CA2287427A1 - Test par fluorescence homogene pour mesurer l'effet des composes sur l'expression d'un gene - Google Patents

Test par fluorescence homogene pour mesurer l'effet des composes sur l'expression d'un gene Download PDF

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Publication number
CA2287427A1
CA2287427A1 CA002287427A CA2287427A CA2287427A1 CA 2287427 A1 CA2287427 A1 CA 2287427A1 CA 002287427 A CA002287427 A CA 002287427A CA 2287427 A CA2287427 A CA 2287427A CA 2287427 A1 CA2287427 A1 CA 2287427A1
Authority
CA
Canada
Prior art keywords
optical signal
extracellular protein
cells
operably linked
promoter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002287427A
Other languages
English (en)
Inventor
Damien J. Dunnington
Keith J. Moore
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline Beecham Ltd
SmithKline Beecham Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2287427A1 publication Critical patent/CA2287427A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un système de test par fluorescence de gène reporter qui ne nécessite aucune étape de lavage ou de lyse cellulaire, ce système pouvant effectuer une détection par fluorescence à résolution temporelle et étant approprié au multiplexage. L'invention concerne également un procédé permettant d'effectuer une détection à haut rendement à l'aide d'un système de test de gène reporter par fluorescence. L'invention concerne enfin un procédé de détection de bibliothèques combinatoires utilisé dans le système précité.
CA002287427A 1997-04-22 1998-04-22 Test par fluorescence homogene pour mesurer l'effet des composes sur l'expression d'un gene Abandoned CA2287427A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US4452797P 1997-04-22 1997-04-22
US60/044,527 1997-04-22
PCT/US1998/008101 WO1998048274A1 (fr) 1997-04-22 1998-04-22 Test par fluorescence homogene pour mesurer l'effet des composes sur l'expression d'un gene

Publications (1)

Publication Number Publication Date
CA2287427A1 true CA2287427A1 (fr) 1998-10-29

Family

ID=21932889

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002287427A Abandoned CA2287427A1 (fr) 1997-04-22 1998-04-22 Test par fluorescence homogene pour mesurer l'effet des composes sur l'expression d'un gene

Country Status (4)

Country Link
EP (1) EP0977988A4 (fr)
JP (1) JP2002511744A (fr)
CA (1) CA2287427A1 (fr)
WO (1) WO1998048274A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001062922A2 (fr) * 2000-02-24 2001-08-30 Incyte Genomics, Inc. Molecules destinees a la detection et au traitement de maladies
CA2418496A1 (fr) * 2000-02-24 2001-08-30 Incyte Genomics, Inc. Molecules secretoires
WO2001062927A2 (fr) * 2000-02-24 2001-08-30 Incyte Genomics Inc Molecules utilisees a des fins diagnostiques et therapeutiques
CA2421265A1 (fr) * 2000-09-05 2002-03-14 Incyte Genomics, Inc. Molecules utilisees a des fins diagnostiques et therapeutiques
US20080249103A1 (en) * 2006-11-15 2008-10-09 Sirtris Pharmaceuticals, Inc. Sirtuin polymorphisms and methods of use thereof

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0327960A1 (fr) * 1988-02-11 1989-08-16 F. Hoffmann-La Roche Ag Formes sécrétables de phosphatase alcaline
US5156951A (en) * 1989-07-13 1992-10-20 Becton Dickinson And Company Detecting immunological changes in HIV infected patient samples
AU7667191A (en) * 1990-04-19 1991-11-11 General Hospital Corporation, The Screening assays for compounds which inhibit the binding of c-myc to dna
US5744300A (en) * 1993-03-24 1998-04-28 Geron Corporation Methods and reagents for the identification and regulation of senescence-related genes
AU7844894A (en) * 1993-09-27 1995-04-18 University Of Massachusetts Medical Center Receptor-activated reporter systems
GB9320562D0 (en) * 1993-10-06 1993-11-24 Zeneca Ltd Novel assay and applications
DE69428319T2 (de) * 1993-10-13 2002-07-04 Univ Northeastern Ohio Genomische DNS für menschliche Cholesterol-7-alpha Hydroxylase und Verfahren zu dessen Verwendung
US5506102A (en) * 1993-10-28 1996-04-09 Ligand Pharmaceuticals Incorporated Methods of using the A form of the progesterone receptor to screen for antagonists of steroid intracellar receptor-mediated transcription
US6008373A (en) * 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
US5747338A (en) * 1996-08-15 1998-05-05 Chiron Corporation Method and construct for screening for inhibitors of transcriptional activation

Also Published As

Publication number Publication date
JP2002511744A (ja) 2002-04-16
EP0977988A4 (fr) 2004-06-30
WO1998048274A1 (fr) 1998-10-29
EP0977988A1 (fr) 2000-02-09

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