CA2297350A1 - Wide range cleaning and disinfecting preparation - Google Patents
Wide range cleaning and disinfecting preparation Download PDFInfo
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- CA2297350A1 CA2297350A1 CA 2297350 CA2297350A CA2297350A1 CA 2297350 A1 CA2297350 A1 CA 2297350A1 CA 2297350 CA2297350 CA 2297350 CA 2297350 A CA2297350 A CA 2297350A CA 2297350 A1 CA2297350 A1 CA 2297350A1
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Abstract
The present invention relates to a preparation for use in hospital and laboratory environments as a means for prevention of disease transmission effective for a wide range of pathogenic microorganisms. The inventive preparation is also applicable as general sanitation and household disinfecting means. More specifically the invention relates to a novel preparation comprising cleaning and bleaching agents that effectively eliminates organic matter and acts against mycobacteria and a wide number of viruses such as polio, coxsackie, herpes, influenza, Hepatitis B, HIV and others while at the same time destroying a variety of pathogenic bacteria such as E.coli, Pseudomonas fluorescens, Shigella dysenteriae, Clostridium botulinum, Staphylococcus aureus, and having an enhanced fungicidal effect.
Description
Background of the Invention Effective cleaning and disinfecting of working areas in medical, public and household environments is of major importance in the prevention of disease transmission.
With recent increases in levels of concern about transmission risks of a number of microbial agents, much attention has been paid in recent years to the testing of widely accepted cleaning agents for actual effectiveness in respect of specific types of pathogenic microorganisms where working conditions do not facilitate prolonged txeatxnent under high temperatures.
It has been shown that several known bleaching agents for surface use in hospital and laboratory environments are effective against the HIV virus as well as Hepatitis B virus.
Thus for example quarteraary ammonium salts, sodium hypochlorite and a variety of alcohols and phenols were found to have such anti viral activity in a research by Resnick et al., JAMA
April 11, 1986 -Vol. 255, no. 14.
It is also known that microorganisms vary widely in their resistance to chemical germicides.
Thus bacterial spores, mycobacteria and to a relatively lesser degree tubercle bacilli, small or non lipid viruses and vegetative fungi as well as asexual fungal spores, vegetative bacteria and medium sized or lipid containing viruses may withstand treatment with disinfectants under routine cleaning procedures.
Cleaning and bleaching procedures are of crucial importance in the disinfection of medical instruments as prior physical cleaning is a sine qua non for the effectiveness of any sterilization procedure.
It is of similar importance to ensure that disease does not spread via human contact with public or household surfaces and objects contaminated by pathogenic viruses, bacteria, mycobacteria or fungi. Untreated fruit and vegetables are yet another source of infection.
There is thus a widely recognized need for, and it would be highly advantageous to have, a bleaching-disinfecting preparation for hospital and laboratory surfaces and for cleaning medical instruments with a wide range germicidal action that combines fungicidal activity with anti viral, mycobactericidal and bactericidal effectiveness and that is applicable in a suitable form such as diluted liquid, ointment, dry powder or aerosol for general cleaning and disinfecting in the household or in public places.
Summary of the Invention According to the present invention there is provided a wide range germicidal action disinfecting preparation consisting of a solution of of 7-13% sodium bromide w/v, 3-6%
peracetic acid w/v, 2-4% phthalic acid dipotassium salt 98°l° w/v, 3-5% acetanilide w/v, 12-L6% isopropanol v/v, 64% water v/v, and optionally 1 % soap v/v for the disinfection of hospital and laboratory surfaces and medical environments. The inventive preparation significantly increases the prevention of disease transmission by cleaning and disinfecting of hospital and laboratory surfaces and equipment.
In accordance with another aspect of the invention the preparation may contain a detergent that enhances the disinfecting action of the preparation by removing organic particles the presence of which is known to sigiuficantly hilder decontamination.
W accordance with a further aspect of the invention the preparation may be diluted for use in the cleaning a.nd disinfecting of household equipment, furluslung and other surfaces as well as cleaning and disinfection of fruit and vegetables, and preservative for flowers.
In accordance with yet another advantage of the present invention the preparation may be applied as an external disinfectant for human patients.
The inventive preparation may be prepared and used in liquid solutions, as aerosol, as humidifier in cleansilg tissues, as ointment with a suitable emulsifier or in dry powder formulation and it may be used on its own or in admixture with other disinfectants or as an addition to soaps or detergents.
In accordance with a further aspect of the invention a suitable catalytic agent may be added to activate the formulation and enhance the bleaching and disiifecting effect.
The inventive preparation acts effectively agaiist mycobacteria and a wide number of viruses such as polio, coxsackie, herpes, influenza, Hepatitis B, HIV and others while at the same tine destroying a variety of pathogenic bacteria such as E.coli, Pseudomonas fluorescens, Shigella dysenteriae, Clostridium botulinum, Staphylococcus aureus, and having an enhanced fungicidal 4~~~/~f Vii W.
