CA2272564A1 - Plante(s) transgenique(s) presentant une teneur naturelle elevee en eau et surproduisant au moins deux acides amines de la famille aspartate - Google Patents

Plante(s) transgenique(s) presentant une teneur naturelle elevee en eau et surproduisant au moins deux acides amines de la famille aspartate Download PDF

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Publication number
CA2272564A1
CA2272564A1 CA002272564A CA2272564A CA2272564A1 CA 2272564 A1 CA2272564 A1 CA 2272564A1 CA 002272564 A CA002272564 A CA 002272564A CA 2272564 A CA2272564 A CA 2272564A CA 2272564 A1 CA2272564 A1 CA 2272564A1
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CA
Canada
Prior art keywords
plant
sequence
gene
expression
gene construct
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002272564A
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English (en)
Inventor
Ingrid Maria Van Der Meer
Oscar Frederik Jozef Vorst
Peter Martin Bruinenberg
Johannes Pieter Marinus Sanders
Adrianus Johannes Van Tunen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cooperative Avebe UA
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2272564A1 publication Critical patent/CA2272564A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8253—Methionine or cysteine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention porte sur une construction de gène double chimère, comprenant une séquence d'acides nucléiques codant une enzyme possédant une activité d'aspartate kinase (AK), ainsi qu'une séquence d'acides nucléiques codant une enzyme possédant une activité de dihydrodipicolinate synthase (DHPS). Cette construction peut faire s'exprimer de manière différentielle les deux gènes, dans une plante ou dans des parties de celle-ci, avec pour conséquence l'obtention d'un taux accru à la fois de lysine et de thréonine, ce taux étant cinq fois supérieur au taux de type sauvage de chaque acide aminé.
CA002272564A 1996-11-18 1996-11-18 Plante(s) transgenique(s) presentant une teneur naturelle elevee en eau et surproduisant au moins deux acides amines de la famille aspartate Abandoned CA2272564A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/NL1996/000458 WO1998022600A1 (fr) 1996-11-18 1996-11-18 Plante(s) transgenique(s) presentant une teneur naturelle elevee en eau et surproduisant au moins deux acides amines de la famille aspartate

Publications (1)

Publication Number Publication Date
CA2272564A1 true CA2272564A1 (fr) 1998-05-28

Family

ID=19866010

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002272564A Abandoned CA2272564A1 (fr) 1996-11-18 1996-11-18 Plante(s) transgenique(s) presentant une teneur naturelle elevee en eau et surproduisant au moins deux acides amines de la famille aspartate

Country Status (8)

Country Link
EP (1) EP0941351A1 (fr)
AU (1) AU7509096A (fr)
CA (1) CA2272564A1 (fr)
CZ (1) CZ174699A3 (fr)
EE (1) EE9900198A (fr)
SK (1) SK65299A3 (fr)
WO (1) WO1998022600A1 (fr)
ZA (1) ZA9710382B (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1074629A1 (fr) * 1999-07-30 2001-02-07 Coöperatieve Verkoop- en Productievereniging van Aardappelmeel en Derivaten 'AVEBE' B.A. Protéines pièges
CN102076856B (zh) 2008-06-30 2014-12-31 孟山都技术公司 用于提高植物中的氨基酸含量的组合物和方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5367110A (en) * 1990-11-13 1994-11-22 Yeda Research And Development Co. Ltd. Transgenic plants overproducing threonine and lysine
AU661334B2 (en) * 1991-08-09 1995-07-20 E.I. Du Pont De Nemours And Company Synthetic storage proteins with defined structure containing programmable levels of essential amino acids for improvement of the nutritional value of plants
CA2132414C (fr) * 1992-03-19 2009-01-27 Saverio Carl Falco Fragments d'acide nucleique et methodes pour accroitre la teneur en lysine et en threonine des graines de plantes
EP0687303B1 (fr) * 1993-03-02 2002-11-20 E.I. Du Pont De Nemours And Company Augmentation du contenu en methionine dans les semances des plantes par l'expression de la 10kd zeine du mais
AU704510B2 (en) * 1993-11-30 1999-04-22 E.I. Du Pont De Nemours And Company Chimeric genes and methods for increasing the lysine content of the seeds of corn, soybean and rapeseed plants

Also Published As

Publication number Publication date
EE9900198A (et) 1999-12-15
EP0941351A1 (fr) 1999-09-15
AU7509096A (en) 1998-06-10
WO1998022600A1 (fr) 1998-05-28
CZ174699A3 (cs) 1999-09-15
SK65299A3 (en) 2000-05-16
ZA9710382B (en) 1998-06-10

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued