CA2265270A1 - Liquid prothrombin time reagent - Google Patents
Liquid prothrombin time reagent Download PDFInfo
- Publication number
- CA2265270A1 CA2265270A1 CA002265270A CA2265270A CA2265270A1 CA 2265270 A1 CA2265270 A1 CA 2265270A1 CA 002265270 A CA002265270 A CA 002265270A CA 2265270 A CA2265270 A CA 2265270A CA 2265270 A1 CA2265270 A1 CA 2265270A1
- Authority
- CA
- Canada
- Prior art keywords
- reagent
- human
- tissue factor
- serum albumin
- origin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The present invention relates to a ready-to-use prothrombin time reagent which is stable long-term, based on recombinant tissue factor, and its use in clotting tests.
Claims (12)
1. A ready-to-use prothrombin time reagent which is stable long-term, based on recombinant tissue factor.
2. The reagent as claimed in claim 1, which has a life of at least 12 months in a closed container and at 2-8°C.
3. The reagent as claimed in claim 1, which contains at least one antioxidant and additionally a serum albumin.
4. The reagent as claimed in claim 3, wherein ascorbic acid is added as an antioxidant.
5. The reagent as claimed in claim 4, wherein ascorbic acid is used in a concentration of 1 µM
to 0.1 M.
to 0.1 M.
6. The reagent as claimed in claim 3, wherein the serum albumin is of human, animal or recombinant origin.
7. The reagent as claimed in claim 6, wherein the serum albumin is of human origin.
8. The reagent as claimed in claim 7, wherein the human serum albumin is added in a concentration of 0.1 g/1 to 100 g/1.
9. The reagent as claimed in claim 1, wherein the recombinant tissue factor used is of human or animal origin.
10. The reagent as claimed in claim 9, which is a human recombinant tissue factor.
11. The reagent as claimed in claim 9, wherein the tissue factor is cloned and expressed in cells of human, animal, vegetable, fungal or microbial origin.
12. The reagent as claimed in claim 11, wherein the host cell is Escherichia coli.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19811016.2 | 1998-03-13 | ||
DE19811016A DE19811016A1 (en) | 1998-03-13 | 1998-03-13 | Ready-to-use prothrombin time reagent based on recombinant tissue factor |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2265270A1 true CA2265270A1 (en) | 1999-09-13 |
CA2265270C CA2265270C (en) | 2009-11-24 |
Family
ID=7860840
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002263197A Withdrawn CA2263197A1 (en) | 1998-03-13 | 1999-03-01 | Ready-to-use prothrombin time reagent based on recombinant tissue factor |
CA002265270A Expired - Lifetime CA2265270C (en) | 1998-03-13 | 1999-03-11 | Liquid prothrombin time reagent |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002263197A Withdrawn CA2263197A1 (en) | 1998-03-13 | 1999-03-01 | Ready-to-use prothrombin time reagent based on recombinant tissue factor |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0942284B1 (en) |
JP (1) | JP4199365B2 (en) |
AT (1) | ATE385315T1 (en) |
CA (2) | CA2263197A1 (en) |
DE (2) | DE19811016A1 (en) |
ES (1) | ES2299224T3 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10877052B2 (en) | 2016-02-12 | 2020-12-29 | Instrumentation Laboratory Company | Two component “mix and use” liquid thromboplastin reagent, methods of making, and methods of use thereof |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7049087B2 (en) * | 2002-11-05 | 2006-05-23 | Lifescan, Inc. | Method for manufacturing a tissue factor-based prothrombin time reagent |
JP4377207B2 (en) * | 2003-11-28 | 2009-12-02 | シスメックス株式会社 | Blood coagulation time measurement method and blood coagulation time measurement reagent |
US7148067B2 (en) * | 2004-08-31 | 2006-12-12 | The Board Of Trustees Of The University Of Illinois | Thromboplastin reagents |
WO2006096345A2 (en) | 2005-03-04 | 2006-09-14 | The Board Of Trustees Of The University Of Illinois | Coagulation and fibrinolytic cascades modulator |
US8821861B2 (en) | 2007-10-05 | 2014-09-02 | The Board Of Trustees Of The University Of Illinois | Fibrin sealant |
WO2009061697A1 (en) | 2007-11-09 | 2009-05-14 | The Board Of Trustees Of The University Of Illinois | Anticoagulant antagonist and hemophilia procoagulant |
DE102007062323A1 (en) | 2007-12-21 | 2009-06-25 | Siemens Healthcare Diagnostics Products Gmbh | Long-term stable thromboplastin reagent |
US9028808B2 (en) | 2010-01-28 | 2015-05-12 | Immunservice Gmbh | Methods for treating nicotine addiction and reducing food consumption |
JP6116231B2 (en) * | 2012-12-25 | 2017-04-19 | 株式会社Lsiメディエンス | Tissue thromboplastin-containing blood coagulation ability measuring reagent and measuring method |
ES2617612T3 (en) * | 2013-02-28 | 2017-06-19 | Siemens Healthcare Diagnostics Products Gmbh | Procedure for the determination of protein S activity. |
JP6250306B2 (en) * | 2013-05-28 | 2017-12-20 | 株式会社Lsiメディエンス | Respirated thromboplastin-containing blood coagulation ability measuring reagent and measuring method |
EP3489692A1 (en) * | 2017-11-28 | 2019-05-29 | Siemens Healthcare Diagnostics Products GmbH | Prothrombin time reagent comprising an iron chelator |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3522148A (en) * | 1965-08-13 | 1970-07-28 | Dade Reagents Inc | Stabilized thromboplastin preparation |
US5314695A (en) * | 1990-11-13 | 1994-05-24 | Corvas International, Inc. | Tissue factor based prothrombin time reagent |
US5358853A (en) * | 1992-08-03 | 1994-10-25 | Akzo Av | Liquid thromboplastin reagent |
DE69832628T2 (en) * | 1997-04-23 | 2006-09-07 | Instrumentation Laboratory S.P.A. | RECOMBINANT RABBIT FACTOR BASED PROTHROMBIN TIME REAGENT |
-
1998
- 1998-03-13 DE DE19811016A patent/DE19811016A1/en not_active Withdrawn
-
1999
- 1999-02-12 DE DE59914634T patent/DE59914634D1/en not_active Expired - Lifetime
- 1999-02-12 EP EP99102672A patent/EP0942284B1/en not_active Expired - Lifetime
- 1999-02-12 ES ES99102672T patent/ES2299224T3/en not_active Expired - Lifetime
- 1999-02-12 AT AT99102672T patent/ATE385315T1/en not_active IP Right Cessation
- 1999-03-01 CA CA002263197A patent/CA2263197A1/en not_active Withdrawn
- 1999-03-11 CA CA002265270A patent/CA2265270C/en not_active Expired - Lifetime
- 1999-03-12 JP JP06585699A patent/JP4199365B2/en not_active Expired - Fee Related
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10877052B2 (en) | 2016-02-12 | 2020-12-29 | Instrumentation Laboratory Company | Two component “mix and use” liquid thromboplastin reagent, methods of making, and methods of use thereof |
US11460477B2 (en) | 2016-02-12 | 2022-10-04 | Instrumentation Laboratory Company | Two component “mix and use” liquid thromboplastin reagent, methods of making, and methods of use thereof |
Also Published As
Publication number | Publication date |
---|---|
EP0942284A3 (en) | 2005-03-16 |
ES2299224T3 (en) | 2008-05-16 |
EP0942284A2 (en) | 1999-09-15 |
JPH11316228A (en) | 1999-11-16 |
EP0942284B1 (en) | 2008-01-30 |
DE19811016A1 (en) | 1999-09-16 |
CA2263197A1 (en) | 1999-09-13 |
DE59914634D1 (en) | 2008-03-20 |
JP4199365B2 (en) | 2008-12-17 |
CA2265270C (en) | 2009-11-24 |
ATE385315T1 (en) | 2008-02-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |
Effective date: 20190311 |