CA2309258A1 - Nutritive media and manufactured seeds comprising same - Google Patents
Nutritive media and manufactured seeds comprising same Download PDFInfo
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- CA2309258A1 CA2309258A1 CA002309258A CA2309258A CA2309258A1 CA 2309258 A1 CA2309258 A1 CA 2309258A1 CA 002309258 A CA002309258 A CA 002309258A CA 2309258 A CA2309258 A CA 2309258A CA 2309258 A1 CA2309258 A1 CA 2309258A1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
- A01H4/006—Encapsulated embryos for plant reproduction, e.g. artificial seeds
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Abstract
Manufactured seeds are disclosed that comprise a unit of totipotent plant tissue and a nutritive medium. The nutritive medium can contain a number of different components selected from the following: a gel solute, charcoal, a carbon source, urea, KNO3, NH4NO3, CuC12, CuSO4, KI, KH2PO4, CaC12, MgSO4, Na2EDTA, FeSO4, ferric citrate, MnSO4, MnCl2, H3BO3, ZnSO4, CoCl2, Na2MoO4, (NH4)2MoO4, thiamine, riboflavin, pyridoxine. HCl, Ca-pantothenate, nicotinic acid, biotin, folic acid, and myo-inositol. The nutritive medium can also include any of various protein amino acids, any of various polyamines, any of various oxygen-absorbing compounds, any of various non-protein amino acids, and/or a smoke suspension.
Claims (47)
1. A manufactured seed, comprising:
(a) a unit of totipotent plant tissue; and (b) a nutritive medium comprising at least 15 components selected from the group consisting of: 0 g/L to 30 g/L gel solute, 5 mM to 200 mM carbon source, 0 mM
to 20 mM
KNO3, 2.5 mM to 5 mM NH4NO3, 0 mM to 0.01 mM KI, 0.01 mM to 20 mM KH2PO4, 0.5 mM
to 5 mM CaCl2, 0 mM to 0.0001 mM CuSO4, 0.1 mM to 10 mM MgSO4, 0 mM to 0.1 mM
Na2EDTA, 0 mM to 0.1 mM FeSO4, 0 mM to 0.1 mM MnSO4, 0.01 mM to 1 mM H3BO3, 0.0001 mM to 0.005 mM ZnSO4, 0 mM to 0.0001 mM CoCl2, 0 mM to 0.001 mM NaiMoO4, 0 mM
to 0.01 mM thiamine.HCl, 0.001 mM to 0.005 mM pyridoxine.HCl, 0.001 mM to 0.02 mM
nicotinic acid, 0.05 mM to 2.5 mM myo-inositol, and 0.002 mM to 0.2 mM L-glycine.
(a) a unit of totipotent plant tissue; and (b) a nutritive medium comprising at least 15 components selected from the group consisting of: 0 g/L to 30 g/L gel solute, 5 mM to 200 mM carbon source, 0 mM
to 20 mM
KNO3, 2.5 mM to 5 mM NH4NO3, 0 mM to 0.01 mM KI, 0.01 mM to 20 mM KH2PO4, 0.5 mM
to 5 mM CaCl2, 0 mM to 0.0001 mM CuSO4, 0.1 mM to 10 mM MgSO4, 0 mM to 0.1 mM
Na2EDTA, 0 mM to 0.1 mM FeSO4, 0 mM to 0.1 mM MnSO4, 0.01 mM to 1 mM H3BO3, 0.0001 mM to 0.005 mM ZnSO4, 0 mM to 0.0001 mM CoCl2, 0 mM to 0.001 mM NaiMoO4, 0 mM
to 0.01 mM thiamine.HCl, 0.001 mM to 0.005 mM pyridoxine.HCl, 0.001 mM to 0.02 mM
nicotinic acid, 0.05 mM to 2.5 mM myo-inositol, and 0.002 mM to 0.2 mM L-glycine.
2. The manufactured seed of claim 1, wherein the nutritive medium further comprises 0 to 5 g/L charcoal.
3. The manufactured seed of claim 1, wherein the carbon source is sucrose.
4. The manufactured seed of claim 1, wherein the nutritive medium further comprises an amino acid selected from the group consisting of: L-asparagine, L-glutamine, L-lysine, L-serine, L-proline, L-arginine, L-valine, L-alanine, L-cysteine, L-leucine, L-tyrosine, L-threonine, L-phenylalanine, L-histidine, L-tryptophan, L-isoleucine, L-methionine, and mixtures thereof.
5. The manufactured seed of claim 1, wherein the nutritive medium further comprises a smoke suspension.
6. The manufactured seed of claim 1, wherein the nutritive medium further comprises urea.
7. The manufactured seed of claim 1, wherein the nutritive medium further comprises a non-protein amino acid.
8. The manufactured seed of claim 7, wherein the non-protein amino acid is ornithine.
9. The manufactured seed of claim 1, wherein the nutritive medium further comprises a polyamine selected from the group consisting of putrescine, spermidine, spermine, and mixtures thereof.
10. The manufactured seed of claim 1, wherein the nutritive medium further comprises riboflavin.
11. The manufactured seed of claim 1, wherein the nutritive medium further comprises Ca-pantothenate.
12. The manufactured seed of claim 1, wherein the nutritive medium further comprises biotin.
13. The manufactured seed of claim 1, wherein the nutritive medium further comprises folic acid.
14. The manufactured seed of claim 1, wherein the nutritive medium further comprises an oxygen-absorbing substance.
15. The manufactured seed of claim 1, wherein the nutritive medium comprises:
58.43 mM sucrose, 11.57 mM KNO3, 2.577 mM NH4NO3, 0.0025 mM KI, 0.625 mM KH2PO4, 1.133 mM CaCl2, 3.2x10-5 mM CuSO4, 0.367 mM MgSO4, 0.05 mM Na2EDTA, 0.027 mM FeSO4, 0.045 mM MnSO4, 0.05 mM H3BO3, 8.4x10-3 mM ZnSO4, 2.9x10-5 mM CoCl2, 4.4x10-4 mM
Na2MoO4, 0.003 mM thiamine.HCl, 0.0024 mM pyridoxine.HCl, 0.0041 mM nicotinic acid, 0.555 mM myo-inositol, and 0.027 mM L-glycine.
58.43 mM sucrose, 11.57 mM KNO3, 2.577 mM NH4NO3, 0.0025 mM KI, 0.625 mM KH2PO4, 1.133 mM CaCl2, 3.2x10-5 mM CuSO4, 0.367 mM MgSO4, 0.05 mM Na2EDTA, 0.027 mM FeSO4, 0.045 mM MnSO4, 0.05 mM H3BO3, 8.4x10-3 mM ZnSO4, 2.9x10-5 mM CoCl2, 4.4x10-4 mM
Na2MoO4, 0.003 mM thiamine.HCl, 0.0024 mM pyridoxine.HCl, 0.0041 mM nicotinic acid, 0.555 mM myo-inositol, and 0.027 mM L-glycine.
16. A manufactured seed, comprising:
(a) a unit of totipotent plant tissue; and (b) a nutritive medium comprising at least 15 components selected from the group consisting of: 0 g/L to 30 g/L gel solute, 0 g/L to 5 g/L charcoal, 5 mM to 200 mM carbon source, 0 mM to 30 mM urea, 0 mM to 20 mM KNO3, 2.5 mM to 5 mM NH4NO3, 0 mM to 0.01 mM KI, 0.01 mM to 20 mM KH2PO4, 0.5 mM to 5 mM CaCl2, 0.1 mM to 10 mM MgSO4, 0 mM
to 0.1 mM Na2EDTA, 0 mM to 0.1 mM FeSO4, 0 mM to 0.5 mM ferric citrate, 0 mM
to 0.1 mM
MnSO4, 0 mM to 0.1 mM MnCl2, 0.01 mM to 1 mM H3BO3, 0.0001 mM to 0.005 mM
ZnSO4, 0 mM to 0.0001 mM CoCl2, 0 mM to 0.0001 mM CuSO4, 0 mM to 0.005 mM CuCl2, 0 mM
to 0.001 mM Na2MoO4, 0 mM to 0.001 mM (NH4)2MoO4, 0 mM to 0.01 mM thiamine.HCl, 0 mM to 0.001 mM riboflavin, 0.001 mM to 0.005 mM pyridoxine.HCl, 0 mM to 0.04 mM
Ca-pantothenate, 0.001 mM to 0.02 mM nicotinic acid, 0 mM to 1.0x10-5 mM
biotin, 0 mM to 0.001 mM folic acid, 0.05 mM to 2.5 mM myo-inositol; and at least 1 component selected from the group consisting of: 0 mM to 2 mM L-asparagine, 0 mM to 6 mM L-glutamine, 0 mM
to 2 mM
L-lysine, 0 mM to 2 mM L-serine, 0 mM to 2 mM L-proline, 0 mM to 8 mM L-arginine, 0 mM to 2 mM L-valine, 0 mM to 2 mM L-alanine, 0 mM to 2 mM L-cysteine, 0 mM to 2 mM L-leucine, 0 mM to 2 mM L-tyrosine, 0 mM to 2 mM L-threonine, 0 mM to 2 mM L-phenylalanine, 0 mM to 2 mM L-histidine, 0.002 mM to 0.2 mM L-glycine, 0 mM to 2 mM L-tryptophan, 0 mM to 2 mM
L-isoleucine, and 0 mM to 2 mM L-methionine.
(a) a unit of totipotent plant tissue; and (b) a nutritive medium comprising at least 15 components selected from the group consisting of: 0 g/L to 30 g/L gel solute, 0 g/L to 5 g/L charcoal, 5 mM to 200 mM carbon source, 0 mM to 30 mM urea, 0 mM to 20 mM KNO3, 2.5 mM to 5 mM NH4NO3, 0 mM to 0.01 mM KI, 0.01 mM to 20 mM KH2PO4, 0.5 mM to 5 mM CaCl2, 0.1 mM to 10 mM MgSO4, 0 mM
to 0.1 mM Na2EDTA, 0 mM to 0.1 mM FeSO4, 0 mM to 0.5 mM ferric citrate, 0 mM
to 0.1 mM
MnSO4, 0 mM to 0.1 mM MnCl2, 0.01 mM to 1 mM H3BO3, 0.0001 mM to 0.005 mM
ZnSO4, 0 mM to 0.0001 mM CoCl2, 0 mM to 0.0001 mM CuSO4, 0 mM to 0.005 mM CuCl2, 0 mM
to 0.001 mM Na2MoO4, 0 mM to 0.001 mM (NH4)2MoO4, 0 mM to 0.01 mM thiamine.HCl, 0 mM to 0.001 mM riboflavin, 0.001 mM to 0.005 mM pyridoxine.HCl, 0 mM to 0.04 mM
Ca-pantothenate, 0.001 mM to 0.02 mM nicotinic acid, 0 mM to 1.0x10-5 mM
biotin, 0 mM to 0.001 mM folic acid, 0.05 mM to 2.5 mM myo-inositol; and at least 1 component selected from the group consisting of: 0 mM to 2 mM L-asparagine, 0 mM to 6 mM L-glutamine, 0 mM
to 2 mM
L-lysine, 0 mM to 2 mM L-serine, 0 mM to 2 mM L-proline, 0 mM to 8 mM L-arginine, 0 mM to 2 mM L-valine, 0 mM to 2 mM L-alanine, 0 mM to 2 mM L-cysteine, 0 mM to 2 mM L-leucine, 0 mM to 2 mM L-tyrosine, 0 mM to 2 mM L-threonine, 0 mM to 2 mM L-phenylalanine, 0 mM to 2 mM L-histidine, 0.002 mM to 0.2 mM L-glycine, 0 mM to 2 mM L-tryptophan, 0 mM to 2 mM
L-isoleucine, and 0 mM to 2 mM L-methionine.
