CA2344625A1 - Procedes de manipulation de populations d'acides nucleiques au moyen d'oligonucleotides a marquage peptidique - Google Patents

Procedes de manipulation de populations d'acides nucleiques au moyen d'oligonucleotides a marquage peptidique Download PDF

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Publication number
CA2344625A1
CA2344625A1 CA002344625A CA2344625A CA2344625A1 CA 2344625 A1 CA2344625 A1 CA 2344625A1 CA 002344625 A CA002344625 A CA 002344625A CA 2344625 A CA2344625 A CA 2344625A CA 2344625 A1 CA2344625 A1 CA 2344625A1
Authority
CA
Canada
Prior art keywords
label
cdna
library
dna
peptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002344625A
Other languages
English (en)
Inventor
Francois J.-M. Iris
Jean-Louis Pourny
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Valigen US Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2344625A1 publication Critical patent/CA2344625A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Abstract

L'invention concerne généralement des procédés de marquage, de tri et de criblage de populations d'acides nucléiques. Elle porte notamment sur un procédé de tri et de comparaison de populations complexes d'acides nucléiques, telle que des banques d'ADNc. Ces populations complexes peuvent être dérivées de cellules ou de types de tissus comportant des variations du phénotype présentant un éventuel intérêt clinique. Le procédé est généralement appelé VG-PLO?SM¿, procédé à Oligonucléotides à marquage peptidique, ou et implique l'utilisation d'étiquettes peptidiques identifiables, liées à des amorces olignonucléotidiques identiques.
CA002344625A 1998-10-16 1999-10-15 Procedes de manipulation de populations d'acides nucleiques au moyen d'oligonucleotides a marquage peptidique Abandoned CA2344625A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US17432898A 1998-10-16 1998-10-16
US09/174,328 1998-10-16
PCT/US1999/023906 WO2000023622A1 (fr) 1998-10-16 1999-10-15 Procedes de manipulation de populations d'acides nucleiques au moyen d'oligonucleotides a marquage peptidique

Publications (1)

Publication Number Publication Date
CA2344625A1 true CA2344625A1 (fr) 2000-04-27

Family

ID=22635773

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002344625A Abandoned CA2344625A1 (fr) 1998-10-16 1999-10-15 Procedes de manipulation de populations d'acides nucleiques au moyen d'oligonucleotides a marquage peptidique

Country Status (7)

Country Link
EP (1) EP1121470A1 (fr)
JP (1) JP2002527118A (fr)
KR (1) KR20010102909A (fr)
CN (1) CN1342208A (fr)
AU (1) AU1113000A (fr)
CA (1) CA2344625A1 (fr)
WO (1) WO2000023622A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002014867A2 (fr) * 2000-08-11 2002-02-21 Agilix Corporation Systemes de detection ultrasensibles
KR101110013B1 (ko) * 2007-10-05 2012-02-29 (주)바이오니아 서열 내에 어베이직 부분을 포함하는 ｐｃｒ 증폭용프라이머
EP2427210A4 (fr) * 2009-05-08 2012-10-24 Inst Medical W & E Hall Modulation de la liaison de l'oxyde nitrique synthase inductible (inos) à des peptides socs-box (ssb) contenant des domaines spry
AU2022354606A1 (en) * 2021-09-30 2024-04-11 Illumina, Inc. Blocker methods

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2318288A (en) * 1987-08-07 1989-03-09 Genelabs Incorporated Coincidence cloning method and library
US5804382A (en) * 1996-05-10 1998-09-08 Beth Israel Deaconess Medical Center, Inc. Methods for identifying differentially expressed genes and differences between genomic nucleic acid sequences
EP0839214A1 (fr) * 1996-05-14 1998-05-06 Roche Diagnostics GmbH Procede d'identification et/ou de quantification de l'expression de molecules d'acide nucleique dans un echantillon
US6221585B1 (en) * 1998-01-15 2001-04-24 Valigen, Inc. Method for identifying genes underlying defined phenotypes

Also Published As

Publication number Publication date
WO2000023622A1 (fr) 2000-04-27
JP2002527118A (ja) 2002-08-27
CN1342208A (zh) 2002-03-27
EP1121470A1 (fr) 2001-08-08
AU1113000A (en) 2000-05-08
KR20010102909A (ko) 2001-11-17

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Legal Events

Date Code Title Description
FZDE Discontinued
FZDE Discontinued

Effective date: 20031015