CA2275885A1 - Transgenic potatoes having reduced levels of alpha glucan l- or h-type tuber phosphorylase activity with reduced cold-sweetening - Google Patents

Transgenic potatoes having reduced levels of alpha glucan l- or h-type tuber phosphorylase activity with reduced cold-sweetening Download PDF

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Publication number
CA2275885A1
CA2275885A1 CA002275885A CA2275885A CA2275885A1 CA 2275885 A1 CA2275885 A1 CA 2275885A1 CA 002275885 A CA002275885 A CA 002275885A CA 2275885 A CA2275885 A CA 2275885A CA 2275885 A1 CA2275885 A1 CA 2275885A1
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CA
Canada
Prior art keywords
reduced
gltp
ghtp
potato
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002275885A
Other languages
French (fr)
Other versions
CA2275885C (en
Inventor
Lawrence Michael Kawchuk
John David Armstrong
Dermot Roborg Lynch
Norman Richard Knowles
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agriculture and Agri Food Canada AAFC
Original Assignee
Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada
Lawrence Michael Kawchuk
John David Armstrong
Dermot Roborg Lynch
Norman Richard Knowles
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/868,786 external-priority patent/US5998701A/en
Application filed by Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada, Lawrence Michael Kawchuk, John David Armstrong, Dermot Roborg Lynch, Norman Richard Knowles filed Critical Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada
Publication of CA2275885A1 publication Critical patent/CA2275885A1/en
Application granted granted Critical
Publication of CA2275885C publication Critical patent/CA2275885C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

Abstract

Potato plants which exhibit reduced levels of a glucan L-type tuber phosphorylase (GLTP) or a glucan H-type tuber phosphorylase (GHTP) enzyme activity within the potato tuber are provided. The conversion of starches to sugars in potato tubers, particularly when stored at temperatures below 7 ° C, is reduced in tubers exhibiting reduced GLTP or GHTP enzyme activity. Reducing cold-sweetening in potatoes allows for potato storage at cooler temperatures, resulting in prolonged dormancy, reduced incidence of disease, and increased storage life. Methods for producing potato plants which produce tubers exhibiting reduced GLTP or GHTP
enzyme activity are also provided. Reduction of GLTP or GHTP activity within the potato tuber may be accomplished by such techniques as suppression of gene expression using homologous antisense RNA, the use of co-suppression, regulatory silencing sequences, chemical and protein inhibitors, or the use of site-directed mutagenesis or the isolation of alternative alleles to obtain GLTP or GHTP variants with reduced starch affinity or activity.
CA002275885A 1997-02-10 1998-02-05 Transgenic potatoes having reduced levels of alpha glucan l- or h-type tuber phosphorylase activity with reduced cold-sweetening Expired - Fee Related CA2275885C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US3694697P 1997-02-10 1997-02-10
US60/036,946 1997-02-10
US08/868,786 US5998701A (en) 1997-06-04 1997-06-04 Potatoes having improved quality characteristics and methods for their production
US08/868,786 1997-06-04
PCT/CA1998/000055 WO1998035051A1 (en) 1997-02-10 1998-02-05 Transgenic potatoes having reduced levels of alpha glucan l- or h-type tuber phosphorylase activity with reduced cold-sweetening

Publications (2)

Publication Number Publication Date
CA2275885A1 true CA2275885A1 (en) 1998-08-13
CA2275885C CA2275885C (en) 2007-01-09

Family

ID=26713651

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002275885A Expired - Fee Related CA2275885C (en) 1997-02-10 1998-02-05 Transgenic potatoes having reduced levels of alpha glucan l- or h-type tuber phosphorylase activity with reduced cold-sweetening

Country Status (11)

Country Link
EP (1) EP1009839A1 (en)
JP (1) JP2001511007A (en)
CN (2) CN1246894A (en)
AR (1) AR011121A1 (en)
AU (1) AU724942B2 (en)
BR (1) BR9807214A (en)
CA (1) CA2275885C (en)
HU (1) HUP0000542A3 (en)
NZ (1) NZ336766A (en)
PL (1) PL334962A1 (en)
WO (1) WO1998035051A1 (en)