17. The manufactured seed of claim 16, wherein the nutritive medium further comprises 0 to 5 g/L charcoal.
18. The manufactured seed of claim 16, wherein the nutritive medium further comprises a non-protein amino acid.
19. The manufactured seed of claim 18, wherein the non-protein amino acid is ornithine.
20. The manufactured seed of claim 16, wherein the carbon source is sucrose.
21. The manufactured seed of claim 16, wherein the nutritive medium further comprises a smoke suspension.
22. The manufactured seed of claim 16, wherein the nutritive medium further comprises a polyamine selected from the group consisting of putrescine, spermidine, spermine, and mixtures thereof.
23. The manufactured seed of claim 16, wherein the nutritive medium further comprises an oxygen-absorbing substance.
24. A manufactured seed, comprising:
(a) a unit of totipotent plant tissue; and (h) a nutritive medium comprising 0 g/L to 30 g/L gel solute, 2.5 g/L
charcoal, 146.07 mM sucrose, 13.32 mM urea, 3.74 mM NH4NO3, 13.23 mM KH2PO4, 1.54 mM CaCl2, 1.98 mM
MgSO4, 0.245 mM ferric citrate, 0.162 mM H3BO3, 0.0023 mM CuCl2, 0.0003 mM
(NH4)2MoO4, 0.0012 mM thiamine.HCl, 0.0003 mM riboflavin, 0.0012 mM pyridoxine.HCl, 0.001 mM
Ca-pantothenate, 0.0081 mM nicotinic acid, 4.09x10-6 mM biotin, 0.0003 mM
folic acid, 0.555 mM
myo-inositol, 0.8076 mM L-asparagine, 1.8248 mM L-glutamine, 0.1624 mM L-lysine, 0.7613 mM L-serine, 0.463 mM L-proline, 1.0471 mM L-arginine, 0.455 mM L-valine, 0.5983 mM
L-alanine, 0.6099 mM L-cysteine, 0.6099 mM L-leucine, 0.2942 mM L-tyrosine, 0.2241 mM
L-threonine, 0.3227 mM L-phenylalanine, 0.1721 mM L-histidine, 0.71 mM L-glycine, 0.1307 mM
L-tryptophan, 0.2036 mM L-isoleucine, and 0.1789 mM L-methionine.
(a) a unit of totipotent plant tissue; and (h) a nutritive medium comprising 0 g/L to 30 g/L gel solute, 2.5 g/L
charcoal, 146.07 mM sucrose, 13.32 mM urea, 3.74 mM NH4NO3, 13.23 mM KH2PO4, 1.54 mM CaCl2, 1.98 mM
MgSO4, 0.245 mM ferric citrate, 0.162 mM H3BO3, 0.0023 mM CuCl2, 0.0003 mM
(NH4)2MoO4, 0.0012 mM thiamine.HCl, 0.0003 mM riboflavin, 0.0012 mM pyridoxine.HCl, 0.001 mM
Ca-pantothenate, 0.0081 mM nicotinic acid, 4.09x10-6 mM biotin, 0.0003 mM
folic acid, 0.555 mM
myo-inositol, 0.8076 mM L-asparagine, 1.8248 mM L-glutamine, 0.1624 mM L-lysine, 0.7613 mM L-serine, 0.463 mM L-proline, 1.0471 mM L-arginine, 0.455 mM L-valine, 0.5983 mM
L-alanine, 0.6099 mM L-cysteine, 0.6099 mM L-leucine, 0.2942 mM L-tyrosine, 0.2241 mM
L-threonine, 0.3227 mM L-phenylalanine, 0.1721 mM L-histidine, 0.71 mM L-glycine, 0.1307 mM
L-tryptophan, 0.2036 mM L-isoleucine, and 0.1789 mM L-methionine.
