSST-1. The sequence begins immediately at the COOH-terminal side of the cysteine loop. The second consensus sequence ("GCG
consensus #2") identified by the Motifs program has the amino acid sequence KXX(LIV)XXXX(LIV)DXXXRXXLXXXXX(LIV)Y, rewritten herein aS KX6X~XgXgX1pX11X12X13DX14X15X16RX17X18LX19X20X21X22X23X24Y (SEQ
ID NO: 2) , wherein X8, X13 and X24 are each independently selected from the group consisting of L, I and V, and X6r X7r X9r XlOr Xllr Xl2r X14 XlSr Xl6r Xl7r XlBr Xl9r X20r Xzl, X22 and X23 are each independently selected from the group consisting of any amino acid. This pattern is present in the staphylococcal enterotoxins, streptococcal pyrogenic exotoxins, and TSST-1.
One object of the invention is to provide compositions comprising peptides comprising amino acid sequences based on these two conserved regions of the staphylococcal enterotoxins and streptococcal pyrogenic toxins. These peptides may be used for eliciting an immunogenic response in mammals, including responses which provide protection against, or reduce the severity, of toxic shock or septic shock from staphylococcal or streptococcal infections. These peptides may also be useful to protect against, or ameliorate the effects of, autoimmune diseases which are associated with, or are the result of, the presence of staphylococcal or streptococcal pyrogenic exotoxins.
These peptides are also useful in diagnostic assays and kits to detect the presence of antibodies to staphylococcal and streptococcal pyrogenic exotoxins and to aid in the diagnosis of diseases related to the Presence of those toxins.
o The peptides of the invention are those derived from either one or both of the following two consensus sequences:
YGGX1TX2X3X4X5N (SEQ ID NO:l) , wherein X1 is selected from the group consisting of L, I, or V; and X2, X3, X4 and XS
are each independently selected from the group consisting of any amino acid.
KXX(LIV)XXXX(LIV)DXXXRXXLXXXXX(LIV)Y, rewritten herein aS KX6X7XgX9X1pX11X12X13DX14X15X16RX17X18LX19X20X21X22X23X24Y ( SEQ
ID NO: 2) , wherein X8, X13 and X24 are each independently selected from the group consisting of L, I and V, and X6i X7i X9i XlOi Xlli Xl2i Xl4i XlSi Xl6i Xl7i XlBi Xl9i X201 X21, X2z and X23 are each independently selected from the group consisting of any amino acid.
A preferred consensus sequence of the invention from Region 1 (consensus #la) has the amino~acid sequence X25X2sYGGXITX2X3X4X5N (SEQ ID NO: 28) , wherein X1 is selected from the group consisting of L, I, and V;
X2. X4 and XS are each independently selected from the group consisting of any amino acid; and X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; but preferably X1 is selected from the group consisting of I
and V; X2 is selected from the group consisting of L, E, K, P and N; X3 is selected from the group consisting of H and A and no amino acid; X4 is selected from the group consisting of D, N, E, Q, and H; XS is selected from the group consisting of N, G, S, and R; X25 is selected from the group consisting of C and Y and no amino acid; and X26 is selected from the group consisting of M, T, L, I, and no amino acid.
A Preferred consensus sequence of the invention o from region 2 (consensus ##2a) has the amino acid sequence : KX6X~XgXgX1pX11X12X13DX14Xi5X16RX17X18X27Xy9X20X21 X22X23X24Y (SEQ ID NO: 29) , wherein Xa, X13 and X24 are each independently selected from the group consisting of L, I
and V; X6, X~, Xs. Xlo~ X11. X12 Xla~ Xis~ Xls. Xl~. Xls Xls.
X2o, X21. X22, and X23 are each independently selected from the group consisting of any amino acid; and X2~ is selected from the group consisting of L and Y; but preferably X6 is selected from the group consisting of K
and D; X, is selected from the group consisting of N, K, S, E, M, I and Q; X$ is selected from the group consisting of L and V; X9 is selected from the group consisting of T and A; Xlo is selected from the group consisting of V, A, L, F and I; X11 is selected from the group consisting of Q and S; X12 is selected from the group consisting of E and T; X13 is selected from group consisting of L and I; X14 is selected from the group consisting of L, Y, I, A, F and C; Xls is selected from the group consisting of Q, L, K and E; X16 is selected from the group consisting of A, T, I and V; X1-, is selected from the group consisting of R, H, N and K; X18 is selected from the group consisting of Y, F, I, L and Q; X19 is selected from the group consisting of Q, V, I, H, S, T and M; X2o is selected from the group consisting of E, K, N, G, D, S and Q; X21 is selected from the group consisting of K, N, D, R and I; X22 is selected from the group consisting of Y, K, L, F and H; X23 is selected from the group consisting of N, K, G and Q; X24 is selected from the group consisting of L and I; and X2~ is L.
The following Table 1 lists the amino acids that are found at each of the variable positions in the o sequences shown in Figure 3, and the number of times they appear at that position:
Table Frequency of the ids in riablepositions amino the va ac in the sectuences shown in gure 3 Fi Xz 3L 2E 1K 2P 1N
X3 7H lA one deletion (no amino acid) Xs 3N 4G 1S 1R
Xs 9K 1D
X~ 3N 1K 1S lE 1M lI 1Q
Xa 9V 1L
X9 9T lA
Xlo 4V 3A 1L 1F lI
X11 9Q 1s X13 9L lI
Xls 3Q 1L 5K lE
Xl~ 2R 3H 1N 4K
X19 2Q 2V lI 1H 1S 2T 1M
X2o lE 2K 1N 1G 3D 1S 1Q
X21 4K 3N 1D 1R lI
Xz4 8L 2I
X2s 8C lY
X2~ 9L lY
o In the peptides of the present invention, X1, Xe, X13 and X24 may each independently be selected from the group consisting of L, I and V; X2, X3, X4, Xs, X6, X7, X9. X10. X11. X12. X14. Xls. X16. X17. Xle X19. X201 X21. X22.
Xz3. Xzs and X26 may each independently be any amino acid;
X3. Xzs and X26 may also each independently be no amino acid; and X27 is selected from the group consisting of L
and Y. However, in general, the amino acids present at the positions X1 to X27 in the toxins shown in Figure 3 (and listed in Table 1) are preferred for those positions, and the amino acids present most often at those positions in the toxins shown in Figure 3 (and listed in Table 1) are more preferred. For example, from Figure 3, and Table 1, it can be determined that H
(histidine) is present in seven toxins at position X3 and A (alanine) is present in one toxin at position X3, and there is no amino acid present in one toxin at X3.
These are the preferred amino acids for position X3.
The more preferred amino acid for position X3 in a peptide of the invention is H (histidine). The more preferred amino acids for X1 through X26 are: X1 =
valine; X2 = leucine; X3 = histidine; X4 = glutamic acid;
Xs = glycine; X6 = lysine; X7 = asparagine; X8 = valine;
Xs = threonine; Xlo = valine; X11 = glutamine; X12 =
glutamic acid; X13 - leucine; X14 = leucine, tyrosine, isoleucine or phenylalanine; X15 = lysine; X16 = alanine;
X17 = lysine; X18 = tyrosine; X19 = glutamine, valine or threonine; X2o = aspartic acid; Xzl = lysine; X22 =
lysine; X23 = lysine; X2g = leucine; X25 = cysteine; X26 =
methionine; and X27 = leucine. But note that in the exemplified peptides of the invention described hereinbelow, i.e., SEQ ID NOS: 6, 7 and 8, inosine (I) o is used at position X16 instead of the more frequently found alanine (A).
As is evident from Figure 3 and the above Table 1, some amino acid residues are much more highly conserved than suggested by the GCG package data provided by the "Motifs" program.
In region 1, the preferred consensus is larger (consensus #la), and usually includes a C in the first position (X25) . The second residue (X26) is most often a M, but this can vary. In the ninth position (X3), H is the most highly conserved. The eleventh residue (XS) is most often a G.
In region 2, the preferred consensus (consensus #2a) is much more highly conserved than suggested by the GCG program, especially if one excludes TSST-1 sequences from consideration, as follows: The second position (X6) is more highly conserved than suggested, being almost exclusively a K; the fourth residue (X8) is always a V followed exclusively by a T in the fifth position (X9); the sixth position (Xlo) is somewhat variable; but the seventh position (X11) is always a Q, followed by E (X12). The next position is almost always an L (X13) , and the second to last position (X24) is almost always an L.
Thus, additional modified consensus sequences for region 1 and region 2, which are of narrower scope than the GCG consensus sequences #1 and #2 and the modified consensus sequences #la and #2a, are as follows:
o Consensus #lb:
CMYGGX1TX2HX4GN (SEQ ID NO: 30) wherein X1 is V or I, preferably V;
X2 is L, E, K, P or N, preferably E or L;
and X4 is D, N, E, Q or H, preferably E.
Consensus #2b:
KKX~VTXIOQELDX14X1sX1sRXI~XleXz~XzsX2oXz1Xa2Xa3LY (SEQ ID
N0:31) wherein X~ is N, K, S, E, M, I or Q, preferably N;
X10 is V, A, L, F or I, preferably V;
X14 is L, Y, I, A, F or C, preferably Y;
Xls is Q, L, K or E, preferably K;
Xls is A, T, I or V, preferably I;
Xl~ is R, H, N or K, preferably K;
X18 is Y, F, I, L or Q, preferably Y;
X19 is Q, V, I, H, S, T or M, preferably V;
X2o is E, K, N, D, G, S or Q, preferably D;
X21 is K, N, D, R or I, preferably N;
X22 is Y, K, L, F or H, preferably K;
X23 is N, K, G or Q, preferably K; and X2~ is L or Y, preferably L.
Peptides exemplified herein are CMYGGVTEHEGN (SEQ
ID NO: 3), CMYGGVTEHEGNGC* (SEQ ID NO: 5), KKNVTVQELDYKIRKYLVDNKKLY (SEQ ID N0: 4), CGKKNVTVQELDYKIRKYLVDNKKLYGC* (SEQ ID NO: 6), CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLY (SEQ ID NO: 7) and CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC* (SEQ ID NO: 8), wherein an asterisk indicates that the peptide is a randomly cross-linked polymer. The exemplified polymer o peptides are at least 6,000 to 8,000 daltons. The average size of the exemplified polymer peptides is about 12,000 to 15,000 daltons. Small peptides and/or contaminants may be removed by dialysis or other methods available in the art. Similarly, larger aggregates may be removed usin g, e.g., a 0.25 micron filter, which can also be used to sterilize the peptides.
Note that the amino acids cysteine and methionine, "CM", are present at the amino terminus of the exemplified region 1 peptides since those amino acids are most often found in that position in nature. Note also that the amino acids cysteine and glycine, "CG" and "GC", are used at the amino and/or carboxy- termini of some of the exemplified region 2 peptides. The amino acid cysteine "C" is used to facilitate cross-linking through the formation of disulfide bonds. The amino acid glycine, "G", is used as a spacer residue.
The preferred peptides of the invention are those which exclude full length native toxin molecules. The preferred peptides of this invention are not toxic, but toxic peptides maybe useful in this invention, for example, in eliciting antibodies in a non-human system.
The most preferred peptides of the invention do not contain amino acid sequences in the sequence in which they are found in any particular native toxin molecule.
The present invention encompasses monomers of the peptides derived from either one or both of the two consensus regions described herein. These monomers may comprise one or more sequences derived from either region 1 or region 2 or both, such as consensus sequences #1 and #2, preferably consensus sequences #la and/or #2a, more preferably consensus sequences #lb o and/or #2b, most preferably one or more of the exemplified consensus sequence peptides. If the monomer contains more than one consensus sequence, these sequences may be immediately adjacent to each other or separated by a linker. In addition, different orientations of the peptides are within the scope of this invention. Furthermore, the order of the consensus peptides within the full peptide may be variable.
The present invention also encompasses homogeneous or heterogeneous polymers of the peptides disclosed herein (e. g., concatenated, cross-linked and/or fused identical peptide units or concatenated, cross-linked and/or fused diverse peptide units), and mixtures of the peptides, polymers, and/or conjugates thereof.
Linkers useful in the invention may, for example, be simply peptide bonds, or may comprise amino acids, including amino acids capable of forming disulfide bonds, but may also comprise other molecules such as, for example, polysaccharides or fragments thereof.
In the peptides exemplified herein, sequences derived from consensus region 1 and consensus region 2 may be immediately adjacent to each other, linked by peptide bonds, (see, era., SEQ ID N0:7) and/or connected via amino acid linkers capable of forming di-sulfide bonds via cysteine residues (see, e-a., SEQ ID N0: 8).
In the native toxin molecules, the sequences of region 1 and region 2 are separated by about 27 amino acids.
When the linkers are additional amino acids, they are most preferably 1 to 27 amino acids in length, although longer linkers may also be used in accordance with this invention.
The linkers for use with this invention may be o chosen so as to contribute their own immunogenic effect which may be either the same, or different, than that elicited by the consensus sequences of the invention.
For example, such linkers may be bacterial antigens which also elicit the production of antibodies to infectious bacteria. In such instances, for example, the linker may be a protein or protein fragment of an infectious bacteria, or a bacterial polysaccharide or polysaccharide fragment.
A peptide of the invention includes any substituted analog or chemical derivative of a peptide derived from one or both of the two consensus regions described herein, most preferably of the exemplified peptides described herein, so long as the peptide is capable of inhibiting binding of staphylococcal and streptococcal pyrogenic exotoxins to the MHC complex; inhibiting blastogenesis of human mononuclear cells in the presence of any one of the toxins; eliciting the production of antibodies capable of binding to most of the staphylococcal and streptococcal pyrogenic exotoxins; or reacting with (i.e., specifically binding to) antibodies that react with most of the staphylococcal and streptococcal pyrogenic exotoxins. Therefore, a peptide can be subject to various changes that provide for certain advantages in its use. For example, D amino acids can be substituted for L amino acids to increase in vivo stability of the peptides, while still retaining biological activity. See, e~a., Senderoff et al. (1998) (95). Likewise, retro-inverso peptides, which contain NH-CO bonds instead of CO-NH peptide bonds, are much more resistant to proteolysis than L-peptides.
Moreover, they have been shown to mimic natural L-o peptides with respect to poly- and monoclonal antibodies (48). Therefore, peptides having at least one D amino acid on the amino terminal and/or carboxy terminal end of the molecule and which retain biological activity are considered part of the invention. In addition, retro-inverso peptides which contain one or more of the amino acid sequences of the invention and which retain biological activity are also considered part of the invention.
The peptides of the invention are useful for providing active immunization for the prevention of disease related to the deleterious effects of staphylococcal and streptococcal pyrogenic exotoxins and for preparation of antibodies as a passive immunization therapy. When used to prepare antibodies, the peptides are designed to induce antibodies which react with a variety of staphylococcal and streptococcal pyrogenic exotoxins (preferably with at least two, more preferably with at least four, and most preferably with at least seven of the pyrogenic exotoxins) for use in therapy to increase resistance to, prevent and/or treat toxic shock syndrome and septic shock.
The peptides may also be useful to protect against, or ameliorate the effects of, autoimmune diseases which are associated with, or are the result of, the presence of staphylococcal or streptococcal exotoxins.
The peptides of the invention will also be useful in diagnostic tests for detecting antibodies to staphylococcal and streptococcal pyrogenic exotoxins.
The peptide may be mixed with an adjuvant. The peptide also may be bound to a non-toxic non-host Protein carrier to form a conjugate or it may be bound o to a saccharide carrier and/or a non-toxic non-host protein carrier to form a conjugate.
The molecular weight of the peptide monomers having one consensus sequence of the invention range from about 1000 to 5000 daltons. Such lower molecular weight species of the invention are useful themselves to inhibit superantigen induced T cell proliferation and/or reduce, inhibit or eliminate the deleterious effects of bacterial exotoxins in vivo, either when used alone or in combination with another form of therapy, e.g., anticytokine antibodies.
Such lower molecular weight species of the invention may also be useful as immunogens themselves or, more preferably, may be used as haptens conjugated to a larger carrier molecule, such as, for example, a protein. As with other peptides, the molecular weight of the peptide alone, or when conjugated to a carrier, or in the presence of an adjuvant, is related to its immunogenicity. Thus, the peptide may vary in molecular weight in order to enhance its antigenicity or immunogenicity. In an exemplified embodiment, the molecular weight of the peptide, in polymeric form, is greater than about 6000 to 8000 daltons, with an average weight of 12,000 to 15,000 daltons. The total size of the peptide is only limited to its ability to be physiologically tolerated.
The invention also relates to isolated and purified nucleic acid molecules which code for the peptides of the invention to produce the encoded peptides. The encoded peptides may be monomers, polymers or linked to other peptide sequences (e. g., they may be fusion proteins). Other features of the invention include WO 00/20598 PC'T/US99/22180 o vectors which comprise the nucleic acid molecules of the invention operably linked to promoters, as well as cell lines, such as prokaryotic (e.g., E. coli) and eukaryotic (e. g., CHO and COS) cells transfected with the nucleic acid molecules of the invention. Vectors and compositions for enabling production of the peptides in vivo, i.e., in the individual to be treated or immunized, are also within the scope of this invention.
The nucleic acids encoding the peptides of the invention can be introduced into a vector such as a plasmid, cosmid, phage, virus or mini-chromosome and inserted into a host cell or organism by methods well known in the art. In general, the vectors containing these nucleic acids can be utilized in any cell, either eukaryotic or prokaryotic, including mammalian cells (e. g., human (e. g., HeLa), monkey (e. g., COS), rabbit (e.g., rabbit reticulocytes), rat, hamster (e.g., CHO
and baby hamster kidney cells) or mouse cells (e.g., L
cells), plant cells, yeast cells, insect cells or bacterial cells (e.g., E. coli). The vectors which can be utilized to clone and/or express these nucleic acids are the vectors which are capable of replicating and/or expressing the nucleic acids in the host cell in which the nucleic acids are desired to be replicated and/or expressed. See, e.g., F. Ausubel et al., Current Protocols in Molecular Biolocrv, Greene Publishing Associates and Wiley-Interscience (1992) and Sambrook et al. (1989) for examples of appropriate vectors for various types of host cells. Strong promoters compatible with the host into which the gene is inserted may be used. These promoters may be inducible. The host cells containing these nucleic acids can be used to o express large amounts of the protein useful in pharmaceuticals, diagnostic reagents, vaccines and therapeutics.
The nucleic acids could be used, for example, in the production of peptides for diagnostic reagents, vaccines and thera ies for p pyrogenic exotoxin related diseases. For example, vectors expressing high levels of peptide can be used in immunotherapy and immunoprophylaxis, after expression in humans. Such vectors include retroviral vectors and also include direct injection of DNA into muscle cells or other receptive cells, resulting in the efficient expression of the peptide, using the technology described, for example, in Wolff et al., Science 247:1465-1468 (1990), ~"~olff et al. , Human Molecular Genetics 1 (6) :363-369 (1992) and Ulmer et al., Science 259:1745-1749 (1993).
See also, for example, WO 96/36366 and WO 98/34640.
In another embodiment of this invention antibodies are provided which react with peptides of the invention, as well as a variety of staphylococcal and streptococcal pyrogenic exotoxins (preferably with at least two, more preferably with at least four, and most preferably with at least seven of the pyrogenic exotoxins). These antibodies will be useful for passive immunization therapy to increase resistance to or prevent toxic shock syndrome or septic shock or other diseases related to the presence of bacterial pyrogenic exotoxin. The antibodies may also be useful to protect against, or ameliorate the effects of, autoimmune diseases which are associated with, or are the result of, the presence of staphylococcal or streptococcal pyrogenic exotoxins.
The antibodies of the invention will also be useful in o diagnostic tests and kits for detecting the presence of staphylococcal and streptococcal pyrogenic exotoxins.
These uses are discussed in more detail below.
Methods for t~reparincr peptides of the invention The peptides of the invention may be prepared by synthetic methods or by recombinant DNA methods, as known in the art and as described herein.
Pharmaceutical Compositions The pharmaceutical compositions of this invention contain a pharmaceutically and/or therapeutically effective amount of at least one peptide and/or carrier thereof, antibody, or nucleic acid encoding a peptide of this invention. In one embodiment of the invention, the effective amount or peptide per unit dose is an amount Sufficient to inhibit T-cell proliferation by staphylococcal and/or streptococcal pyrogenic exotoxins.
In another embodiment of the invention, the effective amount of peptide per unit dose is an amount sufficient to prevent, treat or protect against the toxic effects of bacterial toxins, including diarrhea and/or cardiopulmonary depression or lethal shock. The effective amount of peptide per unit dose depends, among other things, on the species of mammal inoculated, the body weight of the mammal and the chosen inoculation regimen, as is well known in the art.
In such circumstances, inocula for a human or similarly sized mammal typically contain peptide concentrations of 100 to 500 mgs/kg, body weight of the mammal per inoculation dose.
Preferably, the route of inoculation of the peptide will be subcutaneous or intravenous. The dose is administered at least once.
o When the peptide of the invention is used as immunogen, the pharmaceutical composition contains an effective, immunogenic, amount of peptide of the invention. The effective amount of peptide per unit dose sufficient to induce an immune response depends, among other things, on the species of mammal inoculated, the body weight of the mammal and the chosen inoculation regimen, as well as the presence or absence of an adjuvant, as is well known in the art. Inocula typically contain peptide concentrations of about 1 microgram to about 1000 micrograms per inoculation (dose), preferably about 3 micrograms to about 100 micrograms per dose, most preferably about 5 micrograms to 50 micrograms. The use of higher amounts is envisaged. In Example 1, rabbits were injected twice with 500 micrograms of polymeric peptide in the presence of adjuvant. In Example 5,an example in which the peptide is administered directly to prevent toxic or septic shock, which may not be dependent on the production of antibodies, mice were injected twice with 1.5 mg of monomer peptide for a total of 3 mgs.
Standard procedures to determine dose response relationships known to those skilled in the art may be used to determine optimum doses of peptide to be used either to prevent or treat toxic or septic shock, or to raise antibodies for its prevention or treatment.
The term "unit dose" as it pertains to the inocula refers to physically discrete units suitable as unitary dosages for mammals, each unit containing a predetermined quantity of active material (e. g., peptide, antibody or nucleic acid) calculated to produce the desired immunogenic effect in association with the o required diluent.
Inocula are typically prepared as a solution in a physiologically acceptable carrier such as saline, phosphate-buffered saline and the like to form an aqueous pharmaceutical composition.
The peptides of the invention are generally administered with a physiologically acceptable carrier or vehicle therefor. A physiologically acceptable carrier is one that does not cause an adverse physical reaction upon administration and one in which the antibodies are sufficiently soluble and retain their activity to deliver a therapeutically effective amount of the compound. The therapeutically effective amount and method of administration of a peptide of the invention may vary based on the individual patient, the indication being treated and other criteria evident to one of ordinary skill in the art. A therapeutically effective amount of a peptide of the invention is one sufficient to attenuate the dysfunction without causing significant side effects such as non-specific T cell lysis or organ damage. The routes) of administration useful in a particular application are apparent to one or ordinary skill in the art.
Routes of administration of the peptides include, but are not limited to, parenteral, and direct injection into an affected site. Parenteral routes of administration include but are not limited to intravenous, intramuscular, intraperitoneal and subcutaneous. The route of inoculation of the peptides of the invention is typically parenteral and is preferably intramuscular, sub-cutaneous and the like.
The present invention includes compositions of the o peptides described above, suitable for parenteral administration including, but not limited to, pharmaceutically acceptable sterile isotonic solutions.
Such solutions include, but are not limited to, saline and phosphate buffered saline for nasal, intravenous, intramuscular, intraperitoneal, subcutaneous or direct injection into a joint or other area.
A system for sustained delivery of the peptides of the invention may also be used. For example, a delivery system based on containing a peptide in a polymer matrix of biodegradable microspheres may be used (57). One such polymer matrix includes the polymer poly(lactide-co-glycolide) (PLG). PLG is biocompatible and can be given intravenously or orally. Following injection of the microspheres into the body, the encapsulated protein is released by a complex process involving hydration of the particles and drug dissolution. The duration of the release is mainly governed by the type of PLG polymer used and the release of modifying excipients (4~).
The dose is administered at least once. When a composition of the invention is used to induce antibodies, at least one booster dose may be administered after the initial injection, preferably at about 4 to 6 weeks after the first dose, in order to increase the antibody level. Subsequent doses may be administered as indicated.
To monitor the antibody response of individuals administered the compositions of the invention, antibody titers may be determined. In most instances it will be sufficient to assess the antibody titer in serum or plasma obtained from such an individual. Decisions as .to whether to administer booster inoculations or to o change the amount of the composition administered to the individual may be at least partially based on the titer.
The titer may be based on either an immunobinding assay which measures the concentration of antibodies in the serum which bind to a specific antigen, i.e. peptide or toxin; or bactericidal assays which measure the ability of the antibodies to participate with complement in killing bacteria. The ability to neutralize in vitro and in vivo biological effects of the pyrogenic exotoxins may also be assessed to determine the effectiveness of the treatment. See, e.g., the examples herein.
Antibodies The term "antibodies" is used herein to refer to immunoglobulin molecules and immunologically active portions of immunoglobulin molecules. Exemplary antibody molecules are intact immunoglobulin molecules, substantially intact immunoglobulin molecules and portions of an immunoglobulin molecule, including those portions known in the art as Fab, Fab', F(ab')z and F(v) as well as chimeric antibody molecules.
An antibody of the present invention is typically produced by immunizing a mammal with an immunogen or vaccine containing one or more peptides of the invention, or a structurally and/or antigenically related molecule, to induce, in the mammal, antibody molecules having immunospecificity for the immunizing peptide or peptides. The peptides) or related molecules) may be monomeric, polymeric, conjugated to a carrier, and/or administered in the presence of an adjuvant. The antibody molecules may then be collected from the mammal if they are to be used in immunoassays 0 or for providing passive immunity.
The antibody molecules of the present invention may be polyclonal or monoclonal. Monoclonal antibodies may be produced by methods known in the art. Portions of immunoglobulin molecules may also be produced by methods known in the art.
