CA2293810C - Desaturases d'acide gras et leurs sequences mutantes - Google Patents

Desaturases d'acide gras et leurs sequences mutantes Download PDF

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Publication number
CA2293810C
CA2293810C CA002293810A CA2293810A CA2293810C CA 2293810 C CA2293810 C CA 2293810C CA 002293810 A CA002293810 A CA 002293810A CA 2293810 A CA2293810 A CA 2293810A CA 2293810 C CA2293810 C CA 2293810C
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CA
Canada
Prior art keywords
fatty acid
delta
plants
gene
mutation
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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CA002293810A
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English (en)
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CA2293810A1 (fr
Inventor
Lorin R. Debonte
Zhegong Fan
Basil S. Shorrosh
Guo-Hua Miao
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Cargill Inc
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Cargill Inc
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Priority to CA002575962A priority Critical patent/CA2575962A1/fr
Publication of CA2293810A1 publication Critical patent/CA2293810A1/fr
Application granted granted Critical
Publication of CA2293810C publication Critical patent/CA2293810C/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne des graines, des plantes et des huiles présentant une teneur élevée en acide oléique; une faible teneur en acide linoléique; une faible teneur en acide linoléique plus un acide linolénique; et des propriétés fonctionnelles ou alimentaires avantageuses. L'invention concerne des plantes présentant une mutation dans un gène à désaturases d'acide gras delta-12 ou delta-15. Les plantes sont, de préférence, du colza et du tournesol. Les plantes porteuses de tels gènes mutants présentent, dans leurs graines, une composition d'acide gras modifié. Selon un mode de réalisation, la plante présente une mutation dans une région comportant le motif conservé His-Xaa-Xaa-Xaa-His, rencontré dans les désaturases d'acide gras delta-12 et delta-15. Un motif préféré comporte la séquence His-Glu-Cys-Gly-His. Une mutation préférée de ce motif comporte la séquence d'acides aminés His-Lys-Cys-Gly-His. L'invention concerne, en outre, des fragments d'acide nucléique renfermant une séquence mutante de gènes à désaturases d'acide gras delta-12 ou delta-15.
CA002293810A 1997-06-12 1998-06-11 Desaturases d'acide gras et leurs sequences mutantes Expired - Lifetime CA2293810C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002575962A CA2575962A1 (fr) 1997-06-12 1998-06-11 Desaturases d'acides gras et sequences mutantes de ces enzymes

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US87410997A 1997-06-12 1997-06-12
US08/874,109 1997-06-12
PCT/US1998/012332 WO1998056239A1 (fr) 1997-06-12 1998-06-11 Desaturases d'acide gras et leurs sequences mutantes

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002575962A Division CA2575962A1 (fr) 1997-06-12 1998-06-11 Desaturases d'acides gras et sequences mutantes de ces enzymes

Publications (2)

Publication Number Publication Date
CA2293810A1 CA2293810A1 (fr) 1998-12-17
CA2293810C true CA2293810C (fr) 2010-03-09

Family

ID=25362994

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002293810A Expired - Lifetime CA2293810C (fr) 1997-06-12 1998-06-11 Desaturases d'acide gras et leurs sequences mutantes

Country Status (5)

Country Link
EP (1) EP0987936A4 (fr)
JP (1) JP4209949B2 (fr)
AU (1) AU750363B2 (fr)
CA (1) CA2293810C (fr)
WO (1) WO1998056239A1 (fr)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6483008B1 (en) * 1990-08-15 2002-11-19 Calgene Llc Methods for producing plants with elevated oleic acid content
AU771958B2 (en) * 1998-08-04 2004-04-08 Cargill Incorporated Plant fatty acid desaturase promoters
US6713117B1 (en) 1998-10-02 2004-03-30 Dharma Kodali Vegetable oil having elevated stearic acid content
CA2284246A1 (fr) 1999-10-01 2001-04-01 Agriculture And Agrifood Canada Of Agriculture And Agri-Food Desaturases d'acides gras vegetaux et leurs alleles
US20020045232A1 (en) * 2000-05-09 2002-04-18 Xiao Qiu Production of conjugated linoleic and linolenic acids in plants
US7566813B2 (en) 2002-03-21 2009-07-28 Monsanto Technology, L.L.C. Nucleic acid constructs and methods for producing altered seed oil compositions
US8304610B2 (en) 2002-05-15 2012-11-06 Viterra, Inc. Brassica juncea lines with high oleic acid profile in seed oil
AU2004251638A1 (en) * 2003-05-30 2005-01-06 Cargill, Incorporated Methods of making plants that exhibit enhanced disease resistance
US7807849B2 (en) 2004-04-22 2010-10-05 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
BR122019013214B1 (pt) 2004-04-22 2022-04-12 Commonwealth Scientific And Industrial Research Organisation Polinucleotídeo isolado, vetor, célula recombinante, método para produzir uma célula recombinante, método para produzir um ou mais ácido graxo(s) poliinsaturado de cadeia longa, método para produzir uma semente transgênica, método para produzir óleo, método para produzir uma semente oleaginosa, extrato da semente transgênica, composição compreendendo o referido extrato, ração e uso da referida célula recombinante ou extrato
AU2004203364B2 (en) * 2004-07-15 2011-04-07 Nutrien Ag Solutions (Canada) Inc. High oleic acid brassica juncea
EP1806398A1 (fr) * 2006-01-04 2007-07-11 Monsanto S.A.S. Mutants de FAD-2 et plantes à teneur élevée en acide oléique
CN101421406B (zh) 2006-02-13 2016-08-31 孟山都技术有限公司 用于产生改变的种子油组成的核酸构建体和方法
EP1837397A1 (fr) 2006-03-21 2007-09-26 Monsanto S.A.S. Mutants de FAD-2 et plantes à teneur élevée en acide oléique
WO2008025068A1 (fr) 2006-08-29 2008-03-06 Commonwealth Scientific And Industrial Research Organisation synthèse d'acides gras
UY32145A (es) 2008-09-29 2010-04-30 Monsanto Technology Llc Evento transgénico de soja mon87705 y métodos para detectar el mismo
CA2743880C (fr) 2008-11-18 2020-08-18 James Robertson Petrie Cellules recombinantes possedant une activite elongase a5 exogene et methodes de production d'acide docosapentaenoique
CN105557503A (zh) * 2009-07-08 2016-05-11 密苏里大学管委会 利用传统大豆育种技术培育高油酸大豆的方法
JP5882225B2 (ja) 2009-12-18 2016-03-09 カーギル・インコーポレイテッド 低総飽和脂肪酸含有率を有するオイルを産するアブラナ属植物
WO2011150028A2 (fr) 2010-05-25 2011-12-01 Cargill, Incorporated Plantes du genre brassica donnant des huiles à faible teneur en acide alpha-linolénique
US9695434B2 (en) 2010-05-25 2017-07-04 Cargill, Incorporated Brassica plants yielding oils with a low alpha linolenic acid content
GB201103569D0 (en) * 2011-03-02 2011-04-13 Plant Bioscience Ltd Mutants
MY187101A (en) 2012-06-15 2021-08-31 Commw Scient Ind Res Org Production of long chain polyunsaturated fatty acids in plant cells
UA118090C2 (uk) 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
UA122768C2 (uk) 2013-12-18 2021-01-06 Коммонвелз Сайнтіфік Енд Індастріал Рисерч Организейшн Екстрагований рослинний ліпід, що містить довголанцюгові поліненасичені жирні кислоти
AU2015281791A1 (en) 2014-06-27 2017-01-12 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
US10494642B2 (en) 2015-04-28 2019-12-03 Basf Agricultural Solutions Seed, Us Llc Brassica plants with modified seed oil composition
CA3047768A1 (fr) 2019-06-21 2020-12-21 BASF Agricultural Solutions Seed US LLC Variete de canola hybride 7cn0425
US11997965B2 (en) 2020-04-24 2024-06-04 BASF Agricultural Solutions Seed US LLC Canola hybrid variety 8CN0001
US11672218B2 (en) 2020-04-24 2023-06-13 BASF Agricultural Solutions Seed US LLC Canola hybrid variety 7CN0065
US11997966B2 (en) 2020-04-24 2024-06-04 BASF Agricultural Solutions Seed US LLC Canola hybrid variety 7CN0020

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0616644T3 (da) * 1991-12-04 2003-10-27 Du Pont Fedtsyredesaturase-gener fra planter
JP3818540B2 (ja) * 1992-11-17 2006-09-06 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 植物由来のミクロソームデルタ−１２脂肪酸デサチュラーゼおよび関連酵素の遺伝子
GB9324823D0 (en) * 1993-12-02 1994-01-19 Davy Mckee London Process
DE69635905T2 (de) * 1995-12-14 2006-12-21 Cargill, Inc., Wayzata Pflanzen mit mutierten Sequenzen, welche ein veränderten Fettsäuregehalt vermitteln
CA2180386C (fr) * 1996-07-03 2006-09-12 Lorin R. Debonte Huile de colza possedant une teneur plus elevee en acide oleique et en acide linolenique

Also Published As

Publication number Publication date
AU8071598A (en) 1998-12-30
JP4209949B2 (ja) 2009-01-14
EP0987936A4 (fr) 2001-03-14
AU750363B2 (en) 2002-07-18
CA2293810A1 (fr) 1998-12-17
WO1998056239A1 (fr) 1998-12-17
JP2002508659A (ja) 2002-03-19
EP0987936A1 (fr) 2000-03-29

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Effective date: 20180611