25. A manufactured seed, comprising:
(a) a unit of totipotent plant tissue; and (b) a nutritive medium comprising at least 15 components selected from the group consisting of: 10 g/L to 25 g/L gel solute, 25 mM to 175 mM carbon source, 10 mM to 15 mM
KNO3, 2 mM to 4 mM NH4NO3, 0.002 mM to 0.005 mM KI, 0.05 mM to 18 mM KH2PO4, 1 mM
to 3 mM CaCl2, 0.2 mM to 3 mM MgSO4, 0.01 mM to 0.8 mM Na2EDTA, 0.01 mM to 0.05 mM
FeSO4, 0.02 mM to 0.5 mM MnSO4, 0.1 mM to 0.5 mM H3BO3, 0.0003 mM to 0.004 mM
ZnSO4, 1x10-5 mM to 1x10-4 mM CoCl2, 1x10-5 mM to 1x10-4 mM CuSO4, 1x10-4 mM to 0.001 mM
Na2MoO4, 0.001 mM to 0.005 mM thiamine.HCI, 0.001 mM to 0.004 mM
pyridoxine.HCl, 0.001 mM to 0.01 mM nicotinic acid, 0.1 mM to 1 mM myo-inositol, and 0.01 mM to 0.1 mM
L-glycine.
(a) a unit of totipotent plant tissue; and (b) a nutritive medium comprising at least 15 components selected from the group consisting of: 10 g/L to 25 g/L gel solute, 25 mM to 175 mM carbon source, 10 mM to 15 mM
KNO3, 2 mM to 4 mM NH4NO3, 0.002 mM to 0.005 mM KI, 0.05 mM to 18 mM KH2PO4, 1 mM
to 3 mM CaCl2, 0.2 mM to 3 mM MgSO4, 0.01 mM to 0.8 mM Na2EDTA, 0.01 mM to 0.05 mM
FeSO4, 0.02 mM to 0.5 mM MnSO4, 0.1 mM to 0.5 mM H3BO3, 0.0003 mM to 0.004 mM
ZnSO4, 1x10-5 mM to 1x10-4 mM CoCl2, 1x10-5 mM to 1x10-4 mM CuSO4, 1x10-4 mM to 0.001 mM
Na2MoO4, 0.001 mM to 0.005 mM thiamine.HCI, 0.001 mM to 0.004 mM
pyridoxine.HCl, 0.001 mM to 0.01 mM nicotinic acid, 0.1 mM to 1 mM myo-inositol, and 0.01 mM to 0.1 mM
L-glycine.
26. The manufactured seed of claim 25, wherein the nutritive medium further comprises 0 to 5 g/L charcoal.
27. The manufactured seed of claim 25, wherein the nutritive medium further comprises a non-protein amino acid.
28. The manufactured seed of claim 27, wherein the non-protein amino acid is ornithine.
29. The manufactured seed of claim 25, wherein the carbon source is sucrose.
30. The manufactured seed of claim 25, wherein the nutritive medium further comprises a smoke suspension.
31. The manufactured seed of claim 25, wherein the nutritive medium further comprises a polyamine selected from the group consisting of putrescine, spermidine, spermine, and mixtures thereof.
32. The manufactured seed of claim 25, wherein the nutritive medium further comprises riboflavin.
33. The manufactured seed of claim 25, wherein the nutritive medium further comprises Ca-pantothenate.
34. The manufactured seed of claim 25, wherein the nutritive medium further comprises biotin.
35. The manufactured seed of claim 25, wherein the nutritive medium further comprises folic acid.
36. The manufactured seed of claim 25, wherein the nutritive medium further comprises an oxygen-absorbing substance.
37. A manufactured seed comprising:
(a) a unit of totipotent plant tissue; and (b) a nutritive medium comprising at least 15 components selected from a group consisting of: 10 g/L to 25 g/L gel solute, 25 mM to 175 mM carbon source, 10 mM to 15 mM
KNO3, 2 mM to 4 mM NH4NO3, 0.002 mM to 0.005 mM KI, 0.05 mM to 18 mM KH2PO4, 1 mM to 3 mM
CaCl2, 0.2 mM to 3 mM MgSO4, 0.01 mM to 0.8 mM Na2EDTA, 0.01 mM to 0.05 mM
FeSO4, 0.02 mM to 0.5 mM MnSO4, 0.1 mM to 0.5 mM H3BO3, 0.0003 mM to 0.004 mM ZnSO4, 1x10-5 mM to 1x10-4 mM CoCl2, 1x10-5 mM to 1x10-4 mM CuSO4, 1x10-4 mM to 0.001 mM
Na2MoO4, 0.001 mM to 0.005 mM thiamine.HCI, 0.001 mM to 0.004 mM pyridoxine.HCl, 0.001 mM to 0.01 mM nicotinic acid, 0.1 mM to 1 mM myo-inositol, 5 mM to 20 mM urea, 0.1 mM to 0.4 mM
ferric citrate, 0.01 mM to 0.05 mM MnCl2, 0.001 mM to 0.005 mM CuCl2. 1x10-4 mM to 1x10-3 mM (NH4)2MoO4, 1x10-4 mM to 1x10-3 mM riboflavin, 0.0005 mM to 0.015 mM Ca-pantothenate, 1x10-7 mM to 1x10-5 mM biotin, 1x10-4 mM to 1x10-3 mM folic acid; and at least one component selected from the group consisting of: 0.01 mM to 0.1 mM L-glycine, 0.01 mM to 1 mM
L-asparagine, 0.01mM to 3 mM L-glutamine, 0.01 mM to 1 mM L-lysine, 0.01 mM to 1 mM
L-serine, 0.01 mM to 1 mM L-proline, 0.01 mM to 4 mM L-arginine, 0.01 mM to 1 mM L-valine, 0.01 mM to 1 mM L-alanine, 0.01 mM to 1 mM L-cysteine, 0.01 mM to 1 mM L-leucine, 0.01 mM to 1 mM L-tyrosine, 0.01 mM to 1 mM L-threonine, 0.01 mM to 1 mM L-phenylalanine, 0.01 mM to 1 mM L-histidine, 0.01 mM to 0.1 mM L-glycine, 0.01 mM to 1 mM L-tryptophan, 0.01 mM to 1 mM L-isoleucine, and 0.01 mM to 1 mM L-methionine.
(a) a unit of totipotent plant tissue; and (b) a nutritive medium comprising at least 15 components selected from a group consisting of: 10 g/L to 25 g/L gel solute, 25 mM to 175 mM carbon source, 10 mM to 15 mM
KNO3, 2 mM to 4 mM NH4NO3, 0.002 mM to 0.005 mM KI, 0.05 mM to 18 mM KH2PO4, 1 mM to 3 mM
CaCl2, 0.2 mM to 3 mM MgSO4, 0.01 mM to 0.8 mM Na2EDTA, 0.01 mM to 0.05 mM
FeSO4, 0.02 mM to 0.5 mM MnSO4, 0.1 mM to 0.5 mM H3BO3, 0.0003 mM to 0.004 mM ZnSO4, 1x10-5 mM to 1x10-4 mM CoCl2, 1x10-5 mM to 1x10-4 mM CuSO4, 1x10-4 mM to 0.001 mM
Na2MoO4, 0.001 mM to 0.005 mM thiamine.HCI, 0.001 mM to 0.004 mM pyridoxine.HCl, 0.001 mM to 0.01 mM nicotinic acid, 0.1 mM to 1 mM myo-inositol, 5 mM to 20 mM urea, 0.1 mM to 0.4 mM
ferric citrate, 0.01 mM to 0.05 mM MnCl2, 0.001 mM to 0.005 mM CuCl2. 1x10-4 mM to 1x10-3 mM (NH4)2MoO4, 1x10-4 mM to 1x10-3 mM riboflavin, 0.0005 mM to 0.015 mM Ca-pantothenate, 1x10-7 mM to 1x10-5 mM biotin, 1x10-4 mM to 1x10-3 mM folic acid; and at least one component selected from the group consisting of: 0.01 mM to 0.1 mM L-glycine, 0.01 mM to 1 mM
L-asparagine, 0.01mM to 3 mM L-glutamine, 0.01 mM to 1 mM L-lysine, 0.01 mM to 1 mM
L-serine, 0.01 mM to 1 mM L-proline, 0.01 mM to 4 mM L-arginine, 0.01 mM to 1 mM L-valine, 0.01 mM to 1 mM L-alanine, 0.01 mM to 1 mM L-cysteine, 0.01 mM to 1 mM L-leucine, 0.01 mM to 1 mM L-tyrosine, 0.01 mM to 1 mM L-threonine, 0.01 mM to 1 mM L-phenylalanine, 0.01 mM to 1 mM L-histidine, 0.01 mM to 0.1 mM L-glycine, 0.01 mM to 1 mM L-tryptophan, 0.01 mM to 1 mM L-isoleucine, and 0.01 mM to 1 mM L-methionine.
38. The manufactured seed of claim 37, wherein the nutritive medium further comprises 0 to 5 g/L charcoal.
39. The manufactured seed of claim 37, wherein the nutritive medium further comprises a non-protein amino acid.
40. The manufactured seed of claim 39, wherein the non-protein amino acid is ornithine.
41. The manufactured seed of claim 37, wherein the carbon source is sucrose.
42. The manufactured seed of claim 37, wherein the nutritive medium further comprises a smoke suspension.
43. The manufactured seed of claim 37, wherein the nutritive medium further comprises a polyamine selected from the group consisting of putrescine, spermidine, spermine, and mixtures thereof.
44. A nutritive medium, comprising: 58.43 mM sucrose, 11.57 mM KNO3, 2.577 mM
NH4NO3, 0.0025 mM KI, 0.625 mM KH2PO4, 1.133 mM CaCI2, 0.367 mM MgSO4, 0.05 mM
Na2EDTA, 0.027 mM FeSO4, 0.045 mM MnSO4, 0.05 mM H3BO3, 8.4x10-3 mM ZnSO4, 3.2x10-5 mM CuSO4, 2.9x10-5 mM CoCl2, 4.4x10-4 mM Na2MoO4, 0.003 mM thiamine.HCl, 0.0024 mM
pyridoxine.HCl, 0.0041 mM nicotinic acid, 0.555 mM myo-inositol, and 0.027 mM
L-glycine.
NH4NO3, 0.0025 mM KI, 0.625 mM KH2PO4, 1.133 mM CaCI2, 0.367 mM MgSO4, 0.05 mM
Na2EDTA, 0.027 mM FeSO4, 0.045 mM MnSO4, 0.05 mM H3BO3, 8.4x10-3 mM ZnSO4, 3.2x10-5 mM CuSO4, 2.9x10-5 mM CoCl2, 4.4x10-4 mM Na2MoO4, 0.003 mM thiamine.HCl, 0.0024 mM
pyridoxine.HCl, 0.0041 mM nicotinic acid, 0.555 mM myo-inositol, and 0.027 mM
L-glycine.