The antibody of the present invention may be contained in various carriers or media, including blood, plasma, serum (e. g., fractionated or unfractionated serum), hybridoma supernatants and the like.
Alternatively, the antibody of the present invention is isolated to the extent desired by well known techniques such as, for example, by using DEAE Sephadex, or affinity chromatography. The antibodies may be purified so as to obtain specific classes or subclasses of antibody such as IgM, IgG, IgA, IgGl, IgG2, IgG3, IgG4 and the like. Antibody of the IgG class are preferred for purposes of passive protection.
The presence of the antibodies of the present invention, either polyclonal or monoclonal, can be determined by various assays. Assay techniques include, but are not limited to, immunobinding, immunofluorescence (IF), indirect immunofluorescence, immunoprecipitation, ELISA, agglutination and Western blot techniques.
The antibodies of the present invention have a number of diagnostic and therapeutic uses. The antibodies can be used as an in vitro diagnostic agent to test for the presence of various staphylococcal and streptococcal pyrogenic exotoxins in biological samples in standard immunoassay protocols and to aid in the diagnosis of various diseases related to the presence of o bacterial pyrogenic exotoxins. Preferably, the assays which use the antibodies to detect the presence of bacterial pyrogenic exotoxins in a sample involve contacting the sample with at least one of the antibodies under conditions which will allow the formation of an immunological complex between the antibody and the toxin that may be present in the sample. The formation of an immunological complex if any, indicating the presence of the toxin in the sample, is then detected and measured by suitable means. Such assays include, but are not limited to, radioimmunoassays, (RIA), ELISA, indirect immunofluorescence assay, Western blot and the like.
The antibodies may be labeled or unlabeled depending on the type of assay used. Labels which may be coupled to the antibodies include those known in the art and include, but are not limited to, enzymes, radionucleotides, fluorogenic and chromogenic substrates, cofactors, biotin/avidin, colloidal gold and magnetic particles. Modification of the antibodies allows for coupling by any known means to carrier proteins or peptides or to known supports, for example, polystyrene or polyvinyl microliter plates, glass tubes or glass beads and chromatographic supports, such as paper, cellulose and cellulose derivatives, and silica.
Such assays may be, for example, of direct format (where the labelled first antibody reacts with the antigen), an indirect format (where a labelled second antibody reacts with the first antibody), a competitive format (such as the addition of a labelled antigen), or a sandwich format (where both labelled and unlabelled antibody are utilized), as well as other formats WO 00/20598 PC'T/US99/22180 o described in the art. In one such assay, the biological sample is contacted to antibodies of the present invention and a labelled second antibody is used to detect the presence of staphylococcal and streptococcal pyrogenic exotoxins, to which the antibodies are bound.
The antibodies of the present invention are also useful as therapeutic agents in the prevention and treatment of diseases caused by the deleterious effects of staphylococcal and streptococcal pyrogenic exotoxins.
The antibodies are generally administered with a physiologically acceptable carrier or vehicle therefor.
A physiologically acceptable carrier is one that does not cause an adverse physical reaction upon administration and one in which the antibodies are sufficiently soluble and retain their activity to deliver a therapeutically effective amount of the compound. The therapeutically effective amount and method of administration of the antibodies may vary based on the individual patient, the indication being treated and other criteria evident to one of ordinary skill in the art. A therapeutically effective amount of the antibodies is one sufficient to inhibit superantigen stimulation of T-cells and/or attenuate the dysfunction caused by the presence of bacterial toxins without causing significant side effects such as non-specific T
cell lysis or organ damage. The routes) of administration useful in a particular application are apparent to one or ordinary skill in the art.
Routes of administration of the antibodies include, but are not limited to, parenteral, and direct injection into an affected site. Parenteral routes of administration include but are not limited to o intravenous, intramuscular, intraperitoneal and subcutaneous.
The present invention includes compositions of the antibodies described above, suitable for parenteral administration including, but not limited to, pharmaceutically acceptable sterile isotonic solutions.
Such solutions include, but are not limited to, saline and phosphate buffered saline for nasal, intravenous, intramuscular, intraperitoneal, subcutaneous or direct injection into a joint or other area.
Antibodies for use to elicit passive immunity in humans are preferably obtained from other humans previously inoculated with compositions comprising one or more of the consensus amino acid sequences of the invention. Alternativel y, antibodies derived from other species may also be used. Such antibodies used in therapeutics suffer from several drawbacks such as a limited half-life and propensity to elicit an immune response. Several methods have been proposed to overcome these drawbacks. Antibodies made by these methods are encompassed by the present invention and are included herein. One such method is the "humanizing" of non-human antibodies by cloning the gene segment encoding the antigen binding region of the antibody to the human gene segments encoding the remainder of the antibody. Only the binding region of the antibody is thus recognized as foreign and is much less likely to cause an immune response. An article describing such antibodies is Reichmann et al., "Reshaping Human Antibodies for Therapy", Nature 332:323-327 (1988), which is incorporated herein by reference. See also, Queen et al., US Patent 5,585,089, which is incorporated o herein by reference.
In providing the antibodies of the present invention to a recipient mammal, preferably a human, the dosage of administered antibodies will vary depending upon such factors as the mammal's age, weight, height, sex, general medical condition, previous medical history and the like.
In general, it is desirable to provide the recipient with a dosage of antibodies which is in the range of from about 5 mg/kg to about 20 mg/kg body weight of the mammal, although a lower or higher dose may be administered. In general, the antibodies will be administered intravenously (IV) or intramuscularly (IM).
Intravenous immunoglobulin (IVIG) can generally be given with a loadin dose of 200 m k g g/ g, with monthly injections of 100 mg/kg. High-dose IVIG may be given at 400-800 mg/kg, for antibody-deficient patients. See, e.g., The Merck Manual of Diagnosis and Therapy, 16th Edition, (Berkow R and Fletcher AJ, Eds.), Merck Research Laboratories, Rahway, NJ (1992).
The peptides and/or antibodies of the present invention are intended to be provided to the recipient subject in an amount sufficient to prevent, or attenuate the severity, extent or duration of the deleterious effects of staphylococcal and streptococcal pyrogenic exotoxins.
The administration of the agents including peptide and antibody compositions of the invention may be for either "prophylactic" or "therapeutic" purpose. When provided prophylactically, the agents are provided in advance of any symptom. The prophylactic administration of the agent serves to prevent or ameliorate any o subsequent deleterious effects of staphylococcal and streptococcal pyrogenic exotoxins. When provided therapeutically, the agent is provided at (or shortly after) the onset of a symptom of infection with bacteria expressing staphylococcal or streptococcal pyrogenic exotoxins. The a ent of the g present invention may, thus, be provided either prior to the anticipated exposure to bacteria expressing staphylococcal or streptococcal pyrogenic exotoxin (so as to attenuate the anticipated severity, duration or extent of disease symptoms) or after the initiation of the infection. The agent may also be provided to individuals at high risk for getting an infection with bacteria expressing staphylococcal or streptococcal pyrogenic exotoxins.
Also envisioned are therapies based upon vectors, such as viral vectors containing nucleic acid sequences coding for the peptides described herein. These molecules, developed so that they do not provoke a pathological effect, will stimulate the immune system to respond to the peptides.
For all therapeutic, prophylactic and diagnostic uses, the peptide of the invention, alone or linked to a carrier, as well as antibodies and other necessary reagents and appropriate devices and accessories may be provided in kit form so as to be readily available and easily used.
Where immunoassays are involved, such kits may contain a solid support, such as a membrane (e. g., nitrocellulose), a bead, sphere, test tube, rod, and so forth, to which a receptor such as an antibody specific for the target molecule will bind. Such kits can also include a second receptor, such as a labelled antibody.
o Such kits can be used for sandwich assays to detect toxins. Kits for competitive assays are also envisioned.
The following examples illustrate certain embodiments of the present invention, but should not be construed as limiting its scope in any way. Certain modifications and variations will be apparent to those skilled in the art from the teachings of the foregoing disclosure and the following examples, and these are intended to be encompassed by the spirit and scope of the invention.
Peptides whose sequences are based on the two highly conserved regions of the staphylococcal and streptococcal pyrogenic exotoxins described herein were constructed. The sequences were based on alignments of the streptococcal pyrogenic exotoxins with the staphylococcal enterotoxins, and the amino acids used in positions with possible degeneracy were the amino acids most frequently found in these positions. Three of the peptides were then catenated and polymerized to produce peptides of greater than 8000 daltons (i.e., peptides 6343, 6345 and 6348, described below). As described further below, peptide 6348 was used to immunize rabbits, which produced high titer antibodies to this peptide. These antibodies were tested for the ability to recognize the streptococcal and staphylococcal pyrogenic exotoxins. Immunological assays (immunoblots) revealed that these antibodies recognized regions common to all the pyrogenic exotoxins. These antibodies were also tested for the ability to neutralize in vitro and o in vivo biological activity of the pyrogenic exotoxins.
These antibodies protected against the biological T-cell proliferation of these toxins in an in vitro blastogenesis assay using human mononuclear cell populations. The lethal effects of staphylococcal toxin SEB and streptococcal pyrogenic toxin SPEA in vivo were also completely blocked by mixing the antibodies with the toxin prior to injection.
Materials and Methods Construction of Synthetic Peptides:
Peptides were constructed by solid phase synthesis (20) using the modifications described by Houghton (10).
1 . GCG Consensus #1 YGGXITXzX3X4XSN ( SEQ ID NO : 1 ) Peptide #1 CMYGGVTEHEGN (SEQ ID N0:3) 2. GCG Consensus #2 KXsX~XeX9XlaX11X1zX13DX14X1sX1sRXmXls LXlgX2pX21X22X23X24Y ( SEQ ID NO : 2 ) peptide #2 KKNVTVQELDYKIRKYLVDNKKLY (SEQ
ID N0:4) As is evident above, synthetic peptides #1 and #2 are not native peptides, i.e., their sequences differ from those found in native toxins. Variations of these peptides have also been constructed in order to generate concatenated polymers of the peptides. These polymers were constructed by the addition of glycine and of additional cysteine residues to the amino- and/or carboxyl- termini of the initial 2 peptides, thus facilitating concatenation via disulfide bond formation (37, 38, 39) . The polvmerizec~ mn1 Pr."1 A~ ,.,o,.,o +-~,o., dialyzed to remove molecules with molecular weights less than 6000-8000 daltons. One polymeric construct is o composed of the monomer: CMYGGVTEHEGNGC (SEQ ID N0:5).
An additional polymer is composed of the peptide:
CGKKNVTVQELDYKIRKYLVDNKKLYGC (SEQ ID N0:6).
In the native toxin molecules, consensus region #1 precedes consensus region #2 by 27 amino acid residues (e.g. [consensus region 1] x27 [consensus region 2]).
We have constructed the peptide:
CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLY (SEQ ID N0:7).
Like the native toxin molecule, this peptide is representative of the two consensus regions joined together in the proper order (region 1 in the N terminal half, and region 2 in the C-terminal half of the molecule), however they are not separated by an additional 27 residues as they are in the native toxins.
We have also constructed concatenated polymers based on the monomer: CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC (SEQ
ID N0:8) .
ID Peptide 6343 CMYGGVTEHEGN (SEQ ID N0:3) 6344 CMYGGVTEHEGNGC* (SEQ ID N0:5) 6345 KKNVTVQELDYKIRKYLVDNKKLY (SEQ ID N0:4) 6346 CGKKNVTVQELDYKIRKYLVDNKKLYGC* (SEQ ID N0:6) 6347 CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLY (SEQ ID
N0:7) 6348 CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC* (SEQ ID
N0:8) Peptides with an (*) are cross-linked polymers composed of the described sequence. It is expected that monomers of these peptides will also be useful in the present invention.
o Generation of anti-peptide sera.
New Zealand White rabbits were immunized by subcutaneous injection with 500 ~.g of peptide in complete Freund's adjuvant. Additional booster injections of 500 ~,g in incomplete adjuvant was administered 4 weeks after the primary injections. Ten days after booster injections, the rabbits were bled, and the anti-peptide titers were determined by ELISA.
Staphylococcal enterotoxins, TSST-1, and j0 streptococcal pyrogenic exotoxins were purchased from Toxin Technology Inc. (Sarasota, FL).
Immunoblots Each of the staphylococcal and streptococcal pyrogenic exotoxins were electrophoresed through 10°s SDS
15 pAGE gels (16) and transferred to nitrocellulose for western blots (33). The western blots were developed using the rabbit anti-peptide 6348 serum (anti-pep 6348 or AP6348) diluted 1:5000, followed by goat anti-rabbit 20 (IgG) alkaline phosphate conjugate (Sigma).