Detailed Description of the Invention The present invention relates to a preparation for bleaching and disinfecting hospital and laboratory surfaces and medical equipment, that is also applicable for household sanitation and skin disinfection at suitable low concentrations, consisting of a solution of 7-13% sodium bromide w/v, 3-6% peracetic acid w/v, 2-4% phthalic acid dipotassium salt 9$%
w/v, 3-5%
acetanilide w/v, 12-16% isopropanol v/v, 64% water v/v, to which a detergent is optionally added.
The preparation according to the invention effectively disinfects and prevents disease transmission for a wide range of microorganisms including a wide number of viruses such as polio, coxsackie, herpes, influenza, Hepatitis B, HIV and others while at the same rime destroying mycobacteria and a variety of pathogenic bacteria such as E.coli, Pseudomonas fluorescens, Salmonella, Shigella dysenteriae, Clostridium botulinum, Staphylococcus aureus, and having an enhanced fungicidal effect.
In accordance with another embodiment of the invention, the preparation also contains detergents that enable the elimination of organic matter from work surfaces and equipment.
The inventive preparation has the additional advantage that elimination of organic matter highly facilitates disinfection whereby the combination has a synergetic effect.
The preparation is suitable for household and skin disinfection use at a dilution of 1:10.
In accordance with the invention the disinfectant preparation may be prepared as a liquid aqueous or inert solution or it may be formulated as an ointment with a suitable emulsifier or it may be prepared for use in an aerosol container or as a liquid in saturated cleansing tissues or contained within a solid soap preparation.
It is envisaged that the inventive preparation may be applied as a pesticide at high concentrations and it may be added to water as a preservative for cut flowers at low, household concentrations.
The inventive preparation may comprise a catalytic component to enhance the bleaching and germicidal action.
It will be appreciated by those versed in the art that the invention has been described hereinabove in respect of a preferred embodiment and many variations, modifications and improvements may be made that still remain within the ambit of the invention as described and claimed.
Laboratory testing Efficacy testing of the preparation in accordance with the invention was carried out by the appropriate AOAC official methods as shown below:
I. MYCOBACTE~CTDAL, TESTS
Materials and Methode The tests were earned out with a formulation consisting of 7% sodium bromide w/v, 3%
peracetic acid w/v, 3% phthalic acid, dipotassium salt 98% w/v, 4% acetanilide w/v, 15%
isopropanol v/v, 64% water v/v, 1 % soap v/v.
Mycobacterium terrae ATCC 15755: M. terrae was in 7H9 broth (Difco) containing glycerol but no antibiotics. The bacterial suspension was centrifuged at 2,500 rpm for 15 minutes and the pellet resuspended to give approximately 2.5 x 10g cells/mL (No. 8 McFarland Standard).
Sterile Saline: The diluent and rinse used for the mycobactericidal test was 0.85% sterile saline.
Organic Load: Fetal bovine serum was used as the organic load. It was added to the test microbial suspension at a final concentration of 5%.
The test involved drying a microbial suspension on a hard surface carrier and covering the dried inoculum with the use dilution of the disinfectant for the specified contact time and temperature.
At the end of the contact time, a diluent/rinse was used for recovering the inoculum from the carrier and the eluent was passed through a membrane filter (0.45 pm pore diameter) to capture the test organism. The filters were then placed on plates with an appropriate recovery medium and incubated to allow viable organisms to form visible colonies. The numbers of colony forming units were recorded and the level of inactivation of the test organism was calculated by comparison to controls.
The testing used glass vials as the hard surface carriers. The organism used for the mycobactericidal tests was Mycobacterium terrae suspended in fetal bovine serum at a final nnnnan+r~tinn n~ C°/ Txxrn nHcaminol fnrmWofinsxa xx~a~~
to°.°~tvd ai a vv~ntact iu v° of ~~v m~~'ute.°~
vvuvvaaaa uwvai v~ r i v. i rr v vxavuuvux ivmaauaua.avu.~ rr v and 30 minutes. After rinsing and filtering, the filter was placed on 7H11 agar and incubated at 37C for a total of 4 weeks. The plates were monitored and counted (CFU) at weekly intervals.
Control carriers were used in the same manner as test carriers except sterile saline was applied tn ~tn ~rio~ jir~vvultia~ii lx'istead of fine vh°vii'ii~c''~1 formulator. The number of teJt Va111eJ 1'~~U.J ~.
w mu iws Three control carries were incorporated in each test. The results are reported as loglo reductions in viability with reference to the mean titre on the control carriers. For the product to be considered mycobactericidal, it was expected to reduce the viable titre of all the test organisms by a minimum of 6 loglo (at least 1 million fold) under the conditions of this test.
Carrier Inoculation: Carrier inoculation involved placing 10 ~.L (between 10~
to 10g colony forming units) of the test microbial suspension on the inside bottom surface of a glass vial and allowing the inoculum to dry.
Ra r~rl tr The results for this phase of the tests are shown in Table 1.