45. A nutritive medium, comprising: 2.5 g/L charcoal, 146.07 mM sucrose, 13.32 mM
urea, 3.74 mM NH4NO3, 1.98 mM MgSO4, 0.019 MnCl2, 0.0016 ZnSO4, 13.23 mM
KH2PO4, 1.54 mM CaCl2, 0.245 mM ferric citrate, 0.162 mM H3BO3, 0.0023 mM CuCl2, 0.0003 mM
(NH4)2MoO4, 0.0012 mM thiamine.HCl, 0.0003 mM riboflavin, 0.0012 mM
pyridoxine.HCl, 0.0010 mM Ca-pantothenate, 0.0081 mM nicotinic acid, 4.09x10-6 mM biotin, 0.0003 mM folic acid, 0.555 mM myo-inositol, 0.8076 mM L-asparagine, 1.8248 mM L-glutamine, 0.1624 mM
L-lysine, 0.7613 mM L-serine, 0.4630 mM L-proline, 1.0471 mM L-arginine, 0.4550 mM L-valine, 0.5983 mM L-alanine, 0.6099 mM L-cysteine, 0.6099 mM L-leucine, 0.2942 mM L-tyrosine, 0.2241 mM L-threonine, 0.3227 mM L-phenylalanine, 0.1721 mM L-histidine, 0.7100 mM
L-glycine, 0.1307 mM L-tryptophan, 0.2036 mM L-isoleucine, and 0.1789 mM L-methionine.
urea, 3.74 mM NH4NO3, 1.98 mM MgSO4, 0.019 MnCl2, 0.0016 ZnSO4, 13.23 mM
KH2PO4, 1.54 mM CaCl2, 0.245 mM ferric citrate, 0.162 mM H3BO3, 0.0023 mM CuCl2, 0.0003 mM
(NH4)2MoO4, 0.0012 mM thiamine.HCl, 0.0003 mM riboflavin, 0.0012 mM
pyridoxine.HCl, 0.0010 mM Ca-pantothenate, 0.0081 mM nicotinic acid, 4.09x10-6 mM biotin, 0.0003 mM folic acid, 0.555 mM myo-inositol, 0.8076 mM L-asparagine, 1.8248 mM L-glutamine, 0.1624 mM
L-lysine, 0.7613 mM L-serine, 0.4630 mM L-proline, 1.0471 mM L-arginine, 0.4550 mM L-valine, 0.5983 mM L-alanine, 0.6099 mM L-cysteine, 0.6099 mM L-leucine, 0.2942 mM L-tyrosine, 0.2241 mM L-threonine, 0.3227 mM L-phenylalanine, 0.1721 mM L-histidine, 0.7100 mM
L-glycine, 0.1307 mM L-tryptophan, 0.2036 mM L-isoleucine, and 0.1789 mM L-methionine.
46. A method for germinating a unit of totipotent plant tissue, comprising:
(a) providing a unit of totipotent plant tissue;
(b) providing a nutritive medium comprising at least 15 components selected from the group consisting of: 0g/L to 30 g/L gel solute, 5 mM to 200 mM carbon source, 0 mM to 20mM
KNO3, 2.5 mM to 5 mM NH4NO3, 0 mM to 0.01 mM KI, 0.01 mM to 20 mM KH2PO4, 0.5 mM
to 5 mM CaCl2, 0mM to 0.0001 mM CuSO4, 0.1 mM to 10 mM MgSO4, 0 mM to 0.1 mM
Na2EDTA, 0 mM to 0.1 mM FeSO4, 0 mM to 0.1 mM MnSO4, 0.01 mM to 1 mM H3BO3, 0.0001 mM to 0.005 mM ZnSO4, 0 mM to 0.0001 mM CoCl2, 0 mM to 0.001 mM Na2MoO4, 0 mM
to 0.01 mM thiamine.HCl, 0.001 mM to 0.005 mM pyridoxine.HCl, 0.001 mM to 0.02 mM
nicotinic acid, 0.05 mM to 2.5 mM myo-inositol, and 0.002 mM to 0.2 mM L-glycine;
(c) placing the totipotent plant tissue in functional contact with the nutritive medium in a manufactured seed; and (d) placing the manufactured seed in an environment conducive for plant growth so as to allow the plant tissue to grow and germinate from the manufactured seed.
(a) providing a unit of totipotent plant tissue;
(b) providing a nutritive medium comprising at least 15 components selected from the group consisting of: 0g/L to 30 g/L gel solute, 5 mM to 200 mM carbon source, 0 mM to 20mM
KNO3, 2.5 mM to 5 mM NH4NO3, 0 mM to 0.01 mM KI, 0.01 mM to 20 mM KH2PO4, 0.5 mM
to 5 mM CaCl2, 0mM to 0.0001 mM CuSO4, 0.1 mM to 10 mM MgSO4, 0 mM to 0.1 mM
Na2EDTA, 0 mM to 0.1 mM FeSO4, 0 mM to 0.1 mM MnSO4, 0.01 mM to 1 mM H3BO3, 0.0001 mM to 0.005 mM ZnSO4, 0 mM to 0.0001 mM CoCl2, 0 mM to 0.001 mM Na2MoO4, 0 mM
to 0.01 mM thiamine.HCl, 0.001 mM to 0.005 mM pyridoxine.HCl, 0.001 mM to 0.02 mM
nicotinic acid, 0.05 mM to 2.5 mM myo-inositol, and 0.002 mM to 0.2 mM L-glycine;
(c) placing the totipotent plant tissue in functional contact with the nutritive medium in a manufactured seed; and (d) placing the manufactured seed in an environment conducive for plant growth so as to allow the plant tissue to grow and germinate from the manufactured seed.
