CA2266755A1 - Methode de sequencage de polymeres d'acides nucleiques - Google Patents

Methode de sequencage de polymeres d'acides nucleiques Download PDF

Info

Publication number
CA2266755A1
CA2266755A1 CA002266755A CA2266755A CA2266755A1 CA 2266755 A1 CA2266755 A1 CA 2266755A1 CA 002266755 A CA002266755 A CA 002266755A CA 2266755 A CA2266755 A CA 2266755A CA 2266755 A1 CA2266755 A1 CA 2266755A1
Authority
CA
Canada
Prior art keywords
nucleic acid
sequencing
sample
nucleotide
reaction mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002266755A
Other languages
English (en)
Inventor
James M. Dunn
Marina T. Larson
James Leushner
May Hui
Jean-Michel Lacroix
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
Original Assignee
Visible Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Visible Genetics Inc filed Critical Visible Genetics Inc
Publication of CA2266755A1 publication Critical patent/CA2266755A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178Special arrangements of analysers
    • G01N2035/00237Handling microquantities of analyte, e.g. microvalves, capillary networks
CA002266755A 1998-04-24 1999-03-25 Methode de sequencage de polymeres d'acides nucleiques Abandoned CA2266755A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6574898A 1998-04-24 1998-04-24
US09/065,748 1998-04-24

Publications (1)

Publication Number Publication Date
CA2266755A1 true CA2266755A1 (fr) 1999-10-24

Family

ID=22064842

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002266755A Abandoned CA2266755A1 (fr) 1998-04-24 1999-03-25 Methode de sequencage de polymeres d'acides nucleiques

Country Status (3)

Country Link
CA (1) CA2266755A1 (fr)
DE (1) DE19917871A1 (fr)
FR (1) FR2778414B1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2266755A1 (fr) * 1998-04-24 1999-10-24 James M. Dunn Methode de sequencage de polymeres d'acides nucleiques

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992001814A2 (fr) * 1990-07-24 1992-02-06 F. Hoffmann-La-Roche Ag Reduction d'amplification non-specifique au cours d'une amplification in vitro d'acide nucleique utilisant des bases d'acide nucleique modifiees
DE655506T1 (de) * 1994-10-17 1995-09-28 Harvard College DNS Polymerase mit Veränderten Nukleotiden Bindungstelle.
GB9524974D0 (en) * 1995-12-06 1996-02-07 Amersham Int Plc An improved method for dye-terminator (cycle) sequencing
EP0914468B1 (fr) * 1996-05-01 2002-08-28 Visible Genetics Inc. Procede de sequencage de polymeres d'acide nucleique
CA2266755A1 (fr) * 1998-04-24 1999-10-24 James M. Dunn Methode de sequencage de polymeres d'acides nucleiques

Also Published As

Publication number Publication date
FR2778414B1 (fr) 2003-01-24
FR2778414A1 (fr) 1999-11-12
DE19917871A1 (de) 1999-11-11

Similar Documents

Publication Publication Date Title
EP0907752B1 (fr) Procede de determination de sequences d'acides nucleiques et leurs applications diagnostiques
US6083699A (en) Method for bi-directional sequencing of nucleic acid polymers
US5834189A (en) Method for evaluation of polymorphic genetic sequences, and the use thereof in identification of HLA types
US6280954B1 (en) Arrayed primer extension technique for nucleic acid analysis
CA2233079C (fr) Procede de caracterisation de molecules d'acide nucleique
US6458584B1 (en) Customized oligonucleotide microchips that convert multiple genetic information to simple patterns, are portable and reusable
EP0914468B1 (fr) Procede de sequencage de polymeres d'acide nucleique
US5789168A (en) Method for amplification and sequencing of nucleic acid polymers
CA2374406A1 (fr) Analyse des variations de sequences polynucleotidiques par micro-echantillons
EP0951569A2 (fr) Micropuces oligonucleotidiques sur mesure utilisees comme capteurs multiples
US5910413A (en) Method and kit for amplification, sequencing and typing of classical HLA class I genes
US6225062B1 (en) Method and kit for direct isothermal sequencing of nucleic acids
US20020018999A1 (en) Methods for characterizing polymorphisms
US6413718B1 (en) Method for sequencing of nucleic acid polymers
CA2266755A1 (fr) Methode de sequencage de polymeres d'acides nucleiques
WO2021182850A1 (fr) Procédé de détection simultanée de multiples miarn et kit de détection de miarn l'utilisant
WO1998026091A2 (fr) Procede et trousse servant a determiner le type de hla de classe i
US7195877B2 (en) Cytochrome P450 genetic variations
CN111593115A (zh) 用于β-地中海贫血基因突变多重实时荧光PCR检测的引物和探针组合及试剂盒
US5643724A (en) Methods and reagents for Glycophorin A typing
Saunders et al. DNA amplification: general concepts and methods
WO2004009760A2 (fr) Variations genetiques du cytochrome p 450
EP0575845A2 (fr) Procédé de détermination de type de HLA-Dp
JP2005198525A (ja) グルタチオンs−トランスフェラーゼ遺伝子多型の検出方法および検出用キット

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued