CA2339323A1 - Primer-specific and mispair extension assay for identifying gene variation - Google Patents

Primer-specific and mispair extension assay for identifying gene variation Download PDF

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Publication number
CA2339323A1
CA2339323A1 CA002339323A CA2339323A CA2339323A1 CA 2339323 A1 CA2339323 A1 CA 2339323A1 CA 002339323 A CA002339323 A CA 002339323A CA 2339323 A CA2339323 A CA 2339323A CA 2339323 A1 CA2339323 A1 CA 2339323A1
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CA
Canada
Prior art keywords
primer
specific
extension products
nucleic acid
identifying gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002339323A
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French (fr)
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CA2339323C (en
Inventor
Yu-Wen Hu
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Canadian Blood Services
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Individual
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Publication date
Priority claimed from CA002245039A external-priority patent/CA2245039A1/en
Application filed by Individual filed Critical Individual
Priority to CA002339323A priority Critical patent/CA2339323C/en
Publication of CA2339323A1 publication Critical patent/CA2339323A1/en
Application granted granted Critical
Publication of CA2339323C publication Critical patent/CA2339323C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to primer-specific and mispair extension assays for identifying gene variations, such as in different genotypes or subtypes of a given genotype.
The assay includes extending a nucleic acid sequence from a patient sample with extension products of the primer, characterizing the extension products, and comparing the extension products with known nucleic acid sequences of various genotypes for determining the genotype of the nucleic acid sequence extended. The one or more extension products are terminated in the presence of at least two mispairs within a 2 to 4 base pair range located downstream of the 3' end of the primer. In the assay, at least one primer or the dNTPs is labeled.
CA002339323A 1998-08-13 1999-08-09 Primer-specific and mispair extension assay for identifying gene variation Expired - Fee Related CA2339323C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002339323A CA2339323C (en) 1998-08-13 1999-08-09 Primer-specific and mispair extension assay for identifying gene variation

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CA2,245,039 1998-08-13
CA002245039A CA2245039A1 (en) 1998-08-13 1998-08-13 Primer-specific and mispair extension assay for identifying gene variation
CA002339323A CA2339323C (en) 1998-08-13 1999-08-09 Primer-specific and mispair extension assay for identifying gene variation
PCT/CA1999/000733 WO2000009745A1 (en) 1998-08-13 1999-08-09 Primer-specific and mispair extension assay for identifying gene variation

Publications (2)

Publication Number Publication Date
CA2339323A1 true CA2339323A1 (en) 2000-02-24
CA2339323C CA2339323C (en) 2009-10-27

Family

ID=25680425

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002339323A Expired - Fee Related CA2339323C (en) 1998-08-13 1999-08-09 Primer-specific and mispair extension assay for identifying gene variation

Country Status (1)

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CA (1) CA2339323C (en)

Also Published As

Publication number Publication date
CA2339323C (en) 2009-10-27

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Effective date: 20170809