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AUPO069996A0 (en) 1996-06-27 1996-07-18 Australian National University, The Manipulation of plant cellulose
US7534934B2 (en) 2002-02-20 2009-05-19 J.R. Simplot Company Precise breeding
CN102978236B (en) 2002-02-20 2015-09-09 J·R·西姆普罗特公司 Precise breeding
US8250818B2 (en) * 2004-03-03 2012-08-28 Robert Tremblay Self-centering energy dissipative brace apparatus with tensioning elements
FR2868080B1 (en) * 2004-03-29 2007-11-16 Genoplante Valor Soc Par Actio METHOD FOR IMPROVING PLANTS
JP4654089B2 (en) * 2004-12-03 2011-03-16 新日本製鐵株式会社 Chromate-free resin composite vibration damping material with excellent durability adhesion
CN100398758C (en) * 2004-12-13 2008-07-02 东南大学 High performance composite shock absorber
CN100516394C (en) * 2005-12-02 2009-07-22 卢锐 Laminated steel plate energy-dissipation shock-absorbing damp
DE102007030883A1 (en) 2007-07-03 2009-01-08 Schaeffler Kg Seal for a hydrostatic linear guide
US8546537B2 (en) * 2009-02-20 2013-10-01 The University Of Tokyo Streptavidin having low immunogenicity and use thereof
CN102348532A (en) * 2009-03-27 2012-02-08 村田机械株式会社 Two-axis machine tool
EP2493286A2 (en) * 2009-10-26 2012-09-05 Agventure B.V. Hybrid seed potato breeding
FR2957998B1 (en) * 2010-03-29 2013-04-26 Eurocopter France DAMPER OF A BLADE, AND ROTOR PROVIDED WITH SUCH SHOCK ABSORBER
CN102409774B (en) * 2011-09-02 2013-07-31 北京交通大学 Viscoelastic damper used for buckling restrained braced building
CN103216017A (en) * 2012-01-18 2013-07-24 南京丹普科技工程有限公司 Viscoelastic damper
CN103276828B (en) * 2013-05-22 2015-08-19 太原理工大学 The curve rod-pulling type resistance to plucking device that short circuit is put more energy into
KR101516421B1 (en) * 2013-08-22 2015-05-26 김시언 Instant measuring method for glucose content of potato
CN104314192A (en) * 2014-10-22 2015-01-28 上海大学 Multilayer damper with displacement amplifying device
CN104453000A (en) * 2014-10-24 2015-03-25 大连理工大学 Composite type buckling-restrained brace filled with elastic materials
CN104534014B (en) * 2014-11-11 2016-10-12 上海宇航系统工程研究所 A kind of shearing-type damping pipe with damping property and vibration damping adapter
CN104895253B (en) * 2015-04-20 2017-04-26 北京工业大学 Damping steel bar connector
CN104879414B (en) * 2015-04-30 2017-04-05 北京空间机电研究所 A kind of Visco-elastic damping truss vibration isolator
CN105507494B (en) * 2016-01-15 2018-03-27 兰州理工大学 Overlap the replaceable coupling beam of tooth form steel plate composite damping energy-dissipating type and its construction method
CN106436951B (en) * 2016-10-18 2018-08-31 中国建筑第八工程局有限公司 Biting connecions two benches friction energy-dissipating damper
CN106481135B (en) * 2016-11-15 2019-04-12 东南大学 A kind of end has the buckling induction support of depression in centre type induction unit
CN106760834B (en) * 2016-11-15 2019-01-08 东南大学 A kind of end has the buckling induction support of the double-deck inner concave shape induction unit of elongated degree
CN107130831B (en) * 2017-05-25 2019-03-29 东南大学 A kind of multidimensional corrugated thickness viscoelastic layer is every vibration damping/shake device
CN115045947A (en) * 2022-06-09 2022-09-13 北京金茂人居环境科技有限公司 Axial magneto-rheological damping broadband vibration isolator
LU502613B1 (en) * 2022-08-01 2024-02-01 Plant Bioscience Ltd Methods of altering the starch granule profile in plants

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK198089D0 (en) * 1989-04-24 1989-04-24 Danske Spritfabrikker DNA MATERIALS AND USE THEREOF
DE4220758A1 (en) * 1992-06-24 1994-01-05 Inst Genbiologische Forschung DNA sequence and plasmids for the production of plants with a modified sucrose concentration
JPH08510645A (en) * 1993-05-28 1996-11-12 モンサント・カンパニー How to improve the quality of stored potatoes
KR100452853B1 (en) * 1995-12-27 2005-01-27 니뽄 다바코 산교 가부시키가이샤 Low temperature inducible promoter sequence

Also Published As

Publication number Publication date
CA2275885C (en) 2007-01-09
NZ336766A (en) 2000-08-25
EP1009839A1 (en) 2000-06-21
AR011121A1 (en) 2000-08-02
AU5849398A (en) 1998-08-26
BR9807214A (en) 2000-04-25
HUP0000542A2 (en) 2000-06-28
CN1300338A (en) 2001-06-20
PL334962A1 (en) 2000-03-27
CN1113143C (en) 2003-07-02
HUP0000542A3 (en) 2002-02-28
JP2001511007A (en) 2001-08-07
AU724942B2 (en) 2000-10-05
WO1998035051A1 (en) 1998-08-13
CN1246894A (en) 2000-03-08

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