CA2321930A1 - Production et utilisation d'echantillotheques d'adn normalisees - Google Patents

Production et utilisation d'echantillotheques d'adn normalisees Download PDF

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Publication number
CA2321930A1
CA2321930A1 CA002321930A CA2321930A CA2321930A1 CA 2321930 A1 CA2321930 A1 CA 2321930A1 CA 002321930 A CA002321930 A CA 002321930A CA 2321930 A CA2321930 A CA 2321930A CA 2321930 A1 CA2321930 A1 CA 2321930A1
Authority
CA
Canada
Prior art keywords
genomic dna
dna
library
isolated
population
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002321930A
Other languages
English (en)
Inventor
Jay M. Short
Eric J. Mathur
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/034,724 external-priority patent/US6001574A/en
Application filed by Individual filed Critical Individual
Publication of CA2321930A1 publication Critical patent/CA2321930A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé permettant la constitution d'une échantillothèque normalisée d'ADN génomique à partir d'un échantillon prélevé dans son milieu. Ce procédé consiste d'abord (a) à isoler dans l'échantillon prélevé dans son milieu une population d'ADN génomique. Le procédé consiste ensuite à réaliser l'une au moins des deux opérations consistant (i) à amplifier le nombre d'exemplaires de la population d'ADN ainsi isolée ou (ii) à récupérer une fraction de l'ADN génomique isolé présentant une caractéristique intéressante. Le procédé consiste enfin (c) à normaliser la représentation des divers ADN dans la population d'ADN génomique de façon à constituer une échantillothèque normalisée d'ADN génomique à partir de l'échantillon prélevé dans son milieu. L'invention concerne également une échantillothèque normalisée d'ADN génomique obtenue selon ce procédé à partir d'un échantillon prélevé dans son milieu.
CA002321930A 1998-03-04 1999-03-04 Production et utilisation d'echantillotheques d'adn normalisees Abandoned CA2321930A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/034,724 1998-03-04
US09/034,724 US6001574A (en) 1996-06-18 1998-03-04 Production and use of normalized DNA libraries
PCT/US1999/004917 WO1999045154A1 (fr) 1998-03-04 1999-03-04 Production et utilisation d'echantillotheques d'adn normalisees

Publications (1)

Publication Number Publication Date
CA2321930A1 true CA2321930A1 (fr) 1999-09-10

Family

ID=21878199

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002321930A Abandoned CA2321930A1 (fr) 1998-03-04 1999-03-04 Production et utilisation d'echantillotheques d'adn normalisees

Country Status (6)

Country Link
EP (1) EP1060269A4 (fr)
JP (1) JP2002505121A (fr)
AU (1) AU744699B2 (fr)
CA (1) CA2321930A1 (fr)
MX (1) MXPA00008559A (fr)
WO (1) WO1999045154A1 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8476011B1 (en) 1996-06-18 2013-07-02 Bp Corporation North America Inc. Construction and use of catalogued nucleic acid libraries that contain advantageously adjusted representations of defined components
US5763239A (en) 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6399334B1 (en) * 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
US6605430B1 (en) 1998-08-12 2003-08-12 Maxygen, Inc. DNA shuffling of monooxygenase genes for production of industrial chemicals
JP2002526107A (ja) 1998-10-07 2002-08-20 マキシジェン， インコーポレイテッド マイコトキシンの解毒のための核酸を生成するためのｄｎａシャッフリング
WO2000028018A1 (fr) 1998-11-10 2000-05-18 Maxygen, Inc. Adp-glucose pyrophosphorylase modifiee pour l'amelioration et l'optimisation de phenotypes vegetaux
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7384387B1 (en) 1999-02-11 2008-06-10 Maxygen, Inc. High throughput mass spectrometry
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
FR2808276B1 (fr) 2000-04-26 2004-04-02 Renaud Nalin Procede d'extraction indirecte de l'adn d'organismes non cultivables et adn susceptible d'etre obtenu par ledit procede
CA2411828A1 (fr) 2000-06-23 2002-01-03 Maxygen, Inc. Nouvelles molecules costimulatrices
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
EP1356037B1 (fr) 2001-01-25 2011-03-09 Evolva Ltd. Echantillothèque pour collection de cellules
EP1386966A1 (fr) 2002-07-24 2004-02-04 Libragen Méthode pour l'expresssion de l'ADN environmental dans des cellules hôtes

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5482845A (en) * 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
US5763239A (en) * 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries

Also Published As

Publication number Publication date
WO1999045154A1 (fr) 1999-09-10
EP1060269A1 (fr) 2000-12-20
AU2898199A (en) 1999-09-20
AU744699B2 (en) 2002-02-28
EP1060269A4 (fr) 2004-10-27
MXPA00008559A (es) 2005-06-06
JP2002505121A (ja) 2002-02-19

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