47. A method for germinating a unit of totipotent plant tissue, comprising:
(a) providing a unit of totipotent plant tissue;
(b) providing a nutritive medium comprising at least 15 components selected from the group consisting of: 0 g/L to 30 g/L gel solute, 0 g/L to 5 g/L charcoal, 5 mM
to 200 mM carbon source, 0 mM to 30 mM urea, 0 mM to 20 mM KNO3, 2.5 mM to 5 mM NH4NO3, 0 mM to 0.01 mM KI, 0.01 mM to 20 mM KH2PO4, 0.5 mM to 5 mM CaCl2, 0.1 mM to 10 mM MgSO4, 0 mM
to 0.1 mM Na2EDTA, 0 mM to 0.1 mM FeSO4, 0 mM to 0.5 mM ferric citrate, 0 mM
to 0.1 mM
MnSO4, 0 mM to 0.1 mM MnCl2, 0.01 mM to 1 mM H3BO3, 0.0001 mM to 0.005 mM
ZnSO4, 0 mM to 0.0001 mM CoCl2, 0 mM to 0.0001 mM CuSO4, 0 mM to 0.005 mM CuCl2, 0 mM
to 0.001 mM Na2MoO4, 0 mM to 0.001 mM (NH4)2MoO4, 0 mM to 0.01 mM thiamine.HCl, 0 mM to 0.001 mM riboflavin, 0.001 mM to 0.005 mM pyridoxine.HCl, 0 mM to 0.04 mM
Ca-pantothenate, 0.001 mM to 0.02 mM nicotinic acid, 0 mM to 1.0x10-5 mM
biotin, 0 mM to 0.001 mM folic acid, 0.05 mM to 2.5 mM myo-inositol; and at least one component selected from the group consisting of: 0 mM to 2 mM L-asparagine, 0 mM to 6 mM L-glutamine, 0 mM
to 2 mM
L-lysine, 0 mM to 2 mM L-serine, 0 mM to 2 mM L-proline, 0 mM to 8 mM L-arginine, 0 mM to 2 mM L-valine, 0 mM to 2 mM L-alanine, 0 mM to 2 mM L-cysteine, 0 mM to 2 mM L-leucine, 0 mM to 2 mM L-tyrosine, 0 mM to 2 mM L-threonine, 0 mM to 2 mM L-phenylalanine, 0 mM to 2 mM L-histidine, 0.002 mM to 0.2 mM L-glycine, 0 mM to 2 mM L-tryptophan, 0 mM to 2 mM
L-isoleucine, and 0 mM to 2 mM L-methionine;
(c) placing the totipotent plant tissue in functional contact with the nutritive medium in a manufactured seed; and (d) placing the manufactured seed in an environment conducive for plant growth so as to allow the plant tissue to grow and germinate from the manufactured seed.
(a) providing a unit of totipotent plant tissue;
(b) providing a nutritive medium comprising at least 15 components selected from the group consisting of: 0 g/L to 30 g/L gel solute, 0 g/L to 5 g/L charcoal, 5 mM
to 200 mM carbon source, 0 mM to 30 mM urea, 0 mM to 20 mM KNO3, 2.5 mM to 5 mM NH4NO3, 0 mM to 0.01 mM KI, 0.01 mM to 20 mM KH2PO4, 0.5 mM to 5 mM CaCl2, 0.1 mM to 10 mM MgSO4, 0 mM
to 0.1 mM Na2EDTA, 0 mM to 0.1 mM FeSO4, 0 mM to 0.5 mM ferric citrate, 0 mM
to 0.1 mM
MnSO4, 0 mM to 0.1 mM MnCl2, 0.01 mM to 1 mM H3BO3, 0.0001 mM to 0.005 mM
ZnSO4, 0 mM to 0.0001 mM CoCl2, 0 mM to 0.0001 mM CuSO4, 0 mM to 0.005 mM CuCl2, 0 mM
to 0.001 mM Na2MoO4, 0 mM to 0.001 mM (NH4)2MoO4, 0 mM to 0.01 mM thiamine.HCl, 0 mM to 0.001 mM riboflavin, 0.001 mM to 0.005 mM pyridoxine.HCl, 0 mM to 0.04 mM
Ca-pantothenate, 0.001 mM to 0.02 mM nicotinic acid, 0 mM to 1.0x10-5 mM
biotin, 0 mM to 0.001 mM folic acid, 0.05 mM to 2.5 mM myo-inositol; and at least one component selected from the group consisting of: 0 mM to 2 mM L-asparagine, 0 mM to 6 mM L-glutamine, 0 mM
to 2 mM
L-lysine, 0 mM to 2 mM L-serine, 0 mM to 2 mM L-proline, 0 mM to 8 mM L-arginine, 0 mM to 2 mM L-valine, 0 mM to 2 mM L-alanine, 0 mM to 2 mM L-cysteine, 0 mM to 2 mM L-leucine, 0 mM to 2 mM L-tyrosine, 0 mM to 2 mM L-threonine, 0 mM to 2 mM L-phenylalanine, 0 mM to 2 mM L-histidine, 0.002 mM to 0.2 mM L-glycine, 0 mM to 2 mM L-tryptophan, 0 mM to 2 mM
L-isoleucine, and 0 mM to 2 mM L-methionine;
(c) placing the totipotent plant tissue in functional contact with the nutritive medium in a manufactured seed; and (d) placing the manufactured seed in an environment conducive for plant growth so as to allow the plant tissue to grow and germinate from the manufactured seed.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US6623297P | 1997-11-20 | 1997-11-20 | |
| US60/066,232 | 1997-11-20 | ||
| PCT/US1998/024820 WO1999026470A1 (en) | 1997-11-20 | 1998-11-20 | Nutritive media and manufactured seeds comprising same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2309258A1 true CA2309258A1 (en) | 1999-06-03 |
| CA2309258C CA2309258C (en) | 2012-05-01 |
Family
ID=22068160
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2309258A Expired - Fee Related CA2309258C (en) | 1997-11-20 | 1998-11-20 | Nutritive media and manufactured seeds comprising same |
Country Status (4)
| Country | Link |
|---|---|
| AU (1) | AU731857B2 (en) |
| CA (1) | CA2309258C (en) |
| NZ (1) | NZ505099A (en) |
| WO (1) | WO1999026470A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006094400A1 (en) * | 2005-03-10 | 2006-09-14 | Cellfor Inc. | Aerated liquid priming of conifer somatic germinants |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8466086B2 (en) | 1997-11-20 | 2013-06-18 | Weyerhaeuser Nr Company | Nutritive media and manufactured seeds comprising same |
| DE10011998A1 (en) * | 2000-03-11 | 2001-09-20 | Forschungszentrum Juelich Gmbh | A new growth promoting active substance combination contains L-serine, L-asparagine and/or L-glutamine provides a growth medium for cells, particularly human or mammalian cells |
| DE10130215B4 (en) | 2001-06-22 | 2007-08-30 | Hottinger Baldwin Messtechnik Gmbh | Measuring sensor with connected data memory |
| US7228658B2 (en) | 2003-08-27 | 2007-06-12 | Weyerhaeuser Company | Method of attaching an end seal to manufactured seeds |
| CA2486289C (en) | 2003-11-25 | 2008-01-08 | Weyerhaeuser Company | Combination end seal and restraint |
| US7555865B2 (en) | 2003-11-25 | 2009-07-07 | Weyerhaeuser Nr Company | Method and system of manufacturing artificial seed coats |
| US7795029B2 (en) * | 2003-12-04 | 2010-09-14 | Cellfor Inc. | Method of ex vitro sowing, germination, growth and conversion of plant somatic embryos or germinants |
| US7356965B2 (en) | 2003-12-11 | 2008-04-15 | Weyerhaeuser Co. | Multi-embryo manufactured seed |
| US7591287B2 (en) | 2003-12-18 | 2009-09-22 | Weyerhaeuser Nr Company | System and method for filling a seedcoat with a liquid to a selected level |
| US7568309B2 (en) | 2004-06-30 | 2009-08-04 | Weyerhaeuser Nr Company | Method and system for producing manufactured seeds |
| US7547488B2 (en) | 2004-12-15 | 2009-06-16 | Weyerhaeuser Nr Company | Oriented strand board panel having improved strand alignment and a method for making the same |
| US7654037B2 (en) | 2005-06-30 | 2010-02-02 | Weyerhaeuser Nr Company | Method to improve plant somatic embryo germination from manufactured seed |
| EP1974014B1 (en) | 2006-01-04 | 2017-04-19 | Baxalta Incorporated | Oligopeptide-free cell culture media |
| AT503565B1 (en) * | 2006-06-16 | 2007-11-15 | Jhs Privatstiftung | PROCESS FOR THE PRODUCTION OF ORGANICALLY BOUND VITAMIN B |
| CA2713626C (en) | 2009-09-30 | 2013-11-19 | Weyerhaeuser Nr Company | Method to improve germination of embryos from manufactured seed |
| CA2713838C (en) | 2009-09-30 | 2014-12-02 | Weyerhaeuser Nr Company | Methods of preparing nutritive media for growth and/or germination of plant embryos |
| CA2713903C (en) | 2009-09-30 | 2014-07-29 | Weyerhaeuser Nr Company | Nutritive media for use in manufactured seeds |
| RU2449525C2 (en) * | 2010-04-15 | 2012-05-10 | Анатолий Александрович Астахов | Agent for presowing seeds treatment, mostly sunflower (versions) |
| CN115380662B (en) * | 2022-10-27 | 2023-03-21 | 中国医学科学院药用植物研究所 | Seed coating method for improving drought resistance of isatis roots in seedling stage |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5427593A (en) * | 1990-10-26 | 1995-06-27 | Weyerhaeuser Company | Analogs of botanic seed |
| US5771632A (en) * | 1996-09-23 | 1998-06-30 | Liu; Sijiu | Artificial seed with a powder structure for anti-contamination |
-
1998
- 1998-11-20 CA CA2309258A patent/CA2309258C/en not_active Expired - Fee Related
- 1998-11-20 WO PCT/US1998/024820 patent/WO1999026470A1/en active IP Right Grant
- 1998-11-20 NZ NZ505099A patent/NZ505099A/en not_active IP Right Cessation
- 1998-11-20 AU AU15314/99A patent/AU731857B2/en not_active Ceased
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006094400A1 (en) * | 2005-03-10 | 2006-09-14 | Cellfor Inc. | Aerated liquid priming of conifer somatic germinants |
| US7923249B2 (en) | 2005-03-10 | 2011-04-12 | Cellfor Inc. | Aerated liquid priming of conifer somatic germinants |
Also Published As
| Publication number | Publication date |
|---|---|
| AU1531499A (en) | 1999-06-15 |
| CA2309258C (en) | 2012-05-01 |
| AU731857B2 (en) | 2001-04-05 |
| WO1999026470A1 (en) | 1999-06-03 |
| NZ505099A (en) | 2002-03-01 |
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