Inhibition of blastoaenesis Human peripheral blood mononuclear cell (PBMC) preparations were stimulated by each of the staphylococcal enterotoxins and streptococcal pyrogenic exotoxins. 100 ng of toxin was used to stimulate PBMC
preparations at cell concentrations of 105 cells per well in 96 well microliter plates. Phytohemagglutinin (PHA) was used in place of the toxins as a positive mitogenic control. Cell culture medium was supplemented with either 10% normal rabbit serum (NRS) or AP6348 serum. Blastogenesis was assayed by incorporation of tritiated thymidine after 5 days of culture (22). All experiments were performed in triplicate.
o Passive protection of rabbits Female New Zealand White rabbits >lyr old were obtained from Hazelton Dutchland Labs, Inc. (Denver, PA). Rabbits were challenged with staphylococcal or streptococcal toxins at doses ranging from 50 to 100 ~Cg/kg, as previously described (24). Briefly, pyrogenic toxins were incubated with either 200 ul of normal rabbit serum or 200 ul of anti-pep #6348 serum for one hour prior to challenge. Toxin-serum mixtures were administered intravenously through the marginal ear veins. Normal control rabbits were treated in an identical manner, with isotonic saline substituted for the pyrogenic toxin. Four hours later, rabbits were given a sub-lethal dose (5 ~g/kg) of endotoxin (E. coli LPS, List Biological Laboratories, Inc., Campbell, CA).
Rabbits were monitored 72 h for clinical signs of toxic shock. These included elevated temperature, diarrhea, cardiopulmonary distress, and conjunctival injection.
Rabbits with severe toxic shock exhibiting cyanosis and temperatures less than 97°F were declared moribund.
Moribund rabbits were euthanized by administration of 5 ml pentobarbital sodium. All animal protocols were reviewed by the Laboratory Animal Research Center at the Rockefeller University.
Results ELISA assays As seen in Figure 4, rabbits raised significant antibody titers to peptide 6348. Similarly, rabbits receiving immunizations with peptides 6344 and 6346 also developed high titers.
wo oonos9s Pc r~s99n2~so -4$-o Recognition of staphylococcal and streptococcal toxins by anti-pep 6348 serum Western blots of the staphylococcal and streptococcal toxins were developed with anti-peptide 6348 serum followed by an anti-rabbit IgG alkaline phosphatase conjugate (Sigma). The results of the western blot shown in Figure 5 indicate the anti-peptide 6348 serum recognizes the conserved regions of the bacterial toxin molecules; SEA, SEB, SED, SEE, SPEA, and TSST-1. SEC did not show a significant reaction with anti-peptide 6348.
Blastogenesis inhibition The percentage of inhibition, of toxin mediated blastogenesis, by AP6348 was assayed. Tritiated thymidine incorporation by human PBMC stimulated with staphylococcal and streptococcal pyrogenic toxins was significantly inhibited by the addition of AP6348 compared to normal rabbit serum (NRS) (Figure 6). This suggests blastogenesis of PBMC in response to the toxins was inhibited by AP6348. The AP6348 serum did not affect the blastogenesis of human PBMC in response to PHA, suggesting a specific inhibition of toxin biologic activity.
In vivo protection of rabbits We tested the ability of AP6348 serum to prevent severe toxic shock in rabbits challenged with SEB and NRS. Rabbits challenged intravenously with a mixture of SEB and NRS developed symptoms of severe toxic shock (Table 2). One rabbit receiving 50 ~g/kg SEB with NRS, and two receiving 100 ~g/kg of SEB with NRS, developed severe toxic shock and were declared moribund within 30 o hrs. In contrast, two rabbits challenged with 50 ~g/kg and 100 ~g/kg SEB with AP6348 developed fever, but this returned to normal by 32 hours. No diarrhea or cardiopulmonary depression was observed. Rabbits were followed for a total of 5 days (data not shown) and appeared fully recovered.
Table 2 Passive Protection of Rabbits Challenged with SEB SPEA and LPS
Toxin LPS Diarrhea Temperature F
~.g/kg\Serum ~g/kg 0 hr 4 hr 24 hr 32 hr 48 hr SEB
nsf\NRS 5 - 100.4 102 101.4 101.2 NT
50\NRS 5 + 101 104.4 102.8 960 100\NRS S + 102 104.6 103 970 100\NRS 5 + 101 104.5 102.6 970 50\APS 5 - 101.4 103.8 103 102 101 100\APS 5 - 100.4 104.4 103 102 101 IS SPEA
50\NRS 5 + 101 104.2 NTO
100\NRS 5 + 102 104.8 NT0 50\APS 5 102 104 103 102 102 100\APS 5 + 101.6 104.4 104 100 970 ns =control rabbit isotonic saline in place of SEB or given SPEA
NRS=Normal rabbit serum APS=Anti-peptide 6348 serum 0=animals were declaredmoribund NT=not taken Discussion Our results demonstrate that antibodies rabbit antiserum generated to peptides representative of two regions with highly conserved amino acid sequences (AP6348) are capable of recognizing most of the staphylococcal enter otoxins and streptococcal pyrogenic exotoxins (e. g. SEA, SEB, SEC, SEE, SPEA, SPEC), as well as TSST-1, using Wes tern blots. We expect that other , o more sensitive assays, will result in the demonstration of binding of these antibodies to additional members, probably all members, of the staphylococcal and streptococcal pyrogenic toxin family.
Since recognition of the toxins by AP6348 was successful, we tested this serum for the ability to inhibit the biological effects of these pyrogenic toxins. AP6348 was capable of inhibiting in vitro blastogenesis of human PBMCs by many of the pyrogenic toxins (e. g., SEA, SEB, SEC, SEE, SPEA, and SPEC).
AP6348 was also able to provide passive in vivo protection of animals challenged with lethal doses of SEB and SPEA. These animals developed fever, however the fever returned to normal within 30 hours and remained normal. Rabbits appeared to be fully recovered within days of challenge.
In contrast, rabbits receiving similar doses of SEB
and SPEA pre-incubated with NRS developed severe toxic shock as evidenced by high fevers, diarrhea, and cardiopulmonary distress. The illness progressed and these animals were declared moribund.
The therapeutic and biological implications of these observations are as follows: (i) antibodies prepared against this peptide may be administered during the early stages of toxic shock or septic shock irrespective of the toxin causing the symptoms and (ii) the peptide may be used as an immunogen to block the toxic effects of this family of superantigens.
PBMCs were isolated via Ficoll-Hypaque Solution.
The appropriate concentration of nonpolymeric peptide o and 2x105 cells in 200~,L of RPMI solution was plated in each well. The cells were incubated for one hour at 37 degrees Centigrade, with mild agitation every 15 minutes. After one hour, 2~,g of SEB was added in each well. The PBMCs were incubated for 72 hours and the results were measured via tritiated thymidine incorporation. The cells were collected and read on a beta counter. The results are shown in Figure 7. Note the dose-response inhibition of blastogenesis demonstrated in Figure 7A. Peptide 6343 (i.e., CMYGGVTEGEGN, SEQ ID N0:3)(Figure 7A) showed more inhibitory activity of SEB than peptide 6346 (i.e., CGKKNVTVQELDYKIRKYLVDNKKLYGC, SEQ ID N0:6) (Figure 7B) or peptide 6348 (i.e., CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC, SEQ ID N0:8) (Figure 7C).
PBMCs were isolated via Ficoll-Hypaque Solution.
150~.g of nonpolymeric 6343 peptide (i.e., CMYGGVTEGEGN, SEQ ID N0:3) and 2x105 cells in 200~L of RPMI solution was plated in each well. The cells were incubated for one hour at 37 degrees Centigrade, with mild agitation zs every 15 minutes. After one hour, 2~.g of either SEB, SEC, SED, SPEC, SPEA, or TSST-1 was added to each well.
The PBMCs were incubated for 72 hours and the results were measured via tritiated thymidine incorporation.
The cells were collected and read on a beta counter.
All experiments were run in triplicate. The results are shown in Figure 8. Note that peptide 6343 inhibited blastogenesis of PBMCs by all of the superantigens tested.
Two-hit Septic Shock Model Based on the two hit septic shock hypothesis described in the background and Figure 2 we have created a model of septic shock. While the amounts of either SPEA or SEB superantigens used in the rabbit model were relatively high, the amounts used in the mouse model were much lower due to D-galactosamine priming, size of animals and synergy between the toxins and LPS. BALB/c mice challenged intra-peritoneally after priming with D-galactosamine (20 mg/mouse) concurrently with LPS
followed by SEB, showed that extremely small amounts of LPS and SEB were needed to effect lethality (46). The synergy between these two mediators of shock was extremely impressive and extended for at least an 18 hour period. We chose an 8 hour delay between the two toxins for our model. We established and optimized doses of toxin for SEA, SEB, SPEA, SPEC, and TSST-1 that would lead to 100% lethality. The doses of the various toxins are shown in Table 3.
Table 3: Doses of various toxins and LPS/ D-galactosamine used in the lethal two hit septic shock model Toxin (~.g) SEA SPEA SEB SPEC TSST-1 2.5 2.0 0.02 2.5 2.0 LPS (~.~g) 0.001 0.001 0.001 0.001 0.001 D-galactosamine 20 20 20 20 20 mg (mg) All mice were sensitized with 0,001 ~,g Lipopolysaccharide (LPS) and 20 mg of D-Galactosamine via intraperioneal injection. The results are shown in Table 4. After six hours, saline or 1.5 mg of the non of meric p y peptide 6343 was administered to the experimental mice by subcutaneous injection. One hour later, the mice were injected again with either saline or 1.5 mg peptide (3.0 mg total). One hour later, all mice were challenged with 0.02 ~.g SEB, SPEA or TSST-1 (via intraperitoneal injection) and the mice were observed overnight. In this model, it has been observed that peptide 6343, given one and two hours before administration of the toxic dose of the indicated toxin, protected 5 out of 6 mice exposed to toxin SEB; 2 out of 2 mice exposed to the toxin SPEA and 2 out of two mice exposed to the toxin TSST-1.
Table 4 The Use of Peptide 6343 to Block the Superantigen Induction of Septic Shock in a Mouse Model Mice Dose A me Tota A me Tota A me Tota and AdministrationSEB SPEA TSST-1 ControlSaltine 0 6 (0% 0 2 0%) 0 2 (0%) subcutaneous injection Peptide 5 6 83%) 2 2 (100% 2 2 (100%
3.0 mg subcutaneous injection Two streptococcal antigens SEG and SEH have recently been synthesized and three new streptococcal exotoxins, i.e., SPEG, SPEH and SPEZ, have recently been described by Dr. Fraser and colleagues (61).
In order to determine whether 6343 peptide is capable of inhibiting the toxic effects of the streptococcal exotoxins SPEG, SPEH and SPEZ, experiments similar to those described above were conducted.
In a similar manner as above, PBMCs were isolated via Ficoll-Hypaque Solution. Either O~g, 75~Cg, 100~g or 150~,g of nonpolymeric 6343 peptide (i.e., CMYGGVTEGEGN, SEQ ID N0:3) and 2x205 cells in 200~L of RPMI solution IS was plated in each well. The cells were incubated for one hour at 37 degrees Centigrade, with mild agitation every 15 minutes. After one hour, 2~.g of the recombinant forms of either SPEG, SPEH, SPEZ, which were provided by Dr. Fraser of the University of Auckland, New Zealand, was added to each well. The PBMCs were incubated for 72 hours and the results were measured via tritiated thymidine incorporation. The cells were collected and read on a beta counter. The results are shown in Figure 9. Note that peptide 6343 inhibited blastogenesis of PBMCs by the bacterial superantigens SPEG, SPEH and SPEZ.
To determine the nature of the inhibition of superantigen stimulation by the inhibitory peptides and antigens, purified MHC class II molecules (obtained from J. Strominger, Harvard University) were used in a competitive ELISA to determine binding to this molecule.
o The purified MHC were immobilized on a 95 well plate and 4~.g SEB was added. After appropriate washing a rabbit monoclonal anti-SEB antibody (Toxin Tech) was added to the ELISA followed by a colorimetric reagent. The plate was read on an ELISA plate reader. In similar experiments various concentrations of the peptide 6343 ( i . a . , O~g, 50~.g, 75~.g, 100~,g, or 150~Cg) were added to the immobilized MHC before the addition of SEB. Binding of the peptide to the MHC was demonstrated by the decreased amount of SEB bound to the MHC, as indicated by a decreased amount of antibody binding measured by a decreased color reaction. The results are shown in Figure 11. The results indicate that peptide 6343 binds very strongly to the MHC complex and is able to compete effectively for the site of SEB binding.
The binding of the peptide to the MHC complex of monocytes was supported by con-focal microscopy results of experiments using a method described in Ojcius et al.
(68) using fluorinated peptides constructed for us by NEN LifeSciences, antibodies directed against MHC class II proteins, and a phycoerythrin (PE) antibody directed to the anti-MHC antibody. Various doses of the peptide and various concentrations of the cells were titrated to achieve optimum binding of the peptide. Confocal microscope pictures (Figure 11) show similar patterns of binding for anti-MHC antibodies and for the flourinated peptides, supporting the determination that the peptide binds to the cells' MHC complex.
° DISCUSSION
In the experiments described herein, the dose of peptide 6343 used directly for the prevention of toxic and septic shock was 3 mgs per mouse. Thus, the direct use of peptides in septic or toxic shock would be expected to involve doses in the range of several grams for the acute treatment of shock in humans. Peptide 6343, possibly because of its small size, does not induce detectable antibodies in rabbits and mice, yet it still has all the therapeutic properties described for the anti-peptide antibodies. Therefore, if desired, it is expected that the peptide can be used repeatedly in the same individual without raising anti-peptide antibodies.
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86. Brocke S, Gaur A, Piercy C, et al:
Induction of relapsing paralysis in experimental autoimmune encephalomyelitis by bacterial superantigen.
Nature 365:642-644, 1993.
87. Kotzin BL, Leung DY, Kappler J, et al:
Superantigens and their potential role in human disease.
Adv Immunol 54:99-166, 1993.
88. Li S, Quayle AJ, Thoen JE, et al:
Superantigen-mediated proliferation and cytotoxicity of T cells isolated from the inflammatory tissues and peripheral blood of arthritis patients. Clin Immunol Immunopathol 79:278-287, 1996.
89. Schiffenbauer J, Johnson HM, Butfiloski EJ, et al: Staphylococcal enterotoxins can reactivate experimental allergic encephalomyelitis. Proc Natl Acad Sci USA 90:8543-8546, 1993.
90. Schleivert PM: Role of superantigens in human disease. J Infect Dis 167:997-1002, 1993.
91. Schwab J, Brown R, Anderle S, et al:
Superantigen can reactivate baacterial cell wall-induced arthritis. J Immunol 150:4151-4159, 1993.
92. Astiz M, Saha D, Lustbader D, et al:
Monocyte response to bacterial toxins, expression of cell surface receptors, and release of anti-inflammatory cytokines during sepsis. J Lab Clin Med 128:594-600, 1996 .
93. Schleivert PM, Bohach GA, Ohlendorf DH, et al: Molecular structure of staphylococcus and streptococcus superantigens. J Clin Immunol 15:4s-lOs, 1995.
0 94. Barman J, Visvanathan K, and Zabriskie JB. "Structure and function of streptococcal and staphylococcal superantigens in Septic Shock,"
Infectious Disease Clinics of North America 13(2):387-396, 1999.
95. Senderoff RI, Kontor KM, Kreilgaard L, Chang JJ, Patel S, Krakover J, Heffernan JK, Snell LB, Rosenberg GB. Consideration of conformational transitions and racemization during process development of recombinant glucagon-like peptide-1. Journal of Pharmaceutical Sciences. 87(2):183-9, 1998 96. Rangel-Frausto MS, Pittet D, Costigan M, Hwang T, Davis CS, Wenzel RP. The natural history of the systemic inflammatory response syndrome (SIRS). A
prospective study. JAMA 273(2):117-23, 1995 Every reference cited hereinbefore is hereby incorporated by reference in its entirety.
Modifications of the above described modes for carrying out the invention that are obvious to those of skill in the fields of immunology, protein chemistry, microbiology, medicine, and related fields are intended to be within the scope of the following claims.
The data suggest a theory for a possible mechanism of action which is discussed in the application.
However, the application describes how to make and use the invention. This description is not dependent upon theory and, accordingly, the claims are not bound by theory.
The following table shows the correspondence between peptides in Figure 3 and their sequence identification numbers:
Table 5 Correspondence between Sequence Identification Numbers and Peptides in Figure 3 Figure 3 Sequence ID
Nos.
Region PEP CMYGGVTEHEGN SEQ ID N0:3 SEA 130 CMYGGVTLHDNN 141 SEQ ID N0:9 SEB 140 CMYGGVTEHNGN 151 SEQ ID NO:10 SEC 137 CMYGGITKHEGN 148 SEQ ID NO:11 SED 131 CTYGGVTPHEGN 142 SEQ ID N0:12 SEE 130 CMYGGVTLHDNN 141 SEQ ID N0:13 SEH 116 CLYGGITL.NSE 126 SEQ ID N0:14 SPEA 128 CIYGGVTNHEGN 139 SEQ ID N0:15 SPEC 112 YIYGGIT'PAQNN 123 SEQ ID N0:16 SSA 134 CMYGGVTEHHRN 145 SEQ ID N0:17 Region PEP KKNVTVQELDYKIRKYLVDNKKLY SEQ ID N0:4 SEA 171 KKNVTVQELDLQARRYLQEKYNLY 194 SEQ ID N0:18 SEB 179 KKKVTAQELDYLTRHYLVKNKKLY 202 SEQ ID N0:19 SEC 178 KKSVTAQELDIKARNFLINKKNLY 201 SEQ ID N0:20 SED 172 KKNVTVQELDAQARRYLQKDLKLY 195 SEQ ID N0:21 SEE 171 KKEVTVQELDLQARHYLHGKFGLY 194 SEQ ID N0:22 SEH 151 KKNVTLQELDIKIRKILSDKYKIY 174 SEQ ID N0:23 SPEA 167 KKMVTAQELDYKVRKYLTDNKQLY 190 SEQ ID N0:24 SPEC 151 KDIVTFQEIDFKIRKLYMDNYKIY 174 SEQ ID N0:25 SSA 174 KKQVTVQELDCKTRKILVSRKNLY 197 SEQ ID N0:26 TSST1 161 KKQLAISTLDFEIRHQLTQIHGLY 184 SEQ ID N0:27 o SEQUENCE LISTING
(1) GENERAL INFORMATION:
(i) APPLICANT:
(A) NAME: The Rockefeller University (B) STREET: 1230 York Avenue (C) CITY: New York (D) STATE OR PROVINCE: New York (E) COUNTRY: UNITED STATES OF AMERICA
(F) POSTAL CODE: 10021-6399 (ii) TITLE OF INVENTION: PEPTIDES USEFUL FOR
REDUCING SYMPTOMS OF TOXIC SHOCK
SYNDROME AND SEPTIC SHOCK
(iii) NUMBER OF SEQUENCES: 31 (iv) CORRESPONDENCE ADDRESS:
(A) ADDRESSEE: MORGAN & FINNEGAN
(B) STREET: 345 PARK AVENUE
(C) CITY: NEW YORK
(D) STATE: NEW YORK
(E) COUNTRY: USA
(F) ZIP: 10154 (v) COMPUTER READABLE FORM:
(A) MEDIUM TYPE: FLOPPY DISK
(B) COMPUTER: IBM PC COMPATIBLE
(C) OPERATING SYSTEM: MS-WINDOWS
(D) SOFTWARE: MS WORD 95 (vi) CURRENT APPLICATION DATA:
(A) APPLICATION NUMBER: TO BE ASSIGNED
(B) FILING DATE: 24 SEPTEMBER 1999 (C) CLASSIFICATION:
(vii) PRIOR APPLICATION DATA:
(A) APPLICATION NUMBER: 09/335,581 (B) FILING DATE: 18 JUNE 1999 (C) CLASSIFICATION:
(vii) PRIOR APPLICATION DATA:
(A) APPLICATION NUMBER: 09/168,303 (8) FILING DATE: 07 OCTOBER 1998 (C) CLASSIFICATION:
(vii) PRIOR APPLICATION DATA:
WO 00!20598 PCT/US99/22180 o (A) APPLICATION NUMBER: 08/838,413 (B) FILING DATE: 07 APRIL 1997 (C) CLASSIFICATION:
(viii) ATTORNEY/AGENT INFORMATION:
(A) NAME: MORRY, MARY J.
(B) REGISTRATION NUMBER: 34,398 S (C) REFERENCE/DOCKET NUMBER: 2016-4010PC2 (ix) TELECOMMUNICATION INFORMATION:
(A) TELEPHONE: (212)758-4800 (B) TELEFAX: (212)751-6849 (2) INFORMATION FOR SEQ ID NO: 1:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 10 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTIONS:SEQ ID NO: 1:
Tyr Gly Gly Xaa Thr Xaa Xaa Xaa Xaa Asn (3) INFORMATION FOR SEQ ID NO: 2:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTIONS:SEQ ID NO: 2:
Lys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Asp Xaa Xaa Xaa Arg Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Tyr (4) INFORMATION FOR SEQ ID NO: 3:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 (B) TYPE: AMINO ACID
{C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTIONS:SEQ ID NO: 3:
Cys Met Tyr Gly Gly Val Thr Glu His Glu Gly Asn {5) INFORMATION FOR SEQ ID NO: 4:
(i) SEQUENCE CHARACTERISTICS:
{A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
{D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTIONS:SEQ ID NO: 4:
Lys Lys Asn Val Thr Val G:ln Glu Leu Asp Tyr Lys Ile Arg Lys Tyr Leu Val Asp Asn Lys Lys Leu Tyr (6) INFORMATION FOR SEQ ID NO: 5:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 14 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTIONS:SEQ ID NO: 5:
o Cys Met Tyr Gly Gly Val Thr Glu His Glu Gly Asn Gly Cys (7) INFORMATION FOR SEQ ID NO: 6:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 28 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6:
Cys Gly Lys Lys Asn Val Thr Val Gln Glu Leu Asp Tyr Lys Ile Arg Lys Tyr Leu Val Asp Asn Lys Lys Leu Tyr Gly Cys (8) INFORMATION FOR SEQ ID NO: 7:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 36 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
25 (D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 7:
CYs Met Tyr Gly Gly Val Thr Glu His Glu Gly Asn Lys Lys Asn Val Thr Val Gln Glu Leu Asp Tyr Lys Ile Arg Lys Tyr Leu Val Asp Asn Lys Lys Leu Tyr _77_ (9) INFORMATION FOR SEQ ID NO: 8:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 38 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8:
Cys Met Tyr Gly Gly Val Thr Glu His Glu Gly Asn Lys Lys Asn Val Thr Val Gln Glu Leu Asp Tyr Lys IS Ile Arg Lys Tyr Leu Val Asp Asn Lys Lys Leu Tyr Gly Cys (10) INFORMATION FOR SEQ ID NO: 9:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE 'DESCRIPTION: SEQ ID NO: 9:
Cys Met Tyr Gly Gly Val Thr Leu His Asp Asn Asn (11) INFORMATION FOR SEQ ID NO: 10:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
_78_ o (D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10:
Cys Met Tyr Gly Gly Val Thr Glu His Asn Gly Asn (12) INFORMATION FOR SEQ ID NO: 11:
(i) SEQUENCE CHARACTERISTICS:
10 (A) LENGTH: 12 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11:
Cys Met Tyr Gly Gly Ile Thr Lys His Glu Gly Asn (13) INFORMATION FOR SEQ ID NO: 12:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 12:
Cys Thr Tyr Gly Gly Val Thr Pro His Glu Gly Asn (14) INFORMATION FOR SEQ ID NO: 13:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 (B) TYPE: AMINO ACID
o (C) STRANDEDNESS: UNKNOWN
(D} TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13:
S Cys Met Tyr Gly Gly Val Thr Leu His Asp Asn Asn (15) INFORMATION FOR SEQ ID NO: 14:
(i) SEQUENCE CHARACTERISTICS:
10 (A) LENGTH: 11 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14:
Cys Leu Tyr Gly Gly Ile Thr Leu Asn Ser Glu (16) INFORMATION FOR SEQ ID NO: 15:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 15:
CYs Ile Tyr Gly Gly Val Thr Asn His Glu Gly Asn (17) INFORMATION FOR SEQ ID NO: 16:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 o (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 16:
Tyr Ile Tyr Gly Gly Ile Thr Pro Ala Gln Asn Asn (18) INFORMATION FOR SEQ ID NO: 17:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 17:
Cys Met Tyr Gly Gly Val Thr Glu His His Arg Asn 5 1. 0 (19) INFORMATION FOR SEQ ID NO: 18:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID N0: 18:
LYs Lys Asn Val Thr Val Gln Glu Leu Asp Leu Gln Ala Arg Arg Tyr Leu Gln Glu Lys Tyr Asn Leu Tyr 35 (20) INFORMATION FOR SEQ ID NO: 19:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
{xi) SEQUENCE DESCRIPTION: SEQ ID NO: 19:
Lys Lys Lys Val Thr Ala Gln Glu Leu Asp Tyr Leu Thr Arg His Tyr Leu Val Lys Asn Lys Lys Leu Tyr (21) INFORMATION FOR SEQ ID NO: 20:
is (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID N0: 20:
Lys Lys Ser Val Thr Ala Gln Glu Leu Asp Ile Lys Ala Arg Asn Phe Leu Ile Asn Lys Lys Asn Leu Tyr (22) INFORMATION FOR SEQ ID NO: 21:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 21:
Lys Lys Asn Val Thr Val Gln Glu Leu Asp Ala Gln Ala Arg Arg Tyr Leu Gln Lys Asp Leu Lys Leu Tyr (23) INFORMATION FOR SEQ ID NO: 22:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH:
(B) TYPE: AMINO ACID
10 (C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22:
15 LYs Lys Glu Val Thr Val Gln Glu Leu Asp Leu Gln Ala Arg His Tyr Leu His Gly Lys Phe Gly Leu Tyr (24) INFORMATION FOR SEQ ID NO: 23:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID N0: 23:
Lys Lys Asn Val Thr Leu Gln Glu Leu Asp Ile Lys Ile Arg Lys Ile Leu Ser Asp Lys Tyr Lys Ile Tyr (25) INFORMATION FOR SEQ ID NO: 24:
o (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 24:
Lys Lys Met Val Thr Ala Gln Glu Leu Asp Tyr Lys Val Arg Lys Tyr Leu Thr Asp Asn Lys Gln Leu Tyr (26) INFORMATION FOR SEQ ID NO: 25:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 25:
Lys Asp Ile Val Thr Phe Gln Glu Ile Asp Phe Lys Ile Arg Lys Leu Tyr Met Asp Asn Tyr Lys Ile Tyr (27) INFORMATION FOR SEQ ID NO: 26:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 3O (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 26:
WO 00/20598 PCf/US99/22180 -$4-o Lys Lys Gln Val Thr Val Gln Glu Leu Asp Cys Lys Thr Arg Lys Ile Leu Val Ser Arg Lys Asn Leu Tyr 5 (28) INFORMATION FOR SEQ ID NO: 27:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
10 (D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 27:
Lys Lys Gln Leu Ala Ile Ser Thr Leu Asp Phe Glu Ile Arg His Gln Leu Thr Gln Ile His Gly Leu Tyr (29) INFORMATION FOR SEQ ID NO: 28:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 28:
Xaa Xaa Tyr Gly Gly Xaa Thr Xaa Xaa Xaa Xaa Asn (30) INFORMATION FOR SEQ ID NO: 29:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
-$5-o (C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 29:
Lys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Asp Xaa Xaa Xaa Arg Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Tyr (31) INFORMATION FOR SEQ ID NO: 30:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH; 12 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 30:
Cys Met Tyr Gly Gly Xaa Thr Xaa His Xaa Gly Asn ZO
(32) INFORMATION FOR SEQ ID NO: 31:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 31:
Lys Lys Xaa Val Thr Xaa Gln Glu Leu Asp Xaa Xaa Xaa Arg Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Leu Tyr
Radermacher (1998). "Outcome of patients with sepsis and septic shock after ICU treatment." Langenbecks Arch Surg 383 (1) : 44-8.
