CA2334215A1 - Lignees cellulaires transformees, exprimant des recepteurs heterologues lies a la proteine g - Google Patents

Lignees cellulaires transformees, exprimant des recepteurs heterologues lies a la proteine g Download PDF

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Publication number
CA2334215A1
CA2334215A1 CA002334215A CA2334215A CA2334215A1 CA 2334215 A1 CA2334215 A1 CA 2334215A1 CA 002334215 A CA002334215 A CA 002334215A CA 2334215 A CA2334215 A CA 2334215A CA 2334215 A1 CA2334215 A1 CA 2334215A1
Authority
CA
Canada
Prior art keywords
receptor
gpc
gene
accordance
heterologous
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002334215A
Other languages
English (en)
Inventor
Helmut Kessmann
Franz Durrenberger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biofocus DPI AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2334215A1 publication Critical patent/CA2334215A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70571—Receptors; Cell surface antigens; Cell surface determinants for neuromediators, e.g. serotonin receptor, dopamine receptor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Endocrinology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Neurology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Selon l'invention, pour mettre en évidence les interactions entre des récepteurs GPC (récepteurs comportant sept domaines transmembranaires qui sont en interaction avec des protéines régulatrices liant le nucléotide guanylique) ou avec des systèmes de transmission de signaux contrôlés par le récepteur GPC et des substances test (ligands, modulateurs) et pour rechercher des substances qui peuvent induire une interaction avec ce type de récepteurs ou de systèmes de transmission de signaux, il est prévu d'utiliser des lignées cellulaires transformées, par exemple de Ustilago maydis. Lesdites lignées cellulaires transformées présentent une voie de transduction de signaux contrôlée par le récepteur GPC, à rétroaction positive et se trouvent transformées, de manière à exprimer un récepteur GPC hétérologue. Les lignées cellulaires contiennent en outre un gène rapporteur dont l'expression peut être détectée selon des techniques de mesure. Ledit gène rapporteur est piloté par un promoteur inductible par stimulation d'un récepteur GPC. Le promoteur inductible par stimulation de récepteur est endogène et le gène rapporteur est endogène ou hétérologue.
CA002334215A 1998-06-05 1999-06-04 Lignees cellulaires transformees, exprimant des recepteurs heterologues lies a la proteine g Abandoned CA2334215A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CH122698 1998-06-05
CH1226/98 1998-06-05
PCT/CH1999/000246 WO1999064567A1 (fr) 1998-06-05 1999-06-04 Lignees cellulaires transformees, exprimant des recepteurs heterologues lies a la proteine g

Publications (1)

Publication Number Publication Date
CA2334215A1 true CA2334215A1 (fr) 1999-12-16

Family

ID=4205446

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002334215A Abandoned CA2334215A1 (fr) 1998-06-05 1999-06-04 Lignees cellulaires transformees, exprimant des recepteurs heterologues lies a la proteine g

Country Status (5)

Country Link
EP (1) EP1084231A1 (fr)
JP (1) JP2002517228A (fr)
AU (1) AU4028199A (fr)
CA (1) CA2334215A1 (fr)
WO (1) WO1999064567A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9631196B2 (en) 2011-06-10 2017-04-25 Temasek Life Sciences Laboratory Limited Genetic manipulation and expression systems for Pucciniomycotina and Ustilaginomycotina subphyla

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002533095A (ja) * 1998-12-22 2002-10-08 アイシス・イノベーション・リミテッド 配列の同定方法
CN113444724B (zh) * 2021-05-10 2023-03-17 西南大学 一种启动子及重组载体和用途

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2628750B1 (fr) * 1988-03-21 1991-11-08 Pasteur Institut Vecteur modifie par une sequence codant pour une sequence d'acides amines contenue dans une proteine de mammifere ayant une activite biologique de recepteur membranaire
US5462856A (en) * 1990-07-19 1995-10-31 Bunsen Rush Laboratories, Inc. Methods for identifying chemicals that act as agonists or antagonists for receptors and other proteins involved in signal transduction via pathways that utilize G-proteins
EP1029915A3 (fr) * 1990-09-13 2004-11-17 Duke University Expression de récepteurs couplés à une protéine G dans une cellule de levure
JP2001508292A (ja) * 1996-12-27 2001-06-26 メルク エンド カンパニー インコーポレーテッド ガラニンレセプターｇａｌｒ２及び該レセプターをコードするヌクレオチド

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9631196B2 (en) 2011-06-10 2017-04-25 Temasek Life Sciences Laboratory Limited Genetic manipulation and expression systems for Pucciniomycotina and Ustilaginomycotina subphyla

Also Published As

Publication number Publication date
WO1999064567A1 (fr) 1999-12-16
JP2002517228A (ja) 2002-06-18
EP1084231A1 (fr) 2001-03-21
AU4028199A (en) 1999-12-30

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Legal Events

Date Code Title Description
FZDE Discontinued