Table 1. The germicidal activity of the Formulation against li~ycobacterium terrae:
i Experiment Contact time Average I Average I Loge Reduction i I I CFU~'Control CFU~'Test I
i i i I Carrier i i I Carrier I
1 1 i 20 ( 4.0x106 i 0 i >6.6 I
1 2 ( 30 i 4.4x10 i 0 i >6.6 I
, , i , i 3 i 20 1 4.3x106 1 0 i >6.6 i C''n~r~~l"e;n~.
~/V iV11iJ1V 1.
The formulation in its diluted form was proved as mycobactericidal T_T_, FTTNC''TT_C''TT~AT. TFCT$
The tests were carried out with a formulation consisting of 7% sodium bromide w/v, 3%
peracetic acid w/v, 3% phthalic acid, dipotassium salt 98% w/v, 4% acetanilide w/v, 15%
isopropanol v/v, 64% water v/v, 1% soap v/v wherein two of the ingredients have a ~~nnrat,trotinn lnixrnr ~hae~ tha in~rcanti~ra fnr~»~~t~n,~, VV11VV11416~1.1V11 1V ~~ V1 1(1.. 411V 111 ~ V11 1 ~ V 1V1 11 V 1.
Trichophyton mentagrophytes was used as the test organisem.
Rdatcariale Rr TllFcathnr~e 1~1(1.W1141.1J W 1~1V411V~4U
Sterile Saline: The diluent and rinse used for the fungicidal test was 0.85%
sterile saline.
Organic Load: Fetal bovine serum was used as the organic load. It was added to the test fungal suspension at a final concentration of 5%.
The ~,3uantitative Carrier Test:. The test involves drying a microbal suspension on a hard surface carrier and covering the dried inoculum with the use-dilution of the disinfectant for the specified contact time and temperature, At the end of the contact time, a diluent / rinse was used for specified contact time and temperature. At the end of the contact time, a diluent / rinse was used for recoveritlg the inoculum from the carrier and the eluent was passed through a membrane filter {0.45 ~Im pore diameter) to capture the test organism. The filters were then placed on plates with an appropriate recovery medium and itlcubated to allow viable organisms to form visible colonies. The numbers of colony forming units were recorded and the level of inactivation of the test orgatusm was calculated iti comparison to controls.
The testing used glass vials as the hard surface carriers.
The organism used for the fungicidal tests was a cordial suspension of Trichophyton mentagrophytes suspended in fetal bovine serum at a final concentration of S%.
One chemical formulation was tested at a contact tune of 10 minutes. After rinsing a.nd filtering, the filter was placed on Mycobiotic agar and incubated at 28 ° c for a total of 10 days. The plates were monitored and counted (CFU) daily. Control carriers were used in the same manner as test carries except sterile saline was applied to the dried inoculum instead of the chemical formulation. The number of test carriers was 5. Three control carriers were incorporated in each test. The results are reported as loglo reductions in viability in reference with the control carries.
For the product to be considered fungicidal, it was expected to reduce the viable titre of all the test organisms by a minimum of 5 logio under the conditions of this test.
Carrier W ovulation: Carrier inoculation involved placing 10 L (between 105 -106 colony forming units) of the test fungal suspension on the inside bottom surface of a glass vial and allowing the inoculum to dry.
The results of the fungicidal testing are shown in Table 2.
Table 2. The fungicidal activity of the formulation against Trichophyton mentagrophytes Experiment Date of Expt Contact Time Average GFU/Test Loge, I
~ ~ (minutes) ~ CFU/Control~ Average ~ Reduction Carner I Carrier I I I Sep 29, 1997 I I0 I 7.3xI05 I 0 I 5.9 I
I 2 I Oct 2, 1997 I IO I 5.9xI05 I (3 I 5.8 I
(-''nn~lncinn The formulation in its diluted form proved fungicidal.
rrr wr~r turner ~r~crrc 111. ~ uw vlir~ ray 1 a.iV 1 V
Poliovirus type 1 (Sabin~ was used for this test i11 accordance with the Canadian General C+.~nr~nrrlo Rn~~d tv~t f~~' ~'~ar~alcldvs.
V l.~,ylui4 <iJ tJ
Ma Pri al c Rr MPt nrl c wt___~ ~3.._...
TY1P a~7~ent ~d d~l~vni used for the vii~uc~dul test YvU.s ERSS.
111V V (.L1 A formulation consisting of 7% sodium bromide w/v, 3% peracetic acid w/v, 3%
phthalic acid, dipotassium salt 98% wlv, 4% acetanilide wlv, 15% isopropanol v/v, 64% water v/v, 1 % soap vlv (formula # 1 ) and a preferred embodiment of the inventive formulation, consisting of a solution of 10% sodium bromide w/v, 5% peracetic acid w/v, 3% phthalic acid dipotassiutn salt 98% w/v, 4% acetaiulide w/v, 15% isopropanol v/v, 64% water v/v, and 1% soap (formula #2) were used as virucidal agents in these tests.