0 64. Wang, M.H., H.D. Flad, W. Feist, J.
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66. Weiss, K.A. and M. Laverdiere (1997).
"Group A Streptococcus invasive infections: a review."
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67. Woodley, J.F. (1994). "Enzymatic barriers for GI peptide and protein delivery." Crit Rev Ther Drug Carrier Syst 11(2-3): 61-95.
68. Ojcius, D.M., F. Niedergang, A. Subtil, R. Hellio, and A. Dautry-Varsat (1996). "Immunology and the confocal microscope." Research in Immunology 147(3):175-188.
69. Soos, JM, Johnson HM: Multiple binding sites on the superantigen, staphylococcal enterotoxin B, imparts versatility in binding to MHC class II
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70. Yagi J, Rath S, Janeway CA, Jr: Control of T cell responses to staphylococcal enterotoxins by stimulator cell MHC class II polymorphism. J Immunol 147:13998-11405,1991.
71. Muller-Alouf H, Alouf JE, Gerlach D, et al: Human pro- and anti-inflammaotry cytokine patterns o induced by Streptococcus pyogenes erythrogenic (pyrogenic) exotoxin A and C superantigens. Infect Immun 64:1450-1453, 1996.
72. Leung DY, Travers JB, Giorno R, et al:
Evidence for a streptococcal superantigen-driven process in acute guttate psoriasis. J Clin Invest 96:2106-2112, 1995.
73. Muller-Alouf H, Alouf JE, Gerlach D, et al: Comparative study of cytokine release by human peripheral blood mononuclear cells stimulated with Streptococcus pyogenes superantigenic erythrogenic toxins, heat killed streptococci, and lipopolysaccharide. Infect Immun 62:4915-4921, 1994.
74. Blankson JN, Morse SS: The CD28/B7 pathway cosimulates the response of primary murine T
cells to superantigens as well as to conventional antigens. Cell Immunol 157:306-312, 1994.
75. Fleischer B, Schrezenmeier H: T cell stimulation by staphylococcal enterotoxins. Clonally variable response and requirement for major histocompatibility complex class II molecules on accessory or target cells. J Exp Med 167:1697, 1988.
76. Krakauer T: Cell adhesion molecules are co-receptors for staphylococcal enterotoxin B-induced T-cell activation and cytokine production. Immunol Lett 39:121-125, 1994.
77. van Seventer GA, Newman W, Shimizu Y, et al: Analysis of T cell stimulation by superantigen plus major histocompatibility complex class II molecules or by CD3 monoclonal antibody: Costimulation by purified adhesion ligands VCAM-1 ICAM-1 but not ELAM-1. J Exp Med 174:901-913, 1991.
WO 00/20598 PCf/US99/22180 c 78. Chapes SK, Beharka AA, Hart ME, et al:
Differential RNA regulation by staphylococcal enterotoxins A and B in murine macrophages. J Leukoc Biol 55:523-529, 1994.
79. Hackett SP, Stevens DL: Superantigens associated with staphylococcal and streptococcal toxic shock syndrome are potent inducers of tumor necrosis factor beta synthesis. J Infect Dis 1.68:232-235, 1993.
80. Imanishi K, Akatsuka H, Inada K, et al:
IFN-gamma-stimulated human vascular endothelial cells function as accessory cells for superantigen-induced TNF
production in human T-cells. Int Arch Allergy Immunol 106:163-165, 1995.
81. Blank C, Luz A, Bendigs S, et al:
superantigens and endotoxin synergize in the induction of lethal shock. Eur J Immunol 27:825-833, 1997 82. Leonard BA, Schlievert PM: Immune cell lethality induced by streptococcal pyrogenic exotoxin A
and endotoxin. Infect Immun 60:3747-3755, 1992.
83. Sugiyama H, McKissic EMJ, Bergdoll MS, et al: Enhancement of bacterial endotoxin lethality by staphylococcal enterotoxin. J Infect Dis 114:111-118, 1964.
84. Hensler T, Koller M, Geoffroy C, et al:
Staphylococcus aureus toxic shock syndrome toxin 1 and Streptococcus pyogenes erythrogenic toxin A modulate inflammatory mediator release from human neutrophils.
Infect Immun 61:1055-1061, 1993.
85. Smith RJ, Schlievert PM, Himelright IM, et al: Dual infections with Staphylococcus aureus and Streptococcus pyogenes causing toxic shock syndrome.
Possible synergistic effects of toxic shock syndrome o toxin 1 and streptococcal pyrogenic exotoxin C. Diagn Microbiol Infect Dis 19:245-247, 1994.
86. Brocke S, Gaur A, Piercy C, et al:
Induction of relapsing paralysis in experimental autoimmune encephalomyelitis by bacterial superantigen.
Nature 365:642-644, 1993.
87. Kotzin BL, Leung DY, Kappler J, et al:
Superantigens and their potential role in human disease.
Adv Immunol 54:99-166, 1993.
88. Li S, Quayle AJ, Thoen JE, et al:
Superantigen-mediated proliferation and cytotoxicity of T cells isolated from the inflammatory tissues and peripheral blood of arthritis patients. Clin Immunol Immunopathol 79:278-287, 1996.
89. Schiffenbauer J, Johnson HM, Butfiloski EJ, et al: Staphylococcal enterotoxins can reactivate experimental allergic encephalomyelitis. Proc Natl Acad Sci USA 90:8543-8546, 1993.
90. Schleivert PM: Role of superantigens in human disease. J Infect Dis 167:997-1002, 1993.
91. Schwab J, Brown R, Anderle S, et al:
Superantigen can reactivate baacterial cell wall-induced arthritis. J Immunol 150:4151-4159, 1993.
92. Astiz M, Saha D, Lustbader D, et al:
Monocyte response to bacterial toxins, expression of cell surface receptors, and release of anti-inflammatory cytokines during sepsis. J Lab Clin Med 128:594-600, 1996 .
93. Schleivert PM, Bohach GA, Ohlendorf DH, et al: Molecular structure of staphylococcus and streptococcus superantigens. J Clin Immunol 15:4s-lOs, 1995.
0 94. Barman J, Visvanathan K, and Zabriskie JB. "Structure and function of streptococcal and staphylococcal superantigens in Septic Shock,"
Infectious Disease Clinics of North America 13(2):387-396, 1999.
95. Senderoff RI, Kontor KM, Kreilgaard L, Chang JJ, Patel S, Krakover J, Heffernan JK, Snell LB, Rosenberg GB. Consideration of conformational transitions and racemization during process development of recombinant glucagon-like peptide-1. Journal of Pharmaceutical Sciences. 87(2):183-9, 1998 96. Rangel-Frausto MS, Pittet D, Costigan M, Hwang T, Davis CS, Wenzel RP. The natural history of the systemic inflammatory response syndrome (SIRS). A
prospective study. JAMA 273(2):117-23, 1995 Every reference cited hereinbefore is hereby incorporated by reference in its entirety.
Modifications of the above described modes for carrying out the invention that are obvious to those of skill in the fields of immunology, protein chemistry, microbiology, medicine, and related fields are intended to be within the scope of the following claims.
The data suggest a theory for a possible mechanism of action which is discussed in the application.
However, the application describes how to make and use the invention. This description is not dependent upon theory and, accordingly, the claims are not bound by theory.
The following table shows the correspondence between peptides in Figure 3 and their sequence identification numbers:
Table 5 Correspondence between Sequence Identification Numbers and Peptides in Figure 3 Figure 3 Sequence ID
Nos.
Region PEP CMYGGVTEHEGN SEQ ID N0:3 SEA 130 CMYGGVTLHDNN 141 SEQ ID N0:9 SEB 140 CMYGGVTEHNGN 151 SEQ ID NO:10 SEC 137 CMYGGITKHEGN 148 SEQ ID NO:11 SED 131 CTYGGVTPHEGN 142 SEQ ID N0:12 SEE 130 CMYGGVTLHDNN 141 SEQ ID N0:13 SEH 116 CLYGGITL.NSE 126 SEQ ID N0:14 SPEA 128 CIYGGVTNHEGN 139 SEQ ID N0:15 SPEC 112 YIYGGIT'PAQNN 123 SEQ ID N0:16 SSA 134 CMYGGVTEHHRN 145 SEQ ID N0:17 Region PEP KKNVTVQELDYKIRKYLVDNKKLY SEQ ID N0:4 SEA 171 KKNVTVQELDLQARRYLQEKYNLY 194 SEQ ID N0:18 SEB 179 KKKVTAQELDYLTRHYLVKNKKLY 202 SEQ ID N0:19 SEC 178 KKSVTAQELDIKARNFLINKKNLY 201 SEQ ID N0:20 SED 172 KKNVTVQELDAQARRYLQKDLKLY 195 SEQ ID N0:21 SEE 171 KKEVTVQELDLQARHYLHGKFGLY 194 SEQ ID N0:22 SEH 151 KKNVTLQELDIKIRKILSDKYKIY 174 SEQ ID N0:23 SPEA 167 KKMVTAQELDYKVRKYLTDNKQLY 190 SEQ ID N0:24 SPEC 151 KDIVTFQEIDFKIRKLYMDNYKIY 174 SEQ ID N0:25 SSA 174 KKQVTVQELDCKTRKILVSRKNLY 197 SEQ ID N0:26 TSST1 161 KKQLAISTLDFEIRHQLTQIHGLY 184 SEQ ID N0:27 o SEQUENCE LISTING
(1) GENERAL INFORMATION:
(i) APPLICANT:
(A) NAME: The Rockefeller University (B) STREET: 1230 York Avenue (C) CITY: New York (D) STATE OR PROVINCE: New York (E) COUNTRY: UNITED STATES OF AMERICA
(F) POSTAL CODE: 10021-6399 (ii) TITLE OF INVENTION: PEPTIDES USEFUL FOR
REDUCING SYMPTOMS OF TOXIC SHOCK
SYNDROME AND SEPTIC SHOCK
(iii) NUMBER OF SEQUENCES: 31 (iv) CORRESPONDENCE ADDRESS:
(A) ADDRESSEE: MORGAN & FINNEGAN
(B) STREET: 345 PARK AVENUE
(C) CITY: NEW YORK
(D) STATE: NEW YORK
(E) COUNTRY: USA
(F) ZIP: 10154 (v) COMPUTER READABLE FORM:
(A) MEDIUM TYPE: FLOPPY DISK
(B) COMPUTER: IBM PC COMPATIBLE
(C) OPERATING SYSTEM: MS-WINDOWS
(D) SOFTWARE: MS WORD 95 (vi) CURRENT APPLICATION DATA:
(A) APPLICATION NUMBER: TO BE ASSIGNED
(B) FILING DATE: 24 SEPTEMBER 1999 (C) CLASSIFICATION:
(vii) PRIOR APPLICATION DATA:
(A) APPLICATION NUMBER: 09/335,581 (B) FILING DATE: 18 JUNE 1999 (C) CLASSIFICATION:
(vii) PRIOR APPLICATION DATA:
(A) APPLICATION NUMBER: 09/168,303 (8) FILING DATE: 07 OCTOBER 1998 (C) CLASSIFICATION:
(vii) PRIOR APPLICATION DATA:
WO 00!20598 PCT/US99/22180 o (A) APPLICATION NUMBER: 08/838,413 (B) FILING DATE: 07 APRIL 1997 (C) CLASSIFICATION:
(viii) ATTORNEY/AGENT INFORMATION:
(A) NAME: MORRY, MARY J.
(B) REGISTRATION NUMBER: 34,398 S (C) REFERENCE/DOCKET NUMBER: 2016-4010PC2 (ix) TELECOMMUNICATION INFORMATION:
(A) TELEPHONE: (212)758-4800 (B) TELEFAX: (212)751-6849 (2) INFORMATION FOR SEQ ID NO: 1:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 10 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTIONS:SEQ ID NO: 1:
Tyr Gly Gly Xaa Thr Xaa Xaa Xaa Xaa Asn (3) INFORMATION FOR SEQ ID NO: 2:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTIONS:SEQ ID NO: 2:
Lys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Asp Xaa Xaa Xaa Arg Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Tyr (4) INFORMATION FOR SEQ ID NO: 3:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 (B) TYPE: AMINO ACID
{C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTIONS:SEQ ID NO: 3:
Cys Met Tyr Gly Gly Val Thr Glu His Glu Gly Asn {5) INFORMATION FOR SEQ ID NO: 4:
(i) SEQUENCE CHARACTERISTICS:
{A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
{D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTIONS:SEQ ID NO: 4:
Lys Lys Asn Val Thr Val G:ln Glu Leu Asp Tyr Lys Ile Arg Lys Tyr Leu Val Asp Asn Lys Lys Leu Tyr (6) INFORMATION FOR SEQ ID NO: 5:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 14 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTIONS:SEQ ID NO: 5:
o Cys Met Tyr Gly Gly Val Thr Glu His Glu Gly Asn Gly Cys (7) INFORMATION FOR SEQ ID NO: 6:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 28 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6:
Cys Gly Lys Lys Asn Val Thr Val Gln Glu Leu Asp Tyr Lys Ile Arg Lys Tyr Leu Val Asp Asn Lys Lys Leu Tyr Gly Cys (8) INFORMATION FOR SEQ ID NO: 7:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 36 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
25 (D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 7:
CYs Met Tyr Gly Gly Val Thr Glu His Glu Gly Asn Lys Lys Asn Val Thr Val Gln Glu Leu Asp Tyr Lys Ile Arg Lys Tyr Leu Val Asp Asn Lys Lys Leu Tyr _77_ (9) INFORMATION FOR SEQ ID NO: 8:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 38 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8:
Cys Met Tyr Gly Gly Val Thr Glu His Glu Gly Asn Lys Lys Asn Val Thr Val Gln Glu Leu Asp Tyr Lys IS Ile Arg Lys Tyr Leu Val Asp Asn Lys Lys Leu Tyr Gly Cys (10) INFORMATION FOR SEQ ID NO: 9:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE 'DESCRIPTION: SEQ ID NO: 9:
Cys Met Tyr Gly Gly Val Thr Leu His Asp Asn Asn (11) INFORMATION FOR SEQ ID NO: 10:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
_78_ o (D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10:
Cys Met Tyr Gly Gly Val Thr Glu His Asn Gly Asn (12) INFORMATION FOR SEQ ID NO: 11:
(i) SEQUENCE CHARACTERISTICS:
10 (A) LENGTH: 12 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11:
Cys Met Tyr Gly Gly Ile Thr Lys His Glu Gly Asn (13) INFORMATION FOR SEQ ID NO: 12:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 12:
Cys Thr Tyr Gly Gly Val Thr Pro His Glu Gly Asn (14) INFORMATION FOR SEQ ID NO: 13:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 (B) TYPE: AMINO ACID
o (C) STRANDEDNESS: UNKNOWN
(D} TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13:
S Cys Met Tyr Gly Gly Val Thr Leu His Asp Asn Asn (15) INFORMATION FOR SEQ ID NO: 14:
(i) SEQUENCE CHARACTERISTICS:
10 (A) LENGTH: 11 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14:
Cys Leu Tyr Gly Gly Ile Thr Leu Asn Ser Glu (16) INFORMATION FOR SEQ ID NO: 15:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 15:
CYs Ile Tyr Gly Gly Val Thr Asn His Glu Gly Asn (17) INFORMATION FOR SEQ ID NO: 16:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 o (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 16:
Tyr Ile Tyr Gly Gly Ile Thr Pro Ala Gln Asn Asn (18) INFORMATION FOR SEQ ID NO: 17:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 17:
Cys Met Tyr Gly Gly Val Thr Glu His His Arg Asn 5 1. 0 (19) INFORMATION FOR SEQ ID NO: 18:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID N0: 18:
LYs Lys Asn Val Thr Val Gln Glu Leu Asp Leu Gln Ala Arg Arg Tyr Leu Gln Glu Lys Tyr Asn Leu Tyr 35 (20) INFORMATION FOR SEQ ID NO: 19:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
{xi) SEQUENCE DESCRIPTION: SEQ ID NO: 19:
Lys Lys Lys Val Thr Ala Gln Glu Leu Asp Tyr Leu Thr Arg His Tyr Leu Val Lys Asn Lys Lys Leu Tyr (21) INFORMATION FOR SEQ ID NO: 20:
is (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID N0: 20:
Lys Lys Ser Val Thr Ala Gln Glu Leu Asp Ile Lys Ala Arg Asn Phe Leu Ile Asn Lys Lys Asn Leu Tyr (22) INFORMATION FOR SEQ ID NO: 21:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 21:
Lys Lys Asn Val Thr Val Gln Glu Leu Asp Ala Gln Ala Arg Arg Tyr Leu Gln Lys Asp Leu Lys Leu Tyr (23) INFORMATION FOR SEQ ID NO: 22:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH:
(B) TYPE: AMINO ACID
10 (C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22:
15 LYs Lys Glu Val Thr Val Gln Glu Leu Asp Leu Gln Ala Arg His Tyr Leu His Gly Lys Phe Gly Leu Tyr (24) INFORMATION FOR SEQ ID NO: 23:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID N0: 23:
Lys Lys Asn Val Thr Leu Gln Glu Leu Asp Ile Lys Ile Arg Lys Ile Leu Ser Asp Lys Tyr Lys Ile Tyr (25) INFORMATION FOR SEQ ID NO: 24:
o (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 24:
Lys Lys Met Val Thr Ala Gln Glu Leu Asp Tyr Lys Val Arg Lys Tyr Leu Thr Asp Asn Lys Gln Leu Tyr (26) INFORMATION FOR SEQ ID NO: 25:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 25:
Lys Asp Ile Val Thr Phe Gln Glu Ile Asp Phe Lys Ile Arg Lys Leu Tyr Met Asp Asn Tyr Lys Ile Tyr (27) INFORMATION FOR SEQ ID NO: 26:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 3O (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 26:
WO 00/20598 PCf/US99/22180 -$4-o Lys Lys Gln Val Thr Val Gln Glu Leu Asp Cys Lys Thr Arg Lys Ile Leu Val Ser Arg Lys Asn Leu Tyr 5 (28) INFORMATION FOR SEQ ID NO: 27:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
10 (D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 27:
Lys Lys Gln Leu Ala Ile Ser Thr Leu Asp Phe Glu Ile Arg His Gln Leu Thr Gln Ile His Gly Leu Tyr (29) INFORMATION FOR SEQ ID NO: 28:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 28:
Xaa Xaa Tyr Gly Gly Xaa Thr Xaa Xaa Xaa Xaa Asn (30) INFORMATION FOR SEQ ID NO: 29:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
-$5-o (C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 29:
Lys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Asp Xaa Xaa Xaa Arg Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Tyr (31) INFORMATION FOR SEQ ID NO: 30:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH; 12 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 30:
Cys Met Tyr Gly Gly Xaa Thr Xaa His Xaa Gly Asn ZO
(32) INFORMATION FOR SEQ ID NO: 31:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 (B) TYPE: AMINO ACID
(C) STRANDEDNESS: UNKNOWN
(D) TOPOLOGY: UNKNOWN
(ii) MOLECULE TYPE: PEPTIDE
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 31:
Lys Lys Xaa Val Thr Xaa Gln Glu Leu Asp Xaa Xaa Xaa Arg Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Leu Tyr
Claims (49)
1. A peptide comprising a consensus amino acid sequence selected from the group consisting of X25X26YGGX1TX2X3X4X5N (SEQ ID NO: 28) and KX6X7X8X9X10X11X12X13DX14X15X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO: 29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18 X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y.
NO: 29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18 X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y.
2. A peptide of claim 1 wherein X2 is selected from the group consisting of L, E, K, P and N;
X3 is selected from the group consisting of H
and A or no amino acid;
X4 is selected from the group consisting of D, N, E, Q, and H;
X5 is selected from the group consisting of N, G, S, and R;
X6 is selected from the group consisting of K
and D;
X7, is selected from the group consisting of N, K, S, E, M, I and Q;
X9 is selected from the group consisting of T
and A;
X10 is selected from the group consisting of V, A, L, F and I;
X11 is selected from the group consisting of Q
and S;
X12 is selected from the group consisting of E
and T;
X14 is selected from the group consisting of L, Y, I, A, F and C;
X15 is selected from the group consisting of Q, L, K and E;
X16 is selected from the group consisting of A, T, I and V;
X17 is selected from the group consisting of R, H, N and K;
X18 is selected from the group consisting of Y, F, T, L and Q;
X19 is selected from the group consisting of Q, V, I, H, S, T and M;
X20 is selected from the group consisting of E, K, N, G, D, S and Q;
X21 is selected from the group consisting of K, N, D, R and I;
X22 is selected from the group consisting of Y, K, L, F and H;
X23 is selected from the group consisting of N, K, G and Q;
X25 is selected from the group consisting of C, Y and no amino acid; and X26 is selected from the group consisting of M, T, L, I and no amino acid.
X3 is selected from the group consisting of H
and A or no amino acid;
X4 is selected from the group consisting of D, N, E, Q, and H;
X5 is selected from the group consisting of N, G, S, and R;
X6 is selected from the group consisting of K
and D;
X7, is selected from the group consisting of N, K, S, E, M, I and Q;
X9 is selected from the group consisting of T
and A;
X10 is selected from the group consisting of V, A, L, F and I;
X11 is selected from the group consisting of Q
and S;
X12 is selected from the group consisting of E
and T;
X14 is selected from the group consisting of L, Y, I, A, F and C;
X15 is selected from the group consisting of Q, L, K and E;
X16 is selected from the group consisting of A, T, I and V;
X17 is selected from the group consisting of R, H, N and K;
X18 is selected from the group consisting of Y, F, T, L and Q;
X19 is selected from the group consisting of Q, V, I, H, S, T and M;
X20 is selected from the group consisting of E, K, N, G, D, S and Q;
X21 is selected from the group consisting of K, N, D, R and I;
X22 is selected from the group consisting of Y, K, L, F and H;
X23 is selected from the group consisting of N, K, G and Q;
X25 is selected from the group consisting of C, Y and no amino acid; and X26 is selected from the group consisting of M, T, L, I and no amino acid.
3. A peptide of claim 1 wherein X1 is valine; X2 is leucine; X3 is histidine; X4 is glutamic acid; X5 is glycine; X6 is lysine; X7 is asparagine; X8 is valine; X9 is threonine; X10 is valine; X11 is glutamine; X12 is glutamic acid; X13 is leucine; X14 is leucine, tyrosine, isoleucine or phenylalanine; X15 is lysine; X16 is alanine or isoleucine; X17 is lysine; X18 is tyrosine; X19 is glutamine, valine or threonine; X20 is aspartic acid; X21 is lysine; X22 is lysine; X23 is lysine; X24 is leucine; X25 is cysteine; X26 is methionine and X27 is leucine.
4. A peptide comprising at least one amino acid sequence selected from the group consisting of CMYGGVTEHEGN (SEQ ID NO: 3), CMYGGVTEHEGNGC (SEQ ID NO:
5), KKNVTVQELDYKIRKYLVDNKKLY (SEQ ID NO: 4), CGKKNVTVQELDYKIRKYLVDNKKLYGC (SEQ ID NO: 6), CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLY (SEQ ID NO: 7), and CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC (SEQ ID NO: 8).
5), KKNVTVQELDYKIRKYLVDNKKLY (SEQ ID NO: 4), CGKKNVTVQELDYKIRKYLVDNKKLYGC (SEQ ID NO: 6), CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLY (SEQ ID NO: 7), and CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC (SEQ ID NO: 8).
5. A peptide of claim 4 wherein the peptide comprises the amino acid sequence CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC (SEQ ID NO: 8).
6. A peptide of claim 4 wherein the amino acid sequence is a component of a larger molecule of at least 6,000 to 8,000 daltons.
7. A pharmaceutical composition comprising a peptide comprising a consensus amino acid sequence selected from the group consisting of X25X26YGGX1TX2X3X4X5N
(SEQ ID NO: 28) and KX6X7X8X9X10X11X12X13DX14X15X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO: 29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18 X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y;
in a physiologically acceptable carrier.
(SEQ ID NO: 28) and KX6X7X8X9X10X11X12X13DX14X15X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO: 29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18 X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y;
in a physiologically acceptable carrier.
8. A pharmaceutical composition comprising a peptide of claim 4 in a physiologically acceptable carrier.
9. A method of inducing serum antibodies that bind at least one staphylococcal enterotoxin or streptococcal exotoxin, said method comprising administering to a mammal, in a physiologically acceptable carrier, an amount of a peptide comprising a consensus amino acid sequence selected from the group consisting of X25X26YGGX1TX2X3X4X5N (SEQ ID NO: 28) and KX6X7X8X9X10X11X12X13DX14X15X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO: 29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18 X19, X20 X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y;
sufficient to elicit production of said antibodies.
