CA2329509A1 - Improved methods and materials for transformation - Google Patents

Improved methods and materials for transformation Download PDF

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Publication number
CA2329509A1
CA2329509A1 CA002329509A CA2329509A CA2329509A1 CA 2329509 A1 CA2329509 A1 CA 2329509A1 CA 002329509 A CA002329509 A CA 002329509A CA 2329509 A CA2329509 A CA 2329509A CA 2329509 A1 CA2329509 A1 CA 2329509A1
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CA
Canada
Prior art keywords
materials
transformation
cell
improved methods
exogenous polynucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002329509A
Other languages
French (fr)
Other versions
CA2329509C (en
Inventor
Lada Rasochova
Thomas L. German
Paul G. Ahlquist
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wisconsin Alumni Research Foundation
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2329509A1 publication Critical patent/CA2329509A1/en
Application granted granted Critical
Publication of CA2329509C publication Critical patent/CA2329509C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Disclosed herein are novel methods and materials directed to transforming a host cell and expressing exogenous RNA therein.
Specifically disclosed are DNA-launching platforms used to introduce a replicating viral segment attached to an exogenous polynucleotide into a cell, whereby the exogenous polynucleotide is expressed in said cell and confers a detectable trait.
CA002329509A 1998-05-22 1999-05-21 Improved methods and materials for transformation Expired - Lifetime CA2329509C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US8652698P 1998-05-22 1998-05-22
US60/086,526 1998-05-22
PCT/US1999/011250 WO1999061597A2 (en) 1998-05-22 1999-05-21 Expression cassette for transformation comprising a modified viral sequence driven by a suitable promoter

Publications (2)

Publication Number Publication Date
CA2329509A1 true CA2329509A1 (en) 1999-12-02
CA2329509C CA2329509C (en) 2009-10-06

Family

ID=22199163

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002329509A Expired - Lifetime CA2329509C (en) 1998-05-22 1999-05-21 Improved methods and materials for transformation

Country Status (7)

Country Link
EP (1) EP1086237A2 (en)
JP (1) JP3959965B2 (en)
AR (1) AR020324A1 (en)
AU (1) AU763096B2 (en)
BR (1) BR9911065A (en)
CA (1) CA2329509C (en)
WO (1) WO1999061597A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8507220B2 (en) 2003-10-01 2013-08-13 Japan Science And Technology Agency Tobacco BY2 cells containing a tomato mosaic virus vector for protein production

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0007231D0 (en) 2000-03-24 2000-05-17 Chiron Spa Modified rna for gene delivery
US6800748B2 (en) * 2001-01-25 2004-10-05 Large Scale Biology Corporation Cytoplasmic inhibition of gene expression and expression of a foreign protein in a monocot plant by a plant viral vector
EP1594956A4 (en) 2003-02-03 2007-08-01 Fraunhofer Usa Inc System for expression of genes in plants

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1288073C (en) * 1985-03-07 1991-08-27 Paul G. Ahlquist Rna transformation vector
WO1990012107A1 (en) * 1989-03-31 1990-10-18 The Salk Institute Biotechnology/Industrial Associates, Inc. Recombinant expression system based on satellite tobacco mosaic virus
JPH04121200A (en) * 1990-09-07 1992-04-22 Nippon Nohyaku Co Ltd Production of polypeptide in plant cell
EP0716148B1 (en) * 1993-09-15 2004-01-02 Chiron Corporation Recombinant alphavirus vectors
GB9703146D0 (en) * 1997-02-14 1997-04-02 Innes John Centre Innov Ltd Methods and means for gene silencing in transgenic plants

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8507220B2 (en) 2003-10-01 2013-08-13 Japan Science And Technology Agency Tobacco BY2 cells containing a tomato mosaic virus vector for protein production

Also Published As

Publication number Publication date
CA2329509C (en) 2009-10-06
WO1999061597A9 (en) 2000-02-24
AU4310199A (en) 1999-12-13
WO1999061597A2 (en) 1999-12-02
AU763096B2 (en) 2003-07-10
BR9911065A (en) 2001-02-06
WO1999061597A3 (en) 2000-03-30
JP3959965B2 (en) 2007-08-15
AR020324A1 (en) 2002-05-08
JP2002516089A (en) 2002-06-04
EP1086237A2 (en) 2001-03-28

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Effective date: 20190521