Polio Sabin 1 virus already available in the laboratory was grown on Vero cells. Cells were maintained by passage in minimum essential medium (MEM) from Gibco BRL Cat #12360-038 with the appropriate supplements and 10% fetal bovine serum (FBS) in 25cm2 flasks in a C02 incubator at 37°C. Two hundred microliter of the viral suspension was inoculated onto a confluent monolayer washed three times with 10 mL of Earls Balanced Salt Solution (EBSS) from Gibco BRL Cat #450-1 100EL to remove serum. The virus was allowed to spread by gentle rocking of the flask. The flask was kept at 37°C for 50 min. to allow for virus adsorption.
Supplemented MEM without serum was added and incubated for 24 hours. When 75%
Cytopathic effect (CPE) was observed. The virus was separated from the cells by three rapid freeze thawing followed by gentle centrifugation at 1500 rpm for 15 min. The supernatant which contained the virus was dispensed in aliquots of 200~L and stored at -80°C. The virus titre was determined using a plaque assay method.
Organic Load: Fetal bovine serum was used as the organic load in this study.
It was added to the test virus suspension at a final concentration of 5%.
The testing used flat stainless steel discs as the hard surface carriers. The organism used for the virucide tests was polio type 1 (Sabin) suspended in fetal bovine serum at a final concentration of 5%. Carrier inoculation involved placing 10~L (between 10g-lO6 plaque forming units) of the testvirus suspension on the center of each stainless steel carrier and allowing the inoculum to dry for 1 hour.
The dried itioculum was covered with the use dilution of the disinfectant for the specified contact tune and temperature. At the end of the contact time, EBSS was used as neutralizer/eluent for recovering the reaction mixture from the carrier. The eluent was serially diluted and plaque assay done to obtain titre of virus remaining.
Each formulation ~~las tested at a contact time of 10 minutes. Control carriers were used in the same manner as test carriers except sterile EBSS was applied to the dried inoculum instead of the chemical formulation. The number of test carriers was 5. Three control carriers were incorporated ul each. The results are reported as loglo reductions in viability in reference with the control carriers. For the formulation to be considered virucidal, it was expected to reduce the viable titre of all the test organisms by a minimum of 4 loglo under the conditions of this test.
Results The results of virucidal tests are showTn in Tables 3 and ~. Table ~ shows that formula #1 was not virucidal, it had little or no activity against poliovirus at a contact time of 10 minutes at 20=1°C.
Table 3. The germicidal activity of the formulation #1 against Polio type 1 (Sabin) ExperimentDate of Contact Average PFU/ControlPFU/Test AverageLogy Expt Time Carrier Garner I Reduction minutes ~
I
1 i Sep 9, 199710 1.X105 1.8X104 j 1 ~
I 2 ~ Sep 22, 10 7.2XI04 I.9XI04 j 1997 I I I I I I I
The polivirus was retested with formula #2 which increased the concentration of two of the ingredients. Formula #2 is a preferred embodiment of the inventive formula that consists of a solution of 10% sodium bromide w/v, 5% peracetic acid w/v, 3% phthalic acid dipotassium salt 98% w/v, 4% acetanilide w/v, 15% isopropanol v/v, 64% water v/v, and 1% soap v/v. The test methods used were identical of those describcd above. Formula #2 ~.~.ras able to reduce the ~~:~us titre by more than 4 loglo (Table 4).
Table 4. The germicidal activity of the inventive formulation against Poliovirus type 1 (Sabin) ExperimentDate of Contact Average PFU/ControlPFU/Test AverageLogo I I Expt Time Carrier I Carrier I Reduction I minutes I
I
1 I Nov 24, 10 I 6.8X104 0 4.83 I
I
2 ~ Nov 25, ~ 10 i 1.25X10 I 0 i 5.10 I
1~~
7,4 f'nn~l_ycin_n_ Formula #2 proved virucidal.
With recent increases in levels of concern about transmission risks of a number of microbial agents, much attention has been paid in recent years to the testing of widely accepted cleaning agents for actual effectiveness in respect of specific types of pathogenic microorganisms where working conditions do not facilitate prolonged txeatxnent under high temperatures.
It has been shown that several known bleaching agents for surface use in hospital and laboratory environments are effective against the HIV virus as well as Hepatitis B virus.
Thus for example quarteraary ammonium salts, sodium hypochlorite and a variety of alcohols and phenols were found to have such anti viral activity in a research by Resnick et al., JAMA
April 11, 1986 -Vol. 255, no. 14.
It is also known that microorganisms vary widely in their resistance to chemical germicides.
Thus bacterial spores, mycobacteria and to a relatively lesser degree tubercle bacilli, small or non lipid viruses and vegetative fungi as well as asexual fungal spores, vegetative bacteria and medium sized or lipid containing viruses may withstand treatment with disinfectants under routine cleaning procedures.
Cleaning and bleaching procedures are of crucial importance in the disinfection of medical instruments as prior physical cleaning is a sine qua non for the effectiveness of any sterilization procedure.