NO: 29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18 X19, X20 X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y;
sufficient to elicit production of said antibodies.
10. The method of claim 9 wherein said peptide is administered at a dose of about 5 micrograms to about 100 micrograms.
11. A method of inducing serum antibodies that bind at least one staphylococcal enterotoxin or streptococcal exotoxin, said method comprising administering to a mammal, in a physiologically acceptable carrier, an amount of a peptide of claim 4 sufficient to elicit production of said antibodies.
12. The method of claim 11 wherein the peptide comprises the amino acid sequence CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC (SEQ ID NO: 8).
13. The method of claim 11 wherein said peptide is administered at a dose of about 5 micrograms to about 100 micrograms.
14. A method of inducing serum antibodies which detect the toxins SPEA, SEA, SEB, and SED, comprising administering to a mammal, in a physiologically acceptable carrier, an immunologically sufficient amount of a peptide comprising a consensus amino acid sequence selected from the group consisting of X25X26YGGX1TX2X3X4X5N (SEQ ID NO:28) and KX6X7X8X9X10X11X12X13DX14X15X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO:29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18, X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y.
NO:29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18, X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y.
15. A method of inducing serum antibodies which detect the toxins SPEA, SEA, SEB, and SED, comprising administering to a mammal, in a physiologically acceptable carrier, an immunologically sufficient amount of a peptide of claim 4.
16. The method of claim 15 wherein the peptide comprises the amino acid sequence CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC (SEQ ID NO: 8).
17. A method of inducing serum antibodies which inhibit blastogenesis of human mononuclear cells in the presence of any one of the toxins SEA, SEB, SEC, SEE, SPEA or SPEC comprising administering to a mammal, in a physiologically acceptable carrier, an immunologically sufficient amount of an antibody from a mammal immunized with a peptide comprising a consensus amino acid sequence selected from the group consisting of X25X26YGGX1TX2X3X4X5N (SEQ ID NO:28) and KX6X7X8X9X10X11X12X13DX14X15X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO:29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X8, X10, X11, X12, X14, X15, X16, X17, X18 X19, X20 X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X2 is selected from the group consisting of L and Y.
NO:29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X8, X10, X11, X12, X14, X15, X16, X17, X18 X19, X20 X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X2 is selected from the group consisting of L and Y.
18. A method of inducing serum antibodies which inhibit blastogenesis of human mononuclear cells in the presence of any one of the toxins SEA, SEB, SEC, SEE, SPEA or SPEC comprising administering to a mammal, in a physiologically acceptable carrier, an immunologically sufficient amount of an antibody from a mammal immunized with a peptide of claim 4.
19. The method of claim 18 wherein the peptide comprises the amino acid sequence CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC (SEQ ID NO: 8).
20. A method of passively immunizing a mammal against the toxic effects of staphylococcal and streptococcal toxins comprising: administering in vivo an immunologically sufficient amount of an antibody containing composition wherein said antibody is derived from the immunization of antibody producing cells with a peptide comprising a consensus amino acid sequence selected from the group consisting of X25X26YGGX1TX2X3X4XN
(SEQ ID NO:28) and KX6X7X8X9X10X11X12X13DX14X1X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO:29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18, X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y.
(SEQ ID NO:28) and KX6X7X8X9X10X11X12X13DX14X1X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO:29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18, X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y.
21. A method of passively immunizing a mammal against the toxic effects of staphylococcal and streptococcal toxins comprising administering in vivo an immunologically sufficient amount of an antibody containing composition wherein said antibody is derived from the immunization of antibody producing cells with a peptide of claim 4.
22. The method of claim 21 wherein the peptide comprises the amino acid sequence CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC (SEQ ID NO: 8).
23. The method of claim 20 or claim 21 wherein the antibody composition is administered at a dose in the range of from about 1 mg/kg to about 10 mg/kg body weight of the mammal.
24. The method of any one of claims 9, 11, 14, 15, 17, 18, 20 and 21 wherein the mammal is a human.
25. A nucleic acid encoding a peptide comprising a consensus amino acid sequence selected from the group consisting Of X25X26YGGX1TX2X3X4X5N (SEQ ID
NO:28) and KX6X7X8X9X10X11X12X13DX14X15X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO:29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18 X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y.
NO:28) and KX6X7X8X9X10X11X12X13DX14X15X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO:29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18 X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y.
26. A nucleic acid encoding at least one amino acid sequence of claim 4.
27. A nucleic acid of claim 26 wherein the amino acid sequence encoded by said nucleic acid comprises CMYGGVTEHEGNKKNVTVQELDYKIRKYLVDNKKLYGC (SEQ ID
NO: 8).
NO: 8).
28. A host cell containing the nucleic acid of claim 25.
29. A host cell containing the nucleic acid of claim 26.
30. A method of inducing serum antibodies that bind staphylococcal enterotoxin and streptococcal exotoxin comprising administering to a mammal, in a physiologically acceptable carrier, a nucleic acid of claim 25 which produces an immunologically sufficient amount of the encoded peptide to elicit said antibodies.
31. A method of inducing serum antibodies that bind staphylococcal enterotoxin and streptococcal exotoxin comprising administering to a mammal, in a physiologically acceptable carrier, a nucleic acid of claim 26 which produces an immunologically sufficient amount of the encoded peptide to elicit said antibodies.
32. An antibody made by the method of any one of claims 9, 11, 14, 15, 17, and 19.
33. A method for detecting the presence of staphylococcal or streptococcal toxin in a sample comprising contacting said sample with an antibody of claim 32 and detecting the antibody bound to said toxin.
34. A method for detecting the presence of antibodies to staphylococcal or streptococcal toxins in a sample comprising contacting said sample with a peptide comprising a consensus amino acid sequence selected from the group consisting of X25X26YGGX1TX2X3X4X5N
(SEQ ID NO:28) and KX6X7X8X9X10X11X12X13DX14X15X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO:29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18, X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y;
and detecting the peptide bound to said antibodies.
(SEQ ID NO:28) and KX6X7X8X9X10X11X12X13DX14X15X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO:29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18, X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y;
and detecting the peptide bound to said antibodies.
35. A method for detecting the presence of antibodies to staphylococcal or streptococcal toxins in a sample comprising contacting said sample with a peptide of claim 4 and detecting the peptide bound to said antibodies.
36. A kit for detecting the presence of staphylococcal or streptococcal toxins in a sample comprising an antibody of claim 32.
37. A kit for detecting the presence of antibodies to staphylococcal or streptococcal toxins in a sample comprising a peptide comprising a consensus amino acid sequence selected from the group consisting of X25X26YGGX1TX2X3X4X5N (SEQ ID NO:28) and KX6X7X8X9X10X11X12X13DX14X15X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO:29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18, X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y.
NO:29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18, X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y.
38. A kit for detecting the presence of antibodies to staphylococcal or streptococcal toxins in a sample comprising a peptide of claim 4.
39. A method of inhibiting blastogenesis of human mononuclear cells in the presence of any one of the toxins SEA, SEB, SEC, SEE, SPEA or SPEC comprising administering to a mammal, in a physiologically acceptable carrier, an effective amount a peptide comprising a consensus amino acid sequence selected from the group consisting of X25X26YGGX1TX2X3X4X5N (SEQ ID
NO:28) and KX6X7X8X9X10X11X12X13DX14X15X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO:29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18, X19, X20.
X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y.
NO:28) and KX6X7X8X9X10X11X12X13DX14X15X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO:29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18, X19, X20.
X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y.
40. A method of inhibiting blastogenesis of human mononuclear cells in the presence of any one of the toxins SEA, SEB, SEC, SEE, SPEA or SPEC comprising administering to a mammal, in a physiologically acceptable carrier, an effective amount of a peptide of claim 4.
41. The method of claim 40 wherein the peptide comprises the amino acid sequence CMYGGVTEHEGN
(SEQ ID NO: 3).
(SEQ ID NO: 3).
42. A method of inhibiting blastogenesis of human mononuclear cells in the presence of any one of the toxins SPEG, SPEH and SPEZ comprising administering to a mammal, in a physiologically acceptable carrier, an effective amount a peptide comprising a consensus amino acid sequence selected from the group consisting of X25X26YGGX1TX2X3X4X5N (SEQ ID NO:28) and KX6X7X8X9X10X11X12X13DX14X15X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO:29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18 X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y.
NO:29) wherein X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18 X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y.
43. A method of inhibiting blastogenesis of human mononuclear cells in the presence of any one of the toxins SPEC, SPEH and SPEZ comprising administering to a mammal, in a physiologically acceptable carrier, an effective amount of a peptide of claim 4.
44. The method of claim 40 wherein the peptide comprises the amino acid sequence CMYGGVTEHEGN
(SEQ ID NO: 3).
(SEQ ID NO: 3).
45. A method of protecting a mammal against the toxic effects of staphylococcal and streptococcal toxins comprising: administering in vivo a therapeutically sufficient amount of a peptide comprising a consensus amino acid sequence selected from the group consisting of X25X26YGGX1TX2X3X4X5N (SEQ ID
NO:28) and KX6X7X8X9X10X11X12X13DX14X15X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO:29) where in X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18 X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y.
NO:28) and KX6X7X8X9X10X11X12X13DX14X15X16RX17X18X27X19X20X21X22X23X24Y (SEQ ID
NO:29) where in X1, X8, X13 and X24 are each independently selected from the group consisting of L, I and V; X2, X4, X5, X6, X7, X9, X10, X11, X12, X14, X15, X16, X17, X18 X19, X20, X21, X22, and X23 are each independently selected from the group consisting of any amino acid; X3, X25 and X26 are each independently selected from the group consisting of any amino acid and of no amino acid; and X27 is selected from the group consisting of L and Y.
46. A method of protecting a mammal against the toxic effects of staphylococcal and streptococcal toxins comprising: administering in vivo a therapeutically sufficient amount of a peptide of claim 4.
47. The method of claim 46 wherein the peptide comprises the amino acid sequence CMYGGVTEHEGN
(SEQ ID NO: 3).
(SEQ ID NO: 3).
48. The method of claim 45 or claim 46 wherein the peptide composition is administered at a dose in the range of from about 100 mg/kg to 500 mg/kg body weight of the mammal.
49. The method of any one of claims 39-48 wherein the mammal is a human.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US16830398A | 1998-10-07 | 1998-10-07 | |
US09/168,303 | 1998-10-07 | ||
US09/335,581 US7115268B1 (en) | 1997-04-07 | 1999-06-18 | Peptides useful for reducing symptoms of toxic shock syndrome and septic shock |
US09/335,581 | 1999-06-18 | ||
PCT/US1999/022180 WO2000020598A1 (en) | 1998-10-07 | 1999-09-24 | Peptides useful for reducing symptoms of toxic shock syndrome and septic shock |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2345023A1 true CA2345023A1 (en) | 2000-04-13 |
Family
ID=26863977
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002345023A Abandoned CA2345023A1 (en) | 1998-10-07 | 1999-09-24 | Peptides useful for reducing symptoms of toxic shock syndrome and septic shock |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1127132A1 (en) |
JP (1) | JP2003517445A (en) |
AU (1) | AU6059799A (en) |
CA (1) | CA2345023A1 (en) |
WO (1) | WO2000020598A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
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US6550882B2 (en) | 2000-11-17 | 2003-04-22 | Canon Kabushiki Kaisha | Ink jet printing apparatus |
CA2924155C (en) | 2007-01-03 | 2020-02-11 | Philip M. Sass | High affinity antibodies that neutralize staphylococcus enterotoxin b |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
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ATE304371T1 (en) * | 1990-01-17 | 2005-09-15 | David S Terman | USE OF STAPHYLOCOCCUS ENTEROTOXIN HOMOLOGUE FOR CANCER THERAPY |
US5935568A (en) * | 1995-05-18 | 1999-08-10 | National Jewish Medical & Research Center | Gene therapy for effector cell regulation |
KR100456594B1 (en) * | 1995-06-07 | 2005-06-17 | 리전츠 오브 더 유니버스티 오브 미네소타 | Mutants of streptococcal toxin a and methods of use |
US6075119A (en) * | 1997-04-07 | 2000-06-13 | The Rockefeller University | Peptides useful for reducing symptoms of toxic shock syndrome |
-
1999
- 1999-09-24 EP EP99970123A patent/EP1127132A1/en not_active Withdrawn
- 1999-09-24 CA CA002345023A patent/CA2345023A1/en not_active Abandoned
- 1999-09-24 AU AU60597/99A patent/AU6059799A/en not_active Abandoned
- 1999-09-24 JP JP2000574693A patent/JP2003517445A/en active Pending
- 1999-09-24 WO PCT/US1999/022180 patent/WO2000020598A1/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
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JP2003517445A (en) | 2003-05-27 |
AU6059799A (en) | 2000-04-26 |
WO2000020598A1 (en) | 2000-04-13 |
WO2000020598A9 (en) | 2000-09-21 |
EP1127132A1 (en) | 2001-08-29 |
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