CA2283251A1 - Device and method for carrying out fluorescence immunoassays - Google Patents
Device and method for carrying out fluorescence immunoassays Download PDFInfo
- Publication number
- CA2283251A1 CA2283251A1 CA002283251A CA2283251A CA2283251A1 CA 2283251 A1 CA2283251 A1 CA 2283251A1 CA 002283251 A CA002283251 A CA 002283251A CA 2283251 A CA2283251 A CA 2283251A CA 2283251 A1 CA2283251 A1 CA 2283251A1
- Authority
- CA
- Canada
- Prior art keywords
- light
- base plate
- boundary surface
- light source
- disposed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
Abstract
The invention relates to a device and a method for carrying out quantitative fluorescence immunoassays by means of evanescent field excitation. Various known biochemical assays of general receptor-ligand systems can be used. For this purpose, light of at least one light source is directed at an angle .alpha. onto the boundary surface of two media which have different refractive indices. A light source is selected which emits substantially monochromatic light with a wavelength which is suitable for exciting a marking substance. The light is directed onto the boundary surface which is disposed between an optically transparent base plate made of a material where the refractive index n1 is greater than the refractive index n2 of the material above the boundary surface, and a cuvette-shaped receiving region for the sample. The receiving region is covered with a covering plate on the side disposed opposite the base plate.
Arranged between base plate and covering plate is at least one functional layer and a detector for detecting the fluorescent light being disposed on the same side of the base plate as the light source.
Arranged between base plate and covering plate is at least one functional layer and a detector for detecting the fluorescent light being disposed on the same side of the base plate as the light source.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19711281A DE19711281C1 (en) | 1997-03-18 | 1997-03-18 | Device for fluorescent specific-binding tests using evanescent field excitation |
DE19711281.1 | 1997-03-18 | ||
PCT/DE1998/000729 WO1998041843A2 (en) | 1997-03-18 | 1998-03-11 | Device and method for carrying out fluorescence immunotests |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2283251A1 true CA2283251A1 (en) | 1998-09-24 |
CA2283251C CA2283251C (en) | 2007-11-27 |
Family
ID=7823792
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002283251A Expired - Fee Related CA2283251C (en) | 1997-03-18 | 1998-03-11 | Device and method for carrying out fluorescence immunoassays |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0968412B1 (en) |
JP (1) | JP2001515592A (en) |
AT (1) | ATE227425T1 (en) |
CA (1) | CA2283251C (en) |
DE (2) | DE19711281C1 (en) |
DK (1) | DK0968412T3 (en) |
RU (1) | RU2194972C2 (en) |
WO (1) | WO1998041843A2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11346839B2 (en) | 2018-08-03 | 2022-05-31 | Insingulo Ab | Method for determining the interaction between a ligand and a receptor |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19933458B4 (en) * | 1999-07-15 | 2015-08-20 | Eppendorf Ag | Equipment and systems for handling liquid samples |
DE10001116C2 (en) * | 2000-01-13 | 2002-11-28 | Meinhard Knoll | Device and method for the optical or electrochemical quantitative determination of chemical or biochemical substances in liquid samples |
DE10058095C2 (en) * | 2000-11-03 | 2003-12-18 | Fraunhofer Ges Forschung | Device for the determination of analytes by chemiluminescence |
DE10105711B4 (en) * | 2001-02-08 | 2005-03-10 | Ibidi Gmbh | Sample carriers for chemical and biological samples |
DE10307802A1 (en) * | 2003-02-24 | 2004-09-09 | Advalytix Ag | Apparatus for the analysis of specific binding reactions, between protein or DNA probe macro molecules and a fluid containing sample macro molecules, has a spot carrier which is transparent and non-luminescent |
DE102004022263A1 (en) * | 2004-05-06 | 2005-12-15 | Clondiag Chip Technologies Gmbh | Apparatus and method for detecting molecular interactions |
JP2008032420A (en) * | 2006-07-26 | 2008-02-14 | National Institute Of Advanced Industrial & Technology | Method and member for weakening background fluorescence in evernescent wave exciting fluorescence observation |
DE102007019695B4 (en) * | 2007-04-24 | 2009-08-13 | Analytik Jena Ag | Cuvette for the optical analysis of small volumes |
DE102010038431A1 (en) | 2010-07-26 | 2012-01-26 | Diasys Diagnostic Systems Gmbh | Measuring cassette and measuring device for the detection of target molecules in a liquid sample by measuring fluorescence emission after excitation in the evanescent field |
DE102022207704A1 (en) | 2022-07-27 | 2024-02-01 | Robert Bosch Gesellschaft mit beschränkter Haftung | Distributor unit for an analysis device for analyzing a sample contained in a microfluidic cartridge, analysis device and method for producing a body unit for a distribution unit |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3814370A1 (en) * | 1988-04-28 | 1989-11-09 | Boehringer Mannheim Gmbh | TEST PROVIDER FOR THE ANALYSIS OF A SAMPLING FLUID, METHOD FOR CARRYING OUT SUCH ANALYSIS AND METHOD OF PRODUCTION |
SE462408B (en) * | 1988-11-10 | 1990-06-18 | Pharmacia Ab | OPTICAL BIOSENSOR SYSTEM USING SURFACE MONITORING RESONSE FOR THE DETECTION OF A SPECIFIC BIOMOLIC CYCLE, TO CALIBRATE THE SENSOR DEVICE AND TO CORRECT FOUND BASELINE OPERATION IN THE SYSTEM |
GB8827853D0 (en) * | 1988-11-29 | 1988-12-29 | Ares Serono Res & Dev Ltd | Sensor for optical assay |
DE4121493A1 (en) * | 1991-06-28 | 1993-01-07 | Draegerwerk Ag | ANALYSIS DEVICE FOR THE QUANTITATIVE, IMMUNOLOGICAL DETERMINATION OF POLLUTANTS |
ATE209782T1 (en) * | 1993-05-18 | 2001-12-15 | Univ Utah Res Found | DEVICE AND METHOD FOR HOMOGENEOUS MULTIANALYTE IMMUNO ASSAY |
DE19611025A1 (en) * | 1996-03-20 | 1997-09-25 | Inst Chemo Biosensorik | Optical fiber optic sensor based on the resonant optical excitation of surface plasma waves |
DE19628002C1 (en) * | 1996-07-11 | 1997-12-18 | Inst Chemo Biosensorik | Device and method for carrying out fluorescence immunoassays |
-
1997
- 1997-03-18 DE DE19711281A patent/DE19711281C1/en not_active Expired - Fee Related
-
1998
- 1998-03-11 AT AT98923995T patent/ATE227425T1/en active
- 1998-03-11 EP EP98923995A patent/EP0968412B1/en not_active Expired - Lifetime
- 1998-03-11 CA CA002283251A patent/CA2283251C/en not_active Expired - Fee Related
- 1998-03-11 WO PCT/DE1998/000729 patent/WO1998041843A2/en active IP Right Grant
- 1998-03-11 DK DK98923995T patent/DK0968412T3/en active
- 1998-03-11 JP JP54002398A patent/JP2001515592A/en active Pending
- 1998-03-11 RU RU99121646/28A patent/RU2194972C2/en not_active IP Right Cessation
- 1998-03-11 DE DE59806188T patent/DE59806188D1/en not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11346839B2 (en) | 2018-08-03 | 2022-05-31 | Insingulo Ab | Method for determining the interaction between a ligand and a receptor |
Also Published As
Publication number | Publication date |
---|---|
RU2194972C2 (en) | 2002-12-20 |
JP2001515592A (en) | 2001-09-18 |
WO1998041843A3 (en) | 1998-11-19 |
WO1998041843A2 (en) | 1998-09-24 |
DE59806188D1 (en) | 2002-12-12 |
EP0968412B1 (en) | 2002-11-06 |
CA2283251C (en) | 2007-11-27 |
DE19711281C1 (en) | 1998-04-16 |
ATE227425T1 (en) | 2002-11-15 |
DK0968412T3 (en) | 2003-03-10 |
EP0968412A2 (en) | 2000-01-05 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |
Effective date: 20160311 |
|
MKLA | Lapsed |
Effective date: 20160311 |