CA2339349A1 - Enzymes de glutathione-s-transferase issues du mais - Google Patents

Enzymes de glutathione-s-transferase issues du mais Download PDF

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Publication number
CA2339349A1
CA2339349A1 CA002339349A CA2339349A CA2339349A1 CA 2339349 A1 CA2339349 A1 CA 2339349A1 CA 002339349 A CA002339349 A CA 002339349A CA 2339349 A CA2339349 A CA 2339349A CA 2339349 A1 CA2339349 A1 CA 2339349A1
Authority
CA
Canada
Prior art keywords
seq
glutathione
nucleic acid
host cell
leu
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002339349A
Other languages
English (en)
Inventor
Brian Mcgonigle
Daniel P. O'keefe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2339349A1 publication Critical patent/CA2339349A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1088—Glutathione transferase (2.5.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance

Abstract

Cette invention concerne des fragments d'acides nucléiques isolés codant la totalité ou une grande partie des enzymes de glutathione-S-transférase (GST) qui sont issues du maïs et qui jouent un rôle dans la détoxification de composés xénobiotiques dans les plantes et les graines. Cette invention concerne également la construction de gènes chimériques qui codent la totalité ou une grande partie des enzymes de GST issues du maïs, des cellules hôtes transformées à l'aide de ces gènes ainsi que des procédés de production recombinante d'enzymes de GST issues du maïs. Cette invention concerne en outre des procédés de construction de plantes transgéniques possédant des niveaux modifiés d'enzymes de GST, ainsi que des cribles permettant d'identifier des substrats d'enzymes de GST issues du maïs et des inhibiteurs d'enzymes de GST issues du maïs.
CA002339349A 1998-09-30 1998-09-30 Enzymes de glutathione-s-transferase issues du mais Abandoned CA2339349A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1998/020502 WO2000018937A1 (fr) 1998-09-30 1998-09-30 Enzymes de glutathione-s-transferase issues du mais

Publications (1)

Publication Number Publication Date
CA2339349A1 true CA2339349A1 (fr) 2000-04-06

Family

ID=22267980

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002339349A Abandoned CA2339349A1 (fr) 1998-09-30 1998-09-30 Enzymes de glutathione-s-transferase issues du mais

Country Status (4)

Country Link
EP (1) EP1117810A1 (fr)
AU (1) AU1063899A (fr)
CA (1) CA2339349A1 (fr)
WO (1) WO2000018937A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6096504A (en) * 1997-09-05 2000-08-01 E. I. Du Pont Nemours And Company Maize glutathione-S-transferase enzymes
SE0203479D0 (sv) * 2002-11-22 2002-11-22 Bengt Mannervik Novel drug target

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9114259D0 (en) * 1991-07-02 1991-08-21 Ici Plc Plant derived enzyme and dna sequences
DE19501840A1 (de) * 1995-01-23 1996-07-25 Bayer Ag Desoxyribonukleinsäure und ihre Verwendung

Also Published As

Publication number Publication date
EP1117810A1 (fr) 2001-07-25
AU1063899A (en) 2000-04-17
WO2000018937A1 (fr) 2000-04-06

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued