CA2272890A1 - Non-aggregated fluorescent conjugates and method for obtaining them - Google Patents

Non-aggregated fluorescent conjugates and method for obtaining them Download PDF

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Publication number
CA2272890A1
CA2272890A1 CA002272890A CA2272890A CA2272890A1 CA 2272890 A1 CA2272890 A1 CA 2272890A1 CA 002272890 A CA002272890 A CA 002272890A CA 2272890 A CA2272890 A CA 2272890A CA 2272890 A1 CA2272890 A1 CA 2272890A1
Authority
CA
Canada
Prior art keywords
obtaining
fluorescent conjugates
carboxy
hydroxy
amino
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002272890A
Other languages
French (fr)
Other versions
CA2272890C (en
Inventor
Daniel Aspe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CIS Bio International SA
Original Assignee
Cis Bio International
Daniel Aspe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cis Bio International, Daniel Aspe filed Critical Cis Bio International
Publication of CA2272890A1 publication Critical patent/CA2272890A1/en
Application granted granted Critical
Publication of CA2272890C publication Critical patent/CA2272890C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Abstract

The invention concerns a method for obtaining a fluorescent conjugate between a carrier molecule having at least an amino, hydroxy, carboxy and/or sulphydryl group and a fluorophore reagent having at least a functional group capable of reacting with said amino, hydroxy, carboxy and/or sulphydryl group(s), which consists in bringing said molecule and said fluorophore reagent in the presence of an aqueous solution of a water-soluble macrocycle. The invention also concerns the conjugates obtained by this method and their use.
CA002272890A 1996-12-12 1997-12-12 Non-aggregated fluorescent conjugates and method for obtaining them Expired - Fee Related CA2272890C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9615261A FR2757162B1 (en) 1996-12-12 1996-12-12 NON-AGGREGATED FLUORESCENT CONJUGATES
FR96/15261 1996-12-12
PCT/FR1997/002288 WO1998026287A1 (en) 1996-12-12 1997-12-12 Non-aggregated fluorescent conjugates and method for obtaining them

Publications (2)

Publication Number Publication Date
CA2272890A1 true CA2272890A1 (en) 1998-06-18
CA2272890C CA2272890C (en) 2004-11-30

Family

ID=9498586

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002272890A Expired - Fee Related CA2272890C (en) 1996-12-12 1997-12-12 Non-aggregated fluorescent conjugates and method for obtaining them

Country Status (9)

Country Link
EP (1) EP0946870B1 (en)
JP (1) JP3955638B2 (en)
AT (1) ATE228656T1 (en)
AU (1) AU5489498A (en)
CA (1) CA2272890C (en)
DE (1) DE69717490T2 (en)
ES (1) ES2187834T3 (en)
FR (1) FR2757162B1 (en)
WO (1) WO1998026287A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19731741A1 (en) * 1997-07-23 1999-01-28 Deutsches Krebsforsch Conjugate to differentiate between diseased and healthy tissue
EP0934986A1 (en) * 1997-12-17 1999-08-11 Roche Diagnostics GmbH Dye-polysaccharide or -cyclosaccharide conjugates and their use as diagnostic agent
US6242430B1 (en) 1998-04-30 2001-06-05 Laboratory Of Molecular Biophotonics Cyclodextrin-based rotaxane dyes, labeling agent using the dye, and a method for labeling
DE10026623A1 (en) * 2000-05-29 2001-12-06 Heinz Langhals Perylene dyes with persistent fluorescence due to steric aggregation inhibition
JP4235550B2 (en) 2001-09-28 2009-03-11 キヤノン株式会社 Organic light emitting device
FR2840611B1 (en) * 2002-06-06 2005-09-09 Cis Bio Int FLUORESCENT ENTITY COMPRISING A FLUOROPHORE COVALENTLY ASSOCIATED WITH AT LEAST ONE OLIGONUCLEOTIDE AND COMPRISING AT LEAST ONE FUNCTIONAL GROUP AND USES THEREOF
FR2878853B1 (en) * 2004-12-07 2007-03-16 Biocydex Soc Par Actions Simpl METHOD FOR OBTAINING A FLUORESCENT COMPLEX, COMPLEX OBTAINED AND USE
JP2009506188A (en) * 2005-08-30 2009-02-12 エイジェンシー フォー サイエンス， テクノロジー アンド リサーチ Water-soluble fluorescent particles containing entangled fluorescent polymers and amphiphilic molecules
FR3045053B1 (en) * 2015-12-09 2018-01-05 Cisbio Bioassays TRIAZAPYRIDINOPHANE WATER-SOLUBLE COMPLEXING AGENTS AND CORRESPONDING FLUORESCENT LANTHANIDE COMPLEXES

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5068227A (en) * 1989-01-18 1991-11-26 Cyclex, Inc. Cyclodextrins as carriers
WO1991002040A1 (en) * 1989-08-04 1991-02-21 Kosak Kenneth M Cyclodextrin labels for nucleic acid and biochemical analysis
WO1991005605A1 (en) * 1989-10-10 1991-05-02 Kosak Kenneth M Cyclodextrin labels for immunoassay and biochemical analysis
ES2259791T3 (en) * 1993-07-13 2006-10-16 Abbott Laboratories VERY FLUORESCENT CONJUGATES OF POLYMERS AND INTERMEDIATES.

Also Published As

Publication number Publication date
JP3955638B2 (en) 2007-08-08
AU5489498A (en) 1998-07-03
DE69717490T2 (en) 2003-09-04
EP0946870B1 (en) 2002-11-27
ES2187834T3 (en) 2003-06-16
ATE228656T1 (en) 2002-12-15
FR2757162B1 (en) 1999-03-26
CA2272890C (en) 2004-11-30
DE69717490D1 (en) 2003-01-09
JP2001506002A (en) 2001-05-08
EP0946870A1 (en) 1999-10-06
WO1998026287A1 (en) 1998-06-18
FR2757162A1 (en) 1998-06-19

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