It is of similar importance to ensure that disease does not spread via human contact with public or household surfaces and objects contaminated by pathogenic viruses, bacteria, mycobacteria or fungi. Untreated fruit and vegetables are yet another source of infection.
There is thus a widely recognized need for, and it would be highly advantageous to have, a bleaching-disinfecting preparation for hospital and laboratory surfaces and for cleaning medical instruments with a wide range germicidal action that combines fungicidal activity with anti viral, mycobactericidal and bactericidal effectiveness and that is applicable in a suitable form such as diluted liquid, ointment, dry powder or aerosol for general cleaning and disinfecting in the household or in public places.
Summary of the Invention According to the present invention there is provided a wide range germicidal action disinfecting preparation consisting of a solution of of 7-13% sodium bromide w/v, 3-6%
peracetic acid w/v, 2-4% phthalic acid dipotassium salt 98°l° w/v, 3-5% acetanilide w/v, 12-L6% isopropanol v/v, 64% water v/v, and optionally 1 % soap v/v for the disinfection of hospital and laboratory surfaces and medical environments. The inventive preparation significantly increases the prevention of disease transmission by cleaning and disinfecting of hospital and laboratory surfaces and equipment.
In accordance with another aspect of the invention the preparation may contain a detergent that enhances the disinfecting action of the preparation by removing organic particles the presence of which is known to sigiuficantly hilder decontamination.
W accordance with a further aspect of the invention the preparation may be diluted for use in the cleaning a.nd disinfecting of household equipment, furluslung and other surfaces as well as cleaning and disinfection of fruit and vegetables, and preservative for flowers.
In accordance with yet another advantage of the present invention the preparation may be applied as an external disinfectant for human patients.
The inventive preparation may be prepared and used in liquid solutions, as aerosol, as humidifier in cleansilg tissues, as ointment with a suitable emulsifier or in dry powder formulation and it may be used on its own or in admixture with other disinfectants or as an addition to soaps or detergents.
In accordance with a further aspect of the invention a suitable catalytic agent may be added to activate the formulation and enhance the bleaching and disiifecting effect.
The inventive preparation acts effectively agaiist mycobacteria and a wide number of viruses such as polio, coxsackie, herpes, influenza, Hepatitis B, HIV and others while at the same tine destroying a variety of pathogenic bacteria such as E.coli, Pseudomonas fluorescens, Shigella dysenteriae, Clostridium botulinum, Staphylococcus aureus, and having an enhanced fungicidal 4~~~/~f Vii W.
Detailed Description of the Invention The present invention relates to a preparation for bleaching and disinfecting hospital and laboratory surfaces and medical equipment, that is also applicable for household sanitation and skin disinfection at suitable low concentrations, consisting of a solution of 7-13% sodium bromide w/v, 3-6% peracetic acid w/v, 2-4% phthalic acid dipotassium salt 9$%
w/v, 3-5%
acetanilide w/v, 12-16% isopropanol v/v, 64% water v/v, to which a detergent is optionally added.
The preparation according to the invention effectively disinfects and prevents disease transmission for a wide range of microorganisms including a wide number of viruses such as polio, coxsackie, herpes, influenza, Hepatitis B, HIV and others while at the same rime destroying mycobacteria and a variety of pathogenic bacteria such as E.coli, Pseudomonas fluorescens, Salmonella, Shigella dysenteriae, Clostridium botulinum, Staphylococcus aureus, and having an enhanced fungicidal effect.
In accordance with another embodiment of the invention, the preparation also contains detergents that enable the elimination of organic matter from work surfaces and equipment.
The inventive preparation has the additional advantage that elimination of organic matter highly facilitates disinfection whereby the combination has a synergetic effect.
The preparation is suitable for household and skin disinfection use at a dilution of 1:10.
In accordance with the invention the disinfectant preparation may be prepared as a liquid aqueous or inert solution or it may be formulated as an ointment with a suitable emulsifier or it may be prepared for use in an aerosol container or as a liquid in saturated cleansing tissues or contained within a solid soap preparation.
It is envisaged that the inventive preparation may be applied as a pesticide at high concentrations and it may be added to water as a preservative for cut flowers at low, household concentrations.
The inventive preparation may comprise a catalytic component to enhance the bleaching and germicidal action.
It will be appreciated by those versed in the art that the invention has been described hereinabove in respect of a preferred embodiment and many variations, modifications and improvements may be made that still remain within the ambit of the invention as described and claimed.
Laboratory testing Efficacy testing of the preparation in accordance with the invention was carried out by the appropriate AOAC official methods as shown below:
I. MYCOBACTE~CTDAL, TESTS
Materials and Methode The tests were earned out with a formulation consisting of 7% sodium bromide w/v, 3%
peracetic acid w/v, 3% phthalic acid, dipotassium salt 98% w/v, 4% acetanilide w/v, 15%
isopropanol v/v, 64% water v/v, 1 % soap v/v.
