CA2325819C - Promoteurs, genes et produits geniques de l'enveloppe de la semence - Google Patents

Promoteurs, genes et produits geniques de l'enveloppe de la semence Download PDF

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Publication number
CA2325819C
CA2325819C CA002325819A CA2325819A CA2325819C CA 2325819 C CA2325819 C CA 2325819C CA 002325819 A CA002325819 A CA 002325819A CA 2325819 A CA2325819 A CA 2325819A CA 2325819 C CA2325819 C CA 2325819C
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CA
Canada
Prior art keywords
seed
plant
coat
protein
nucleotide sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002325819A
Other languages
English (en)
Other versions
CA2325819A1 (fr
Inventor
Brian Miki
Mark Gijzen
Shea Miller
Lu-Ann Bowman
Anthea Batchelor
Ming Hu
Kim Boutilier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agriculture and Agri Food Canada AAFC
Original Assignee
Agriculture and Agri Food Canada AAFC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agriculture and Agri Food Canada AAFC filed Critical Agriculture and Agri Food Canada AAFC
Publication of CA2325819A1 publication Critical patent/CA2325819A1/fr
Application granted granted Critical
Publication of CA2325819C publication Critical patent/CA2325819C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0065Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823Reproductive tissue-specific promoters
    • C12N15/8234Seed-specific, e.g. embryo, endosperm

Abstract

Cette invention se rapporte à des séquences génomiques isolées qui sont exprimées différentiellement dans les tissus de l'enveloppe de la semence. Ces ADN sont exprimés dans les cellules du tégument interne, à paroi mince ou épaisse, du parenchyme, de l'endothélium ou du parenchyme palissadique, en sablier ou étoilé de l'enveloppe de la semence. Cette invention se rapporte en outre à des régions promoteurs obtenues à partir de séquences génomiques qui sont exprimées différentiellement dans les tissus de l'enveloppe de la semence, ainsi qu'à leur utilisation pour diriger dans des cellules végétales ou des plantes transformées l'expression des gènes recherchés, spécifique selon l'enveloppe de la semence. Comme exemples de promoteurs on peut citer ceux obtenus à partir du criblage différentiel d'une bibliothèque d'enveloppes de semence et de promoteurs cryptiques produits au moyen du marquage d'ADN-T à l'aide d'un gène marqueur sans promoteur.
CA002325819A 1998-04-13 1999-04-13 Promoteurs, genes et produits geniques de l'enveloppe de la semence Expired - Fee Related CA2325819C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US5909098A 1998-04-13 1998-04-13
US09/059,090 1998-04-13
PCT/CA1999/000293 WO1999053067A2 (fr) 1998-04-13 1999-04-13 Promoteurs, genes et produits geniques de l'enveloppe de la semence

Publications (2)

Publication Number Publication Date
CA2325819A1 CA2325819A1 (fr) 1999-10-21
CA2325819C true CA2325819C (fr) 2008-01-29

Family

ID=22020793

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002325819A Expired - Fee Related CA2325819C (fr) 1998-04-13 1999-04-13 Promoteurs, genes et produits geniques de l'enveloppe de la semence

Country Status (6)

Country Link
EP (1) EP1071782A2 (fr)
JP (1) JP2002511269A (fr)
AU (1) AU3133799A (fr)
BR (1) BR9909630A (fr)
CA (1) CA2325819C (fr)
WO (1) WO1999053067A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999067389A2 (fr) * 1995-05-15 1999-12-29 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada Elements regulateurs cryptiques obtenus a partir de plantes
US6403862B1 (en) 1999-09-24 2002-06-11 Pioneer Hi-Bred International, Inc. Seed-preferred promoter from maize
US6864362B2 (en) * 2000-03-16 2005-03-08 E. I. Du Pont De Nemours And Company Hypoallergenic transgenic soybeans
BRPI0515302A (pt) * 2004-09-14 2008-07-15 Monsanto Technology Llc moléculas promotoras para uso em plantas
US7550579B2 (en) 2005-04-29 2009-06-23 Pioneer Hi-Bred International, Inc. Pericarp-preferred regulatory element
DE112010003162T5 (de) 2009-04-22 2012-08-16 Basf Plant Science Company Gmbh Gesamtsamen-spezifischer Promotor
CN105316316A (zh) * 2014-08-05 2016-02-10 张建福 一种水稻种胚rna的提取方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU639059B2 (en) * 1989-12-22 1993-07-15 E.I. Du Pont De Nemours And Company Site-specific recombination of dna in plant cells
CA2149000C (fr) * 1995-05-09 2007-04-24 Brian Miki Promoteur cache specifique de l'episperme dans le tabac
AU7441996A (en) * 1995-10-27 1997-05-15 Indiana Crop Improvement Association A soybean peroxidase gene family and an assay for detecting soybean peroxidase activity
CA2211018C (fr) * 1996-09-30 2009-03-03 Mark Gijzen Peroxydase et region de regulation de l'adn specifiques du tegument
CA2186833A1 (fr) * 1996-09-30 1998-03-31 Mark Gijzen Adn genomique et peroxydase specifique pour teguments

Also Published As

Publication number Publication date
WO1999053067A2 (fr) 1999-10-21
EP1071782A2 (fr) 2001-01-31
JP2002511269A (ja) 2002-04-16
WO1999053067A8 (fr) 2000-01-20
WO1999053067A3 (fr) 1999-12-09
CA2325819A1 (fr) 1999-10-21
BR9909630A (pt) 2001-09-11
AU3133799A (en) 1999-11-01

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