CA2260095A1 - Process and device for carrying out quantitative, fluorescence affinity tests - Google Patents

Process and device for carrying out quantitative, fluorescence affinity tests

Info

Publication number
CA2260095A1
CA2260095A1 CA002260095A CA2260095A CA2260095A1 CA 2260095 A1 CA2260095 A1 CA 2260095A1 CA 002260095 A CA002260095 A CA 002260095A CA 2260095 A CA2260095 A CA 2260095A CA 2260095 A1 CA2260095 A1 CA 2260095A1
Authority
CA
Canada
Prior art keywords
base plate
light
antibody
carrying
receiving area
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002260095A
Other languages
French (fr)
Other versions
CA2260095C (en
Inventor
Andreas Katerkamp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PES GESELLSCHAFT fur MEDIZINISCHE DIAGNOSE-SYSTEME MBH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19628002A external-priority patent/DE19628002C1/en
Application filed by Individual filed Critical Individual
Publication of CA2260095A1 publication Critical patent/CA2260095A1/en
Application granted granted Critical
Publication of CA2260095C publication Critical patent/CA2260095C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Optics & Photonics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

A process and device are disclosed for carrying out in particular quantitative fluorescence immunity tests by means of evancacent field excitation, possibly on the basis of various known biochemical assays of systems generally composed of receptor-ligands. However, antibody-antigen systems are preferably evaluated. The invention should enable quantitative fluorescence immunity tests with various known biochemical assays to be carried out with a very simple device. For that purpose, a light source (7, 7) is used which emits rays of an almost monochromatic light having a wavelength which causes a marking substance bound to the antibody to become fluorescent. The light rays are directed at an angle .alpha. determined by a depth of penetration d previously determined for the evanescent field onto a boundary surface (20) between an optically transparent base plate (i) made of a material with a refraction index n1 higher than the refraction index n2 of the material above the boundary surface (20) and a cuvette-shaped sample-receiving area (2). The sample receiving area (2) is covered at the side opposite to the base plate (1) by a covering plate (3) and a detector (5) for sensing fluorescent light is arranged at the same side of the base plate (1) as the light source (7).
CA 2260095 1996-07-11 1997-07-11 Process and device for carrying out quantitative, fluorescence affinity tests Expired - Fee Related CA2260095C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19628002A DE19628002C1 (en) 1996-07-11 1996-07-11 Device and method for carrying out fluorescence immunoassays
DE19628002.8 1996-07-11
PCT/DE1997/001499 WO1998002732A1 (en) 1996-07-11 1997-07-11 Process and device for carrying out quantitative, fluorescence affinity tests

Publications (2)

Publication Number Publication Date
CA2260095A1 true CA2260095A1 (en) 1998-01-22
CA2260095C CA2260095C (en) 2006-09-26

Family

ID=37057092

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2260095 Expired - Fee Related CA2260095C (en) 1996-07-11 1997-07-11 Process and device for carrying out quantitative, fluorescence affinity tests

Country Status (1)

Country Link
CA (1) CA2260095C (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108828206A (en) * 2018-05-24 2018-11-16 广州天宝颂原生物科技开发有限公司 A kind of dry type fluorescence immunity analyzer and its test method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108828206A (en) * 2018-05-24 2018-11-16 广州天宝颂原生物科技开发有限公司 A kind of dry type fluorescence immunity analyzer and its test method

Also Published As

Publication number Publication date
CA2260095C (en) 2006-09-26

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Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20160711

MKLA Lapsed

Effective date: 20160711