Mycobacterium terrae ATCC 15755: M. terrae was in 7H9 broth (Difco) containing glycerol but no antibiotics. The bacterial suspension was centrifuged at 2,500 rpm for 15 minutes and the pellet resuspended to give approximately 2.5 x 10g cells/mL (No. 8 McFarland Standard).
Sterile Saline: The diluent and rinse used for the mycobactericidal test was 0.85% sterile saline.
Organic Load: Fetal bovine serum was used as the organic load. It was added to the test microbial suspension at a final concentration of 5%.
The test involved drying a microbial suspension on a hard surface carrier and covering the dried inoculum with the use dilution of the disinfectant for the specified contact time and temperature.
At the end of the contact time, a diluent/rinse was used for recovering the inoculum from the carrier and the eluent was passed through a membrane filter (0.45 pm pore diameter) to capture the test organism. The filters were then placed on plates with an appropriate recovery medium and incubated to allow viable organisms to form visible colonies. The numbers of colony forming units were recorded and the level of inactivation of the test organism was calculated by comparison to controls.
The testing used glass vials as the hard surface carriers. The organism used for the mycobactericidal tests was Mycobacterium terrae suspended in fetal bovine serum at a final nnnnan+r~tinn n~ C°/ Txxrn nHcaminol fnrmWofinsxa xx~a~~
to°.°~tvd ai a vv~ntact iu v° of ~~v m~~'ute.°~
vvuvvaaaa uwvai v~ r i v. i rr v vxavuuvux ivmaauaua.avu.~ rr v and 30 minutes. After rinsing and filtering, the filter was placed on 7H11 agar and incubated at 37C for a total of 4 weeks. The plates were monitored and counted (CFU) at weekly intervals.
Control carriers were used in the same manner as test carriers except sterile saline was applied tn ~tn ~rio~ jir~vvultia~ii lx'istead of fine vh°vii'ii~c''~1 formulator. The number of teJt Va111eJ 1'~~U.J ~.
w mu iws Three control carries were incorporated in each test. The results are reported as loglo reductions in viability with reference to the mean titre on the control carriers. For the product to be considered mycobactericidal, it was expected to reduce the viable titre of all the test organisms by a minimum of 6 loglo (at least 1 million fold) under the conditions of this test.
Carrier Inoculation: Carrier inoculation involved placing 10 ~.L (between 10~
to 10g colony forming units) of the test microbial suspension on the inside bottom surface of a glass vial and allowing the inoculum to dry.
Ra r~rl tr The results for this phase of the tests are shown in Table 1.
Table 1. The germicidal activity of the Formulation against li~ycobacterium terrae:
i Experiment Contact time Average I Average I Loge Reduction i I I CFU~'Control CFU~'Test I
i i i I Carrier i i I Carrier I
1 1 i 20 ( 4.0x106 i 0 i >6.6 I
1 2 ( 30 i 4.4x10 i 0 i >6.6 I
, , i , i 3 i 20 1 4.3x106 1 0 i >6.6 i C''n~r~~l"e;n~.
~/V iV11iJ1V 1.
The formulation in its diluted form was proved as mycobactericidal T_T_, FTTNC''TT_C''TT~AT. TFCT$
The tests were carried out with a formulation consisting of 7% sodium bromide w/v, 3%
peracetic acid w/v, 3% phthalic acid, dipotassium salt 98% w/v, 4% acetanilide w/v, 15%
isopropanol v/v, 64% water v/v, 1% soap v/v wherein two of the ingredients have a ~~nnrat,trotinn lnixrnr ~hae~ tha in~rcanti~ra fnr~»~~t~n,~, VV11VV11416~1.1V11 1V ~~ V1 1(1.. 411V 111 ~ V11 1 ~ V 1V1 11 V 1.
Trichophyton mentagrophytes was used as the test organisem.
Rdatcariale Rr TllFcathnr~e 1~1(1.W1141.1J W 1~1V411V~4U
Sterile Saline: The diluent and rinse used for the fungicidal test was 0.85%
sterile saline.
Organic Load: Fetal bovine serum was used as the organic load. It was added to the test fungal suspension at a final concentration of 5%.
The ~,3uantitative Carrier Test:. The test involves drying a microbal suspension on a hard surface carrier and covering the dried inoculum with the use-dilution of the disinfectant for the specified contact time and temperature, At the end of the contact time, a diluent / rinse was used for specified contact time and temperature. At the end of the contact time, a diluent / rinse was used for recoveritlg the inoculum from the carrier and the eluent was passed through a membrane filter {0.45 ~Im pore diameter) to capture the test organism. The filters were then placed on plates with an appropriate recovery medium and itlcubated to allow viable organisms to form visible colonies. The numbers of colony forming units were recorded and the level of inactivation of the test orgatusm was calculated iti comparison to controls.
The testing used glass vials as the hard surface carriers.
The organism used for the fungicidal tests was a cordial suspension of Trichophyton mentagrophytes suspended in fetal bovine serum at a final concentration of S%.
One chemical formulation was tested at a contact tune of 10 minutes. After rinsing a.nd filtering, the filter was placed on Mycobiotic agar and incubated at 28 ° c for a total of 10 days. The plates were monitored and counted (CFU) daily. Control carriers were used in the same manner as test carries except sterile saline was applied to the dried inoculum instead of the chemical formulation. The number of test carriers was 5. Three control carriers were incorporated in each test. The results are reported as loglo reductions in viability in reference with the control carries.
For the product to be considered fungicidal, it was expected to reduce the viable titre of all the test organisms by a minimum of 5 logio under the conditions of this test.
Carrier W ovulation: Carrier inoculation involved placing 10 L (between 105 -106 colony forming units) of the test fungal suspension on the inside bottom surface of a glass vial and allowing the inoculum to dry.
The results of the fungicidal testing are shown in Table 2.
Table 2. The fungicidal activity of the formulation against Trichophyton mentagrophytes Experiment Date of Expt Contact Time Average GFU/Test Loge, I
~ ~ (minutes) ~ CFU/Control~ Average ~ Reduction Carner I Carrier I I I Sep 29, 1997 I I0 I 7.3xI05 I 0 I 5.9 I
I 2 I Oct 2, 1997 I IO I 5.9xI05 I (3 I 5.8 I
(-''nn~lncinn The formulation in its diluted form proved fungicidal.
rrr wr~r turner ~r~crrc 111. ~ uw vlir~ ray 1 a.iV 1 V
Poliovirus type 1 (Sabin~ was used for this test i11 accordance with the Canadian General C+.~nr~nrrlo Rn~~d tv~t f~~' ~'~ar~alcldvs.
V l.~,ylui4 <iJ tJ
Ma Pri al c Rr MPt nrl c wt___~ ~3.._...
TY1P a~7~ent ~d d~l~vni used for the vii~uc~dul test YvU.s ERSS.
111V V (.L1 A formulation consisting of 7% sodium bromide w/v, 3% peracetic acid w/v, 3%
phthalic acid, dipotassium salt 98% wlv, 4% acetanilide wlv, 15% isopropanol v/v, 64% water v/v, 1 % soap vlv (formula # 1 ) and a preferred embodiment of the inventive formulation, consisting of a solution of 10% sodium bromide w/v, 5% peracetic acid w/v, 3% phthalic acid dipotassiutn salt 98% w/v, 4% acetaiulide w/v, 15% isopropanol v/v, 64% water v/v, and 1% soap (formula #2) were used as virucidal agents in these tests.
Polio Sabin 1 virus already available in the laboratory was grown on Vero cells. Cells were maintained by passage in minimum essential medium (MEM) from Gibco BRL Cat #12360-038 with the appropriate supplements and 10% fetal bovine serum (FBS) in 25cm2 flasks in a C02 incubator at 37°C. Two hundred microliter of the viral suspension was inoculated onto a confluent monolayer washed three times with 10 mL of Earls Balanced Salt Solution (EBSS) from Gibco BRL Cat #450-1 100EL to remove serum. The virus was allowed to spread by gentle rocking of the flask. The flask was kept at 37°C for 50 min. to allow for virus adsorption.
Supplemented MEM without serum was added and incubated for 24 hours. When 75%
Cytopathic effect (CPE) was observed. The virus was separated from the cells by three rapid freeze thawing followed by gentle centrifugation at 1500 rpm for 15 min. The supernatant which contained the virus was dispensed in aliquots of 200~L and stored at -80°C. The virus titre was determined using a plaque assay method.
Organic Load: Fetal bovine serum was used as the organic load in this study.
It was added to the test virus suspension at a final concentration of 5%.
The testing used flat stainless steel discs as the hard surface carriers. The organism used for the virucide tests was polio type 1 (Sabin) suspended in fetal bovine serum at a final concentration of 5%. Carrier inoculation involved placing 10~L (between 10g-lO6 plaque forming units) of the testvirus suspension on the center of each stainless steel carrier and allowing the inoculum to dry for 1 hour.
The dried itioculum was covered with the use dilution of the disinfectant for the specified contact tune and temperature. At the end of the contact time, EBSS was used as neutralizer/eluent for recovering the reaction mixture from the carrier. The eluent was serially diluted and plaque assay done to obtain titre of virus remaining.
Each formulation ~~las tested at a contact time of 10 minutes. Control carriers were used in the same manner as test carriers except sterile EBSS was applied to the dried inoculum instead of the chemical formulation. The number of test carriers was 5. Three control carriers were incorporated ul each. The results are reported as loglo reductions in viability in reference with the control carriers. For the formulation to be considered virucidal, it was expected to reduce the viable titre of all the test organisms by a minimum of 4 loglo under the conditions of this test.
Results The results of virucidal tests are showTn in Tables 3 and ~. Table ~ shows that formula #1 was not virucidal, it had little or no activity against poliovirus at a contact time of 10 minutes at 20=1°C.
Table 3. The germicidal activity of the formulation #1 against Polio type 1 (Sabin) ExperimentDate of Contact Average PFU/ControlPFU/Test AverageLogy Expt Time Carrier Garner I Reduction minutes ~
I
1 i Sep 9, 199710 1.X105 1.8X104 j 1 ~
I 2 ~ Sep 22, 10 7.2XI04 I.9XI04 j 1997 I I I I I I I
The polivirus was retested with formula #2 which increased the concentration of two of the ingredients. Formula #2 is a preferred embodiment of the inventive formula that consists of a solution of 10% sodium bromide w/v, 5% peracetic acid w/v, 3% phthalic acid dipotassium salt 98% w/v, 4% acetanilide w/v, 15% isopropanol v/v, 64% water v/v, and 1% soap v/v. The test methods used were identical of those describcd above. Formula #2 ~.~.ras able to reduce the ~~:~us titre by more than 4 loglo (Table 4).
Table 4. The germicidal activity of the inventive formulation against Poliovirus type 1 (Sabin) ExperimentDate of Contact Average PFU/ControlPFU/Test AverageLogo I I Expt Time Carrier I Carrier I Reduction I minutes I
I
1 I Nov 24, 10 I 6.8X104 0 4.83 I
I
2 ~ Nov 25, ~ 10 i 1.25X10 I 0 i 5.10 I
1~~
7,4 f'nn~l_ycin_n_ Formula #2 proved virucidal.
Claims (16)
1. A cleaning and disinfecting preparation with mycobactericidal, anti viral, germicidal and fungicidal action containing a solution of 10% sodium bromide w/v, 5%
peracetic acid w/v, 3% phthalic acid dipotassium salt 98% w/v, 4% acetanilide w/v, 15%
isopropanol v/v, and 64% water v/v.
peracetic acid w/v, 3% phthalic acid dipotassium salt 98% w/v, 4% acetanilide w/v, 15%
isopropanol v/v, and 64% water v/v.
2. A preparation according to claim 1 that also comprises a detergent.
3. A solution of 10% sodium bromide w/v, 5% peracetic acid w/v, 3% phthalic acid dipotassium salt 98% w/v, 4% acetamide w/v, 15% isopropanol v/v, 64% water v/v, and 1 % soap v/v for use as a cleaning and disinfecting preparation with mycobactericidal, anti viral, germicidal and fungicidal action.
4. A solution of 7-13% sodium bromide w/v, 3-6% peracetic acid w/v, 2-4%
phthalic acid dipotassium salt 98% w/v, 3-5% acetanilide w/v, 12-16% isopropanol v/v, 64%
water v/v, for use as a cleaning and disinfecting preparation with mycobactericidal, germicidal and fungicidal action.
phthalic acid dipotassium salt 98% w/v, 3-5% acetanilide w/v, 12-16% isopropanol v/v, 64%
water v/v, for use as a cleaning and disinfecting preparation with mycobactericidal, germicidal and fungicidal action.
5. A preparation according to airy of the preceding claims that is diluted 1:5 - 1:10 in water.
6. A preparation according to airy of the preceding claims wherein said preparation contains a suitable catalisator.
7. A preparation according to any of the preceding claims that is used as a pesticide.
8. A preparation according to any of the preceding claims that is used as a preservative for flowers.
9. A preparation according to any of the preceding claims that is used as an external disinfectant.
10. A preparation according to any of the preceding claims that is prepared as an ointment.
11. A preparation according to any of the preceding claims that is prepared for use in an aerosol container.
12. A preparation according to any of the preceding claims that is added to a solid soap preparation.
13. A preparation according to any of the preceding claims that is used on saturated tissues.
14. A preparation according to any of the preceding claims that is added to a detergent.
15. A preparation according to any of the preceding claims that is prepared as a dry powder.
16. A method for cleaning and disinfecting a contaminated object or area comprising application of a preparation according to any of the preceding claims to the said object or area.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL128374 | 1999-02-04 | ||
| IL12837499A IL128374A0 (en) | 1999-02-04 | 1999-02-04 | Wide range cleaning and disinfecting preparations |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2297350A1 true CA2297350A1 (en) | 2000-08-04 |
Family
ID=11072458
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2297350 Abandoned CA2297350A1 (en) | 1999-02-04 | 2000-02-02 | Wide range cleaning and disinfecting preparation |
Country Status (2)
| Country | Link |
|---|---|
| CA (1) | CA2297350A1 (en) |
| IL (1) | IL128374A0 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2001253777B2 (en) * | 2000-04-28 | 2005-05-19 | Ecolab Inc. | Antimicrobial composition |
-
1999
- 1999-02-04 IL IL12837499A patent/IL128374A0/en unknown
-
2000
- 2000-02-02 CA CA 2297350 patent/CA2297350A1/en not_active Abandoned
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2001253777B2 (en) * | 2000-04-28 | 2005-05-19 | Ecolab Inc. | Antimicrobial composition |
Also Published As
| Publication number | Publication date |
|---|---|
| IL128374A0 (en) | 2000-01-31 |
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